CN210294086U - Illumination and dark treatment device for measuring superoxide dismutase sample - Google Patents
Illumination and dark treatment device for measuring superoxide dismutase sample Download PDFInfo
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- CN210294086U CN210294086U CN201920883808.7U CN201920883808U CN210294086U CN 210294086 U CN210294086 U CN 210294086U CN 201920883808 U CN201920883808 U CN 201920883808U CN 210294086 U CN210294086 U CN 210294086U
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Abstract
The application relates to an illumination and dark treatment device for measuring a superoxide dismutase sample, which comprises a plurality of light-transmitting centrifuge tube frames and at least one light-tight centrifuge tube frame which are arranged in a reaction space of the illumination and dark treatment device, wherein the reaction space comprises a box body and a box cover; in addition, light sources are arranged around the light-transmitting centrifuge tube shelves and the light-tight centrifuge tube shelves, and meanwhile, the light sources are connected with a power supply module through a first switch, wherein the power supply module supplies power for the illumination and dark processing device for measuring the superoxide dismutase samples through being connected with an external power supply. Therefore, the illumination treatment and the dark treatment can be simultaneously finished in the power supply of the illumination and dark treatment device for the superoxide dismutase sample determination, the illumination treatment time is controlled through the first switch, two devices are not needed to be used for respectively carrying out the illumination treatment and the dark treatment, and the flow of biochemical experiments is simplified.
Description
Technical Field
The application relates to the technical field of biochemical equipment, in particular to an illumination and dark treatment device for measuring a superoxide dismutase sample.
Background
In the biochemical field, samples are often subjected to measurement, and some samples require light treatment and dark treatment during the measurement.
At present, in such a sample measurement process requiring illumination and dark processing, illumination and dark processing are generally performed by using an illumination processing apparatus and a dark processing apparatus, respectively, and the sample measurement process is further complicated by using apparatuses corresponding to two opposite reaction conditions.
SUMMERY OF THE UTILITY MODEL
To overcome, at least to some extent, the problems of the related art, the present application provides an illumination and dark treatment device for the determination of superoxide dismutase samples.
The illumination and dark treatment device for detecting the superoxide dismutase sample comprises:
a box body and a box cover; the box body and the box cover form a reaction space of the illumination and dark treatment device for the superoxide dismutase sample determination;
a plurality of light-transmitting centrifuge tube shelves and at least one light-tight centrifuge tube shelf are arranged in the reaction space; the light-transmitting centrifuge tube rack and the light-tight centrifuge tube rack are used for placing centrifuge tubes with reaction liquid according to the requirements of the biochemical reaction experiment; the centrifugal pipe orifice is arranged in a light-tight way;
light sources are arranged around each light-transmitting centrifuge tube rack and each light-tight centrifuge tube rack;
the light source is connected with the power supply module through a first switch; the power supply module is used for connecting an external power supply to supply power for the illumination and dark treatment device for the superoxide dismutase sample determination.
Optionally, the device further comprises a light source bracket;
the light source bracket uniformly divides the reaction space into a plurality of regular spaces; the light sources are arranged at the periphery of each regular space and fixed on the light source bracket; the light-transmitting centrifuge tube shelves and the light-tight centrifuge tube shelves are correspondingly arranged in the regular spaces one by one.
Optionally, the light-transmitting centrifuge tube rack and the light-tight centrifuge tube rack are both provided with centrifuge tube slots for placing the centrifuge tubes;
the depth of the centrifugal tube groove is smaller than the height of the centrifugal tube.
Optionally, the outer perimeter of the cross section of each regular space close to the box cover side is matched with the outer perimeter of the cross section of the groove wall at the groove opening of the centrifugal pipe; so that when inserting the centrifuging tube that the centrifuge tube mouth set up for light tight translucent centrifuge tube holder or light tight centrifuge tube holder set up when in the regular space, the light that the light source in the regular space sent only shines in the regular space.
Optionally, the tube opening of the centrifuge tube shields external light through a light-tight cover.
Optionally, the box body is provided with a vent, and an exhaust fan is arranged at the vent;
the exhaust fan is connected with the power supply module through a second switch.
Optionally, the exhaust fan includes a fan motor and a motor speed regulator;
the motor speed regulator is connected with the power supply module through the second switch;
the motor speed regulator is provided with a speed regulating knob for regulating the rotating speed of the fan.
Optionally, a dimming module is arranged on a loop of the light source and used for adjusting the illumination intensity of the light source.
Optionally, the system further comprises a controller; the controller is respectively connected with the dimming module and the motor speed regulator.
Optionally, the light source further comprises a light intensity sensor arranged in the regular space and a temperature sensor arranged in the box body;
the light intensity sensor and the temperature sensor are connected with the controller, so that the controller controls the dimming module to adjust the brightness of the light source according to a light intensity signal detected by the light intensity sensor, and controls the motor speed regulator to adjust the rotating speed of the fan motor according to a temperature signal detected by the temperature sensor.
The technical scheme provided by the application can comprise the following beneficial effects: the method comprises the following steps that a plurality of light-transmitting centrifuge tube racks and at least one light-tight centrifuge tube rack are arranged in a reaction space of an illumination and dark treatment device, wherein the reaction space is composed of a box body and a box cover, the light-transmitting centrifuge tube racks and the light-tight centrifuge tube racks are used for placing centrifuge tubes with reaction liquid according to biochemical experiment requirements, and tube openings of the centrifuge tubes are arranged in a non-transparent mode; in addition, light sources are arranged around the light-transmitting centrifuge tube shelves and the light-tight centrifuge tube shelves, and meanwhile, the light sources are connected with a power supply module through a first switch, wherein the power supply module supplies power for the illumination and dark processing device for measuring the superoxide dismutase samples through being connected with an external power supply. Therefore, the illumination treatment and the dark treatment can be simultaneously finished in the power supply of the illumination and dark treatment device for the superoxide dismutase sample determination, the illumination treatment time is controlled through the first switch, two devices are not needed to be used for respectively carrying out the illumination treatment and the dark treatment, and the flow of biochemical experiments is simplified.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and together with the description, serve to explain the principles of the application.
FIG. 1 is a schematic structural diagram of an illumination and dark processing device for measuring a superoxide dismutase sample, provided in an embodiment of the present application.
Fig. 2 is a schematic structural diagram of a light source bracket according to a first embodiment of the present application.
Fig. 3 is a schematic structural diagram of an illumination and dark processing device for detecting a superoxide dismutase sample provided in this embodiment.
Reference numerals: a box body-1, a box cover-2, a centrifuge tube shelf-3, a light source-4, a light source bracket-5, a support rod-51, a ventilation opening-6, an exhaust fan-7 and an operation panel-8.
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present application. Rather, they are merely examples of apparatus consistent with certain aspects of the present application, as detailed in the appended claims.
In the biochemical field, samples are often measured, and some samples need to be subjected to light treatment and dark treatment in the measurement process, for example, for measuring the activity of superoxide dismutase, the light treatment is carried out by using light equipment, and the dark treatment is carried out by using a dark treatment device.
The living body is affected by various adverse environments in the whole growth and development process, such as extreme high temperature, water stress, atmospheric pollution and pathogenic bacteria invasion, which can cause cells to generate a large amount of active oxygen O2 -If the active oxygen cannot be removed in time, the living body will be damaged. Superoxide dismutase (SOD) is the first antioxidant enzyme in an active oxygen scavenging system and has very important function in the process of protecting cells from oxidative damage. Therefore, the measurement of biological SOD activity is one of important indicators for identifying whether an organism is stressed, how stress resistance of the organism is, and the effect of exogenous substances such as drugs on alleviating stress in the organism.
Since active oxygen itself is unstable and difficult to prepare, methods for measuring SOD generally employ indirect methods, except few direct methods. Nitro-tetrazolium chloride blue (NBT) reduction method and dianisidine method are indirect methods commonly used for measuring SOD activity in scientific research and production. In the measuring process, the illumination is needed for a certain time under 4000Lx sunlight. However, a light treatment device and a dark treatment device are required in the experimental process. The use of two opposing reaction conditions for the device makes the sample measurement process more complicated.
In order to solve the above problems, the present application provides an illumination and dark treatment apparatus for measuring a superoxide dismutase sample, which can be used in any biochemical experiment requiring both illumination and dark treatment for measuring the activity of superoxide dismutase. The following is a description of the specific structure of the light and dark treatment apparatus for measuring superoxide dismutase samples by way of example.
Example one
Referring to fig. 1, fig. 1 is a schematic structural diagram of an illumination and dark processing apparatus for detecting a superoxide dismutase sample according to an embodiment of the present application.
As shown in fig. 1, the illumination and dark processing device for detecting superoxide dismutase samples provided in this embodiment includes:
a box body 1 and a box cover 2; the box body and the box cover form a reaction space of the illumination and dark treatment device for the superoxide dismutase sample determination;
a plurality of light-transmitting centrifuge tube shelves and at least one light-tight centrifuge tube shelf are arranged in the reaction space; the light-transmitting centrifuge tube rack and the light-tight centrifuge tube rack are used for placing centrifuge tubes with reaction liquid according to the requirements of the biochemical reaction experiment; the tube opening of the centrifugal tube is light-tight; hereinafter, the centrifuge tube rack 3 refers to a light-transmitting centrifuge tube rack or a light-tight centrifuge tube rack;
the light source is connected with the power supply module through a first switch; the power supply module is used for connecting an external power supply to supply power for the illumination and dark treatment device for the superoxide dismutase sample determination.
The reaction space of the illumination and dark treatment device is internally provided with a plurality of light-transmitting centrifuge tube racks and at least one light-tight centrifuge tube rack, wherein the reaction space consists of a box body and a box cover, the light-transmitting centrifuge tube racks and the light-tight centrifuge tube racks are used for placing centrifuge tubes with reaction liquid according to the biochemical experiment requirements, and the tube openings of the centrifuge tubes are arranged in a non-transparent way; in addition, light sources are arranged around the light-transmitting centrifuge tube shelves and the light-tight centrifuge tube shelves, and meanwhile, the light sources are connected with a power supply module through a first switch, wherein the power supply module supplies power for the illumination and dark processing device for measuring the superoxide dismutase samples through being connected with an external power supply. Therefore, the illumination treatment and the dark treatment can be simultaneously finished in the power supply of the illumination and dark treatment device for the superoxide dismutase sample determination, the illumination treatment time is controlled through the first switch, two devices are not needed to be used for respectively carrying out the illumination treatment and the dark treatment, and the flow of biochemical experiments is simplified.
Further, the illumination and dark treatment device for measuring the superoxide dismutase sample in the embodiment can further comprise a light source bracket; the light source bracket uniformly divides the reaction space into a plurality of regular spaces; the light sources are arranged at the periphery of each regular space and fixed on the light source bracket; the light-transmitting centrifuge tube shelves and the light-tight centrifuge tube shelves are correspondingly arranged in the regular spaces one by one.
Fig. 2 may be referred to for a structure of the light source holder, and fig. 2 is a schematic structural diagram of a light source holder provided in an embodiment of the present application. As shown in fig. 2, the light source support 5 may be composed of a plurality of support rods 51, the regular space may be a rectangular parallelepiped space, and the support rods are sides of a rectangular parallelepiped regular space, the regular space has six faces, each face is composed of four support rods (the faces are spatially formed and may not have a solid body), and the central line or the central point of the other faces except the face close to the box cover may be provided with the light source.
It should be noted that a transparent centrifuge tube rack or a non-transparent centrifuge tube rack is correspondingly disposed in each regular space.
In addition, the light source may be in various forms, such as a strip of fluorescent lamps, a daylight bead or a light bar of daylight beads. The light-transmitting centrifuge tube rack can be made of light-transmitting glass materials or other materials with high light-transmitting performance, and the light-proof centrifuge tube rack can be made of light-proof materials such as black plastics or other materials with light-transmitting performance meeting the requirements. Hereinafter, the centrifuge tube holder refers to a light-transmitting centrifuge tube holder or a light-tight centrifuge tube holder.
In order to take out the centrifugal tube from the centrifuging tube frame conveniently, can set up the degree of depth of placing the centrifuging tube groove of centrifuging tube in the centrifuging tube frame to being less than the size of centrifuging tube height, like this, when placing the centrifuging tube in the centrifuging tube frame, the nozzle portion of centrifuging tube can be followed and is protruded in the centrifuging tube frame, and the experimenter can take out the centrifuging tube from the centrifuging tube frame through convex part.
Further, the outer perimeter of the cross section of each regular space close to the box cover side is matched with the outer perimeter of the cross section of the groove wall at the groove opening of the centrifugal pipe; so that when inserting the centrifuging tube that the centrifuge tube mouth set up for light tight translucent centrifuge tube holder or light tight centrifuge tube holder set up when in the regular space, the light that the light source in the regular space sent only shines in the regular space. On this basis, the orificial light-tight setting of centrifuging tube can be realized through light-tight lid, and light-tight lid can hide the part of centrifuge tube protrusion centrifuge tube rack completely, prevents that external light from getting into the centrifuging tube through the bulge. The light-tight cover can be made of light-tight materials such as black plastics and the like or materials with light-tight performance meeting the requirements.
In addition, the light emitted by the light source in the regular space is ensured to be only irradiated in the regular space, so that the light absorption amount in the reaction process can be effectively controlled.
Example two
Referring to fig. 3, fig. 3 is a schematic structural diagram of an illumination and dark processing apparatus for detecting a superoxide dismutase sample provided in this embodiment.
As shown in fig. 3, the illumination and dark processing device for detecting superoxide dismutase samples provided in this embodiment includes:
a box body 1 and a box cover 2; the box body and the box cover form a reaction space of the illumination and dark treatment device for the superoxide dismutase sample determination;
a plurality of light-transmitting centrifuge tube shelves and at least one light-tight centrifuge tube shelf are arranged in the reaction space; the light-transmitting centrifuge tube rack and the light-tight centrifuge tube rack are used for placing centrifuge tubes with reaction liquid according to the requirements of the biochemical reaction experiment; the tube opening of the centrifugal tube is light-tight; hereinafter, the centrifuge tube rack 3 refers to a light-transmitting centrifuge tube rack or a light-tight centrifuge tube rack;
a plurality of light sources 4 are arranged around each light-transmitting centrifuge tube rack and each light-tight centrifuge tube rack;
the light source is connected with the power supply module through a first switch; the power supply module is used for connecting an external power supply to supply power for the illumination and dark treatment device for the superoxide dismutase sample determination.
The reaction space of the illumination and dark treatment device is internally provided with a plurality of light-transmitting centrifuge tube racks and at least one light-tight centrifuge tube rack, wherein the reaction space consists of a box body and a box cover, the light-transmitting centrifuge tube racks and the light-tight centrifuge tube racks are used for placing centrifuge tubes with reaction liquid according to the biochemical experiment requirements, and the tube openings of the centrifuge tubes are arranged in a non-transparent way; in addition, light sources are arranged around the light-transmitting centrifuge tube shelves and the light-tight centrifuge tube shelves, and meanwhile, the light sources are connected with a power supply module through a first switch, wherein the power supply module supplies power for the illumination and dark processing device for measuring the superoxide dismutase samples through being connected with an external power supply. Therefore, the illumination treatment and the dark treatment can be simultaneously finished in the power supply of the illumination and dark treatment device for the superoxide dismutase sample determination, the illumination treatment time is controlled through the first switch, two devices are not needed to be used for respectively carrying out the illumination treatment and the dark treatment, and the flow of biochemical experiments is simplified.
In order to reduce the temperature in the illumination and dark treatment device for measuring the superoxide dismutase sample, the illumination and dark treatment device for measuring the superoxide dismutase sample of the embodiment can be provided with a ventilation opening 6 on the box body, and an exhaust fan 7 at the ventilation opening. Wherein, the vent can set up the ventilation bars structure to reduce the influence of external environment through the vent to reaction space. The exhaust fan is connected with the power supply module through the second switch so as to control the start and stop of the exhaust fan through the second switch.
In addition, the exhaust fan may include a fan motor and a motor governor; the motor speed regulator is connected with the power supply module through the second switch; the motor speed regulator is provided with a speed regulating knob for regulating the rotating speed of the fan. The light source loop can be also provided with a dimming module to adjust the illumination intensity of the light source.
Furthermore, the illumination and dark processing device for measuring the superoxide dismutase sample can also comprise a controller, and the controller can be respectively connected with the dimming module and the motor speed regulator. The controller may be a single chip microcomputer. The type of the singlechip can be, but is not limited to, MCS-51 series.
The illumination and dark treatment device for measuring the superoxide dismutase sample can also comprise a light intensity sensor arranged in the regular space and a temperature sensor arranged in the box body; the light intensity sensor and the temperature sensor are connected with the controller, so that the controller controls the dimming module to adjust the brightness of the light source according to a light intensity signal detected by the light intensity sensor, and controls the motor speed regulator to adjust the rotating speed of the fan motor according to a temperature signal detected by the temperature sensor. The signal of the motor governor may be, but is not limited to, US-52, SS-22, SS-32, etc.
The type of the temperature sensor may be, but is not limited to, PT100, and the light intensity sensor may be, but is not limited to, OPT 3001.
In addition, the illumination and dark processing device for measuring the superoxide dismutase sample can further comprise an operation panel 8, the first switch, the second switch and the speed regulation knob can be arranged on the operation panel, a touch screen can be further arranged on the operation panel, the touch screen is in communication connection with the controller, the illumination and dark processing device can be used for displaying a light intensity signal detected by the light intensity sensor, a temperature signal detected by the temperature sensor, illumination processing time and the like, and can also send signals to the controller through the touch screen to control the dimming module to adjust the light intensity and control the motor speed regulator to adjust the rotating speed of the fan motor.
It should be noted that the dimming module may be a sliding resistor, may be a light sensing resistor, or other structures capable of changing the resistor, and the light sources may be connected in parallel first, and then connected in series with the dimming module, the power supply module, and the first switch to form a light source loop.
The specific circuit connection among the controller, the temperature sensor, the light intensity sensor, the touch screen, the motor speed regulator and the dimming module can be realized according to the models by referring to the specification of the controller, and the detailed description is omitted here.
In addition, the correlation structures in the first embodiment and the second embodiment can be randomly combined according to requirements.
It is understood that the same or similar parts in the above embodiments may be mutually referred to, and the same or similar parts in other embodiments may be referred to for the content which is not described in detail in some embodiments.
It should be noted that, in the description of the present application, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Further, in the description of the present application, the meaning of "a plurality" means at least two unless otherwise specified.
In the description herein, reference to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
Although embodiments of the present application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present application, and that variations, modifications, substitutions and alterations may be made to the above embodiments by those of ordinary skill in the art within the scope of the present application.
Claims (10)
1. An illumination and dark treatment device for measuring a superoxide dismutase sample is applied to a biochemical reaction experiment and is characterized by comprising:
a box body and a box cover; the box body and the box cover form a reaction space of the illumination and dark treatment device for the superoxide dismutase sample determination;
a plurality of light-transmitting centrifuge tube shelves and at least one light-tight centrifuge tube shelf are arranged in the reaction space; the light-transmitting centrifuge tube rack and the light-tight centrifuge tube rack are used for placing centrifuge tubes with reaction liquid according to the requirements of the biochemical reaction experiment; the tube opening of the centrifugal tube is light-tight;
a plurality of light sources are arranged around each light-transmitting centrifuge tube rack and each light-tight centrifuge tube rack;
the light source is connected with the power supply module through a first switch; the power supply module is used for connecting an external power supply to supply power for the illumination and dark treatment device for the superoxide dismutase sample determination.
2. The light and dark treatment device for the superoxide dismutase sample determination as claimed in claim 1, further comprising a light source holder;
the light source bracket uniformly divides the reaction space into a plurality of regular spaces; the plurality of light sources are arranged at the periphery of each regular space and fixed on the light source bracket; the light-transmitting centrifuge tube shelves and the light-tight centrifuge tube shelves are correspondingly arranged in the regular spaces one by one.
3. The illumination and dark treatment device for superoxide dismutase sample determination as claimed in claim 2, wherein the light-transmitting centrifuge tube rack and the light-tight centrifuge tube rack are both provided with centrifuge tube slots for placing the centrifuge tubes;
the depth of the centrifugal tube groove is smaller than the height of the centrifugal tube.
4. The superoxide dismutase sample measuring light and dark treatment device as set forth in claim 3, wherein the outer perimeter of the cross section of each of the regular spaces on the side close to the cover matches the outer perimeter of the cross section of the groove wall at the groove opening of the centrifuge tube; so that when inserting the centrifuging tube that the centrifuge tube mouth set up for light tight translucent centrifuge tube holder or light tight centrifuge tube holder set up when in the regular space, the light that the light source in the regular space sent only shines in the regular space.
5. The apparatus for light and dark treatment of superoxide dismutase sample determination as claimed in claim 4, wherein the tube opening of the centrifuge tube is shielded from outside light by a light-tight cover.
6. The illumination and dark treatment device for detecting the superoxide dismutase sample according to the claim 2, characterized in that the box body is provided with a ventilation opening, and the ventilation opening is provided with an exhaust fan;
the exhaust fan is connected with the power supply module through a second switch.
7. The apparatus for light and dark treatment of superoxide dismutase sample determination as claimed in claim 6, wherein the exhaust fan comprises a fan motor and a motor governor;
the motor speed regulator is connected with the power supply module through the second switch;
the motor speed regulator is provided with a speed regulating knob for regulating the rotating speed of the fan.
8. The illumination and dark treatment device for detecting superoxide dismutase samples as claimed in claim 7, wherein a light adjusting module is arranged on the loop of the light source for adjusting the illumination intensity of the light source.
9. The light and dark treatment device for superoxide dismutase sample determination of claim 8, further comprising a controller; the controller is respectively connected with the dimming module and the motor speed regulator.
10. The apparatus for light and dark treatment of superoxide dismutase sample determination as claimed in claim 9, further comprising a light intensity sensor disposed in the regular space and a temperature sensor disposed inside the housing;
the light intensity sensor and the temperature sensor are connected with the controller, so that the controller controls the dimming module to adjust the brightness of the light source according to a light intensity signal detected by the light intensity sensor, and controls the motor speed regulator to adjust the rotating speed of the fan motor according to a temperature signal detected by the temperature sensor.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110118773A (en) * | 2019-06-13 | 2019-08-13 | 中国农业科学院棉花研究所 | The illumination of superoxide dismutase sample measurement and dark treatment device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110118773A (en) * | 2019-06-13 | 2019-08-13 | 中国农业科学院棉花研究所 | The illumination of superoxide dismutase sample measurement and dark treatment device |
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