CN212560304U - Novel suspension cell culture bag is fixed and is shifted device - Google Patents
Novel suspension cell culture bag is fixed and is shifted device Download PDFInfo
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- CN212560304U CN212560304U CN202020438131.9U CN202020438131U CN212560304U CN 212560304 U CN212560304 U CN 212560304U CN 202020438131 U CN202020438131 U CN 202020438131U CN 212560304 U CN212560304 U CN 212560304U
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Abstract
The utility model relates to a novel suspension cell culture bag fixing and transferring device, which comprises a placing rack and a culture tray, wherein the placing rack comprises a bottom plate, a vertical fixing plate is vertically installed on the bottom plate, culture supporting plates are installed on the vertical fixing plate at intervals, limiting slots are symmetrically formed on the bottom plate and the culture supporting plates, the culture tray is inserted into the limiting slots, a plurality of exhaust holes are formed in the limiting slots, a top plate is installed at the top end of the vertical fixing plate, and two groups of injector through holes are symmetrically formed in two sides of the top plate; the cultivation tray is provided with tray enclosing baffles all around, small-aperture vent holes and large-aperture vent holes are formed in the cultivation tray at intervals, and a liquid guide tube placing opening and a sampling tube placing opening are formed in the enclosing baffle on one side of the cultivation tray respectively. The utility model is used for carry out batch cell culture, also can carry out fluid infusion and cell suspension and collect the experiment operation, satisfy the multi-functional usage of experiment, improved the utilization ratio of experimental apparatus greatly.
Description
Technical Field
The utility model belongs to the technical field of experiment auxiliary assembly, a device and supplementary fixing device technique are used in the experiment is collected and is collected to big batch cell is related to, especially a novel suspension cell culture bag is fixed and transfer device.
Background
The 21 st century is the century that human beings pursue health, and due to the rapid development of economy and the gradual improvement of quality of life, people gradually realize that health is the most valuable wealth of life and are more and more concerned about their health, however, due to the needs of economic development and industrial production, the environment changes, food safety and environmental health problems are endless, the quality of life is continuously reduced, and sub-health conditions gradually appear, which have seriously affected the health of people, consume a large amount of social medical resources and medical expenses, and not only few people are ill and poor due to diseases, and the construction of 'big health engineering' is inevitable. The new medicine is mainly advocated to prevent, so that the rapid maturity of the big health industry in China is promoted, the stem cells and the immune cells are promoted to gradually enter the field of view of the public, and more research institutions of researchers participate in the industry of the stem cells and the immune cells.
The immune cell therapy of tumor mainly comprises non-specific old technology represented by CIK, DC-CIK, NK and the like and specific new technology represented by CAR-T, TCR-T and needing in vitro gene modification, wherein the immune cell therapy mainly adopts a treatment method that the biological technology is adopted, human peripheral blood is adopted, stimulation is carried out in vitro, and a large amount of amplified and cultured blood is returned to the body so as to improve the immunity of the body and enhance the immune function, thereby achieving the purpose of treatment. In the current scientific research experiment and medical preparation room, cell culture bottles or cell culture bags are mostly adopted to culture and collect cells, the culture bottles and the culture bags have some inevitable defects in the using process, and 1, the volume of the culture bottles is small, the air permeability is poor, the method is suitable for small-batch experiments in laboratories and is not suitable for actual production; 2. in the using process of the culture bottle, the openings are needed one by one, the operation is inconvenient, and the experiment time is increased; 3. the currently used culture bags are mostly flexible plastic culture bags made of EVA materials, and can only be placed independently, so that a large amount of culture space of the incubator is occupied; 4. the culture bag is soft in texture, and has no supporting base in the use process, so that the operation and the transportation are inconvenient; 5. the cell culture bag has larger capacity, excessive suspension culture solution, difficult observation of cell state, easy laceration in the operation and observation processes and increased probability of cell pollution; 6. when cell culture bag cultivates the cell and carries out the fluid infusion, need carry out the fluid infusion to a plurality of culture bags one by one, and the syringe of fluid infusion pipe and addition culture solution does not have fixed device, and the operation is inconvenient, has the risk of pollution, and increases the experimental time.
The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:
1. a multi-applicability cell culture bottle storage rack (CN204589202U) comprises a rectangular bottom plate, a rectangular back plate and a triangular side plate, wherein four sides of the back plate are respectively an upper side of the back plate, a bottom side of the back plate and two side sides of the back plate, four sides of the bottom plate are respectively an upper side of the bottom plate, a bottom side of the bottom plate and two side sides of the bottom plate, the bottom side of the bottom plate is vertically and fixedly connected with the bottom side of the back plate, the side plates are fixedly connected between the side sides of the bottom plate and the side sides of the back plate, and the bottom plate, the back plate and the side plates are encircled to form an upward open; at least two triangular placing racks are uniformly arranged on the bottom surface of the back plate; the method is characterized in that: the sliding type backboard is characterized by further comprising a placing pad, wherein corresponding hook-shaped sliding grooves respectively extend upwards from two ends of the upper surface of the backboard, the sliding grooves are located on the inner sides of the side plates on two sides, a plurality of placing grooves which are distributed in parallel and are semicircular in cross section are longitudinally embedded in the upper surface of the placing pad, sliding parts corresponding to the sliding grooves respectively extend from the bottom ends of two sides of the placing pad, and the placing pad is connected to the backboard in a sliding mode in a telescopic mode through the sliding parts and the sliding grooves in a sliding fit mode.
2. A cell culture bag placing frame (CN209481696U) comprises a main body frame, wherein a plurality of trays are detachably mounted on the main body frame from top to bottom, and the trays are used for placing cell culture bags; the top end of the main body frame is provided with a cross beam, the outer side of the main body frame is provided with a culture solution perfusion device, and each cell culture bag is connected with the culture solution perfusion device through a liquid injection pipe; a plurality of the main body frames can be stacked in the same incubator.
3. The utility model provides a cell culture bottle rack (CN209144128U), includes that support, first dish and the second of placing are placed the dish, the support includes the base of bottom, the base) centre is equipped with rotatable bracing piece, be equipped with first tray on the bracing piece, be equipped with the second tray that parallels with it above the first tray, first dish of placing is fixed above first tray, the second is placed the dish and is fixed above the second tray, the upper end of the support bar is equipped with the spliced pole perpendicular with the bracing piece, and portable ring passes the cavity chamber of spliced pole.
Through the contrast of technical characteristics, above-mentioned patent literature of disclosing with the utility model discloses a technical structure is inequality, can not influence the creativity and the novelty of the utility model application.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to overcome prior art's weak point, provide a novel suspension cell culture bag is fixed and transfer device to it is little to cultivate the scale in solving current culture apparatus, and incubator space utilization is low, transport inconvenient, the infusion is cultivateed the unable support of bag and fixed scheduling problem during operation.
The utility model provides a its technical problem take following technical scheme to realize:
a novel suspension cell culture bag fixing and transferring device comprises a placing rack and a culture tray, wherein the placing rack comprises a bottom plate, a vertical fixing plate is vertically installed in the middle of the upper surface of the bottom plate, culture supporting plates are evenly and alternately installed on the vertical fixing plate, limiting slots are symmetrically formed in the bottom plate and the culture supporting plates in a whole body mode, the culture tray is inserted into the limiting slots, a plurality of exhaust holes are formed in the limiting slots, a top plate is also vertically installed at the top end of the vertical fixing plate, and two groups of injector through holes for inserting and placing injectors are symmetrically formed in the two sides of the top plate; cultivate the tray and all set up the tray all around and enclose the fender, it has small aperture air vent and large aperture bleeder vent still to have the interval system on the tray to cultivate, it still makes respectively to be used for the card to put the liquid pipe of cultivateing bag catheter, cultivateing bag catheter sampling tube and places mouth and sampling tube and place the mouth to enclose on enclosing of tray one side of cultivateing.
Moreover, the diameter of the small-aperture air vent is 1 cm-3 cm; the diameter of the large-aperture air vent is 2 cm-3 cm.
Moreover, an arc tray handle is arranged on the side wall of the enclosure between the liquid guide tube placing opening and the sampling tube placing opening.
Moreover, the placing rack and the culture tray are both made of stainless steel.
And the distance between the upper and lower adjacent culture supporting plates is less than or equal to 20 cm.
And moreover, supporting mechanisms are arranged at four corners of the lower surface of the bottom plate of the placing rack.
Moreover, the supporting mechanism comprises a supporting bottom column and a magnetic sucker, and the magnetic sucker is installed at the end part of the supporting bottom column.
And a limiting ring is also arranged on the culture supporting plate on one side of the limiting slot.
And a limit buckle is fixedly arranged in the middle of the enclosure on the other side of the culture tray.
The utility model has the advantages that:
1. the utility model is mainly used for mass cell culture and collection, can culture suspension cells in batch at one time, avoids cell residue and loss caused by repeated cell collection, ensures the collection amount of cells, and saves the experimental operation time;
2. the utility model can be used for batch cell culture, and can also be used for liquid supplement and cell suspension collection experiment operation, thereby meeting the multifunctional application of an experimental device, realizing one multi-purpose and reducing the number of experiment consumables;
3. the utility model is provided with a plurality of culture supporting plates at intervals, can be used for placing a plurality of groups of cell culture bags at the same time and is arranged on the same rack main body, thus greatly saving the culture space;
4. the utility model discloses two sets of 50ml syringe through-holes are designed respectively to the left and right sides of roof, can carry out multiunit fluid infusion operation simultaneously, design the syringe fixed orifices, need not carry out handheld operation, and the experiment operator can independently accomplish the fluid infusion operation, has avoided personnel's occupation, increases experimental efficiency;
5. the periphery of the culture tray of the utility model is designed with a baffle to avoid the falling off of the culture bag when the culture bag is taken and put, and the bottom of the culture tray is designed with big and small air holes at intervals, which can not only meet the requirement of cell culture, but also can place the culture tray under a microscope for observation to avoid scratching the culture bag and causing the pollution of cell culture solution;
6. the utility model discloses a last design of the support bottom pillar of the bottom plate lower surface of rack has magnetic chuck, what can stabilize adsorbs on the wall of incubator or superclean bench, has improved the stability of rack.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic view of the placing rack of the present invention;
FIG. 3 is a schematic view of the structure of the culture tray of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.
A novel suspension cell culture bag fixing and transferring device, which comprises a placing frame and a culture tray 6, the placing rack comprises a bottom plate 11, a vertical fixing plate 1, a culture supporting plate 8 and a top plate 2, wherein the vertical fixing plate is vertically and fixedly arranged in the middle of the upper surface of the bottom plate, culture supporting plates are uniformly arranged on the vertical fixing plate at intervals, limiting slots 17 are uniformly formed on the culture supporting plates on the two sides of the vertical fixing plate, the limiting and limiting slots are respectively provided with a plurality of vent holes 16, the size of the vent holes is 1 cm, the ventilation of carbon dioxide gas and the balance of temperature and humidity in the cell culture process are facilitated, a limiting ring 4 is further arranged on the culture supporting plate on one side of the limiting slot, and the limiting ring is matched with a limiting buckle on the culture tray and used for fixing the culture tray and avoiding the culture tray from sliding off when the culture tray is taken and placed; the bottom plate and the culture supporting plate have the same structure; the top end of the vertical fixing plate is also vertically provided with a top plate, the top plate is mainly used for temporarily placing a culture tray, the top plate comprises a top plate body, and two groups of injector through holes 3 are symmetrically formed in two sides of the top plate body and used for inserting and placing injectors during liquid supplementing experiment operation;
the culture tray is made of stainless steel or PVC materials, the tray enclosure 7 with the height of 3cm is arranged around the culture tray, the culture bag is prevented from slipping off the tray when being taken and placed and moved, a limit buckle 5 is fixedly arranged in the middle of the enclosure on one side of the culture tray and is matched with a limit ring on the culture tray, small-aperture vent holes 10 and large-aperture vent holes 9 are formed in the culture tray at intervals, the small-aperture vent holes are 1-3 cm in size and beneficial to air permeability in a cell culture process, the large-aperture vent holes are 2-3 cm in size and used for observing cells under a microscope, a liquid guide tube placing opening 15 and a sampling tube placing opening 13 are formed in the enclosure on the other side of the culture tray and are used for clamping and placing a liquid guide tube and a sampling tube on one side of the culture bag respectively, and liquid supplementing and sampling are convenient.
In the embodiment of the utility model, in order to facilitate the taking and placing of the culture support plate, an arc-shaped tray handle 14 is arranged on the side wall of the enclosure between the liquid guide tube placing opening and the sampling tube placing opening.
The utility model discloses in the implementation, the main part material of rack is the stainless steel, can clean and disinfect with 75% alcohol, and high temperature resistant, can arrange the carbon dioxide incubator in and use.
The utility model discloses in the concrete implementation, the length of cultivation layer board is 40 cm-50 cm, and the width is 25 cm-50 cm, and the distance between the adjacent cultivation layer board about should not be too big, and be not more than 20 cm at best, both can place the cultivation tray, not too much occupation space again, the getting of the single cultivation tray of being convenient for is got and is put and the observation of cell in every cultivation bag.
In the specific implementation of the utility model, in order to better fix the placing rack, the placing rack can be placed stably when being placed in an incubator or an ultra-clean workbench; supporting mechanisms are installed at four corners of the lower surface of the placing rack bottom plate and comprise supporting bottom columns and magnetic suckers 12, and the magnetic suckers are installed at the end portions of the supporting bottom columns.
The utility model discloses specific use:
when the device is used, the device can be placed in an incubator for large-capacity suspension cell culture, can also be placed beside a microscope for temporarily placing a cell culture bag, and can be placed in an ultra-clean workbench or a biological safety cabinet for fluid infusion operation or suspension cell collection operation;
when cell culture is carried out, the culture bag is placed in a culture tray, the culture tray is fixed on a culture supporting plate through a limiting buckle, then the device is adsorbed in a culture box through a magnetic sucker, and one or more layers of culture supporting plates can be installed according to experiment requirements;
when the liquid supplementing operation is carried out, the injector is placed in the through hole of the injector, the culture tray filled with the culture bag is placed on the culture supporting plate at the lower part of the top plate, and the liquid guide tube of the culture bag is connected with the opening of the injector, so that the liquid supplementing operation can be carried out;
when carrying out cell collection experiment operation, will be equipped with the culture tray of culture bag and place culture tray in roof lower part on the board, place the cell culture bag catheter via the catheter and place mouthful end and place suspension collector mouth end downwards, prepare to hold the cell suspension device, carry out the cell collection.
The device can be used for culturing cells, supplementing liquid for the cells and collecting cell suspension, and is more convenient for experimental operation; because most scientific research laboratories and medical treatment laboratories still adopt 50mL syringes to add liquid or fluid infusion in to the culture bag now, generally hand-held operation, and can only singly give a culture bag at every turn and be operated by an experimental operator, and in the device of the utility model, 2 groups of syringe through-holes are made respectively on both sides of the roof, when carrying out the liquid adding and fluid infusion operation, the operator only needs to control the liquid guide pipe mouth to be closely connected with the syringe mouth, need not to support, solve the problem that operating personnel occupy in the existing experimental device, and can carry out the fluid infusion operation simultaneously by a plurality of groups, save the operating space, improve the experimental efficiency, effectively avoid the mutual interference and pollution problem; meanwhile, due to the porous design of the culture plate and the culture tray, the air permeability in the cell culture process is guaranteed, the problem that the cells are not easy to observe under a cell microscope is solved, the culture tray is protected, the scratching damage to the culture bag is avoided, and the pollution problem of the cells in the whole process is effectively avoided.
The utility model is mainly used for mass cell culture and collection, can culture suspension cells in batch at one time, avoids cell residue and loss caused by repeated cell collection, ensures the collection amount of cells, and saves the experimental operation time;
the utility model can be used for batch cell culture, and can also be used for liquid supplement and cell suspension collection experiment operation, thereby meeting the multifunctional application of an experimental device, realizing one multi-purpose and reducing the number of experiment consumables;
the utility model is provided with a plurality of culture supporting plates at intervals, can be used for placing a plurality of groups of cell culture bags at the same time and is arranged on the same rack main body, thus greatly saving the culture space;
the utility model discloses two sets of 50ml syringe through-holes are designed respectively to the left and right sides of roof, can carry out multiunit fluid infusion operation simultaneously, design the syringe fixed orifices, need not carry out handheld operation, and the experiment operator can independently accomplish the fluid infusion operation, has avoided personnel's occupation, increases experimental efficiency;
the periphery of the culture tray of the utility model is designed with a baffle to avoid the falling off of the culture bag when the culture bag is taken and put, and the bottom of the culture tray is designed with big and small air holes at intervals, which can not only meet the requirement of cell culture, but also can place the culture tray under a microscope for observation to avoid scratching the culture bag and causing the pollution of cell culture solution;
the utility model discloses a last design of the support bottom pillar of the bottom plate lower surface of rack has magnetic chuck, what can stabilize adsorbs on the wall of incubator or superclean bench, has improved the stability of rack.
Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.
Claims (9)
1. The utility model provides a novel suspension cell culture bag is fixed and shifts device which characterized in that: the automatic injector placing device comprises a placing rack and a culture tray, wherein the placing rack comprises a bottom plate, a vertical fixing plate is vertically arranged in the middle of the upper surface of the bottom plate, culture supporting plates are uniformly arranged on the vertical fixing plate at intervals, limiting slots are symmetrically formed in the bottom plate and the culture supporting plates, the culture tray is inserted into the limiting slots, a plurality of exhaust holes are formed in the limiting slots, a top plate is also vertically arranged at the top end of the vertical fixing plate, and two groups of injector through holes for inserting and placing injectors are symmetrically formed in two sides of the top plate; cultivate the tray and all set up the tray all around and enclose the fender, it has small aperture air vent and large aperture bleeder vent still to have the interval system on the tray to cultivate, it still makes respectively to be used for the card to put the liquid pipe of cultivateing bag catheter, cultivateing bag catheter sampling tube and places mouth and sampling tube and place the mouth to enclose on enclosing of tray one side of cultivateing.
2. The novel suspension cell culture bag holding and transferring device of claim 1, wherein: the diameter of the small-aperture vent hole is 1 cm-3 cm; the diameter of the large-aperture air vent is 2 cm-3 cm.
3. The novel suspension cell culture bag holding and transferring device of claim 1, wherein: an arc tray handle is arranged on the side wall of the enclosure between the liquid guide tube placing opening and the sampling tube placing opening.
4. The novel suspension cell culture bag holding and transferring device of claim 1, wherein: the placing rack and the culture tray are made of stainless steel materials.
5. The novel suspension cell culture bag holding and transferring device of claim 1, wherein: the distance between the upper and lower adjacent culture supporting plates is less than or equal to 20 cm.
6. The novel suspension cell culture bag holding and transferring device of claim 1, wherein: and supporting mechanisms are arranged at four corners of the lower surface of the bottom plate of the placing rack.
7. The novel suspension cell culture bag holding and transferring device of claim 6, wherein: the supporting mechanism comprises a supporting bottom column and a magnetic sucker, and the magnetic sucker is installed at the end part of the supporting bottom column.
8. The novel suspension cell culture bag holding and transferring device of claim 1, wherein: and a limiting ring is also arranged on the culture supporting plate on one side of the limiting slot.
9. The novel suspension cell culture bag holding and transferring device of claim 1, wherein: and a limit buckle is fixedly arranged in the middle of the enclosure at the other side of the culture tray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020438131.9U CN212560304U (en) | 2020-03-31 | 2020-03-31 | Novel suspension cell culture bag is fixed and is shifted device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020438131.9U CN212560304U (en) | 2020-03-31 | 2020-03-31 | Novel suspension cell culture bag is fixed and is shifted device |
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| Publication Number | Publication Date |
|---|---|
| CN212560304U true CN212560304U (en) | 2021-02-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020438131.9U Active CN212560304U (en) | 2020-03-31 | 2020-03-31 | Novel suspension cell culture bag is fixed and is shifted device |
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| CN (1) | CN212560304U (en) |
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- 2020-03-31 CN CN202020438131.9U patent/CN212560304U/en active Active
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