CN220317836U - Bioreactor for stem cell exosome - Google Patents

Abstract

The utility model discloses a bioreactor for stem cell exosomes, which relates to the technical field of cell treatment and comprises a box body, wherein the front surface of the box body is movably connected with a box door, the center of one side of the inner wall of the box body is fixedly connected with a motor, one side of a rotating disc is close to a driving plate, and the inside of a placing frame is movably connected with a culture tank. According to the utility model, the motor drives the rotating disc to rotate, so that the driving rod slides in the circular arc shape along the inside of the driving groove, the driving plate and the placement frame are driven to shake, the other end of the placement frame is supported by the telescopic spring as a buffer, the shaking effect of the device is improved, the placement frame is supported by the strip-shaped plate, meanwhile, the supporting rods are inserted into the two ends of the culture groove as limiting and supporting, the culture groove can be conveniently placed and taken, the culture dish is positioned in the culture groove, and overflowed culture liquid can flow into the culture groove, so that the device effectively reduces the overflow of the culture liquid and the collection of the overflowed culture liquid, and the waste of the culture liquid is reduced.

Description

Bioreactor for stem cell exosome

Technical Field

The utility model relates to the technical field of cell treatment, in particular to a bioreactor for stem cell exosomes.

Background

Exosomes are microvesicles secreted by stem cells. They resemble intercellular "communication soldiers" that can transfer DNA, RNA or proteins between cells, thereby affecting the function of the recipient cell. In the field of stem cells and regenerative medicine, exosomes are being used for treating diseases ranging from heart diseases to respiratory diseases, and when exosomes are studied, it is sometimes necessary to culture exosomes in a bioreactor first to increase the culture density of cell unit volume, and animal cell culture bioreactors are divided into stirred cell culture bioreactors and non-stirred cell culture bioreactors according to whether stirring paddles are present inside the animal cell culture bioreactors, and the non-stirred cell culture bioreactors mainly adopt modes such as vibration, shaking, rolling or swinging to enable culture liquid to move, so as to keep the suspension state of cells and the gas exchange of the culture liquid.

When the device is reacted, the culture solution is moved in a shaking way, the culture solution in the culture dish possibly overflows to the outside of the culture dish in the shaking process, so that cells in the culture solution overflows to the outside of the culture dish along with the culture solution, the waste of the culture solution and the cells is caused, and the culture dish in the existing device is inconvenient to take after the reaction, and in the light of the fact, the bioreactor for stem cell exosomes is provided.

Disclosure of Invention

The utility model aims to make up the defects of the prior art and provides a bioreactor for stem cell exosomes.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a bioreactor for stem cell exosome, includes the box, box front surface swing joint has the chamber door, and the spout has all been seted up to box inner wall both sides, box inner wall one side center fixedly connected with motor, and motor one end is through rotating end fixedly connected with rolling disc, rolling disc one side is close to the drive plate, and drive plate one side fixedly connected with multiunit rack, the inside swing joint of rack has the culture tank, and the inside top fixedly connected with culture dish of culture tank.

Above-mentioned, the box is whole to be the rectangle, and box front surface one side is connected with the chamber door through the pivot rotation, chamber door one side fixedly connected with handle, inside swing joint of box has drive plate, rack and culture tank, and drive plate one side is close to the box inner wall.

Above-mentioned, both ends all fixedly connected with locating lever about the drive plate, and drive slot has been seted up at drive plate one side center, the drive lever of being close to edge fixedly connected with of rolling disc one side, and drive lever one end activity links up in the drive slot is inside.

The upper end and the lower end of the driving plate movably penetrate through the upper end and the lower end of the box body through the positioning rod, and gaps are formed between the upper end and the lower end of the driving plate and the upper end and the lower end of the placing frame and the box body.

Above-mentioned, rack both sides are all fixedly connected with backup pad, and backup pad one side fixedly connected with supporting shoe, the backup pad passes through the supporting shoe sliding engagement in the spout inside, and supporting shoe bottom fixedly connected with extension spring.

Above-mentioned, the rack comprises a plurality of strip shaped plates, and the inside both sides of rack are all fixedly connected with bracing piece, strip shaped plate top swing joint has the culture tank, and bracing piece one end activity alternates in culture tank both sides center.

Above-mentioned, the arc is domatic has all been seted up to culture tank inner wall both sides, and the equal fixedly connected with dog in culture tank top both sides, the culture dish is whole trapezoidal, and culture dish both ends and culture tank inner wall fixed connection.

Compared with the prior art, the bioreactor for stem cell exosomes has the following beneficial effects:

1. according to the utility model, the refrigerator door is opened to insert the culture tank into the rack, one side of the rack is fixedly connected with the driving plate, the driving groove is formed in the center of the inside of the driving plate, one side of the rotating disc is movably inserted into the driving groove through the driving rod, the rotating disc is driven to rotate through the motor, so that the driving rod slides in an arc shape along the inside of the driving groove, the driving plate and the rack are driven to shake, the positioning rod is used for limiting, the driving plate can shake up and down, meanwhile, the supporting plate and the supporting block are matched, the other end of the rack is supported by the telescopic spring as a buffer, the shaking effect of the device is improved, nutrient solution in the culture tank and the culture dish is enabled to move to react, the rack is used for driving a plurality of culture tanks and the culture dish, and the reaction efficiency of the device is improved.

2. The utility model utilizes the integral structure of the placing frame, the placing frame is supported by the strip-shaped plates, and meanwhile, the supporting rods are inserted into the two ends of the culture tank to be limited and supported, so that the culture tank can be placed and taken conveniently, the device is beneficial to shaking the culture tank, and the nutrient solution in the culture tank is not easy to overflow when the culture tank is in shaking by utilizing the trapezoid structure of the culture tank, and the overflowed culture solution flows into the culture tank, thereby effectively reducing the overflow of the culture solution and the collection of the overflowed culture solution by the device and reducing the waste of the culture solution.

Additional advantages, objects, and features of the utility model will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the utility model.

Drawings

FIG. 1 is a schematic perspective view of the present utility model;

FIG. 2 is a schematic view of a sectional partial structure of the present utility model;

FIG. 3 is a schematic view of a partial structure of a rotating disk and a driving plate according to the present utility model;

FIG. 4 is a schematic side sectional partial structure of the present utility model;

FIG. 5 is a schematic view of the enlarged partial structure of FIG. 4A according to the present utility model;

FIG. 6 is a schematic view of a partial structure of a rack according to the present utility model;

FIG. 7 is a schematic cross-sectional view of a culture tank according to the present utility model.

In the figure: 1. a case; 2. a door; 3. a chute; 4. a motor; 5. a rotating disc; 501. a driving rod; 6. a driving plate; 601. a positioning rod; 602. a driving groove; 7. a placing rack; 701. a support plate; 702. a support block; 703. a telescopic spring; 704. a support rod; 8. a culture tank; 801. an arc slope; 802. a stop block; 9. a culture dish.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1-7, the present utility model provides a technical solution: the utility model provides a bioreactor for stem cell exosome, includes box 1, box 1 front surface swing joint has chamber door 2, and spout 3 has all been seted up to box 1 inner wall both sides, box 1 inner wall one side center fixedly connected with motor 4, and motor 4 one end is through rotating end fixedly connected with rolling disc 5, rolling disc 5 one side is close to drive plate 6, and drive plate 6 one side fixedly connected with multiunit rack 7, the inside swing joint of rack 7 has culture tank 8, and the inside top fixedly connected with culture dish 9 of culture tank 8.

Utilize the overall structure of device, place the culture tank 8 interlude in rack 7 inside through opening chamber door 2, and rack 7 one side fixedly connected with drive plate 6, drive slot 602 has been seted up at the inside center of drive plate 6, drive 5 one side of rolling disc is through actuating lever 501 activity interlude in inside of drive slot 602, it rotates to drive rolling disc 5 through motor 4, make actuating lever 501 carry out convex slip along inside of drive slot 602, thereby drive plate 6 and rack 7 rock, spacing by locating lever 601, drive plate 6 can rock from top to bottom, cooperation backup pad 701 and supporting shoe 702 simultaneously, by extension spring 703 as the buffer support at the rack 7 other end, improve the device and rock the effect, make culture tank 8 and the inside nutrient solution motion of culture dish 9, react, drive a plurality of culture tanks 8 and culture dish 9 through rack 7, improve the device reaction efficiency, utilize the overall structure of rack 7 through the strip as the support, simultaneously with the bracing piece 704 as prescribing a limit and support in culture tank 8 both ends, make things convenient for culture tank 8 to place and take, be favorable to the device to rock culture tank 8, utilize the extension spring 703 to be regarded as buffer support, the inside of culture tank 9 is located in the inside of culture tank 9, and the inside of overflow liquid is not had the overflow liquid to be wasted, and the inside of culture tank 9 is located in the overflow liquid is reduced, and the overflow liquid is wasted in the inside the culture tank is not had, and is not rocked to the inside to the culture tank 9.

As shown in fig. 1-5, the whole box 1 is rectangular, and box 1 front surface one side rotates through pivot and chamber door 2 to be connected, chamber door 2 one side fixedly connected with handle, box 1 inside swing joint has drive plate 6, rack 7 and culture tank 8, and drive plate 6 one side is close to box 1 inner wall, both ends all fixedly connected with locating lever 601 about drive plate 6, and drive slot 602 has been seted up at drive plate 6 one side center, the edge fixedly connected with drive lever 501 is close to rotating disk 5 one side, and drive lever 501 one end activity links up in drive slot 602 inside, both ends all pass through the locating lever 601 activity about drive plate 6 are in the inside upper and lower both ends of box 1, and drive plate 6 and rack 7 both ends all are equipped with the clearance with box 1 inside, rack 7 both sides all fixedly connected with backup pad 701, and backup pad 701 one side fixedly connected with supporting shoe 702, backup pad 701 passes through the supporting shoe 702 sliding engagement in spout 3 inside, and supporting shoe 702 bottom fixedly connected with extension spring 703.

The culture tank 8 is inserted and placed inside the placement frame 7 by opening the box door 2, the driving plate 6 is fixedly connected to one side of the placement frame 7, the driving groove 602 is formed in the center inside the driving plate 6, one side of the rotating disc 5 is movably inserted inside the driving groove 602 through the driving rod 501, the rotating disc 5 is driven to rotate through the motor 4, the driving rod 501 slides in an arc shape along the inside of the driving groove 602, the driving plate 6 and the placement frame 7 are driven to shake, the positioning rod 601 is used for limiting, the driving plate 6 can shake up and down, the supporting plate 701 and the supporting block 702 are matched, the other end of the placement frame 7 is supported by the telescopic spring 703 as a buffer, the shaking effect of the device is improved, the culture tank 8 and the culture dish 9 are enabled to move in nutrient solution, the device is reacted, the placement frame 7 drives the culture tanks 8 and the culture dishes 9, and the reaction efficiency of the device is improved.

As shown in fig. 2, 6 and 7, the rack 7 is composed of a plurality of strip-shaped plates, the two sides of the inside of the rack 7 are fixedly connected with supporting rods 704, the top ends of the strip-shaped plates are movably connected with culture tanks 8, one ends of the supporting rods 704 are movably inserted into the centers of the two sides of the culture tanks 8, arc-shaped slopes 801 are formed on the two sides of the inner wall of the culture tanks 8, the two sides of the top ends of the culture tanks 8 are fixedly connected with stop blocks 802, the whole culture dish 9 is trapezoid, and the two ends of the culture dish 9 are fixedly connected with the inner wall of the culture tanks 8.

Utilize rack 7's overall structure, rack 7 is through the strip shaped plate as supporting, and it is prescribing a limit to and support to alternate bracing piece 704 in culture tank 8 both ends simultaneously, make things convenient for culture tank 8 to place and take, be favorable to the device to rock culture tank 8, utilize the trapezium structure of culture dish 9, inside nutrient solution of culture dish 9 is difficult for taking place to overflow when rocking the messenger, and culture dish 9 is located culture tank 8 inside, the overfall culture solution can flow to culture tank 8 inside, thereby make the device effectively reduce the culture solution excessive and collect the overfall culture solution, it is extravagant to reduce the culture solution.

Working principle: utilize the overall structure of device, place the culture tank 8 interlude in rack 7 inside through opening chamber door 2, and rack 7 one side fixedly connected with drive plate 6, drive slot 602 has been seted up at the inside center of drive plate 6, drive plate 5 one side is passed through the activity of actuating lever 501 and is alternateed in inside drive slot 602, it rotates to drive rotary plate 5 through motor 4, make actuating lever 501 carry out convex slip along the inside of drive slot 602, thereby drive plate 6 and rack 7 rock, spacing by locating lever 601, drive plate 6 can rock from top to bottom, cooperate backup pad 701 and supporting shoe 702 simultaneously, by extension spring 703 as the buffering support at the rack 7 other end, improve the device and rock the effect, utilize the overall structure of rack 7, rack 7 passes through the strip shaped plate as the support, at the same time with bracing piece 704 interlude in the culture tank 8 both ends as prescribing and support, make things convenient for culture tank 8 to place and take, be favorable to the device to rock culture tank 8, utilize the trapezium structure of culture dish 9, inside nutrient solution is difficult to take place the overflow when rocking, and culture dish 9 is located inside culture tank 8, the waste fluid that overflows to the inside culture tank 8.

It should be noted that, in this document, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element to be referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless explicitly specified and limited otherwise, the terms "fixedly attached," "mounted," "connected," and "coupled" are to be construed broadly, e.g., as a fixed connection, as a removable connection, or as an integral connection; "coupled" may be either mechanical or electrical; the "connection" may be direct, indirect via an intermediary, or communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. The bioreactor for stem cell exosomes comprises a box body (1), and is characterized in that: the novel incubator is characterized in that the front surface of the incubator body (1) is movably connected with the incubator door (2), sliding grooves (3) are formed in two sides of the inner wall of the incubator body (1), a motor (4) is fixedly connected to one side center of the inner wall of the incubator body (1), one end of the motor (4) is fixedly connected with a rotating disc (5) through a rotating end, one side of the rotating disc (5) is close to a driving plate (6), a plurality of groups of placing racks (7) are fixedly connected to one side of the driving plate (6), a culture groove (8) is movably connected to the inside of the placing racks (7), and a culture dish (9) is fixedly connected to the top end of the inside of the culture groove (8).

2. A bioreactor for stem cell exosomes as claimed in claim 1, wherein: the whole rectangle that is of box (1), and box (1) front surface one side is connected with chamber door (2) rotation through the pivot, chamber door (2) one side fixedly connected with handle, box (1) inside swing joint has drive plate (6), rack (7) and culture tank (8), and drive plate (6) one side is close to box (1) inner wall.

3. A bioreactor for stem cell exosomes as claimed in claim 1, wherein: the upper end and the lower end of the driving plate (6) are fixedly connected with positioning rods (601), a driving groove (602) is formed in the center of one side of the driving plate (6), a driving rod (501) is fixedly connected to one side of the rotating disc (5) close to the edge, and one end of the driving rod (501) is movably connected inside the driving groove (602).

4. A bioreactor for stem cell exosomes as claimed in claim 3, wherein: the upper end and the lower end of the driving plate (6) movably penetrate through the upper end and the lower end of the inside of the box body (1) through the positioning rod (601), and gaps are formed between the upper end and the lower end of the driving plate (6) and the upper end and the lower end of the placing frame (7) and the inside of the box body (1).

5. A bioreactor for stem cell exosomes as claimed in claim 1, wherein: the support plate (701) is fixedly connected to two sides of the placement frame (7), the support block (702) is fixedly connected to one side of the support plate (701), the support plate (701) is slidably connected to the inside of the sliding groove (3) through the support block (702), and the telescopic spring (703) is fixedly connected to the bottom end of the support block (702).

6. A bioreactor for stem cell exosomes as claimed in claim 1, wherein: the rack (7) is composed of a plurality of strip-shaped plates, the two sides of the interior of the rack (7) are fixedly connected with supporting rods (704), the top ends of the strip-shaped plates are movably connected with culture tanks (8), and one ends of the supporting rods (704) are movably inserted into the centers of the two sides of the culture tanks (8).

7. A bioreactor for stem cell exosomes as claimed in claim 1, wherein: arc slope (801) have all been seted up to culture tank (8) inner wall both sides, and culture tank (8) top both sides all fixedly connected with dog (802), culture dish (9) wholly are trapezoidal, and culture dish (9) both ends and culture tank (8) inner wall fixed connection.