CN220224194U - Natural killer cell culture apparatus - Google Patents
Natural killer cell culture apparatus Download PDFInfo
- Publication number
- CN220224194U CN220224194U CN202321331944.8U CN202321331944U CN220224194U CN 220224194 U CN220224194 U CN 220224194U CN 202321331944 U CN202321331944 U CN 202321331944U CN 220224194 U CN220224194 U CN 220224194U
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- China
- Prior art keywords
- fixed
- constant temperature
- cell culture
- temperature incubator
- killer cell
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- 238000004113 cell culture Methods 0.000 title claims abstract description 25
- 210000000822 natural killer cell Anatomy 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 239000002699 waste material Substances 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 4
- 238000012258 culturing Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000009610 hypersensitivity Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000011277 treatment modality Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell culture and discloses a natural killer cell culture device which comprises a constant temperature incubator, wherein both sides of the front surface of the constant temperature incubator are hinged with a box door, the inner wall of the constant temperature incubator is rotationally connected with a rotating rod, the inner side of the rotating rod is fixedly provided with a placing plate, and the top of the placing plate is fixedly provided with a plurality of fixing structures; according to the utility model, the culture dishes can be clamped by the first springs and the clamping plates, and the killer cell culture dishes can be intensively cleaned by the classification liquid storage tanks and the split cleaning pipes, so that the uniform cleaning effect can be ensured, the cleaning speed of the culture dishes is greatly improved, the waste liquid tank at the bottom and the rotatable placing plates can be used for rapidly dumping and collecting the cleaning liquid, and the problems that the efficiency is low and the cleaning effect is not uniform when a worker cleans a plurality of culture dishes, and the natural killer cell culture work is not facilitated are solved.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a natural killer cell culture device.
Background
Natural killer cells are important immune cells of the organism, are not only related to anti-tumor, antiviral infection and immune regulation, but also participate in the occurrence of hypersensitivity and autoimmune diseases in some cases, can identify target cells and killing media, and the incidence of cancers in recent years has shown a straight line rising trend. With the development of tumor biology, molecular biology and immunology, immunotherapy has become a fourth tumor treatment modality following traditional tumor treatment methods (surgery, chemotherapy, radiotherapy).
At present, a special culture dish is adopted for culturing cells, and natural killer cells are cultured in the culture way, so that the current cell culture device is required to be cleaned again after being used, a plurality of culture dishes are required to be cleaned simultaneously when the batch natural killer cells are cultured, the efficiency of staff is lower and the cleaning effect is not uniform when the plurality of culture dishes are cleaned, and the next cell sheet culture effect can be influenced if suspended cells attached to the inner wall of the culture device cannot be thoroughly cleaned, so that the natural killer cell culture work is not facilitated.
Disclosure of Invention
The utility model aims to provide a natural killer cell culture device which can intensively clean a killer cell culture dish, so that uniform cleaning effect can be ensured, and the cleaning speed of the culture dish is greatly improved.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a natural killer cell culture apparatus, includes the constant temperature incubator, positive both sides of constant temperature incubator all articulate there is the chamber door, the inner wall rotation of constant temperature incubator is connected with the dwang, the inboard of dwang is fixed with places the board, the top of placing the board is fixed with a plurality of fixed knot constructs, one side of dwang runs through the constant temperature incubator and is fixed with the hand wheel, the fixed surface of dwang has the rotary disk, spacing hole has all been seted up to the both sides of rotary disk, be fixed with limit structure between constant temperature incubator and the rotary disk, the top of constant temperature incubator is fixed with categorised stock solution case, the bottom of categorised stock solution case is fixed with components of a whole that can function independently conveyer pipe, the fixed surface of components of a whole that can function independently conveyer pipe has a plurality of control valves, the bottom intercommunication of components of a whole that can function independently conveyer pipe has the connecting pipe, the bottom intercommunication of connecting pipe has components of a whole that can function independently washs the pipe, the inside bottom of constant temperature incubator is fixed with the waste liquid case.
Preferably, the fixed knot constructs including fixed case, fixed case is fixed in the top of placing the board, the inside both sides of fixed case all sliding connection has the slip post, the inboard of slip post is fixed with the clamping plate, the surface cover of slip post is equipped with first spring.
Preferably, a pull ring is fixed on the outer side of the sliding column, and the pull ring is made of plastic.
Preferably, the limit structure comprises a fixed frame, the fixed frame is fixed in one side of constant temperature incubator, the inside sliding connection of fixed frame has the gag lever post, gag lever post and spacing hole swing joint, one side of gag lever post is fixed with the impeller block, the fixed surface of gag lever post has the limiting plate, the surface cover of gag lever post is equipped with the second spring, the second spring is located between limiting plate and the fixed frame.
Preferably, the front surface of the pushing block is fixed with a plurality of anti-slip strips, and the anti-slip strips are made of rubber materials.
Preferably, support rods are fixed on the periphery of the bottom of the classification liquid storage tank, and the bottom of the support rods is fixedly connected with the constant temperature incubator.
Compared with the prior art, the utility model has the following beneficial effects:
according to the utility model, the culture dishes can be clamped by the first springs and the clamping plates, and the killer cell culture dishes can be intensively cleaned by the classification liquid storage tanks and the split cleaning pipes, so that the uniform cleaning effect can be ensured, the cleaning speed of the culture dishes is greatly improved, the waste liquid tank at the bottom and the rotatable placing plates can be used for rapidly dumping and collecting the cleaning liquid, and the problems that the efficiency is low and the cleaning effect is not uniform when a worker cleans a plurality of culture dishes, and the natural killer cell culture work is not facilitated are solved.
Drawings
FIG. 1 is a schematic perspective view of the present utility model;
FIG. 2 is a schematic perspective view of the present utility model;
FIG. 3 is a schematic view of a partial perspective structure of the present utility model;
FIG. 4 is a schematic view of a partial perspective structure of the present utility model;
fig. 5 is an enlarged view of a portion of fig. 1 a of the present utility model.
In the figure: 1. a constant temperature incubator; 2. a door; 3. a rotating lever; 4. placing a plate; 5. a fixed structure; 51. a fixed box; 52. a sliding column; 53. a clamping plate; 54. a first spring; 6. a hand wheel; 7. a rotating disc; 8. a limiting hole; 9. a limit structure; 91. a fixed frame; 92. a limit rod; 93. a pushing block; 94. a limiting plate; 95. a second spring; 10. classifying the liquid storage tank; 11. a split conveying pipe; 12. a control valve; 13. a connecting pipe; 14. a split cleaning tube; 15. a waste liquid tank; 16. a pull ring; 17. an anti-slip strip; 18. and (5) supporting the rod.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-5, a natural killer cell culturing device comprises a constant temperature incubator 1, wherein the constant temperature incubator 1 adopts the working principle and structure of the existing cell incubator, both sides of the front surface of the constant temperature incubator 1 are hinged with a box door 2, the inner wall of the constant temperature incubator 1 is rotatably connected with a rotating rod 3, the inner side of the rotating rod 3 is fixedly provided with a placing plate 4, the top of the placing plate 4 is fixedly provided with a plurality of fixing structures 5, one side of the rotating rod 3 penetrates through the constant temperature incubator 1 and is fixedly provided with a hand wheel 6, the surface of the rotating rod 3 is fixedly provided with a rotating disc 7, both sides of the rotating disc 7 are provided with limiting holes 8, a limiting structure 9 is fixed between the constant temperature incubator 1 and the rotating disc 7, the top of the constant temperature incubator 1 is fixedly provided with a sorting liquid storage tank 10, the bottom of the sorting liquid storage tank 10 is fixedly provided with a split conveying pipe 11, the surface of the split conveying pipe 11 is fixedly provided with a plurality of control valves 12, the bottom of the split conveying pipe 11 is communicated with a connecting pipe 13, the bottom of the connecting pipe 13 is communicated with a split cleaning pipe 14, and the bottom of the inside of the constant temperature incubator 1 is fixedly provided with a waste liquid tank 15; this culture apparatus can cooperate the clamp plate 53 to press from both sides tightly the culture dish through first spring 54, can concentrate to wash the killer cell culture dish through categorised liquid reserve tank 10 cooperation components of a whole that can function independently wash pipe 14, not only can guarantee that the cleaning performance is unified, improved the washing speed of culture dish by a wide margin moreover, waste liquid case 15 of bottom and placing plate 4 that can rotate can empty fast and collect abluent liquid, the staff has been solved and efficiency is lower and cleaning performance is not unified when wasing a plurality of culture dishes, be unfavorable for natural killer cell culture work's problem.
The fixed knot constructs 5 including fixed box 51, fixed box 51 is fixed in the top of placing board 4, the equal sliding connection in both sides of fixed box 51 inside has slip post 52, the inboard of slip post 52 is fixed with clamping plate 53, the surface cover of slip post 52 is equipped with first spring 54, first spring 54 can cooperate clamping plate 53 to press from both sides the culture dish, can conveniently fix the culture dish and cultivate and wash work, the outside of slip post 52 is fixed with pull ring 16, the material of pull ring 16 is plastics, the pull ring 16 can laminate the finger, make things convenient for the cultivateer to stimulate slip post 52, can press from both sides the culture dish fast.
The limit structure 9 comprises a fixed frame 91, the fixed frame 91 is fixed on one side of the constant temperature incubator 1, a limit rod 92 is connected to the inside of the fixed frame 91 in a sliding manner, the limit rod 92 is movably connected with the limit hole 8, a pushing block 93 is fixed on one side of the limit rod 92, a limit plate 94 is fixed on the surface of the limit rod 92, a second spring 95 is sleeved on the surface of the limit rod 92, the second spring 95 is located between the limit plate 94 and the fixed frame 91, the second spring 95 can drive the limit rod 92 to limit and fix the rotating disc 7, the placing plate 4 can be kept at a horizontal position, a plurality of anti-slip strips 17 are fixed on the front face of the pushing block 93, the anti-slip strips 17 are made of rubber materials, friction force of the pushing block 93 can be improved, and slipping during pushing is avoided.
The support rods 18 are fixed on the periphery of the bottom of the classification liquid storage tank 10, the bottom of the support rods 18 is fixedly connected with the constant temperature incubator 1, the support rods 18 can be connected with the classification liquid storage tank 10 and the constant temperature incubator 1, the classification liquid storage tank 10 is fixed, and the stability of the classification liquid storage tank 10 is improved.
Working principle: when natural killer cell culture is carried out, a worker firstly opens the box door 2, then places collected natural killer cells in a culture dish, then pulls the sliding column 52 outwards, the pull ring 16 can be attached to a finger to pull the sliding column 52, the sliding column 52 drives the clamping plates 53 to squeeze the first springs 54, the culture dish is placed between the clamping plates 53 after the clamping plates 53 are pulled open, the sliding column 52 is loosened, the clamping plates 53 clamp the culture dish under the action of the first springs 54, after clamping, the worker closes the box door 2 to carry out cell culture, after the culture is completed, the worker distributes clear water, diluted hydrochloric acid liquid and detergent into the classification liquid storage box 10, when the culture dish is cleaned, the worker opens the corresponding control valve 12, corresponding liquid enters the split cleaning pipe 14 through the split conveying pipe 11 and the connecting pipe 13, finally enters the culture dish to be soaked, refreshed, pickled and washed, the worker pushes the pushing block 93 to squeeze the second springs 95, and then rotates the rotating rod 3 to pour waste liquid.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A natural killer cell culture apparatus, comprising a constant temperature incubator (1), characterized in that: the utility model provides a constant temperature incubator (1) positive both sides all articulate there is chamber door (2), the inner wall rotation of constant temperature incubator (1) is connected with dwang (3), the inboard of dwang (3) is fixed with places board (4), the top of placing board (4) is fixed with a plurality of fixed knot constructs (5), one side of dwang (3) runs through constant temperature incubator (1) and is fixed with hand wheel (6), the fixed surface of dwang (3) has rotary disk (7), spacing hole (8) have all been seted up to the both sides of rotary disk (7), be fixed with limit structure (9) between constant temperature incubator (1) and rotary disk (7), the top of constant temperature incubator (1) is fixed with categorised liquid reserve tank (10), the bottom of categorised liquid reserve tank (10) is fixed with components of a whole that can function independently conveyer pipe (11), the surface mounting of components of a whole that can function independently conveyer pipe (11) has a plurality of control valves (12), the bottom intercommunication of components of a whole that can function independently conveyer pipe (11) has (13), the bottom intercommunication of connecting pipe (13) has components of a whole that can function independently washs (14), the bottom of connecting pipe (1) is fixed with waste liquid (15).
2. The natural killer cell culture apparatus according to claim 1, wherein: the fixing structure (5) comprises a fixing box (51), the fixing box (51) is fixed at the top of the placing plate (4), sliding columns (52) are connected to two sides of the inside of the fixing box (51) in a sliding mode, clamping plates (53) are fixed to the inner sides of the sliding columns (52), and first springs (54) are sleeved on the surfaces of the sliding columns (52).
3. A natural killer cell culture apparatus according to claim 2, wherein: a pull ring (16) is fixed on the outer side of the sliding column (52), and the pull ring (16) is made of plastic.
4. The natural killer cell culture apparatus according to claim 1, wherein: the utility model provides a limit structure (9) is including fixed frame (91), one side that fixed frame (91) was fixed in constant temperature incubator (1), the inside sliding connection of fixed frame (91) has gag lever post (92), gag lever post (92) and spacing hole (8) swing joint, one side of gag lever post (92) is fixed with pushing block (93), the fixed surface of gag lever post (92) has limiting plate (94), the surface cover of gag lever post (92) is equipped with second spring (95), second spring (95) are located between limiting plate (94) and the fixed frame (91).
5. The natural killer cell culture apparatus according to claim 4, wherein: the front of the pushing block (93) is fixedly provided with a plurality of anti-slip strips (17), and the anti-slip strips (17) are made of rubber materials.
6. The natural killer cell culture apparatus according to claim 1, wherein: support rods (18) are fixed on the periphery of the bottom of the classification liquid storage tank (10), and the bottom of each support rod (18) is fixedly connected with the constant temperature incubator (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321331944.8U CN220224194U (en) | 2023-05-29 | 2023-05-29 | Natural killer cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321331944.8U CN220224194U (en) | 2023-05-29 | 2023-05-29 | Natural killer cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220224194U true CN220224194U (en) | 2023-12-22 |
Family
ID=89184804
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321331944.8U Active CN220224194U (en) | 2023-05-29 | 2023-05-29 | Natural killer cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220224194U (en) |
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2023
- 2023-05-29 CN CN202321331944.8U patent/CN220224194U/en active Active
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