CN220819610U

CN220819610U - Measurement system and sample analyzer

Info

Publication number: CN220819610U
Application number: CN202320177955.9U
Authority: CN
Inventors: 董钊夺; 钟勇锋; 甘泉
Original assignee: Edan Instruments Inc
Current assignee: Edan Instruments Inc
Priority date: 2023-01-18
Filing date: 2023-01-18
Publication date: 2024-04-19
Anticipated expiration: 2033-01-18

Abstract

The application provides a measurement system and a sample analyzer. The measurement system includes: a container; sample supply means configured to add a sample to be measured to the container; a reagent supply configured to supply a reagent and add the reagent to the container; and a mixing device connected to the bottom of the container and comprising: the mixing component is connected with the bottom of the container; and a mixing power member connected with the mixing assembly, the mixing assembly being configured to store the gas entering through the container, and the mixing power member being configured to inject the gas into the container through the mixing assembly through the bottom of the container to mix the reagent and the sample to be measured. The gas is injected into the container through the bottom of the container by controlling the mixing power piece through the mixing assembly, so that the gas rises from bottom to top, bubbles rise under the action of buoyancy, turbulence is formed locally, a sample to be tested is fully mixed with the reagent, the measurement accuracy is improved, the risk of pipeline blockage or carrying pollution caused by the mixing process is reduced, and the cleaning difficulty is reduced.

Description

Measurement system and sample analyzer

Technical Field

The application relates to the technical field of medical detection, in particular to a measurement system and a sample analyzer.

Background

Related sample analyzers typically employ transmission or scattering immunoturbidimetry to detect and analyze a specific protein in a blood sample. In addition, a latex reagent (a suspension containing latex particles, also called a latex antibody reagent) is used for the detection process to participate in the reaction. The latex reagent has fluid properties of high viscosity and high density, so that the latex reagent is not favorable for fully and uniformly mixing the blood sample with the latex reagent. However, if the blood sample and the latex reagent are not sufficiently mixed, the reaction between them is not complete, resulting in inaccurate detection results.

In some of the related sample analyzers, the sample is diluted and incubated by mixing using a stirring rod, a stirrer or a suction and discharge mixing method. In the structure of the sample analyzer, more than two kinds of liquid are added through a sampling needle, then the sampling needle sucks air and inserts the needle end into the liquid, so that bubbles are beaten out by swinging the sampling needle to mix the liquid uniformly.

However, when the stirring rod or the stirring rod is used for stirring the mixed reagent, a motor and a rotating mechanism assembly are needed, and the motor and the rotating mechanism are large in size, so that the requirement on the volume space of the instrument is high, and the stirring rod is difficult to clean due to the fact that the latex reagent is large in viscosity, and an additional cleaning pool or a cleaning device is needed for cleaning the stirring rod. The stirrer is easy to scratch the light detection area in the stirring process, and the detection result is influenced.

In addition, when the sucking and spitting uniform mixing reagent is used, the diluent pipeline in the sample analyzer is filled with diluent, so that on one hand, the sample is in direct contact with the diluent, the sample liquid is easily diluted, and the sample detection accuracy is influenced; on the other hand, the insoluble compound generated by the reaction of the sample and the latex reagent is easy to adhere to the inner wall of the sucking and spitting uniform mixing pipeline, which is not beneficial to the cleaning of the pipeline and has the risk of carrying pollution.

Disclosure of utility model

The application mainly solves the technical problem of providing a measuring system, which fully mixes a sample to be measured and a reagent by injecting gas into a mixing mode of a container, so that the sample to be measured and the reagent react more fully, thereby effectively improving the measuring accuracy, reducing the risk of pipeline blockage or pollution carrying caused by the mixing process, and reducing the cleaning difficulty.

In order to solve the technical problems, an aspect of an embodiment of the present application provides a measurement system. The measurement system includes: a container; sample supply means configured to add a sample to be measured to the container; a reagent supply configured to supply a reagent and add the reagent to the container; and a mixing device connected to the bottom of the container, and comprising: the mixing component is connected with the bottom of the container; the mixing power piece is connected with the mixing assembly; wherein the mixing assembly is configured to store gas entering through the container, and the mixing power member is configured to inject gas into the container through the bottom of the container through the mixing assembly to mix the reagent and the sample to be measured.

Another aspect of an embodiment of the present application also provides a sample analyzer, including: a housing; a blood routine functional module disposed within the housing; a specific protein detection module disposed within the housing and including a measurement system as described above for measurement analysis of a specific protein.

Compared with the prior art, the measuring system provided by the application has the advantages that the mixing component is connected with the bottom of the container, gas is directly stored in at least part of the mixing component through the container, an additional gas acquisition device is not needed, the overall layout of the measuring system is simplified, the overall layout of the measuring system is more compact, the space is saved, and the production cost is reduced. The mixing power piece is further controlled to inject gas into the container from the bottom of the container through the mixing assembly, so that the gas rises from bottom to top, bubbles rise under the action of buoyancy, turbulence is formed locally, and then the sample to be measured and the reagent are fully mixed, so that the sample to be measured and the reagent fully react, and the measurement accuracy is effectively improved. Meanwhile, the gas is directly injected from the bottom of the container through the bubble mixing pipeline unit in the mixing assembly, so that the sample to be tested and the reagent react in the container all the time, and therefore, the problem that the sample to be tested and the reagent react to generate an insoluble compound which is easy to adhere to the inner wall of the sucking and spitting mixing pipeline is avoided, the risk of pipeline blockage or carrying pollution caused by the mixing process is reduced, and the cleaning difficulty is reduced.

Further, in the measuring system, when the sample to be measured and the first reagent are uniformly mixed, the mixing assembly is connected with the bottom of the container, so that the mixing power piece is controlled to inject gas into the container through the bottom of the container by the mixing assembly, and the sample to be measured and the first reagent are fully mixed to form a first mixed solution. And then, sucking part of the first mixed liquid from the container through the back suction device, emptying the first mixed liquid based on the rest of the first mixed liquid in the container, adding part of the first mixed liquid, the second reagent and the third reagent sucked in the back suction device into the container, and injecting gas into the container through the bottom of the container through the mixing component by controlling the mixing power piece, so that the first mixed liquid is fully mixed with the second reagent and the third reagent to form a second mixed liquid. Therefore, the consumption of each reagent can be saved during measurement, and the measuring system of the application injects gas into the container from the bottom of the container for many times through the mixing component, so that the gas rises from bottom to top, the gas is beneficial to rising under the action of buoyancy, turbulence is formed locally, and then the sample to be measured is fully and uniformly mixed with the first reagent, the second reagent and the third reagent, so that the sample to be measured reacts with the reagents more fully, and the measuring accuracy is further improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a measurement system according to an embodiment of the present application;

FIG. 2 is a schematic diagram of a measurement system according to another embodiment of the present application;

FIG. 3 is a schematic diagram of a measurement system according to another embodiment of the present application;

FIG. 4 is a schematic diagram of a measurement system according to another embodiment of the present application;

FIG. 5 is a schematic diagram of a measurement system according to another embodiment of the present application;

FIG. 6 is a schematic diagram of a measurement system according to another embodiment of the present application;

FIGS. 7a to 7f are schematic diagrams illustrating the operation of step S1 in the method of operating the measurement system of the embodiment of FIG. 1;

FIGS. 8a to 8e are schematic views showing an operation procedure of step S1 in the operation method of the measuring system in the embodiment shown in FIG. 4;

FIGS. 9a to 9e are schematic views showing an operation procedure of step S1 in the operation method of the measuring system in the embodiment shown in FIG. 5 or FIG. 6;

FIG. 10 is a schematic diagram of one operational procedure of step S3 in the method of operation of the measurement system of the embodiment of FIG. 1;

FIG. 11 is a schematic diagram of another operation of step S3 in the method of operation of the measurement system of the embodiment of FIG. 1;

FIG. 12 is a schematic diagram of the operation of step S3 in the method of operation of the measurement system of the embodiment of FIG. 4;

FIG. 13 is a schematic diagram of the operation of step S3 in the method of operation of the measurement system of the embodiment of FIG. 5;

FIG. 14 is a schematic diagram of one operational procedure of step S4 in the method of operation of the measurement system of the embodiment of FIG. 1;

fig. 15 is a schematic structural view of a sample analyzer according to an embodiment of the present application.

Detailed Description

The application is described in further detail below with reference to the drawings and examples. It is specifically noted that the following examples are only for illustrating the present application, but do not limit the scope of the present application. Likewise, the following examples are only some, but not all, of the examples of the present application, and all other examples, which a person of ordinary skill in the art would obtain without making any inventive effort, are within the scope of the present application.

The terms "first," "second," "third," and the like in this disclosure are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", and "a third" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise. All directional indications (such as up, down, left, right, front, back … …) in the embodiments of the present application are merely used to explain the relative positional relationship, movement, etc. between the components in a particular gesture (as shown in the drawings), and if the particular gesture changes, the directional indication changes accordingly. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.

Measurement system 100

Referring to fig. 1, fig. 1 is a schematic diagram illustrating a measurement system 100 according to an embodiment of the application. The measurement system 100 may be applied to blood routine detection modules and/or specific protein detection modules. Specifically, as shown in fig. 1, the measurement system 100 may include: a container 10; sample supply means 20 for feeding a sample to be measured into container 10; a reagent supply configured to supply a reagent and add the reagent to the container 10; and a mixing device 60 attached to the bottom of the container 10. Wherein, mixing device 60 includes: a mixing assembly 61 connected to the bottom of the container 10, and a mixing power member 62 connected to the mixing assembly 61, and the mixing assembly 61 stores gas in at least part of the mixing assembly 61 via the container 10, and the mixing power member 62 is further configured to inject gas into the container 10 via the mixing assembly 61 via the bottom of the container 10 to thoroughly mix the reagent and the sample to be measured.

In some embodiments, the reagent supply device may include: a first reagent supplying means 30 for supplying a first reagent and adding the first reagent to the container 10 to form a first mixed solution with the sample to be measured; and a second reagent supply means 40 for supplying a second reagent and adding the second reagent to the container 10; and third reagent supplying means 50 for supplying a third reagent and adding the third reagent to the container 10.

Specifically, after the sample to be tested and the first reagent are added into the container 10, the mixing power member 62 is controlled to inject the gas from the bottom of the container 10 through the mixing assembly 61, so that the sample to be tested and the first reagent are fully mixed to form the first mixed solution. Further, after the second reagent and the third reagent are added into the container 10, the gas is injected from the bottom of the container 10 through the mixing assembly 61 by controlling the mixing power member 62, so that the first mixed solution is fully mixed with the second reagent and the third reagent to form a second mixed solution. It is understood that the first mixed solution may also be referred to as an initial mix sample, and the second mixed solution may also be referred to as a mix sample or mix solution to be measured.

Alternatively, the sample to be measured may comprise a blood sample to be measured.

In some embodiments, the measurement system 100 may also include a suck-back device (not shown). Wherein, after the sample to be tested is fully and uniformly mixed with the first reagent, the measuring system can suck part of the first mixed solution from the container 10 through the back suction device. Further, based on the suck-back means sucking back part of the first mixed liquor, the remaining first mixed liquor in the vessel 10 is emptied. Upon emptying the container 10 of the remaining first mixed liquor, the suck-back device may also re-add a portion of the first mixed liquor to the container 10 to mix with the second reagent, the third reagent to form a second mixed liquor.

When part of the first mixed solution, the second reagent and the third reagent are added into the container 10, the gas can be injected from the bottom of the container 10 through the mixing assembly 61 by controlling the mixing power piece 62, so that part of the first mixed solution, the second reagent and the third reagent are fully mixed to form the second mixed solution.

Container 10

Referring again to fig. 1, the vessel 10 may be a vessel 10 that contains a liquid and provides a reaction site for the liquid. Alternatively, the basic structure of the container 10 may refer to the prior art. Alternatively, the vessel 10 may also be used as a measuring vessel 10 for directly measuring the liquid after the reaction. For example, the vessel 10 may be a reaction cell or a detection cell.

Specifically, the side walls and the bottom wall of the container 10 may be provided with connectors. Wherein the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40 may be connected to the container 10 through connection ports. Alternatively, the third reagent supplying apparatus 50 may be connected to the container 10 through a connection port. In other embodiments, the side and/or bottom walls of the container 10 may not include connectors for reagent supply, and an opening may be provided at the top of the container 10. At this time, the first reagent supplying apparatus 30, the second reagent supplying apparatus 40, and the third reagent supplying apparatus 50 may add reagents into the container 10 through the openings, respectively; at this time, the first reagent supplying apparatus 30, the second reagent supplying apparatus 40, and the third reagent supplying apparatus 50 do not need to be connected to the container 10.

In some embodiments, the inner wall of the container 10 may use a material that is well hydrophobic, such as a teflon material. The design of the container 10 facilitates the liquid in the container 10 to be easily cleaned when the liquid in the container 10 is emptied, reduces the residual waste liquid on the inner wall of the container 10, and further reduces the cleaning difficulty of the container 10 and the maintenance frequency of the measuring system 100.

Sample supply device 20

As shown in fig. 1, the sample supply 20 may include a sample barrel 21, a sampling needle assembly 22, and a sampling power member (not shown). Wherein the sample barrel 21 is configured to hold a sample to be tested. The sampling power member communicates with the sampling needle assembly 22 and provides power for the sampling needle assembly 22 to draw in and expel fluid, thereby driving the sampling needle assembly 22 to draw in a sample to be measured from the sample barrel 21 and transfer the sample to be measured to the container 10.

Alternatively, the sampling power member may be a syringe, a fixed displacement pump, or a positive and negative pressure source, so long as it is capable of powering the aspiration and expulsion of fluid from the needle assembly 22.

Further, the needle assembly 22 may include a needle, a predetermined track (not shown) and a needle power member (not shown). Wherein the sampling needle power member may drive the sampling needle to move along a predetermined trajectory to a corresponding position such that the sampling needle enters or exits the corresponding container 10. Alternatively, the specific structure of the sampling needle may refer to the prior art, and the application is not limited thereto.

First reagent supplying apparatus 30

In some embodiments, the first reagent supplying apparatus 30 may include a first reagent tank 31 containing a first reagent, a first driving member 32 connected with the first reagent tank 31, and a first pipe unit. Wherein the first piping unit is connected between the container 10 and the first driving member 32. In other embodiments, the first reagent supplying apparatus 30 may further include other connecting lines, such as a first line T1, a second line T2, and a valve SV1, which connect the first reagent tank 31 with the first driving member 32. For convenience of description, other connection lines of the first reagent supplying apparatus 30 are defined as first connection lines in the following description of the present application. It is to be understood that the first connection pipe includes, but is not limited to: the first pipeline T1, the second pipeline T2, and the valve SV1 may further be added or subtracted according to the specific application, which is not particularly limited in the present application. Specifically, as shown in fig. 1, the first driving member 32 includes a first opening 321 and a second opening 322, and the first opening 321 may be connected with the first reagent vessel 31 through a first connection line, the first line unit being connected between the container 10 and the second opening 322 of the first driving member 32. Specifically, a second pipeline T2, a valve SV1 and a first pipeline T1 are sequentially connected between the first opening 321 and the first reagent bucket 31; the second opening 322 is connected to the container 10 through a first pipe unit, and specifically, the first pipe unit includes at least a fifth pipe T5 connected to the second opening 322, a ninth pipe T9 connected to the container 10, and a valve SV3 connecting the fifth pipe T5 and the ninth pipe T9. Further, the first driving member 32 sucks the first reagent from the first reagent vessel 31 through the first connecting line and adds the first reagent to the container 10 through the first line unit to mix with the sample to be measured in the container 10 to form a first mixed solution.

In particular, the first reagent comprises a sample diluent, such as a hemolysis agent. The sample diluent can be used for dissolving blood cells in a sample to be detected, so that the sample to be detected can be detected by using an in-vitro diagnostic reagent or instrument.

Alternatively, the first driving member 32 is a hydrodynamic device, specifically, a syringe, a dosing pump, a syringe pump, a positive and negative pressure source, or the like, for applying pressure to the liquid therein, or the liquid in the first reagent vessel 31, to suck the first reagent in the first reagent vessel 31 or to add the sucked first reagent into the container 10. Of course, the first driving member 32 may be any other hydrodynamic device capable of outputting a constant pressure. The present application is described below in terms of the first drive member 32 being a syringe.

It will be appreciated that, while the first drive member 32 is one of a syringe, a fixed displacement pump, a syringe pump, a positive and negative pressure source, etc., the specific configuration may be any other known configuration, and is not limited in this disclosure.

In other embodiments, the first reagent supply device 30 may further include a first pre-heater 33 in order to pre-heat the first reagent before being added to the container 10. Wherein one end of the first preheating piece 33 is connected with the second opening 322, and the other end of the first preheating piece 33 is connected with the container 10. At this time, the first pipe unit further includes a seventh pipe T7 connecting the first preheating part 33 with the valve SV 3.

Optionally, the first pre-heating element 33 is part of the container 10 or pipe in which the liquid can be held and is provided with a heating element. The first reagent sucked up by the first driving part 32 is introduced into the first preheating part 33 through the fifth line T5, and the first preheating part 33 may preheat the first reagent contained therein.

Specifically, as shown in fig. 1, the first opening 321 of the first driving member 32 is connected to the first reagent tank 31 through the second pipeline T2, the valve SV1, and the first pipeline T1 to suck the first reagent. Further, one end of the first pre-heating member 33 is connected to the second opening 322 of the first driving member 32 through the fifth pipe T5, and the other end of the first pre-heating member 33 is connected to the container 10 through the seventh pipe T7, the valve SV3, and the ninth pipe T9, so that the first driving member 32 can pre-heat the sucked first reagent through the first pre-heating member 33 before adding it to the container 10, and then add the pre-heated first reagent to the container 10 through the ninth pipe T9. The preheated first reagent is added into the container 10 and then fully and uniformly mixed with the sample to be tested in the container 10 through the uniformly mixing device 60, so as to obtain a first mixed sample liquid.

In some embodiments, in the mixing process of the sample to be measured and the preheated first reagent, in order to avoid heat dissipation of the liquid, a heating element (not shown) may be disposed on the outer wall of the container 10, so as to ensure that the temperature in the mixing process is constant, and further improve the accuracy of the detection result.

In some embodiments, the fifth line T5, the seventh line T7, the valve SV3, and the ninth line T9 may form a first line unit.

In other embodiments, the fifth line T5, the seventh line T7, the first pre-heater 33, the valve SV3, and the ninth line T9 may form a first line unit.

It will be appreciated that the first piping unit includes, but is not limited to, piping and valves as described in the above embodiments, such as: the fifth pipeline T5, the seventh pipeline T7, the valve SV3 and the ninth pipeline T9 may further add or subtract pipelines and/or valves according to the specific application, and the present application is not limited in particular.

Alternatively, valves SV1 and SV3 may be two-way valves.

Before the first reagent is added into the container 10, the first reagent is preheated by the first preheating piece 33, and then the preheated first reagent is added into the container 10, so that a temperature environment simulating an organism is provided for a sample to be detected, and the accuracy of a detection result is improved.

It can be understood that the order of addition of the sample to be tested and the first reagent in the embodiment of the present application is not sequential. Alternatively, the sample to be tested in the embodiments of the present application may be added to the container 10 before the first reagent is added, after the first reagent is added, or both.

In some embodiments, the order of addition of the two may also be as follows: a portion of the first reagent may be added to the container 10 as a base solution, then the sample to be measured is added to the container 10, and mixed with the first reagent, then the remaining portion of the first reagent is added to the container 10, and then the gas is injected into the container 10 from the bottom of the container 10 through the mixing assembly 61 by controlling the mixing power member 62, so as to sufficiently measure the sample and the first reagent. Therefore, part of the base solution is added into the container 10 in advance, so that the sample to be measured can be conveniently dissolved and diffused into the first solvent when being punched out from the sampling needle, and is not suspended on the needle tip of the sampling needle, and the rest part of the reagent is continuously added into the container 10, so that the mixing is facilitated. Moreover, this mixing method is particularly suitable for cases where the amount of the sample to be measured is small, for example, 2 to 8ul of the sample to be measured.

Second reagent supplying apparatus 40

Referring to fig. 1 again, the second reagent supplying apparatus 40 may include a second reagent tank 41 for holding a second reagent, a second driving member 42 connected with the second reagent tank 41, and a second pipe unit. Wherein the second piping unit is connected between the container 10 and the second driving member 42. In other embodiments, the second reagent supplying apparatus 40 may further include other connecting lines, such as a third line T3, a fourth line T4, and a valve SV2, which connect the second reagent tank 41 with the second driving member 42. For convenience of description, other connection lines of the second reagent supplying apparatus 40 are defined as second connection lines in the following description of the present application. It is understood that the second connecting line includes, but is not limited to: the third pipeline T3, the fourth pipeline T4 and the valve SV2 may further be added or subtracted according to the specific application, which is not particularly limited in the present application.

Specifically, as shown in fig. 1, the second driving part 42 includes a third opening 421 and a fourth opening 422, and the third opening 421 may be connected with the second reagent vessel 41 through a second connection line, specifically, a third line T3, a valve SV2, and a fourth line T4 are sequentially connected between the third opening 421 and the second reagent vessel 41; the fourth opening 422 is connected to the container 10 by a second piping unit, namely: the second pipe unit is connected between the container 10 and the fourth opening 422 of the second driving member 42. Specifically, the second pipe unit includes at least a sixth pipe T6 connected to the fourth opening 422, a tenth pipe T10 connected to the container 10, and a valve SV4 connecting the sixth pipe T6 and the tenth pipe T10. Further, the second driving member 42 serves to suck the second reagent and add the second reagent to the container 10 through the second pipe unit.

Specifically, the second reagent comprises a buffer. The buffer solution can be used for providing a specific environment for the diluted sample to be tested to react with the third reagent. Specifically, the sample to be tested in the container 10 is reacted with the first reagent, and after the sample to be tested is mixed with the first reagent to form a first mixed solution, a third reagent is added to react with the first mixed solution to generate antigen-antibody reaction. When the third reagent reacts with the first mixed solution, a buffer solution is needed to provide the physiological environment required by the reaction.

Similarly, the second driving member 42 may also be a hydrodynamic device, and its specific structure is the same as or similar to that of the first driving member 32, and will not be described in detail. The present application is described below in terms of the second drive member 42 being a syringe.

In other embodiments, the second reagent supply device 40 may further include a second preheating member 43 in order to perform a preheating process of the second reagent before being added to the container 10. Wherein one end of the second preheating piece 43 is connected with the fourth opening 422, and the other end of the second preheating piece 43 is connected with the container 10. At this time, the second pipe unit further includes an eighth pipe T8 connecting the second preheating piece 43 with the valve SV 4.

Similarly, the specific structure of the second preheating piece 43 is substantially the same as that of the first preheating piece 33, and will not be described in detail.

Specifically, as shown in fig. 1, one end of the second driving member 42 is connected to the second reagent tank 41 through the third pipe T3, the valve SV2, and the fourth pipe T4 to suck the second reagent in the second reagent tank 41. Further, one end of the second preheating part 43 is connected to the fourth opening 422 of the second driving part 42 through the sixth pipeline T6, and the other end of the second preheating part 43 is connected to the container 10 through the eighth pipeline T8, the valve SV4, and the tenth pipeline T10, so that the second driving part 42 may perform the preheating treatment through the second preheating part 43 before adding the sucked second reagent to the container 10, and then add the preheated second reagent to the container 10 through the tenth pipeline T10.

In some embodiments, the sixth tubing T6, eighth tubing T8, valve SV4, and tenth tubing T10 may form a second tubing unit.

In other embodiments, the sixth line T6, the eighth line T8, the second pre-heater 43, the valve SV4, and the tenth line T10 may form a second line unit.

It will be appreciated that the second piping unit includes, but is not limited to, piping and valves as described in the above embodiments, such as: the sixth pipeline T6, the eighth pipeline T8, the valve SV4, and the tenth pipeline T10 may further add or subtract pipelines and/or valves according to the specific application, and the present application is not limited thereto.

Similarly, valve SV2 and valve SV4 may also be two-way valves.

Before the second reagent is added into the container 10, the second reagent is preheated by the second preheating piece 43, and then the preheated second reagent is added into the container 10, so that a temperature environment simulating an organism is provided for a sample to be detected, and the accuracy of a detection result is improved.

In the specific implementation process, before the start of the operation, the pipelines in the first reagent supply device 30 are filled with the first reagent, namely: the first pipeline T1, the second pipeline T2 and the first pipeline unit are all filled with the first reagent. Similarly, the lines in the second reagent supply 40 are also filled with the second reagent, namely: the third pipeline T3, the fourth pipeline T4 and the second pipeline unit are all filled with the second reagent.

At the beginning of the operation, by controlling the opening and closing sequence of the valve and the suction state of the first driving member 32, the first driving member 32 sucks the first reagent, injects the sucked first reagent into the first preheating member 33 through the fifth pipeline T5 for preheating, then adds the preheated first reagent into the container 10 through the seventh pipeline T7 and the ninth pipeline T9, and then fully mixes the preheated first reagent with the sample to be measured in the container 10 through the mixing device 60 to obtain the first mixed solution.

Similarly, when the second reagent is required to be added, the preheated second reagent can be added to the container 10 by controlling the opening and closing sequence of the valve and the suction state of the second driving member 42.

It will be appreciated that although fig. 1 shows the first reagent supplying apparatus 30 being connected to the container 10 via the ninth pipe T9, the second reagent supplying apparatus 40 being connected to the container 10 via the tenth pipe T10, this is not essential. That is, the positional relationship of the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40 is not necessarily completely as shown in the drawings, for example: the first reagent supply means 30 may also be arranged to add a first reagent through the tenth conduit T10, or the second reagent supply means 40 may also be arranged to add a second reagent through the ninth conduit T9. The first reagent vessel 31 and the second reagent vessel 41 are disposed at the corresponding positions as needed, and are not particularly limited herein.

First reagent supply device 30 and second reagent supply device 40-sampling needle structure

In other embodiments, the first reagent supply 30 and the second reagent supply 40 may be part of a similar structure to the sample supply 20, using the same set of sampling needle assemblies 22 and sampling power members or more than two sets of sampling needle assemblies 22 and sampling power members, to add the first reagent and the second reagent to the container 10, respectively, without being connected to the container 10 by connecting lines.

Specifically, the first reagent supplying apparatus 30 may also include: a first reagent cartridge 31 for holding a first reagent, a sampling needle assembly 22 for aspirating the first reagent, and a sampling power member. Wherein a sampling power member is in communication with the sampling needle assembly 22, the sampling power member driving the sampling needle assembly 22 to draw a first reagent from the first reagent cartridge 31 and driving the sampling needle assembly 22 to add the drawn first reagent to the container 10.

Specifically, the second reagent supplying apparatus 40 may also include: a second reagent cartridge 41 for holding a second reagent, a sampling needle assembly 22 for aspirating the second reagent, and a sampling power member. Wherein a sampling power member is in communication with the sampling needle assembly 22, the sampling power member driving the sampling needle assembly 22 to draw a second reagent from the second reagent tank 41 and driving the sampling needle assembly 22 to add the drawn second reagent to the container 10.

Third reagent supply device 50-sampling needle structure

In some embodiments, the third reagent supply 50 may also be part of a structure similar to the sample supply 20, and the sampling needle assembly 22 may be used to add the third reagent to the container 10 without being connected to the container 10 by a connecting line.

Specifically, the third reagent supplying apparatus 50 may also include: a third reagent cartridge 51 for holding a third reagent, a sampling needle assembly 22 for aspirating the third reagent, and a sampling power member. Wherein the sampling power member drives the sampling needle assembly 22 to draw the third reagent from the third reagent cartridge 51 and drives the sampling needle assembly 22 to add the drawn third reagent to the container 10.

In some embodiments, the third reagent comprises a latex reagent and is configured to react with the first mixed liquor to produce an antigen-antibody reaction.

The operation principle of the sampling needle assembly 22 and the sampling power member of the third reagent supplying apparatus 50 is substantially the same as that of the sampling needle assembly 22 and the sampling power member of the sample supplying apparatus 20. Similarly, the specific structure of the sampling needle assembly 22 and the sampling power member can also be referred to as the sampling needle assembly 22 and the sampling power member in the sample supply device 20, and will not be described herein.

It is understood that in the measurement system of the present application, the first reagent supplying apparatus 30 and/or the second reagent supplying apparatus 40 and/or the third reagent supplying apparatus 50 and/or the sample supplying apparatus 20 may be configured by the sampling needle assembly 22 and the sampling power member. Specifically, the sampling power member communicates with the sampling needle assembly 22 to draw the corresponding reagent from the reagent cartridge or the sample to be measured from the sample cartridge 21 through the sampling needle assembly 22 and to add the drawn reagent or the drawn sample to be measured to the container 10 through the sampling needle assembly 22. The reagent tanks may include the first reagent tank 31, the second reagent tank 41, and the third reagent tank 51 as described in the above embodiments. The reagents that are aspirated may include a first reagent, a second reagent, and a third reagent as described in the previous examples.

Third reagent supplying apparatus 50-pipeline structure

In other embodiments, the third reagent supplying apparatus 50 may also employ a pipeline structure of the first reagent supplying apparatus 30 or the second reagent supplying apparatus 40 to add the third reagent into the container 10. For example, the third reagent may be added to the container 10 by sharing the second driving member 42 and part of the connecting line with the second reagent supplying apparatus 40, also by the line structure.

Referring to fig. 2, fig. 2 is a schematic diagram illustrating a measurement system according to another embodiment of the application.

Specifically, as shown in fig. 2, the third reagent supplying apparatus 50 may include a third reagent vessel 51, a second driving member 42, and corresponding connection lines. In some embodiments, valve SV4 in the second reagent supply 40 is set as a three-way valve. The valve SV4 is connected to the second driver 42 via a valve SV9 and an eighth line T8, to the container 10 via a tenth line T10, and to the third reagent vessel 51 via a twenty-first line T21, respectively.

In a specific implementation process, the second driving member 42 can respectively add the second reagent and the third reagent into the container 10 by controlling the opening and closing sequence of the valve and the suction state of the second driving member 42. Specifically, when it is necessary to add the second reagent to the container 10, the valves SV4, SV9 and SV2 are controlled so that the connection lines between the second reagent tank 41, the second driver 42, the second pre-heater 43 and the container 10 are in a communication state, and the twenty-first line T21 is in a disconnected state, the second reagent is sucked through the second driver 42 and added to the container 10. When it is desired to add the third reagent to the container 10, the valves SV4, SV9 and SV2 are controlled so that the connection lines between the second driver 42, the second pre-heater 43 and the third reagent tank 51 are in a connected state, and so that the connection line of the second reagent tank 41 and the second driver 42 is in a disconnected state (i.e., the valve SV2 is disconnected from the third line T3), the third reagent is sucked through the second driver 42 and added to the container 10.

The present application not only realizes the supply of two different reagents, but also simplifies the design of the pipeline by sharing the second driving member 42 and part of the connecting pipeline with the second reagent supplying apparatus 40 and the third reagent supplying apparatus 50.

It will be appreciated that, between the tenth pipeline T10, the twenty-first pipeline T21 and the valve SV9, the three-way valve SV4 is not provided, but the three-way pipe is directly used for connection, and at the same time, one two-way valve is provided on each of the tenth pipeline T10 and the twenty-first pipeline T21, so long as it can ensure that the open/close states of the valves are controlled during operation, so that the second driving member 42 and the third reagent tank 51, and the second driving member 42 and the container 10 can be independently in the communicating or closing state. Namely, the third reagent vessel 51, the container 10, and the second driver 42 are connected.

It will be appreciated that reference may be made to the operation of the first reagent supplying apparatus 30 or the second reagent supplying apparatus 40 in the above embodiment as to how the second reagent and the third reagent are respectively preheated before being added to the container 10.

The first reagent supplying apparatus 30 and the second reagent supplying apparatus 40 share a common pipeline

To further simplify the construction of the measurement system 100, the present application provides another construction design of the measurement system 100. Specifically, as shown in fig. 3, fig. 3 shows a schematic structural diagram of a measurement system 100 according to still another embodiment of the present application.

It should be noted that the structural design of the measurement system 100 shown in fig. 3 is mainly different from that of fig. 1 in that the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40, while the structural design of other elements is substantially the same as that of the embodiment shown in fig. 1, and reference may be made to the embodiment shown in fig. 1. The first reagent supplying apparatus 30 and the second reagent supplying apparatus 40 shown in FIG. 3 are described in detail below.

Referring to fig. 1 and 3 in combination, the measuring system 100 in fig. 3 is mainly different from the measuring system 100 in fig. 1 in that the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40 share one driving member and one preheating member, and share part of the connecting line. For example, the first drive member 32 or the second drive member 42 shown in FIG. 1, the drive member and/or the preheat member that are common to both, are not limiting in this application. That is, the structural design in fig. 1 can be adjusted according to the design requirement of the common structure in the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40, and the common or same element structures thereof are the same as described above. The present embodiment will be described by taking the example in which the first driving member 32 and the first preheating member 33 are shared by both.

Specifically, as shown in fig. 3, the first reagent supplying apparatus 30 includes a first driving part 32, a first reagent vessel 31 connected to a second opening 322, and a third pipe unit, and the second reagent supplying apparatus 40 includes a first driving part 32, a second reagent vessel 41 connected to a first opening 321, and a third pipe unit. Further, the valve SV3 in the first reagent supply device 30 is set to a three-way valve. The valve SV3 is connected to the first drive 32 via a fourth line T4, to the first reagent vessel 31 via a fifth line T5, and to the container 10 via a sixth line T6. Specifically, a second pipeline T2, a valve SV1 and a first pipeline T1 are sequentially connected between the first opening 321 and the second reagent tank 41; the second opening 322 is connected to the container 10 by a third piping unit. Wherein the third pipeline unit at least comprises: a third pipe T3 connected to the second opening 322, a fourth pipe T4 connected to the third pipe T3, a sixth pipe T6 connected to the fourth pipe T4 through a valve SV3, a seventh pipe T7 connecting the sixth pipe T6 and the container 10, a valve SV2 connecting the third pipe T3 and the fourth pipe T4, and a valve SV3.

It will be appreciated that the third piping unit includes, but is not limited to, piping and valves as described in the above embodiments, such as: the third pipeline T3, the fourth pipeline T4, the sixth pipeline T6, the seventh pipeline T7, the valve SV2 and the valve SV3 may further be added or deleted according to the specific application, and the present application is not limited in particular. In a specific implementation process, the first driving member 32 can respectively add the first reagent and the second reagent into the container 10 by controlling the opening and closing sequence of the valve and the suction state of the first driving member 32. Specifically, when the first reagent needs to be added to the container 10, the valves SV1, SV3 and SV2 are controlled to connect or disconnect the connection lines in the first reagent supply device 30, that is: first, the valve SV1 is closed, the valve SV3 and the valve SV2 are opened, wherein the opened valve SV3 is connected to the fourth pipe T4 and the fifth pipe T5, respectively, at this time, the connection pipe (i.e., the third pipe T3, the fourth pipe T4 and the fifth pipe T5) between the first reagent vessel 31, the first driving member 32 and the container 10 is in a connected state, and the connection pipe between the first driving member 32 and the second reagent vessel 41 is in a disconnected state. Thereby, the first driving member 32 sucks the first reagent from the first reagent vessel 31 through the third pipe T3, the valve SV2, the fourth pipe T4, the valve SV3, and the fifth pipe T5 in this order. Then, the valve SV2 is continuously opened while the valve SV3 is controlled, that is: the valve SV3 is switched to be connected to the fourth and sixth lines T4, T6, respectively, such that the fifth line T5 is in a disconnected state from the fourth line T4 and the fourth line T4 is in communication with the sixth and seventh lines T6, T7, whereby the first driver 32 can add the sucked first reagent to the container 10 through the third, fourth, sixth and seventh lines T3, T4, T6, T7.

When it is desired to add the second reagent to the container 10, the valves SV1, SV3 and SV2 are controlled to connect or disconnect the connecting lines in the second reagent supply device 40, that is: first, the valve SV1 is opened, and simultaneously, the valve SV3 and the valve SV2 are closed, at this time, the connecting line between the second reagent vessel 41 and the first driving member 32 is in a communicating state, and the connecting line between the first driving member 32 and the first reagent vessel 31 is in a disconnecting state (i.e., the valve SV3 is disconnected from the fifth line T5). Thereby, the first driving member 32 sucks the second reagent from the second reagent vessel 41 through the second pipe T2, the valve SV1, and the first pipe T1 in this order. Then, the valve SV1 is closed, and at the same time, the valve SV3 and the valve SV2 are opened, wherein the valve SV3 is connected to the fourth line T4, the sixth line T6, respectively, such that the fifth line T5 is in a disconnected state from the fourth line T4 and the fourth line T4 is in communication with the sixth line T6, the seventh line T7, whereby the first driver 32 can add the sucked second reagent to the container 10 through the third line T3, the fourth line T4, the sixth line T6, and the seventh line T7.

In some embodiments, a first pre-heater 33 is further provided between the sixth and seventh lines T6, T7 to add the pre-heated first and second reagents, respectively, to the container 10. The operation principle and structure of the first preheating piece 33 are the same as in the above embodiment.

It will be appreciated that when the first and second reagent supplying apparatuses 30 and 40 employ a common driving member, a preheating member and a part of the connection lines, the third line T3, the valve SV2, the fourth line T4, the valve SV3, the sixth line T6 and the seventh line T7 may form a third line unit in the first/second reagent supplying apparatus 40.

By sharing the driving member, the preheating member and a part of the connecting line with the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40, the connecting line can be further reduced, the structure can be simplified, and the equipment cost can be saved.

It should be noted that, although the order of addition of the first reagent and the second reagent in the above embodiments is separately added to the container 10, that is: a sufficient amount of the first reagent is first aspirated into the container 10 and then a sufficient amount of the second reagent is aspirated into the container 10, although this is not required. In other embodiments, the first and second reagents may also be spaced apart in a pipeline, with the specific operation being as follows.

Before the start of the operation, the liquid arranged at intervals can be stored in the fourth line T4 by controlling the opening and closing sequence of the valves SV1, SV2, SV3 and the state of sucking the liquid by the first driving element 32.

In one embodiment, the valve SV1 is opened, the valve SV2 is opened, the valve SV3 is connected to the fourth and sixth pipelines T4 and T6, respectively, the second reagent in the sixth and seventh pipelines T6 and T7 is sucked by the first driver 32 and sucked back to the fourth pipeline T4 while sucking part of the air; then, the valve SV3 is switched to be connected to the fourth pipeline T4 and the fifth pipeline T5 respectively, the states of the valve SV1 and the valve SV2 are unchanged, and the first driving element 32 stores the sucked first reagent in the fourth pipeline T4; finally, the valve SV2 is closed, the valve SV1 is opened, and the second reagent is sucked by the first driving member 32, so that a section of the first reagent and a section of the second reagent are arranged in the fourth pipeline T4, the middle is separated by air, and the distance between the first reagent and the container 10 is shorter than the distance between the second reagent and the container 10 in the pipeline.

In another embodiment, first, valve SV1 is opened, valve SV2 is opened, and the first driver 32 aspirates the second reagent and stores it in the body of the first driver 32; valve SV1 is then opened, valve SV2 and valve SV3 are opened, valve SV3 is now connected to T4, T5, respectively, and first driver 32 draws the first reagent into fourth line T4.

At the beginning of the operation, a length of the first reagent stored in the fourth line T4 is preheated by the first preheating means 33 and added to the container 10 by the seventh line T7. When it is desired to inject the second reagent into the container 10, a length of the second reagent, which has been stored in the body of the first driving member 32, is preheated by the first preheating member 33 and is added to the container 10 through the seventh line T7.

It will be appreciated that, between the fifth pipeline T5, the fourth pipeline T4 and the sixth pipeline T6, the three-way valve SV3 is not provided, but a three-way pipe is directly used for connection, and two-way valves are provided on the pipeline T6 and the pipeline T5, so long as it can ensure that the first driving member 32 and the first reagent vessel 31, and the first driving member 32 and the container 10 can be independently in a communication state or a closed state by controlling the opening and closing states of the valves during operation. Namely, the first reagent vessel 31, the container 10 and the second opening of the first driver 32 are connected.

It will be appreciated that although fig. 3 shows the first reagent supply device 30 and the second reagent supply device 40 in common with the first driving member 32 and the first pre-heating member 33, this is not required. In some embodiments, the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40 may also share the second driving member 42 and the second preheating member 43 shown in fig. 1, and the working principle thereof is substantially the same as that of the first driving member 32 and the first preheating member 33, which are referred to above and will not be described herein.

In other embodiments, the reagent supply device may include: a first reagent supply means 30 for supplying a first reagent and adding the first reagent to the container 10; and a third reagent supplying means 50 for supplying a third reagent and adding the third reagent to the container 10. Wherein the first reagent comprises a mixture of a hemolytic agent and a buffer, and the third reagent comprises a latex antibody. The structures of the first reagent supplying apparatus 30 and the third reagent supplying apparatus 50 may refer to the above-mentioned related embodiments, and are not described herein.

Specifically, the mixing power member 62 is controlled to inject gas into the container 10 from the bottom of the container 10 through the mixing assembly 61 based on the mixed solution of the hemolysis agent and the buffer solution, the sample to be tested and the latex antibody into the container 10, so as to sufficiently mix the hemolysis agent and the buffer solution, the sample to be tested and the latex antibody and form the mixed solution to be tested.

Mixing device 60

In the process of performing detection analysis on a sample to be detected, various reagents required for the sample to be detected and its detection process are added to the container 10 through the sample supply device 20 and the reagent supply device, respectively, for example: the first reagent, the second reagent, the third reagent and the like in the application are used for forming a mixed solution to be tested. However, the sample to be measured and the reagents added to the container 10 are not sufficiently mixed and are in an uneven state. And the sample to be tested and each reagent are not fully and uniformly mixed to form a mixed solution to be tested, if the mixed solution to be tested is directly tested, the accuracy of the test result is necessarily affected. That is, the measurement of each index is performed only after the sample to be measured and each reagent are sufficiently mixed, and thus the mixing operation is required. The measurement system provided by the application can realize the mixing operation by arranging the mixing device 60, so that the sample to be measured and each reagent are fully mixed, the sample to be measured and the reagent are fully reacted to form the mixed liquid to be measured, and then the mixed liquid to be measured is detected, thereby effectively improving the detection accuracy.

Referring to fig. 1 to 3 in combination with fig. 4 to 6, it should be noted that the schematic structural diagrams of the measuring system shown in fig. 4 to 6 are adjusted based on the structural design of the measuring system shown in fig. 1 to provide other structural designs of the mixing device 60 in the measuring system. Namely: the main difference between the measuring system shown in fig. 4 to 6 and the measuring system shown in fig. 1 is the different structural design of the mixing assembly 61 in the mixing device 60. In some embodiments, the structural design of the blending assembly 61 shown in fig. 4-6 may also be applied to the blending apparatus 60 of the measurement system shown in fig. 2 and 3.

Specifically, as shown in fig. 1 to 6, the mixing apparatus 60 may include a mixing assembly 61 and a mixing power member 62, wherein the mixing assembly 61 is connected to the bottom of the container 10, the mixing power member 62 is connected to the mixing assembly 61, and the mixing assembly 61 is used to store the gas introduced through the container 10, and the mixing power member 62 may inject the gas stored in the mixing assembly 61 into the container 10 through the mixing assembly 61 through the bottom of the container 10. Wherein the mixing assembly 61 is filled with a liquid before the mixing assembly 61 stores the gas in at least a portion of the mixing assembly via the container 10.

In some embodiments, as shown in fig. 1-4, blending assembly 61 includes at least a bubble blending line unit. Wherein, bubble mixing pipeline unit includes at least: valve SV7, including both ends; the first passage structure comprises a fourteenth pipeline T14, wherein a port of the fourteenth pipeline T14 connected with the uniform mixing power piece 62 is a first port, and a port of the fourteenth pipeline T14 connected with one end of the valve SV7 is a second port; a second passage comprising a fifteenth conduit T15, the fifteenth conduit T15 comprising a third port and a fourth port, the third port being connected to the other end of the valve SV 7; and a third passage including an eighteenth pipeline T18, the eighteenth pipeline T18 including a fifth port and a sixth port, the fifth port being connected to a fourth port of the fifteenth pipeline T15, and the sixth port being connected to the bottom of the container 10; wherein the valve SV7 is configured to control the connection or disconnection of the fourteenth pipe T14 and the fifteenth pipe T15. Alternatively, valve SV7 may be a two-way valve.

In some embodiments, blending assembly 61 may further comprise: a liquid supply source (not shown in the figure) of the bubble mixing line unit, a valve SV8 and corresponding connecting lines. Wherein, corresponding connecting line includes at least: a connecting line provided between the fourteenth line T14 first port and the mixing power element 62, for example: thirteenth line T13, sixteenth line T16 and valve SV8. Specifically, a valve SV8 is provided between the sixteenth pipe T16 and the thirteenth pipe T13 to control the communication or disconnection between the sixteenth pipe T16 and the thirteenth pipe T13. One end of the thirteenth line T13 is connected to the first port of the fourteenth line T14, and the other end of the thirteenth line T13 is connected to one end of the valve SV8. One end of the sixteenth pipe T16 is connected to the other end of the valve SV8, and the other end of the sixteenth pipe T16 is connected to the mixing power element 62.

In some embodiments, the measurement system 100 further comprises: seventeenth line T17 connected to sixteenth line T16 via valve SV 8.

In some embodiments, valve SV8 may be a three-way valve, i.e.: the first end of the valve SV8 is connected to the thirteenth line T13, the second end of the valve SV8 is connected to the seventeenth line T17, and the third end of the valve SV8 is connected to the sixteenth line T16.

It will be appreciated that in some embodiments, the bubble blending line unit may also include the thirteenth line T13, the sixteenth line T16, and the valve SV8 as described above. That is, the connection lines between the thirteenth line T13, the sixteenth line T16, and the fourteenth line T14 may be collectively referred to as a first passage structure of the bubble mixing line unit. Namely: one end of the first passage structure is connected to the mixing power member 62, and the other end of the first passage structure is connected to one end of the valve SV 7. At this time, the valve SV7 may also be configured to control the connection or disconnection of the first passage structure and the fifteenth pipe T15.

In some embodiments, two ends of the first via structure may also be referred to as a first port and a second port, respectively. Wherein a first port of the first passage structure is connected to the mixing power element 62, and a second port of the first passage structure is connected to one end of the valve SV 7.

Further, the liquid supply of the bubble mixing tube unit is connected to the mixing power member 62 through a valve SV8, and the liquid supply of the bubble mixing tube unit can be used to supply liquid to the mixing power member 62, for example: blood cell dilutions. Specifically, when the valve SV8 is switched to connect the sixteenth line T16 with the seventeenth line T17, the mixing power element 62 may draw liquid from the liquid supply of the bubble mixing line unit through the sixteenth line T16 and the seventeenth line T17. Further, the valve SV8 is switched to connect the sixteenth pipeline T16 with the thirteenth pipeline T13, and the control valve SV7 is opened, and the mixing power element 62 is connected to the valve SV7 through the sixteenth pipeline T16, the valve SV8, the thirteenth pipeline T13 and the fourteenth pipeline T14, and further connected to the fifteenth pipeline T15, so that the liquid in the mixing power element 62 is pushed into the fifteenth pipeline T15 of the bubble mixing pipeline unit to supply the liquid into the fifteenth pipeline T15 of the bubble mixing pipeline unit, and the liquid can push out the gas contained in the fifteenth pipeline T15.

Similar to first drive member 32 and/or second drive member 42, blending power member 62 may also be a hydrodynamic device, and may specifically be a syringe, a fixed displacement pump, a syringe pump, or one of a positive and negative pressure source, among others. Preferably, the mixing power element 62 is a syringe. When the mixing power member 62 is a syringe, the bubble velocity of the injected gas can be precisely controlled.

Specifically, as shown in fig. 1 to 6, the mixing power element 62 may include an opening 621 connected with the sixteenth pipe T16. Further, the mixing power member 62 may be used to apply pressure to the liquid in the bubble mixing tube unit to drive the liquid in the bubble mixing tube unit to flow, namely: the mixing power member 62 may provide a first passage structure that drives the bubble mixing tube unit, that is, the sixteenth tube T16, the thirteenth tube T13, and the fourteenth tube T14, and a second passage structure that is, a negative pressure that causes the liquid in the fifteenth tube T15 to flow in a direction away from the isolation chamber 611 or a positive pressure that causes the liquid in the remaining portion of the bubble mixing tube unit to flow in a direction toward the isolation chamber 611. Further, the mixing power element 62 may be further configured to draw liquid from the liquid supply of the bubble mixing tube unit and provide the liquid to the mixing assembly 61 via the corresponding connecting tube. Of course, the mixing power element 62 may be other hydrodynamic devices capable of outputting a constant pressure. The present application is described below in terms of a mixing power element 62 as a syringe.

In some embodiments, as shown in fig. 1-3, blending assembly 61 may further comprise: a separation chamber 611. Wherein the isolation chamber 611 is located between the fifteenth pipe T15 and the eighteenth pipe T18. Specifically, an eighteenth pipe T18 has one end connected to the bottom of the container 10 and the other end connected to one end of the isolation chamber 611. The other end of the isolation chamber 611 is connected to a fifteenth pipe T15. That is, in some embodiments, the mixing assembly 61 is further provided with the isolation chamber 611 based on the structural design of the bubble mixing tube unit at least including the valve SV7, the thirteenth tube T13, the fourteenth tube T14, the fifteenth tube T15 and the eighteenth tube T18.

Specifically, as shown in fig. 1 to 3, the isolation chamber 611 is a closed container, which can be used for holding liquid and storing gas. Further, the isolation chamber 611 is mainly used for gas-liquid separation, and the isolation chamber 611 may be completely emptied of only residual gas or may be in a gas-liquid mixed state. That is, the usage status of the isolation chamber 611 can be adjusted according to specific design requirements.

In some embodiments, the isolation chamber 611 may include a top portion, a bottom portion disposed opposite the top portion, and side edges connected to the top and bottom portions, respectively. The top, bottom and sides together form a closed container. Wherein the top of the isolation chamber 611 communicates with the bottom of the container 10 through an eighteenth pipe T18, and the side of the isolation chamber 611 is connected to a fifteenth pipe T15 of the bubble mixing pipe unit. Specifically, the top of the isolation chamber 611 is connected to the fifth port of the eighteenth pipe T18, and the side of the isolation chamber 611 is connected to the fourth port of the fifteenth pipe T15. Further, the bottom of the isolation chamber 611 may be connected to the evacuation device 80, so that the top is connected to the bottom of the container 10 through the eighteenth pipeline T18, the side is connected to the fifteenth pipeline T15 of the bubble mixing pipeline unit, and the bottom is connected to the isolation chamber 611 of the evacuation device, which can provide conditions for bubble mixing and can be used for buffering the waste liquid. That is, when it is required to inject the gas into the vessel 10 from the bottom of the vessel 10, the mixing power 62 is controlled to inject the gas into the vessel 10 from the bottom of the vessel 10 through the fifteenth pipe T15 of the bubble mixing pipe unit, the side of the isolation chamber 611, the top of the isolation chamber 611, and the eighteenth pipe T18 in this order, whereby the isolation chamber 611 can provide conditions for bubble mixing; when the liquid in the container 10 needs to be discharged, the liquid is discharged from the container 10 through the bottom of the container 10, the eighteenth pipeline T18, the top and bottom of the isolation chamber 611, and the discharge pipeline in this order, and thus the isolation chamber 611 can provide a buffer effect for the discharge of the liquid, namely: the isolation chamber 611 and the eighteenth conduit T18 may cooperate to act as a buffer for the liquid in the container 10 to drain.

In other embodiments, the isolation chamber 611 may also be configured to store gas that enters through the vessel 10. Specifically, after the liquid in the container 10 is emptied by the emptying device 80, the gas can enter the isolation chamber 611 through the container 10, and after the valve of the emptying device 80 is closed, the liquid is added to the container 10 again, and the gas is stored in the isolation chamber 611 because the pipelines at the bottom and the side of the isolation chamber 611 are in the closed state.

Further, when it is required to inject the gas into the vessel 10 from the bottom of the vessel 10, the mixing power member 62 drives the liquid in the bubble mixing pipe unit into the isolation chamber 611 based on the bubble mixing pipe unit being filled with the liquid, thereby compressing the gas stored in the isolation chamber 611, so that the gas in the isolation chamber 611 is compressed. At this time, a part of the gas in the isolation chamber 611 rises along the eighteenth line T18, that is, the gas stored in the isolation chamber 611 is injected into the container 10 through the bottom of the container 10 along the eighteenth line T18.

Please refer to fig. 4 again in combination with fig. 1. Specifically, as shown in fig. 1 and 4, the blending assembly 61 in the measurement system shown in fig. 4 is mainly different from the blending assembly 61 in the measurement system shown in fig. 1 in that: the isolation chamber 611 in the mixing assembly 61 shown in fig. 1 is replaced with the connector 613 shown in fig. 4, and other structures of the mixing assembly 61 shown in fig. 4 are substantially the same as the mixing assembly 61 shown in fig. 1. That is, based on the structural design that the bubble mixing pipe unit at least includes the valve SV7, the thirteenth pipe T13, the fourteenth pipe T14, the fifteenth pipe T15 and the eighteenth pipe T18, the mixing assembly 61 is further provided with a connecting piece 613. Wherein the connecting piece 613 is located between the fifteenth pipe T15 and the eighteenth pipe T18.

Specifically, as shown in fig. 4, the connecting piece 613 includes a first end, a second end, and a third end. Wherein, the first end of the connecting piece 613 is connected with the fourth port of the fifteenth pipeline T15, and the second end of the connecting piece 613 is connected with the fifth port of the eighteenth pipeline T18. Further, a third end of the connection 613 may be connected to the evacuation device 80. That is, the first end of the connection piece 613 is connected to the fifteenth pipe T15 of the bubble mixing pipe unit, and the second end communicates with the bottom of the container 10 through the eighteenth pipe T18. Thus, the connector 613 can also provide for bubble mixing.

In some embodiments, based on the liquid in the container 10 being emptied and the fifteenth tube T15 being filled with liquid, the mixing power element 62 is configured to drive the liquid in the fifteenth tube T15 to flow in a direction away from the connection element to draw gas through the container, the eighteenth tube T18, and the connection element 613 and store the gas in at least the fifteenth tube T15, i.e. it is also possible to continue to store the gas in the first passage structure due to insufficient space in the fifteenth tube T15; when it is necessary to inject the gas into the vessel 10 from the bottom of the vessel 10, the mixing power member 62 is controlled to inject the gas stored in at least the fifteenth pipe T15 of the bubble mixing pipe unit into the vessel 10 from the bottom of the vessel 10 through the fifteenth pipe T15 of the bubble mixing pipe unit, the first end of the connecting member 613, the second end of the connecting member 613, and the eighteenth pipe T18 in this order.

In some embodiments, the connector 613 may be a tee. In use, when the blending power 62 stores gas in a bubble blending line unit, such as the fifteenth line T15. At this time, only a certain air column is left in the connecting piece 613, so that the liquid in the fifteenth pipeline T15 and the eighteenth pipeline T18 are not contacted, and the liquid in the fifteenth pipeline T15 can be prevented from diffusing into the eighteenth pipeline T18.

It will be appreciated that, in addition to the structural design of the blending assembly 61 in the schematic diagram of the measurement system shown in fig. 4, other structures in the schematic diagram of the measurement system shown in fig. 4 may refer to the above contents of the measurement systems shown in fig. 1 to 3, and will not be described in detail herein.

Please refer to fig. 5 again in combination with fig. 1. Specifically, as shown in fig. 1 and 5, the blending assembly 61 in the measurement system shown in fig. 5 is mainly different from the blending assembly 61 in the measurement system shown in fig. 1 in that: the fifteenth pipeline T15 in the mixing assembly 61 is different in connection mode, namely: one end of the fifteenth pipe T15 in the mixing assembly 61 shown in fig. 1 is connected to the valve SV7 and the other end thereof is connected to the side of the isolation chamber 611, and one end of the fifteenth pipe T15 in the mixing assembly 61 shown in fig. 5 is connected to the valve SV7 and the other end thereof is connected to the bottom of the container 10. That is, the bubble mixing pipe unit and the isolation chamber 611 in the mixing assembly 61 shown in fig. 5 are directly connected to the bottom of the container 10, and the mixing power member 62 injects gas directly from the bottom of the container 10 through the bubble mixing pipe unit into the container 10, and the isolation chamber 611 and the eighteenth pipe T18 serve as buffers for liquid discharge in the container 10. And the other structures of the mixing assembly 61 shown in fig. 5 and the other structures in the measuring system are substantially identical to the structural design in fig. 1. Wherein the connection portion of the other end of the fifteenth tube T15 in the bubble mixing tube unit and the container 10 is different from the connection portion of the isolation chamber 611 and the container 10.

The air bubble mixing line unit in the mixing unit 61 shown in fig. 5 and 6 includes at least: valve SV7, including both ends; the first passage structure comprises a fourteenth pipeline T14, wherein a port of the fourteenth pipeline T14 connected with the uniform mixing power piece 62 is a first port, and a port of the fourteenth pipeline T14 connected with one end of the valve SV7 is a second port; the second passage comprises a fifteenth pipeline T15, the fifteenth pipeline T15 comprises a third port and a fourth port, the third port is connected with the other end of the valve SV7, and the fourth port is directly connected with the bottom of the container 10; wherein the valve SV7 is configured to control the connection or disconnection of the fourteenth pipe T14 and the fifteenth pipe T15. Similarly, in some embodiments, the bubble blending line unit in the blending assembly 61 shown in fig. 5 and 6 may also include the thirteenth line T13, the sixteenth line T16, and the valve SV8 as described above. That is, the connection lines between the thirteenth line T13, the sixteenth line T16, and the fourteenth line T14 may be collectively referred to as a first passage structure of the bubble mixing line unit. Namely: one end of the first passage structure is connected to the mixing power member 62, and the other end of the first passage structure is connected to one end of the valve SV 7. At this time, the valve SV7 may also be configured to control the connection or disconnection of the first passage structure and the fifteenth pipe T15. The connection relationship between the thirteenth pipeline T13, the sixteenth pipeline T16, the fourteenth pipeline T14 and the valve SV8 is substantially the same as that of the embodiment shown in fig. 1 to 4, and the detailed description thereof will be omitted herein.

In the structural design of the bubble mixing pipe unit in the mixing assembly 61 shown in fig. 5 and 6, the mixing power member 62 drives the mixing assembly 61 to suck gas through the container 10 and store the sucked gas at least in the fifteenth pipe T15, that is, may also continue to store gas in the first passage structure due to insufficient space in the fifteenth pipe T15. When it is desired to inject the gas into the vessel 10 from the bottom of the vessel 10, the mixing power member 62 drives the liquid in the bubble mixing tube unit to flow in a direction approaching the vessel 10 and injects the gas into the vessel 10 through the fifteenth tube T15 and the bottom of the vessel 10.

In other embodiments, the measurement system shown in FIG. 5 may also not include isolation chamber 611, namely: as shown in fig. 6, the bottom of the container 10 may be directly connected to the evacuation device 80 through an eighteenth line T18.

Similarly, except for the structural design of the blending assembly 61 in the measurement system shown in fig. 5 or 6, other structures in the measurement system shown in fig. 5 or 6 may refer to the above contents of the measurement systems shown in fig. 1 to 3, and will not be described in detail herein.

It should be noted that the naming of the pipes and passages in the present application is for descriptive purposes only, and in some embodiments, the naming of the pipes and passages may be interchanged according to the specific situation, for example, the fifteenth pipe T15 in the bubble mixing pipe unit may be named as the second passage, the eighteenth pipe T18 in the measurement system may be named as the third passage, etc.

Suction-back device (not shown in the figure)

In some embodiments, as shown in fig. 1-6, the sample supply device 20 or the first reagent supply device 30 or the second reagent supply device 40 in the measurement system 100 may also be used to suck back a portion of the first mixed liquor from the reaction vessel 10. Further, the sample supply device 20 or the first reagent supply device 30 or the second reagent supply device 40 may further add a part of the first mixed solution to the reaction container 10 again, so that a part of the first mixed solution is mixed with the second reagent and the third reagent to form a second mixed solution. In order to clearly illustrate the suck-back function of the sample supply device 20 or the first reagent supply device 30 or the second reagent supply device 40 in the embodiment provided by the present application, a part of elements in the sample supply device 20 or the first reagent supply device 30 or the second reagent supply device 40 that can perform the suck-back function are referred to as suck-back devices (not shown in the drawings).

In particular, the suck-back apparatus may comprise a suck-back assembly and a suck-back power member. Wherein the back suction power member is coupled to the back suction assembly such that the back suction power member can draw a portion of the first mixed liquor from the container 10 through the back suction assembly and refill the container 10 with a portion of the first mixed liquor based on the container 10 being emptied of remaining first mixed liquor. The back suction power piece can provide power for the back suction assembly to suck or discharge the first mixed liquor. The first mixed solution is formed by mixing a first reagent and a sample to be tested. It should be noted that, the process of sucking part of the first mixed liquid from the container 10 by the back suction device in the present application may also be referred to as a process of back sucking the liquid. The sucked-up portion of the first mixed liquid may also be referred to as a suck-back sample liquid.

In some embodiments, the back suction device may be part of the structure of the sample supply device 20 in the measurement system 100 shown in fig. 1-6. Namely: the back suction assembly is the sampling needle assembly 22 and the back suction power member is the sampling power member.

In some embodiments, the back suction device may be part of the structure of the first reagent supply device 30 in the measurement system 100 shown in fig. 1, 2, and 4-6. Namely: the suck-back assembly comprises at least part of a first line unit, for example a ninth line T9 and a valve SV3, or a ninth line T9, a seventh line T7, a fifth line T5 and a valve SV3. Further, the suck back assembly comprises a first pre-heater 33. Further, the suck-back power member includes a first drive member 32. In some embodiments, the structural design of the suction back device may also be referred to as a line suction back device. Accordingly, the suck-back assembly may also be referred to as a line suck-back unit.

In other embodiments, the back suction device may be part of the structure of the second reagent supply device 40 in the measurement system 100 shown in fig. 1, 2, and 4-6. Namely: the second driver 42 and part of the second tubing unit in the second reagent supply device 40 may also act as a suck-back device. Specifically, the suck-back assembly comprises at least part of a second line unit, such as a tenth line T10 and a valve SV4, or a tenth line T10, an eighth line T8, a sixth line T6 and a valve SV4. Further, the suck back assembly comprises a second pre-heater 43. Further, the suck-back power member includes a second driving member 42.

In other embodiments, the back-suction device may be part of the structure of the first reagent supply device 30/the second reagent supply device 40 in the measurement system 100 shown in FIG. 3. Specifically, the suck-back assembly comprises at least part of a third line unit, such as a seventh line T7, a valve SV3 and a valve SV2, or a seventh line T7, a sixth line T6, a fourth line T4, a third line T3, a valve SV3 and a valve SV2. Further, the suck back assembly comprises a first pre-heater 33. Further, the suck-back power member includes a first drive member 32.

In other embodiments, the measurement system 100 may further include a third drive (not shown) and a fourth tubing unit (not shown), and the suck-back device may also be the third drive and the fourth tubing unit. One end of the fourth pipeline unit is connected with the container 10, and the other end of the fourth pipeline unit is connected with the third driving piece. Upon back suction, the third driver may draw a portion of the first mixed liquor from the container 10 through the fourth line unit and refill the container 10 with a portion of the first mixed liquor based on the container 10 being emptied of remaining first mixed liquor.

Cleaning device and evacuation device 80

With continued reference to fig. 1-6, in some embodiments, the measurement system 100 may further include a cleaning device (not shown) and an evacuation device 80, each coupled to the container 10. Wherein the cleaning device is used to add cleaning fluid to the container 10. An evacuation device 80 is provided at the bottom of the container 10 to drive the liquid in the container 10 out.

In some embodiments, as shown in fig. 1-3 and 5, the evacuation device 80 is connected to the isolation chamber 611, and the evacuation device drives the liquid in the container 10 to be discharged through the eighteenth pipeline T18 and the isolation chamber 611.

In other embodiments, as shown in fig. 4, the evacuation device 80 is connected to the connection 613, and the evacuation device drives the liquid in the container 10 to be discharged through the eighteenth line T18 and the connection 613.

In other embodiments, as shown in fig. 6, the evacuation device 80 may also be connected to the bottom of the container 10 through an eighteenth line T18, and the evacuation device drives the liquid in the container 10 to be discharged through the eighteenth line T18.

Specifically, the cleaning apparatus includes a cleaning assembly including a cleaning liquid tank and a mixing power member 62 (which may also be referred to as a cleaning power member), and a corresponding pipe unit including at least a part of a first passage structure (e.g., thirteenth pipe T13, sixteenth pipe T16) connected to the cleaning assembly, an eleventh pipe T11 connected to the at least part of the first passage structure, a twelfth pipe T12 connected to one side of the container 10, and a valve SV5 connecting the eleventh pipe T11 and the twelfth pipe T12. The cleaning assembly is connected to one side of the container 10 through at least part of a first path structure (e.g., thirteenth and sixteenth pipelines T13 and T16), eleventh pipeline T11, valve SV5 and twelfth pipeline T12 in sequence, and the cleaning solution in the cleaning solution tank is injected into the container 10 under the driving of the cleaning power member to clean the inner wall of the container 10. Such a structural design not only facilitates the cleaning by injecting the cleaning fluid into the container 10 through the cleaning device after the liquid in the container 10 is discharged, but also the cleaning device and the mixing device 60 share the mixing power member 62 and at least part of the first path structure (e.g., thirteenth and sixteenth pipelines T13 and T16), thereby saving connecting pipelines, simplifying the structural layout of the apparatus, and saving space. After cleaning, the cleaning solution in the container 10 is again driven to be discharged by the emptying device 80, and the operation is repeated for a plurality of times until the container 10 is cleaned. Thus, the waste liquid residue in the container 10 can be reduced, which is advantageous for improving the accuracy of the detection result.

Further, the evacuation device 80 may be used to evacuate the liquid from the container 10. In particular, the evacuation device 80 may comprise a waste liquid tank, a liquid pump 81 connected to the waste liquid tank and the container 10, respectively, and a corresponding piping unit. The corresponding line unit comprises at least a line between the liquid pump 81 and the container 10 and a valve, wherein the line between the liquid pump 81 and the container 10 comprises a twentieth line T20 connected to the liquid pump 81 and a nineteenth line T19 connected to the container 10, and the valve comprises a valve SV6 for controlling the connection and disconnection of the evacuation device. The liquid pump 81 can provide power for discharging liquid from the container 10, so that a negative pressure is formed in the connecting pipeline, and the liquid in the container 10 is discharged. Specifically, one end of the liquid pump 81 is connected to the bottom of the container 10 through a twentieth line T20, a valve SV6, a nineteenth line T19, and an eighteenth line T18 in this order, and the other end of the liquid pump 81 is connected to a waste liquid tank.

When the liquid in the container 10 needs to be emptied, the liquid pump 81 is started, the valve SV6 is opened, and the liquid pump 81 operates to form negative pressure in the twentieth pipeline T20 and the nineteenth pipeline T19, so as to drive the liquid in the container 10 to be discharged.

In some embodiments, the evacuation device 80 may further include: isolation chamber 611 and eighteenth line T18. Thus, the eighteenth line T18 and the isolation chamber 611 connected to the bottom of the vessel 10 can serve as a buffer zone for the evacuation device 80. Further, the bottom of the isolation chamber 611 is connected to one end of the liquid pump 81 through a valve SV6, and the other end of the liquid pump 81 is open to the waste liquid tank. When the valve SV6 is opened, the waste liquid flows into the waste liquid tank through the eighteenth pipeline T18, the isolation chamber 611, the valve SV6 and the corresponding pipelines (e.g., nineteenth pipeline T19 and twentieth pipeline T20) in this order under the negative pressure provided by the liquid pump 81.

The mixing device 60 and the emptying device 80 in the measuring system share the isolation chamber 611 and the eighteenth pipeline T18 in the bubble mixing pipeline unit, so that the connecting pipeline is saved under the condition of ensuring normal functions, the structural layout of the equipment is more reasonable, and the space is saved.

In other embodiments, the evacuation device 80 further includes a filter 82. The filter 82 may be used to filter residues that may be present in the waste liquid, thereby reducing the probability of the valve SV6 being blocked and reducing the frequency of valve SV6 replacement.

Specifically, as shown in fig. 1 to 3 and 5, a filter 82 is provided between the valve SV6 and the isolation chamber 611.

Specifically, as shown in fig. 4, the filter 82 is disposed between the valve SV6 and the connection 613.

Specifically, as shown in FIG. 6, a filter 82 is disposed between the valve SV6 and the bottom of the container 10.

Since the mixing assembly 61 and the evacuation device 80 in the measurement system of the present application share the isolation chamber 611 and the eighteenth pipeline T18, a residual liquid remains on the wall of the eighteenth pipeline T18 and the wall of the isolation chamber 611 after the waste liquid is discharged. The bottom of the container 10, which is the place where the liquid mixing detection is performed, is directly connected to the eighteenth pipeline T18, so that the liquid will directly contact with the eighteenth pipeline T18 during the reaction, and if the waste liquid remains on the wall of the eighteenth pipeline T18, the gas is injected into the container 10 through the eighteenth pipeline T18 during the mixing, so that the residual waste liquid is brought into the container 10, thereby reducing the accuracy of the detection result. Therefore, in order to reduce the contact between the reacted liquid in the container 10 and the residual liquid on the wall of the eighteenth pipeline T18, the wall of the eighteenth pipeline T18 is also made of a material with good hydrophobicity, such as a teflon material, so that the liquid does not remain on the wall of the pipeline, and the influence of the residual liquid on the detection result is reduced. In other embodiments, to avoid residual waste liquid on the wall of the eighteenth pipeline T18, the length of the eighteenth pipeline T18 is designed to be as short as possible, so long as the length can meet the conditions of bubble mixing and waste liquid discharge buffering.

Further, in some embodiments, the inner walls of the twentieth and nineteenth lines T20 and T19 of the evacuation device 80 may also be made of a material that is well hydrophobic, such as a teflon material. Therefore, the waste liquid residue on the inner wall of the connecting pipeline can be further reduced, and the cleaning difficulty and the maintenance frequency are further reduced.

Principle of operation

To make the above description clearer, fig. 7a to 12 also show schematic diagrams of the method of operation embodying the working principle of the measurement system 100 according to an embodiment of the present application. It should be noted that, as described in the above embodiments, the mixing device 60, the back suction device and the third reagent supplying apparatus 50 of the present application have the structural designs of a plurality of different embodiments, and the first reagent supplying apparatus 30 and the second reagent supplying apparatus 40 have the structural designs of different embodiments, but the working principles of the measuring system 100 of a plurality of different embodiments are basically similar. Therefore, the following description will mainly take the measurement system 100 in the embodiment shown in fig. 1 as an example, and the measurement system 100 in other embodiments may refer to the following specific details of the operation method.

Step S1: the bubbles are stored and the required gases are mixed uniformly.

Referring to fig. 7a to 7f, fig. 7a to 7f are schematic diagrams illustrating the operation of step S1 in the operation method of the measurement system in the embodiment shown in fig. 1. Specifically, as shown in fig. 7a to 7f, the step S1 may include: evacuating the vessel 10 of the immersion cleaning liquid; controlling the mixing power element 62 to draw liquid from the liquid supply; a bubble mixing pipe unit communicating between the mixing power member 62 and the container 10; controlling the bubble mixing pipeline unit in the mixing assembly 61 to be full of liquid; the mixing assembly 61 is controlled to store the gas entering through the container 10, specifically, the mixing power member 62 is controlled to suck the gas through the container 10, the eighteenth pipeline T18 and the isolation chamber 611, and store the sucked gas in at least part of the bubble mixing pipeline unit, for example, in the fifteenth pipeline T15, or based on the exhaustion of the liquid in the container 10, the gas enters the isolation chamber 611, and the valves in the pipelines connected with the isolation chamber 611 are controlled to be closed, so that the gas is stored in the isolation chamber 611.

In the specific implementation, as shown in fig. 7a, since the container 10 holds the immersed cleaning solution, the cleaning solution in the container 10 needs to be emptied before the step S1 starts. At this time, the valve SV6 is opened, the draining device is started, and the other valves are closed, the immersion cleaning liquid in the container 10 is drained through the eighteenth pipeline T18, the isolation chamber 611, the filter, the valve SV6, the nineteenth pipeline T19, and the twentieth pipeline T20 by the liquid pump until the cleaning liquid in the container 10 is drained, and the valve SV6 is closed.

As shown in fig. 7b, before the reaction starts, the mixing power member 62 is controlled to fill the bubble mixing pipe unit in the mixing assembly 61 with liquid, namely: closing the valve SV7 and opening the valve SV8 to connect the sixteenth pipeline T16 with the seventeenth pipeline T17 and closing the other valves and opening the other pipelines, controlling the mixing power member 62 to move away from the opening 621 to provide negative pressure, and further sucking liquid from the liquid supply source of the bubble mixing pipeline unit by the mixing power member 62 through the sixteenth pipeline T16 and the seventeenth pipeline T17.

Then, as shown in fig. 7c, the valve SV7 is opened, and the valve SV8 is switched to connect the sixteenth pipe T16 with the thirteenth pipe T13, at this time, the mixing power element 62 is communicated with the fifteenth pipe T15 through the sixteenth pipe T16, the valve SV8, the thirteenth pipe T13, the fourteenth pipe T14 and the valve SV7 connection, and the mixing power element 62 is controlled to move in a direction approaching the opening 621 to provide positive pressure, so that the liquid sucked by the mixing power element 62 is pushed into the fifteenth pipe T15 of the bubble mixing pipe unit, and the liquid is supplied into the fifteenth pipe T15 of the bubble mixing pipe unit. Thus, each line of the bubble mixing line unit is filled with liquid, for example, the fifteenth line T15, the fourteenth line T14, and the thirteenth line T13 are all filled with liquid.

As shown in fig. 7d, valve SV8 continues to open so that the sixteenth line T16 communicates with the thirteenth line T13, while valve SV7 is open and the other valves are closed and the other lines are disconnected. At this time, the mixing power element 62 is controlled to move in a direction away from the opening 621 to provide negative pressure, and the liquid in the fifteenth pipe T15 is driven to flow in a direction away from the isolation chamber 611. Thereby, the mixing power 62 sucks the gas (e.g., air) through the container 10, the isolation chamber 611, and the bubble mixing pipe unit, so that the gas enters at least the fifteenth pipe T15 of the bubble mixing pipe unit through the container 10, the eighteenth pipe T18, and the isolation chamber 611. The control mixing power element 62 maintains the pressure in the line of the bubble mixing line unit and closes the valve SV7. At this time, the gas is stored in at least the fifteenth pipeline T15 of the bubble mixing pipeline unit (as shown in fig. 7 f). Until the sample to be measured and the reagent are mixed uniformly, the gas required for mixing the storage bubbles is completed through the step S1.

In another implementation, the gas required for bubble mixing may also be stored in the isolation chamber 611 without filling the bubble mixing pipe unit with the sucked gas, for example: fifteenth line T15. Specifically, as shown in fig. 7e, after the mixing power member 62 fills each line of the bubble mixing line unit with liquid (for example, the fifteenth line T15, the fourteenth line T14, and the thirteenth line T13 are all filled with liquid), the valve SV7 is closed. At this time, the evacuation device 80 has evacuated the liquid in the container 10 through the eighteenth pipeline T18, the isolation chamber 611 and the corresponding connection pipeline in the evacuation device 80, and thus, the gas is also stored in the isolation chamber 611. That is, after the liquid in the vessel 10 is emptied by the emptying device 80 connected to the isolation chamber 611 and the vessel 10 in sequence, the gas required for mixing the bubbles is stored in the isolation chamber 611. Therefore, in this step S1, by controlling the mixing power member 62, it is possible to suck the gas through the container 10, the eighteenth pipe T18, the isolation chamber 611, and the bubble mixing pipe unit, and store the sucked gas in at least a part of the bubble mixing pipe unit. After the liquid in the container 10 is emptied, or after the liquid in the container 10 is emptied based on the emptying device 80 connected with the isolation chamber 611 and the container 10 in sequence, the gas enters the isolation chamber, the valves in the pipes connected with the isolation chamber 611 are controlled to be closed, and the gas is stored in the isolation chamber 611.

Referring to fig. 8a to 8e, fig. 8a to 8e are schematic diagrams illustrating another operation procedure of step S1 in the operation method of the measurement system in the embodiment shown in fig. 4. Specifically, as shown in fig. 8a to 8e, the step S1 may include: evacuating the vessel 10 of the immersion cleaning liquid; controlling the mixing power element 62 to draw liquid from the liquid supply; a bubble mixing pipe unit communicating between the mixing power member 62 and the container 10; controlling the bubble mixing pipeline unit in the mixing assembly 61 to be full of liquid; the control mixing power element 62 sucks gas through the container 10, the eighteenth line T18, the connecting element 613 and the bubble mixing line unit, and stores the sucked gas in at least part of the bubble mixing line unit, for example, in the fifteenth line T15.

Since fig. 8a to 8e show the operation of step S1 of the measuring system 100 shown in fig. 4, and fig. 7a to 7d show the operation of step S1 of the measuring system 100 shown in fig. 1, as can be seen from the description of the above embodiments, the structural design of the measuring system 100 shown in fig. 4 is adjusted based on the structure of the measuring system 100 shown in fig. 1, and the main difference between them is that: the isolation chamber 611 in the mixing assembly 61 shown in fig. 1 is replaced with the connector 613 shown in fig. 4, and other structures of the mixing assembly 61 shown in fig. 4 are substantially the same as the mixing assembly 61 shown in fig. 1.

Thus, the operation of step S1 of the measuring system 100 shown in fig. 8a to 8e differs from the operation of step S1 of the measuring system 100 shown in fig. 7a to 7d mainly in that: fig. 8a to 8e show that the mixing power member 62 sucks gas through the container 10, the eighteenth line T18, the connecting member 613 and the bubble mixing line unit during the operation of step S1 of the measuring system 100, whereas fig. 7a to 7d show that the mixing power member 62 sucks gas through the container 10, the eighteenth line T18, the isolation chamber 611 and the bubble mixing line unit or enters gas through the container 10, the eighteenth line T18 and the isolation chamber 611 during the operation of step S1 of the measuring system 100. Namely: as shown in fig. 8d and 8e, after the mixing power member 62 fills each line of the bubble mixing line unit with liquid (e.g., the fifteenth line T15, the fourteenth line T14, and the thirteenth line T13 are all full of liquid), the valve SV8 is continuously opened so that the sixteenth line T16 communicates with the thirteenth line T13, while the valve SV7 is opened, and the other valves are closed and the other lines are disconnected. At this time, the mixing power element 62 is controlled to move in a direction away from the opening 621 to provide negative pressure, so that the mixing power element 62 sucks gas through the container 10, the eighteenth line T18, the connecting element 613 and the bubble mixing line unit, so that the gas enters at least the fifteenth line T15 of the bubble mixing line unit through the container 10, the eighteenth line T18 and the connecting element 613. The control mixing power element 62 maintains the pressure in the line of the bubble mixing line unit and closes the valve SV7. At this time, the gas is stored in at least the fifteenth pipeline T15 of the bubble mixing pipeline unit, as shown in fig. 8 e. Until the sample to be measured and the reagent are mixed uniformly, the gas required for mixing the storage bubbles is completed through the step S1.

While regarding the other operational procedures in step S1: evacuating the vessel 10 of the immersion cleaning liquid; controlling the mixing power element 62 to draw liquid from the liquid supply; a bubble mixing pipe unit communicating between the mixing power member 62 and the container 10; the bubble mixing pipe units in the mixing assembly 61 are controlled to be filled with liquid, and both are identical, and specific reference is made to the descriptions in fig. 7a to 7c, and details are not repeated here.

Referring to fig. 9a to 9e, fig. 9a to 9e are schematic diagrams illustrating an operation procedure of step S1 in the operation method of the measurement system in the embodiment shown in fig. 5 or 6. Specifically, as shown in fig. 9a to 9e, the step S1 may include: evacuating the vessel 10 of the immersion cleaning liquid; controlling the mixing power element 62 to draw liquid from the liquid supply; a bubble mixing pipe unit communicating between the mixing power member 62 and the container 10; controlling the bubble mixing pipeline unit in the mixing assembly 61 to be full of liquid; the control mixing power 62 sucks the gas through the container 10 and the bubble mixing tube unit, and stores the sucked gas in at least a part of the bubble mixing tube unit, for example, in at least the fifteenth tube T15.

Since fig. 9a to 9e show the operation of step S1 of the measuring system 100 shown in fig. 5, and fig. 7a to 7d show the operation of step S1 of the measuring system 100 shown in fig. 1, as can be seen from the description of the above embodiments, the structural design of the measuring system 100 shown in fig. 5 is adjusted based on the structure of the measuring system 100 shown in fig. 1, and the main difference between them is that: the fifteenth pipeline T15 in the mixing assembly 61 is different in connection mode, namely: one end of the fifteenth pipe T15 in the mixing assembly 61 shown in fig. 1 is connected to the valve SV7 and the other end thereof is connected to the side of the isolation chamber 611, whereas one end of the fifteenth pipe T15 in the mixing assembly 61 shown in fig. 5 is connected to the valve SV7 and the other end thereof is directly connected to the bottom of the container 10, and the point where the other end of the fifteenth pipe T15 is connected to the bottom of the container 10 is different from the point where the isolation chamber 611 is connected to the bottom of the container 10.

Thus, the operation of step S1 of the measurement system 100 shown in fig. 9a to 9e differs from the operation of step S1 of the measurement system 100 shown in fig. 7a to 7d mainly in that: fig. 9a to 9e show that the mixing power 62 sucks gas through the container 10 and the bubble mixing pipe unit during the operation of step S1 of the measurement system 100, and fig. 7a to 7d show that the mixing power 62 sucks gas through the container 10, the eighteenth pipe T18, the isolation chamber 611 and the bubble mixing pipe unit or enters gas through the container 10, the eighteenth pipe T18 and the isolation chamber 611 during the operation of step S1 of the measurement system 100. Namely: as shown in fig. 9d-9e, after the mixing power member 62 fills each of the lines of the bubble mixing line unit with liquid (e.g., the fifteenth line T15, the fourteenth line T14, and the thirteenth line T13 are all full of liquid), the valve SV8 is continuously opened so that the sixteenth line T16 communicates with the thirteenth line T13, while the valve SV7 is opened, and the other valves are closed and the other lines are disconnected. At this time, the mixing power element 62 is controlled to move in a direction away from the opening 621 to provide negative pressure, so that the mixing power element 62 sucks gas through the container 10 and the bubble mixing line unit, so that the gas directly enters the fifteenth line T15 of the bubble mixing line unit through the container 10. The control mixing power element 62 maintains the pressure in the line of the bubble mixing line unit and closes the valve SV7. At this time, the gas is stored in the fifteenth pipeline T15 of the bubble mixing pipeline unit, as shown in fig. 9 e. Until the sample to be measured and the reagent are mixed uniformly, the gas required for mixing the storage bubbles is completed through the step S1.

Compared with the prior art, the measuring system provided by the application has the advantages that the mixing component is connected with the bottom of the container, gas is directly stored in at least part of the mixing component through the container, an additional gas acquisition device is not needed, the overall layout of the measuring system is simplified, the overall layout of the measuring system is more compact, the space is saved, and the production cost is reduced.

Step S2: and adding the sample to be tested and the reagent.

Specifically, the step S2 may include: the sample supply means is controlled to add a sample to be measured to the container 10 and the reagent supply means is controlled to add a reagent to the container 10.

In one implementation, controlling the sample supply to add the sample to be measured to the container 10 includes: the sampling needle power piece drives the sampling needle to move along a preset track until the sampling needle moves into the sample barrel 21, and the sampling power piece sucks a sample to be detected from the sample barrel 21 through the sampling needle; subsequently, the sampling needle power member drives the sampling needle to continue along a preset orbit, the sampling needle moves out of the sample barrel 21 and into the container 10, and at this time, the sampling power member discharges the sample to be measured into the container 10 through the sampling needle. At this time, the transfer of the sample to be measured from the sample tank 21 into the container 10 is completed.

Further, controlling the reagent supply means to add reagent to the container 10 includes: the first reagent supply means is controlled to add a first reagent to the container 10, the first reagent comprising a haemolytic agent. Specifically, the first reagent is injected into the container 10 by controlling the opening and closing sequence of the valves SV1 and SV3 to control the communication state of the first line T1, the second line T2, and the first line unit while closing the other valves and opening the other lines.

In some embodiments, valve SV1 is opened while all other valves are closed, such that the first drive 32 draws the first reagent from the first reagent tank 31; subsequently, valve SV1 is closed and valve SV3 is opened, and the first actuator 32 introduces the aspirated first reagent into the container 10 via the ninth line T9.

Further, in other embodiments, the first reagent may be pre-heated by passing it through the first pre-heating member 33, and then the pre-heated first reagent may be added to the container 10. In other embodiments, the first reagent may be added to the container 10 by way of a sampling power element and a sampling needle.

Further, controlling the reagent supply means to add reagent to the container 10 includes: the second reagent supply means is controlled to add a second reagent to the container 10, the second reagent comprising a buffer. Since the second reagent supplying apparatus has a similar structure to the components of the first reagent supplying apparatus, the components have the same functions, and only the names of the components are different from those of the reagents, and therefore, the operation of adding the first reagent into the container 10 by referring to the first reagent supplying apparatus is not repeated herein. In other embodiments, the second reagent may also be added to the container 10 by way of a sampling power element and a sampling needle.

Further, controlling the reagent supply means to add reagent to the container 10 includes: a third reagent supply is controlled to add a third reagent to the container 10, the third reagent comprising a latex reagent. In particular, the transfer of the third reagent from the third reagent vessel 51 to the container 10 by the sampling needle system may be operated as described in the previous embodiment of transferring the sample to be tested from the sample vessel 21 to the container 10. In other embodiments, for example, when the measurement system samples a structural design as shown in FIG. 2, then a third reagent may be added to the container 10 by the following procedure. Namely: the valves SV4, SV9 and SV2 are controlled so that the connection lines between the second driver 42, the second pre-heater 43 and the third reagent tank 51 are in a communication state, and so that the connection line of the second reagent tank 41 and the second driver 42 is in a disconnected state (i.e., the valve SV2 is disconnected from the third line T3), the third reagent is sucked through the second driver 42 and added to the container 10.

In another implementation, controlling the reagent supply to add reagent to the container 10 may include: control the first reagent supply means to add a first reagent to the container 10 and control the third reagent supply means to add a third reagent to the container 10. Wherein the first reagent comprises a mixture of a hemolytic agent and a buffer, and the third reagent comprises a latex antibody. Specific procedures for adding the first and third reagents are as described above.

Step S3: and uniformly mixing the sample to be tested and the reagent to form a mixed solution to be tested.

Referring to fig. 10, fig. 10 is a schematic diagram illustrating an operation of step S3 of the measurement system in the embodiment shown in fig. 1. Specifically, as shown in fig. 10, in some embodiments, this step S3 may include: based on the sample and the reagent to be measured (e.g., sample diluent, buffer solution, and latex antibody, or a mixed solution of hemolytic agent and buffer solution, and latex antibody) added to the container 10, the mixing power member 62 is controlled to drive the liquid in the bubble mixing tube unit to flow in a direction close to the isolation chamber 611 and to inject the gas stored in step S1 into the container 10 through the bubble mixing tube unit, the isolation chamber 611, and the bottom of the container 10 to sufficiently mix the sample and the reagent to be measured.

Specifically, valve SV7 is opened and valve SV8 is shifted to connect the sixteenth line T16 with the thirteenth line T13 while the other valves are closed and the other lines are disconnected. At this time, the mixing power member 62 is connected to the fifteenth pipe T15 through the sixteenth pipe T16, the valve SV8, the thirteenth pipe T13, the fourteenth pipe T14, and the valve SV7, and the mixing power member 62 is controlled to move in a direction approaching the opening 621 to provide positive pressure, so that the gas stored in at least the fifteenth pipe T15 of the bubble mixing pipe unit is injected into the container 10 through the bottom of the container 10 along the fifteenth pipe T15, the isolation chamber 611, and the eighteenth pipe T18. Thus, the injected gas rises from bottom to top, and the bubbles rise under the action of the buoyancy force to locally form turbulence, so that the sample to be measured and various reagents (such as sample diluent, buffer solution and latex antibody, or mixed solution of hemolytic agent and buffer solution, and latex antibody) in the container 10 are mixed uniformly, so that the liquids therein are sufficiently mixed, and the sample to be measured and the reagents react more sufficiently, so that the mixed solution to be measured is formed.

In other embodiments, the step S3 may include: based on the sample to be measured and the first reagent being added to the container 10, the mixing power 62 is controlled to drive the liquid in the bubble mixing tube unit to flow in a direction approaching the isolation chamber 611 and to inject the gas into the container 10 through the bubble mixing tube unit, the isolation chamber 611 and the bottom of the container 10, so as to sufficiently mix the sample to be measured and the first reagent and form the first mixed liquid.

Further, the step S3 may further include: and controlling the back suction device to suck back part of the first mixed liquid. Specifically, in some embodiments, after the sample to be tested is mixed with the first reagent to form the first mixed solution, a portion of the first mixed solution may be sucked back by the suck-back device before the second reagent and the third reagent are added to the container. Further, the suck-back device may also re-add a portion of the first mixed liquor to the container 10 based on sucking back a portion of the first mixed liquor and evacuating the remaining first mixed liquor from the container 10.

The back suction device can be a sampling needle structure or a pipeline back suction device in the sample supply device.

Further, based on the addition of a part of the first mixed liquid, the second reagent and the third reagent to the container 10, the mixing power member 62 is controlled to drive the liquid in the bubble mixing tube unit to flow in a direction approaching the isolation chamber 611 and to inject the gas into the container 10 through the bubble mixing tube unit, the isolation chamber 611 and the bottom of the container 10, so as to sufficiently mix the sample to be measured and the first reagent and form the first mixed liquid. The specific operation steps are basically the same as those shown in fig. 10, and the main difference is that in the step S3, the sample to be tested and the reagent are uniformly mixed more fully through the two steps of mixing, namely: the mixing power piece 62 is controlled to inject the stored gas from the bottom of the container 10 through a fifteenth pipeline T15, an isolation chamber 611 and an eighteenth pipeline T18 in sequence, and the sample to be tested and the first reagent are fully mixed; then, part of the first mixed solution is sucked back through the suck-back device, and the mixing power piece 62 is controlled again to inject the stored gas from the bottom of the container 10 through the fifteenth pipeline T15, the isolation chamber 611 and the eighteenth pipeline T18 in sequence, so as to mix the sucked back part of the first mixed solution with the second reagent and the third reagent again. Therefore, through twice mixing and the back suction of the part of the first mixed liquid, the sample to be detected is more fully mixed with various reagents in the detection process, and the use amounts of the second reagent and the third reagent in the detection process are effectively reduced. Referring to fig. 11, fig. 11 is a schematic diagram illustrating another operation procedure of step S3 of the measurement system in the embodiment shown in fig. 1. Specifically, as shown in fig. 11, in some embodiments, this step S3 may include: based on the sample to be measured and the reagent being added to the container 10, the mixing power member 62 is controlled to drive the liquid in the bubble mixing pipe unit into the isolation chamber 611 to squeeze the gas stored in the isolation chamber 611, so that the gas is injected into the container 10 through the isolation chamber 611, the eighteenth pipe, and the bottom of the container 10 to sufficiently mix the sample to be measured and the reagent.

Specifically, valve SV7 is opened and valve SV8 is shifted to connect the sixteenth line T16 with the thirteenth line T13 while the other valves are closed and the other lines are disconnected. At this time, the mixing power member 62 is connected to the fifteenth pipe T15 through the sixteenth pipe T16, the valve SV8, the thirteenth pipe T13, the fourteenth pipe T14 and the valve SV7, and the mixing power member 62 is controlled to move in a direction approaching the opening 621 to provide positive pressure, and then the liquid in the bubble mixing pipe unit is driven to enter the isolation chamber 611 to squeeze the gas stored in the isolation chamber 611, so that the gas in the isolation chamber 611 is compressed. At this time, a part of the gas in the isolation chamber 611 rises along the eighteenth line T18, that is, the gas stored in the isolation chamber 611 is injected into the container 10 through the bottom of the container 10 along the eighteenth line T18. Thus, the injected gas rises from bottom to top, and the bubbles rise under the action of the buoyancy force to locally form turbulence, so as to mix the sample to be tested and various reagents in the container 10, so that the liquids therein are fully mixed, and meanwhile, the sample to be tested and the reagents react more fully, thereby forming the mixed liquid to be tested.

It can be understood that in the step S3, the sample to be measured and the reagent may be uniformly mixed more sufficiently through two mixing operations, and the amounts of the second reagent and the third reagent may be effectively reduced. The specific steps are substantially similar to the twice-mixing operation of step S3 shown in fig. 11, and reference is made to the above.

Referring to fig. 12, fig. 12 is a schematic diagram showing an operation procedure of step S3 in the operation method of the measurement system in the embodiment shown in fig. 4. Specifically, as shown in fig. 12, this step S3 may include: based on the sample to be measured and the reagent being added to the container 10, the mixing power member 62 is controlled to drive the liquid in the bubble mixing pipe unit to flow in a direction close to the connecting member 613 and to inject the gas into the container 10 through the bubble mixing pipe unit, the connecting member 613, the eighteenth pipe T18 and the bottom of the container 10, so as to sufficiently mix the sample to be measured and the reagent.

Specifically, valve SV7 is opened and valve SV8 is shifted to connect the sixteenth line T16 with the thirteenth line T13 while the other valves are closed and the other lines are disconnected. At this time, the mixing power member 62 is connected to the fifteenth pipe T15 through the sixteenth pipe T16, the valve SV8, the thirteenth pipe T13, the fourteenth pipe T14, and the valve SV7, and the mixing power member 62 is controlled to move in a direction approaching the opening 621 to provide positive pressure, so that the gas stored in the fifteenth pipe T15 of the bubble mixing pipe unit is injected into the container 10 through the bottom of the container 10 along the fifteenth pipe T15, the connection member 613, and the eighteenth pipe T18. Thus, the injected gas rises from bottom to top, and the bubbles rise under the action of the buoyancy force to locally form turbulence, so as to mix the sample to be tested and various reagents in the container 10, so that the liquids therein are fully mixed, and meanwhile, the sample to be tested and the reagents react more fully, thereby forming the mixed liquid to be tested. Similarly, in the step S3, the sample to be measured and the reagent may be uniformly mixed more sufficiently through two mixing operations, and the amounts of the second reagent and the third reagent may be effectively reduced. The specific steps are substantially similar to the two-time mixing operation of step S3 shown in fig. 10, and reference is made to the above.

Referring to fig. 13, fig. 13 is a schematic diagram showing an operation procedure of step S3 in the operation method of the measurement system in the embodiment shown in fig. 5. Specifically, as shown in fig. 13, this step S3 may include: based on the sample to be measured and the reagent being added to the container 10, the mixing power member 62 is controlled to drive the liquid in the bubble mixing tube unit to flow in a direction approaching the container 10 and to inject the gas into the container 10 through the fifteenth tube T15 in the bubble mixing tube unit and the bottom of the container 10 to sufficiently mix the sample to be measured and the reagent.

Specifically, valve SV7 is opened and valve SV8 is shifted to connect the sixteenth line T16 with the thirteenth line T13 while the other valves are closed and the other lines are disconnected. At this time, the mixing power member 62 is connected to the fifteenth pipe T15 through the sixteenth pipe T16, the valve SV8, the thirteenth pipe T13, the fourteenth pipe T14, and the valve SV7, and the mixing power member 62 is controlled to move in a direction approaching the opening 621 to provide positive pressure, so that the gas stored in the fifteenth pipe T15 of the bubble mixing pipe unit is injected into the container 10 along the fifteenth pipe T15 and through the bottom of the container 10. Thus, the injected gas rises from bottom to top, and the bubbles rise under the action of the buoyancy force to locally form turbulence, so as to mix the sample to be tested and various reagents in the container 10, so that the liquids therein are fully mixed, and meanwhile, the sample to be tested and the reagents react more fully, thereby forming the mixed liquid to be tested.

Similarly, in the step S3, the sample to be measured and the reagent may be uniformly mixed more sufficiently through two mixing operations, and the amounts of the second reagent and the third reagent may be effectively reduced. The specific steps are substantially similar to the two-time mixing operation of step S3 shown in fig. 10, and reference is made to the above.

Step S4: and detecting the mixed liquid to be detected to obtain a detection result of the sample to be detected.

And (3) after the sample to be tested and the reagent are fully and uniformly mixed, forming a mixed solution to be tested, and then detecting the mixed solution to be tested by a projection and/or scattering turbidimetry method. And obtaining the content of the specific protein in the sample to be detected through certain calculation. Or detecting the mixed liquid to be detected by an impedance method, a colorimetry and/or a laser scattering method, and obtaining the conventional blood content in the sample to be detected by certain calculation. Thus, the detection operation of the sample to be detected is completed.

Step S5: the container 10 is cleaned and the initial state is restored.

Referring to fig. 14, fig. 14 shows the operation of step S5 in the operation method of the measurement system in the embodiment shown in fig. 1. As shown in fig. 14, when the detection of the mixed liquid to be detected is completed, the evacuation device 80 is activated, and the valve SV6 is opened to evacuate the liquid in the container 10. When the mixture to be tested is empty, valve SV6 is closed. At the same time, the cleaning device is started, the valve SV5 is opened, the cleaning liquid is injected into the container 10, and then the valve SV5 is closed, so that the container 10 is cleaned. After the cleaning is completed, the cleaning solution in the container 10 is continuously emptied by the emptying device 80. The above steps of draining the liquid, injecting the cleaning liquid, and draining the liquid are repeated so many times until the container 10 is cleaned.

And, after the cleaning liquid is injected into the container 10 through the cleaning device for the last time, the cleaning liquid is left in the container 10 without being emptied for soaking the container 10. And when the next sample to be detected is detected, discharging the soaking cleaning liquid. Thus, the test of the sample to be tested is completed once and the measurement system 100 is restored to the initial state to prepare for the test of the next sample to be tested.

It can be understood that when the measurement system adopts other structural designs, the operation process of the measurement system is substantially the same as that of the step S5 shown in fig. 14 when the measurement system performs the step S5, and the detailed description thereof will not be repeated.

Compared with the prior art, the measuring system provided by the application has the advantages that the mixing component 61 is connected with the bottom of the container 10, gas is directly stored in at least part of the mixing component through the container, an additional gas acquisition device is not needed, the overall layout of the measuring system is simplified, the overall layout of the measuring system is more compact, the space is saved, and the production cost is reduced. By further controlling the mixing power part 62, gas is injected into the container 10 from the bottom of the container 10 through the mixing assembly 61, so that the gas rises from bottom to top, bubbles rise under the action of buoyancy, turbulence is formed locally, and then the sample to be measured and the reagent are fully mixed, so that the sample to be measured and the reagent fully react, and the measurement accuracy is effectively improved. Meanwhile, the gas in the application is driven by the mixing power piece 62, so that the mixing assembly sucks the gas through the container and stores the sucked gas in the mixing assembly, and the gas is directly injected from the bottom of the container 10 through the bubble mixing pipeline unit in the mixing assembly 61 under the driving of the mixing power piece 62, so that the sample to be tested and the reagent always react in the container 10, thereby avoiding the problem that the sample to be tested and the reagent react to generate insoluble compound to cause the compound to be easily adhered in the inner wall of the sucking and spitting mixing pipeline, reducing the pipeline blockage or carrying pollution risk caused by the mixing process, and reducing the cleaning difficulty.

Further, in the measurement system of the present application, when the sample to be measured and the first reagent are mixed, the mixing assembly 61 is connected to the bottom of the container 10, so that the mixing assembly 61 can store the gas in at least part of the mixing assembly 61 through the container 10, and further control the mixing power member 62 to inject the gas into the container 10 through the bottom of the container 10 through the mixing assembly 61, so that the sample to be measured is fully mixed with the first reagent to form the first mixed solution. Then the second reagent and the third reagent are added into the container 10, and gas is injected into the container 10 through the bottom of the container 10 by controlling the mixing power piece 62 through the mixing component 61, so that the first mixed solution is fully mixed with the second reagent and the third reagent to form the mixed solution to be tested. Thus, the measuring system of the present application injects the gas into the container 10 from the bottom of the container 10 through the mixing assembly 61 for a plurality of times, so that the gas rises from bottom to top, and is beneficial to the rising of bubbles under the action of buoyancy, and turbulence is formed locally, so that the sample to be measured is fully mixed with the first reagent, the second reagent and the third reagent, so that the sample to be measured reacts more fully with the reagents, and the measuring accuracy is further improved.

Referring to fig. 15, fig. 15 is a schematic diagram illustrating a sample analyzer according to an embodiment of the application. As shown in fig. 15, a sample analyzer 200 of the present disclosure includes: a housing 210; a blood routine function module 220 disposed within the housing 210; a specific protein detection module 230 disposed within the housing; and the measurement system 100 described above for measurement analysis of a particular protein. The sample analyzer 200 of the present embodiment has the same advantages as the measurement system 100 provided in the present embodiment, and will not be described herein.

The foregoing description is only a partial embodiment of the present application, and is not intended to limit the scope of the present application, and all equivalent devices or equivalent processes using the descriptions and the drawings of the present application or directly or indirectly applied to other related technical fields are included in the scope of the present application.

Claims

1. A measurement system, comprising:

a container;

Sample supply means configured to add a sample to be measured to the container;

A reagent supply configured to supply a reagent and add the reagent to the container; and

Mixing device, connect in the bottom of container, and mixing device includes:

The mixing component is connected with the bottom of the container; and

The mixing power piece is connected with the mixing assembly;

Wherein the mixing assembly is configured to store gas entering through the container, and the mixing power element is configured to inject the gas into the container through the bottom of the container by the mixing assembly to mix the reagent and the sample to be measured.

2. The measurement system of claim 1, wherein the mixing assembly is at least partially filled with liquid before the mixing assembly is used to store gas entering through the container.

3. The measurement system of claim 2, wherein, based on the blending assembly being in communication with the blending power, the blending power is configured to drive liquid in the blending assembly to flow away from the container, and the blending assembly is configured to store the gas entering through the container; and

Based on the sample to be measured and the reagent being added to the container, the mixing power piece is further configured to drive the liquid in the rest part of the mixing assembly to flow towards a direction close to the container and inject the gas into the container through the bottom of the container so as to mix the sample to be measured and the reagent.

4. A measurement system according to claim 3, wherein the mixing assembly comprises at least a bubble mixing line unit comprising at least:

The valve comprises two ends;

The first passage structure comprises a first port and a second port, the first port is connected with the uniform mixing power piece, and the second port is connected with one end of the valve;

The second passage comprises a third port and a fourth port, and the third port is connected with the other end of the valve; and

A third passageway comprising a fifth port and a sixth port, the fifth port being connected to the fourth port and the sixth port being connected to the bottom of the container;

Wherein the valve is configured to control the communication or disconnection of the first passage structure with the second passage.

5. The measurement system of claim 4, wherein the blending assembly further comprises: an isolation chamber located between the second passage and the third passage;

The isolation chamber is a closed container and comprises a top, a bottom arranged opposite to the top and a side edge connected with the top and the bottom respectively, wherein the top is connected with the fifth port of the third passage, and the side edge is connected with the fourth port of the second passage;

Based on the second passageway being filled with the liquid, the mixing power element is configured to drive the liquid in the second passageway to flow in a direction away from the isolation chamber to draw gas through the container, the third passageway, and the isolation chamber and store the gas in a portion of the bubble mixing piping unit; and

Based on the sample to be measured and the reagent being added to the container, the mixing power piece is further configured to drive the liquid in the remaining part of the bubble mixing pipeline unit to flow towards a direction close to the isolation chamber and inject the gas into the container through the isolation chamber, the third passage and the bottom of the container so as to sufficiently mix the sample to be measured and the reagent.

6. The measurement system of claim 4, wherein the blending assembly further comprises an isolation chamber located between the second passageway and the third passageway;

The isolation chamber is a closed container and comprises a top, a bottom arranged opposite to the top and side edges connected with the top and the bottom respectively, the top is connected with the fifth port of the third passage, and the side edges are connected with the fourth port of the second passage; the isolation chamber is configured to store the gas entering through the container;

Based on the second path being filled with the liquid and the sample to be measured and the reagent being added to the container, the mixing power element is configured to drive the liquid in the second path into the isolation chamber to squeeze the gas stored in the isolation chamber, so that the gas is injected into the container through the isolation chamber, the third path and the bottom of the container to sufficiently mix the sample to be measured and the reagent.

7. The measurement system of claim 4, wherein the blending assembly further comprises a connector between the second passageway and the third passageway;

The connecting piece comprises a first end, a second end and a third end, wherein the first end is connected with the fourth port of the second passage, and the second end is connected with the fifth port of the third passage;

Based on the second passageway being filled with the liquid, the mixing power element is configured to drive the liquid in the second passageway to flow in a direction away from the connection element to draw gas through the container, the third passageway, and the connection element and store the gas in a portion of the bubble mixing piping unit; and

Based on the sample to be measured and the reagent being added to the container, the mixing power member is further configured to drive the liquid in the remaining part of the bubble mixing pipeline unit to flow in a direction close to the connecting member and to inject the gas into the container through the connecting member, the third passage and the container bottom so as to sufficiently mix the sample to be measured and the reagent.

8. A measurement system according to claim 3, wherein the mixing assembly comprises at least a bubble mixing line unit comprising at least:

The valve comprises two ends;

The first passage structure comprises a first port and a second port, the first port is connected with the uniform mixing power piece, and the second port is connected with one end of the valve; and

The second passage comprises a third port and a fourth port, the third port is connected with the other end of the valve, and the fourth port is connected with the bottom of the container;

9. The measurement system of claim 8, wherein, based on the bubble blending line unit being filled with the liquid, the blending power is configured to drive the liquid in the bubble blending line unit to flow in a direction away from the container to draw gas through the container and store the gas in a portion of the bubble blending line unit; and

Based on the sample to be measured and the reagent being added to the container, the mixing power piece is further configured to drive the liquid in the remaining part of the bubble mixing pipeline unit to flow towards a direction close to the container and to inject the gas into the container through the second passage and the bottom of the container so as to sufficiently mix the sample to be measured and the reagent.

10. The measurement system of claim 4 or 8, further comprising a cleaning device, wherein the cleaning device shares the blending power component and at least a portion of the first pathway structure with the blending device.

11. The measurement system of claim 1, wherein the reagent supply device comprises:

A first reagent supply configured to provide a sample diluent and add the sample diluent to the container;

A second reagent supply configured to provide a buffer and add the buffer to the container; and

A third reagent supply configured to provide a latex antibody and add the latex antibody to the container;

Wherein, based on the sample diluent and the sample to be measured being added to the container, the mixing power piece is configured to inject the gas into the container through the bottom of the container by the mixing component so as to mix the sample diluent and the sample to be measured.

12. The measurement system of claim 1, wherein the reagent supply device comprises:

A first reagent supply device configured to supply a mixed solution of a hemolytic agent and a buffer solution, and to add the mixed solution to the container; and

Wherein, based on the mixed solution of the hemolytic agent and the buffer solution, the sample to be tested and the latex antibody are added into the container, the mixing power piece is configured to inject the gas into the container through the bottom of the container by the mixing component so as to mix the hemolytic agent, the buffer solution, the sample to be tested and the latex antibody.

13. The measurement system of claim 1, wherein the blending power element comprises one of a syringe, a fixed displacement pump, a syringe pump, or a positive-negative pressure source.

14. A sample analyzer, comprising:

a housing;

A blood routine functional module disposed within the housing;

A specific protein detection module disposed within the housing and comprising a measurement system according to any one of claims 1-13 for use in a measurement analysis of a specific protein.