CN220977011U - Combined pore plate for tubule formation experiment - Google Patents
Combined pore plate for tubule formation experiment Download PDFInfo
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- CN220977011U CN220977011U CN202322586850.1U CN202322586850U CN220977011U CN 220977011 U CN220977011 U CN 220977011U CN 202322586850 U CN202322586850 U CN 202322586850U CN 220977011 U CN220977011 U CN 220977011U
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- tubule formation
- layer plate
- holes
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- 238000002474 experimental method Methods 0.000 title claims abstract description 40
- 239000011148 porous material Substances 0.000 title claims abstract description 24
- 210000005239 tubule Anatomy 0.000 title claims abstract description 24
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 20
- 108010082117 matrigel Proteins 0.000 claims abstract description 18
- 239000002131 composite material Substances 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 5
- 239000006285 cell suspension Substances 0.000 abstract description 6
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000033115 angiogenesis Effects 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 239000003292 glue Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 210000002469 basement membrane Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 210000003771 C cell Anatomy 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
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- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 230000021368 organ growth Effects 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a combined pore plate for a tubule formation experiment, and belongs to the technical field of biological experimental instruments. The combined plate comprises an upper plate and a lower plate, wherein the upper plate comprises a plate body, a plurality of step holes for containing matrigel are formed in the plate body, the step holes are formed by communicating concentric first through holes and second through holes, and the diameter of the first through holes is larger than that of the second through holes; the lower layer plate comprises a bottom plate and brackets on two sides, and a concave area for accommodating the upper layer plate is formed between the bottom plate and the brackets on two sides. When a tubule formation experiment is carried out, the upper layer plate and the lower layer plate are tightly attached, matrigel and cell suspension are sequentially added into the step hole, and after standing for 4 hours, the result is observed under a microscope. The upper layer plate and the lower layer plate are combined for experiment, and the upper layer plate and the lower layer plate are separated for cleaning, so that the device is convenient to use, can be repeatedly utilized, and saves the experiment cost.
Description
Technical Field
The utility model belongs to the technical field of biological experimental instruments, and particularly relates to a combined pore plate for a tubule formation experiment.
Background
Angiogenesis (Angiogenesis) is the development of new blood vessels from existing capillaries or post-capillary veins and is important for a variety of processes including organ growth, embryonic development and wound healing. Matrigel is a basement membrane component extracted from mouse tumor tissues, is expensive, is solid at-20 ℃ and liquid at 4 ℃, and is polymerized to form a three-dimensional matrix (solid) with biological activity at room temperature (37 ℃), so that the matrigel simulates the structure, composition, physical characteristics and functions of in-vivo cell basement membranes, is favorable for the culture and differentiation of in-vitro cells, and can be used for researching cell morphology, biochemical functions, migration, infection, gene expression and the like.
The tubule formation experiment (Tube formation assay) is a classical molecular biology experiment for simulating angiogenesis in vitro, and reflects the ability of angiogenesis in vitro by seeding endothelial cells into 96-well plates with matrigel laid down to form a three-dimensional network structure over a period of time. The steps of the tubule formation experiment are typically: freezing and thawing matrigel overnight at 4 ℃ in a refrigerator on the first day of the experiment, adding the matrigel into a 96-well plate at a low temperature of 50-100 mu L/hole, then placing the 96-well plate in a 37 ℃ cell culture box for culturing until solidification, digesting cells and counting, taking out the 96-well plate after the cells are solidified by gelatinization, adding 50-100 mu L of cell suspension into each hole, placing the cell culture box for culturing after marking, and taking a proper time point for photographing after a small tube is generated.
Because the existing 96-well plates (see fig. 1) are all integrally formed, the existing 96-well plates are difficult to clean and cannot be reused when performing a tubule formation experiment, so that the experiment cost is increased.
Disclosure of utility model
In order to overcome the defects of the prior art, the utility model aims to provide a combined pore plate for a small tube forming experiment, which solves the problems that the existing pore plate for the small tube forming experiment is difficult to clean and cannot be recycled.
In order to achieve the above purpose, the utility model is realized by adopting the following technical scheme:
The utility model discloses a combined pore plate for a tubule forming experiment, which comprises an upper layer plate and a lower layer plate, wherein the upper layer plate comprises a plate body, and a plurality of sample adding holes are formed in the plate body; the lower layer plate comprises a bottom plate and brackets on two sides of the bottom plate, and a concave area for accommodating the upper layer plate is formed between the bottom plate and the brackets on two sides.
Preferably, the sample adding hole is a step hole, and the step hole is formed by communicating a first through hole and a second through hole which are concentric.
Preferably, the diameter of the first through hole is larger than the diameter of the second through hole.
Further preferably, the first through hole has a diameter of 7mm and a height of 8mm, and the second through hole has a diameter of 5mm and a height of 1mm.
Preferably, the bottom of the sample adding hole is attached to the upper surface of the bottom plate to jointly form a space for accommodating matrigel.
Preferably, the length of the plate body is 35-55 mm, the width is 22-25 mm, and the height is 9mm.
Preferably, the 8 sample application holes are uniformly distributed on the plate body.
Preferably, the length of the bottom plate is consistent with the length of the plate body, the width is 24-31 mm, and the height is 1-3 mm.
Preferably, the length and the height of the bracket are consistent with those of the plate body, and the width is 1-3 mm.
Preferably, the composite well plate is made of glass.
Compared with the prior art, the utility model has the following beneficial effects:
The combined pore plate for the tubule forming experiment provided by the utility model adopts a mode of combining the upper layer plate and the lower layer plate to perform the experiment, the upper layer plate and the lower layer plate are separated to be cleaned after the experiment is finished, and residual matrigel and cell suspension can be removed through a cotton swab and alcohol, so that the combined pore plate is convenient to use, can be repeatedly used, and saves the experiment cost.
Further, the step holes are formed through the first through holes and the second through holes, so that the consumption of matrigel can be reduced, and the experiment cost is further saved. The design of step hole laminating bottom plate for through instilling into a small amount of liquid at the contact surface during the experiment, just can be in the same place with the laminating of lower floor board with the help of liquid surface tension, avoid the influence that the unsmooth liquid level that causes because of liquid surface tension when the microscope observes, make things convenient for experimenter to record the result, improve experimental efficiency. Compared with the existing orifice plate for experiment formed by small tubes, the combined orifice plate consumes less matrigel, can greatly save the dosage of matrigel in the experiment, is less prone to generating concave liquid level, has higher experimental result and efficiency, and can take a picture with more fields of vision.
Furthermore, the combined pore plate is made of super-transparent glass materials, so that photographing results are not affected.
Drawings
FIG. 1 is a schematic diagram of a conventional 96-well plate;
FIG. 2 is a schematic view of the upper and lower plates of the present utility model assembled into a composite orifice plate;
FIG. 3 is a schematic view of the structure of the upper plate in the combined orifice plate of the present utility model;
FIG. 4 is a top view of the upper plate in the composite orifice plate of the present utility model;
FIG. 5 is a schematic view showing the structure of the stepped hole in the upper plate of the present utility model;
FIG. 6 is a schematic view of the structure of the lower plate in the combined orifice plate of the present utility model;
FIG. 7 is a side view of a lower plate in the composite orifice plate of the present utility model;
FIG. 8 is a side view of a lower plate in the composite orifice plate of the present utility model;
FIG. 9 is a schematic diagram showing the structure of the sample-adding stepped hole and the bottom plate of the present utility model.
Wherein: 1-upper plate; 2-a lower plate; 3-step holes; 4-a bracket; 5-a plate body; 6-a bottom plate; 7-a first through hole; 8-a second through hole; 9-matrigel; 10-cell suspension.
Detailed Description
In order that those skilled in the art will better understand the present utility model, a technical solution in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present utility model without making any inventive effort, shall fall within the scope of the present utility model.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present utility model and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the utility model described herein may be implemented in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The utility model provides a combined pore plate for a tubule forming experiment, which is shown in fig. 2 and is made of high-light-transmittance ultra-white glass, and the combined pore plate comprises an upper layer plate 1 and a lower layer plate 2 which are used in combination. Referring to fig. 3 and 4, the upper plate 1 includes a plate body 5, the plate body 5 has a rectangular structure with a length of 35-55 mm, a width of 22-25 mm and a height of 9mm, a plurality of step holes 3 for containing matrigel are uniformly formed in the plate body 5, the number of the step holes 3 includes but is not limited to 8, the center distances of the step holes 3 are equal, and the bottoms of the step holes 3 are attached to the upper surface of the bottom plate 6 to jointly form a space for containing matrigel. Referring to fig. 5, the stepped hole 3 is formed by communicating concentric first and second through holes 7 and 8, and the diameter of the first through hole 7 is larger than that of the second through hole 8. Referring to fig. 6 to 8, the side surface of the lower layer plate 2 is in a concave structure, the lower layer plate 2 comprises a bottom plate 6 and brackets 4 on two sides of the bottom plate 6, the bottom plate 6 is bonded with the brackets 4, a concave area formed by the bottom plate 6 and the brackets 4 on two sides is used for accommodating the upper layer plate 1, the length of the bottom plate 6 is consistent with the length of the plate body 5, the width is 24-31 mm, and the height is 1-3 mm; the length and the height of the bracket 4 are consistent with those of the plate body 5, and the width is 1-3 mm. The upper layer plate 1 and the lower layer plate 2 of the combined pore plate are required to be combined when in use, and can be used after being attached, and recovered after the experiment is finished.
Referring to fig. 9, an upper layer plate 1 and a lower layer plate 2 are cleaned, the upper layer plate 1 and the lower layer plate 2 are tightly attached to each other, the lengths and the widths of the upper layer plate 1 and the lower layer plate 2 are in one-to-one correspondence, the bottoms of step holes 3 of the upper layer plate 1 are completely attached to the upper surface of a bottom plate 6, then matrix glue is dripped into each step hole 3 according to the experimental requirement, so that the matrix glue fills a second through hole 8 of the step hole 3, the second through hole is placed in the environment of 37 ℃, after the matrix glue is solidified, cell suspension is added into a first through hole 7, and the occurrence of tube forming is waited.
Example 1
The utility model provides a combined pore plate for a tubule forming experiment, which is shown in fig. 2 and is made of high-light-transmittance ultra-white glass, and the combined pore plate comprises an upper layer plate 1 and a lower layer plate 2 which are used in combination. The upper plate 1 comprises a plate body 5, the plate body 5 is of a cuboid structure with the length of 45mm, the width of 25mm and the height of 9mm, 8 stepped holes 3 for containing matrigel are uniformly formed in the plate body 5, and the bottoms of the stepped holes 3 are attached to the upper surface of a bottom plate 6 to jointly form a space for containing matrigel. The step hole 3 is formed by communicating a first through hole 7 and a second through hole 8 which are concentric, wherein the diameter of the first through hole 7 is 7mm, the height is 8mm, the diameter of the second through hole 8 is 5mm, and the height is 1mm. The side surface of the lower layer plate 2 is of a concave structure, the lower layer plate 2 comprises a bottom plate 6 and brackets 4 on two sides, the bottom plate 6 is bonded with the brackets 4, a concave area formed by the bottom plate 6 and the brackets 4 on two sides is used for accommodating the upper layer plate 1, the length of the bottom plate 6 is 45mm, the width is 29mm, and the height is 2mm; the length of the bracket 4 is 45mm, the width is 2mm, and the height is 9mm. The upper layer plate 1 and the lower layer plate 2 of the combined pore plate are required to be combined when in use, and the upper layer plate and the lower layer plate can be used after being attached.
And after the experiment is finished, recovering the combined pore plate, separating the upper layer plate 1 from the lower layer plate 2, removing residual matrigel and cell suspension through a cotton swab and alcohol, airing, and covering the surface of the combined pore plate by using a shell of the combined pore plate for the next use.
The combined pore plate for the tubule formation experiment is adopted to carry out the tubule formation experiment, meanwhile, the existing 96 pore plate is used as a comparison, and experimental results show that in the existing 96 pore plate photographing result, although the center of the hole is well focused, the liquid level at the edge of the hole is inclined, so that poor focusing is caused. In the photographing result of the combined pore plate, the center and the edge of the hole are well focused, which indicates that the arrangement of the step hole is not easy to generate concave liquid level in use.
The above is only for illustrating the technical idea of the present utility model, and the protection scope of the present utility model is not limited by this, and any modification made on the basis of the technical scheme according to the technical idea of the present utility model falls within the protection scope of the claims of the present utility model.
Claims (10)
1. The combined pore plate for the tubule forming experiment is characterized by comprising an upper layer plate (1) and a lower layer plate (2), wherein the upper layer plate (1) comprises a plate body (5), and a plurality of sample adding holes are formed in the plate body (5); the lower layer plate (2) comprises a bottom plate (6) and brackets (4) on two sides of the bottom plate (6), and a concave area for accommodating the upper layer plate (1) is formed between the bottom plate (6) and the brackets (4) on two sides.
2. A combined well plate for tubule formation experiments as claimed in claim 1, characterized in that the sample addition well is a stepped well (3), the stepped well (3) being formed by communicating concentric first and second through holes (7, 8).
3. A combined orifice plate for tubule formation experiments as claimed in claim 2, characterized in that the diameter of the first through hole (7) is larger than the diameter of the second through hole (8).
4. A combined orifice plate for tubule formation experiments as claimed in claim 3, characterized in that the first through-hole (7) has a diameter of 7mm and a height of 8mm, and the second through-hole (8) has a diameter of 5mm and a height of 1mm.
5. A combined well plate for tubule formation experiments as claimed in any one of claims 1 to 4, wherein the bottom of the well is attached to the upper surface of the base plate (6) to together form a space for receiving matrigel.
6. A combined orifice plate for tubule formation experiments as claimed in any one of claims 1 to 4, wherein the plate body (5) is 35 to 55mm long, 22 to 25mm wide and 9mm high.
7. A composite well plate for tubule formation experiments as claimed in any one of claims 1 to 4, wherein 8 wells are evenly distributed on the plate body (5).
8. A combined orifice plate for tubule formation experiments as claimed in any one of claims 1 to 4, wherein the base plate (6) is of length consistent with the length of the plate body (5), 24 to 31mm wide and 1 to 3mm high.
9. A combined orifice plate for tubule formation experiments as claimed in any one of claims 1 to 4, wherein the length and height of the support (4) is in accordance with the length and height of the plate body (5) and the width is 1 to 3mm.
10. A composite well plate for use in tubule formation experiments as claimed in any one of claims 1 to 4, wherein the composite well plate is made of glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322586850.1U CN220977011U (en) | 2023-09-22 | 2023-09-22 | Combined pore plate for tubule formation experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322586850.1U CN220977011U (en) | 2023-09-22 | 2023-09-22 | Combined pore plate for tubule formation experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220977011U true CN220977011U (en) | 2024-05-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322586850.1U Active CN220977011U (en) | 2023-09-22 | 2023-09-22 | Combined pore plate for tubule formation experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220977011U (en) |
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2023
- 2023-09-22 CN CN202322586850.1U patent/CN220977011U/en active Active
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