CN221056332U - Ultraviolet-visible absorption and fluorescence composite detection optical system - Google Patents
Ultraviolet-visible absorption and fluorescence composite detection optical system Download PDFInfo
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- CN221056332U CN221056332U CN202322598873.4U CN202322598873U CN221056332U CN 221056332 U CN221056332 U CN 221056332U CN 202322598873 U CN202322598873 U CN 202322598873U CN 221056332 U CN221056332 U CN 221056332U
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- fluorescence
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- 238000010521 absorption reaction Methods 0.000 title claims abstract description 51
- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 230000003287 optical effect Effects 0.000 title claims abstract description 23
- 239000002131 composite material Substances 0.000 title claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 15
- 238000009833 condensation Methods 0.000 claims abstract description 13
- 230000005494 condensation Effects 0.000 claims abstract description 13
- 239000013307 optical fiber Substances 0.000 claims description 17
- 230000008878 coupling Effects 0.000 claims description 12
- 238000010168 coupling process Methods 0.000 claims description 12
- 238000005859 coupling reaction Methods 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000005350 fused silica glass Substances 0.000 claims description 5
- 230000009977 dual effect Effects 0.000 claims description 4
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 230000005284 excitation Effects 0.000 description 10
- 238000001917 fluorescence detection Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000009537 plain noodles Nutrition 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 238000002189 fluorescence spectrum Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
Abstract
The utility model discloses an ultraviolet-visible absorption and fluorescence composite detection optical system. The ultraviolet-visible absorption and fluorescence composite detection optical system comprises an ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette, a signal light guide mechanism and a spectrometer, wherein the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette comprises a cuvette main body, the cuvette main body is provided with a light-passing plane, and three light-passing planes are provided with focusing elements; the signal light guide mechanism is at least used for collecting signal light emitted by the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette and guiding the signal light into the spectrometer. According to the utility model, the focusing elements are arranged on the three light-transmitting planes of the cuvette main body to form a whole, and the ultraviolet-visible absorption and fluorescence composite detection can be realized without adding additional converging elements and adjusting mechanisms or increasing the complexity of operation.
Description
Technical Field
The utility model belongs to the technical field of spectrum analysis, and particularly relates to an ultraviolet-visible absorption and fluorescence composite detection optical system.
Background
The common cuvette main part is cuboid structure, like patent CN 102866113A discloses 10MM standard cuvette structure, as shown in FIG. 1, including cuvette body 1, upper cover 2 lid dress is in the internal flange face of cuvette body 1, and the upper end of the logical plain noodles 3 of cuvette body 1 is provided with arrow structure 4, and the transparent surface 5 of logical plain noodles 3 of cuvette body 1 is the transparent structure of indent, and the non-logical plain noodles 6 equipartition of cuvette body 1 has vertical stripe recess 7, and the contact surface of upper cover 2 and cuvette body 1 is provided with at least two-layer concave-convex groove enclosed construction 8. In the optical detection processes of fluorescence, ultraviolet visible absorption and the like, the light beam horizontally passes through the cuvette, so that a better focusing effect cannot be achieved, and the fluorescence detection efficiency is low.
In the actual detection process, if the detection light needs to be focused in the cuvette, a focusing lens is additionally added, the complexity of an optical path is increased, as disclosed in patent CN 100588950C, a light-absorption and fluorescence spectrum composite detector is shown in fig. 2, and the detector comprises a radiation light source, the cuvette and a microprocessor, an absorption light sensor module and a fluorescence sensor module, wherein the positions of the absorption light sensor module and the fluorescence sensor module are ninety degrees each other and are connected with the microprocessor, the microprocessor is connected with the radiation light source through a pulse driving circuit, and focusing mirrors are respectively arranged between the cuvette surface and the radiation light source, the absorption light sensor module and the fluorescence sensor module.
Disclosure of Invention
The utility model mainly aims to provide an ultraviolet-visible absorption and fluorescence composite detection optical system so as to overcome the defects in the prior art.
In order to achieve the above purpose, the technical scheme adopted by the utility model comprises the following steps:
The utility model provides an ultraviolet-visible absorption and fluorescence composite detection optical system, which comprises an ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette, a signal light guide mechanism and a spectrometer, wherein the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette comprises a cuvette main body, the cuvette main body is provided with a light-passing plane, and focusing elements are arranged on three light-passing planes;
The signal light guide mechanism is at least used for collecting signal light emitted by the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette and guiding the signal light into the spectrometer.
Further, the focusing element is of a plano-convex structure.
Further, the plane of the focusing element is glued to the light passing plane of the cuvette body.
Further, the focusing element includes a hemispherical lens, a hemispherical cylindrical lens, a plano-convex lens, or a self-focusing lens.
Further, the focusing element is made of fused silica.
Further, the cuvette body comprises a light transmissive fused quartz or polymethyl methacrylate member.
Further, a polaroid or a filter is arranged between the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette and the signal light guide mechanism.
Further, the signal light guiding mechanism comprises an optical fiber coupling element and an optical fiber, and the optical fiber coupling element is arranged opposite to one focusing element in the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette.
Further, the fiber coupling element comprises a lens or a self-focusing lens.
Compared with the prior art, the utility model has the following beneficial effects:
(1) According to the utility model, the focusing elements are arranged on the three light-passing planes of the cuvette main body to form a whole, no additional converging elements or adjusting mechanisms are required to be added, the complexity of operation is not required to be improved, the ultraviolet-visible absorption and fluorescence composite detection can be realized, the detection light can be focused in the cuvette, the fluorescence excitation efficiency is improved, and a good detection effect is realized.
(2) The ultraviolet-visible absorption and fluorescence composite detection optical system simplifies the light path, makes the whole device more compact, reduces the use complexity, is suitable for the ultraviolet-visible absorption and fluorescence composite detection light path, can be used for detection of other optical properties, and can also be used for independent test of fluorescence or ultraviolet-visible absorption.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to the drawings without inventive effort to those skilled in the art.
FIG. 1 is a schematic diagram of a 10MM standard cuvette in the background of the application.
FIG. 2 is a schematic diagram of a composite detector of absorption light and fluorescence spectrum in the background of the application.
FIG. 3 is a schematic diagram of a dual-purpose UV-visible absorption and fluorescence detection optical system according to an embodiment of the present application.
Reference numerals illustrate: 1. the cuvette comprises a cuvette body, an upper cover, a light passing surface, an arrow structure, a light transmitting surface, a non-light passing surface, a stripe groove, a concave-convex groove sealing structure, a cuvette body, a focusing element, an optical fiber coupling element, an optical fiber, a spectrometer, a liquid to be measured, a fluorescent excitation light and an ultraviolet-visible absorption excitation light.
Detailed Description
The utility model will be more fully understood from the following detailed description, which should be read in conjunction with the accompanying drawings. Detailed embodiments of the present utility model are disclosed herein; however, it is to be understood that the disclosed embodiments are merely exemplary of the utility model, which may be embodied in various forms. Therefore, specific functional details disclosed herein are not to be interpreted as limiting, but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present utility model in virtually any appropriately detailed embodiment.
Examples
The embodiment provides a detection optical system for dual purposes of ultraviolet-visible absorption and fluorescence, which comprises a light-gathering cuvette for dual purposes of ultraviolet-visible absorption and fluorescence, a signal light guide mechanism and a spectrometer 13, wherein the signal light guide mechanism is at least used for collecting signal light emitted by the light-gathering cuvette for dual purposes of ultraviolet-visible absorption and fluorescence and guiding the signal light into the spectrometer; the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette comprises a cuvette main body 9, wherein the cross section of the cuvette main body 9 is square with a side length of 12mm, the wall thickness is 1mm, and the material is fused quartz; the cuvette main body 9 is provided with three light-transmitting planes, a focusing element 10 is respectively glued at the centers of the three light-transmitting planes, the focusing element 10 adopts a hemispherical lens with the radius of 3mm, and the hemispherical lens is made of molten stone.
The signal light guide mechanism comprises an optical fiber coupling element 11 and an optical fiber 12, wherein the optical fiber coupling element 11 is arranged opposite to one focusing element 10 in a light-gathering cuvette for ultraviolet-visible absorption and fluorescence, and the optical fiber coupling element 11 adopts a self-focusing lens.
The liquid 14 to be measured is contained in the cuvette body 9, and the detection light passes through the focusing element 10 and is focused in the cuvette. For fluorescence detection, focusing can effectively improve the excitation efficiency of fluorescence, and high-efficiency fluorescence detection is realized. For optical characteristic detection such as ultraviolet visible absorption, the focusing element can also concentrate light beams, so that a better detection effect is achieved.
Specifically, for fluorescence detection, after fluorescence excitation light 15 is incident, the fluorescence excitation light is focused in the cuvette through a focusing element 10 on the surface of the light-gathering cuvette, and an excited fluorescence signal is emitted along the direction perpendicular to the fluorescence excitation light 15, is collimated through the focusing element on the surface of the cuvette, enters an optical fiber 12 through an optical fiber coupling element 11, and is detected by a spectrometer 13.
For ultraviolet-visible absorption, after the ultraviolet-visible absorption excitation light 16 is incident, the ultraviolet-visible absorption excitation light is focused in the cuvette through the focusing element 10 on the surface of the condensing cuvette, the signal light is emitted in the same direction as the incident direction of the ultraviolet-visible absorption excitation light 16, is collimated through the focusing element 10 on the surface of the cuvette, enters the optical fiber 12 through the optical fiber coupling element 11, and is detected by the spectrometer 13.
While the utility model has been described with reference to an illustrative embodiment, it will be understood by those skilled in the art that various other changes, omissions and/or additions may be made and substantial equivalents may be substituted for elements thereof without departing from the spirit and scope of the utility model. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the utility model without departing from the scope thereof. Therefore, it is intended that the utility model not be limited to the particular embodiment disclosed for carrying out this utility model, but that the utility model will include all embodiments falling within the scope of the appended claims. Moreover, unless specifically stated any use of the terms first, second, etc. do not denote any order or importance, but rather the terms first, second, etc. are used to distinguish one element from another.
Claims (9)
1. The ultraviolet-visible absorption and fluorescence composite detection optical system is characterized by comprising an ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette, a signal light guide mechanism and a spectrometer (13), wherein the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette comprises a cuvette main body (9), the cuvette main body (9) is provided with a light-transmitting plane, and focusing elements (10) are arranged on three light-transmitting planes;
The signal light guiding mechanism is at least used for collecting signal light emitted by the light-gathering cuvette with dual purposes of ultraviolet-visible absorption and fluorescence and guiding the signal light into the spectrometer (13).
2. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 1, wherein: the focusing element (10) is of plano-convex configuration.
3. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 2, wherein: the plane of the focusing element (10) is glued to the light-transmitting plane of the cuvette body (9).
4. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 2, wherein: the focusing element (10) comprises a hemispherical lens, a hemispherical cylindrical lens, a plano-convex lens or a self-focusing lens.
5. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 2, wherein: the focusing element (10) is made of fused silica.
6. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 1, wherein: the cuvette body (9) comprises a light-transmissive fused quartz or polymethyl methacrylate member.
7. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 1, wherein: a polaroid or a filter is also arranged between the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette and the signal light guide mechanism.
8. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 7, wherein: the signal light guide mechanism comprises an optical fiber coupling element (11) and an optical fiber (12), and the optical fiber coupling element (11) is arranged opposite to one focusing element (10) in the ultraviolet-visible absorption and fluorescence dual-purpose condensation cuvette.
9. The ultraviolet-visible absorption and fluorescence composite detection optical system according to claim 8, wherein: the optical fiber coupling element (11) comprises a lens or a self-focusing lens.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221056332U true CN221056332U (en) | 2024-05-31 |
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