CN220812431U - Self-contained biological indicator structure - Google Patents
Self-contained biological indicator structure Download PDFInfo
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- CN220812431U CN220812431U CN202322498564.XU CN202322498564U CN220812431U CN 220812431 U CN220812431 U CN 220812431U CN 202322498564 U CN202322498564 U CN 202322498564U CN 220812431 U CN220812431 U CN 220812431U
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- China
- Prior art keywords
- cavity
- top cover
- self
- biological indicator
- glass tube
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- 239000000090 biomarker Substances 0.000 title claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 46
- 238000000502 dialysis Methods 0.000 claims abstract description 35
- 239000001963 growth medium Substances 0.000 claims abstract description 31
- 239000011521 glass Substances 0.000 claims abstract description 28
- 238000011084 recovery Methods 0.000 claims abstract description 9
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 4
- 239000001888 Peptone Substances 0.000 claims abstract description 4
- 108010080698 Peptones Proteins 0.000 claims abstract description 4
- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000008103 glucose Substances 0.000 claims abstract description 4
- 235000019319 peptone Nutrition 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000005388 borosilicate glass Substances 0.000 claims description 6
- 239000003365 glass fiber Substances 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 description 16
- 238000004659 sterilization and disinfection Methods 0.000 description 16
- 230000008859 change Effects 0.000 description 5
- 238000009434 installation Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000010079 rubber tapping Methods 0.000 description 2
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 description 1
- 241000193410 Bacillus atrophaeus Species 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000009516 primary packaging Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012414 sterilization procedure Methods 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
Abstract
The utility model discloses a self-contained biological indicator structure, which comprises a cavity and a glass tube arranged in the cavity, wherein a recovery culture medium bromocresol purple glucose peptone water culture medium is sealed in the glass tube; a carrier is placed at the top of the glass tube, and spores of geobacillus stearothermophilus are placed on the carrier; a piece of dialysis material is arranged at the top end of the cavity, a top cover is arranged above the dialysis material, and the top cover is pressed downwards to cover the upper end of the cavity, so that the upper end of the cavity is wrapped by the dialysis material to complete assembly. The utility model is characterized in that the carrier containing spores and the glass tube containing the recovery culture medium are contained in the primary package together, and the dialysis material is isolated from the outside to prevent pollution; the operation is simple, and the inoculation can be realized only by using the special clamp to clamp the glass tube to make the culture medium contact with the carrier when in use, thereby avoiding possible pollution in the inoculation process.
Description
Technical Field
The utility model belongs to the technical field of image processing, and particularly relates to a self-contained biological indicator structure.
Background
A biological indicator is a particular microbial preparation that has a defined and stable resistance to a particular sterilization procedure.
Disadvantages of the prior art:
The gas sterilization test requires the use of biological indicators for effect monitoring. It is now common for the biological indicator to be of a split structure, i.e. the carrier is separated from the recovery medium, the carrier being packaged in a primary packaging bag and the recovery medium being held in a separate container. After sterilization of the biological indicator, the carrier needs to be transferred and inoculated into a vessel containing a culture medium for culture, so that pollution is easy to cause in the transfer process, and false positive occurs. The operation is complex, and the required working time is long.
Disclosure of utility model
The utility model aims to solve the defects and the shortcomings in the prior art, and provides a self-contained biological indicator structure which is formed by jointly containing a carrier containing spores and a glass tube containing a recovery culture medium in a primary package, isolating the dialysis material from the outside, preventing pollution, and having simple operation, wherein the glass tube is clamped by a special clamp, so that the culture medium is contacted with the carrier, and the inoculation can be realized, thereby avoiding the possible pollution in the inoculation process.
The technical scheme adopted for solving the technical problems is as follows: a self-contained biological indicator structure comprises a cavity and a glass tube arranged in the cavity, wherein a recovery culture medium bromocresol purple glucose peptone water culture medium is sealed in the glass tube; a carrier is placed at the top of the glass tube, and spores of geobacillus stearothermophilus are placed on the carrier; a piece of dialysis material is arranged at the top end of the cavity, a top cover is arranged above the dialysis material, and the top cover is pressed downwards to cover the upper end of the cavity, so that the upper end of the cavity is wrapped by the dialysis material to complete assembly.
Preferably, the top cover is pressed down to a half of the height of the top cover to cover the upper end of the cavity, so that the dialysis material wraps the upper end of the cavity to complete assembly, and the distance between the glass tube and the top of the cavity is 2mm-5mm.
The carrier is brought as close as possible to the dialysis material, and the concentration of the carrier accessory sterilization medium is increased rapidly.
Preferably, the cavity comprises a cavity body and a top cover mounting ring positioned at the top of the cavity body, the dialysis material is arranged on the top cover mounting ring, and the top cover is sleeved on the top cover mounting ring to tightly compress the dialysis material to wrap the top cover mounting ring.
The structure is convenient for the cover of the top cover to be tightly covered.
Preferably, the inside of top cap is equipped with the spacing collar of cooperation top cap installing ring, and the upper half fretwork of top cap side, the top of top cap is sealed.
The upper half part of the side surface of the top cover is hollowed out, so that a sterilizing medium can pass through the top cover conveniently; after sterilization, the top cover is required to be pressed for culture, and the top of the top cover is sealed and not hollowed out so as to prevent the culture medium from evaporating during culture.
Preferably, the carrier is stainless steel sheet, glass fiber paper or PVC sheet, the dialysis material is Teveqid strong dialysis material, and the glass tube is made of neutral borosilicate glass or low borosilicate glass.
Has better tolerance and air permeability to various gases.
Preferably, the outer diameter of the top cover mounting ring is larger than the outer diameter of the cavity, and an arc-shaped connecting part is arranged between the cavity and the top cover mounting ring.
The structure ensures that the appearance is attractive and elegant.
Preferably, the cavity body, the arc-shaped connecting portion and the top cover mounting ring are integrally formed, and the inner diameter of the cavity body, the inner diameter of the arc-shaped connecting portion and the inner diameter of the top cover mounting ring are the same.
Preferably, the top cover is made of PET, PC, PP, PVC or PE material, and the cavity is made of PET, PC, PP, PVC or PE material.
Preferably, the cavity is made of PET, PC, PP, PVC or PE material that is highly transparent and or highly ductile.
The color change of the culture medium is convenient to observe, and the cavity is not damaged and cracked when the glass tube is broken.
The utility model is characterized in that the carrier containing spores and the glass tube containing the recovery culture medium are contained in the primary package together, and the dialysis material is isolated from the outside to prevent pollution; the operation is simple, and the inoculation can be realized only by using the special clamp to clamp the glass tube to make the culture medium contact with the carrier when in use, thereby avoiding possible pollution in the inoculation process.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the utility model, and that other drawings can be obtained from these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present utility model;
in the figure, the device comprises a cavity 1, a glass tube 2, a carrier 3, a dialysis material 4, a top cover 5, a top cover mounting ring 6, a limit ring 7 and an arc-shaped joint part 8.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Example 1
As shown in FIG. 1, the self-contained biological indicator structure comprises a cavity 1 and a glass tube 2 arranged in the cavity 1, wherein a recovery culture medium bromocresol purple glucose peptone water culture medium is sealed in the glass tube 2; a piece of carrier 3 is placed at the top of the glass tube, and spores of geobacillus stearothermophilus are placed on the carrier 3; a piece of dialysis material 4 is arranged at the top end of the cavity 1, a top cover 5 is arranged above the dialysis material, and the top cover 5 is pressed downwards to cover the upper end of the cavity 1, so that the dialysis material wraps the upper end of the cavity 1 to complete assembly. The top cover 5 is pressed down to the height of one half of the top cover 5 to cover the upper end of the cavity 1, so that the dialysis material 4 wraps the upper end of the cavity to complete assembly, and the distance between the glass tube 2 and the top of the cavity 1 is 2mm-5mm. The cavity 1 includes cavity main part and is located the top cap installation circle 6 at cavity main part top, dialysis material 4 sets up on top cap installation circle 6, top cap 5 cover compresses tightly the parcel top cap installation circle 6 with dialysis material 4 on top cap installation circle 6. The inside of top cap 5 is equipped with the spacing collar 7 of cooperation top cap installation circle 6, and the upper half fretwork of top cap 5 side, and the top of top cap 5 is sealed. The carrier 3 is made of stainless steel sheets, glass fiber paper or PVC sheets, the dialysis material is made of Tevex strong dialysis material, and the glass tube 2 is made of neutral borosilicate glass or low borosilicate glass. The external diameter of the top cover mounting ring 6 is larger than the external diameter of the cavity 1, and an arc-shaped connecting part 8 is arranged between the cavity 1 and the top cover mounting ring 6. The cavity body, the arc-shaped connecting part 8 and the top cover mounting ring 6 are integrally formed, and the inner diameter of the cavity body, the inner diameter of the arc-shaped connecting part 8 and the inner diameter of the top cover mounting ring 6 are the same. The top cover 5 is made of PET, PC, PP, PVC or PE material, and the cavity 1 is made of PET, PC, PP, PVC or PE material.
Example 2
The cavity 1 is made of PET, PC, PP, PVC or PE material with high transparency and high toughness.
Example 3
The cavity 1 is made of PET, PC, PP, PVC or PE material with high transparency or high toughness.
The working principle of the utility model is as follows: the self-contained biological indicator is placed in a vaporized hydrogen peroxide sterilization environment for sterilization, and a sterilization medium enters the cavity through dialysis material at the top of the cavity to kill spores on the carrier. After sterilization, the top cover is fully pressed down to seal the cavity, and a special clamp is used for clamping the middle part of the cavity to break the glass tube and enable the culture medium to flow out. Observing whether the carrier is completely soaked in the culture medium, and if the carrier is not completely soaked, tapping the cavity to enable the carrier to be completely soaked in the culture medium. Transferring the self-contained biological indicator to an incubator, culturing according to a specified condition, observing whether the culture medium changes color after the culturing is finished to judge whether bacteria grow, wherein the change of the culture medium to yellow indicates the bacteria growth, which indicates the sterilization failure, and the non-color change of the culture medium indicates the sterile growth, which indicates the sterilization success.
On the basis of the above, the microorganism is replaced by bacillus atrophaeus, and the culture medium is replaced by bromothymol blue trypticase culture medium. The working principle is as follows: the self-contained biological indicator is placed into an ethylene oxide sterilization environment for sterilization, and sterilization medium enters the cavity through dialysis material at the top of the cavity to kill spores on the carrier. After sterilization, the top cover is fully pressed down to seal the cavity, and a special clamp is used for clamping the middle part of the cavity to break the glass tube and enable the culture medium to flow out. Observing whether the carrier is completely soaked in the culture medium, and if the carrier is not completely soaked, tapping the cavity to enable the carrier to be completely soaked in the culture medium. Transferring the self-contained biological indicator to an incubator, culturing according to a specified condition, observing whether the culture medium changes color after culturing is finished to judge whether bacteria grow, wherein the change of the culture medium to yellow indicates the bacteria growth, which indicates that sterilization fails, and the non-color change of the culture medium indicates the bacteria-free growth, which indicates that sterilization is successful.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims.
Claims (9)
1. A self-contained biological indicator structure characterized by: comprises a cavity (1) and a glass tube (2) arranged in the cavity (1), wherein a recovery culture medium bromocresol purple glucose peptone water culture medium is sealed in the glass tube (2); a piece of carrier (3) is placed at the top of the glass tube, and spores of geobacillus stearothermophilus are placed on the carrier (3); a piece of dialysis material (4) is arranged at the top end of the cavity (1), a top cover (5) is arranged above the dialysis material, and the top cover (5) is downwards pressed to cover the upper end of the cavity (1), so that the upper end of the cavity (1) is wrapped by the dialysis material to complete assembly.
2. The self-contained biological indicator structure of claim 1, wherein: the top cover (5) is pressed downwards until one half of the top cover (5) is sleeved on the upper end of the cavity (1), so that the dialysis material (4) wraps the upper end of the cavity to complete assembly, and the distance between the glass tube (2) and the top of the cavity (1) is 2mm-5mm.
3. The self-contained biological indicator structure of claim 1, wherein: the dialysis device is characterized in that the cavity (1) comprises a cavity main body and a top cover mounting ring (6) positioned at the top of the cavity main body, the dialysis material (4) is arranged on the top cover mounting ring (6), and the top cover (5) is sleeved on the top cover mounting ring (6) to tightly press the dialysis material (4) to wrap the top cover mounting ring (6).
4. The self-contained biological indicator structure of claim 2, wherein: the inside of top cap (5) is equipped with spacing collar (7) of cooperation top cap installing collar (6), and the upper half fretwork of top cap (5) side, and the top of top cap (5) is sealed.
5. The self-contained biological indicator structure of claim 1, wherein: the carrier (3) is made of stainless steel sheets, glass fiber paper or PVC sheets, the dialysis material is made of Teveqiao dialysis material, and the glass tube (2) is made of neutral borosilicate glass or low borosilicate glass.
6. A self-contained biological indicator structure according to claim 3, characterized in that: the outer diameter of the top cover mounting ring (6) is larger than that of the cavity (1), and an arc-shaped connecting part (8) is arranged between the cavity (1) and the top cover mounting ring (6).
7. The self-contained biological indicator structure of claim 6, wherein: the cavity body, the arc-shaped connecting portion (8) and the top cover mounting ring (6) are integrally formed, and the inner diameter of the cavity body, the inner diameter of the arc-shaped connecting portion (8) and the inner diameter of the top cover mounting ring (6) are identical.
8. The self-contained biological indicator structure of claim 1, wherein: the top cover (5) is made of PET, PC, PP, PVC or PE materials, and the cavity (1) is made of PET, PC, PP, PVC or PE materials.
9. The self-contained biological indicator structure of claim 8, wherein: the cavity (1) is made of PET, PC, PP, PVC or PE material with high transparency and or high toughness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322498564.XU CN220812431U (en) | 2023-09-14 | 2023-09-14 | Self-contained biological indicator structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322498564.XU CN220812431U (en) | 2023-09-14 | 2023-09-14 | Self-contained biological indicator structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220812431U true CN220812431U (en) | 2024-04-19 |
Family
ID=90679351
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322498564.XU Active CN220812431U (en) | 2023-09-14 | 2023-09-14 | Self-contained biological indicator structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220812431U (en) |
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2023
- 2023-09-14 CN CN202322498564.XU patent/CN220812431U/en active Active
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| Date | Code | Title | Description |
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| GR01 | Patent grant |