CN220503016U - Influenza virus isothermal amplification nucleic acid detection kit - Google Patents
Influenza virus isothermal amplification nucleic acid detection kit Download PDFInfo
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- CN220503016U CN220503016U CN202322004062.7U CN202322004062U CN220503016U CN 220503016 U CN220503016 U CN 220503016U CN 202322004062 U CN202322004062 U CN 202322004062U CN 220503016 U CN220503016 U CN 220503016U
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- 241000712461 unidentified influenza virus Species 0.000 title claims abstract description 29
- 238000001514 detection method Methods 0.000 title claims abstract description 26
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 23
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 23
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 23
- 238000011901 isothermal amplification Methods 0.000 title claims abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 74
- 238000005070 sampling Methods 0.000 claims abstract description 33
- 239000003814 drug Substances 0.000 claims abstract description 15
- 229940079593 drug Drugs 0.000 claims abstract description 10
- 238000009434 installation Methods 0.000 claims abstract description 8
- 230000008878 coupling Effects 0.000 claims abstract description 5
- 238000010168 coupling process Methods 0.000 claims abstract description 5
- 238000005859 coupling reaction Methods 0.000 claims abstract description 5
- 238000005192 partition Methods 0.000 claims description 36
- 238000007789 sealing Methods 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 241000700605 Viruses Species 0.000 abstract description 6
- 210000002345 respiratory system Anatomy 0.000 abstract description 4
- 206010022000 influenza Diseases 0.000 description 5
- 241000712431 Influenza A virus Species 0.000 description 4
- 241000713196 Influenza B virus Species 0.000 description 4
- 101710154606 Hemagglutinin Proteins 0.000 description 3
- 102000005348 Neuraminidase Human genes 0.000 description 3
- 108010006232 Neuraminidase Proteins 0.000 description 3
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 3
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 3
- 101710176177 Protein A56 Proteins 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 239000000185 hemagglutinin Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 241000713297 Influenza C virus Species 0.000 description 2
- 102000011931 Nucleoproteins Human genes 0.000 description 2
- 108010061100 Nucleoproteins Proteins 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001932 seasonal effect Effects 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000712464 Orthomyxoviridae Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 244000309457 enveloped RNA virus Species 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000017188 evasion or tolerance of host immune response Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 238000010185 immunofluorescence analysis Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000012755 real-time RT-PCR analysis Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Abstract
The application relates to an influenza virus isothermal amplification nucleic acid detection kit relates to the technical field of medicine containers, including box body and lid, can dismantle through coupling assembling in the box body and be connected with the baffle, placed the drug sensitive board in the box body, have a plurality of reagent pipes through multiunit installation component demountable installation on the box body, can dismantle through assembly component on the lid and be connected with sampling rod and burette. The application is through opening the lid, take off sampling stick and burette from the lid, use the sampling stick to sample human respiratory tract, then unblock coupling assembling takes out baffle and medicine sensitive plate from the box body, unblock installation component takes off each reagent pipe that is equipped with different reagents from the baffle, use the burette to drip into the medicine sensitive plate with the intraductal reagent of each reagent, then use the sampling stick to detect the sample, thereby the self-checking work of influenza virus has been accomplished, earlier, the more accurate detection of influenza virus has been realized with lower cost, thereby the virus propagation has been blocked to effectual quick.
Description
Technical Field
The application relates to the technical field of medical containers, in particular to an influenza virus isothermal amplification nucleic acid detection kit.
Background
Seasonal influenza viruses are the major respiratory pathogens responsible for annual epidemics, pose a great threat to human health, and World Health Organization (WHO) surveys indicate that 5% -10% of the population infects worldwide each year, resulting in 300-500 thousands of severe disease cases and 29-65 thousands of deaths. Influenza virus belongs to orthomyxoviridae, is an enveloped RNA virus, has a spherical appearance with a diameter of 80-100 nm or a filiform with a length of thousands of nanometers, and consists of an envelope and a nucleocapsid. Influenza viruses can be classified into A, B, C and D, and recent studies have shown that viruses capable of infecting humans are classified into three types according to their matrix (M) or Nucleoprotein (NP) genes: influenza A Virus (IAV), influenza B Virus (IBV), and Influenza C Virus (ICV); among them IAV and IBV can cause seasonal influenza epidemics. Whereas IAVs can be further divided into several subtypes based on the genes of the influenza envelope components glycoprotein Hemagglutinin (HA) and Neuraminidase (NA). IAV, unlike other types, can spread between animals and humans and, due to changes in HA and NA antigen structure, can undergo antigen transfer and drift, effectively achieving immune escape, resulting in annual epidemics worldwide, as well as influenza pandemics. IBV does not undergo antigen transfer and therefore does not cause pandemic infection. Therefore, rapid and accurate diagnosis of influenza infection at an early stage is of great importance for blocking transmission.
Several methods have been developed and are commonly used in clinical practice such as immunofluorescence analysis, immunochromatography analysis, enzyme-linked immunoassay (ELISA) and real-time RT-PCR analysis. In clinical practice, conventional real-time RT-PCR is the primary method for detecting and diagnosing influenza. However, the high cost and long time limit its use in an outpatient clinical setting.
Disclosure of Invention
In order to improve the convenience and universality of influenza virus nucleic acid detection, the application provides an influenza virus isothermal amplification nucleic acid detection kit.
The application provides an influenza virus isothermal amplification nucleic acid detection kit, adopts following technical scheme:
the utility model provides an influenza virus isothermal amplification nucleic acid detection kit, includes the box body and rotates the lid that connects blocking to the box body upper shed on the box body, can dismantle through coupling assembling in the box body and be connected with the baffle, the baffle is the horizontality, the baffle divides into upper space and lower space two parts with the box body inside, the drug sensitive board has been placed in the lower space of box body, a plurality of reagent pipes have been installed through multiunit installation component demountable installation in the upper space of box body for sample collection and the processing reagent of nucleic acid detection, nucleic acid extraction reagent, nucleic acid amplification reagent, detection reagent, positive control and negative control are divided into respectively in each reagent pipe, can dismantle through assembly subassembly and be connected with sampling rod and burette on the lid.
Through adopting above-mentioned technical scheme, open the lid, unblock assembly subassembly takes off sampling stick and burette from the lid, use sampling stick to sample human respiratory tract, then unblock coupling assembling takes out the baffle from the box body, take out the medicine sensitive plate of placing in the baffle below, unblock installation component takes off each reagent pipe that is equipped with different reagents from the baffle, use the burette to drip into the medicine sensitive plate with the intraductal reagent of each reagent, then use sampling stick to detect the sample, thereby the self-checking work of influenza virus has been accomplished, and is convenient and fast, easy operation has realized earlier, the more accurate detection of influenza virus with lower cost, thereby effectively quick the viral propagation of having blocked.
Optionally, the connection assembly includes:
the four bearing tables are arranged on the inner side wall of the box body and are respectively positioned at four corners of the inner side wall of the box body, and the four bearing tables support the four corners of the partition plate;
the connecting column, vertical connecting hole has been seted up to the baffle bottom, the connecting column sets up on the plummer and is vertical state, the connecting column is pegged graft with the connecting hole and is cooperated.
By adopting the technical scheme, the connecting holes at the four corners of the partition board are aligned with the positions of the connecting columns in the vertical direction by moving the partition board, then the partition board is put down to enable the connecting columns to penetrate into the connecting holes, and the bearing table supports the partition board, so that the fixing work of the partition board in the box body is completed; when the partition plate is required to be taken out, the partition plate is lifted upwards, so that the connecting holes are separated from the connecting columns, and the device is convenient and quick and has high working efficiency.
Optionally, a circular arc-shaped guiding surface is formed at the top end of the connecting column.
Through adopting above-mentioned technical scheme, set up convex guide surface on the top of spliced pole to when the spliced pole penetrates in the connecting hole, the edge contact of guide surface and connecting hole on the baffle can lead the baffle position, thereby makes the spliced pole insert smoothly in the connecting hole, has improved spliced pole and spliced hole and has carried out grafting efficiency of grafting.
Optionally, the mounting assembly includes:
the two limiting plates are arranged on the partition plate and are in a vertical state, and the two limiting plates respectively abut against two opposite outer side walls of the reagent tube;
the two limiting rods are arranged, are both rotatably arranged on the partition plate and are respectively positioned at two sides of the reagent tube, which are not contacted with the limiting plate;
the driving piece is arranged on the partition plate and used for driving the two limiting rods to rotate so as to clamp and fix the reagent tube.
Through adopting above-mentioned technical scheme, remove reagent pipe and place on the baffle, two limiting plates carry out the butt to two lateral walls that are on the back of the body mutually of reagent pipe, then two gag lever posts clamp the remaining both sides wall that are on the back of the body mutually of reagent pipe under the driving piece effect, and two gag lever posts and two limiting plate cooperation are fixed reagent pipe position to the fixed work of reagent pipe position on the baffle has been accomplished.
Optionally, the driving member includes:
the driving rods are rotatably arranged on the partition plates, the rotating points of the driving rods are positioned at the top ends of the driving rods, two driving rods are arranged, and two limiting rods are respectively arranged at the top ends of the two driving rods, and the length direction of each limiting rod is mutually perpendicular to the length direction of each driving rod;
the two ends of the driving spring are respectively connected with the bottom ends of the two driving rods, and when the driving spring is in a natural state, the two limiting rods are mutually abutted together.
Through adopting above-mentioned technical scheme, the bottom compression drive spring that rotates two actuating levers and makes two actuating levers, two actuating levers remove and drive and be located two actuating levers top gag lever posts and keep away from each other to make between two gag lever posts produce the space that supplies the reagent pipe to place, put into the reagent pipe in the space, then loosen two actuating levers, two actuating levers rotate under the effect of drive spring recovery deformation force and drive two gag lever levers and support tightly on the reagent pipe lateral wall, thereby accomplished the removal of two gag lever levers and to the centre gripping work of reagent pipe.
Optionally, the assembly component includes:
a fitting pipe provided on the cover body;
the sealing cover is arranged on the assembly pipe, a moving hole is formed in the sealing cover, and the head of the sampling rod or the dropper penetrates through the moving hole and stretches into the assembly pipe;
the fixing piece is arranged on the sealing cover and used for fixing the sampling rod or the dropper.
Through adopting above-mentioned technical scheme, remove sampling rod or burette and make the head of sampling rod or burette pass the removal hole on the sealing cap and stretch into the assembly intraductal, then use the mounting to fix the position of sampling rod or burette on the removal hole to accomplish the fixed work of sampling rod and burette on the lid, both rationally applied the space on the box lid, also reduced simultaneously sampling rod and burette head receive the probability of external environmental pollution, improved the accuracy of detection.
Optionally, the fixing member includes:
the fixed rod is arranged on the sealing cover in a sliding way, one end of the fixed rod is positioned outside the sealing cover, and the other end of the fixed rod extends into the moving hole;
the fixed spring is arranged on the sealing cover and connected with the fixed rod, and the fixed rod is abutted against the sampling rod or the dropper under the action of the fixed spring.
Through adopting above-mentioned technical scheme, remove dead lever compression fixed spring and remove to make the dead lever remove from the removal hole, be convenient for sample stick or burette insert in the assembly pipe, sample stick or burette insert the back of accomplishing, loosen the dead lever, the dead lever supports tightly on sample stick or burette lateral wall under the fixed spring effect, thereby with sample stick or burette spacing in the removal hole.
Optionally, a rubber soft sleeve is sleeved on the end part of one end of the limiting rod, which is contacted with the reagent tube.
Through adopting above-mentioned technical scheme, the soft cover of rubber is established to the cover on the terminal surface of gag lever post and reagent pipe contact, and the soft cover of rubber buffers when being contacted gag lever post and reagent pipe lateral wall, has reduced the probability that gag lever post and reagent pipe lateral wall make reagent pipe damage because of rigid contact, and has increased the frictional force between gag lever post and the reagent pipe lateral wall simultaneously, has improved the fixed effect of gag lever post to the reagent pipe.
In summary, the present application includes at least one of the following beneficial technical effects:
1. the box cover is opened, the sampling rod and the dropper are taken down from the box cover by the unlocking assembly component, the sampling rod is used for sampling the respiratory tract of a human body, then the partition plate is taken out from the box body by the unlocking connection component, the drug sensitive plate placed below the partition plate is taken out, the reagent tubes filled with different reagents are taken down from the partition plate by the unlocking assembly component, the reagents in the reagent tubes are dripped into the drug sensitive plate by the dropper, and then the sample is detected by the sampling rod, so that the self-checking work of influenza viruses is completed, the operation is convenient and quick, the operation is simple, the earlier and more accurate detection of the influenza viruses is realized at lower cost, and the virus transmission is effectively and rapidly blocked;
2. the arc-shaped guide surface is arranged at the top end of the connecting column, so that when the connecting column penetrates into the connecting hole, the guide surface is contacted with the edge of the connecting hole on the partition plate to guide the position of the partition plate, the connecting column is smoothly inserted into the connecting hole, and the plugging efficiency of plugging the connecting column with the plugging hole is improved;
3. through the soft cover of rubber is established to the cover on the terminal surface of gag lever post and reagent pipe contact, cushion when the gag lever post is contacted with the reagent pipe lateral wall, reduced the probability that gag lever post and reagent pipe lateral wall make the reagent pipe damage because of rigid contact, and increased the frictional force between gag lever post and the reagent pipe lateral wall simultaneously, improved the fixed effect of gag lever post to the reagent pipe.
Drawings
FIG. 1 is a schematic perspective view of the present application;
FIG. 2 is a schematic cross-sectional view of A-A of FIG. 1;
FIG. 3 is a schematic view of the mounting assembly of the present application with the bulkhead sidewall sectioned.
Reference numerals: 1. a case body; 11. a box cover; 12. a partition plate; 13. a drug sensitive plate; 14. a sampling rod; 15. a dropper; 16. a reagent tube; 2. a connection assembly; 21. a carrying platform; 22. a connecting column; 23. a guide surface; 3. a mounting assembly; 31. a limiting plate; 32. a limit rod; 33. a driving member; 34. a driving rod; 35. a drive spring; 4. assembling the assembly; 41. assembling a pipe; 42. sealing cover; 43. a fixing member; 44. a fixed rod; 45. and fixing the spring.
Detailed Description
The present application is described in further detail below in conjunction with fig. 1-3.
The embodiment of the application discloses an influenza virus isothermal amplification nucleic acid detection kit.
Referring to fig. 1 and 2, the influenza virus isothermal amplification nucleic acid detection kit comprises a box body 1 and a box cover 11 rotatably connected to the box body 1 to block an opening in the box body 1, wherein a horizontal partition plate 12 is detachably connected to the box body 1 through a connecting component 2, the partition plate 12 divides the interior of the box body 1 into an upper space and a lower space, and a drug sensitive plate 13 is arranged in the lower space of the box body 1. A plurality of reagent tubes 16 are detachably arranged in the upper space of the box body 1 through a plurality of groups of mounting assemblies 3, and sample collection and treatment reagents, nucleic acid extraction reagents, nucleic acid amplification reagents, detection reagents, positive control and negative control for nucleic acid detection are respectively packaged in the reagent tubes 16, and a sampling rod 14 and a dropper 15 are detachably connected to the box cover 11 through an assembly 4.
Referring to fig. 2, the connecting assembly 2 includes four bearing tables 21 and connecting columns 22, the bearing tables 21 are all fixedly connected to the inner side wall of the box body 1, and the four bearing tables 21 are respectively located at four corners of the inner side wall of the box body 1. The partition plate 12 is placed on the upper surfaces of four carrying tables 21, and the four carrying tables 21 cooperate to support the partition plate 12. The connecting column 22 is fixedly connected to the upper surface of the carrying table 21 and is in a vertical state. The partition plate 12 is provided with a connecting hole vertically penetrating through the partition plate 12. The connecting posts 22 are in plug-in fit with the connecting holes. The top end of the connecting column 22 is provided with a circular arc-shaped guide surface 23.
Referring to fig. 2 and 3, the reagent vessel 16 is mounted on the partition plate 12 by a mounting assembly 3, and the mounting assembly 3 includes a limiting plate 31, a limiting rod 32, and a driving member 33. The limiting plate 31 is provided with two pieces. The two limiting plates 31 are fixedly connected to the upper surface of the partition plate 12 and are in a vertical state. The reagent tube 16 is placed between two limiting plates 31, and the two limiting plates 31 are abutted against two opposite side walls of the reagent tube 16. The stopper rod 32 is rotatably provided to the separator 12 by a driving member 33, and clamps the opposite side walls of the reagent tube 16 which are not in contact with the stopper plate 31.
Referring to fig. 3, the driving member 33 includes a driving lever 34 and a driving spring 35. The driving rod 34 is rotatably connected to the partition 12, and a mounting cavity is formed in the partition 12. The point of rotation of the drive rod 34 is located at the top end of the drive rod 34. The drive rod 34 is located entirely within the mounting cavity. The drive rods 34 are provided in two, and the two drive rods 34 are respectively positioned on both sides of the reagent vessel 16 which are not in contact with the limiting plate 31. The limiting rod 32 is fixedly connected to the top end of the driving rod 34 and axially perpendicular to the driving rod 34. The drive spring 35 is located in the mounting cavity and has two ends connected to the bottom ends of the two drive rods 34, respectively. When the drive spring 35 is in a natural state, the two limit rods 32 are abutted against each other. A rubber soft sleeve is sleeved on one end of the two limiting rods 32, which is contacted with the reagent tube 16.
Referring to fig. 3, the two driving rods 34 are rotated to compress the driving springs 35 at the bottom ends of the two driving rods 34, the two driving rods 34 move to drive the limiting rods 32 above the two driving rods 34 to move away from each other, so that a gap for placing the reagent tube 16 is formed between the two limiting rods 32, the reagent tube 16 is placed in the gap, then the two driving rods 34 are loosened, the two driving rods 34 rotate under the action of restoring deformation force of the driving springs 35 and drive the two limiting rods 32 to abut against the outer side wall of the reagent tube 16, and therefore the movement of the two limiting rods 32 and the clamping work of the reagent tube 16 are completed.
Referring to fig. 2, either the sampling wand 14 or the drop tube 15 is removably mounted to the cap by a mounting assembly 4, the mounting assembly 4 including a mounting tube 41, a sealing cap 42 and a securing member 43. The fitting tube 41 is fixedly connected to the lower surface of the cover body in a horizontal state, one end of the fitting tube 41 is in an open state, and the sealing cover 42 is fixedly connected to the open end of the fitting tube 41. The seal cover 42 is provided with a moving hole in a horizontal direction. The sampling wand 14 or the drip tube 15 extends the head into the mounting tube 41 through the displacement bore. The fixing member 43 is used for fixing the position of the sampling rod 14 or the dropper 15 on the movement hole.
Referring to fig. 2, the fixing member 43 includes a fixing lever 44 and a fixing spring 45. The fixing rod 44 is vertically slidably mounted on the sealing cover 42. The top end of the fixed rod 44 extends into the moving hole, and the bottom end of the fixed rod 44 is positioned outside the sealing cover 42. The fixed spring 45 is sleeved on the fixed rod 44, one end of the fixed spring is connected with the lower surface of the sealing cover 42, and the other end of the fixed spring is fixedly connected with the fixed rod 44. The top end of the fixing rod 44 abuts against the side wall of the sampling rod 14 or the dropper 15 under the action of the fixing spring 45.
The working principle of the embodiment of the application is as follows:
the cover is opened, the partition 12 is moved to remove the partition 12 from the box 1, and the drug sensitive plate 13 is taken out from the bottom of the box 1. The fixing rod 44 is pulled, the sampling rod 14 and the dropper 15 are taken out from the assembly tube 41 of the box cover 11, then the various reagent tubes 16 are sequentially taken out from the partition plate 12, various reagents are dripped onto the drug sensitive plate 13 by using the dropper 15, finally the sampling rod 14 is used for sampling viruses of the respiratory tract of a human body and then immersing the viruses into the reagents on the drug sensitive plate 13 for detection, so that the self-checking work of influenza viruses is completed, the operation is convenient and quick, the operation is simple, the earlier and more accurate detection of the influenza viruses is realized with lower cost, and the virus transmission is effectively and quickly blocked.
The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.
Claims (8)
1. An influenza virus isothermal amplification nucleic acid detection kit is characterized in that: including box body (1) and rotate connect box body (1) upper shed block lid (11), can dismantle through coupling assembling (2) in box body (1) and be connected with baffle (12), baffle (12) are the horizontality, baffle (12) divide into upper space and lower space two parts with box body (1) inside, place drug sensitive board (13) in the lower space of box body (1), install a plurality of reagent pipes (16) through multiunit installation component (3) demountable installation in the upper space of box body (1), be used for sample collection and processing reagent, nucleic acid extraction reagent, nucleic acid amplification reagent, detection reagent, positive contrast and negative contrast respectively the partial shipment in each in reagent pipe (16), can dismantle through assembly assembling subassembly (4) on lid (11) and be connected with sampling rod (14) and burette (15).
2. The influenza virus isothermal amplification nucleic acid detection kit according to claim 1, wherein: the connection assembly (2) comprises:
the bearing tables (21) are arranged, the four bearing tables (21) are arranged on the inner side wall of the box body (1) and are respectively positioned at four corners of the inner side wall of the box body (1), and the four bearing tables (21) support the four corners of the partition board (12);
the connecting column (22), vertical connecting hole has been seted up to baffle (12) bottom, connecting column (22) set up on plummer (21) and be vertical state, connecting column (22) are pegged graft with the connecting hole and are cooperated.
3. The influenza virus isothermal amplification nucleic acid detection kit according to claim 2, wherein: the top end of the connecting column (22) is provided with a circular arc-shaped guide surface (23).
4. The influenza virus isothermal amplification nucleic acid detection kit according to claim 1, wherein: the mounting assembly (3) comprises:
the two limiting plates (31) are arranged, the two limiting plates (31) are arranged on the partition plate (12) and are in a vertical state, and the two limiting plates (31) are respectively abutted against two opposite outer side walls of the reagent tube (16);
the two limiting rods (32) are arranged, and the two limiting rods (32) are both rotatably arranged on the partition plate (12) and are respectively positioned at two sides of the reagent tube (16) which are not contacted with the limiting plate (31);
the driving piece (33), the driving piece (33) is arranged on the partition board (12) and is used for driving the two limiting rods (32) to rotate so as to clamp and fix the reagent tube (16).
5. The kit for isothermal amplification of influenza virus according to claim 4, wherein: the driving member (33) includes:
the driving rods (34) are rotatably arranged on the partition plates (12), the rotating points of the driving rods (34) are positioned at the top ends of the driving rods (34), two driving rods (34) are arranged, two limiting rods (32) are respectively arranged at the top ends of the two driving rods (34), and the length direction of the limiting rods (32) is mutually perpendicular to the length direction of the driving rods (34);
the two ends of the driving spring (35) are respectively connected with the bottom ends of the two driving rods (34), and when the driving spring (35) is in a natural state, the two limiting rods (32) are mutually abutted together.
6. The influenza virus isothermal amplification nucleic acid detection kit according to claim 1, wherein: the fitting assembly (4) comprises:
a fitting tube (41), the fitting tube (41) being provided on the cover;
the sealing cover (42) is arranged on the assembly pipe (41), a moving hole is formed in the sealing cover (42), and the head of the sampling rod (14) or the dropper (15) penetrates through the moving hole and stretches into the assembly pipe (41);
the fixing piece (43) is arranged on the sealing cover (42) and used for fixing the sampling rod (14) or the dropper (15).
7. The kit for isothermal amplification of influenza virus according to claim 6, wherein: the fixing member (43) includes:
the fixing rod (44) is arranged on the sealing cover (42) in a sliding manner, one end of the fixing rod (44) is positioned outside the sealing cover (42), and the other end of the fixing rod (44) extends into the moving hole;
the fixed spring (45), fixed spring (45) set up on sealed lid (42) and be connected with dead lever (44), dead lever (44) support tightly on sampling stick (14) or burette (15) under fixed spring (45) effect.
8. The kit for isothermal amplification of influenza virus according to claim 5, wherein: a rubber soft sleeve is sleeved on the end part of one end of the limiting rod (32) contacted with the reagent tube (16).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202322004062.7U CN220503016U (en) | 2023-07-27 | 2023-07-27 | Influenza virus isothermal amplification nucleic acid detection kit |
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Application Number | Priority Date | Filing Date | Title |
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CN202322004062.7U CN220503016U (en) | 2023-07-27 | 2023-07-27 | Influenza virus isothermal amplification nucleic acid detection kit |
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Publication Number | Publication Date |
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CN220503016U true CN220503016U (en) | 2024-02-20 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202322004062.7U Active CN220503016U (en) | 2023-07-27 | 2023-07-27 | Influenza virus isothermal amplification nucleic acid detection kit |
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2023
- 2023-07-27 CN CN202322004062.7U patent/CN220503016U/en active Active
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