CN221296904U

CN221296904U - Liquid extraction and collection device and gene sequencer

Info

Publication number: CN221296904U
Application number: CN202323117185.8U
Authority: CN
Inventors: 王远; 王国伟; 张彤; 孟佳
Original assignee: Zhengzhou Sikun Biological Engineering Co ltd
Current assignee: Zhengzhou Sikun Biological Engineering Co ltd
Filing date: 2023-11-17
Publication date: 2024-07-09
Anticipated expiration: 2033-11-17

Abstract

The application provides a liquid extracting and collecting device and a gene sequencer. Wherein, the liquid storage box can hold buffer box and waste liquid box. The waste liquid pipe connecting piece is used for fixing the waste liquid pipe, and the liquid sucking needle is used for sucking the buffer liquid. The lifting device is used for driving the liquid suction needle and the waste liquid pipe connecting piece to lift and cooperate with the buffer liquid box and the waste liquid box in the liquid storage box to finish the extraction of the buffer liquid and the collection of the waste liquid. In the scheme, the liquid storage box integrates the waste liquid chamber and the buffer liquid chamber, and the liquid suction needle and the waste liquid pipe communicating part are integrated at the two sides of the lifting table, so that the integrated design of the buffer liquid storage device, the buffer liquid extraction device and the waste liquid collection device is realized, and the internal space of the gene sequencer is saved; the buffer solution box and the waste liquid box can be replaced simultaneously after the lifting platform is lifted, so that the operation flow of replacing the liquid storage box is simplified, and the operation convenience of the gene sequencer is improved.

Description

Liquid extraction and collection device and gene sequencer

Technical Field

The application relates to the technical field of gene sequencing, in particular to a liquid extraction and collection device and a gene sequencer.

Background

One development direction of the current gene sequencer aims at applying the gene sequencing technology to smaller and more convenient equipment, so that the gene sequencer is convenient to use in laboratories, clinics, even families and other scenes, and the popularity and convenience of the gene sequencer are improved.

The existing gene sequencer buffer storage device and the waste liquid storage device are designed independently, the integration degree is low, the occupied space is large, and the operation flow for replacing the liquid storage box is complex.

Disclosure of utility model

The application provides a liquid extraction and collection device, which is used for saving the internal space of a gene sequencer and simplifying the operation flow of replacing a liquid storage box.

In a first aspect, the present application resides in a liquid extraction and collection device for use in a gene sequencer, the liquid extraction and collection device comprising:

the liquid storage box comprises a buffer solution chamber capable of containing the buffer solution box and a waste solution chamber capable of containing the waste solution box, and the buffer solution chamber and the waste solution chamber are arranged side by side along a first direction;

The lifting device comprises a fixed frame and a lifting table; the fixing frame and the liquid storage box are arranged in a stacked mode along the second direction and fixedly connected; the lifting table is in sliding connection with the fixing frame, and can slide along the second direction and stay to a first height and a second height; the second direction is perpendicular to the first direction;

The number of the liquid sucking needles is at least one, each liquid sucking needle is fixed on one side of the lifting table facing the buffer solution chamber, and one end of each liquid sucking needle is communicated with one buffer solution pipe of the gene sequencer; when the lifting platform is at the first height, each liquid sucking needle is withdrawn out of the buffer box; when the lifting platform is at the second height, each liquid sucking needle enters the buffer solution box and reaches a set depth in the buffer solution box;

The waste liquid pipe connecting piece, the waste liquid pipe connecting piece is fixed in the elevating platform is towards waste liquid room one side, the waste liquid pipe connecting piece is used for the intercommunication gene sequencer waste liquid pipe and will the waste liquid that the gene sequencer produced is directed to the waste liquid room.

In the scheme, the buffer chamber for accommodating the buffer box and the waste liquid chamber for accommodating the waste liquid box are integrated in one liquid storage box, and the liquid suction needle and the waste liquid pipe communicating part are integrated at two sides of the lifting table, so that the integrated design of the buffer storage device, the buffer extraction device and the waste liquid collecting device is realized, and the internal space of the gene sequencer is saved; meanwhile, after the lifting platform lifts, the buffer solution box and the waste liquid box can be replaced at the same time, so that the operation flow of replacing the liquid box is simplified, and the operation convenience of the gene sequencer is improved.

In one possible embodiment, the cartridge further comprises a middle partition; the middle partition separates the waste liquid chamber from the buffer liquid chamber.

In one possible implementation mode, limiting plungers are arranged in the waste liquid chamber, the buffer liquid chamber and the middle partition plate;

The limit plunger is used for being abutted with the buffer box and the waste liquid box and limiting the buffer box to be located at a first set position in the buffer chamber and limiting the waste liquid box to be located at a second set position in the waste liquid chamber.

In one possible embodiment, a guide hole is formed in the buffer chamber on the side facing the lifting table;

The number of the guide holes is the same as that of the liquid sucking needles, the positions of the guide holes are in one-to-one correspondence with those of the liquid sucking needles, and each liquid sucking needle can penetrate through the corresponding guide hole to stretch into or withdraw from the buffer solution chamber.

In a possible embodiment, the buffer chamber is provided with identification means for identifying the identification information of the buffer cartridge.

In one possible embodiment, the waste pipe connector comprises a connecting disc fixedly connected with the lifting platform; at least one waste liquid pipe hole is formed in the connecting disc, and each waste liquid pipe hole is communicated with one waste liquid pipe;

A through hole is formed in one side, facing the lifting table, of the waste liquid chamber;

The connecting disc can slide along the lifting table to lift along the second direction; when the lifting platform is lowered to the second height, the connecting disc can penetrate through the through hole to be attached to the box opening of the waste liquid box.

In a possible embodiment, the waste pipe connection is further provided with a liquid level detection device;

The liquid level detection device is opposite to the box opening of the waste liquid box and is used for detecting the liquid level in the waste liquid box.

In one possible embodiment, the liquid extraction and collection device further comprises a drive device;

The driving device can drive the lifting platform to slide between the first height and the second height along the second direction.

In one possible embodiment, a safety device is arranged on the fixing frame;

The safety device is used for limiting the sliding track range of the lifting platform between the first height and the second height.

In a second aspect, the present application provides a gene sequencer comprising at least one buffer tube, at least one waste tube and any one of the liquid extraction and collection devices described above; wherein:

at least one buffer liquid pipe is communicated with the liquid suction needles in a one-to-one correspondence manner, and at least one waste liquid pipe is communicated with the waste liquid pipe holes in a one-to-one correspondence manner.

Drawings

FIG. 1 is a schematic view of a liquid extraction and collection device according to the present application;

FIG. 2 is a schematic view of another embodiment of a liquid extraction and collection device;

FIG. 3 is a schematic diagram of a liquid storage box according to an embodiment of the application;

FIG. 4 is a schematic diagram of a waste pipe connector according to an embodiment of the present application.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present application will be further described in detail with reference to the accompanying drawings.

It is noted that unless otherwise defined, technical or scientific terms used in one or more embodiments of the present disclosure should be taken in a general sense as understood by one of ordinary skill in the art to which the present disclosure pertains. The use of the terms "first," "second," and the like in one or more embodiments of the present description does not denote any order, quantity, or importance, but rather the terms "first," "second," and the like are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that elements or items preceding the word are included in the element or item listed after the word and equivalents thereof, but does not exclude other elements or items. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", etc. are used merely to indicate relative positional relationships, which may also be changed when the absolute position of the object to be described is changed.

In order to facilitate understanding of the liquid extracting and collecting device provided by the embodiment of the application, an application scenario thereof is first described. The liquid extraction and collection device provided by the embodiment of the application is applied to a gene sequencer, and can be particularly applied to storage and extraction of buffer liquid and collection and storage of waste liquid.

The gene sequencer needs to use buffer solution in the working process, and waste liquid can be generated. The existing gene sequencer is generally designed with a waste liquid collecting device and a buffer liquid storing and extracting device independently, which occupies a larger space in the gene sequencer and is not beneficial to the miniaturization design of the gene sequencer; and the existing gene sequencer needs two more devices to be operated respectively when the buffer solution box and the waste liquid box are replaced, and the operation flow is complex.

Based on the above, the application provides a liquid extracting and collecting device, which achieves the purposes of saving the internal space of a gene sequencer and simplifying the operation flow of replacing a liquid storage box by integrating a buffer storage device, a buffer extracting device and a waste liquid collecting device.

Referring to fig. 1 and fig. 2 together, fig. 1 is a schematic view of an overall liquid extracting and collecting device according to the present application, and fig. 2 is a schematic view of another perspective view of the liquid extracting and collecting device according to an embodiment of the present application. For convenience in describing the liquid extracting and collecting device provided by the application, a first direction, a second direction and a third direction which are perpendicular to each other are defined in a three-dimensional space. And the first direction and the third direction as shown in fig. 1 or fig. 2 are both directions parallel to the horizontal plane.

The application provides a liquid extracting and collecting device which comprises a liquid storage box 1, a lifting device 2, a liquid sucking needle 3 and a waste liquid pipe connecting piece 4. Wherein the liquid storage box 1 can accommodate a buffer liquid box and a waste liquid box. The waste liquid pipe connecting piece 4 is used for fixing the waste liquid pipe, and the liquid sucking needle 3 is used for sucking the buffer solution. The lifting device 2 is used for driving the liquid suction needle 3 and the waste liquid pipe connecting piece 4 to lift and complete the work of extracting the buffer liquid and collecting the waste liquid in cooperation with the buffer liquid box and the waste liquid box in the liquid storage box 1.

Specifically, the cartridge 1 includes a buffer chamber 11 that can accommodate a buffer cartridge and a waste chamber 12 that can accommodate a waste cartridge, and the buffer chamber 11 and the waste chamber 12 are arranged side by side in the first direction. The buffer chamber 11 and the waste liquid chamber 12 may be surrounded by a plate. And in the one end of the third direction perpendicular to the first direction, the buffer solution chamber 11 and the waste solution chamber 12 surrounded by the plate are provided with openings, and the buffer solution box and the waste solution box can be placed into the liquid storage box 1 or taken out from the liquid storage box 1 along the third direction from the openings.

The lifting device 2 comprises a fixed frame 21 and a lifting table 22. The fixing frame 21 and the liquid storage box 1 are stacked along a second direction perpendicular to the first direction, and the fixing frame 21 is fixedly connected with the liquid storage box 1.

In order to avoid interference between the holder 21 and the opening of the buffer chamber 11 and between the holder 21 and the opening of the waste liquid chamber 12, the second direction in which the holder 21 and the cartridge 1 are stacked is also perpendicular to the third direction. As shown in fig. 2, the fixing frame 21 may be fixed to a plate member surrounding the buffer chamber 11 and the waste liquid chamber 12 by screwing, welding, or the like. The lifting table 22 is slidably connected to the fixing frame 21 and can slide along the second direction relative to the fixing frame 21. In a possible implementation manner, as shown in fig. 2, a guide rail extending along the second direction is provided on the fixing frame 21, and the fixing frame 21 is sleeved on the guide rail to realize sliding connection between the lifting platform 22 and the fixing frame 21. The driving force for sliding the lift table 22 in the second direction may be manually driven by an operator, driven by a mechanical driving mechanism, or the like.

The lifting table 22 can slide in the second direction and also stay at two set positions, i.e., a first height and a second height in the second direction. With the lift table 22 at the first height, each pipetting needle 3 is withdrawn from the buffer cassette for the operator to remove the buffer cassette or to insert the buffer cassette. When the lifting table 22 is at the second height, each pipetting needle 3 enters the buffer box and reaches a set depth in the buffer box so that the gene sequencer can withdraw the buffer through the pipetting needle 3. Specifically, the manner in which the lifting platform 22 stays at the first height and the second height may include, but is not limited to, providing a buckle at the first height and the second height on the fixing frame 21, being held by an electric device or a hydraulic device, and the like.

The number of the liquid sucking needles 3 is at least one, and each liquid sucking needle 3 is fixed on one side of the lifting table 22 facing the buffer box along the first direction. Each of the pipette needles 3 can be lifted and lowered along with the lifting table 22 while the lifting table 22 slides along the second height. One end of each pipetting needle 3 is in communication with a buffer tube of the gene sequencer for withdrawing buffer and delivering to a desired location during operation of the gene sequencer.

In general, different types of buffers are needed in different sequencing processes of a gene sequencer, so that multiple buffers can be stored separately in a buffer box, and each liquid sucking needle 3 corresponds to one buffer so as to satisfy that different types of buffers can be extracted at different stages in the sequencing process. The number of the pipetting needles 3 being at least one means that the number of the pipetting needles 3 may be one, two, three or more. The specific number of buffer needles may be determined according to the number of types of buffer actually required. As an example, a liquid extraction and collection device as illustrated in fig. 2 is provided with 3 buffer needles.

The waste liquid pipe connector 4 is fixed to the lifting table 22 toward the waste liquid chamber 12 side in the first direction, and the waste liquid pipe connector 4 is used for communicating a waste liquid pipe of the gene sequencer and guiding waste liquid generated by the gene sequencer to the waste liquid chamber 12. Specifically, referring to fig. 2, the waste pipe connector 4 may be fixedly connected to the lifting table 22 by screwing, welding, or the like. The waste pipe connector 4 can also be lifted and lowered by sliding along the lifting table 22. With the lift table 22 at the first height, the waste pipe connector 4 may enter the waste box or proximate to the box mouth of the waste box to direct waste into the waste box. When the lifting table 22 is at the first height, the waste pipe connector 4 is a waste box, so that an operator can conveniently take out the waste box.

The liquid extracting and collecting device integrates the waste liquid chamber 12 and the buffer liquid chamber 11 in the liquid storage box 1, can simultaneously accommodate the waste liquid box and the buffer liquid box, simultaneously sets the liquid suction needle 3 and the waste liquid pipe communicating part at two sides of the lifting table 22, realizes the integrated design of the buffer liquid storage device, the buffer liquid extracting device and the waste liquid collecting device, has a compact structure, saves the internal space of the gene sequencer, and is favorable for the miniaturized design of the gene sequencer.

In addition, after the lifting table 22 is lifted to the second height, the liquid suction needle 3 is withdrawn from the buffer box, the waste liquid pipe connecting piece 4 is also far away from the waste liquid box, the buffer box and the waste liquid box can be replaced simultaneously, the waste liquid pipe connecting piece 4 and the liquid suction needle 3 do not need to be operated one by one, the operation flow of replacing the liquid storage box 1 is simplified, and the operation convenience of the gene sequencer is improved.

When the lifting table 22 is driven to lift by the driving mechanism, the driving device 23 can drive the lifting table 22 to slide between the first height and the second height along the second direction. The driving mechanism can adopt different driving modes. The drive means 23 may, for example, be in the form of an electric motor, hydraulic means, pneumatic means, etc. The control program of the driving means 23 may be integrated in the gene sequencer. By the control of the control module, when the waste liquid box or the buffer liquid box needs to be replaced, the driving device 23 drives the lifting table 22 to be lifted from the second height to the first height; after the replacement of the waste liquid box or the setting of the buffer liquid box is completed, the driving device 23 drives the lifting table 22 to be lowered from the first height to the second height.

The lifting table 22 is driven to lift by the driving device 23, so that manual operation can be saved, meanwhile, a control program can be integrated in the gene sequencer, more intelligent automatic operation is realized, and the operation convenience of the gene sequencer is improved.

As an alternative embodiment, a safety device may be further disposed on the fixing frame 21, where the safety device is used to limit the sliding track range of the lifting platform 22 between the first height and the second height, prevent the lifting platform 22 from exceeding the range between the first height and the second height, and further reduce the risk of damaging the lifting device 2 due to the excessive lifting amplitude of the lifting platform 22.

Optionally, the safety device includes a first safety stopper and a second safety stopper, both of which are fixed on the fixing frame 21. The first safety limiting block meets the requirement that if the lifting table 22 has a continuous lifting trend after being lifted to the first height, the first limiting block can prop against the lifting table 22 to limit the lifting table 22 to continuously lift; the second limiting block can prop against the lifting table 22 to limit the lifting table 22 to continuously descend if the lifting table 22 has a continuous descending trend after descending to the second height. The physical limit can be realized by adopting the form of the limit block, the limit aging possibility is lower, and the reliability is better.

The construction of a possible cartridge 1 is described in detail below with reference to the accompanying drawings. Referring to fig. 3, fig. 3 is a schematic diagram of a liquid storage box according to an embodiment of the application.

As an alternative embodiment, the cartridge 1 further comprises a middle partition 13, the middle partition 13 being fixed inside the cartridge 1 and separating the waste liquid chamber 12 from the buffer liquid chamber 11. The middle partition plate 13 is adopted to separate the waste liquid chamber 12 from the buffer liquid chamber 11, so that the separation is easy to realize in production and manufacture, and meanwhile, a good separation effect can be achieved, and the middle partition plate 13 is adopted to directly physically separate the waste liquid chamber 12 from the buffer liquid chamber 11, so that the risk of polluting reagents in the buffer liquid chamber 11 with waste liquid of the waste liquid chamber 12 can be reduced.

Of course, in some other possible embodiments, the separation of the waste liquid chamber 12 from the buffer chamber 11 may also be achieved by providing ribs or the like on the plate material surrounding the cartridge 1 instead of the intermediate partition 13. The plate can be saved by adopting the form of incomplete physical separation such as convex ribs, and the weight of the liquid storage box 1 is reduced, so that the effect of light weight is achieved. However, the use of ribs may increase the risk of contamination of the buffer, and in particular, the manner in which the buffer is used may be determined by comprehensive evaluation.

As an alternative embodiment, a stopper plunger 14 may be provided in the waste liquid chamber 12, in the buffer liquid chamber 11, and on the intermediate partition 13. As shown in fig. 3, the limiting plunger 14 is arranged on the plate material surrounding the liquid storage box 1 and the middle partition plate 13 in a penetrating way. The limiting plunger 14 is used for abutting with the buffer solution box and the waste solution box and limiting the buffer solution box to be located at a first set position in the buffer solution chamber 11 and limiting the waste solution box to be located at a second set position in the waste solution chamber 12. The direction in which the stopper plunger 14 abuts against the waste liquid cartridge is a first direction and a third direction, and the first setting position and the second setting position are both positions of orthogonal planes in the first direction and the second direction. Adopt spacing plunger 14 restriction buffer box and waste liquid box in the position on the orthogonal plane of first direction and second direction for when placing buffer box and waste liquid box, the operator only need concentrate on in the third direction with waste liquid box mouth aim at waste liquid pipe connecting piece 4 and with buffer box mouth to imbibition needle 3, and then reduced the degree of difficulty of placing buffer box and waste liquid box, improved the convenience of operation.

In particular, the specific form of the limiting plunger 14 may employ a spring plunger or the like to reduce the risk of jamming of the limiting plunger 14 with the buffer or waste cartridge when the buffer and waste cartridge are removed or placed.

As an alternative embodiment, the buffer chamber 11 is provided with guide holes 15 on the side facing the lifting table 22, the number of the guide holes 15 is the same as that of the liquid sucking needles 3, and the positions of the guide holes 15 are opposite to those of the liquid sucking needles 3 one by one along the second direction. When the lifting table 22 slides in the second direction, each pipetting needle 3 can be inserted into or withdrawn from the buffer chamber 11 through the corresponding guide hole 15. Specifically, the guide hole 15 is formed in a plate material having the buffer chamber 11 facing the lifting table 22 in the second direction. By providing a series of guide holes 15, each of the pipetting needles 3 can be guided to precisely enter a corresponding region in the buffer box during descent along the second direction, and the risk of the pipetting needle 3 deviating from a predetermined position is reduced.

Alternatively, the guide hole 15 may be in the form of a tapered hole in the form of a gradually decreasing diameter of the guide hole 15 in the direction from the lifting table 22 to the cartridge 1 in the second direction. In the form of a tapered hole, the taper of the tapered hole may be used to correct the pipette needle 3 to a predetermined position when the pipette needle 3 is offset from the predetermined position by a predetermined distance.

As an alternative embodiment, a recognition device 16 is also provided in the buffer chamber 11. The identifying means 16 is used for identifying information carried by the buffer cassette, such as identifying information of the kind of buffer in the buffer cassette, information of the number of buffers, information of the production of the buffers, etc., when the buffer cassette is placed in the buffer chamber 11.

Different types of identification devices 16 may be employed when specifically configured. For example, the identification device 16 may be a radio frequency identification (RFID, radio Frequency Identification) device to read information carried by an electronic tag on the buffer cassette; the identification means 16 may also be a photoelectric identification device to read two-dimensional codes, bar code information, etc. on the buffer cartridge.

By providing the identification means 16 in the buffer chamber 11, it is ensured that the buffer cassette placed in the buffer chamber 11 is a correct buffer cassette, reducing the influence of erroneous use of the buffer on the sequencing result and even damaging the sequencing equipment. Meanwhile, the identification equipment can be connected into a laboratory inventory management system, so that efficient management of experimental resources and the like can be realized.

Some possible arrangements of the waste pipe connector 4 are described in detail below with reference to the accompanying drawings. Referring to fig. 4, fig. 4 is a schematic diagram of a waste pipe connector according to an embodiment of the present application. First, to improve the gene detection efficiency, a gene sequencer generally adopts a multi-channel design, that is, a plurality of waste liquid pipes may be connected to a plurality of sequencing channels in a gene sequencer.

As an alternative embodiment, the waste pipe connector 4 comprises a connecting disc 41 fixedly connected to the lifting table 22, at least one waste pipe hole 42 being provided on the connecting disc 41, each waste pipe hole 42 being adapted to communicate with one waste pipe. Referring to fig. 4, an exemplary structure in which a connection plate is fixed to the side of the elevating table 22 facing the waste liquid chamber 12, and a connection plate 41 is fixedly connected to the side of the connection plate facing the waste liquid chamber 12 through two connection posts, realizes the fixation of the connection plate 41 to the elevating table 22. The connection pad 41 illustrated in fig. 4 is provided with four waste-liquid-pipe connection holes, that is, the waste-liquid-pipe connection pad 41 can simultaneously connect 4 waste liquid pipes. Of course, the number of the waste liquid pipe connecting holes may be 1, 2, 3, 5, etc. according to the number of the actual waste liquid pipes.

The connection disc 41 can slide along the lifting table 22 to lift along the second direction, meanwhile, the waste liquid chamber 12 is provided with a through hole 17 towards one side of the lifting table 22, and when the lifting table 22 is lowered to the second height, the connection disc 41 can pass through the through hole 17 to be attached to the box mouth of the waste liquid box. Specifically, when the elevating table 22 is lowered to the second height, the connection plate 41 may enter the box opening of the waste liquid box so as to guide all the waste liquid into the waste liquid box; meanwhile, the connecting disc 41 forms a certain cover on the box mouth of the waste liquid box, and can prevent waste liquid from splashing after entering the waste liquid box to a certain extent. When the lifting table 22 is lifted to the first height, the connecting disc 41 is withdrawn from the waste liquid box opening, so that personnel can conveniently take out or put in the waste liquid box.

As an example, the connection disc 41 as shown in fig. 4 is a disc, which is to match a circular cartridge opening of the waste cartridge. In other possible implementations, if the waste cartridge port is of other shapes, the connecting disc 41 may be of other shapes, such as oval, rectangular, etc.

The connecting disc 41 fixed with the lifting table 22 is adopted to guide the waste liquid into the waste liquid box, so that on one hand, the waste liquid box has better guiding effect and waste liquid splashing preventing effect; on the other hand, when the waste liquid box is full of waste liquid, the lifting table 22 can be used for lifting the waste liquid pipe connecting disc 41 to withdraw from the box opening of the waste liquid box, so that the replacement operation of the waste liquid box is facilitated.

As an alternative embodiment, the waste pipe connection 4 is also provided with a liquid level detection means 43; the liquid level detecting device 43 is opposed to the opening of the waste liquid cartridge and detects the liquid level in the waste liquid cartridge. Referring to fig. 4, an exemplary liquid level detecting device 43 is fixed to a central portion of the connection pad 41, and the liquid level detecting device 43 may be lifted up and down along with the lifting of the lifting table 22. When the lifting table 22 is at the second height, the detection head of the liquid level detection device 43 can enter the waste liquid box opening or be close to the box opening of the waste liquid box, and detect the height of the waste liquid in the waste liquid box. The detection result of the waste liquid detection device can be displayed in real time, and a prompt and the like can be sent to an operator when the waste liquid box is full or about to be full. Alternatively, the specific liquid level detection means 43 may be a photoelectric liquid level sensor, an ultrasonic liquid level sensor, a radar liquid level sensor, or the like.

By providing the liquid level detection device 43 in the waste liquid pipe connector 4, the liquid level in the waste liquid box can be detected, and the operator can grasp the information of the liquid level in the waste liquid box. Operators do not need to observe the liquid pressing surface height of the waste liquid box in real time, the convenience of the gene sequencer is improved, and the risk of overflow of waste liquid caused by full filling of the waste liquid box is reduced.

It should be understood that the buffer box in this embodiment may be replaced by a buffer bottle or a buffer test tube in practical implementation; likewise, the waste liquid box can also be replaced by a container such as a waste liquid bottle.

The embodiment of the application also provides a gene sequencer, which comprises at least one buffer liquid pipe, at least one waste liquid pipe and any liquid extraction and collection device. Wherein, at least one buffer liquid pipe and imbibition needle one-to-one intercommunication, at least one waste liquid pipe and waste liquid tube hole one-to-one intercommunication.

The present disclosure is intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Any omissions, modifications, equivalents, improvements, and the like, which are within the spirit and principles of the one or more embodiments of the disclosure, are therefore intended to be included within the scope of the disclosure.

The foregoing is merely illustrative embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily think about variations or substitutions within the technical scope of the present application, and the application should be covered. Therefore, the protection scope of the application is subject to the protection scope of the claims.

Claims

1.A liquid extraction and collection device for use in a gene sequencer comprising:

2. The liquid extraction and collection device of claim 1, wherein the cartridge further comprises a middle baffle; the middle partition separates the waste liquid chamber from the buffer liquid chamber.

3. The liquid extraction and collection device of claim 2, wherein limiting plungers are disposed in the waste liquid chamber, in the buffer chamber, and on the intermediate partition;

4. The liquid extracting and collecting device as claimed in claim 1, wherein a guide hole is provided at a side of the buffer chamber facing the elevating table;

5. The liquid extraction and collection device according to claim 1, wherein the buffer chamber is provided with identification means for identifying identification information of the buffer cartridge.

6. The liquid extraction and collection device of claim 1, wherein the waste tube connector comprises a connecting disc fixedly connected to the lifting platform; at least one waste liquid pipe hole is formed in the connecting disc, and each waste liquid pipe hole is communicated with one waste liquid pipe;

7. The liquid extraction and collection apparatus of claim 6 wherein said waste pipe connection is further provided with liquid level detection means;

8. The liquid extraction and collection device of any one of claims 1 to 7, wherein the liquid extraction and collection device further comprises a drive device;

9. The liquid extraction and collection device of claim 8, wherein a safety device is provided on the mount;

10. A genetic sequencer comprising at least one buffer tube, at least one waste tube, and a liquid extraction and collection device according to any one of claims 1 to 9; wherein: