CN221460405U - High-capacity multi-cell co-culture device - Google Patents
High-capacity multi-cell co-culture device Download PDFInfo
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- CN221460405U CN221460405U CN202323079074.2U CN202323079074U CN221460405U CN 221460405 U CN221460405 U CN 221460405U CN 202323079074 U CN202323079074 U CN 202323079074U CN 221460405 U CN221460405 U CN 221460405U
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- 238000003501 co-culture Methods 0.000 title claims abstract description 41
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 229920000515 polycarbonate Polymers 0.000 claims abstract description 8
- 239000004417 polycarbonate Substances 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 abstract description 7
- 230000003068 static effect Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229920006289 polycarbonate film Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model relates to a large-capacity multi-cell co-culture device which comprises a substrate, a left side bracket, an independent culture tube, a middle bracket, a supporting device, an indirect co-culture tube, a polycarbonate membrane, a right side bracket, a direct co-culture tube and a fixing device. Through setting up movable strutting arrangement, make the transparent blake bottle that is equipped with the culture solution clear up fast, improve the efficiency of changing the culture solution, raise upward through the use of transparent blake bottle, and take out and place the piece, can directly pour out the inside culture solution of transparent blake bottle, the convenience is strong, through setting up fixing device, utilize the sucking disc to hold the workstation, and then it is fixed with the culture apparatus, receive external force to take place to empty when preventing that cell culture is static, cause the phenomenon of damage, and the threaded connection of accessible movable frame and locating part, upwards rotate the sucking disc, leave the working face, steadily vacate, when avoiding operating the culture apparatus, the sucking disc catches the working face, remove the difficulty, cause the phenomenon of operation difficulty.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a large-capacity multi-cell co-culture device.
Background
The cell culture is to put the cells in vitro for growth and reproduction, and has the advantages of direct observation of the morphological structure and life activity of living cells, direct observation of the change of the cells, easy provision of a large number of experimental objects with similar biological properties, low cost and the like, and the in vitro culture of the cells can be single cell culture and multiple cell co-culture.
In the Chinese patent of the utility model with the application number of CN202022527301.3, a multi-cell co-culture device is disclosed, the problem that the existing multi-cell co-culture device can only perform one or two system culture, and is inconvenient to compare co-culture is solved, a co-culture cylinder in the device is fixed at the bottom end of a substrate, when culture fluid in the co-culture cylinder needs to be cleaned and replaced, the operation is inconvenient, the culture fluid is not easy to be cleaned out quickly, and the device is placed on a working table surface only by gravity in the process of placing and waiting for cell culture, and is easy to be dumped by external force, so that the culture experiment is damaged.
Disclosure of utility model
(One) solving the technical problems
In order to solve the technical problems, the utility model provides a large-capacity multi-cell co-culture device.
(II) technical scheme
Based on the above, the utility model provides the following technical scheme: the utility model provides a multiple cell co-culture device of large capacity, includes base plate, left side support, independent cultivation pipe, middle part support, strutting arrangement, indirect co-cultivation pipe, polycarbonate membrane, right side support, direct co-cultivation pipe, fixing device, the bottom middle part of base plate is fixed with the middle part support, left side support and right side support are fixed in the left and right sides bottom of base plate respectively, independent cultivation pipe is placed in the inside of left side support, the bottom of indirect co-cultivation pipe is provided with the polycarbonate membrane, direct co-cultivation pipe is placed in the inside of right side support, strutting arrangement is connected with the inside of middle part support, and the middle part has placed indirect co-cultivation pipe, fixing device's left end is fixed with right side support mutually, strutting arrangement includes transparent culture bottle, gag lever post, accessory plate, connecting pad, places the piece, transparent culture bottle is placed in the inside of middle part support, and its bottom is fixed with the gag lever post, the top of connecting pad is fixed in the inboard of transparent culture bottle, place the piece embedding transparent culture bottle top, and its outer side is connected with the connecting piece and is placed the inside and is jointly cultivated the piece and is connected with the indirect co-cultivation pipe.
Preferably, the fixing device comprises a mounting seat, a limiting part, a movable frame and a sucker, wherein the left end of the mounting seat is fixed with the right side support, the top end of the mounting seat is fixed with the limiting part, the movable frame moves outside the limiting part, and the sucker is fixed at the bottom end of the movable frame.
Preferably, the top of transparent blake bottle is provided with the accessory plate, is convenient for take transparent blake bottle, dismantlement.
Preferably, a plurality of groups of circular through holes are formed in the placement block, so that a plurality of groups of indirect co-culture pipes can be placed conveniently.
Preferably, a chute is arranged on the inner side of the top end of the transparent culture bottle.
Preferably, threads are arranged on the outer side of the upper end of the limiting piece.
Preferably, the left end of movable frame is the ring form, and this ring inner wall is provided with the screw thread, with the upper end threaded connection of locating part, is convenient for fix the sucking disc spiral, when avoiding the operation, the sucking disc sticks the working face, causes the condition of removal difficulty.
Preferably, the limiting piece is made of stainless steel, has high strength and is resistant to use.
Preferably, the connecting pad is made of elastic rubber, has a certain elastic force, and can enable the placing block to be stably placed in the transparent culture flask.
Preferably, an elastic rubber pad is arranged at the connecting groove of the base plate and the limiting rod, so that the stable connection of the base plate and the limiting rod is convenient to strengthen.
(III) beneficial effects
Compared with the prior art, the utility model provides a large-capacity multi-cell co-culture device, which has the following beneficial effects:
1. This multi-cell co-culture device of large capacity, through setting up movable strutting arrangement, make the transparent blake bottle that is equipped with the culture solution clear up fast, improve the efficiency of changing the culture solution, upwards lift up the transparent blake bottle with the power to take out and place the piece, can directly pour out the inside culture solution of transparent blake bottle, the convenience is strong.
2. This a multiple cell co-culture device of large capacity utilizes the sucking disc to hold the workstation through setting up fixing device, and then fixes culture device, receives external force to take place to empty when preventing that cell culture is static, causes the phenomenon of damage to the threaded connection of accessible movable frame and locating part upwards rotates the sucking disc, leaves the working face, steadily vacates, when avoiding operating culture device, the sucking disc holds the working face, removes the difficulty, causes the phenomenon of difficult operation.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic cross-sectional view of the present utility model;
FIG. 3 is a schematic cross-sectional view of a supporting device according to the present utility model;
FIG. 4 is a schematic perspective view of a fixing device according to the present utility model;
FIG. 5 is a schematic cross-sectional view of a fixing device according to the present utility model.
In the figure: the device comprises a base plate-1, a left side bracket-2, an independent culture tube-3, a middle bracket-4, a supporting device-5, an indirect co-culture tube-6, a polycarbonate membrane-7, a right side bracket-8, a direct co-culture tube-9, a fixing device-10, a transparent culture bottle-51, a limit rod-52, an auxiliary plate-53, a connecting pad-54, a placing block-55, a mounting seat-101, a limit piece-102, a movable frame-103 and a sucker-104.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-2, a large-capacity multi-cell co-culture device comprises a substrate 1, a left side bracket 2, an independent culture tube 3, a middle bracket 4, an indirect co-culture tube 6, a polycarbonate membrane 7, a right side bracket 8 and a direct co-culture tube 9, wherein the middle part of the bottom end of the substrate 1 is fixedly provided with the middle bracket 4, the left side bracket 2 and the right side bracket 8 are respectively fixed at the bottom ends of the left side and the right side of the substrate 1, the independent culture tube 3 is arranged in the left side bracket 2, the polycarbonate membrane 7 is arranged at the bottom end of the indirect co-culture tube 6, and the direct co-culture tube 9 is arranged in the right side bracket 8.
Referring to fig. 3, a large-capacity multi-cell co-culturing device comprises a supporting device 5, the supporting device 5 is connected with the inside of a middle bracket 4, and an indirect co-culturing tube 6 is placed at the middle of the supporting device 5, the supporting device 5 comprises a transparent culture bottle 51, a limiting rod 52, an auxiliary plate 53, a connecting pad 54 and a placing block 55, the transparent culture bottle 51 is placed in the middle bracket 4, the limiting rod 52 is fixed at the bottom end of the transparent culture bottle, the limiting rod 52 is embedded into the top end of a substrate 1, the connecting pad 54 is fixed on the inner side of the transparent culture bottle 51, the placing block 55 is embedded into the top end of the transparent culture bottle 51, the outer side of the supporting device is connected with the connecting pad 54, the inside of the placing block 55 is connected with the indirect co-culturing tube 6, the auxiliary plate 53 is arranged at the top end of the transparent culture bottle 51, the transparent culture bottle 51 is conveniently taken and disassembled, a plurality of groups of round through holes are formed in the inside of the placing block 55, the transparent culture bottle 51 is conveniently placed at the top end of the transparent culture bottle 51, the connecting pad 54 is made of elastic rubber, a certain elastic force is provided, the placing block 55 can be stably placed in the inside the transparent culture bottle 51, the substrate is conveniently connected with the substrate by the elastic rubber pad, and the limiting rod is conveniently connected with the limiting rod.
Referring to fig. 4-5, a large-capacity multi-cell co-culture device is disclosed, the left end of a fixing device 10 is fixed with a right side bracket 8, the fixing device 10 comprises a mounting seat 101, a limiting piece 102, a movable frame 103 and a sucker 104, the left end of the mounting seat 101 is fixed with the right side bracket 8, the top end of the mounting seat is fixed with the limiting piece 102, the movable frame 103 moves outside the limiting piece 102, the sucker 104 is fixed at the bottom end of the mounting seat, threads are arranged outside the upper end of the limiting piece 102, the left end of the movable frame 103 is in a ring shape, threads are arranged on the inner wall of the ring, the upper end of the ring is in threaded connection with the upper end of the limiting piece 102, the sucker 104 is conveniently fixed in a spiral manner, and when the operation is avoided, the sucker 104 attracts a working surface, and the limiting piece 102 is made of stainless steel, has high strength and is resistant to use.
In summary, when a plurality of cells need to be cultured independently in use, the plurality of cells are respectively injected into different independent culture tubes 3, added with culture solution for independent culture, and put into a left bracket 2 for standing differentiation; when multiple cells are required to be directly co-cultured, the multiple cells and the culture solution are co-injected into the direct co-culture tube 9 and put into the right bracket 8 for standing and differentiation, when the multiple cells are required to be separately cultured and the same culture solution is used, the multiple cells are respectively injected into different indirect co-culture tubes 6, the culture solution is injected into the transparent culture bottle 51, the multiple indirect co-culture tubes 6 are all placed in the placement block 55, the multiple indirect co-culture tubes 6 are in the same culture solution, the polycarbonate film 7 can prevent the diffusion of the cells, only the culture solution is allowed to pass, the multiple cells are independently cultured in the same culture solution, and the high-capacity multi-cell co-culture is realized;
When the culture solution in the transparent culture bottle 51 needs to be replaced, the transparent culture bottle 51 is lifted upwards by force, the limiting rod 52 is separated from the top end of the substrate 1, the placing block 55 is taken out, and the culture solution in the transparent culture bottle 51 can be directly poured out, so that the convenience is high;
moreover, when the cells need to be cultured for a long time and kept still, the sucker 104 is utilized to suck the workbench, so that the culture device is fixed, the phenomenon of damage caused by the toppling of the cells due to external force when the cell culture is still is prevented, and the sucker 104 is upwards rotated and separated from the working surface through the threaded connection of the movable frame 103 and the limiting piece 102, so that the working surface is stably emptied, and the phenomenon of difficult operation caused by difficult movement due to the sucker sucking the working surface when the culture device is prevented from being operated.
The control mode of the utility model is controlled by manually starting and closing the switch, the wiring diagram of the power element and the supply of the power supply are common knowledge in the field, and the utility model is mainly used for protecting the mechanical device, so the utility model does not explain the control mode and the wiring arrangement in detail.
The control mode of the utility model is automatically controlled by the controller, the control circuit of the controller can be realized by simple programming of a person skilled in the art, the supply of power also belongs to common knowledge in the art, and the utility model is mainly used for protecting a mechanical device, so the utility model does not explain the control mode and circuit connection in detail.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a high-capacity multiple cell co-culture device, includes base plate (1), left side support (2), independent cultivation pipe (3), middle part support (4), indirect co-cultivation pipe (6), polycarbonate membrane (7), right side support (8), direct co-cultivation pipe (9), the bottom middle part of base plate (1) is fixed with middle part support (4);
The method is characterized in that: the device is characterized by further comprising a supporting device (5) and a fixing device (10), wherein the supporting device (5) is connected with the inside of the middle support (4), an indirect co-cultivation tube (6) is placed in the middle of the supporting device, the left end of the fixing device (10) is fixed with the right support (8), the supporting device (5) comprises a transparent culture flask (51), a limiting rod (52), an auxiliary plate (53), a connecting pad (54) and a placing block (55), the transparent culture flask (51) is placed in the middle support (4), the limiting rod (52) is fixed at the bottom end of the transparent culture flask (51) and is embedded into the top end of the substrate (1), the connecting pad (54) is fixed on the inner side of the transparent culture flask (51), the placing block (55) is embedded into the top end of the transparent culture flask (51), the outer side of the placing block is connected with the connecting pad (54), and the inner side of the placing block (55) is connected with the indirect co-cultivation tube (6).
2. The high-capacity multicellular co-culture apparatus of claim 1 wherein: the fixing device (10) comprises a mounting seat (101), a limiting part (102), a movable frame (103) and a sucker (104), wherein the left end of the mounting seat (101) is fixed with a right side bracket (8), the top end of the mounting seat is fixed with the limiting part (102), the movable frame (103) moves outside the limiting part (102), and the sucker (104) is fixed at the bottom end of the movable frame.
3. The high-capacity multicellular co-culture apparatus of claim 1 wherein: the left side support (2) and the right side support (8) are respectively fixed at the left side bottom and the right side bottom of the substrate (1), the independent culture tube (3) is arranged in the left side support (2), the bottom of the indirect co-culture tube (6) is provided with a polycarbonate membrane (7), and the direct co-culture tube (9) is arranged in the right side support (8).
4. The high-capacity multicellular co-culture apparatus of claim 1 wherein: an auxiliary plate (53) is arranged at the top end of the transparent culture bottle (51).
5. The high-capacity multicellular co-culture apparatus of claim 1 wherein: a plurality of groups of circular through holes are formed in the placement block (55).
6. The high-capacity multicellular co-culture apparatus of claim 1 wherein: a chute is arranged on the inner side of the top end of the transparent culture bottle (51).
7. The high-capacity multicellular co-culture apparatus of claim 2 wherein: threads are arranged on the outer side of the upper end of the limiting piece (102).
8. The high-capacity multicellular co-culture apparatus of claim 2 wherein: the left end of the movable frame (103) is in a circular ring shape, and threads are arranged on the inner wall of the circular ring and are connected with the upper end of the limiting piece (102) in a threaded mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323079074.2U CN221460405U (en) | 2023-11-14 | 2023-11-14 | High-capacity multi-cell co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323079074.2U CN221460405U (en) | 2023-11-14 | 2023-11-14 | High-capacity multi-cell co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221460405U true CN221460405U (en) | 2024-08-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202323079074.2U Active CN221460405U (en) | 2023-11-14 | 2023-11-14 | High-capacity multi-cell co-culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221460405U (en) |
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2023
- 2023-11-14 CN CN202323079074.2U patent/CN221460405U/en active Active
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