CN209778826U - Microorganism constant temperature water bath culture apparatus - Google Patents

Microorganism constant temperature water bath culture apparatus Download PDF

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Publication number
CN209778826U
CN209778826U CN201920153758.7U CN201920153758U CN209778826U CN 209778826 U CN209778826 U CN 209778826U CN 201920153758 U CN201920153758 U CN 201920153758U CN 209778826 U CN209778826 U CN 209778826U
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CN
China
Prior art keywords
liquid separation
nutrient solution
culture
liquid
microorganism
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Expired - Fee Related
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CN201920153758.7U
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Chinese (zh)
Inventor
陈祖翼
黄健
肖代敏
陈秋月
晏亚萍
李琼瑶
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Affiliated Hospital of Zunyi Medical University
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Affiliated Hospital of Zunyi Medical University
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Priority to CN201920153758.7U priority Critical patent/CN209778826U/en
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Publication of CN209778826U publication Critical patent/CN209778826U/en
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Abstract

The utility model discloses a microorganism thermostatic waterbath culture apparatus belongs to biological cultivation technical field. The device mainly comprises a transparent box body, an airflow stirring device, a liquid separation culture device and a power supply device, wherein the airflow stirring device comprises an exhaust fan, a hollow rotating disc and hollow blades; the nutrient solution is stirred by the rotation of the hollow rotating disc, and then air enters the nutrient solution cavity through the round holes on the hollow blades to further stir the nutrient solution, so that the stirring and mixing effects are improved, and the splashing of the nutrient solution is avoided; the culture medium in the transparent box body can be conveniently taken and placed through the mounting plate which is arranged at the rear end of the transparent box body and can slide up and down; the nutrient solution is dispersed through the liquid separation culture device, so that the influence on the culture quality of the microorganisms caused by uneven dispersion of the nutrient solution is avoided; the utility model discloses simple structure, convenient operation, the microbial cultivation is effectual, is fit for a large amount of promotions.

Description

Microorganism constant temperature water bath culture apparatus
Technical Field
The utility model relates to a biological cultivation technical field, concretely relates to microorganism thermostatic waterbath culture apparatus.
background
Microorganisms include bacteria, viruses, fungi, and some small protists, microscopic algae, etc., which are small and closely related to humans. The food can be widely applied to various fields such as food, medicine, industry and agriculture, environmental protection, sports and the like. In the text books in the mainland area of China and Taiwan, microorganisms are classified into the following 8 major groups, bacteria, viruses, fungi, actinomycetes, rickettsia, mycoplasma, chlamydia, and spirochete. Some microorganisms are visible to the naked eye, such as mushrooms, ganoderma lucidum, lentinus edodes, etc., which belong to fungi. Also microorganisms are a class of "non-cellular organisms" consisting of a few components, such as nucleic acids and proteins, but whose survival must be dependent on living cells.
The cultivation of microorganism is common, the cultivation of current microorganism adopts the culture medium to cultivate usually, adopt the cylindricality jar body or flat box body to cultivate promptly, cultivate inefficiency, and the operation is inconvenient, when using the culture medium to cultivate, the temperature is difficult to control, when the required nutrient substance of microorganism existence stirs after placing, the rotation paddle rotates in aqueous, the rotation receives the influence of water resistance, stirring rate is under, make the nutrient solution can not the intensive mixing even, lead to microorganism growth to breed slowly or die easily.
SUMMERY OF THE UTILITY MODEL
To the problem that exists, the utility model provides a microorganism thermostatic waterbath culture apparatus.
The technical scheme of the utility model is that: the utility model provides a microorganism constant temperature water bath culture device, which mainly comprises a transparent box body, an air flow stirring device, a liquid separation culture device and a power supply device, wherein the upper end of the transparent box body is provided with a liquid inlet, a nutrient solution cavity, a liquid separation culture cavity and a constant temperature water bath cavity are sequentially arranged in the transparent box body from top to bottom, the bottom end of the nutrient solution cavity is provided with a liquid outlet funnel, the upper end of the liquid outlet funnel is provided with a buffer grid which is used for buffering the passing nutrient solution, the stirring time of the air flow stirring device for the nutrient solution is increased, the liquid separation culture cavity is communicated and connected with the nutrient solution cavity through the liquid outlet funnel, the bottom end in the liquid separation culture cavity is provided with a receiving plate which is positioned under the liquid outlet funnel, a plurality of culture medium receiving plates are arranged on the receiving plate, a constant temperature heating drawer is arranged in the constant temperature water bath, the inner wall of the constant-temperature heating drawer is provided with a constant-temperature heater; the air flow stirring device comprises an exhaust fan, a hollow rotating disk and a plurality of hollow blades, the hollow rotating disk is arranged in the nutrient solution cavity, the plurality of hollow blades are uniformly distributed on the outer side wall of the hollow rotating disk respectively and are in penetrating connection with the hollow rotating disk, a plurality of round holes are uniformly formed in one side surface of each hollow blade, and the exhaust fan is connected with the hollow rotating disk through a vent pipe; the liquid separation culture device comprises a liquid separation disc and a plurality of liquid separation heads, wherein the liquid separation disc is arranged at the bottom end of the liquid outlet funnel, a plurality of liquid separation ports are formed in the liquid separation disc, the plurality of liquid separation heads are connected with the liquid separation ports through connecting pipes, and the liquid separation heads are respectively arranged above the corresponding culture medium carrying discs; and the power supply device is electrically connected with the constant-temperature heater and the exhaust fan.
Further, the rear end of the transparent box body is provided with a mounting plate, the mounting plate can slide up and down at the rear end of the transparent box body, and the culture medium inside the transparent box body can be conveniently taken and placed.
Furthermore, go into liquid mouthful upper end and articulate there is the apron, when avoiding need not to add nutrient solution, outside pollutant gets into the nutrient solution intracavity, causes inside nutrient solution to pollute.
Further, constant temperature heating drawer inner wall is equipped with the heat preservation, reduces constant temperature heating drawer internal heat loss's speed through the heat preservation, and it is long when guaranteeing to heat.
Further, the entrance of air exhauster is equipped with air cleaner, carries out filtration treatment to the outside air through air cleaner, causes the pollution when avoiding the pollutant in the outside air to stir the nutrient solution.
Further, the branch liquid head is for leaking hopper-shaped structure, and divides the liquid head bottom to be equipped with the branch liquid board, be equipped with a plurality of branch liquid holes on the branch liquid board, just every divides liquid hole department all to be equipped with the branch liquid net, will further disperse through the nutrient solution of every branch liquid hole department through dividing the liquid net, avoids the nutrient solution dispersion inequality, influences the microorganism culture quality.
The utility model discloses a theory of operation does: firstly, the mounting panel that transparent box rear end set up is pulled upwards, accept the dish with the culture medium and put to accepting on the board, then promote the mounting panel downwards and close it, add nutrient solution to the nutrient solution intracavity through going into the liquid mouth, start the air exhauster, draw the outside air suction after air cleaner filters in the hollow rotary disk, drive the rotation of hollow rotary disk, stir the nutrient solution, then the air gets into the nutrient solution intracavity through the round hole on the hollow blade and further stirs the nutrient solution, the nutrient solution after the stirring adds to the culture medium and accepts the dish through dividing the liquid disc, branch liquid head, through adding water in the constant temperature heating drawer, then heat water through constant temperature heater to the microorganism on the culture medium accepts the dish carries out the constant temperature heating.
Compared with the prior art, the utility model discloses beneficial effect does: the utility model provides a microorganism constant temperature water bath culture apparatus, which stirs the nutrient solution by the rotation of a hollow rotating disc, then the air enters into a nutrient solution cavity through a round hole on a hollow blade to further stir the nutrient solution, thereby increasing the stirring and mixing effect and avoiding the splashing of the nutrient solution; the culture medium in the transparent box body can be conveniently taken and placed through the mounting plate which is arranged at the rear end of the transparent box body and can slide up and down; the cover plate hinged to the upper end of the liquid inlet prevents external pollutants from entering the nutrient solution cavity to cause internal nutrient solution pollution when nutrient solution does not need to be added; the heat loss speed in the constant-temperature heating drawer is reduced through the heat-insulating layer, and the heating duration is ensured; the outside air is filtered through the air filter, so that pollution caused when the pollutants in the outside air are stirred into the nutrient solution is avoided; the nutrient solution passing through each liquid separating port is further dispersed through the liquid separating net, so that the influence on the culture quality of microorganisms caused by uneven dispersion of the nutrient solution is avoided; the utility model discloses simple structure, convenient operation, the microbial cultivation is effectual, is fit for a large amount of promotions.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic view of the connection structure between the transparent box and the mounting plate of the present invention;
FIG. 3 is a schematic view of the internal structure of the constant temperature heating drawer of the present invention;
FIG. 4 is a schematic view of the connection structure of the hollow rotating disk and the hollow blades of the present invention;
Fig. 5 is a schematic structural view of the liquid separation plate of the present invention.
The device comprises a transparent box body 1, a liquid inlet 10, a cover plate 100, a nutrient solution cavity 11, a liquid outlet funnel 110, a buffer grid 111, a liquid separating culture cavity 12, a receiving plate 120, a nutrient medium receiving disc 121, a constant temperature water bath cavity 13, a constant temperature heating drawer 130, a constant temperature heater 131, a heat insulating layer 132, a mounting plate 14, an airflow stirring device 2, an exhaust fan 20, an air filter 200, a hollow rotating disc 21, a hollow blade 22, a circular hole 220, a liquid separating culture device 3, a liquid separating disc 30, a liquid separating port 300, a liquid separating head 31, a liquid separating plate 310, a liquid separating hole 311 and a liquid separating net 312.
Detailed Description
Example (b): as shown in figure 1, a microorganism constant temperature water bath culture device mainly comprises a transparent box body 1, an air flow stirring device 2, a liquid separation culture device 3 and a power supply device, wherein a liquid inlet 10 is arranged at the upper end of the transparent box body 1, a cover plate 100 is hinged at the upper end of the liquid inlet 10, so that when no nutrient solution needs to be added, external pollutants enter a nutrient solution cavity 11 to cause internal nutrient solution pollution, a nutrient solution cavity 11, a liquid separation culture cavity 12 and a constant temperature water bath cavity 13 are sequentially arranged in the transparent box body 1 from top to bottom, as shown in figure 2, a mounting plate 14 is arranged at the rear end of the transparent box body 1, the mounting plate 14 can slide up and down at the rear end of the transparent box body 1 to facilitate taking and placing of an internal culture medium, a liquid outlet funnel 110 is arranged at the bottom end of the nutrient solution cavity 11, a buffer grid 111 is arranged at the upper end of the liquid outlet funnel 110, the buffer grid 111 is used, the liquid separation culture cavity 12 is communicated with the nutrient solution cavity 11 through the liquid outlet funnel 110, the bottom end inside the liquid separation culture cavity 12 is provided with the bearing plate 120, the bearing plate 120 is positioned under the liquid outlet funnel 110, the bearing plate 120 is provided with a plurality of culture medium bearing discs 121, the constant-temperature water bath cavity 13 is internally provided with the constant-temperature heating drawer 130, as shown in fig. 3, the constant-temperature heating drawer 130 is positioned under the bearing plate 120, the inner wall of the constant-temperature heating drawer 130 is provided with a constant-temperature heater 131, the inner wall of the constant-temperature heating drawer 130 is provided with a heat preservation layer 132, the heat loss speed in the constant-temperature heating drawer 130 is reduced through the heat; the air flow stirring device 2 comprises an exhaust fan 20, a hollow rotating disc 21 and hollow blades 22, an air filter 200 is arranged at an inlet of the exhaust fan 20, external air is filtered through the air filter 200, and pollution caused when pollutants in the external air are stirred into the nutrient solution is avoided, as shown in fig. 4, the hollow rotating disc 21 is arranged in the nutrient solution cavity 11, a plurality of hollow blades 22 are arranged, the hollow blades 22 are respectively and uniformly distributed on the outer side wall of the hollow rotating disc 21 and are in penetrating connection with the hollow rotating disc 21, a plurality of round holes 220 are uniformly arranged on one side surface of each hollow blade 22, and the exhaust fan 20 is connected with the hollow rotating disc 21 through a vent pipe; the liquid separation culture device 3 comprises a liquid separation disc 30 and a plurality of liquid separation heads 31, wherein the liquid separation disc 30 is arranged at the bottom end of the liquid outlet funnel 110, the liquid separation disc 30 is provided with a plurality of liquid separation ports 300, the liquid separation heads 31 are provided with a plurality of liquid separation heads 31, the liquid separation heads 31 are connected with the liquid separation ports 300 through connecting pipes, the liquid separation heads 31 are respectively arranged above the corresponding culture medium receiving discs 121, the liquid separation heads 31 are of funnel-shaped structures, the bottom ends of the liquid separation heads 31 are provided with liquid separation plates 310, as shown in fig. 5, the liquid separation plates 310 are provided with a plurality of liquid separation holes 311, liquid separation nets 312 are arranged at the liquid separation holes 311, and the nutrient solution passing through the liquid separation holes 311 is further dispersed through the liquid separation nets 312, so that the nutrient solution is prevented from being unevenly dispersed, and the microorganism culture quality is prevented from being influenced; the power supply device is electrically connected with the constant temperature heater 131 and the exhaust fan 20.
the utility model discloses a theory of operation: firstly, the mounting plate 14 arranged at the rear end of the transparent box body 1 is pulled upwards, the culture medium receiving disc 121 is placed on the receiving plate 120, then the mounting plate 14 is pushed downwards to be closed, nutrient solution is added into the nutrient solution cavity 11 through the solution inlet 10, the buffer grid 111 is used for starting the exhaust fan 20, air filtered by the air filter 200 from the outside is sucked into the hollow rotating disc 21, the hollow rotating disc 21 is driven to rotate to stir the nutrient solution, then the air enters the nutrient solution cavity 11 through the round holes 220 on the hollow blades 22 to further stir the nutrient solution, the stirred nutrient solution is added into the culture medium receiving disc 121 through the liquid separating disc 30 and the liquid separating head 31, water is added into the constant temperature heating drawer 130, and then the constant temperature heater 131 is used for heating the water, so that microorganisms on the culture medium receiving disc 121 are heated at constant temperature.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will appreciate that; the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the embodiments of the present invention.

Claims (6)

1. The utility model provides a microorganism thermostatic waterbath culture apparatus which characterized in that: mainly comprises a transparent box body (1), an air flow stirring device (2), a liquid separation culture device (3) and a power supply device, wherein a liquid inlet (10) is arranged at the upper end of the transparent box body (1), a nutrient solution cavity (11), a liquid separation culture cavity (12) and a constant temperature water bath cavity (13) are sequentially arranged in the transparent box body (1) from top to bottom, a liquid outlet funnel (110) is arranged at the bottom end of the nutrient solution cavity (11), a buffer grid (111) is arranged at the upper end of the liquid outlet funnel (110), the liquid separation culture cavity (12) is in through connection with the nutrient solution cavity (11) through the liquid outlet funnel (110), a bearing plate (120) is arranged at the bottom end inside the liquid separation culture cavity (12), the bearing plate (120) is positioned under the liquid outlet funnel (110), a plurality of culture media bearing discs (121) are arranged on the bearing plate (120), and a constant temperature heating drawer (, the constant-temperature heating drawer (130) is positioned right below the bearing plate (120), and a constant-temperature heater (131) is arranged on the inner wall of the constant-temperature heating drawer (130); the air flow stirring device (2) comprises an exhaust fan (20), a hollow rotating disc (21) and a plurality of hollow blades (22), the hollow rotating disc (21) is arranged in the nutrient solution cavity (11), the plurality of hollow blades (22) are respectively and uniformly distributed on the outer side wall of the hollow rotating disc (21) and are in through connection with the hollow rotating disc (21), one side surface of each hollow blade (22) is uniformly provided with a plurality of round holes (220), and the exhaust fan (20) is connected with the hollow rotating disc (21) through a vent pipe; the liquid separation culture device (3) comprises a liquid separation disc (30) and liquid separation heads (31), wherein the liquid separation disc (30) is arranged at the bottom end of the liquid outlet funnel (110), a plurality of liquid separation ports (300) are formed in the liquid separation disc (30), the liquid separation heads (31) are multiple, the liquid separation heads (31) are connected with the liquid separation ports (300) through connecting pipes, and the liquid separation heads (31) are respectively arranged above the corresponding culture medium receiving discs (121); the power supply device is electrically connected with the constant temperature heater (131) and the exhaust fan (20).
2. The apparatus for culturing a microorganism in thermostatic waterbath according to claim 1, characterized in that the transparent case (1) is provided with a mounting plate (14) at the rear end thereof, and the mounting plate (14) can slide up and down on the rear end of the transparent case (1).
3. The apparatus for culturing a microorganism in thermostatic waterbath according to claim 1, characterized in that the upper end of the liquid inlet (10) is hinged with a cover plate (100).
4. The apparatus for culturing a microorganism in thermostatic waterbath according to claim 1, characterized in that the inner wall of the thermostatic heating drawer (130) is provided with an insulating layer (132).
5. The apparatus for the culture of microorganisms in thermostatic waterbath according to claim 1, characterized in that an air filter (200) is provided at the inlet of the suction fan (20).
6. The microorganism culture apparatus with thermostatic waterbath according to claim 1, wherein the liquid separation head (31) is funnel-shaped, a liquid separation plate (310) is arranged at the bottom end of the liquid separation head (31), a plurality of liquid separation holes (311) are arranged on the liquid separation plate (310), and a liquid separation net (312) is arranged at each liquid separation hole (311).
CN201920153758.7U 2019-01-29 2019-01-29 Microorganism constant temperature water bath culture apparatus Expired - Fee Related CN209778826U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201920153758.7U CN209778826U (en) 2019-01-29 2019-01-29 Microorganism constant temperature water bath culture apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201920153758.7U CN209778826U (en) 2019-01-29 2019-01-29 Microorganism constant temperature water bath culture apparatus

Publications (1)

Publication Number Publication Date
CN209778826U true CN209778826U (en) 2019-12-13

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CN201920153758.7U Expired - Fee Related CN209778826U (en) 2019-01-29 2019-01-29 Microorganism constant temperature water bath culture apparatus

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112499811A (en) * 2020-11-12 2021-03-16 江西嘉陶无机材料有限公司 Sewage treatment equipment
CN114480202A (en) * 2022-02-16 2022-05-13 湖北茂盛生物有限公司 Bioremediation microbial inoculum for petroleum-polluted soil, preparation device and method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112499811A (en) * 2020-11-12 2021-03-16 江西嘉陶无机材料有限公司 Sewage treatment equipment
CN112499811B (en) * 2020-11-12 2023-01-06 江西嘉陶无机材料有限公司 Sewage treatment equipment
CN114480202A (en) * 2022-02-16 2022-05-13 湖北茂盛生物有限公司 Bioremediation microbial inoculum for petroleum-polluted soil, preparation device and method

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20191213

Termination date: 20220129

CF01 Termination of patent right due to non-payment of annual fee