CN217757513U - Perfusion culture device of 3D printing hydrogel support - Google Patents
Perfusion culture device of 3D printing hydrogel support Download PDFInfo
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- CN217757513U CN217757513U CN202221117180.8U CN202221117180U CN217757513U CN 217757513 U CN217757513 U CN 217757513U CN 202221117180 U CN202221117180 U CN 202221117180U CN 217757513 U CN217757513 U CN 217757513U
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Abstract
The utility model discloses a 3D prints perfusion culture apparatus of aquogel support, the power distribution box comprises a box body, the observation window has been seted up to the preceding lateral wall of box, the rear side inner wall fixed mounting of box has the cultivation platform, the top of cultivateing the platform is provided with culture apparatus, the inboard bottom of box is provided with the liquid storage pot. Be screw-thread grafting through sealed lid and culture apparatus, make culture apparatus's inside seal, and then prevent to breed the bacterium, set for the heating temperature of hot plate through the controller, nutrient solution heats in the stock solution jar through the hot plate, it rotates to drive the axis of rotation through the motor, it rotates and then realizes carrying out even heating to the nutrient solution in the stock solution jar to drive the paddle through the axis of rotation, through the water pump with the heating back nutrient solution suction of liquid storage pot to the culture apparatus in through the inlet tube, make in culture apparatus circulation back through first waste pipe inflow to the waste water tank, flow in and simulate human environment in culture apparatus through the nutrient solution.
Description
Technical Field
The utility model relates to a technical field is cultivateed in the perfusion, especially relates to a 3D prints perfusion culture apparatus of aquogel support.
Background
The 3Dlife bionic hydrogel system is very similar to an extracellular matrix in a living body, and can be used for basic research, drug screening, regenerative medicine and the like.
The prior perfusion culture device for 3D printing hydrogel stent simulates the environment of human body by adopting nutrient solution flowing, and through patent retrieval, chinese patent CN202022849826.9 also provides a perfusion culture device for 3D printing hydrogel stent containing cells, wherein defects exist, the entering nutrient solution cannot be heated, and thus the environment simulating the temperature cannot be simulated in the environment simulating the human body.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art, the utility model provides a 3D prints perfusion culture apparatus of aquogel support has solved current perfusion culture apparatus and can not heat the nutrient solution that gets into and can not carry out the problem of simulating to temperature environment.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a 3D prints perfusion culture apparatus of aquogel support, the power distribution box comprises a box body, the observation window has been seted up to the preceding lateral wall of box, the rear side inner wall fixed mounting of box has the cultivation platform, the top of cultivating the platform is provided with culture apparatus, the inboard bottom of box is provided with the liquid storage pot, fixed mounting is crossed at the top of liquid storage pot has the motor, the output shaft of motor runs through the top of liquid storage pot and extends to the inside fixed mounting of liquid storage pot and has the axis of rotation, the surface of axis of rotation is provided with the paddle, the inboard bottom of liquid storage pot is provided with the hot plate, culture apparatus's top is provided with sealed lid, the preceding lateral wall of box is provided with the controller.
Preferably, a lateral wall of liquid storage pot is provided with the inlet tube, the free end of inlet tube runs through the bottom of cultivateing the platform and extends to the inside of culture apparatus, the surface mosaic of inlet tube installs the water pump, the top of sealed lid is provided with first waste pipe, the bottom of cultivateing the platform is provided with the second waste pipe, the surface mosaic of second waste pipe installs solenoid electric valve, the inboard bottom of box is provided with the waste water tank, the free end of first waste pipe and second waste pipe is the intercommunication setting with the inside of waste water tank.
Preferably, the quantity of paddle has the multiunit, and the multiunit paddle is annular array and sets up, realizes through the multiunit paddle that the nutrient solution in the liquid storage tank carries out the thermally equivalent.
Preferably, the sealing cover is in a step shape, the surface of the sealing cover is provided with threads, the inner wall of the culture device is provided with internal threads, the sealing cover and the culture device are arranged in a threaded mode, and the sealing cover is fixed with the culture device through the sealing cover so as to prevent bacteria from breeding in the culture device.
Preferably, the inside of controller is provided with temperature control unit and temperature detect unit, and the controller is electric connection with the hot plate and sets up, is convenient for control the temperature of hot plate through the controller.
Preferably, the surfaces of the liquid storage tank and the wastewater tank are respectively provided with a liquid inlet and a liquid outlet, so that the liquid storage tank is convenient to heat, and the wastewater tank is convenient to drain.
Compared with the prior art, the beneficial effects of the utility model are that: this perfusion culture apparatus of 3D printing aquogel support, it pegs graft to be the screw thread with culture apparatus through sealed lid, make culture apparatus's inside seal, and then prevent to breed the bacterium, set for the heating temperature of hot plate through the controller, heat nutrient solution in the liquid storage pot through the hot plate, it rotates to drive the axis of rotation through the motor, it rotates and then realizes carrying out even heating to the nutrient solution in the liquid storage pot to drive the paddle through the axis of rotation, through the water pump with the heating back nutrient solution suction of liquid storage pot to culture apparatus in through the inlet tube, make in culture apparatus circulate back through first waste pipe flow in to the waste water tank, flow in culture apparatus through the nutrient solution and simulate human environment.
Drawings
FIG. 1 is a schematic structural view of an isometric view of the present invention;
fig. 2 is a schematic structural view of the front view of the present invention;
fig. 3 is a schematic structural view of a front sectional view of the present invention;
fig. 4 is a schematic structural view of an enlarged view of a portion a of the present invention.
In the figure: the device comprises a box body 1, an observation window 2, a culture table 3, a culture device 4, a liquid storage tank 5, a motor 6, a rotating shaft 7, blades 8, a heating plate 9, a water inlet pipe 10, a water pump 11, a sealing cover 12, a first waste water pipe 13, a second waste water pipe 14, a waste water tank 15 and a controller 16.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example (b): referring to fig. 1-4, the utility model provides a technical scheme, a perfusion culture device of a 3D printing hydrogel support, which comprises a box body 1, an observation window 2 is arranged on the front side wall of the box body 1, a culture table 3 is fixedly arranged on the inner wall of the rear side of the box body 1, a culture device 4 is arranged on the top of the culture table 3, a liquid storage tank 5 is arranged on the bottom of the inner side of the box body 1, a motor 6 is fixedly arranged on the top of the liquid storage tank 5, an output shaft of the motor 6 penetrates through the top of the liquid storage tank 5 and extends to the inside of the liquid storage tank 5, a rotating shaft 7 is fixedly arranged, blades 8 are arranged on the surface of the rotating shaft 7, a plurality of groups of blades 8 are arranged, the blades 8 are arranged in an annular array, nutrient solution in the liquid storage tank 5 is uniformly heated through the blades 8, a heating plate 9 is arranged on the bottom of the inner side of the liquid storage tank 5, a sealing cover 12 is arranged on the top of the culture device 4, the sealing cover 12 is in a step shape, the surface of the sealing cover 12 is provided with threads, the inner wall of the culture device 4 is provided with internal threads, the sealing cover 12 and the culture device 4 are arranged in a threaded manner, the sealing cover 12 and the culture device 4 are fixed to prevent bacteria from breeding in the culture device 4, the front side wall of the box body 1 is provided with a controller 16, a temperature control unit and a temperature detection unit are arranged in the controller 16, the controller 16 and the heating plate 9 are electrically connected, the temperature of the heating plate 9 is conveniently controlled by the controller 16, one side wall of the liquid storage tank 5 is provided with a water inlet pipe 10, the free end of the water inlet pipe 10 penetrates through the bottom of the culture platform 3 and extends to the interior of the culture device 4, a water pump 11 is embedded in the surface of the water inlet pipe 10, the top of the sealing cover 12 is provided with a first waste water pipe 13, the bottom of the culture platform 3 is provided with a second waste water pipe 14, an electromagnetic control valve is embedded and mounted on the surface of the second waste water pipe 14, a waste water tank 15 is arranged at the bottom of the inner side of the box body 1, the free ends of the first waste water pipe 13 and the second waste water pipe 14 are communicated with the inside of the waste water tank 15, and a liquid inlet and a liquid outlet are respectively formed in the surfaces of the liquid storage tank 5 and the waste water tank 15, so that the liquid storage tank 5 can be heated conveniently, and the waste water tank 15 can be drained conveniently; be screw thread grafting through sealed lid 12 and culture apparatus 4, make culture apparatus 4's inside seal, and then prevent to breed the bacterium, set for the heating temperature of hot plate 9 through controller 16, nutrient solution heats in liquid storage pot 5 through hot plate 9, drive axis of rotation 7 through motor 6 and rotate, drive paddle 8 through axis of rotation 7 and rotate and then realize carrying out even heating to the nutrient solution in liquid storage pot 5, through water pump 11 with the heating back nutrient solution suction of liquid storage pot 5 to culture apparatus 4 in through inlet tube 10, make in culture apparatus 4 circulate in the back through first waste pipe 13 flow in to waste water tank 15, flow in at culture apparatus 4 through the nutrient solution and simulate human environment.
When in use: place aquogel and culture dish in culture apparatus 4, it pegs graft to be the screw thread with sealed lid 12 and culture apparatus 4, make culture apparatus 4's inside seal, and then prevent breeding the bacterium, set for the heating temperature of hot plate 9 through controller 16, heat the interior nutrient solution of liquid storage pot 5 through hot plate 9, it rotates to drive axis of rotation 7 through motor 6, it rotates and then realizes carrying out even heating to the nutrient solution in the liquid storage pot 5 to drive paddle 8 through axis of rotation 7, through water pump 11 with the heating back nutrient solution suction of liquid storage pot 5 to culture apparatus 4 in inlet tube 10, make in culture apparatus 4 circulate back through first waste pipe 13 flow into to waste water tank 15, through nutrient solution circulation in culture apparatus 4 and the environment of simulation human body, through second waste pipe 14 and the electromagnetic control valve of its surface setting and then be convenient for discharging the waste liquid of culture apparatus 4 bottom.
In conclusion, this perfusion culture apparatus of 3D printing aquogel support, it pegs graft to be the screw thread through sealed lid 12 and culture apparatus 4, make culture apparatus 4's inside sealed, and then prevent breeding the bacterium, set for the heating temperature of hot plate 9 through controller 16, heat nutrient solution in the liquid storage pot 5 through hot plate 9, drive axis of rotation 7 through motor 6 and rotate, drive paddle 8 through axis of rotation 7 and rotate and then realize carrying out even heating to the nutrient solution in the liquid storage pot 5, through water pump 11 with the heating back nutrient solution suction of liquid storage pot 5 to culture apparatus 4 in through inlet tube 10, make in culture apparatus 4 circulate back through first waste pipe 13 flow into waste water tank 15, through nutrient solution circulation in culture apparatus 4 and advance and simulate human environment, thereby the problem that current perfusion culture apparatus can not heat the nutrient solution that gets into can not simulate the temperature environment is solved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a perfusion culture apparatus of 3D printing aquogel support, includes box (1), its characterized in that, observation window (2) have been seted up to the preceding lateral wall of box (1), the rear side inner wall fixed mounting of box (1) has culture platform (3), the top of culturing platform (3) is provided with culture apparatus (4), the inboard bottom of box (1) is provided with liquid storage pot (5), fixed mounting is crossed at the top of liquid storage pot (5) has motor (6), the inside fixed mounting that the output shaft of motor (6) runs through the top of liquid storage pot (5) and extends to liquid storage pot (5) has axis of rotation (7), the surface of axis of rotation (7) is provided with paddle (8), the inboard bottom of liquid storage pot (5) is provided with hot plate (9), the top of culture apparatus (4) is provided with sealed lid (12), the preceding lateral wall of box (1) is provided with controller (16).
2. The perfusion culture device of a 3D printing hydrogel support according to claim 1, wherein a water inlet pipe (10) is arranged on one side wall of the liquid storage tank (5), the free end of the water inlet pipe (10) penetrates through the bottom of the culture table (3) and extends to the inside of the culture device (4), a water pump (11) is mounted on the surface of the water inlet pipe (10) in an embedded mode, a first waste water pipe (13) is arranged at the top of the sealing cover (12), a second waste water pipe (14) is arranged at the bottom of the culture table (3), an electromagnetic control valve is mounted on the surface of the second waste water pipe (14) in an embedded mode, a waste water tank (15) is arranged at the bottom of the inner side of the box body (1), and the free ends of the first waste water pipe (13) and the second waste water pipe (14) are communicated with the inside of the waste water tank (15).
3. The perfusion culture device of a 3D printing hydrogel support according to claim 1, wherein there are multiple groups of the paddles (8), and the multiple groups of paddles (8) are arranged in an annular array.
4. The perfusion culture device of the 3D printing hydrogel support according to claim 1, wherein the sealing cover (12) is stepped, the surface of the sealing cover (12) is threaded, the inner wall of the culture device (4) is internally threaded, and the sealing cover (12) and the culture device (4) are installed in a threaded manner.
5. The perfusion culture device of a 3D printing hydrogel support according to claim 1, wherein a temperature control unit and a temperature detection unit are arranged inside the controller (16), and the controller (16) is electrically connected with the heating plate (9).
6. The perfusion culture device for the 3D printing of the hydrogel support according to claim 2, wherein a liquid inlet and a liquid outlet are respectively arranged on the surfaces of the liquid storage tank (5) and the waste water tank (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221117180.8U CN217757513U (en) | 2022-05-10 | 2022-05-10 | Perfusion culture device of 3D printing hydrogel support |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221117180.8U CN217757513U (en) | 2022-05-10 | 2022-05-10 | Perfusion culture device of 3D printing hydrogel support |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217757513U true CN217757513U (en) | 2022-11-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221117180.8U Active CN217757513U (en) | 2022-05-10 | 2022-05-10 | Perfusion culture device of 3D printing hydrogel support |
Country Status (1)
| Country | Link |
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| CN (1) | CN217757513U (en) |
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2022
- 2022-05-10 CN CN202221117180.8U patent/CN217757513U/en active Active
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