CN221078483U - Diagnostic detection equipment for assay box - Google Patents

Abstract

The utility model relates to the technical field of virus detection, and particularly discloses diagnostic detection equipment for an assay box, which is used for assisting the diagnostic detection of virus types by the assay box with a specific structure through the cooperation of an objective table component, a liquid distribution control mechanism, a reaction control mechanism and a reaction shooting mechanism, so that the efficiency of the diagnostic detection of the viruses can be accelerated.

Description

Diagnostic detection equipment for assay box

Technical Field

The utility model relates to the technical field of virus detection, in particular to diagnostic detection equipment of an assay box.

Background

The existing virus detection technology is very mature, can detect various viruses, but most of the existing virus detection devices are not disposable articles, and the virus detection devices need to be thoroughly cleaned and disinfected after each use, so that a great deal of working time is consumed.

For solving the above-mentioned problem, provided a disposable virus detection device (namely an assay box for detecting virus type) that is portable and can detect fast in the trade, see a disposable virus detection device that chinese patent 2022102042164 discloses, including piston element, main casing sealing membrane, main casing sealing element, AMP amplification chamber sealing membrane and AMP runner component, piston element bottom slidable mounting has the main casing sealing membrane, the piston element bottom is provided with the main casing, main casing bottom slidable mounting has the main casing sealing element, main casing sealing element bottom is provided with AMP amplification chamber sealing element, AMP amplification chamber sealing element bottom is provided with AMP amplification chamber sealing membrane, be provided with AMP runner component between main casing top end fixed mounting has main cap, main casing side fixed mounting has detection device, main casing bottom slidable mounting has controlling means, controlling means and main casing sealing element slidable mounting have main casing sealing element, main casing sealing element bottom is provided with AMP amplification chamber sealing element, AMP amplification chamber sealing element bottom is provided with AMP amplification chamber sealing element, AMP amplification device rotates runner device with AMP amplification chamber sealing element. Compared with the existing virus detection device, the disposable virus detection device can rapidly and stably detect internal virus stock solution during use, so that the whole detection mode is simpler, the convenience of the virus detection device during use is greatly improved, the accuracy of final detection data is ensured, the working quality of the virus detection device is greatly improved, the use effect of the whole part is ensured, and the positions of the internal part and the stock solution can be detected and controlled, so that the device is more convenient during use, the situation that error occurs in the detection data of the virus detection device due to the existence of other factors is avoided, and the virus detection device is stably assisted in detection; and because the cover virus detection device is a disposable article, the cover virus detection device does not need to consume manpower to clean and disinfect, and saves working time.

However, the virus detection apparatus disclosed in the above chinese patent 2022102042164 requires the auxiliary use of a diagnostic detection device to obtain results, and thus, it is necessary in the industry to provide a device for assisting the diagnostic detection of viruses by the above virus detection apparatus.

Disclosure of utility model

The present utility model is directed to solving the technical problems of the prior art described above and providing a diagnostic test device for an assay cartridge.

In order to solve the technical problems, the utility model provides the following technical scheme:

The utility model relates to a diagnosis and detection device of an assay box, which comprises an objective table component, a liquid preparation control mechanism, a reaction control mechanism and a reaction shooting mechanism; the objective table assembly, the liquid preparation control mechanism, the reaction control mechanism and the reaction shooting mechanism are all connected in the mounting frame;

The stage assembly includes a stage body coupled to the mounting frame for carrying the assay cartridge; the test box is arranged at the test box mounting through hole;

The liquid preparation control mechanism comprises a piston control assembly and a valve rotating assembly which are connected to the mounting frame; the piston control assembly comprises a liftable piston clamping piece, the valve rotating assembly is positioned on one side of the piston control assembly, and the valve rotating assembly comprises a liftable valve rotating piece; the piston clamping piece and the valve rotating piece are both positioned above the objective table component, and the valve rotating piece is positioned below the piston clamping piece;

The reaction control mechanism comprises a first heating component which is connected to the mounting frame and can elastically lift on the mounting frame, the first heating component comprises a first heat conduction block, and the first heat conduction block is positioned below the objective table component and is used for heating the mixed liquid on the detection chip in the assay box;

The reaction shooting mechanism comprises a shooting piece, wherein the shooting piece is positioned above the objective table component and is used for shooting an imaging result after the mixed liquid on the detection chip in the assay box reacts.

According to the test box diagnosis and detection equipment, a test box is arranged at a test box installation through hole on a test box body in a test box assembly, so that a piston element on the test box is clamped by a piston clamping piece in a liquid preparation control mechanism, the piston element is clamped by the piston clamping piece through lifting so as to adapt to different heights of the piston element, and meanwhile, an external virus stock solution in the piston element is injected into a fluid storage chamber through the piston clamping piece so as to mix the external virus stock solution with liquid in the fluid storage chamber; the control valve on the assay box is clamped by the valve rotating part in the liquid preparation control mechanism, and the control valve on the assay box is driven to rotate by the valve rotating part, so that one fluid storage chamber on the assay box is communicated with the sample storage chamber, the mixed liquid in the fluid storage chamber flows into the sample storage chamber, flows out through the fluid outlet and is then conveyed to a detection chip in the detection device; meanwhile, when the test box is arranged at the test box installation through hole on the object stage body, the bottom of the test box corresponding to the installation position of the detection chip on the test box is contacted with the first heat conduction block in the reaction control mechanism, the detection chip on the test box is heated through the first heat conduction block so as to promote the reaction of the mixed liquid on the detection chip, after the reaction is finished, the imaging result after the reaction is shot through the shooting part in the reaction shooting mechanism, the shot content is transmitted to the computer terminal, and the virus type is judged through software arranged on the computer terminal. The diagnosis and detection device of the assay box assists a disposable virus detection device (namely the disposable assay box) in Chinese patent 2022102042164 to carry out diagnosis and detection of virus types, so that the efficiency of virus diagnosis and detection can be accelerated.

Further, the piston control assembly comprises a first screw rod and a first sliding seat capable of lifting on the first screw rod, an internal thread is arranged in the first sliding seat, the first screw rod vertically penetrates through the first sliding seat, and an external thread on the first screw rod is matched with the internal thread in the first sliding seat; the piston clamping piece is connected to the first sliding seat.

Further, a second sliding seat is connected to the first sliding seat, a second screw rod vertically penetrates through the second sliding seat, an internal thread is arranged in the second sliding seat, an external thread on the second screw rod is matched with the internal thread in the second sliding seat, and the second sliding seat is promoted to lift on the second screw rod through rotation of the second screw rod; the piston clamping piece is connected to the second sliding seat.

Further, the piston clamping piece is an electric claw or an air claw.

Further, the valve rotating assembly comprises a third screw rod and a third sliding seat capable of lifting on the third screw rod, an internal thread is arranged in the third sliding seat, the third screw rod vertically penetrates through the third sliding seat, and an external thread on the third screw rod is matched with the internal thread in the third sliding seat; the valve rotating piece is connected to the third sliding seat.

Further, a mounting seat which rises and falls along with the third sliding seat is arranged on the third sliding seat, a driving gear and a driven gear are arranged on the mounting seat, the driving gear is connected with a driving mechanism for driving the third sliding seat to rotate positively and negatively, the driving gear is driven to rotate positively and negatively through the driving mechanism, and the driving gear drives the driven gear to rotate positively and negatively; the driven gear vertically penetrates through the valve rotating piece, and the driven gear is used for driving the valve rotating piece to rotate positively and negatively.

Further, the valve rotating member is a sleeve.

Further, a clearance gear is arranged between the driving gear and the driven gear, and the clearance gear is meshed with the driving gear and the driven gear.

Further, connect the chemical examination box and transfer the casting die in the mount pad bottom, the chemical examination box transfers the casting die including being the groove type that the level set up and transfer the casting die, is provided with the elasticity pressing piece that is used for pushing down the chemical examination box on transfer the both sides wall of casting die respectively.

Further, a grating sensor for detecting the position of the control valve in the cartridge is also provided at the side of the valve rotating assembly.

Further, the first heating component further comprises a first peltier element, the first peltier element is arranged at the bottom of the first heat conducting block, a heat dissipation aluminum block is further arranged at the bottom of the first peltier element, and a first air supply piece is arranged on the heat dissipation aluminum block.

Further, the reaction control mechanism further comprises a second heating component arranged on one side of the first heating component, the second heating component comprises a second heat conduction block, a second Peltier element is arranged at the bottom of the second heat conduction block, a heat exchange water tank is further arranged at the bottom of the second Peltier element, a water inlet pipe and a water outlet pipe are communicated with the heat exchange water tank, the water inlet pipe and the water outlet pipe are further communicated with a heat exchange piece, the heat exchange piece comprises a mounting frame, a plurality of water channels are arranged on the mounting frame at intervals, water outlets and water inlets of adjacent water channels are communicated to form a meandering heat dissipation flow channel, the water inlet of the heat dissipation flow channel is communicated with the outlet of the water outlet pipe, and the water outlet of the heat dissipation flow channel is communicated with the inlet of the water inlet pipe.

Furthermore, a wavy heat dissipation aluminum piece is arranged between the adjacent water channels;

and/or a second air supply piece for supplying air to the water channel is arranged on the mounting frame.

Further, the reaction control mechanism comprises an ultrasonic vibrator for oscillating the mounting position of the detection chip in the assay box;

and/or the reaction control mechanism further comprises a waveform generator for electrically connecting with a detection chip in the assay cartridge.

Further, the shooting piece comprises a camera and a coaxial light path module connected with a camera lens.

Further, the reaction shooting mechanism further comprises a vertical lifting assembly, the vertical lifting assembly comprises a fourth screw rod which is vertically arranged and a fourth sliding seat which is lifted on the fourth screw rod, an internal thread is arranged in the fourth sliding seat, the fourth screw rod vertically penetrates through the fourth sliding seat, and an external thread on the fourth screw rod is matched with the internal thread in the fourth sliding seat; the shooting piece is connected to the fourth sliding seat.

Further, a horizontal moving assembly is connected to the fourth sliding seat, the horizontal moving assembly comprises two horizontal sliding seats connected in the vertical direction, the horizontal sliding seat positioned below is connected to the fourth sliding seat in a reciprocating movement manner, the horizontal sliding seat positioned above is connected to a sixth sliding seat, and the shooting piece is connected to the sixth sliding seat; the moving directions of the two horizontal sliding seats are vertical, and the horizontal sliding seat positioned above can reciprocate relative to the horizontal sliding seat positioned below the horizontal sliding seats; each horizontal sliding seat is internally provided with an internal thread, two horizontal movement transmission screw rods respectively penetrate through one horizontal sliding seat, the external thread of each horizontal movement transmission screw rod is matched with the internal thread of the corresponding horizontal sliding seat, and the horizontal movement transmission screw rods are positively and negatively rotated to promote the horizontal sliding seats to reciprocate.

Further, the objective table assembly further comprises two fifth sliding seats for driving the objective table body to reciprocate along the direction approaching or separating from the liquid preparation control mechanism, a sliding rail is arranged below each fifth sliding seat, and each fifth sliding seat is connected to the corresponding sliding rail and reciprocates relative to the sliding rail; the fifth sliding seat is horizontally penetrated with a fifth screw rod, an internal thread is arranged in the fifth sliding seat, an external thread on the fifth screw rod is matched with an internal thread in the corresponding fifth sliding seat, and the fifth sliding seat is driven to reciprocate on the corresponding sliding rail by forward and backward rotation of the fifth screw rod.

Further, a first elastic supporting component is arranged between the object stage body and the fifth sliding seats, the first elastic supporting component comprises mounting plates arranged on each fifth sliding seat, at least one first support column is vertically arranged on each mounting plate, the object stage body is sleeved on each first support column and can lift along the first support column, a first spring is sleeved on each first support column, and the first spring is positioned between the object stage body and the corresponding mounting plate;

And/or a plurality of second elastic supporting components which enable the reaction control mechanism to elastically lift are arranged below the object stage body.

Drawings

The foregoing and other objects, features and advantages of the utility model will be apparent from the following more particular description of preferred embodiments of the utility model, as illustrated in the accompanying drawings. Like reference numerals refer to like parts throughout the drawings, and the drawings are not intentionally drawn to scale on actual size or the like, with emphasis on illustrating the principles of the utility model.

FIG. 1 is a schematic diagram of the structure of the present utility model.

Fig. 2 and 3 are schematic structural views of the liquid dispensing control mechanism.

Fig. 4 is a schematic diagram of a connection structure of a driving gear, a clearance gear and a driven gear.

Fig. 5 is a partial cross-sectional view of a channel-type hold-down.

FIG. 6 is a schematic structural view of the reaction control mechanism.

Fig. 7 is a schematic view of a heat exchange member.

Fig. 8 is a schematic diagram of the structure of the reaction shooting mechanism.

Wherein, objective table subassembly: a stage body 101 of (a); a fifth slide seat 102; a slide rail 103; a fifth screw 104; a mounting plate 105; a first strut 106; a first spring 107; a second leg 108; a second spring 109;

Liquid preparation control mechanism: a piston clamp 201; a first screw 202; a first sliding seat 203; a second sliding seat 204; a second screw 205; a third screw 206; a third slide block 207; a valve rotation member 208; a mounting base 209; a drive gear 210; a driven gear 211; a clearance gear 212; a groove-shaped pressing portion 213; a pressing portion 214; pressing the spring 215; a baffle 216; a grating sensor 217;

Reaction control mechanism: a first support plate 301; a first thermally conductive block 302; a first peltier element 303; a heat-dissipating aluminum block 304; a first air supply member 305; a second support plate 306; a second heat conduction block 307; a second peltier element 308; a heat exchange water tank 309; a waterway 312; a wave-shaped heat radiation aluminum member 313; a second air supply member 314; an ultrasonic vibrator 315; a waveform generator 316;

Reaction shooting mechanism: a camera 401; a coaxial optical path module 402; a fourth slide seat 403; a horizontal sliding seat 404; a sixth sliding seat 405; a horizontal movement transfer screw 406;

Assay cartridge 50;

Mounting frame 60: supporting leg 601.

Detailed Description

In order that the utility model may be understood more fully, the utility model will be described with reference to the accompanying drawings.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to and integrated with the other element or intervening elements may also be present. The terms "mounted," "one end," "the other end," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used in the description herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

The utility model provides a concrete implementation mode of a test box diagnosis and detection device, which is mainly used for assisting a disposable virus detection device disclosed in China patent 2022102042164 to carry out diagnosis and detection of virus types; so as to accelerate the efficiency and accuracy of virus diagnosis and detection. Wherein, a disposable virus detection device disclosed in China patent 2022102042164 is the assay cartridge of the utility model.

In this embodiment, referring to fig. 1, the cartridge diagnostic test device includes a stage assembly, a liquid dispensing control mechanism, a reaction control mechanism, and a reaction photographing mechanism; the objective table component is used for bearing the test box 50 and providing the contact condition of the test box 50 with the reaction control mechanism and the liquid preparation control mechanism; the liquid preparation control mechanism is used for controlling the lifting of a piston element in the assay box 50 and controlling the rotation of a control valve in the device; the reaction control mechanism is used for heating the mixed liquid (the mixed liquid is the mixture of the external virus stock solution and the sample extracting solution) on the detection chip in the assay box 50 and the mixed liquid stored in the main shell, and promoting the mixed liquid to be uniformly distributed on the surface of the chip; the reaction shooting mechanism is used for shooting an imaging result after the mixed liquid on the detection chip reacts, and can send the shot imaging result to the computer terminal, and the virus type is judged through software installed on the computer terminal.

In this embodiment, referring to fig. 2 and 3, the liquid dispensing control mechanism includes a piston control assembly and a valve rotating assembly connected to a mounting frame; the piston control assembly comprises a liftable piston clamping piece 201, the piston control assembly further comprises a first screw rod 202 and a first sliding seat 203 which can be lifted on the first screw rod 202, internal threads are arranged in the first sliding seat 203, namely, through holes are directly formed in the first sliding seat 203, internal threads are formed in the inner walls of the through holes, the first screw rod 202 vertically penetrates through the first sliding seat 203, external threads on the first screw rod 202 are matched with the internal threads in the first sliding seat 203, through holes can also be formed in the first sliding seat 203, nuts are arranged in the through holes, and when the first screw rod 202 penetrates through the first sliding seat 203, the nuts need to be screwed in at the same time, so that the external threads on the first screw rod 202 are matched with the internal threads of the nuts; the piston clamp 201 is connected to a first slide mount 203.

In the preferred embodiment, referring to fig. 2 and 3, a second sliding seat 204 is connected to the first sliding seat 203, a second screw rod 205 is vertically penetrating through the second sliding seat 204, and an internal thread is provided inside the second sliding seat 204, that is, a through hole is directly provided inside the second sliding seat 204, an internal thread is provided on an inner wall of the through hole, the second screw rod 205 vertically penetrates through the second sliding seat 204, an external thread on the second screw rod 205 is matched with an internal thread inside the second sliding seat 204, or a through hole is provided inside the second sliding seat 204, a nut is provided in the through hole, and when the second screw rod 205 penetrates through the second sliding seat 204, the nut is screwed into the second screw rod 205 at the same time, so that the external thread on the second screw rod 205 is matched with the internal thread of the nut; the second sliding seat 204 is promoted to lift on the second screw rod 205 by the rotation of the second screw rod 205; in the case of the second slide seat 204, the piston clamping member 201 is directly connected to the second slide seat 204, i.e. the piston clamping member 201 is indirectly connected to the first slide seat 203.

In this embodiment, referring to fig. 2 and 3, the piston clamping member 201 may be an electric claw or an air claw, and the electric claw is preferably selected, so that the air claw needs to provide an air source, which increases the volume of the device, and the electric claw is selected, which only needs to provide a power source, so that the volume of the device can be reduced.

In this embodiment, referring to fig. 2 and 3, the valve rotating assembly is located at one side of the piston control assembly, the valve rotating assembly includes a third screw rod 206 and a third sliding seat 207 capable of lifting on the third screw rod 206, the inside of the third sliding seat 207 is provided with internal threads, that is, a through hole is directly provided in the inside of the third sliding seat 207, an internal thread is provided on the inner wall of the through hole, the third screw rod 206 vertically penetrates through the third sliding seat 207, an external thread on the third screw rod 206 is matched with an internal thread in the inside of the third sliding seat 207, or a through hole is provided in the inside of the third sliding seat 207, a nut is provided in the through hole, and when the third screw rod 206 penetrates through the third sliding seat 207, the nut is screwed in at the same time, so that the external thread on the third screw rod 206 is matched with the internal thread of the nut; the valve rotating assembly comprises a valve rotating member 208, and the valve rotating member 208 is connected to the third sliding seat 207 and rises and falls along with the third sliding seat 207; the piston clamp 201 is located above the valve rotation member 208 and the valve rotation member 208 is located above the stage assembly.

In this embodiment, referring to fig. 2-4, a mounting seat 209 that ascends and descends along with the third sliding seat 207 is provided, a driving gear 210 and a driven gear 211 are provided on the mounting seat 209, the driving gear 210 is connected with a driving mechanism that drives the third sliding seat to rotate in a forward and reverse direction, the driving gear 210 is driven by the driving mechanism to rotate in a forward and reverse direction, and the driving gear 210 drives the driven gear 211 to rotate in a forward and reverse direction; the driven gear 211 vertically penetrates through the valve rotating member 208, and the driven gear 211 rotates forward and backward to drive the valve rotating member 208 to rotate forward and backward. The driven gear 211 comprises a gear body and a mounting hole arranged on the gear body, the valve rotating piece 208 penetrates through the mounting hole, the valve rotating piece 208 is a sleeve, the sleeve and the mounting hole on the gear body can be connected in an interference fit manner, or an external thread is arranged on the outer side wall of the sleeve, an internal thread is arranged on the inner wall of the mounting hole on the gear body, and the sleeve is installed in the mounting hole through gear engagement, so that the sleeve is driven to rotate through the rotation of the driven gear 211; the shape of the sleeve matches the shape of the control valve of the control device in cartridge 50 so that the sleeve can fit over the control valve and rotate the control valve.

In a preferred embodiment, referring to fig. 4, a clearance gear 212 is disposed between the driving gear 210 and the driven gear 211, the clearance gear 212 is meshed with both the driving gear 210 and the driven gear 211, and the driving gear 210 rotates to drive the clearance gear 212 to rotate, so that the driven gear 211 is driven to rotate by the clearance gear 212. By providing a clearance gear 212, the valve rotating assembly is prevented from interfering with adjacent other assemblies or parts.

In a preferred embodiment, referring to fig. 4, a test cartridge pressing member is connected to the bottom of the mounting seat 209, and the test cartridge pressing member includes a groove-shaped pressing portion 213 horizontally arranged, and since the pressing portion is in a groove shape, when both side walls of the pressing portion are pressed against the test cartridge 50, the pressing portion can avoid the main casing of the test cartridge 50, avoid interference, press the test cartridge 50 through the groove-shaped pressing portion 213, and enable the test cartridge 50 to be in contact with a reaction control mechanism below, and meanwhile avoid shaking of the test cartridge 50, so as to ensure that the placement state of the test cartridge 50 is stable; referring to fig. 5, elastic pressing members for pressing down the cartridge 50 are respectively provided on both sidewalls of the groove-shaped pressing portion 213, and when the groove-shaped pressing portion 213 presses down the cartridge 50, the elastic pressing members elastically contact the surface of the cartridge 50, thereby preventing the cartridge 50 from being damaged due to direct rigid contact; the elastic pressing piece comprises a pressing part 214 which is arranged in a through hole of the side wall of the groove-shaped pressing part 213 and can stretch elastically, a pressing spring 215 is arranged in the through hole, a baffle 216 is arranged at the top end of the pressing spring 215 and at the port of the through hole, one end of the pressing spring 215 is propped against the baffle 216, the other end of the pressing spring 215 is propped against the pressing part 214, and part of the pressing part 214 penetrates out of the corresponding port of the through hole under the elastic action of the pressing spring 215 so as to be convenient for contacting with the assay box 50, and the pressing part 214 can be made of materials such as rubber or can be directly wrapped with a layer of soft materials on the pressing part 214, so that the assay box 50 is prevented from being damaged by the pressing part 214.

In a preferred embodiment, see fig. 2 and 3, a grating sensor 217 for detecting the position of the control valve in the cartridge 50 is also provided on the side of the valve rotating assembly, which grating sensor 217 may also be called grating position sensor. Since the control valve of the control device in the assay box 50 is provided with a plurality of grating notches at equal intervals, the main casing of the assay box 50 is provided with a grating window, the grating notch on the control valve can be seen through the grating window, the grating sensor 217 is opposite to the grating window on the assay box 50, the grating sensor 217 is opposite to the grating notch on the control valve through the grating window, and the rotation position of the control valve can be detected by the grating sensor 217 according to the working principle of the grating sensor 217 in the rotation process of the control valve, so that the fluid flow channel on the control valve is communicated with any fluid storage chamber in the main casing.

In this embodiment, referring to fig. 6, the reaction control mechanism includes a first heating member connected to the mounting frame and elastically liftable on the mounting frame, the first heating member being disposed under the stage body 101 in the stage member, the first heating member including a first support plate 301 and a first heat-conducting block 302, a through hole being provided in the first support plate 301, the first heat-conducting block 302 being disposed at the through hole, the first heat-conducting block 302 being partially located under the first support plate 301, partially penetrating the through hole in the first support plate 301 and then protruding above the first support plate 301, a portion of the first heat-conducting block 302 protruding above the first support plate 301 being for contacting with a position of a chip mounting groove of the assay cartridge 50 to heat a detection chip mounted in the chip mounting groove; the first heat-conducting block 302 is located under the stage body 101 in the stage assembly and is opposite to the mounting through hole on the stage body 101, and when the cartridge 50 is placed in the mounting through hole of the stage body 101 and in a detection diagnosis state, the chip mounting groove thereon is in contact with the first heat-conducting block 302 to heat the mixed liquid on the detection chip in the cartridge 50.

In this embodiment, referring to fig. 6, the first heating component further includes a first peltier element 303, where the first peltier element 303 is disposed at the bottom of the first heat conducting block 302, and belongs to the prior art, and it may generate thermoelectric effect, that is, thermoelectric refrigeration and heating phenomena, and by disposing the first peltier element 303, two temperatures in the two ranges, that is, a first temperature range 58-62 ℃ and a second temperature range 53-57 ℃, may be provided in the present utility model, which is because the detection chip in the assay cartridge belongs to a biochip, in biotechnology, a process of capturing RNA and a process of capturing a reaction between a target and nanogold need to be performed in the first temperature range, such as 60 ℃, and a process of performing a reaction between a subsequent nanosilver and a target with nanogold need to be performed in the second temperature range, such as 55 ℃, and by the function specific to the first peltier element 303 itself, the switching between the two temperatures may be rapidly implemented, so that not only the accuracy of the result of the mixed solution after the reaction on the detection chip is ensured, but also the diagnostic efficiency of the detection is improved; because the first peltier element 303 generates heat during the working process, in order to ensure the normal operation of the device, a heat dissipation aluminum block 304 is further disposed at the bottom of the first peltier element 303, and a first air supply member 305 is disposed on the heat dissipation aluminum block 304 to accelerate the heat dissipation speed; the first air supply member 305 may be a fan.

In this embodiment, referring to fig. 6, the reaction control mechanism further includes a second heating component disposed at one side of the first heating component, the second heating component includes a second support plate 306 and a second heat conducting block 307, a through hole is disposed on the second support plate 306, the second heat conducting block 307 is disposed at the through hole, a part of the second heat conducting block 307 is located below the second support plate 306, and extends to above the second support plate 306 after passing through the through hole on the second support plate 306, and a part of the second heat conducting block 307 extending to above the second support plate 306 is used for contacting with the bottom of the main casing of the assay cartridge 50 so as to heat the mixed liquid in the main casing; the second heat-conducting block 307 is located under the stage body 101 in the stage assembly and is opposite to the mounting through hole on the stage body 101, and when the cartridge 50 is placed in the mounting through hole of the stage body 101 and is in a detection diagnosis state, the bottom of the main casing thereon is in contact with the second heat-conducting block 307 to heat the mixed liquid stored in the main casing in the cartridge 50.

In a preferred embodiment, referring to fig. 6, a second peltier element 308 is disposed at the bottom of the second heat conducting block 307, and by providing the second peltier element 308, three ranges of temperatures can be provided in the present utility model, namely, a first temperature range of 50-60 ℃, a second temperature range of 70-75 ℃ and a third temperature range of 93-97 ℃, which are due to the three temperature ranges required for the polymerase chain reaction (i.e., PCR) process in biotechnology, and by the unique function of the second peltier element 308 itself, three temperature switching can be rapidly achieved, thereby improving the efficiency of detection and diagnosis; because the second peltier element 308 generates heat during the working process, in order to ensure the normal operation of the device, a heat exchange water tank 309 is further arranged at the bottom of the second peltier element 308, a water inlet pipe and a water outlet pipe are communicated with the heat exchange water tank 309, the water inlet pipe and the water outlet pipe are also communicated with a heat exchange member, and referring to fig. 7, the heat exchange member comprises a mounting bracket, a plurality of water channels 312 are arranged on the mounting bracket at intervals, water outlets and water inlets of adjacent water channels 312 are communicated to form a tortuous heat dissipation runner, the water inlet of the heat dissipation runner is communicated with the outlet of the water outlet pipe, and the water outlet of the heat dissipation runner is communicated with the inlet of the water inlet pipe; the heat dissipation is performed by circulating water.

The heat conducting soft cushions are arranged on the surfaces of the first heat conducting block 302 and the second heat conducting block 307, the thickness of the heat conducting soft cushions is not more than 1.5mm, the heat conductivity is more than 10W/(m.times.K), and the heat conducting soft cushions are arranged, so that the damage to the test box caused by the heat conducting blocks can be avoided, and meanwhile, the normal heat conduction can be ensured.

In a preferred embodiment, referring to fig. 7, a wave-shaped heat dissipation aluminum member 313 is disposed between adjacent water channels 312 to absorb heat of circulating water in the water channels 312, thereby realizing heat dissipation; a second air supply piece 314 for supplying air to the water channel 312 is arranged on the mounting bracket, and the air cooling and the water cooling are combined to further and rapidly radiate the heat in the circulating water; the second air supply member 314 may be a fan.

In a preferred embodiment, referring to fig. 6, the reaction control mechanism further includes an ultrasonic vibrator 315, where the ultrasonic vibrator 315 is disposed on the first support plate 301, and when the test cartridge 50 is placed in the mounting through hole of the stage body 101 and is in a detection diagnosis state, the ultrasonic vibrator 315 contacts with a housing corresponding to the chip mounting slot of the test cartridge 50, and periodic oscillation is generated by the ultrasonic vibrator 315, and the oscillation frequency can be set in a range of 20KHz-50KHz, so that the mixed solution on the detection chip in the test cartridge 50 is uniformly distributed on the surface of the detection chip, so as to improve the detection accuracy.

In a preferred embodiment, referring to FIG. 6, the reaction control mechanism further comprises a waveform generator 316 for electrical connection with the detection chip in the cartridge 50. Specifically, the interface on the detection chip is only connected with the interface of the waveform generator 316 through a wire. The wave generator 316 generates a periodic electric field, the frequency of the periodic electric field can be set at 10Hz-500KHz, and the output voltage amplitude can be set at 0V-10V, so that the mixed liquid on the detection chip is further uniformly distributed on the surface of the detection chip, and the detection accuracy is improved.

In this embodiment, referring to fig. 8, the reaction photographing mechanism includes a photographing member located above the stage assembly for photographing the imaging result after the reaction of the mixed solution on the chip in the assay cartridge 50; specifically, the detection device of the test cartridge 50 has a light-transmitting window opposite to the detection chip, and when the test cartridge 50 is placed at the mounting through hole on the stage body 101 and is in the detection state, the photographing member faces the light-transmitting window on the test cartridge 50. The shooting piece comprises a camera 401 and a coaxial light path module 402 connected with a camera 401 lens, wherein the coaxial light path module 402 can be connected to the camera 401 lens in a clamping manner, so that the coaxial light path module 402 is opposite to a light transmission window on the assay box 50, the coaxial light path module 402 belongs to the prior art, transmission, focusing and reflection of light rays can be ensured through the cooperation of the coaxial light path module 402 and the camera 401, clear images or visual fields can be obtained, and an imaging result after the mixed liquid uploaded to a computer terminal is reacted can be accurately judged.

In a preferred embodiment, referring to fig. 8, the reaction shooting mechanism further includes a vertical lifting assembly, where the vertical lifting assembly includes a fourth screw rod (not shown in the figure) that is vertically disposed and a fourth sliding seat 403 that is lifted on the fourth screw rod, an internal thread is disposed inside the fourth sliding seat 403, that is, a through hole is directly disposed inside the fourth sliding seat 403, an internal thread is disposed on an inner wall of the through hole, the fourth screw rod vertically penetrates through the fourth sliding seat 403, an external thread on the fourth screw rod is matched with the internal thread inside the fourth sliding seat 403, or a through hole is disposed inside the fourth sliding seat 403, a nut is disposed in the through hole, and when the fourth screw rod penetrates through the fourth sliding seat 403, the nut needs to be screwed in at the same time, so that the external thread on the fourth screw rod is matched with the internal thread of the nut; the photographing member is coupled to the fourth slide holder 403. In addition, the driving mechanism for driving the fourth screw rod to rotate can be directly connected with the fourth screw rod, or the output shaft of the driving mechanism is in transmission connection with the fourth screw rod through a synchronous belt and a synchronous belt wheel. The vertical height of the photographing member relative to the detecting chip on the testing cartridge 50 is adjusted by the cooperation of the fourth screw and the fourth sliding seat 403.

In a preferred embodiment, referring to fig. 8, a horizontal moving assembly is connected to the fourth sliding seat 403, the horizontal moving assembly includes two horizontal sliding seats 404 connected in a vertical direction, the lower horizontal sliding seat 404 is reciprocally movably connected to the fourth sliding seat 403, the upper horizontal sliding seat 404 is reciprocally movably connected to the lower horizontal sliding seat 404, and the upper horizontal sliding seat 404 is further connected to a sixth sliding seat 405 moving along with the upper horizontal sliding seat, and the photographing element is connected to the sixth sliding seat 405; the moving directions of the two horizontal sliding seats 404 are perpendicular, i.e. the moving direction of one horizontal sliding seat 404 is along the direction of the coordinate axis Y, and the moving direction of the other horizontal sliding seat 404 is along the direction of the coordinate axis X; each horizontal sliding seat 404 is internally provided with an internal thread, namely a through hole is directly formed in the horizontal sliding seat 404, the internal thread is formed in the inner wall of the through hole, the horizontal movement transmission screw rod 406 vertically penetrates through the horizontal sliding seat 404, the external thread on the horizontal movement transmission screw rod 406 is matched with the internal thread in the horizontal sliding seat 404, a through hole is also formed in the horizontal sliding seat 404, a nut is arranged in the through hole, and when the horizontal movement transmission screw rod 406 penetrates through the horizontal sliding seat 404, the nut is required to be screwed in at the same time, so that the external thread on the horizontal movement transmission screw rod 406 is matched with the internal thread of the nut, and the horizontal sliding seat 404 is driven to reciprocate by forward and backward rotation of the horizontal movement transmission screw rod 406.

The corresponding horizontal sliding seat 404 is driven to move along the corresponding direction by any horizontal movement of the transmission screw rod 406, so that the shooting piece can be focused, and when the focusing stroke is exceeded, the shooting piece is driven to move in a vertical direction in a compensating way by the transmission of the fourth screw rod, so that the shooting piece can be focused smoothly, and the definition of a shot picture or a visual field is ensured.

Referring to fig. 1, in this embodiment, the stage assembly includes a stage body 101 for carrying the cartridge 50; the stage body 101 is provided with a cartridge mounting through hole, the shape of which is adapted to the contour shape of the cartridge 50, and the cartridge 50 is mounted at the cartridge mounting through hole.

In a preferred embodiment, referring to fig. 1, since the placement and the removal of the assay cartridge 50 require manual operation, the stage body 101 needs to be able to withdraw from the detection position, so as to facilitate the manual removal of the assay cartridge 50, and specifically, the stage assembly further includes two fifth sliding seats 102 that drive the stage body 101 to reciprocate in a direction approaching or separating from the liquid dispensing control mechanism, a sliding rail 103 is disposed below each fifth sliding seat 102, and each fifth sliding seat 102 is connected to the corresponding sliding rail 103 and reciprocates relative to the sliding rail 103; the fifth screw rod 104 horizontally penetrates through at least one fifth sliding seat 102, an internal thread is arranged in the fifth sliding seat 102, namely a through hole is directly formed in the fifth sliding seat 102, an internal thread is arranged on the inner wall of the through hole, the fifth screw rod 104 vertically penetrates through the fifth sliding seat 102, an external thread on the fifth screw rod 104 is matched with the internal thread in the fifth sliding seat 102, or a through hole is formed in the fifth sliding seat 102, a nut is arranged in the through hole, and when the fifth screw rod 104 penetrates through the fifth sliding seat 102, the nut is required to be screwed in at the same time, so that the external thread on the fifth screw rod 104 is matched with the internal thread of the nut; the fifth screw rod 104 is rotated forward and backward to drive the fifth sliding seat 102 to reciprocate on the corresponding sliding rail 103, so that the fifth sliding seat 102 drives the objective table body 101 to reciprocate.

In a preferred embodiment, referring to fig. 1, a first elastic support assembly is disposed between the stage body 101 and the fifth sliding seat 102, the first elastic support assembly includes a mounting plate 105 mounted on each fifth sliding seat 102, at least one first strut 106 is vertically disposed on the mounting plate 105, in this embodiment, two first struts 106 are disposed on each fifth sliding seat 102 at intervals, the stage body 101 is sleeved on the first struts 106 and can be lifted along the first struts 106, a first spring 107 is sleeved on the first struts 106, and the first spring 107 is located between the stage body 101 and the corresponding mounting plate 105; when the test cartridge 50 moves to the detection position along with the test cartridge body 101 (i.e. moves to the lower part of the reaction control mechanism and the liquid preparation control mechanism), the groove-shaped pressing part 213 in the liquid preparation control mechanism can actively press the test cartridge 50, so that the bottom of the test cartridge 50 is contacted with the first heat conducting block 302 and the second heat conducting block 307 in the reaction control mechanism, the corresponding area of the test cartridge 50 is heated, and as the test cartridge body 101 can elastically lift on the first support column 106, when the test cartridge 50 is pressed down, the test cartridge body 101 is pushed to move downwards at the same time, after the detection is finished, the groove-shaped pressing part 213 is lifted and reset, the test cartridge 50 is not pressed down any more, at the moment, the test cartridge body 101 is not stressed any more, and then the bottom of the test cartridge 50 is driven to lift and reset under the elastic restoring action of the first spring 107, the first heat conducting block 302 and the second heat conducting block 307 in the reaction control mechanism, and then the test cartridge 50 is driven to move the test cartridge 50 and leave the detection position manually by the fifth slide seat 102 and the fifth screw 104.

In a preferred embodiment, referring to fig. 1, a plurality of second elastic supporting components for elastically lifting and lowering the reaction control mechanism are further provided under the stage body 101. The plurality of second elastic support components are arranged around the first heating component and the second heating component of the reaction control mechanism, the second elastic support components comprise second support columns 108, the second support columns 108 comprise bottom columns and top columns connected to the top ends of the bottom columns, the top column sizes are smaller than the bottom column sizes, so that a first step surface exists at the joint of the bottom columns and the top columns, second springs 109 are sleeved on the top columns, a first support plate 301 and a second support plate 306 in the reaction control mechanism are sleeved on the top columns of the adjacent second elastic support components through connecting lug plates respectively, the first support plate 301, the first heat conduction block 302, the first peltier element 303, the first air supply piece 305 and the heat dissipation aluminum block 304 in the first heating component are in a suspended state, the second support plate 306, the second heat conduction block 307, the second peltier element 308 and the heat exchange water tank 309 in the second heating component in an integrated state, when the assay box 50 is pressed down to be abutted against the first heat conduction block 302 and the second heat conduction block 307, the first support plate 302 and the second heat conduction block 307 are also driven to be in a suspended state, and the assay box 50 is prevented from being damaged by the buffer box 50 and the second heat conduction block 307 and the second heat conduction block 302 and the second heat conduction block 307 and the second heat conduction block 304 in the integrated state, and the buffer block is prevented from being damaged by the buffer box 50; after the assay cartridge 50 no longer gives pressure to the first and second heat conducting blocks 302 and 307, the first and second support plates 301 and 306 are urged to return to their mounted parts under the elastic restoring action of the second spring 109, respectively.

In this embodiment, the above driving mechanism for driving the first screw 202, the second screw 205, the third screw 206, the fourth screw, the fifth screw 104, and the horizontal movement transmission screw 406 to rotate may be a mechanism capable of outputting a rotational movement by a motor or the like that is capable of rotating in the forward and reverse directions.

In this embodiment, referring to fig. 1, the above liquid dispensing control mechanism, reaction shooting mechanism and stage assembly are all laid out and connected in a mounting frame 60, and a supporting leg 601 is further provided at the bottom of the mounting frame 60 to suspend the whole assay cartridge diagnostic and detection apparatus, so as to facilitate the whole movement of the assay cartridge diagnostic and detection apparatus.

In the present application, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

In the description of the present specification, a description of the terms "preferred embodiment," "further embodiment," "other embodiments," or "specific examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

While embodiments of the present application have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the application, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the application.

Claims (11)

1. An assay cassette diagnostic test device, characterized by: comprises an objective table component, a liquid preparation control mechanism, a reaction control mechanism and a reaction shooting mechanism; the objective table assembly, the liquid preparation control mechanism, the reaction control mechanism and the reaction shooting mechanism are all connected in the mounting frame;

The stage assembly includes a stage body coupled to the mounting frame for carrying the assay cartridge; the test box is arranged at the test box mounting through hole;

The liquid preparation control mechanism comprises a piston control assembly and a valve rotating assembly which are connected to the mounting frame; the piston control assembly comprises a liftable piston clamping piece, the valve rotating assembly is positioned on one side of the piston control assembly, and the valve rotating assembly comprises a liftable valve rotating piece; the piston clamping piece and the valve rotating piece are both positioned above the objective table component, and the valve rotating piece is positioned below the piston clamping piece;

The reaction control mechanism comprises a first heating component which is connected to the mounting frame and can elastically lift on the mounting frame, the first heating component comprises a first heat conduction block, and the first heat conduction block is positioned below the objective table component and is used for heating the mixed liquid on the detection chip in the assay box;

The reaction shooting mechanism comprises a shooting piece, wherein the shooting piece is positioned above the objective table component and is used for shooting an imaging result after the mixed liquid on the detection chip in the assay box reacts.

2. The cartridge diagnostic test device of claim 1 wherein: the piston control assembly comprises a first screw rod and a first sliding seat capable of lifting on the first screw rod, wherein an internal thread is arranged in the first sliding seat, the first screw rod vertically penetrates through the first sliding seat, and an external thread on the first screw rod is matched with the internal thread in the first sliding seat; the piston clamping piece is connected to the first sliding seat;

And/or the valve rotating assembly comprises a third screw rod and a third sliding seat which can be lifted on the third screw rod, wherein an internal thread is arranged in the third sliding seat, the third screw rod vertically penetrates through the third sliding seat, and an external thread on the third screw rod is matched with the internal thread in the third sliding seat; the valve rotating piece is connected to the third sliding seat;

And/or a grating sensor for detecting the position of the control valve in the cartridge is also provided laterally of the valve rotation assembly.

3. The cartridge diagnostic test device of claim 2 wherein: the first sliding seat is connected with a second sliding seat, a second screw rod vertically penetrates through the second sliding seat, an internal thread is arranged in the second sliding seat, an external thread on the second screw rod is matched with the internal thread in the second sliding seat, and the second sliding seat is promoted to lift on the second screw rod through the rotation of the second screw rod; the piston clamping piece is connected to the second sliding seat;

And/or a mounting seat which rises and falls along with the third sliding seat is arranged on the third sliding seat, a driving gear and a driven gear are arranged on the mounting seat, the driving gear is connected with a driving mechanism for driving the third sliding seat to rotate positively and negatively, the driving gear is driven to rotate positively and negatively through the driving mechanism, and the driving gear drives the driven gear to rotate positively and negatively; the driven gear vertically penetrates through the valve rotating piece, and the driven gear is used for driving the valve rotating piece to rotate positively and negatively.

4. The cartridge diagnostic test device of claim 3 wherein: a clearance gear is arranged between the driving gear and the driven gear, and the clearance gear is meshed with the driving gear and the driven gear;

And/or connect the test box and push down the piece in mount pad bottom, test box pushes down the piece and includes being the groove type that the level set up pushes down the portion, is provided with the elasticity pressing piece that is used for pushing down the test box on pushing down the both sides wall of portion respectively.

5. The cartridge diagnostic test device of any one of claims 1-4 wherein: the first heating component further comprises a first Peltier element, the first Peltier element is arranged at the bottom of the first heat conduction block, a heat dissipation aluminum block is further arranged at the bottom of the first Peltier element, and a first air supply piece is arranged on the heat dissipation aluminum block;

And/or the reaction control mechanism comprises an ultrasonic vibrator for oscillating the mounting position of the detection chip in the assay box;

and/or the reaction control mechanism further comprises a waveform generator for electrically connecting with a detection chip in the assay cartridge.

6. The cartridge diagnostic test device of claim 5, wherein: the reaction control mechanism further comprises a second heating component arranged on one side of the first heating component, the second heating component comprises a second heat conduction block, a second Peltier element is arranged at the bottom of the second heat conduction block, a heat exchange water tank is further arranged at the bottom of the second Peltier element, a water inlet pipe and a water outlet pipe are communicated with the heat exchange water tank, the water inlet pipe and the water outlet pipe are further communicated with a heat exchange piece, the heat exchange piece comprises a mounting frame, a plurality of water channels are arranged on the mounting frame at intervals, water outlets and water inlets of adjacent water channels are communicated with each other to form a meandering heat dissipation flow channel, the water inlets of the heat dissipation flow channel are communicated with the outlet of the water outlet pipe, and the water outlets of the heat dissipation flow channel are communicated with the inlet of the water inlet pipe.

7. The cartridge diagnostic test device of claim 6 wherein: wave-shaped heat dissipation aluminum pieces are arranged between adjacent water channels;

and/or a second air supply piece for supplying air to the water channel is arranged on the mounting frame.

8. The cartridge diagnostic test device of any one of claims 1-4 or 6-7, wherein: the shooting piece comprises a camera and a coaxial light path module connected with a camera lens;

And/or the reaction shooting mechanism further comprises a vertical lifting assembly, wherein the vertical lifting assembly comprises a fourth screw rod which is vertically arranged and a fourth sliding seat which is lifted on the fourth screw rod, an internal thread is arranged in the fourth sliding seat, the fourth screw rod vertically penetrates through the fourth sliding seat, and an external thread on the fourth screw rod is matched with the internal thread in the fourth sliding seat; the shooting piece is connected to the fourth sliding seat.

9. The cartridge diagnostic test device of claim 8 wherein: the horizontal moving assembly comprises two horizontal sliding seats connected in the vertical direction, the lower horizontal sliding seat is connected to the fourth sliding seat in a reciprocating manner, the upper horizontal sliding seat is connected with a sixth sliding seat, and the shooting piece is connected to the sixth sliding seat; the moving directions of the two horizontal sliding seats are vertical, and the horizontal sliding seat positioned above can reciprocate relative to the horizontal sliding seat positioned below the horizontal sliding seats; each horizontal sliding seat is internally provided with an internal thread, two horizontal movement transmission screw rods respectively penetrate through one horizontal sliding seat, the external thread of each horizontal movement transmission screw rod is matched with the internal thread of the corresponding horizontal sliding seat, and the horizontal movement transmission screw rods are positively and negatively rotated to promote the horizontal sliding seats to reciprocate.

10. The cartridge diagnostic test device of any one of claims 1-4 or 6-7 or 9, wherein: the objective table assembly further comprises two fifth sliding seats for driving the objective table body to reciprocate along the direction approaching or separating from the liquid preparation control mechanism, a sliding rail is arranged below each fifth sliding seat, and each fifth sliding seat is connected to the corresponding sliding rail and reciprocates relative to the sliding rail; the fifth sliding seat is horizontally penetrated with a fifth screw rod, an internal thread is arranged in the fifth sliding seat, an external thread on the fifth screw rod is matched with an internal thread in the corresponding fifth sliding seat, and the fifth sliding seat is driven to reciprocate on the corresponding sliding rail by forward and backward rotation of the fifth screw rod.

11. The cartridge diagnostic test device of claim 10 wherein: a first elastic supporting component is arranged between the object stage body and the fifth sliding seats, the first elastic supporting component comprises a mounting plate arranged on each fifth sliding seat, at least one first support column is vertically arranged on the mounting plate, the object stage body is sleeved on the first support column and can lift along the first support column, a first spring is sleeved on the first support column, and the first spring is positioned between the object stage body and the corresponding mounting plate;

And/or a plurality of second elastic supporting components which enable the reaction control mechanism to elastically lift are arranged below the object stage body.