CN217443368U - Chinese medicine aflatoxin B 1 Colloidal gold immunochromatography rapid detection card - Google Patents
Chinese medicine aflatoxin B 1 Colloidal gold immunochromatography rapid detection card Download PDFInfo
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- CN217443368U CN217443368U CN202122923004.5U CN202122923004U CN217443368U CN 217443368 U CN217443368 U CN 217443368U CN 202122923004 U CN202122923004 U CN 202122923004U CN 217443368 U CN217443368 U CN 217443368U
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Abstract
The utility model discloses an aflatoxin B1 colloidal gold immunochromatography rapid detection card in traditional Chinese medicine, which comprises a test strip and a shell, wherein the test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane and absorbent paper; the device comprises a shell and a detection device, wherein the shell comprises an upper shell and a lower shell, the upper shell is provided with an observation window opposite to a nitrocellulose membrane and a sample liquid treatment pipe opposite to a sample pad, the upper surface of the sample liquid treatment pipe is fixedly provided with a liquid inlet, a double-layer filtering membrane is fixed inside the sample liquid treatment pipe, an accommodating cavity is arranged in the sample liquid treatment pipe and close to the lower part of the double-layer filtering membrane, and a colloidal gold marked aflatoxin B1 monoclonal antibody is freeze-dried in the accommodating cavity; a layer of water-soluble film is arranged below the containing cavity, and a disc-shaped fixing plate is clamped below the water-soluble film. The utility model can effectively filter impurities such as pigment in the traditional Chinese medicine by arranging the double-layer filtering membrane, thereby reducing interference and improving accuracy and sensitivity; meanwhile, the colloidal gold labeled antibody is assembled with the test strip, so that the operation steps can be simplified, the operation error is reduced, and the stable and accurate detection result is ensured.
Description
Technical Field
The utility model relates to a colloidal gold immunochromatography detects technical field, especially relates to an aflatoxin B in traditional chinese medicine 1 Colloidal gold immunochromatography rapid detection card.
Background
Aflatoxin B 1 (AflatoxinB 1 ，AFB 1 ) The aflatoxin is a toxin which is generated mainly by aspergillus flavus and aspergillus parasiticus, has the advantages of widest distribution, largest harm, closest relationship with human health and highest pollution level, and is widely applied to agricultural products and foods such as peanuts, corns, rice, wheat, nuts and the like. In addition, the traditional Chinese medicinal materials are extremely easy to mildew due to proper temperature and humidity in the storage process, so that various mycotoxins having toxic effects on human bodies are generated. Aflatoxin B 1 Even very small concentrations in humans and animals can lead to hepatotoxicity, teratogenicity, mutagenicity and carcinogenicity. Therefore, the aflatoxin B in the traditional Chinese medicinal materials is high-efficiency, sensitive and stable 1 The detection method is extremely important.
At present, the kit is commonly used for detecting aflatoxin B 1 The method comprises thin layer chromatography, enzyme linked immunosorbent assay, high performance liquid chromatography, colloidal gold immunochromatography, high performance liquid chromatography-tandem mass spectrometry, fluorescence immunoassay and the like, wherein the colloidal gold immunochromatography is the most rapid and convenient method. The colloidal Gold Immunochromatography (GICA) is used as a solid-phase membrane immunoassay, has the advantages of simple and convenient operation steps, no site limitation, no need of large-scale instruments and equipment, capability of obtaining a detection result by visual observation of detection personnel and the like, is widely applied to detection of the mycotoxins in the traditional Chinese medicinal materials, and is very suitable for rapid detection and screening in a sampling site.
Detection of aflatoxin B by colloidal gold immunochromatography 1 There are many reports at home and abroad, and commercial test paper strips are available. However, because the chemical components in the traditional Chinese medicine are complex and the matrix effect is seriously interfered, most samples also contain pigments, and the colloidal gold is light pink on a test strip, so that the subjective judgment is easily influenced by the color change of the test strip which is shielded by the interference of the background to be tested, and the false positive or false negative state is generated. Meanwhile, in order to improve the sensitivity of the existing test strip product, the colloidal gold labeled antibody is freeze-dried in the micropores, so that the colloidal gold labeled antibody can be fully contacted and fully reacted with a sample in the detection process, and the error is reduced. However, for such products, the sample solution needs to be added into the micropores to react with the gold-labeled antibody, and then the reacted solution is dropped on the test strip for detection, so that the solution is taken twice, the operation is complicated, and the operation error is easily increased.
SUMMERY OF THE UTILITY MODEL
The purpose of the utility model is to solve the problem of aflatoxin B in the traditional Chinese medicine in the prior art 1 The colloidal gold immunochromatographic test strip is easy to be interfered by impurities such as pigments and the like to mask the color change of the colloidal gold immunochromatographic test strip, so that subjective judgment is influenced, false positive or false negative is generated, and in a reaction mode of a gold-labeled micropore-test strip, liquid is taken twice, the operation is complicated, the operation error is increased, and the problem of inaccurate detection result is caused.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
chinese medicine aflatoxin B 1 The colloidal gold immunochromatographic assay rapid detection card comprises a test strip and a shell, wherein the test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane and absorbent paper, the sample pad, the nitrocellulose membrane and the absorbent paper are sequentially arranged on the bottom plate, a detection line is fixedly arranged on one side, close to the sample pad, of the nitrocellulose membrane, and a quality control line is fixedly arranged on one side, close to the absorbent pad, of the nitrocellulose membrane; the casing includes casing and lower casing, it is provided with just to the observation window of nitrocellulose membrane and just to the sample liquid treatment pipe of sample pad to go up the casing, the last fixed surface of sample liquid treatment pipe has seted up the inlet, the inside of sample liquid treatment pipe is fixed with double-deck filtration membrane, and the inside of sample liquid treatment pipe is close to double-deck filtration membraneA containing cavity is arranged below the box body, and colloidal gold labeled aflatoxin B is freeze-dried in the containing cavity 1 A monoclonal antibody; a layer of water-soluble film is arranged below the containing cavity, and a disc-shaped fixing plate is clamped below the water-soluble film.
The utility model discloses an among the colloidal gold immunity chromatography short-term test card, the bottom plate of test paper strip is PVC or other stereoplasm non-absorbent material, and the sample pad is glass fiber or polyester fiber.
The utility model discloses an among the colloidal gold immunity chromatography short-term test card, the inside fixed double-deck filtration membrane of appearance liquid treatment tube is one kind and can pass through aflatoxin B 1 The material of (1).
The utility model discloses an among the colloidal gold immunity chromatography short-term test card, double-deck filtration membrane is nylon membrane, polyvinylidene fluoride membrane, mixed cellulose ester membrane, polypropylene membrane, polyethersulfone membrane or polytetrafluoroethylene membrane.
The utility model discloses an among the colloidal gold immunity chromatography short-term test card, the diameter of water-soluble film is greater than the diameter that holds the chamber.
In the colloidal gold immunochromatographic rapid detection card of the present invention, the water-soluble film is a starch film or a polyvinyl alcohol film.
The utility model discloses an among the colloidal gold immunity chromatography short-term test card, the fixed plate edge is equipped with a plurality of connecting rod around the fixed plate center, goes up and corresponds the connecting rod on the casing and be equipped with a plurality of spread groove.
The utility model discloses an among the colloidal gold immunity chromatography short-term test card, the distance between two connecting rods of fixed plate symmetry is unanimous with the diameter of water-soluble film, and has seted up the drain hole on it, and the diameter in drain hole is unanimous with the diameter that holds the chamber.
The utility model discloses an among the colloidal gold immunity chromatography short-term test card, the lower surface of fixed plate and the lower surface parallel and level of last casing.
The utility model discloses a Chinese medicine aflatoxin B 1 The principle of colloidal gold immunochromatographic detection card is that after traditional Chinese medicine samples are pretreated, a sample liquid is pushed into a sample liquid treatment tube by an injectorIn a liquid inlet with the surface opened, the sample liquid passes through the double-layer filtering membrane under the pressure and then enters the accommodating cavity to be marked with the aflatoxin B by the freeze-dried colloidal gold in the accommodating cavity 1 Reacting the monoclonal antibody; the reaction solution dissolves the water-soluble film, and then falls onto the test strip sample pad through the liquid guide hole of the fixing plate and moves forwards under the action of capillary; aflatoxin B in samples 1 Aflatoxin B on detection line arranged on nitrocellulose membrane of test strip in moving process 1 -carrier protein conjugate competitively binds to colloidal gold labeled aflatoxin B 1 The monoclonal antibody makes the area show a certain color, and whether aflatoxin B remains in the traditional Chinese medicine sample can be judged by visual observation or by using a corresponding reading instrument 1 。
Compared with the prior art, the utility model discloses following beneficial effect has:
1) through setting up double-deck filtration membrane, can carry out effective filtration to impurities such as pigment in the traditional chinese medicine to reduce the interference, improve degree of accuracy and sensitivity.
2) By labeling the aflatoxin B with colloidal gold 1 The monoclonal antibody is freeze-dried in the accommodating cavity, and the gold-labeled antibody can be fully contacted with a sample and fully reacted in the detection process, so that the error is reduced, and the reaction sensitivity of the whole system is improved; meanwhile, the colloidal gold labeled antibody is assembled with the test strip, so that the operation steps can be simplified, the operation error is reduced, and the stable and accurate detection result is ensured.
Drawings
FIG. 1 is a side view of the colloidal gold immunochromatographic rapid test card of the present invention;
FIG. 2 is a top view of the colloidal gold immunochromatographic rapid test card of the present invention;
FIG. 3 is a cross-sectional view of the colloidal gold immunochromatographic rapid detection card of the present invention;
FIG. 4 is a partial cross-sectional view of the colloidal gold immunochromatographic rapid detection card of the present invention;
fig. 5 is a sectional view of the test strip of the present invention.
In the figure: 10. a test strip; 11. a base plate; 12. sample (I)A pad; 13. a nitrocellulose membrane; 131. detecting lines; 132. a quality control line; 14. absorbent paper; 20. a housing; 21. an upper housing; 211. an observation window; 212. a sample liquid processing tube; 213. a liquid inlet; 214. a filtration membrane; 215. a filtration membrane; 216. an accommodating chamber; 217. colloidal gold labeled aflatoxin B 1 A monoclonal antibody; 218. connecting grooves; 22. a lower housing; 30. a water-soluble film; 40. a fixing plate; 41. a connecting rod; 42. and a liquid guide hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-5, a Chinese medicine aflatoxin B 1 The colloidal gold immunochromatographic rapid detection card comprises a test strip 10 and a shell 20, wherein the test strip 10 is placed in the shell 20. Specifically, the test strip 10 includes a bottom plate 11, a sample pad 12, a nitrocellulose membrane 13 and absorbent paper 14, the sample pad 12, the nitrocellulose membrane 13 and the absorbent paper 14 are sequentially disposed on the bottom plate 11, a detection line 131 is fixedly disposed on one side of the nitrocellulose membrane 13 close to the sample pad 12, and a quality control line 132 is fixedly disposed on one side close to the absorbent paper 14. The housing 20 comprises an upper housing 21 and a lower housing 22, wherein an observation window 211 and a sample solution processing tube 212 are arranged on the upper housing 21, the observation window 211 is arranged right opposite to the nitrocellulose membrane 13, and the sample solution processing tube 212 is arranged right opposite to the sample pad 12. A liquid inlet 213 is fixedly arranged on the upper surface of the sample liquid treatment pipe 212, a double-layer filtering membrane, namely a filtering membrane 214 and a filtering membrane 215, is fixedly arranged inside the sample liquid treatment pipe 212, a containing cavity 216 is arranged inside the sample liquid treatment pipe 212 and close to the filtering membrane 215, and the colloidal gold marked aflatoxin B is lyophilized in the containing cavity 216 1 A monoclonal antibody 217; a layer of water-soluble film 30 is arranged below the accommodating cavity 216, and a disc-shaped fixing plate 40 is clamped below the water-soluble film 30.
In this embodiment, the bottom plate 11 of the test strip 10 is made of PVC or other hard non-absorbent material, and preferably, the bottom plate 11 is made of PVC; the sample pad 12 is made of glass fiber or polyester fiber, and preferably, the material of the sample pad 12 is glass fiber.
In this embodiment, in order to filter impurities such as pigments in the traditional Chinese medicine, a double-layer filtering membrane, i.e., a filtering membrane 214 and a filtering membrane 215, is fixed inside the sample solution treating tube 212; to facilitate detection of aflatoxin B 1 The content, the filter membrane 214 and the filter membrane 215 are one which can pass aflatoxin B 1 Preferably, the filtering membrane 214 and the filtering membrane 215 are nylon membrane, polyvinylidene fluoride membrane, mixed cellulose ester membrane, polypropylene membrane, polyethersulfone membrane or polytetrafluoroethylene membrane, more preferably, the filtering membrane 214 is mixed cellulose ester membrane and the filtering membrane 215 is polyethersulfone membrane.
In this embodiment, aflatoxin B is labeled with the lyophilized colloidal gold in the receiving chamber 216 in order to contact the sample liquid 1 After reacting, the monoclonal antibody 217 is dripped on the sample pad 12, a layer of water-soluble film 30 is arranged below the containing cavity 216, and the diameter of the water-soluble film 30 is larger than that of the containing cavity 216; to facilitate dissolution of the film, the water-soluble film 30 is a starch film or a polyvinyl alcohol film, and preferably, the water-soluble film 30 is a polyvinyl alcohol film.
In this embodiment, in order to fix the water-soluble film 30, a disc-shaped fixing plate 40 is clamped below the water-soluble film 30, a plurality of connecting rods 41 are arranged around the center of the fixing plate 40 at the edge of the fixing plate 40, a plurality of connecting slots 218 are arranged on the upper shell 21 corresponding to the connecting rods 41, and the distance between two symmetrical connecting rods of the fixing plate 40 is consistent with the diameter of the water-soluble film 30.
In this embodiment, aflatoxin B is labeled with the lyophilized colloidal gold in the receiving chamber 216 in order to contact the sample liquid 1 The monoclonal antibody 217 reacts and dissolves the water-soluble film 30, and then drops on the sample pad 12, the fixing plate 40 is provided with a liquid guide hole 42, and the diameter of the liquid guide hole 42 is consistent with that of the accommodating cavity 216.
In this embodiment, in order to make the upper case 21 and the lower case 22 of the case 20 be tightly screwed together, the lower surface of the fixing plate 40 is flush with the lower surface of the upper case 21.
The utility model discloses a theory of operation and use flow: after the traditional Chinese medicine sample is pretreated, the sample liquid is pushed into a liquid inlet 213 arranged on the upper surface of a sample liquid treatment tube 212 by an injector, the sample liquid passes through a filtering membrane 214 and a filtering membrane 215 in sequence under pressure, and the double-layer filtering membrane can effectively filter impurities such as pigment in the traditional Chinese medicine sample liquid and can not prevent aflatoxin B 1 The passage of (a); the sample liquid passing through the double-layer filtering membrane enters the accommodating cavity 216 and is marked with aflatoxin B by freeze-dried colloidal gold in the sample liquid 1 The monoclonal antibody 217 reacts, the reaction solution dissolves the water-soluble film 30, and then falls onto the sample pad 12 of the test strip 10 through the liquid guide hole 42 of the fixing plate 40, and then diffuses onto the nitrocellulose membrane 13 of the test strip 10, and finally the sample solution is absorbed and removed through the absorbent paper 14 of the test strip 10.
A detection line 131 is fixedly arranged on one side, close to the sample pad 12, of the nitrocellulose membrane 13 of the test strip 10, and a quality control line 132 is fixedly arranged on one side, close to the absorbent paper 14; the detection line 131 is coated with aflatoxin B 1 Conjugate of carrier protein, sample solution and lyophilized colloidal gold labeled aflatoxin B in holding chamber 216 1 After the monoclonal antibody 217 reacts, the aflatoxin B in the sample flows through a detection line 131 1 Will be in contact with aflatoxin B on detection line 131 1 -carrier protein conjugate competitively binds to colloidal gold labeled aflatoxin B 1 The monoclonal antibody and the surplus colloidal gold label will continue to chromatography, and bind with the goat anti-mouse secondary antibody fixed on the quality control line 132, finally the detection line 131 and the quality control line 132 will show red bands.
If the color development of the detection line is deeper than that of the quality control line or is consistent with that of the quality control line, the aflatoxin B in the sample is shown 1 The residue of (A) is less than the detection limit, and is negative; if the color ratio of the detection line is light or the detection line is not colored, the result shows that the aflatoxin B in the sample 1 The residual amount of (A) is higher than the detection limit, and the result is positive.
Although embodiments of the present invention have been shown and described, it would be appreciated by those skilled in the art that changes may be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the claims and their equivalents.
Claims (9)
1. Chinese medicine aflatoxin B 1 Colloidal gold immunity chromatography short-term test card, including test paper strip and casing, its characterized in that: the test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane and absorbent paper, wherein the sample pad, the nitrocellulose membrane and the absorbent paper are sequentially arranged on the bottom plate, a detection line is fixedly arranged on one side, close to the sample pad, of the nitrocellulose membrane, and a quality control line is fixedly arranged on one side, close to the absorbent pad, of the nitrocellulose membrane; the casing includes casing and lower casing, it is provided with just to the observation window of nitrocellulose membrane and just to the sample liquid processing pipe of sample pad to go up the casing, the last fixed surface who manages is handled to the sample liquid has seted up the inlet, the inside that the pipe was handled to the sample liquid is fixed with double-deck filtration membrane, and the inside that the pipe was handled to the sample liquid is equipped with near double-deck filtration membrane and holds the chamber, it has colloidal gold mark aflatoxin B to freeze-dry in the chamber to hold 1 A monoclonal antibody; a layer of water-soluble film is arranged below the containing cavity, and a disc-shaped fixing plate is clamped below the water-soluble film.
2. The colloidal gold immunochromatographic rapid detection card according to claim 1, which comprises: the bottom plate of the test strip is made of PVC, and the sample pad is made of glass fiber or polyester fiber.
3. The colloidal gold immunochromatographic rapid detection card according to claim 1, which comprises: the double-layer filtering membrane fixed in the sample liquid treatment tube can pass through aflatoxin B 1 The material of (1).
4. The colloidal gold immunochromatographic rapid detection card according to claim 3, which is characterized in that: the double-layer filtering membrane is a nylon membrane, a polyvinylidene fluoride membrane, a mixed cellulose ester membrane, a polypropylene membrane, a polyether sulfone membrane or a polytetrafluoroethylene membrane.
5. The colloidal gold immunochromatographic rapid detection card according to claim 1, which comprises: the diameter of the water-soluble film is larger than that of the containing cavity.
6. The colloidal gold immunochromatographic rapid detection card according to any one of claims 1 or 5, wherein: the water-soluble film is a starch film or a polyvinyl alcohol film.
7. The colloidal gold immunochromatographic rapid detection card according to claim 1, which comprises: the fixed plate edge is equipped with a plurality of connecting rod around the fixed plate center, goes up the casing and is equipped with a plurality of spread groove corresponding to the connecting rod.
8. The colloidal gold immunochromatographic rapid detection card according to claim 7, which comprises: the distance between two symmetrical connecting rods of the fixing plate is consistent with the diameter of the water-soluble film, a liquid guide hole is formed in the fixing plate, and the diameter of the liquid guide hole is consistent with that of the containing cavity.
9. The colloidal gold immunochromatographic rapid detection card according to claim 1, which comprises: the lower surface of the fixing plate is flush with the lower surface of the upper shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122923004.5U CN217443368U (en) | 2021-11-26 | 2021-11-26 | Chinese medicine aflatoxin B 1 Colloidal gold immunochromatography rapid detection card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122923004.5U CN217443368U (en) | 2021-11-26 | 2021-11-26 | Chinese medicine aflatoxin B 1 Colloidal gold immunochromatography rapid detection card |
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| CN217443368U true CN217443368U (en) | 2022-09-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122923004.5U Active CN217443368U (en) | 2021-11-26 | 2021-11-26 | Chinese medicine aflatoxin B 1 Colloidal gold immunochromatography rapid detection card |
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- 2021-11-26 CN CN202122923004.5U patent/CN217443368U/en active Active
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