CN220709169U - Colloidal gold kit for detecting paratyphoid B antigen - Google Patents
Colloidal gold kit for detecting paratyphoid B antigen Download PDFInfo
- Publication number
- CN220709169U CN220709169U CN202322255210.2U CN202322255210U CN220709169U CN 220709169 U CN220709169 U CN 220709169U CN 202322255210 U CN202322255210 U CN 202322255210U CN 220709169 U CN220709169 U CN 220709169U
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- colorimetric
- window
- test paper
- paratyphoid
- antigen
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 title abstract description 6
- 238000001514 detection method Methods 0.000 claims abstract description 59
- 238000012360 testing method Methods 0.000 claims abstract description 32
- 239000000427 antigen Substances 0.000 claims description 12
- 102000036639 antigens Human genes 0.000 claims description 12
- 108091007433 antigens Proteins 0.000 claims description 12
- 238000003908 quality control method Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000003086 colorant Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims 4
- 238000011161 development Methods 0.000 abstract description 21
- 230000007547 defect Effects 0.000 abstract description 2
- 238000011179 visual inspection Methods 0.000 abstract description 2
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 231100000517 death Toxicity 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010022653 Intestinal haemorrhages Diseases 0.000 description 1
- 206010028813 Nausea Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000531795 Salmonella enterica subsp. enterica serovar Paratyphi A Species 0.000 description 1
- 241000577483 Salmonella enterica subsp. enterica serovar Paratyphi B Species 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 206010022694 intestinal perforation Diseases 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000003126 m-cell Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008693 nausea Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Abstract
The utility model discloses a colloidal gold kit for detecting a paratyphoid B antigen, which aims to solve the defect that whether a color development result is positive or negative is difficult to judge by naked eyes under the condition of not using a color comparison card in the prior art, and solves the technical problems by the following technical scheme: comprising the following steps: the test paper card comprises a test paper card outer shell and a test paper strip, wherein the test paper strip is arranged in the test paper card outer shell; the test paper card outer shell comprises an upper shell and a lower shell, wherein the upper shell is sequentially provided with a sample adding port and a reading window along the length direction of the upper shell, the side edge of the reading window is provided with a colorimetric window corresponding to the reading window, and a colorimetric structure is arranged in the colorimetric window; by arranging the colorimetric structure, when the detection result is interpreted, the detection result is compared with the colorimetric structure, so that the detection result can be accurately interpreted; the problem of inaccurate interpretation results caused by visual inspection of the detection results only is prevented.
Description
Technical Field
The utility model relates to the technical field of antigen kits, in particular to a colloidal gold kit for detecting paratyphoid B antigen.
Background
Paratyphoid B is a bacterial infection caused by salmonella paratyphi B, transmitted by ingestion of contaminated food and water. 2100 tens of thousands of cases and 22.2 tens of thousands of related deaths are estimated to occur annually worldwide. After ingestion of salmonella paratyphi, they enter the small intestine through the microfold cells, through which bacteria migrate to the mesenteric lymph nodes and multiply. Mainly manifested by fever, nausea, constipation and diarrhea, severe symptoms can lead to intestinal bleeding and perforation of the intestines and even death.
Common paratyphoid B detection methods include laboratory detection such as blood routine and fat up test, pathological detection and the like, but the methods are required to have expensive large-scale instruments and equipment, special laboratories, long time consumption, high detection cost and the like, and are not suitable for being applied to rapid screening and community level detection.
Therefore, in the prior art, a colloidal gold kit is used for detecting paratyphoid b, but after the colloidal gold kit is loaded, a period of time is required to observe the color development intensity of the detection line T to determine whether the detection line T is positive. If the external timer is not used, it is difficult to judge whether the time is within the interpretation time, the time is short, and the combination time is insufficient, so that false negative results can be caused, and the validity period of reading is exceeded, so that false positives can be caused.
If the color development result of the detection line T is critical between positive and negative, it is difficult for naked eyes to judge whether the color development result is positive or negative without using a color chart.
Therefore, in order to overcome the above-mentioned shortcomings, it is highly desirable to design a rapid, accurate, readable and time-indicating colloidal gold kit.
Disclosure of Invention
The utility model overcomes the defects in the prior art in the following aspects: (1) It is difficult to interpret the detection result in the correct interpretation time range; (2) Under the condition that a color chart is not used, whether a color development result is positive or negative is difficult to judge by naked eyes, and the colloidal gold kit for detecting the paratyphoid B antigen has a time prompt function, so that a detection result can be interpreted within an accurate time, and the detection result is prevented from being inaccurate because the detection time is too short or exceeds a reading effective period; by means of the color chart, the detection result can be accurately judged.
In order to solve the technical problems, the utility model adopts the following technical scheme: a colloidal gold kit for detecting paratyphoid b antigen, comprising: the test paper card comprises a test paper card outer shell and a test paper strip, wherein the test paper strip is arranged in the test paper card outer shell; the test paper card shell body includes casing and lower casing, and the last casing has set gradually application of sample mouth and reading window along its length direction, and the side of reading window is provided with the colorimetric window rather than corresponding, is provided with the colorimetric structure in the colorimetric window.
According to the utility model, the colorimetric structure is arranged, and when the detection result is interpreted, the detection result is compared with the colorimetric structure, so that the detection result can be accurately interpreted; the problem of inaccurate interpretation results caused by visual inspection of the detection results only is prevented.
Preferably, the upper shell is also provided with a reading prompt window, and a time reminding device is arranged in the reading prompt window.
By setting the time reminding device, the device has a time reminding function, so that the detection result can be interpreted in accurate time, and the detection result is prevented from being inaccurate because the detection time is too short or exceeds the reading effective period.
Preferably, the colorimetric structure is a roll-type colorimetric card, the roll-type colorimetric card comprises a colorimetric column rotationally arranged in a colorimetric window, the cylindrical surface of the colorimetric column is higher than the surface of the upper shell, and a plurality of colorimetric lines with different colors are arranged on the circumferential direction of the cylindrical surface of the colorimetric column.
When the detection result is interpreted, the colorimetric column is rotated, so that the colorimetric lines with different colors can be compared with the detection result, and the detection result is accurately interpreted.
Preferably, the test strip is provided with a quality control line C and a detection line T, and the colorimetric window corresponds to the detection line T in position.
The colorimetric window corresponds to the position of the detection line T, so that the colorimetric line can correspond to the detection line T, and the detection result can be conveniently and accurately judged.
Preferably, the axis of the cuvette is arranged along the width direction of the cuvette.
The structure enables the colorimetric wire to be transversely aligned with the detection wire T, so that a user can judge the result of the detection wire T more conveniently and accurately.
Preferably, the time reminding device is a pressable indicator light.
After the indicator lamp is pressed down, the indicator lamp is lighted up to be timed, the color of the indicator lamp is changed from red to green after 5 minutes, the indicator lamp can be read, if the time is 15 minutes, the color of the indicator lamp is changed from green to red, and the purpose of the design is to add a readable indicator, so that a user can know whether the time is within the interpretation time or not by observing the color of the indicator lamp without timing through an external tool after the sample is added.
Preferably, the test strip comprises an NC film and a PVC bottom plate arranged at the bottom of the NC film, wherein a gold mark pad and a sample pad are sequentially arranged on the left side of the NC film, and a water absorption pad is arranged on the right side of the NC film; the sample pad corresponds to the position of the sample adding port.
Compared with the prior art, the utility model has the beneficial effects that:
(1) By arranging the rotatable roll-type color chart, the detection result can be conveniently compared with a color comparison line on the roll-type color chart, so that the detection result can be accurately judged;
(2) By setting the time reminding device, the detection result can be interpreted in accurate time, and the detection result is prevented from being inaccurate because the detection time is too short or exceeds the reading effective period.
Drawings
FIG. 1 is a perspective view of the present utility model;
FIG. 2 is a schematic diagram of the structure of the test strip of the present utility model;
FIG. 3 is a top view of the upper housing structure of the present utility model;
in the figure: 1. sample adding port 2, reading window 3, colorimetric window 4, reading prompt window 5, upper shell, 6, lower shell, 8, test strip, 9, sample pad 10, gold mark pad 11, NC film 12, water absorbing pad 13, detection line T,14, quality control line C,15, roll-type color chart, 16, indicator lamp, 17, PVC bottom plate, 18, color line.
Detailed Description
The technical scheme of the utility model is further specifically described by the following specific embodiments with reference to the accompanying drawings:
referring to fig. 1 to 3, a colloidal gold kit for detecting paratyphoid b antigen includes a test strip card outer housing and a test strip 8, the test strip 8 being disposed in the test strip card outer housing; the test paper card shell body includes casing 5 and lower casing 6, and casing 5 has set gradually application of sample mouth 1 and reading window 2 along its length direction on, and the side of reading window 2 is provided with the colorimetric window 3 rather than corresponding, is provided with the colorimetric structure in the colorimetric window 3. The upper shell 5 is also provided with a reading prompt window 4, and a time reminding device is arranged in the reading prompt window 4.
The test strip 8 comprises an NC film 11 and a PVC bottom plate 17 arranged at the bottom of the NC film 11, a gold mark pad 10 and a sample pad 9 are sequentially bonded on the left side of the NC film 11, and a water absorption pad 12 is bonded on the right side of the NC film 11. One end of the sample pad 9 is placed on the gold-labeled pad 10, one end of the gold-labeled pad 10 is placed on the left side of the NC film 11, and one end of the water-absorbent pad 12 is placed on the right side of the NC film 11. The gold mark pad 10 corresponds to the sample adding port 1, an NC film 11 in the test strip 8 comprises a quality control line C14 and a detection line T13, the middle area of the NC film 11 comprising the quality control line C14 and the detection line T13 faces the reading window 2, and a color development strip of the detection line T13 is arranged along the width direction of the reading window 2.
The gold-labeled pad 10 is coated with a paratyphoid B monoclonal antibody and goat anti-mouse colloidal gold particles, the detection line T13 of the NC film 11 is coated with the paratyphoid B monoclonal antibody, the quality control line C14 of the NC film 11 is coated with mouse IgG, and when a sample is detected, if the sample contains paratyphoid B antigen, the antigen in the sample is combined with the colloidal gold particles coated with paratyphoid B monoclonal antibody-1 on the gold-labeled pad 10 to form a colloidal gold paratyphoid B antigen-antibody complex; then, the antigen is combined with mouse IgG coated on a detection line T13 of the NC film 11 and a quality control line C14 to form two clearly visible color development strips.
The colorimetric window 3 sets up in the side position that corresponds with detection line T13, the colorimetric structure is roll type colorimetric card 15, roll type colorimetric card 15 is including rotating the colorimetric column that sets up in the colorimetric window 3, the cylinder surface of colorimetric column is higher than the surface of last casing 5, be provided with the colorimetric line 18 of a plurality of different colours on the circumferencial direction of the face of cylinder of colorimetric column, the axis of colorimetric column sets up along the width direction of reading window 2, thereby make colorimetric line 18 can transversely align with detection line T13, make the user can more convenient, more accurate judgement detection line T13's result.
In order to be able to more accurately interpret the color development results, the cylindrical surface of the color comparison column is respectively provided with 10 color comparison lines 18 with different color development intensities according to a certain distance, color development intensity serial numbers are marked beside each color comparison line 18 with different intensities, the color development intensities of the color comparison lines 18 are arranged in descending order from the color development intensity serial number 10 to the color development intensity serial number 1, the color comparison line 18 corresponding to the color development intensity serial number 10 is strongest, the color comparison line 18 corresponding to the color development intensity serial number 1 is weakest, and when the result is interpreted, the color development intensity of the detection line T13 and the color development intensity serial number of the color comparison line 18 corresponding to the color comparison line are positive and less than or equal to 3, so that the user can rotate the roll-type color comparison card 15 better, and whether the color development results meet the negative or positive conditions is better interpreted.
The time alert device is a depressible indicator light 16. After the indicator light 16 is pressed down, the indicator light 16 lights up to a red light and starts timing, the color of the indicator light 16 changes from red to green after 5 minutes, the indication can be read, if the time passes by 15 minutes, the color of the indicator light 16 changes from green to red, and the purpose of the design is to add a readable indicator, so that a user can know whether the interpretation time is within only by observing the color of the indicator light 16 without timing through an external tool after sample application.
The general detection reagent only has two areas of a sample adding hole and a reading hole, and because the colloidal gold reagent has strict requirements on the reading time, operators generally need to prepare a timer beside the sample adding time to count the time, and then prepare a reading card to compare the color development intensity of a detection line.
According to the utility model, by adding the indicator lamp 16 beside the colorimetric window 3, an operator presses the indicator lamp 16 after finishing sample addition, the lamp turns red, the indicator lamp 16 turns green after 5 minutes, the time for interpretation is represented, and the indicator lamp 16 turns red after 15 minutes, the interpretation is inhibited, so that the detection result can be interpreted within effective time, and the inaccuracy of the detection result caused by too short detection time or the fact that the detection time exceeds the reading effective period is prevented.
And through being provided with 10 colour comparison lines 18 of different intensity at rotatable reel formula colour comparison card 15 to be provided with 10 intensity serial numbers respectively, during the reading, if colour development intensity of detection line T13 and colour development intensity serial number of colour comparison line 18 that corresponds are greater than 3, are positive, be less than or equal to 3 and be negative for the result of detection can be accurately interpreted.
The above-described embodiments are merely preferred embodiments of the present utility model, and the present utility model is not limited in any way, and other variations and modifications may be made without departing from the technical aspects set forth in the claims.
Claims (7)
1. A colloidal gold kit for detecting paratyphoid b antigen, comprising: the test paper card comprises a test paper card outer shell and a test paper strip, wherein the test paper strip is arranged in the test paper card outer shell; the test paper card shell body includes casing and lower casing, and the last casing has set gradually application of sample mouth and reading window along its length direction, and the side of reading window is provided with the colorimetric window rather than corresponding, is provided with the colorimetric structure in the colorimetric window.
2. The colloidal gold kit for detecting paratyphoid antigen as defined in claim 1, wherein the upper housing is further provided with a reading prompt window, and a time reminding device is arranged in the reading prompt window.
3. The colloidal gold kit for detecting paratyphoid antigen according to claim 1 or 2, wherein the colorimetric structure is a roll-type colorimetric card, the roll-type colorimetric card comprises a colorimetric column rotatably arranged in a colorimetric window, the cylindrical surface of the colorimetric column is higher than the surface of the upper shell, and a plurality of colorimetric lines of different colors are arranged in the circumferential direction of the cylindrical surface of the colorimetric column.
4. The colloidal gold kit for detecting paratyphoid antigen of claim 3, wherein the test strip is provided with a quality control line C and a detection line T, and the colorimetric window corresponds to the detection line T in position.
5. A colloidal gold kit for detecting paratyphoid antigen as defined in claim 3, wherein the axis of the colorimetric column is arranged along the width direction of the colorimetric window.
6. The colloidal gold kit for detecting paratyphoid antigen as defined in claim 2, wherein the time alert means is a depressible indicator light.
7. The colloidal gold kit for detecting paratyphoid b antigen according to claim 1 or 2, wherein the test strip comprises an NC membrane and a PVC bottom plate provided at the bottom of the NC membrane, a gold-labeled pad and a sample pad are sequentially provided on the left side of the NC membrane, and a water absorbing pad is provided on the right side of the NC membrane; the sample pad corresponds to the position of the sample adding port.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322255210.2U CN220709169U (en) | 2023-08-22 | 2023-08-22 | Colloidal gold kit for detecting paratyphoid B antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322255210.2U CN220709169U (en) | 2023-08-22 | 2023-08-22 | Colloidal gold kit for detecting paratyphoid B antigen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220709169U true CN220709169U (en) | 2024-04-02 |
Family
ID=90445992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322255210.2U Active CN220709169U (en) | 2023-08-22 | 2023-08-22 | Colloidal gold kit for detecting paratyphoid B antigen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220709169U (en) |
-
2023
- 2023-08-22 CN CN202322255210.2U patent/CN220709169U/en active Active
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