CN220703720U - Fibronectin cell culture device - Google Patents
Fibronectin cell culture device Download PDFInfo
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- CN220703720U CN220703720U CN202322291857.0U CN202322291857U CN220703720U CN 220703720 U CN220703720 U CN 220703720U CN 202322291857 U CN202322291857 U CN 202322291857U CN 220703720 U CN220703720 U CN 220703720U
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- 102000016359 Fibronectins Human genes 0.000 title claims abstract description 33
- 108010067306 Fibronectins Proteins 0.000 title claims abstract description 33
- 238000004113 cell culture Methods 0.000 title claims description 12
- 238000001514 detection method Methods 0.000 claims abstract description 17
- 230000000903 blocking effect Effects 0.000 claims abstract description 4
- 238000005096 rolling process Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims description 8
- 238000012258 culturing Methods 0.000 abstract description 12
- 239000001963 growth medium Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 241001474374 Blennius Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010036595 Premature delivery Diseases 0.000 description 1
- -1 acryl Chemical group 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000003320 cell separation method Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a fibronectin cell culturing device, which comprises: the base is vertically arranged relative to the tabletop, and an annular limiting groove is formed in the base; the cover body is arranged on the base, the outer wall of the cover body is integrally formed with a flange, the flange is positioned in the limit groove, a plurality of balls distributed at equal intervals are embedded on the flange, and the balls can be arranged in the limit groove in a rolling way; the cover body is also provided with a detection hole, and a blocking cover is arranged in the detection hole. The fibronectin cell culturing device provided by the embodiment of the utility model can avoid excessive contact between the sample and the external environment, so that factors influencing the development of the sample are reduced, and the probability of pollution of the sample is reduced.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a fibronectin cell culture device.
Background
The fibronectin-containing tissue sample may be obtained by cell separation and culture methods, which generally involve cell separation of the tissue sample, culture and passage through appropriate culture conditions, to obtain fibronectin cells.
In the related art, during the cultivation process of fibronectin cells, samples need to be detected or culture medium is supplemented at certain intervals, and when the samples are taken and placed, the samples are excessively contacted with the external environment, so that factors influencing the development of the samples are increased, and the samples are easily polluted.
Disclosure of Invention
The present utility model aims to solve at least to some extent one of the technical problems in the above-described technology.
Therefore, an object of the present utility model is to provide a fibronectin cell culturing apparatus, which can avoid excessive contact between a sample and the external environment, thereby reducing factors affecting the development of the sample and reducing the probability of contamination of the sample.
To achieve the above object, a first aspect of the present utility model provides a fibronectin cell culturing apparatus comprising: the base is vertically arranged relative to the tabletop, and an annular limiting groove is formed in the base; the cover body is arranged on the base, a flange is integrally formed on the outer wall of the cover body, the flange is positioned in the limiting groove, a plurality of balls distributed at equal intervals are embedded on the flange, and the balls can be arranged in the limiting groove in a rolling mode; the cover body is also provided with a detection hole, and a blocking cover is arranged in the detection hole.
According to the fibronectin cell culturing device, a relatively closed space is formed by the cover body and the base, the detection hole is formed in the cover body, the self-priming tube is conveniently detected to extend into the cover body, the cover body is rotated, the plurality of balls roll in the limiting groove, so that the position of the self-priming tube is changed, a plurality of samples are conveniently detected or culture medium is conveniently supplemented, the number of times that the samples are exposed to the external environment is reduced, factors influencing the development of the samples are reduced, and the probability of pollution of the samples is reduced.
In addition, the fibronectin cell culturing device provided by the utility model can also have the following additional technical characteristics:
specifically, a plurality of grooves are formed in the base and located in the cover body, and a cultivation box is placed in the grooves.
Specifically, a heating rod is arranged on the base, and a temperature and humidity sensor is arranged on the inner wall of the cover body.
Specifically, the blanking cover is provided with a vent hole, and a separation net is arranged in the vent hole.
Specifically, the outer wall and the upper part of the cover body are respectively provided with a visual window.
Additional aspects and advantages of the utility model will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the utility model.
Drawings
The foregoing and/or additional aspects and advantages of the utility model will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:
FIG. 1 is a schematic diagram showing the structure of a fibronectin cell culture apparatus according to an embodiment of the present utility model;
FIG. 2 is a schematic view showing the structure of a base of a fibronectin cell culture apparatus according to one embodiment of the utility model;
FIG. 3 is a schematic cross-sectional structure of a fibronectin cell culture apparatus according to one embodiment of the utility model.
Reference numerals: 1. a base; 11. a limit groove; 2. a cover body; 21. a flange; 22. a ball; 23. a detection hole; 24. a blanking cover; 31. a groove; 32. a cultivating box; 41. heating rod; 42. a temperature and humidity sensor; 51. a vent hole; 52. a screen; 61. and a visual window.
Detailed Description
Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.
The following describes a fibronectin cell culturing apparatus according to an embodiment of the present utility model with reference to the accompanying drawings.
The fibronectin cell culturing device provided by the utility model is mainly used for culturing the fibronectin cell sample after separating the tissue sample containing the fibronectin cells so as to obtain the fibronectin cells, and can be used for wound repair, cancer diagnosis, premature delivery prediction and the like in medicine.
As shown in fig. 1 and 2, the fibronectin cell culturing apparatus according to the embodiment of the present utility model may include: a base 1 and a cover body 2.
Wherein, base 1 is placed perpendicularly for the desktop, has seted up annular spacing groove 11 on the base 1.
It should be noted that, the bottom of the base 1 described in this embodiment is provided with a plurality of supporting legs, and the supporting legs can reduce the contact area between the base 1 and the table top, so as to increase the pressure and increase the stability of the device when in use.
The cover body 2 is arranged on the base 1, a flange 21 is integrally formed on the outer wall of the cover body 2, the flange 21 is positioned in the limit groove 11, a plurality of balls 22 distributed at equal intervals are embedded on the flange 21, the balls 22 can be arranged in the limit groove 11 in a rolling mode, the cover body 2 is further provided with a detection hole 23, and a blocking cover 24 is arranged in the detection hole 23.
It should be noted that, in this embodiment, the detection hole 23 and the plug cover 24 are connected by interference fit.
In addition, the bottom of the cover 2 described in this embodiment is attached to the inner bottom wall of the limit groove 11.
In one embodiment of the present utility model, as shown in fig. 2, the base 1 is provided with a plurality of grooves 31, and is located inside the cover 2, and the cultivation box 32 is placed inside the grooves 31.
It should be noted that the number of the grooves 31 described in this embodiment may be 3, 4, 5, etc., and is not particularly limited herein.
It can be appreciated that setting up recess 31 can be spacing to cultivating box 32, avoids cultivating box 32 to remove at will, and the separation setting is convenient for take out the sample after cultivating between cultivating box 32 and the base 1 to and wash cultivating box 32, thereby make things convenient for the use of this device.
Specifically, after separating a tissue sample (e.g., seaweed or animal tissue, etc.) containing fibronectin cells, a relevant technician obtains a fibronectin cell sample, divides it into a plurality of aliquots, sequentially places the aliquots into a plurality of culturing cartridges 32, and places the cover 2 on the base 1.
The device is started, a sample of fibronectin cells enters the incubation stage, after a preset time interval (the preset time can be determined according to the actual situation), a related person opens the blanking cover 24, inserts the detection self-priming tube into the detection hole 23, stretches the end of the detection self-priming tube into an incubation box 32 for sampling, then withdraws the self-priming tube, extrudes the sample onto an external glass slide, and then determines the content of the fibronectin cells by means of an instrument (microscope) and records the content.
The related technician rotates the cover body 2, and the cover body 2 drives the balls 22 to roll in the limiting groove 11, so that the position of the detection hole 23 is opposite to the other cultivation box 32, and the steps are repeated to finish sampling a plurality of samples. The method avoids excessive contact between the sample and the external environment, reduces factors influencing the development of the sample, and reduces the probability of pollution of the sample.
The relevant technician can supplement the culture medium in the incubator 32 according to the plurality of sets of sample data, thereby ensuring the normal development of the fibronectin cells, and after the detection or the supplement of the culture medium is finished, the relevant technician can close the blanking cover 24 so that the samples continue to develop.
In one embodiment of the present utility model, as shown in fig. 3, a heating rod 41 is provided on the base 1, and a temperature and humidity sensor 42 is provided on the inner wall of the cover 2.
In the embodiment of the utility model, external commercial power supplies power to a heating rod 41 and a temperature and humidity sensor 42 in the device, the heating rod 41 is connected with the external commercial power, and the temperature and humidity sensor 42 is connected with the heating rod 41.
It should be noted that, in this embodiment, the heating rod 41 is erected at the center of the base 1, so that the environment in the cover 2 can be heated, thereby avoiding the incubator 32 from being excessively close to the heat source, and ensuring a suitable living environment for the fibronectin cells.
In one embodiment of the present utility model, as shown in fig. 3, the vent hole 51 is formed in the cover 24, and the barrier net 52 is disposed inside the vent hole 51.
It should be noted that the spacer web 52 described in this embodiment is made of two nonwoven fabrics and one meltblown fabric.
It can be understood that the vent hole 51 is arranged to facilitate the external air to enter the cover body 2, thereby ensuring the breath of the sample cells, and the separation net 52 is arranged to ensure that external foreign matters are prevented from entering the cover body 2 through the vent hole 51 under the premise of ventilation, so that the sample is prevented from being polluted.
In one embodiment of the present utility model, as shown in fig. 1, the outer wall and the upper portion of the cover 2 are respectively provided with a viewing window 61.
It should be noted that, the viewing window 61 described in this embodiment may be made of a transparent acryl material.
It will be appreciated that the person skilled in the art can observe the development of the sample in the incubator 32 inside the housing 2 through the viewing window 61 and also facilitate sampling through the self-priming tube.
As a possible case, in order to make it easier for the relevant technician to sample through the self-priming tube, the whole of the cover 2 can be made of transparent acrylic material.
In summary, the fibronectin cell culturing device provided by the utility model forms a relatively closed space through the cover body and the base, and the cover body is provided with the detection hole, so that the self-priming tube can be conveniently detected to extend into the cover body, the cover body is rotated, and the plurality of balls roll in the limit groove, so that the position of the self-priming tube is changed, a plurality of samples can be conveniently detected or culture medium can be conveniently supplemented, the number of times that the samples are exposed in the external environment is reduced, factors influencing the development of the samples are reduced, and the probability of pollution of the samples is reduced.
In the description of this specification, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present utility model, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
While embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the utility model.
Claims (5)
1. A fibronectin cell culture device, comprising: a base (1) and a cover body (2), wherein,
the base (1) is vertically arranged relative to the tabletop, and an annular limit groove (11) is formed in the base (1);
the cover body (2) is arranged on the base (1), a flange (21) is integrally formed on the outer wall of the cover body (2), the flange (21) is positioned in the limit groove (11), a plurality of balls (22) distributed at equal intervals are embedded on the flange (21), and the balls (22) can be arranged in the limit groove (11) in a rolling mode;
the cover body (2) is also provided with a detection hole (23), and a blocking cover (24) is arranged in the detection hole (23).
2. The fibronectin cell culture device according to claim 1, wherein the base (1) is provided with a plurality of grooves (31) and is positioned inside the cover (2), and a culture box (32) is arranged inside the grooves (31).
3. The fibronectin cell culture device according to claim 1, wherein a heating rod (41) is arranged on the base (1), and a temperature and humidity sensor (42) is arranged on the inner wall of the cover body (2).
4. The fibronectin cell culture device according to claim 1, wherein the plug cover (24) is provided with a vent hole (51), and a screen (52) is arranged inside the vent hole (51).
5. The fibronectin cell culture device according to claim 1, wherein the outer wall and the upper portion of the cover (2) are respectively provided with a viewing window (61).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322291857.0U CN220703720U (en) | 2023-08-25 | 2023-08-25 | Fibronectin cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322291857.0U CN220703720U (en) | 2023-08-25 | 2023-08-25 | Fibronectin cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220703720U true CN220703720U (en) | 2024-04-02 |
Family
ID=90439575
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322291857.0U Active CN220703720U (en) | 2023-08-25 | 2023-08-25 | Fibronectin cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220703720U (en) |
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2023
- 2023-08-25 CN CN202322291857.0U patent/CN220703720U/en active Active
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