CN221100774U - Full-automatic western blotting instrument - Google Patents
Full-automatic western blotting instrument Download PDFInfo
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- CN221100774U CN221100774U CN202322893636.0U CN202322893636U CN221100774U CN 221100774 U CN221100774 U CN 221100774U CN 202322893636 U CN202322893636 U CN 202322893636U CN 221100774 U CN221100774 U CN 221100774U
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- 238000001262 western blot Methods 0.000 title claims abstract description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 45
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 22
- 230000001360 synchronised effect Effects 0.000 claims abstract description 22
- 239000002699 waste material Substances 0.000 claims description 14
- 238000004140 cleaning Methods 0.000 abstract description 5
- 238000012864 cross contamination Methods 0.000 abstract description 4
- 238000011534 incubation Methods 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 2
- 239000012459 cleaning agent Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model relates to the field of medical detection, in particular to a full-automatic western blotting instrument, which comprises an instrument body, wherein a working cavity is arranged at one side in the instrument body, a fixed platform, a swinging platform and a driving mechanism are arranged at the back side of the fixed platform in the sliding platform instrument body at equal intervals in the working cavity; the driving mechanism comprises a first driving motor, a first driving shaft, a sliding bearing, a limiting sleeve, a second driving motor, a second driving shaft, a synchronous pulley and a synchronous belt, and a first centrifugal reagent tube and a second centrifugal reagent tube. According to the utility model, the automatic closed incubation and cleaning of the liquid of the western blot analyzer and the recovery of the solution reagents are realized through the driving mechanism, the touch control screen, the swinging platform, the sliding platform, the liquid outlet pipe, the collecting mechanism, the instrument door and the instrument body, the working steps in the whole working flow are operated one by one, and cross contamination is not easy to occur between the reagent solutions.
Description
Technical Field
The utility model relates to the technical field of medical detection, in particular to a full-automatic western blotting instrument.
Background
Western Blot (immunoblot assay) is known as Western Blot. It is a common experimental method in molecular biology, biochemistry and immunogenetics. The basic principle is that a cell or biological tissue sample treated by gel electrophoresis is stained by a specific antibody. Information on the expression of a specific protein in the analyzed cell or tissue is obtained by analyzing the position of staining and the depth of staining.
In the actual use process of the existing Western blotting instrument, equipment in the instrument is required to be manually cleaned, cross contamination is easily caused to a reagent solution in the cleaning process, and the cleaning process is complicated.
Disclosure of utility model
The utility model aims to solve the defects in the prior art, and provides a full-automatic western blotting instrument.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
A full-automatic western blot instrument comprises an instrument body, wherein a working cavity is arranged on one side in the instrument body, a fixed platform, a swinging platform and a sliding platform are arranged in the working cavity at equal intervals, liquid outlet pipes are symmetrically arranged in the middle of the top of the fixed platform, and a driving mechanism is arranged on the back side, positioned on the fixed platform, in the instrument body;
The driving mechanism comprises a first driving motor, a first driving shaft, a sliding bearing, a limiting sleeve, a second driving motor, a second driving shaft, a synchronous pulley and a synchronous belt, and a first centrifugal reagent tube and a second centrifugal reagent tube.
In addition, the preferred structure is, fixed platform's top is located the equal symmetry in both sides of corresponding drain pipe and is equipped with first funnel, and fixed platform's below is equipped with swing platform, and swing platform's top is located the first funnel department that corresponds and all is equipped with the working groove.
In addition, the preferred structure is, swing platform's below is equipped with slide platform, and slide platform's top is equipped with two sets of collection mechanism, and collection mechanism includes first collection box, second funnel, and the second funnel is placed at slide platform's top, and slide platform's top is located the left side of second funnel and is equipped with first collection box, and the right side of second funnel is equipped with the second and collects the box.
In addition, the preferable structure is that the lower part of the sliding platform is provided with a waste liquid groove, and a waste liquid groove is arranged in the waste liquid groove.
In addition, the preferred structure is, first driving motor's output is connected with first drive shaft, and the middle part cover of stop collar's outer wall is equipped with slide bearing, and slide bearing's outer wall cover is equipped with the stop collar.
In addition, it is preferable that a second driving motor is arranged below the first driving motor, and a second driving shaft is connected to an output end of the second driving motor.
In addition, the preferable structure is that the middle parts of the outer walls of the second driving shaft and the first driving shaft are respectively sleeved with a synchronous pulley, and a synchronous belt is sleeved between the outer walls of the two synchronous pulleys.
In addition, the preferred structure is, the outer wall of first drive shaft just is located slide bearing's both sides and all installs first centrifugal reagent pipe, and the outer wall of stop collar just is located synchronous pulley's both sides and all installs the second centrifugal reagent pipe.
In addition, the preferred structure is, the preceding terminal surface of instrument body just is located one side of working chamber and installs touch control screen, and touch control screen passes through electric signal connection control first driving motor, second driving motor, swing platform, sliding platform.
The beneficial effects of the utility model are as follows:
According to the utility model, the automatic closed incubation, cleaning and recovery of the liquid of the western blot analyzer are realized through the driving mechanism, the touch control screen, the swinging platform, the sliding platform, the liquid outlet pipe, the collecting mechanism, the instrument door and the instrument body 1, and in the whole working flow, the working steps are operated one by one, and cross contamination is not easy to generate between reagent solutions.
Drawings
FIG. 1 is a schematic diagram of a fully automated Western blotting instrument according to the present utility model;
FIG. 2 is a schematic diagram showing the structure of a second centrifuge reagent tube of a full-automatic Western blotting apparatus according to the present utility model;
Fig. 3 is a schematic structural diagram of a driving mechanism of a full-automatic western blotting instrument according to the present utility model.
In the figure: 1 instrument body, 11 touch control screen, 21 fixed platform, 22 swing platform, 23 sliding platform, 3 actuating mechanism, 31 first actuating motor, 311 first drive shaft, 312 slide bearing, 313 stop collar, 32 second actuating motor, 321 second drive shaft, 33 synchronous pulley, 331 hold-in range, 41 first centrifugal reagent pipe, 42 second centrifugal reagent pipe, 43 drain pipe, 5 first funnel, 6 working grooves, 71 first collection box, 72 second collection box, 8 second funnel, 9 waste slag groove.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Referring to fig. 1-3, a full-automatic western blotting instrument comprises an instrument body 1, wherein a working cavity is arranged on one side in the instrument body 1, a fixed platform 21, a swinging platform 22 and a sliding platform 23 are equidistantly arranged in the working cavity, liquid outlet pipes 43 are symmetrically arranged in the middle of the top of the fixed platform 21, and a driving mechanism 3 is arranged on the back side of the fixed platform 21 in the instrument body 1;
The driving mechanism 3 includes a first driving motor 31, a first driving shaft 311, a sliding bearing 312, a spacer 313, a second driving motor 32, a second driving shaft 321, a timing pulley 33, and a timing belt 331, and a first centrifuge reagent tube 41 and a second centrifuge reagent tube 42.
Wherein, the top of fixed platform 21 is located the equal symmetry of both sides of corresponding drain pipe 43 and is equipped with first funnel 5, and the below of fixed platform 21 is equipped with swing platform 22, and the top of swing platform 22 is located the first funnel 5 department that corresponds and all is equipped with work groove 6, through swing platform 22, can realize sending out the swing to work groove 6 by a short margin to when making the reagent solution in the work groove 6 mix evenly, make the reagent solution after the misce bene in the work groove 6 empty, the follow-up operation of being convenient for.
Wherein, swing platform 22's below is equipped with slide platform 23, slide platform 23's top is equipped with two sets of collection mechanism, collection mechanism includes first collection box 71, second collection box 72, second funnel 8 is placed at slide platform 23's top, slide platform 23's top is located the left side of second funnel 8 and is equipped with first collection box 71, second funnel 8's right side is equipped with second collection box 72, the waste liquid recess has been seted up to slide platform 23's below, be equipped with waste liquid groove 9 in the waste liquid recess, collect the first reagent solution in the first centrifugation reagent pipe 41 through first collection box 71, collect the second reagent solution in the second centrifugation reagent pipe 42 through second collection box 72, collect the cleaner that goes out drain pipe 43 and guide to waste residue groove 9 through second funnel 8, and concentrate through waste residue groove 9 finally.
Meanwhile, the output end of the first driving motor 31 is connected with a first driving shaft 311, a sliding bearing 312 is sleeved in the middle of the outer wall of the first driving shaft 311, and a limit sleeve 313 is sleeved on the outer wall of the sliding bearing 312.
And, the below of first driving motor 31 is equipped with second driving motor 32, and the output of second driving motor 32 is connected with second drive shaft 321, and the middle part of the outer wall of second drive shaft 321, stop collar 313 all overlaps and is equipped with synchronous pulley 33, and the cover is equipped with hold-in range 331 between two synchronous pulley 33 outer walls.
Further, the first centrifugal reagent tube 41 is mounted on both sides of the outer wall of the first driving shaft 311 and the sliding bearing 312, and the second centrifugal reagent tube 42 is mounted on both sides of the outer wall of the stop collar 313 and the synchronous pulley 33.
Further, the touch control screen 11 is installed on the front end face of the instrument body 1 and located on one side of the working cavity, the touch control screen 11 is connected with and controls the first driving motor 31, the second driving motor 32, the swing platform 22 and the sliding platform 23 through electric signals, and the instrument door is installed on the top of the instrument body 1 and located above the corresponding working cavity in a rotating mode.
In the present embodiment, the first reagent solution is injected into the first centrifuge reagent tube 41, the second reagent solution is injected into the second centrifuge reagent tube 42, and then the instrument door is turned and closed, thereby closing the working chamber of the main body 1.
Then, the first driving motor 31 and the swinging platform 22 are controlled to be opened through the touch control screen 11, wherein the corresponding first driving shaft 311 is driven to rotate towards the first funnel 5 through the first driving motor 31, so that the first centrifugal reagent tube 41 rotates towards the first funnel 5, the first reagent solution in the first centrifugal reagent tube 41 is poured into the first funnel 5 and is guided towards the working tank 6 through the first funnel 5, then the working tank 6 can be swung slightly through the swinging platform 22, so that the first reagent solution in the working tank 6 is uniformly mixed, meanwhile, the sliding platform 23 is controlled to be opened through the touch control screen 11, the first collecting box 71 at the top of the sliding platform 23 is aligned with the working tank 6, then the uniformly mixed first reagent solution in the working tank 6 is poured into the first collecting box 71 through the swinging platform 22, and the first reagent solution is collected through the first collecting box 71.
Then, the cleaning agent is injected into the liquid outlet pipe 43, flows to the first funnel 5 through the liquid outlet pipe 43, flows to the working tank 6 through the first funnel 5, shakes the cleaning agent in the working tank 6 through the swinging platform 22, and cleans the working tank 6, meanwhile, the sliding platform 23 is controlled to be opened through the touch control screen 11, the second funnel 8 at the top of the sliding platform 23 is aligned to the working tank 6, then the cleaning agent in the working tank 6 is poured into the second funnel 8 through the swinging platform 22, and the cleaning agent flowing out of the liquid outlet pipe 43 is collected and guided to the waste slag tank 9 through the second funnel 8, and finally is concentrated through the waste slag tank 9.
Then, the second driving motor 32 and the swinging platform 22 are controlled to be opened through the touch control screen 11, wherein the second driving motor 32 drives the corresponding second driving shaft 321 to rotate towards the first funnel 5, as the second driving shaft 321 and the middle part of the outer wall of the limiting sleeve 313 are respectively sleeved with the synchronous belt pulley 33, and the synchronous belts 331 are sleeved between the outer walls of the two synchronous belt pulleys 33, the second centrifugal reagent pipes 42 are respectively arranged on the outer walls of the limiting sleeve 313 and the two sides of the synchronous belt pulley 33, so that the second driving shaft 321 drives the corresponding limiting sleeve 313 and the second centrifugal reagent pipes 42 to rotate towards the first funnel 5 while rotating towards the first funnel 5, and then the second reagent solution box in the second centrifugal reagent pipes 42 is poured into the first funnel 5, and guided towards the working tank 6 through the first funnel 5, then the swinging platform 22 can realize small-amplitude swinging of the working tank 6, thereby enabling the first reagent solution in the working tank 6 to be uniformly mixed, and meanwhile, the sliding platform 23 is controlled to be opened through the touch control screen 11, and the second collecting box 72 at the top of the sliding platform 23 is enabled to be aligned with the second collecting box 72 through the second hopper 6, and then the second reagent solution is uniformly poured into the second collecting box 72 through the second hopper 6.
According to the utility model, the automatic closed incubation and cleaning of the liquid of the western blot analyzer and the recovery of the solution reagents are realized through the driving mechanism 3, the touch control screen 11, the swinging platform 22, the sliding platform 23, the liquid outlet pipe 43, the collecting mechanism, the instrument door and the instrument body 1, and in the whole working flow, the working steps are operated one by one, and the cross contamination is not easy to occur between the reagent solutions.
The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.
Claims (9)
1. The full-automatic western blot instrument comprises an instrument body (1) and is characterized in that a working cavity is formed in one side in the instrument body (1), a fixed platform (21), a swinging platform (22) and a sliding platform (23) are arranged in the working cavity at equal intervals, liquid outlet pipes (43) are symmetrically arranged in the middle of the top of the fixed platform (21), and a driving mechanism (3) is arranged on the back side of the fixed platform (21) in the instrument body (1);
The driving mechanism (3) comprises a first driving motor (31), a first driving shaft (311), a sliding bearing (312), a limiting sleeve (313), a second driving motor (32), a second driving shaft (321), a synchronous pulley (33) and a synchronous belt (331), and a first centrifugal reagent tube (41) and a second centrifugal reagent tube (42).
2. The full-automatic western blotting instrument according to claim 1, wherein the top of the fixed platform (21) is located at two sides of the corresponding liquid outlet pipe (43) and is symmetrically provided with a first funnel (5), the lower part of the fixed platform (21) is provided with a swinging platform (22), and the top of the swinging platform (22) is located at the corresponding first funnel (5) and is provided with a working groove (6).
3. The full-automatic western blotting instrument according to claim 2, wherein a sliding platform (23) is arranged below the swinging platform (22), two groups of collecting mechanisms are arranged at the top of the sliding platform (23), each collecting mechanism comprises a first collecting box (71), a second collecting box (72) and a second funnel (8), the second funnel (8) is arranged at the top of the sliding platform (23), a first collecting box (71) is arranged at the top of the sliding platform (23) and is positioned at the left side of the second funnel (8), and a second collecting box (72) is arranged at the right side of the second funnel (8).
4. A fully automated western blotting apparatus according to claim 3, wherein a waste liquid groove is provided below the sliding platform (23), and a waste liquid groove (9) is provided in the waste liquid groove.
5. The full-automatic western blotting instrument according to claim 1, wherein the output end of the first driving motor (31) is connected with a first driving shaft (311), a sliding bearing (312) is sleeved in the middle of the outer wall of the first driving shaft (311), and a limit sleeve (313) is sleeved on the outer wall of the sliding bearing (312).
6. The full-automatic western blotting instrument according to claim 5, wherein a second driving motor (32) is arranged below the first driving motor (31), and an output end of the second driving motor (32) is connected with a second driving shaft (321).
7. The full-automatic western blotting instrument according to claim 6, wherein the outer walls of the second driving shaft (321) and the limiting sleeve (313) are respectively sleeved with a synchronous pulley (33), and a synchronous belt (331) is sleeved between the outer walls of the two synchronous pulleys (33).
8. The full-automatic western blotting apparatus according to claim 7, wherein the first centrifuge reagent tube (41) is installed on both sides of the outer wall of the first driving shaft (311) and the sliding bearing (312), and the second centrifuge reagent tube (42) is installed on both sides of the outer wall of the stop collar (313) and the synchronous pulley (33).
9. The full-automatic western blotting instrument according to claim 1, wherein a touch control screen (11) is installed on one side of the working cavity on the front end surface of the instrument body (1), and the touch control screen (11) is connected with and controls the first driving motor (31), the second driving motor (32), the swinging platform (22) and the sliding platform (23) through electric signals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322893636.0U CN221100774U (en) | 2023-10-27 | 2023-10-27 | Full-automatic western blotting instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322893636.0U CN221100774U (en) | 2023-10-27 | 2023-10-27 | Full-automatic western blotting instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221100774U true CN221100774U (en) | 2024-06-07 |
Family
ID=91315647
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322893636.0U Active CN221100774U (en) | 2023-10-27 | 2023-10-27 | Full-automatic western blotting instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221100774U (en) |
-
2023
- 2023-10-27 CN CN202322893636.0U patent/CN221100774U/en active Active
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