CN220999685U - Multilayer cell culture bottle with under-lens observation port - Google Patents
Multilayer cell culture bottle with under-lens observation port Download PDFInfo
- Publication number
- CN220999685U CN220999685U CN202322815539.XU CN202322815539U CN220999685U CN 220999685 U CN220999685 U CN 220999685U CN 202322815539 U CN202322815539 U CN 202322815539U CN 220999685 U CN220999685 U CN 220999685U
- Authority
- CN
- China
- Prior art keywords
- bottle
- baffle
- cell culture
- culture
- under
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 21
- 238000005192 partition Methods 0.000 claims abstract description 46
- 239000010410 layer Substances 0.000 claims description 30
- 239000002356 single layer Substances 0.000 claims description 5
- 238000010030 laminating Methods 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims 6
- 210000004027 cell Anatomy 0.000 description 12
- 239000007788 liquid Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell culture, and discloses a multilayer cell culture bottle with an under-lens observation port, which comprises a bottle body, wherein a bottle opening is formed in the bottle body, a bottle cap is arranged on the bottle opening, a plurality of partition boards are arranged in the bottle body at intervals to form a plurality of culture layers together with the bottle bottom and the bottle top of the bottle body, one observation port is formed in each culture layer, one end of each observation port, which is away from the corresponding culture layer, is communicated with the bottle bottom or the bottle top on the corresponding side, and the device solves the problem that the multilayer cell culture bottle can only observe the cell culture condition through naked eyes.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a multilayer cell culture bottle with a microscopic observation port.
Background
In the process of cell culture, cells are required to be placed in a culture solution to simulate in-vivo environment, so that the cells grow and reproduce in the external environment, and some mammalian cells are required to be subjected to surface adherence culture, so that a culture bottle for providing an in-vitro cell culture environment is required, normal survival and reproduction of the cells are ensured, and when a large amount of cell culture is performed, a plurality of layers of cell culture bottles are generally used to save laboratory space.
At present, the Chinese patent application No. 20232006679. X discloses a multi-layer cell factory culture bottle, a plurality of culture bottles are fixed through clamping, each culture bottle is communicated through a lower seepage hole at the bottom, so that the distribution of culture solution is realized, any number of culture bottles can be selected for assembly according to requirements, the space utilization rate is improved, but the multi-layer structure enables the culture bottles not to be observed under a microscope, the accurate growth state of the culture bottles cannot be confirmed only through naked eyes, and the culture failure is easy to cause.
Disclosure of utility model
Aiming at the defects existing in the prior art, the utility model aims to provide a multilayer cell culture bottle with a mirror observation port, which saves space and is convenient for accurate observation.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
The utility model provides a multilayer cell culture bottle with view port under mirror, includes the bottle, be provided with the bottleneck on the bottle, install the bottle lid on the bottleneck, the internal interval of bottle is provided with a plurality of baffles, a plurality of the baffle forms a plurality of culture layers jointly with the bottle end and the bottle top of bottle, every all be provided with an observation port on the culture layer, the one end that the observation port deviates from corresponding culture layer is linked together with the bottle end or the bottle top of corresponding side.
The utility model is further provided with: the viewing aperture is provided with and cuts off the post down, it all perpendicular to baffle setting to go up cuts off the post and cuts off the post down, cultivate layer deviates from bottle end one side and is face, opposite side for down the face, it cuts off post intercommunication bottle top and last face to go up, cut off post intercommunication bottle end and face down, cultivate layer department at the viewing aperture and form single-layer structure.
The utility model is further provided with: a gap is arranged between one end of the partition board facing the bottle mouth and the bottle body, and a second convex edge is connected between the partition board and the bottle body, the edge of the second convex edge is arranged along the partition board, the two ends of the second convex edge are respectively connected with the inner wall of the bottle body, other sides of the partition board are all attached to the inner wall of the bottle body, through holes are formed in one corner, deviating from the bottle mouth, of the partition board, a first protruding edge is arranged along the periphery of the through holes, and the first protruding edge and the second protruding edge extend to one side of the top of the bottle.
The utility model is further provided with: the bottle cap is provided with a plurality of ventilation holes, and filter paper is arranged between the bottle cap and the bottle mouth.
The utility model is further provided with: the bottle is characterized in that the bottle opening is formed in one side, close to the bottle top, of the bottle body, a guide plate is arranged in the bottle body, one end, facing the bottle opening, of the guide plate is attached to the bottle top, and the other end of the guide plate faces the partition plate to be bent.
The utility model has the advantages that:
1. a plurality of partition boards are arranged in the bottle body, the partition boards divide the bottle body into a plurality of culture layers, and through holes are formed in one corner of the bottom of each partition board to realize the mixing and distribution of cell liquid among the culture layers, so that the space utilization rate is improved, and the quantity of cells which can be cultured in one culture bottle is increased;
2. All be provided with the viewing aperture on every culture layer, the viewing aperture includes cuts off post and cuts off the post down, cuts off the post on the one end of last and connects the face of going up of culture layer, the bottle top is connected to the other end, cuts off the post down the one end of post and connects the face of going down of culture layer, the bottle end is connected to the other end, cuts off the post and cuts off the post down through last for the upper and lower face of every culture layer forms the monolayer structure in viewing aperture department, is convenient for observe the cultivation condition of cell under the microscope.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present utility model;
FIG. 2 is a cross-sectional view taken along line A-A of FIG. 1;
FIG. 3 is a cross-sectional view taken along line B-B of FIG. 1;
FIG. 4 is a cross-sectional view taken along line C-C of FIG. 1;
In the figure: 1. a bottle body; 11. a bottle mouth; 12. a bottle bottom; 13. a bottle top; 2. a bottle cap; 3. a filter paper; 4. a deflector; 5. a partition plate; 51. a first convex edge; 52. a through-flow hole; 53. a second convex edge; 6. an observation port; 61. an upper partition column; 62. and a lower partition column.
Detailed Description
It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other. The utility model will be described in detail below with reference to the drawings in connection with embodiments.
It is noted that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless otherwise indicated.
In the present utility model, unless otherwise indicated, the terms "upper" and "lower" are used generally with respect to the directions shown in the drawings, or with respect to the vertical, vertical or gravitational directions; also, for ease of understanding and description, "left, right" is generally directed to the left, right as shown in the drawings; "inner and outer" refer to inner and outer relative to the outline of the components themselves, but the above-described orientation terms are not intended to limit the present utility model.
Referring to fig. 1-4, the present utility model provides the following technical solutions:
The utility model provides a multilayer cell culture bottle with observation mouth under mirror, includes bottle 1, and the upper bottle surface of bottle 1 is bottle top 13, and lower bottle surface is bottle bottom 12, and bottleneck 11 has been seted up to one side that bottle 1 side is close to bottle top 13, installs bottle lid 2 on the bottleneck 11, is provided with a plurality of bleeder vents on the bottle lid 2, is provided with filter paper 3 between bottle lid 2 and the bottleneck 11, and the bleeder vent cooperation filter paper 3 on the bottle lid 2 provides clean air for the cells of the interior culture of bottle 1, guarantees its normal development.
A plurality of partition boards 5 are arranged in the bottle body 1 at intervals, the partition boards 5 are parallel to the bottle bottom 12, the partition boards 5 and the adjacent partition boards 5 or the bottle bottom 12 or the bottle top 13 form a plurality of culture layers together, each culture layer is provided with an observation port 6, one end of each observation port 6, which is away from the corresponding culture layer, is communicated with the bottle bottom 12 or the bottle top 13 on the corresponding side, so that the culture layer forms a single-layer structure at the corresponding observation port 6, and is convenient to observe under a microscope;
The viewing port 6 comprises an upper partition column 61 and a lower partition column 62, the upper partition column 61 and the lower partition column 62 are perpendicular to the partition plate 5, the plate surface on the upper side of the culture layer is an upper plate surface, the plate surface on the lower side of the culture layer is a lower plate surface, the upper partition column 61 is communicated with the upper plate surface and the bottle top 13, the lower partition column is communicated with the lower plate surface and the bottle bottom 12, the upper partition column 61 and the lower partition column 62 are connected with the lower partition column and penetrate through the partition plate 5 to form an integral structure, no gap is reserved at the viewing port 6 of the bottle body 1, external pollution is avoided, and the culture quality is guaranteed.
The baffle 5 is provided with the space towards between the one end of bottleneck 11 and the bottle 1 to be connected with protruding edge two 53, protruding edge two 53 sets up along the edge of baffle 5, both ends are connected with the inner wall of bottle 1 respectively, other sides of baffle 5 all set up with the inner wall laminating of bottle 1, through-hole 52 has been seted up on baffle 5 deviates from one of them angle of bottleneck 11, make the cell sap in the bottle 1 can realize mixing and distribute each culture layer through-hole 52, the hole edge of through-hole 52 is equipped with protruding edge one 51, protruding edge one 51 and protruding edge two 53 all extend to bottle top 13 one side, make baffle 5 cooperate the inner wall of bottle 1 and the protruding edge one 51 and the protruding edge two 53 of both ends form the petri dish column structure, can keep a certain amount of cell culture fluid, thereby carry out cell culture.
The bottleneck 11 is offered in the bottle 1 and is close to the one side of bottle top 13, is provided with guide plate 4 in the bottle 1, and guide plate 4 is laminated mutually with bottle top 13 towards the one end of bottleneck 11, and the other end is crooked towards baffle 5, the filling of cell culture liquid of being convenient for.
Specifically, a plurality of partition boards 5 are arranged in the bottle body 1, the partition boards 5 divide the interior of the bottle body 1 into a plurality of culture layers, and through holes 52 are formed in one corner of the bottom of the partition boards 5 to realize the mixing and distribution of cell liquid among the culture layers, so that the space utilization rate is improved, and the number of cells which can be cultured in one culture bottle is increased;
The observation port 6 is formed in each culture layer, the observation port 6 comprises an upper partition column 61 and a lower partition column 62, one end of the upper partition column 61 is connected with an upper plate surface of the culture layer, the other end of the upper partition column is connected with a bottle top 13, one end of the lower partition column 62 is connected with a lower plate surface of the culture layer, the other end of the lower partition column is connected with a bottle bottom 12, the upper partition column 61 and the lower partition column 62 are used for enabling the upper plate surface and the lower plate surface of each culture layer to form a single-layer structure at the observation port 6, and the culture condition of cells can be observed under a microscope conveniently.
It will be apparent that the embodiments described above are merely some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present utility model without making any inventive effort, shall fall within the scope of the present utility model.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the application described herein may be implemented in sequences other than those illustrated or otherwise described herein.
The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (5)
1. Multilayer cell culture bottle with view port under mirror, including bottle (1), its characterized in that: be provided with bottleneck (11) on bottle (1), install bottle lid (2) on bottleneck (11), the interval is provided with a plurality of baffles (5) in bottle (1), a plurality of baffle (5) form a plurality of culture medium layers jointly with bottle end (12) and bottle top (13) of bottle (1), every all be provided with one on the culture medium layer and observe mouth (6), the one end that the culture medium layer was kept away from to observe mouth (6) is linked together with bottle end (12) or bottle top (13) of corresponding side.
2. The multi-layered cell culture flask with an under-the-lens viewing port of claim 1, wherein: the utility model discloses a culture medium, including bottle bottom (12), viewing aperture (6), upper partition post (61) and lower partition post (62) are provided with, upper partition post (61) and lower partition post (62) all perpendicular to baffle (5) set up, culture medium deviates from bottle bottom (12) one side and is the face, the opposite side is the face down, upper partition post (61) intercommunication bottle top (13) and face down, lower partition post (62) intercommunication bottle bottom (12) and face down, the culture medium forms single-layer structure in viewing aperture (6) department.
3. A multi-layered cell culture flask with an under-the-lens viewing port according to claim 2, wherein: the utility model discloses a bottle, including bottle top (13) and bottle, including baffle (5), bottle top (11) and baffle (5), baffle (5) are provided with the space between one end towards bottleneck (11) and bottle (1) to be connected with protruding two (53), protruding two (53) are connected with the inner wall of bottle (1) respectively along the edge setting of baffle (5), other sides of baffle (5) all set up with the inner wall laminating of bottle (1), through-hole (52) have been seted up on one of them angle that baffle (5) deviate from bottleneck (11), the hole edge of through-hole (52) is equipped with protruding edge one (51), protruding edge one (51) and protruding two (53) all extend to bottle top (13) one side.
4. The multi-layered cell culture flask with an under-the-lens viewing port of claim 1, wherein: a plurality of ventilation holes are formed in the bottle cap (2), and filter paper (3) is arranged between the bottle cap (2) and the bottle mouth (11).
5. The multi-layered cell culture flask with an under-the-lens viewing port of claim 1, wherein: the bottle is characterized in that the bottle opening (11) is formed in one side, close to the bottle top (13), of the bottle body (1), a guide plate (4) is arranged in the bottle body (1), one end, facing the bottle opening (11), of the guide plate (4) is attached to the bottle top (13), and the other end of the guide plate is bent towards the partition plate (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322815539.XU CN220999685U (en) | 2023-10-20 | 2023-10-20 | Multilayer cell culture bottle with under-lens observation port |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322815539.XU CN220999685U (en) | 2023-10-20 | 2023-10-20 | Multilayer cell culture bottle with under-lens observation port |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220999685U true CN220999685U (en) | 2024-05-24 |
Family
ID=91126968
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322815539.XU Active CN220999685U (en) | 2023-10-20 | 2023-10-20 | Multilayer cell culture bottle with under-lens observation port |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220999685U (en) |
-
2023
- 2023-10-20 CN CN202322815539.XU patent/CN220999685U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11905506B2 (en) | Multilayered cell culture apparatus | |
| US7897379B2 (en) | Device and method for reducing bubble formation in cell culture | |
| DE69615655T2 (en) | BIOLOGICAL ANALYTICAL DEVICE WITH IMPROVED PREVENTION OF CONTAMINATION | |
| US20100055790A1 (en) | Cell culture apparatus and associated methods | |
| CN101611132A (en) | The method of highly efficient gas permeable devices and culturing cell | |
| US20240117288A1 (en) | Culture devices | |
| CN220999685U (en) | Multilayer cell culture bottle with under-lens observation port | |
| WO2018057769A1 (en) | Gravity flow cell culture devices, systems and methods of use thereof | |
| CN205035357U (en) | Deposit device of culture dish | |
| US20240091778A1 (en) | Cell culture vessel | |
| CN107966581B (en) | Zebra fish embryo microplate arrangement adding device | |
| CN217535988U (en) | Co-culture dish for multiple cell types | |
| CN205035359U (en) | A device for placing culture dish | |
| JP2000093159A (en) | Device for incubating and condensing nonadherent cell | |
| CN106754358B (en) | Cell co-culture bottle convenient for transferring cells | |
| CN220413411U (en) | Embryo culture dish | |
| CN221822219U (en) | Novel cell factory | |
| CN220335212U (en) | Organ-like culture device | |
| CN216972561U (en) | Culture device | |
| CN205035358U (en) | Culture dish rack | |
| CN212610699U (en) | 3 layers of cell culture bottles in fillet | |
| CN219653038U (en) | Detachable in-vitro micro-tissue dynamic co-culture device | |
| CN221822217U (en) | Stem cell culture device | |
| CN216005848U (en) | Cell biological assay culture dish | |
| CN216712123U (en) | Ventilative digestion culture dish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |