CN2204806Y

CN2204806Y - Spot enzyme labelling reagent diagnosis box

Info

Publication number: CN2204806Y
Application number: CN 94229827
Authority: CN
Inventors: 曾明安; 屈振麒; 黄杰; 李娟佑; 徐亮
Original assignee: SICHUAN INST OF PREVENTION AND TREATMENT OF PARASITIC DISEASES
Current assignee: SICHUAN INST OF PREVENTION AND TREATMENT OF PARASITIC DISEASES
Priority date: 1994-11-08
Filing date: 1994-11-08
Publication date: 1995-08-09
Anticipated expiration: 2004-11-08

Abstract

The utility model relates to a spot enzyme sample reagent diagnosing box, belonging to a carrier for the testing diagnosis of the spot enzyme samples of the diseases such as parasite, etc. in preventive medicine, medicine, and veterinary medicine. The utility model is a reagent diagnosing box made of white PVC, the reagent diagnosing box has a smooth surface and a convex testing hole whose bottom has the corresponding antigens or antibodies of the tested diseases, and the surface of the reagent diagnosing box is closed. The utility model is suitable for clinical rapid diagnosis and the rapid testing of large considerable quantity of samples in sites, and is especially suitable for the diagnosis and the testing of the spot or the liquid enzyme samples of clonorchiasis sinensis, bilharziasis, paragonimiasis, echinococcosis, cysticercosis, and filariasis. The utility model has the advantages of high sensitivity, high specificity, conventional operation, convenient operation, accurate results, and easy preservation, and the utility model is beneficial to the application to establishment units.

Description

Spot enzyme labelling reagent diagnosis box

The utility model relates to the carrier of preventive medicine, clinical medicine and veterinary science spot enzyme mark (Dot-ELISA) test diagnosis, especially for the test diagnosis carrier to diseases such as parasites.

At present, at home and abroad medical science and veterinary field are marked diseases such as test diagnosis parasite with the spot enzyme, all adopt the nitrocellulose membrane (NC) or the blend fiber film (MC) of white to make test carrier, its testing sequence is: 1. put the corresponding antigens that the follow-up disease is arranged on above-mentioned tunica fibrosa, be adsorbed to dried; 2. it is cut and be equipped with in the machine glass shrinkage pool plate, add the serum to be checked of dilution, incubation 1.5～2 hours, washing; 3. in detecting shrinkage pool, add enzyme conjugates, as preceding incubation, washing; 4. add substrate colour developing, washing, sentence read result.Its weak point has: need to be equipped with in addition other test equipment 1.; 2. Chang Yong poly (methyl methacrylate) plate shrinkage pool is made test chamber, and is thicker at the bottom of its hole, and it is bad to conduct heat, and the test speed of response is slower, needs 1.5～2 hours usually; 3. tunica fibrosa easily is washed liquid and mixes towards turn-over or with the adjacent holes diaphragm when washing, easily loses because of carelessness when inclining washings; 4. the reagent consumption is more; 5. the colour developing background influences result's judgement more deeply; 6. for future reference as preserving sample, it is dried to need taking-up one by one to put, and is affixed on minute book; 7. as using the masses of fibres film test, though the fraud of avoiding diaphragm to lose, the point sample spacing is wide, and wants more reagent flood diaphragm.In sum, use prior art, waste time and energy, take cost, diagnostic result has error, inconvenient saving result, and unfavorable grass-roots unit big area is looked into disease and is used.

The purpose of this utility model be to overcome above-mentioned weak point of the prior art and provide a kind of susceptibility height, high specificity, working specification, easy to use, result accurately, be easy to preserve, be beneficial to the spot enzyme marking reagent diagnosis box of popularization.

The design's structure is to be made into smooth surface and with the reagent diagnosis box that detects shrinkage pool more than two, its bottom of detecting shrinkage pool is differential of the arc shape by white igelite.Diagnosis box can be made 10 and detect shrinkage pool, divide and classify two row's plate faces as, four jiaos of the plate face is the garden arc, and detecting in the shrinkage pool bottom has corresponding antigens or the antibody that is adsorbed to dried disease to be measured, and the box face has sealing membrane.Testing sequence is summarized as follows:

1. open the sealing membrane of above-mentioned diagnosis box, increase serum diluent and serum mixing to be checked in the hole, incubation 15 minutes, washing; 2. in detecting shrinkage pool, add enzyme conjugates, incubation, washing; 3. add the substrate colour developing, the flushing sentence read result.

The utility model compared with prior art has following advantage:

1. do not need to be equipped with in addition the equipment of test chamber and dilute serum, easy and simple to handle;

2. serum dilution and serum to be checked once add in the detection shrinkage pool, have reduced the application of sample error;

3. the design's good heat-transfer speed of response is fast, and the test whole process only needs 30 minutes, can report the result;

4. save reagent 4/5 than prior art, reduce cost effectively;

5. can not need the foreign protein blocking-up with the design, the susceptibility height, background white, high specificity, clear spot can distinguish that the result is accurate;

6. test-results is preserved conveniently, only needs get final product in the dark place lucifuge;

7. the design also is applicable to the enzyme mark test of liquid phase.

In a word, the design is applicable to the rapid detection of preventive medicine, medical science, veterinary clinical quick diagnosis and on-the-spot big quantity sample, and practicality is widely arranged.Successfully try out in the spot enzyme mark diagnostic detection of clonorchiasis sinensis, schistosomicide, pulmonary distomiasis, hydatidosis, cysticercosis and filaricide and the liquid phase enzyme mark diagnostic detection of clonorchiasis sinensis.

The design's accompanying drawing drawing is illustrated as: Fig. 1 is a front view of the present utility model, and Fig. 2 is a vertical view of the present utility model, and Fig. 3 is a shaft side figure of the present utility model.

The utility model is described in further detail below with reference to embodiment and accompanying drawing:

1. 1 kinds of spot enzyme marking reagents of embodiment diagnosis box, by smooth surface and with 2 white polyvinyl chloride rectangle reagent diagnosis boxes that detect shrinkage pool ［ 1 ］, its bottom of detecting shrinkage pool is differential of the arc shape, four jiaos of the plate face is the garden arc, detecting in the shrinkage pool bottom has corresponding antigens or the antibody that is adsorbed to dried disease to be measured, and the box face has sealing membrane ［ 2 ］.

2. 1 kinds of spot enzyme marking reagents of embodiment diagnosis box, by smooth surface and be with 10 to detect shrinkage pools ［ 1 ］, divide the white polyvinyl chloride rectangle reagent diagnosis box of classifying * 5 of two rows as, its bottom of detecting shrinkage pool is differential of the arc shape, four jiaos of the plate face is the lonely shape in garden, detecting in the shrinkage pool bottom has corresponding antigens or the antibody that is adsorbed to dried disease to be measured, and the box face has sealing membrane ［ 2 ］.

3. 1 kinds of spot enzyme marking reagents of embodiment diagnosis box, by smooth surface and be with 50 to detect shrinkage pools ［ 1 ］, divide the white polyvinyl chloride rectangle reagent diagnosis box of classifying * 10 of 5 rows as, its bottom of detecting shrinkage pool is differential of the arc shape, four jiaos of the plate face is the garden arc, detecting in the shrinkage pool bottom has corresponding antigens or the antibody that is adsorbed to dried disease to be measured, and the box face has sealing membrane ［ 2 ］.

Claims

1, a kind of spot enzyme marking reagent diagnosis box, it is characterized in that: by smooth surface and with the white polyvinyl chloride reagent diagnosis box that detects shrinkage pool [1] more than two, its bottom of detecting shrinkage pool is differential of the arc shape, detecting in the shrinkage pool bottom has corresponding antigens or the antibody that is adsorbed to dried disease to be measured, and the box face has sealing membrane [2].

2, spot enzyme marking reagent diagnosis box according to claim 1, it is characterized in that: by smooth surface and be with 10 to detect shrinkage pools ［ 1 ］, divide the white polyvinyl chloride rectangle reagent diagnosis box ［ 2 ］ of classifying * 5 of two rows as, its bottom of detecting shrinkage pool is differential of the arc shape, four jiaos of the plate face is the garden arc, detecting in the shrinkage pool bottom has corresponding antigens or the antibody that is adsorbed to dried disease to be measured, and the box face has sealing membrane ［ 2 ］.