CN220812450U - Smooth muscle experiment system capable of accurately controlling preload - Google Patents
Smooth muscle experiment system capable of accurately controlling preload Download PDFInfo
- Publication number
- CN220812450U CN220812450U CN202322122033.0U CN202322122033U CN220812450U CN 220812450 U CN220812450 U CN 220812450U CN 202322122033 U CN202322122033 U CN 202322122033U CN 220812450 U CN220812450 U CN 220812450U
- Authority
- CN
- China
- Prior art keywords
- smooth muscle
- tube
- experimental
- pipe
- fixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002474 experimental method Methods 0.000 title claims abstract description 50
- 210000002460 smooth muscle Anatomy 0.000 title claims abstract description 36
- 230000036316 preload Effects 0.000 title claims abstract description 18
- 238000009423 ventilation Methods 0.000 claims abstract description 14
- 230000026683 transduction Effects 0.000 claims abstract description 7
- 238000010361 transduction Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 36
- 238000003860 storage Methods 0.000 claims description 13
- 239000002699 waste material Substances 0.000 claims description 11
- 238000005485 electric heating Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 2
- 230000000968 intestinal effect Effects 0.000 description 17
- 239000003814 drug Substances 0.000 description 5
- 238000011010 flushing procedure Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 210000001198 duodenum Anatomy 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 230000000630 rising effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- UCTWMZQNUQWSLP-VIFPVBQESA-N (R)-adrenaline Chemical compound CNC[C@H](O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-VIFPVBQESA-N 0.000 description 1
- 229930182837 (R)-adrenaline Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000002183 duodenal effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960005139 epinephrine Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001020 rhythmical effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 230000016160 smooth muscle contraction Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Landscapes
- Devices For Use In Laboratory Experiments (AREA)
Abstract
The utility model discloses a smooth muscle experiment system capable of accurately controlling preload, which relates to the technical field of biological experiments and comprises a shell and an internal operation device, wherein the internal operation device comprises a ventilation part, an experiment tube, a tension transduction part, an automatic lifting rod and a control panel, the ventilation part supplies gas for the experiment tube, the tension transduction part comprises a tension transducer and a fixing piece, the tension transducer is fixed on the automatic lifting rod through the fixing piece, and the connection between the automatic lifting rod and a motor is controlled by the control panel. Compared with the traditional smooth muscle experimental system, the utility model can rapidly and accurately control the preload, so that the control reaches millimeter level, and the experimental success rate is improved.
Description
Technical Field
The utility model relates to the technical field of biological experiments, in particular to a smooth muscle experiment system capable of accurately controlling preload.
Background
In medical school, a worker of the family or a researcher can conduct animal observation experiments, and understand the physiological characteristics of the automatic rhythmicity, the extensibility and the sensitivity of the smooth muscle of the digestive tract to chemical substances, temperature changes, stretching stimulus and the like by observing how the physical and chemical factors or medicines have influence on the contractility of the smooth muscle of the isolated small intestine.
The constant temperature smooth muscle experiment system is an instrument and equipment of a biological function experiment system, and is mainly used for researching physiological experiments of smooth muscle or isolated intestinal canal and the like, and can intuitively observe different reactions of the smooth muscle by adjusting factors such as medicines, temperature, PH and the like under the condition of maintaining the temperature of experimental environment (experimental liquid) and guaranteeing the physiological activity of isolated smooth muscle.
In experiments, tension transducers are typically used to test the change in smooth muscle contractility. When the experiment, need ligature to intestinal section both ends, one end is in experimental tube bottom, and one end is connected with tension transducer, and the shrink that needs adjustment preload made intestinal section after the connection can be recorded on tension transducer as far as possible, but the constant temperature smooth muscle system on the market all adopts manual regulation's mode to control preload at present, can't carry out millimeter level control, and the in-process is easy because of the overload makes intestinal section fracture in the experiment to lead to experimental failure.
Disclosure of utility model
The utility model aims to overcome the defects of the prior art, and provides a smooth muscle experiment system capable of accurately controlling preload, so that the success rate of the experiment is improved.
The aim of the utility model is realized by the following technical scheme:
A smooth muscle experiment system capable of accurately controlling preload comprises a shell and an internal operation device, wherein the internal operation device comprises a ventilation component, an experiment tube, a tension transduction component, an automatic lifting rod and a control panel. The tension transducer comprises a tension transducer and a fixing piece, the tension transducer is fixed on an automatic lifting rod through the fixing piece, and the automatic lifting rod is connected with a motor and controlled by a control panel.
Further, an air inlet pipe is connected to the outlet of the ventilation component, and the tail end of the air inlet pipe is connected with an air inlet needle which is bent upwards so as to prevent overlarge air bubbles.
Further, set up fixed clamping piece on the experimental tube, fixed clamping piece upper end bending forms the couple and can dismantle the connection on the experimental tube, and fixed clamping piece lower extreme is equipped with the sample fixed column, and intestines section both ends are fixed on sample fixed column and tension transducer through the fine rule, the intake pipe is fixed on fixed clamping piece by last buckle and the lower buckle on the fixed clamping piece.
In the experiment, the smooth muscle change is usually observed by adding medicine for many times, and when the liquid is changed, the pipeline is generally washed for 3 times, the steps are complicated, the liquid transferring amount can not be automatically controlled each time, and the liquid transferring amount is manually controlled by naked eyes.
In order to solve the problem of complicated pipetting operations, a preheating pipe is further arranged in the smooth muscle experiment system, an opening at the bottom of the preheating pipe is communicated with the bottom of the experiment pipe through a liquid exchange pipe, the liquid exchange pipe is connected with a water pump, and the water pump is used for controlling the water exchange amount through a control panel.
Furthermore, a liquid storage pipe is arranged in the smooth muscle experiment system and is detachably connected in the internal operation device, and when the liquid in the preheating pipe is insufficient, the liquid storage pipe is taken out to supplement the liquid for the preheating pipe.
In order to better provide a constant temperature environment, further, a constant temperature component is arranged in the smooth muscle experiment system, and the constant temperature component can be a water bath constant temperature pool or an electric heating plate.
Further, a waste liquid box is arranged in the smooth muscle experiment system and embedded on the shell, and the bottom of the experiment tube is communicated with the waste liquid box through a liquid discharge tube and a water pump.
Preferably, the fixing piece consists of a fixing body and two knobs, wherein an opening hole below the fixing body is inserted into the top of the automatic lifting rod and is fixed by the second knob; the side surface of the fixing body is provided with an opening, the tension transducer is embedded in the opening and is fixed by the first knob.
The beneficial effects of the utility model are as follows:
(1) Compared with the existing constant-temperature smooth muscle test system, the utility model controls the motor through the control panel, controls the automatic lifting rod through the motor, and then carries out rapid and accurate preload control according to different experimental requirements, wherein the control reaches millimeter level, and the precision is 1.5mm, thereby improving the success rate of experiments.
(2) According to the utility model, the preheating pipe is communicated with the experimental pipe, and the automatic liquid transferring and discharging of the experimental pipe is realized through the control of the water pump and the control panel, so that the complexity of experimental steps is reduced, and the experimental pipe can be automatically and circularly washed according to experimental requirements, so that the operation is simpler and more convenient.
Drawings
FIG. 1 is a schematic diagram of a constant temperature smooth muscle experiment system in front;
FIG. 2 is a schematic diagram of the back of a constant temperature smooth muscle experiment system;
Fig. 3 is a schematic view of an air inlet part at a 45 degree angle;
FIG. 4 is a schematic view of the back of an intake plate;
FIG. 5 is a schematic illustration of intestinal segment fixation;
FIG. 6 is a schematic diagram of a control panel;
In the figure, the device comprises a 1-ventilation component, a 2-preheating pipe, a 3-experiment pipe, a 4-liquid storage pipe, a 5-waste liquid box, a 6-constant temperature water bath, a 61-water bath, a 62-drainage switch, a 63-drainage port, a 7-tension energy conversion component, a 71-tension energy converter, a 72-fixing piece, an 8-automatic lifting rod, a 9-air inlet component, a 91-air inlet pipe, a 92-fixing clamping piece, a 93-specimen fixing column, a 94-upper clamping piece, a 95-lower clamping piece, a 96-air inlet needle, a 10-control panel, 11-silk threads, a 12-specimen, a 1-first knob, a 2-second knob and a 3-fixing body.
Detailed Description
The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by a person skilled in the art without any inventive effort, are intended to be within the scope of the present utility model, based on the embodiments of the present utility model.
Referring to fig. 1 and 2, the present utility model provides a technical solution:
Smooth muscle experimental system of accurate control preload of ability, including shell and internal operating device, internal operating device includes ventilation unit 1, experimental tube 3, tension transduction unit 7, automatic rising pole 8 and control panel 10, ventilation unit 1 provides gas for experimental tube 3, tension transduction unit 7 includes tension transducer 71 and mounting 72, and tension transducer 71 is fixed on automatic rising pole 8 through mounting 72, and automatic rising pole 8 is connected by control panel 10 with the motor and is controlled.
In this embodiment, for the control of preload, the electric control lifting rod is adopted to adjust the preload of millimeter level, the height of the support can be quickly raised by short pressing and 1.5mm height can be quickly raised by long pressing, and the preload can be quickly and accurately controlled by different experimental requirements.
The ventilation unit 1 is connected to an air intake pipe 91, and an air intake needle 96 bent upward is connected to the end of the air intake pipe 91, and the air outlet speed of the air intake needle 96 is controlled by controlling the air inlet speed of the ventilation unit 1.
As a specific structure, as shown in fig. 3, a fixing clip 92 is detachably connected to the experimental tube 3, the upper end of the fixing clip 92 is bent to form a hook to be fixed on the experimental tube 3, a specimen fixing column 93 is arranged at the lower end of the fixing clip 92, two ends of a specimen 12 are fixed on the specimen fixing column 93 and the tension transducer 71 through a silk thread 11, and a specimen fixing schematic diagram is shown in fig. 5; the air inlet pipe 91 is fixed to the fixing clip 92 by an upper clip 94 and a lower clip 95 on the fixing clip 92, as shown in fig. 4.
A preheating pipe 2 and a liquid storage pipe 4 are arranged on two sides of the experimental pipe 3, an opening at the bottom of the experimental pipe 3 is communicated with the bottom of the preheating pipe 2 through a liquid exchange pipe, the liquid exchange pipe is connected with a water pump, and the water pump controls the water exchange amount through a control panel 10; the liquid storage pipe 4 is detachably arranged in the internal operation device, and when the liquid in the preheating pipe 2 is insufficient, the liquid storage pipe 4 is taken down to supplement the liquid for the preheating pipe 2.
In the embodiment, the experimental tube has different capacities (10 ml/20ml/40 ml), in the experiment, the experimental tube can be replaced according to different requirements, the capacity on the panel is selected after the replacement, and the automatic pipetting with different capacities can be completed by clicking the pipetting button; in the experiment, the liquid in the experiment tube needs to be replaced generally, so that other liquid medicine or impurities in the liquid can be washed, the system in the embodiment has an automatic washing function, the liquid in the experiment tube can be automatically discharged and added and circulated for 3 times, and the aim of washing is achieved, and the control panel is shown in fig. 6.
In order to provide the constant temperature condition, experiments can be performed in a constant temperature environment, or constant temperature components such as an electric heating plate, a water bath constant temperature pool and the like are arranged in a smooth muscle experiment system, the water bath constant temperature pool is adopted in the embodiment, the experiment tube 3, the preheating tube 2 and the liquid storage tube 4 are all arranged in the water bath pool 61, a constant temperature heating component is arranged in the water bath pool 61, and a water outlet 63 and a water draining switch 62 are arranged on the water bath pool 61. As another example, the constant temperature component 6 of the application is an electric heating plate, and the experiment tube 3, the preheating tube 2 and the liquid storage tube 4 are all arranged on the electric heating plate.
As a specific structure, a waste liquid box 5 is arranged on the smooth muscle experiment system, the waste liquid box 5 is embedded on the shell, and the bottom of the experiment tube 3 is communicated with the waste liquid box 5 through a liquid discharge tube and a water pump.
The fixing member may be a bolt, a nut, a strap, a movable ring, or other components or fixed by welding, and in this embodiment, the fixing member 72 is preferably composed of a fixing body a3 and two knobs, and the opening below the fixing body a3 is inserted into the top of the automatic lifting rod 8 and fixed by the second knob a 2; the fixed body a3 is opened at the side surface, and the tension transducer 71 is fitted in the opening and fixed by the first knob a 1.
The motor and lifting rod used in the embodiment come from a double-guide-rail open type screw rod module FSK80 of the Chengdu reputation science and technology limited company, the control precision reaches millimeter level, and the precision is 1.5mm.
The application method of the utility model comprises the following steps:
Taking the duodenum of a rabbit as an example:
1. Preparation before experiments
① External circulation preheating: before the experiment, the constant temperature pond is filled with about 2L of purified water, the temperature of the constant temperature pond is adjusted to 37 ℃ on a control panel, a heating key is pressed, and the circulating water temperature of the external circulation is preheated to 37 ℃.
② Desk top fluid (a physiological solution that keeps the isolated intestinal muscle active) pre-warms: the bench type liquid is poured into a preheating pipe and a liquid storage pipe for preheating.
③ Instrument connection: the tension transducer is fixed on the automatic lifting rod for fixing the subsequent intestinal section.
④ Selection of experimental tubes: according to the intestinal canal of the experimental animal, the size of the corresponding experimental canal is selected, the duodenal experiment of the rabbit is performed, 20mL of experimental canal is selected, and 20mL of experimental canal is selected on a control panel for correspondence.
⑤ Liquid ventilation: and fixing the fixed clamping piece in the experimental tube, adjusting the air inflow of the ventilation component, and observing bubbles in the experimental tube, wherein the speed of about 1-2/s is most suitable.
2. In experiments
① About 1 cm of duodenum of a rabbit is taken, and threads are fixed at two ends of the section of the duodenum, as shown in figure 3.
② Preload adjustment: the traction preload is adjusted, so that the preload ensures that the intestinal section can shrink freely, and the tension transducer can accurately record the shrinkage condition of the intestinal section.
③ Intestinal canal irrigation: when the intestinal segment is first put in, blood stains are more, and intestinal canal flushing is needed (the size of the experimental tube is 20mL before the experiment, so the liquid amount is 20mL when flushing). The flushing function can be started by long-time pressing of the pipetting and drainage keys for two seconds, at the moment, the bench liquid of the experimental tube can automatically drain to the waste liquid box, and then the bench liquid of the preheating tube enters the experimental tube, so that the flushing of the intestinal tube is completed for 3 times.
④ Experimental records: the spontaneous rhythmic contractions of smooth muscle were observed and a normal contractile curve was recorded.
⑤ And (3) adding medicines: after addition of epinephrine, smooth muscle contraction is observed
⑥ Intestinal canal irrigation: and starting a flushing function, and continuing the experiment after replacing the experiment liquid.
3. End of experiment
① Taking out the intestinal canal: cutting the silk thread to take out the intestinal segment
② Liquid discharge: discharge bench type liquid in experimental tube to waste liquid box
③ External circulation liquid discharge: the water bath draining switch is turned on, and the purified water of the external circulating water bath is drained from the water bath draining port.
The foregoing is merely a preferred embodiment of the utility model, and it is to be understood that the utility model is not limited to the form disclosed herein but is not to be construed as excluding other embodiments, but is capable of numerous other combinations, modifications and environments and is capable of modifications within the scope of the inventive concept, either as taught or as a matter of routine skill or knowledge in the relevant art. And that modifications and variations which do not depart from the spirit and scope of the utility model are intended to be within the scope of the appended claims.
Claims (9)
1. Smooth muscle experimental system that can accurate control preload, including shell and inside operating means, its characterized in that: the internal operation device comprises a ventilation component (1), an experiment tube (3), a tension transduction component (7), an automatic lifting rod (8) and a control panel (10);
The ventilation component (1) provides gas for the experimental tube (3), the tension transduction component (7) comprises a tension transducer (71) and a fixing piece (72), the tension transducer (71) is fixed on an automatic lifting rod (8) through the fixing piece (72), and the automatic lifting rod (8) is connected with a motor and controlled by a control panel (10).
2. The smooth muscle testing system of claim 1, wherein: the air inlet pipe (91) is connected with the ventilation component (1), and the tail end of the air inlet pipe (91) is connected with an air inlet needle (96) which is bent upwards.
3. The smooth muscle testing system of claim 2, wherein: still including fixed clamping piece (92), fixed clamping piece (92) upper end bending forms the couple and can dismantle and connect on experimental tube (3), fixed clamping piece (92) lower extreme is equipped with sample fixed column (93), and sample (12) both ends are fixed on sample fixed column (93) and tension transducer (71) through silk thread (11), intake pipe (91) are fixed on fixed clamping piece (92) by last buckle (94) and lower buckle (95) on fixed clamping piece (92).
4. The smooth muscle testing system of claim 1, wherein: the device comprises a preheating pipe (2), wherein a hole at the bottom of the preheating pipe (2) is communicated with the bottom of an experimental pipe (3) through a liquid exchange pipe, the liquid exchange pipe is connected with a water pump, and the water pump is controlled by a control panel (10) to exchange water.
5. The smooth muscle testing system of claim 4, wherein: the device also comprises a liquid storage pipe (4), and the liquid storage pipe (4) is detachably connected in the internal operation device.
6. The smooth muscle testing system of claim 5, wherein: the water bath device comprises a constant temperature component (6), wherein the constant temperature component (6) is a water bath constant temperature pool, an experiment tube (3), a preheating tube (2) and a liquid storage tube (4) are all arranged in a water bath pool (61), a constant temperature heating component is arranged in the water bath pool (61), and a water outlet (63) and a water drainage switch (62) are arranged on the water bath pool (61).
7. The smooth muscle testing system of claim 5, wherein: the experimental tube (3), the preheating tube (2) and the liquid storage tube (4) are all placed on the electric heating plate.
8. The smooth muscle testing system of claim 1, wherein: the experimental tube comprises a waste liquid box (5), wherein the waste liquid box (5) is embedded on a shell, and the bottom of the experimental tube (3) is communicated with the waste liquid box (5) through a liquid discharge tube and a water pump.
9. The smooth muscle testing system of claim 1, wherein: the fixing piece (72) consists of a fixing body (a 3) and two knobs, wherein an opening hole below the fixing body (a 3) is inserted into the top of the automatic lifting rod (8) and is fixed by the second knob (a 2); the side surface of the fixing body (a 3) is opened, the tension transducer (71) is embedded in the opening, and the tension transducer is fixed by the first knob (a 1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322122033.0U CN220812450U (en) | 2023-08-08 | 2023-08-08 | Smooth muscle experiment system capable of accurately controlling preload |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322122033.0U CN220812450U (en) | 2023-08-08 | 2023-08-08 | Smooth muscle experiment system capable of accurately controlling preload |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220812450U true CN220812450U (en) | 2024-04-19 |
Family
ID=90702597
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322122033.0U Active CN220812450U (en) | 2023-08-08 | 2023-08-08 | Smooth muscle experiment system capable of accurately controlling preload |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220812450U (en) |
-
2023
- 2023-08-08 CN CN202322122033.0U patent/CN220812450U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209546716U (en) | A kind of toy organ room temperature machine perfusion system | |
| CN220812450U (en) | Smooth muscle experiment system capable of accurately controlling preload | |
| CN107296664A (en) | A kind of magnetic, multi-functional animal experimental table of one man operation | |
| CN111420151B (en) | Portable enema device | |
| CN209933607U (en) | Ascites drainage device for department of gastroenterology | |
| CN110638411A (en) | Clamping and supporting device of medical endoscope | |
| CN205556644U (en) | Separation heart perfusion device suitable for rat | |
| CN211445185U (en) | Biochemical experimental device | |
| CN221230609U (en) | Automatic liquid supplementing device for water bath kettle | |
| CN208448195U (en) | A kind of Renal biospy uses constant temperature Iodophor heating device | |
| CN112717224A (en) | Indirect enema device for hysterectomy | |
| CN207562949U (en) | A kind of rack for test tube for flocculant performance test | |
| CN205925753U (en) | Homothermal water bath | |
| CN206594103U (en) | A kind of blood vessel cavitation effect experimental provision | |
| CN217187236U (en) | Traditional Chinese medicine retention enema bag | |
| CN215305340U (en) | Traditional chinese medical science nursing is with lavipeditum bucket | |
| CN110917669A (en) | Biochemical experimental device and experimental method using same | |
| CN216877401U (en) | Urinary surgery washes drainage bag with bladder | |
| CN216573184U (en) | Tibetan medicine urinalysis heater | |
| CN220083313U (en) | Gastric lavage liquid temperature control device | |
| CN213557191U (en) | Urine test-tube rack | |
| CN217796220U (en) | Novel test tube water bath heating device | |
| CN115607756A (en) | Back-transfusion filter device after bile external drainage | |
| CN214232250U (en) | Clinical drainage device of ICU severe patients | |
| CN219846518U (en) | Constant temperature water heater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |