CN220376681U - Device for detecting heat resistance of probiotics group - Google Patents
Device for detecting heat resistance of probiotics group Download PDFInfo
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- CN220376681U CN220376681U CN202321831732.6U CN202321831732U CN220376681U CN 220376681 U CN220376681 U CN 220376681U CN 202321831732 U CN202321831732 U CN 202321831732U CN 220376681 U CN220376681 U CN 220376681U
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- operation room
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- 239000006041 probiotic Substances 0.000 title claims abstract description 43
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 43
- 230000007246 mechanism Effects 0.000 claims abstract description 59
- 238000001514 detection method Methods 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 230000000529 probiotic effect Effects 0.000 claims abstract description 17
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 6
- 238000001125 extrusion Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000001816 cooling Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000012865 aseptic processing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a device for detecting heat resistance of probiotics, which comprises a main body, wherein an aseptic operation chamber is arranged in the main body, a pipetting mechanism is arranged above the aseptic operation chamber, a feeding mechanism is arranged on one side of the main body, a first constant-temperature water bath box and a second constant-temperature water bath box are arranged on one side of the feeding mechanism, a culture tank is arranged in the aseptic operation chamber, a control console is arranged on the outer side of the main body, and a display screen is arranged on the control console. The utility model provides a device for detecting the heat resistance of a probiotic colony, which can automatically detect the heat resistance of the probiotic colony, is always in a sterile environment and has more accurate detection; and the sterile operation room can be automatically sterilized after operation, so that the next detection is facilitated.
Description
Technical Field
The utility model belongs to the technical field of detection of probiotics, and particularly relates to a device for detecting heat resistance of probiotics.
Background
Probiotics are a class of active microorganisms beneficial to a host by colonizing the human body and altering the flora composition of a part of the host. The probiotics keep activity in a certain temperature range, and the probiotics are killed when the temperature is too high. In order to ensure the activity of the probiotics, the heat resistance of the probiotics needs to be detected.
In the prior art, as disclosed in Chinese patent publication No. CN 210856071U, a detection device for culturing probiotics can be used for carrying out multi-layer sampling in an incubator, then detecting samples each time, and knowing the condition of each layer of probiotics in the incubator, so that the error generated by the detection result is minimized, and meanwhile, due to the arc shapes arranged on the two sides of the sampling block, no redundant probiotics exist in the sampling tube, so that the probiotics are reduced. But the heat resistance of probiotics cannot be detected, and the detection effect is easily affected due to the lack of a sterile environment.
Disclosure of Invention
The utility model aims to provide a device for detecting heat resistance of probiotics, which solves the problems that the heat resistance of probiotics cannot be detected and a sterile environment is lacking and the detection effect is easily affected in the prior art.
To this end, the utility model provides a device for the detection of heat resistance of a probiotic group, comprising: the automatic incubator comprises a main body, wherein an aseptic operation room is arranged inside the main body, a pipetting mechanism is arranged above the aseptic operation room, a feeding mechanism is arranged on one side of the main body, a first constant-temperature water bath box and a second constant-temperature water bath box are arranged on one side of the feeding mechanism, a culture tank is arranged inside the aseptic operation room, a control console is arranged outside the main body, and a display screen is arranged on the control console.
Further preferably, the water temperature of the first constant temperature water bath tank is constant at 40-45 ℃.
Further preferably, the water temperature of the second constant temperature water bath tank is constant at 0-5 ℃.
Preferably, the feeding mechanism comprises a feeding box, the feeding box is communicated with the sterile operation room, a sliding rail is arranged in the feeding box, a moving platform is arranged on the sliding rail, and a sealing door is arranged between the sterile operation room and the feeding box. The feeding mechanism is used for placing the test tube containing the probiotic raw material and the temperature control test tube on the moving platform and entering the sterile operation room.
Preferably, the mobile platform is provided with a plurality of fixing holes, and test tubes are inserted into the fixing holes.
The mobile platform is used for fixing and conveying test tubes
Preferably, the pipetting mechanism comprises a first triaxial movement mechanism, and the tail end of the first triaxial movement mechanism is connected with a manipulator. The pipetting mechanism is used for moving the test tube or moving the liquid in the test tube.
Preferably, one side of the manipulator is provided with a plurality of cap screwing mechanisms, and the number and the positions of the cap screwing mechanisms are the same as the number and the positions of the test tubes on the moving platform. The cap screwing mechanism is used to rotate the cap onto the test tube and facilitate lifting of the test tube.
Preferably, a temperature sensor is arranged at the bottom of one of the plurality of cover screwing mechanisms. The temperature sensor is used to measure the temperature in the test tube.
Preferably, a liquid-transferring gun head is arranged on the other side of the manipulator, and an extrusion mechanism is arranged above the liquid-transferring gun head. The pipette tip is used to drop the liquid in the test tube onto the culture medium.
Preferably, a plurality of culture mediums are arranged on the culture tank.
Preferably, a second triaxial movement mechanism is arranged above the culture tank, and a microscopic camera is arranged at the tail end of the second triaxial movement mechanism. The micro camera is used for detecting the flora image on the culture medium, and is electrically connected with the control console and the display screen to display the flora image in the display screen.
Preferably, the top of the aseptic operation room is provided with an ultraviolet lamp, one side of the aseptic operation room is provided with an ozone conveying channel, the other side of the aseptic operation room is provided with an exhaust pipeline, and the exhaust pipeline is provided with an exhaust fan. After detection, the ultraviolet lamp is started, the ozone conveying channel is started, ozone is conveyed, sterilization is carried out on the inside of the sterile operating room, and the sterile environment in the sterile operating room is ensured.
The beneficial effects are that:
1. the utility model provides a device for detecting the heat resistance of a probiotic colony, which can automatically detect the heat resistance of the probiotic colony, is always in a sterile environment and has more accurate detection; and the sterile operation room can be automatically sterilized after operation, so that the next detection is facilitated.
2. In the prior art, the detection of the heat resistance of probiotics is lacking, the detection of the heat resistance of the probiotics can be automatically completed, the test tube is covered by the cover-screwing mechanism and lifted up to be placed into the first water bath box for heating, and the cover-screwing mechanism lifts up the test tube to be placed into the second water bath box for cooling when the temperature sensor detects the specified temperature. And then the liquid in the test tube is discharged onto the culture medium through the liquid-transferring gun head, and the number of the bacterial colony on the culture medium is detected through the microscopic camera. The whole detection process is automatically carried out in a sterile operation room, the operation speed is high, and the detection result is accurate.
3. A sealing door is arranged between the aseptic operation chamber and the feeding box, so that the aseptic operation chamber can be sealed, and external bacteria are prevented from entering the aseptic operation chamber to influence the detection result.
4. At least two test tubes are placed on the moving platform, one test tube is used for being matched with a temperature sensor to serve as a temperature control sample, the other test tubes are detection samples, the test tubes are placed into the first water bath box at the same time to be heated, the temperature control sample is the same as the temperature of the detection samples, and the temperature of the temperature control samples detected by the temperature sensor is the temperature of the detection samples, so that the temperature of the detection samples can be controlled and detected conveniently.
5. After detection, the ultraviolet lamp is started, the ozone conveying channel is started, ozone is conveyed, the whole inorganic operation room is filled with ozone, the inside of the aseptic operation room is sterilized, the aseptic environment in the aseptic operation room is ensured, and the ozone is discharged from the exhaust pipeline.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of an embodiment of a device for detecting heat resistance of a probiotic bacteria population according to the present utility model.
Fig. 2 is a schematic diagram illustrating an internal structure of an apparatus for detecting heat resistance of a probiotic bacteria according to an embodiment of the present utility model.
Fig. 3 is a schematic structural diagram of a pipetting mechanism according to an embodiment of the device for detecting heat resistance of probiotic bacteria.
Fig. 4 is a cross-sectional view of an embodiment of a device for detecting heat resistance of a probiotic bacteria population according to the present utility model.
In the figure: 1-main body, 2-sterile operating room, 21-ultraviolet lamp, 22-ozone conveying channel, 23-exhaust pipeline, 24-exhaust fan, 3-pipetting mechanism, 31-first triaxial motion mechanism, 32-manipulator, 33-cap screwing mechanism, 34-temperature sensor, 35-pipetting gun head, 36-extrusion mechanism, 4-feeding mechanism, 41-feeding box, 42-slide rail, 43-moving platform, 44-sealing door, 45-test tube, 5-first constant temperature water bath, 6-second constant temperature water bath, 7-culture tank, 71-culture medium, 72-second triaxial motion machine, 73-microscopic camera, 8-control desk, 9-display screen and 10-controller.
Detailed Description
The contents of the present utility model can be more easily understood by referring to the following detailed description of preferred embodiments of the present utility model and examples included. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. In case of conflict, the present specification, definitions, will control.
Example 1:
the device for detecting the heat resistance of the probiotics group is shown in the figures 1-4, the raw materials and the enzymolysis liquid are poured into the stirring tank, so that the liquid can be fully stirred and mixed, a good stirring effect is achieved, the PH value during enzymolysis can be automatically adjusted by adding alkaline water, and meanwhile, floating foam generated during stirring can be removed, so that the device has a good enzymolysis effect.
An apparatus for detection of heat resistance of a probiotic population, comprising: the aseptic technique comprises a main body 1, wherein an aseptic technique chamber 2 is arranged in the main body 1, a pipetting mechanism 3 is arranged above the inside of the aseptic technique chamber 2, a feeding mechanism 4 is arranged on one side of the main body 1, a first constant-temperature water bath 5 and a second constant-temperature water bath 6 are arranged on one side of the feeding mechanism 4, a culture tank 7 is arranged in the aseptic technique chamber 2, a control console 8 is arranged on the outer side of the main body 1, and a display screen 9 is arranged on the control console 8. The water temperature of the first thermostatic waterbath 5 is constant at 45 ℃, and the water temperature of the second thermostatic waterbath 6 is constant at 4 ℃.
The feeding mechanism 4 comprises a feeding box 41, the feeding box 41 is communicated with the sterile operation room 2, a sliding rail 42 is arranged in the feeding box 41, a moving platform 43 is arranged on the sliding rail 42, and a sealing door 44 is arranged between the sterile operation room 2 and the feeding box 41. The movable platform 43 is provided with two fixing holes, and test tubes 45 are inserted into the fixing holes. The loading mechanism 4 is used to place the test tube 45 on the moving platform 43 and into the aseptic processing chamber 2. The movable platform 43 is used for fixing and conveying the test tube 45, the sealing door 44 is opened when the movable platform 43 is conveyed out or conveyed in, and the sealing door 44 is closed for the rest of time, so that the sterile environment in the sterile operation room 2 is ensured.
The pipetting mechanism 3 comprises a first triaxial movement mechanism 31, and a manipulator 32 is connected to the end of the first triaxial movement mechanism 31. Two cap screwing mechanisms 33 are arranged on one side of the manipulator 32, and the number and positions of the cap screwing mechanisms 33 are the same as the number and positions of test tubes 45 on the moving platform 43. The pipetting mechanism 3 is used for moving the cuvette 45 or for moving the liquid in the cuvette 45. One bottom of the plurality of cap screwing mechanisms 33 is provided with a temperature sensor 34. The other side of the manipulator 32 is provided with a liquid-transferring gun head 35, and an extrusion mechanism 36 is arranged above the liquid-transferring gun head 35. The cap screwing mechanism 33 is used to rotate the cap onto the test tube 45 and to facilitate lifting of the test tube 45. The temperature sensor 34 is used to measure the temperature in the test tube 45. The pipette tip 35 is used to drop the liquid in the test tube 45 onto the culture medium.
The culture tank 7 is provided with a plurality of culture media 71. A second triaxial moving mechanism 72 is arranged above the culture tank 7, and a microscopic camera 73 is arranged at the tail end of the second triaxial moving mechanism 72. The micro camera 73 is used for detecting a flora image on the culture medium 71, and the micro camera 73 is electrically connected with the console 8 and the display screen 9 to display the flora image in the display screen 9.
An ultraviolet lamp 21 is arranged at the top of the aseptic operation room 2, an ozone conveying channel 22 is arranged on one side of the aseptic operation room 2, an exhaust pipeline 23 is arranged on the other side of the aseptic operation room 2, and an exhaust fan 24 is arranged on the exhaust pipeline 23. After detection, the ultraviolet lamp 21 is started, the ozone conveying channel 22 is started to convey ozone, the interior of the aseptic operation room 2 is sterilized, and the aseptic environment in the aseptic operation room 2 is ensured.
Working principle: two test tubes 45 are placed on the moving platform 43 in the loading box 41, the sealing door 44 is opened, the moving platform 43 slides on the sliding rail 42, and enters the aseptic processing chamber 2, and the sealing door 44 is closed. The first triaxial movement mechanism 31 drives the manipulator 32 to rotate the cap screwing mechanism 33 on the test tube 45, lifts the test tube 45, puts the test tube 45 into the first constant temperature water bath 5 for heating, lifts the test tube 45 into the second constant temperature water bath 6 for cooling after heating for a period of time, and then places the test tube 45 back on the moving platform 43 and unscrews the cap of the test tube 45 after cooling. The pipette tip 35 is placed in the test tube 45, and the pressing mechanism 36 presses the pipette tip 35 to absorb the liquid and moves over the medium 71 to drop the liquid on the medium 71. After a period of incubation, images of the probiotic groups were examined by microscopic camera 73 to detect the number of surviving probiotic groups.
After the detection is finished, the ultraviolet lamp 21 is started, the ozone conveying channel 22 is started to convey ozone, the interior of the aseptic operation room 2 is sterilized, the aseptic environment in the aseptic operation room 2 is ensured, and then the exhaust fan 24 is started to exhaust the ozone from the exhaust pipeline 23.
The foregoing examples are illustrative only and serve to explain some features of the method of the utility model. The appended claims are intended to claim the broadest possible scope and the embodiments presented herein are merely illustrative of selected implementations based on combinations of all possible embodiments. It is, therefore, not the intention of the applicant that the appended claims be limited by the choice of examples illustrating the features of the utility model. Some numerical ranges used in the claims also include sub-ranges within which variations in these ranges should also be construed as being covered by the appended claims where possible.
Claims (10)
1. An apparatus for the detection of heat resistance of a probiotic population, comprising: the automatic incubator comprises a main body, wherein an aseptic operation room is arranged inside the main body, a pipetting mechanism is arranged above the aseptic operation room, a feeding mechanism is arranged on one side of the main body, a first constant-temperature water bath box and a second constant-temperature water bath box are arranged on one side of the feeding mechanism, a culture tank is arranged inside the aseptic operation room, a control console is arranged outside the main body, and a display screen is arranged on the control console.
2. The device for detecting the heat resistance of the probiotics group according to claim 1, wherein the feeding mechanism comprises a feeding box, the feeding box is communicated with a sterile operation room, a sliding rail is arranged in the feeding box, a movable platform is arranged on the sliding rail, and a sealing door is arranged between the sterile operation room and the feeding box.
3. The device for detecting the heat resistance of the probiotics group according to claim 2, wherein a plurality of fixing holes are formed in the moving platform, and test tubes are inserted into the fixing holes.
4. A device for heat tolerance detection of a probiotic group according to claim 3, wherein the pipetting mechanism comprises a first triaxial movement mechanism, and a manipulator is connected to the end of the first triaxial movement mechanism.
5. The device for detecting the heat resistance of the probiotics group according to claim 4, wherein a plurality of cap screwing mechanisms are arranged on one side of the manipulator, and the number and the positions of the cap screwing mechanisms are the same as those of test tubes on the moving platform.
6. The device for heat tolerance detection of probiotic bacteria according to claim 5, wherein a temperature sensor is provided at the bottom of one of the plurality of cap screwing mechanisms.
7. The device for detecting the heat resistance of the probiotics group according to claim 4, wherein a liquid-transferring gun head is arranged on the other side of the manipulator, and an extrusion mechanism is arranged above the liquid-transferring gun head.
8. The device for detecting the heat resistance of a probiotic group according to claim 1, wherein a plurality of culture mediums are provided on the culture tank.
9. The device for detecting the heat resistance of the probiotics group according to claim 8, wherein a second triaxial movement mechanism is arranged above the culture tank, and a microscopic camera is arranged at the tail end of the second triaxial movement mechanism.
10. The device for detecting the heat resistance of the probiotics group according to claim 1, wherein an ultraviolet lamp is arranged at the top of the aseptic operation room, an ozone conveying channel is arranged on one side of the aseptic operation room, an exhaust pipeline is arranged on the other side of the aseptic operation room, and an exhaust fan is arranged on the exhaust pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321831732.6U CN220376681U (en) | 2023-07-12 | 2023-07-12 | Device for detecting heat resistance of probiotics group |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321831732.6U CN220376681U (en) | 2023-07-12 | 2023-07-12 | Device for detecting heat resistance of probiotics group |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220376681U true CN220376681U (en) | 2024-01-23 |
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ID=89560564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321831732.6U Active CN220376681U (en) | 2023-07-12 | 2023-07-12 | Device for detecting heat resistance of probiotics group |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220376681U (en) |
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2023
- 2023-07-12 CN CN202321831732.6U patent/CN220376681U/en active Active
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