CN220564583U - Stem cell exosome separation and extraction device - Google Patents
Stem cell exosome separation and extraction device Download PDFInfo
- Publication number
- CN220564583U CN220564583U CN202322058327.1U CN202322058327U CN220564583U CN 220564583 U CN220564583 U CN 220564583U CN 202322058327 U CN202322058327 U CN 202322058327U CN 220564583 U CN220564583 U CN 220564583U
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- fixedly connected
- stem cell
- heat preservation
- extraction device
- cell exosome
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 41
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 40
- 238000000926 separation method Methods 0.000 title claims abstract description 30
- 238000000605 extraction Methods 0.000 title claims abstract description 27
- 238000003756 stirring Methods 0.000 claims abstract description 39
- 230000001681 protective effect Effects 0.000 claims abstract description 14
- 238000012216 screening Methods 0.000 claims abstract description 11
- 238000004321 preservation Methods 0.000 claims description 32
- 230000000694 effects Effects 0.000 abstract description 7
- 238000001914 filtration Methods 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 abstract description 4
- 238000007599 discharging Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 3
- 230000003028 elevating effect Effects 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000035992 intercellular communication Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the field of cell separation and extraction, and discloses a stem cell exosome separation and extraction device which comprises a base, wherein the upper end of the base is fixedly connected with left and right opposite supporting columns, the upper end of each supporting column is fixedly connected with a platform, the upper end of each platform is fixedly connected with a protective sleeve, the front side of each protective sleeve is provided with an electric push rod, the rear end of each electric push rod is fixedly connected with a connecting rod, the rear end of each connecting rod is fixedly connected with a rack, the racks are in meshed connection with a gear, the left side of the gear is fixedly connected with a rotating rod, the inner side wall of the front end of each protective sleeve is provided with a sliding groove, the sliding grooves are in sliding connection with a sliding block, and the rear ends of the sliding blocks are fixedly connected with the racks. According to the utility model, through the mutual operation and coordination of the electric push rod, the rack, the gear, the rotating rod, the stirring plate, the screening layer and the filter box, the effect of stirring, separating and screening stem cells and then filtering and extracting is achieved, so that the extraction efficiency is accelerated.
Description
Technical Field
The utility model relates to the field of cell separation and extraction, in particular to a stem cell exosome separation and extraction device.
Background
Stem cells are self-renewing cells which have the ability to produce highly differentiated daughter cells from which all stem cells secrete exosomes which contain proteins and which may be present in the body fluids of the human body, and which play an important role in intercellular communication, thus, we generally need to isolate and extract exosomes from stem cells.
The existing separation and extraction device often obtains exosomes through centrifugal filtration mode, but because the concentration of stem cells is very high, has certain stickness, so this efficiency is not high, and the exosomes that the current separation and extraction device often can not be extracted are preserved properly and timely, cause losing temperature easily, finally lead to its deactivation, can not exert its effect.
Disclosure of Invention
In order to overcome the defects, the utility model provides a stem cell exosome separation and extraction device, which aims to improve the problems that the stem cell exosome separation and extraction device has low working efficiency of filtration and is not timely preserved and extracted in a constant temperature environment after separation to cause inactivation because the stem cell concentration is too high and has viscosity under normal conditions.
In order to achieve the above purpose, the present utility model adopts the following technical scheme: the utility model provides a stem cell exosome separation extraction element, includes the base, relative support column about base upper end fixedly connected with, support column upper end fixedly connected with platform, platform upper end fixedly connected with protective sheath, the protective sheath front side is provided with electric putter, electric putter rear end fixedly connected with connecting rod, connecting rod rear end fixedly connected with rack, rack meshing is connected with the gear, gear left side fixedly connected with dwang, the spout has been seted up to protective sheath front end inside wall, spout sliding connection has the slider, slider rear end fixedly connected with the rack.
As a further description of the above technical solution:
the middle part of the upper end of the base is provided with a stirring tank, and the outer side of the stirring tank is fixedly connected with a left rotating rod and a right rotating rod which are opposite.
As a further description of the above technical solution:
the stirring tank is characterized in that a rotating shaft is arranged in the middle of the inner side of the stirring tank, the left stirring rod and the right stirring rod are fixedly connected to the outer side of the rotating shaft, the stirring plate is fixedly connected to the inner side wall of the stirring tank, the left stirring plate and the right stirring plate are opposite, and a screening layer is arranged below the rotating shaft.
As a further description of the above technical solution:
the agitator tank upper end fixedly connected with motor, the motor is located the base top.
As a further description of the above technical solution:
the outside fixedly connected with front and back relative valve of agitator tank, the agitator tank front side is provided with the filter cartridge.
As a further description of the above technical solution:
the output port at the lower end of the filter box is fixedly connected with the input port of a hose, and the hose is positioned below the valve.
As a further description of the above technical solution:
the middle part fixedly connected with heat preservation box in base upper end, heat preservation box upper end is provided with the lid, heat preservation box inboard fixedly connected with is controlled relative straight-bar.
As a further description of the above technical solution:
the heat preservation box is characterized in that a container is arranged in the middle of the inner side of the heat preservation box, grooves which are opposite left and right are formed in the outer side wall of the container, and heat preservation lamps which are opposite left and right are arranged on the outer side of the container.
The utility model has the following beneficial effects:
1. according to the utility model, through the mutual operation coordination of the electric push rod, the rack, the gear, the rotating rod, the motor, the rotating shaft, the rotating rod, the stirring plate, the screening layer and the filter box, the effect of stirring, separating and screening stem cells and then filtering and extracting the stem cells is achieved, the problem that the concentration of the stem cells is too high and the direct filtering and extracting efficiency is low is solved, and the extracting efficiency is accelerated.
2. According to the utility model, through the matching of the heat preservation box, the box cover, the container, the straight rod, the groove and the heat preservation lamp, the effect that the exosomes are contained in a constant temperature environment and can be contained in time is achieved, the problem that the exosomes are deactivated due to temperature loss in the exosomes extraction process is solved, and the activity of the exosomes is ensured.
Drawings
FIG. 1 is a front view of a stem cell exosome separation and extraction device according to the present utility model;
FIG. 2 is a side view of a stem cell exosome separation and extraction device according to the present utility model;
FIG. 3 is a schematic diagram of a turnover device of a stem cell exosome separation and extraction device according to the present utility model;
FIG. 4 is a schematic diagram of the inside of a stirred tank of a stem cell exosome separation and extraction device according to the present utility model;
fig. 5 is a schematic diagram of the inside of a thermal insulation box of the stem cell exosome separation and extraction device provided by the utility model.
Legend description:
1. a base; 2. a support column; 3. a platform; 4. a protective sleeve; 5. an electric push rod; 6. a connecting rod; 7. a rack; 8. a gear; 9. a rotating lever; 10. a chute; 11. a slide block; 12. a stirring tank; 13. a rotating shaft; 14. a stirring rod; 15. a stirring plate; 16. a screening layer; 17. a motor; 18. a valve; 19. a filter box; 20. a hose; 21. a thermal insulation box; 22. a box cover; 23. a straight rod; 24. a container; 25. a groove; 26. and a heat preservation lamp.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-5, one embodiment provided by the present utility model is: the utility model provides a stem cell exosome separation extraction element, including base 1, base 1 plays the effect of bearing whole device, base 1 upper end fixedly connected with controls relative support column 2, support column 2 can bear turning device, support column 2 upper end fixedly connected with platform 3, platform 3 upper end fixedly connected with protective sheath 4, protective sheath 4 plays the guard action to turning device, protective sheath 4 front side is provided with electric putter 5, electric putter 5 can stretch out and draw back in vertical direction, electric putter 5 rear end fixedly connected with connecting rod 6, connecting rod 6 carries out the flexible of vertical direction simultaneously along with electric putter 5's drive, connecting rod 6 rear end fixedly connected with rack 7, rack 7 carries out the flexible of vertical direction simultaneously along with the motion of connecting rod 6, rack 7 meshing is connected with gear 8, gear 8 rotates on rack 7, gear 8 left side fixedly connected with dwang 9, dwang 9 rotates along with gear 8's rotation, protective sheath 4 front end inside wall has seted up spout 10, spout 10 sliding connection has slider 11, spout 10 and slider 11 play the effect of playing the spacing to electric putter 5, slider 11 rear end fixedly connected with rack 7 carries out the flexible of vertical direction simultaneously along with the slider 7, rack 7 slides along with the rack 7.
The stirring tank 12 is arranged in the middle of the upper end of the base 1, the stirring tank 12 plays a role in stirring and separating stem cells, a top cover at the upper end of the stirring tank 12 is provided with a feeding port, the top cover can be taken out, the cleaning of the inside is convenient, two discharging ports are arranged at the side end, a front discharging port is positioned below a rear discharging port, the front discharging port flows out of an exosome after stirring and separating, the rear discharging port flows out of the stem cells, a rotating rod 9 is fixedly connected with the outer side of the stirring tank 12 and is left and right opposite, and the stirring tank 12 rotates along with the rotation of the rotating rod 9.
The inside middle part of agitator tank 12 is provided with pivot 13, and pivot 13 can rotate, and relative puddler 14 about pivot 13 outside fixedly connected with, relative puddler 15 about agitator tank 12 inside wall fixedly connected with, puddler 14 and puddler 15 can both stir the separation to stem cell, and pivot 13 below is provided with screening layer 16, and screening layer 16 can screen the separation to the stem cell after stirring.
The upper end of the stirring tank 12 is fixedly connected with a motor 17, the motor 17 is positioned above the base 1, the output end of the motor 17 is fixedly connected with a rotating shaft 13, and the motor 17 can drive the rotating shaft 13 to rotate so as to stir stem cells.
The outside fixedly connected with front and back relative valve 18 of agitator tank 12, valve 18 are located agitator tank 12 front and back end discharge gate department, and front and back end discharge gate is provided with the discharging pipe, and the switch of valve 18 control discharging pipe is closed, and agitator tank 12 front side is provided with filter box 19, is provided with the filter layer in the filter box 19, can filter the exosome that obtains through the separation, obtains the higher exosome of purity.
The lower outlet of the filter box 19 is fixedly connected with the inlet of a hose 20, the hose 20 is positioned below the valve 18, and the hose 20 can be connected to the opening of the box cover 22 of the lower heat preservation box 21 to flow the exosome into the container 24.
The middle part fixedly connected with heat preservation box 21 in base 1 upper end, heat preservation box 21 inside wall and front end are provided with the draw-in groove of controlling relatively, heat preservation box 21 can keep constant temperature to the exosome, heat preservation box 21 upper end is provided with lid 22, lid 22 middle part is made by transparent material, conveniently observe the storage condition, lid 22 front end middle part is provided with the handle, front end both sides fixedly connected with buckle, lid 22 can be through the draw-in groove fixed closing heat preservation box 21 on the heat preservation box 21, be provided with the opening of the same size with hose 20 on the lid 22, lid 22 is convenient for hose 20 insert inside the heat preservation box 21 through the opening, the inboard fixedly connected with of heat preservation box 21 is controlled relative straight-bar 23.
The inside middle part of heat preservation box 21 is provided with receiver 24, and receiver 24 just is to the opening part of lid 22, and receiver 24 can preserve the exosome, and receiver 24 lateral wall is provided with controls relative recess 25, and recess 25 can with straight-bar 23 sliding connection, makes things convenient for the fixed of receiver 24 and takes, and the receiver 24 outside is provided with controls relative heat preservation lamp 26, and heat preservation lamp 26 can carry out thermostatic control to the temperature in the heat preservation box 21, guarantees that the exosome can not lead to inactivation because of losing the temperature.
Working principle: the staff puts into stem cells through the pan feeding mouth on the agitator tank 12 top cap, start motor 17, motor 17 can drive pivot 13 and rotate, the rotation of pivot 13 drives puddler 14 and rotates, stir the separation simultaneously to stem cells with the stirring board 15 of agitator tank 12 inside wall, simultaneously, through the reciprocal flexible of drive electric putter 5, electric putter 5's flexible can drive connecting rod 6 and carry out elevating movement in vertical direction, connecting rod 6 and rack 7 fixed connection, rack 7 carries out reciprocal vertical elevating movement, rack 7's reciprocal elevating movement can drive gear 8 and carry out clockwise and anticlockwise reciprocal rotation, gear 8 and dwang 9 fixed connection, agitator tank 12 and dwang 9 fixed connection, so agitator tank 12 also can carry out clockwise and anticlockwise reciprocal rotation simultaneously, after the stirring is finished, the stem cells are divided into two parts by the screening layer 16, one part is the stem cells, the other part is the exosome, the stem cells flow out from the rear end discharge port by opening the valve 18, the exosome flows into the filter box 19 from the front end discharge port by the screening layer 16, the exosome with higher purity is obtained by filtering again by the filter layer in the filter box 19, the exosome flows into the container 24 to be stored by the opening of the box cover 22 connected to the sealed heat preservation box 21 by the hose 20, the heat preservation lamp 26 is opened to keep the internal environment of the heat preservation box 21 constant temperature, and when the exosome in the container 24 is enough, the container 24 is taken out by the groove 25 for experimental use.
Finally, it should be noted that: the foregoing description is only illustrative of the preferred embodiments of the present utility model, and although the present utility model has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements or changes may be made without departing from the spirit and principles of the present utility model.
Claims (8)
1. The utility model provides a stem cell exosome separation extraction element, includes base (1), its characterized in that: the utility model discloses a base, including base (1), support column (2) upper end fixedly connected with platform (3), platform (3) upper end fixedly connected with protective sheath (4), protective sheath (4) front side is provided with electric putter (5), electric putter (5) rear end fixedly connected with connecting rod (6), connecting rod (6) rear end fixedly connected with rack (7), rack (7) meshing is connected with gear (8), gear (8) left side fixedly connected with dwang (9), spout (10) have been seted up to protective sheath (4) front end inside wall, spout (10) sliding connection slider (11), slider (11) rear end fixedly connected with rack (7).
2. The stem cell exosome separation and extraction device according to claim 1, wherein: the stirring tank (12) is arranged in the middle of the upper end of the base (1), and a rotating rod (9) which is opposite to each other left and right is fixedly connected to the outer side of the stirring tank (12).
3. The stem cell exosome separation and extraction device according to claim 2, wherein: the stirring tank is characterized in that a rotating shaft (13) is arranged in the middle of the inner side of the stirring tank (12), a stirring rod (14) opposite to each other is fixedly connected to the outer side of the rotating shaft (13), a stirring plate (15) opposite to each other is fixedly connected to the inner side wall of the stirring tank (12), and a screening layer (16) is arranged below the rotating shaft (13).
4. The stem cell exosome separation and extraction device according to claim 2, wherein: the stirring tank (12) is fixedly connected with a motor (17) at the upper end, and the motor (17) is positioned above the base (1).
5. The stem cell exosome separation and extraction device according to claim 2, wherein: the outside of agitator tank (12) fixedly connected with front and back relative valve (18), agitator tank (12) front side is provided with filter cartridge (19).
6. The stem cell exosome separation and extraction device according to claim 5, wherein: the output port at the lower end of the filter box (19) is fixedly connected with the input port of a hose (20), and the hose (20) is positioned below the valve (18).
7. The stem cell exosome separation and extraction device according to claim 1, wherein: the novel heat preservation box is characterized in that the middle part of the upper end of the base (1) is fixedly connected with a heat preservation box (21), the upper end of the heat preservation box (21) is provided with a box cover (22), and the inner side of the heat preservation box (21) is fixedly connected with a straight rod (23) which is opposite left and right.
8. The stem cell exosome separation and extraction device according to claim 7, wherein: the heat preservation box is characterized in that a container (24) is arranged in the middle of the inner side of the heat preservation box (21), grooves (25) opposite to each other in the left and right are formed in the outer side wall of the container (24), and heat preservation lamps (26) opposite to each other in the left and right are arranged on the outer side of the container (24).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322058327.1U CN220564583U (en) | 2023-08-02 | 2023-08-02 | Stem cell exosome separation and extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322058327.1U CN220564583U (en) | 2023-08-02 | 2023-08-02 | Stem cell exosome separation and extraction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220564583U true CN220564583U (en) | 2024-03-08 |
Family
ID=90102058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322058327.1U Active CN220564583U (en) | 2023-08-02 | 2023-08-02 | Stem cell exosome separation and extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220564583U (en) |
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2023
- 2023-08-02 CN CN202322058327.1U patent/CN220564583U/en active Active
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