CN220951829U - Cell culture temperature control assembly - Google Patents

Abstract

The utility model discloses a cell culture temperature control assembly, and particularly relates to the technical field of cell culture, comprising a culture outer frame, wherein a door frame is hinged on one side of the culture outer frame, a temperature control mechanism is fixedly arranged in the middle of the inner side of the culture outer frame, a placing table is fixedly arranged on one side, close to the door frame, of the culture outer frame, a culture dish is arranged at the top end of the placing table, and a cleaning mechanism is fixedly arranged on one side, far away from the placing table, of the culture outer frame; according to the utility model, by arranging the temperature control mechanism, the liquid guide tube with a planar spiral structure is arranged in the culture dish during cell culture, and the constant-temperature liquid in the liquid guide tube uniformly conducts heat to each part in the culture dish, so that the temperature of each part in the culture dish is controlled, and the temperature difference between the edge position and the middle position of the culture dish is prevented, thereby affecting the overall effect of cell culture.

Description

Cell culture temperature control assembly

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture temperature control assembly.

Background

Cell culture is a manual method of growing and propagating cells in a suitable environment, and common cell culture methods include: culturing in a small amount of suspension, adhesion, debonding, homogenizing, three-dimensional and in vitro; in the cell culture process, the culture effect of cells can be influenced by a plurality of factors, such as temperature and the like, and the temperature control is needed during the cell culture;

The traditional cell culture temperature control assembly is relatively simple in structure and is used for controlling the temperature in a water bath mode, but when the cell culture is carried out, the temperature is controlled in the water bath when the capacity of the culture dish is large because the culture dish is cultivated in a plurality of ways, and the temperature at the edge position and the middle position of the culture dish can have a certain temperature difference, so that the whole effect of the cell culture can be influenced.

Disclosure of utility model

The present utility model is directed to a cell culture temperature control assembly, which solves the above-mentioned problems of the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the cell culture temperature control assembly comprises a culture outer frame, wherein a door frame is hinged to one side of the culture outer frame, a temperature control mechanism is fixedly arranged in the middle of the inner side of the culture outer frame, a placing table is fixedly arranged on one side, close to the door frame, of the culture outer frame, a culture dish is arranged at the top end of the placing table, and a cleaning mechanism is fixedly arranged on one side, far away from the placing table, of the culture outer frame;

The temperature control mechanism comprises a supporting frame, support frame fixed mounting is at the interior lower wall middle part of cultivateing the frame, the middle part rotation of support frame is installed rotatory sleeve, rotatory telescopic middle part slip card is equipped with the lift axle, the top fixed mounting of lift axle has the swivel mount, the equal fixed mounting in both ends of swivel mount has the connecting rod, the equal fixed mounting in bottom of connecting rod has the elevating platform, the bottom of elevating platform all is equipped with the catheter, the catheter is established to planar spiral structure, one end fixed mounting of catheter has first pipe, the other end fixed mounting of catheter has the second pipe, the top fixed joint of first pipe, second pipe is on the elevating platform, the top fixed mounting of first pipe has first valve, the top fixed mounting of second pipe has the second valve, the equal fixed mounting in top of elevating platform has the frame, be equipped with electric heating module on the liquid storage frame, fixed mounting has the third pipe between liquid storage frame's liquid outlet and the first valve, liquid storage frame's income liquid inlet and second valve fixed mounting have the second pipe between the fourth valve.

Preferably, the crown gear is fixedly sleeved on the top of the outer side of the rotary sleeve, a first motor is fixedly installed on one side, close to the crown gear, of the top end of the support frame, a first gear is fixedly installed at the driving end of the first motor, and the first gear is in meshed connection with the crown gear.

Preferably, a first lifting rod is fixedly arranged in the middle of the supporting frame, a bearing is fixedly clamped in the middle of the bottom end of the lifting shaft, and the driving end of the first lifting rod and the middle of the bearing are fixedly arranged.

Preferably, the cleaning mechanism comprises two second lifting rods which are symmetrically distributed, the second lifting rods are fixedly arranged on one side, far away from the placing table, of the inner lower wall of the culture outer frame, an L-shaped frame is fixedly arranged at the driving end of each second lifting rod, a spiral frame is fixedly arranged between the L-shaped frames, a spiral cleaning frame is fixedly arranged at the bottom end of each spiral frame, a plurality of uniformly distributed cleaning perforations are formed in the inner side of each spiral cleaning frame, a spiral drying frame is fixedly arranged at the top end of each spiral frame, a plurality of uniformly distributed drying perforations are formed in the inner side of each spiral drying frame, a first connector is fixedly arranged at one end of each spiral cleaning frame, and a second connector is fixedly arranged at one end of each spiral drying frame.

Preferably, the catheter is matched with the spiral frame, the spiral cleaning frame and the spiral drying frame.

Preferably, the inner lower wall of the culture outer frame is fixedly provided with a cleaning liquid output system matched with the spiral cleaning frame, and a transfusion tube is fixedly arranged between the output end of the cleaning liquid output system and the first connector.

Preferably, a drying air output system matched with the spiral drying frame for use is fixedly arranged on the inner lower wall of the culture outer frame, and an air conveying pipe is fixedly arranged between the output end of the drying air output system and the second connector.

Preferably, a collecting frame is fixedly arranged on one side, close to the spiral cleaning frame, of the inner lower wall of the culture outer frame, the collecting frame is located below the spiral cleaning frame, and a liquid drain pipe is fixedly arranged at the bottom of the collecting frame.

Preferably, the drain pipe fixed joint is in the bottom of cultivateing the frame, the one end that the drain pipe kept away from the collection frame extends the outside of cultivateing the frame, fixedly mounted has the accuse liquid valve on the drain pipe.

Preferably, an observation window is fixedly arranged on the door frame.

Compared with the prior art, the utility model has the beneficial effects that:

1. Through setting up temperature control mechanism, when cell culture, place the catheter of plane helicitic texture in the culture dish, carry out even heat conduction to each position in the culture dish through homothermal liquid in the catheter, the temperature at each position in the control culture dish prevents that culture dish border position and intermediate position temperature from having certain difference in temperature, influences cell culture's overall effect.

2. Through setting up temperature control mechanism, the cooperation uses clean mechanism, adjusts the position of two catheters, does not influence and continues to cell culture temperature control in the next culture dish to carry out self-cleaning, automatic drying to the catheter, be convenient for follow-up catheter place in another culture dish not cause the pollution, promote cell culture's quality.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the structure of the present utility model.

Fig. 2 is a schematic structural view of a temperature control mechanism and a cleaning mechanism in the present utility model.

Fig. 3 is a schematic structural diagram of a temperature control mechanism in the present utility model.

Fig. 4 is an enlarged view of the utility model at a in fig. 3.

Fig. 5 is a schematic diagram illustrating connection of a part of the structure of the temperature control mechanism according to the present utility model.

Fig. 6 is an enlarged view of the present utility model at B in fig. 5.

FIG. 7 is a schematic view showing the structural connection of the cleaning mechanism in the present utility model.

In the figure: 1. culturing the outer frame; 11. a door frame; 12. an observation window; 2. a temperature control mechanism; 3. a placement table; 4. a culture dish; 5. a cleaning mechanism; 21. a support frame; 22. rotating the sleeve; 23. a lifting shaft; 24. a rotating frame; 241. a connecting rod; 25. a lifting table; 26. a catheter; 261. a first tube; 262. a second tube; 27. a first valve; 271. a second valve; 272. a third tube; 273. a fourth pipe; 28. a liquid storage frame; 281. a heating module; 29. a crown gear; 291. a first motor; 292. a first gear; 210. a first lifting rod; 211. a bearing; 51. a second lifting rod; 52. an L-shaped frame; 53. a spiral frame; 501. cleaning the perforation; 502. drying and perforating; 54. a spiral cleaning frame; 541. a first connector; 542. a cleaning liquid output system; 543. an infusion tube; 55. a spiral drying frame; 551. a second connector; 552. a dry air output system; 553. an air delivery pipe; 56. a collection frame; 561. a liquid discharge pipe; 562. and a liquid control valve.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Examples: as shown in fig. 1-7, the utility model provides a cell culture temperature control assembly, which comprises a culture outer frame 1, wherein a door frame 11 is hinged on one side of the culture outer frame 1, and an observation window 12 is fixedly arranged on the door frame 11; the middle part of the inner side of the culture outer frame 1 is fixedly provided with a temperature control mechanism 2, one side of the culture outer frame 1 close to the door frame 11 is fixedly provided with a placing table 3, the top end of the placing table 3 is provided with a culture dish 4, and one side of the culture outer frame 1 far away from the placing table 3 is fixedly provided with a cleaning mechanism 5;

The temperature control mechanism 2 comprises a support frame 21, wherein the support frame 21 is fixedly arranged in the middle of the inner lower wall of the culture outer frame 1, a rotary sleeve 22 is rotatably arranged in the middle of the support frame 21, a lifting shaft 23 is clamped in the middle of the rotary sleeve 22 in a sliding manner, a rotary frame 24 is fixedly arranged at the top end of the lifting shaft 23, connecting rods 241 are fixedly arranged at two end parts of the rotary frame 24, and lifting platforms 25 are fixedly arranged at the bottom ends of the connecting rods 241;

The crown gear 29 is fixedly sleeved on the top of the outer side of the rotary sleeve 22, a first motor 291 is fixedly mounted on one side, close to the crown gear 29, of the top end of the support frame 21, a first gear 292 is fixedly mounted at the driving end of the first motor 291, the first gear 292 is in meshed connection with the crown gear 29, and the first motor 291 is controlled to be started to drive the first gear 292 to drive the crown gear 29 to rotate, so that the rotary sleeve 22, the lifting shaft 23, the rotary frame 24 and the lifting tables 25 on the two sides are driven to rotate;

The middle part of the supporting frame 21 is fixedly provided with a first lifting rod 210, a bearing 211 is fixedly clamped at the middle part of the bottom end of the lifting shaft 23, the driving end of the first lifting rod 210 is fixedly arranged at the middle part of the bearing 211, and the first lifting rod 210 is controlled to be started to drive the lifting shaft 23, the rotating frame 24 and the lifting tables 25 at the two sides to lift;

the bottom end of the lifting table 25 is provided with a liquid guide pipe 26, the liquid guide pipe 26 is of a planar spiral structure, when cells are cultured, the liquid guide pipe 26 is arranged in the culture dish 4, constant-temperature liquid in the liquid guide pipe 26 is used for conducting heat uniformly to all parts in the culture dish 4, the temperature of all the parts in the culture dish 4 is controlled, certain temperature difference between the edge position and the middle position of the culture dish 4 is prevented, the integral effect of cell culture is influenced, one end part of the liquid guide pipe 26 is fixedly provided with a first pipe 261, the other end part of the liquid guide pipe 26 is fixedly provided with a second pipe 262, and the tops of the first pipe 261 and the second pipe 262 are fixedly clamped on the lifting table 25;

The top of the first pipe 261 is fixedly provided with a first valve 27, the top of the second pipe 262 is fixedly provided with a second valve 271, the top of the lifting platform 25 is fixedly provided with a liquid storage frame 28, the liquid storage frame 28 is provided with an electric heating module 281, a third pipe 272 is fixedly arranged between a liquid outlet of the liquid storage frame 28 and the first valve 27, a fourth pipe 273 is fixedly arranged between a liquid inlet of the liquid storage frame 28 and the second valve 271, when the liquid storage frame is used, according to the requirement of the cell culture temperature of the culture dish 4, liquid in the liquid storage frame 28 is heated by the electric heating module 281 to a proper temperature, the first valve 27 and the second valve 271 are opened, the liquid in the liquid storage frame 28 is guided into the liquid guide pipe 26 through the third pipe 272, the first valve 27 and the first pipe 261, and is guided into the liquid storage frame 28 through the second pipe 262, the second valve 271 and the fourth pipe 273, and the constant-temperature liquid in the liquid guide pipe 26 is circulated.

The cleaning mechanism 5 comprises two second lifting rods 51 which are symmetrically distributed, the second lifting rods 51 are fixedly arranged on one side, far away from the placing table 3, of the inner lower wall of the culture outer frame 1, an L-shaped frame 52 is fixedly arranged at the driving end of each second lifting rod 51, a spiral frame 53 is fixedly arranged between the two L-shaped frames 52, a spiral cleaning frame 54 is fixedly arranged at the bottom end of each spiral frame 53, a plurality of uniformly distributed cleaning perforations 501 are formed in the inner side of each spiral cleaning frame 54, a spiral drying frame 55 is fixedly arranged at the top end of each spiral frame 53, a plurality of uniformly distributed drying perforations 502 are formed in the inner side of each spiral drying frame 55, a first connector 541 is fixedly arranged at one end of each spiral cleaning frame 54, a second connector 551 is fixedly arranged at one end of each spiral drying frame 55, and the second lifting rods 51 are controlled to be started to drive the spiral frames 53, the spiral cleaning frames 54 and the spiral drying frames 55 to lift.

The catheter 26 is matched with the spiral frame 53, the spiral cleaning frame 54 and the spiral drying frame 55, and when the spiral frame 53, the spiral cleaning frame 54 and the spiral drying frame 55 are lifted, the catheter 26 is arranged in the spiral frame 53, the spiral cleaning frame 54 and the spiral drying frame 55.

The inner lower wall of the culture outer frame 1 is fixedly provided with a cleaning liquid output system 542 matched with the spiral cleaning frame 54, a transfusion tube 543 is fixedly arranged between the output end of the cleaning liquid output system 542 and the first connector 541, when the culture outer frame is used, the cleaning liquid is controlled to start the cleaning liquid output system 542, the cleaning liquid is guided into the spiral cleaning frame 54 through the transfusion tube 543 and the first connector 541 and is evenly sprayed out through a plurality of cleaning perforations 501, and the surface of the liquid guide tube 26 is automatically cleaned by being matched with the lifting of the spiral cleaning frame 54.

The inner lower wall of the culture outer frame 1 is fixedly provided with a drying air output system 552 matched with the spiral drying frame 55, an air conveying pipe 553 is fixedly arranged between the output end of the drying air output system 552 and the second connector 551, the drying air output system 552 is controlled to be started, the drying air is guided into the spiral drying frame 55 through the air conveying pipe 553 and the second connector 551, and is evenly sprayed out through a plurality of drying perforations 502, the surface of the liquid guide pipe 26 is automatically dried in cooperation with the lifting of the spiral drying frame 55, the subsequent liquid guide pipe 26 is conveniently placed in another culture dish 4 without causing pollution, and the quality of cell culture is improved.

A collecting frame 56 is fixedly arranged on one side, close to the spiral cleaning frame 54, of the inner lower wall of the culture outer frame 1, the collecting frame 56 is positioned below the spiral cleaning frame 54, and a liquid drain pipe 561 is fixedly arranged at the bottom of the collecting frame 56; the drain pipe 561 is fixedly clamped at the bottom of the culture outer frame 1, one end of the drain pipe 561, which is far away from the collecting frame 56, extends out of the culture outer frame 1, a liquid control valve 562 is fixedly arranged on the drain pipe 561, waste liquid generated by cleaning flows into the collecting frame 56 to be collected, and the waste liquid is discharged through the drain pipe 561 by opening the liquid control valve 562.

Working principle: when the liquid guide tube 26 with the planar spiral structure is used, during cell culture, the liquid guide tube 26 is arranged in the culture dish 4, according to the requirement of the cell culture temperature of the culture dish 4, the electric heating module 281 is used for heating the liquid in the liquid storage frame 28 to a proper temperature, the first valve 27 and the second valve 271 are opened, the liquid in the liquid storage frame 28 is led into the liquid guide tube 26 through the third tube 272, the first valve 27 and the first tube 261, and is led into the liquid storage frame 28 through the second tube 262, the second valve 271 and the fourth tube 273, the circulation of constant-temperature liquid in the liquid guide tube 26 is carried out, the constant-temperature liquid in the liquid guide tube 26 is used for uniformly conducting heat to all parts in the culture dish 4, the temperature of all the parts in the culture dish 4 is controlled, and the temperature difference between the edge position and the middle position of the culture dish 4 is prevented, and the whole effect of cell culture is influenced;

after the cell culture in one of the culture dishes 4 is finished, the first lifting rod 210 is controlled to drive the lifting shaft 23, the rotating frame 24 and the lifting tables 25 on the two sides to move upwards to drive the liquid guide tube 26 to be separated from the culture dish 4, and the first motor 291 is controlled to drive the first gear 292 to drive the crown gear 29 to rotate, so that the rotating sleeve 22, the lifting shaft 23, the rotating frame 24 and the lifting tables 25 on the two sides are driven to rotate, and the other liquid guide tube 26 is driven to the position of the placing table 3 to perform subsequent cell culture temperature control;

Simultaneously, the cleaning liquid output system 542 is controlled to be started, the cleaning liquid is led into the spiral cleaning frame 54 through the infusion tube 543 and the first connecting joint 541 and is evenly sprayed out through the plurality of cleaning perforations 501, and the surface of the liquid guide tube 26 is automatically cleaned by matching with the lifting of the spiral cleaning frame 54;

After cleaning, the drying air output system 552 is controlled to be started, the drying air is led into the spiral drying frame 55 through the air conveying pipe 553 and the second connector 551 and is evenly sprayed out through the plurality of drying perforations 502, and the surface of the liquid guide pipe 26 is automatically dried in cooperation with the lifting of the spiral drying frame 55, so that the subsequent liquid guide pipe 26 is conveniently placed in another culture dish 4 without causing pollution, and the quality of cell culture is improved;

The waste liquid generated by the cleaning flows into the collection frame 56 to be collected, and by opening the liquid control valve 562, the waste liquid is discharged through the liquid discharge pipe 561.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. Cell culture temperature control subassembly, including cultivateing frame (1), its characterized in that: a door frame (11) is hinged to one side of the culture outer frame (1), a temperature control mechanism (2) is fixedly arranged in the middle of the inner side of the culture outer frame (1), a placing table (3) is fixedly arranged on one side, close to the door frame (11), of the culture outer frame (1), a culture dish (4) is arranged at the top end of the placing table (3), and a cleaning mechanism (5) is fixedly arranged on one side, far away from the placing table (3), of the culture outer frame (1);

The temperature control mechanism (2) comprises a supporting frame (21), the supporting frame (21) is fixedly arranged in the middle of the inner lower wall of the outer culture frame (1), a rotating sleeve (22) is rotatably arranged in the middle of the supporting frame (21), a lifting shaft (23) is arranged at the middle sliding clamp of the rotating sleeve (22), a rotating frame (24) is fixedly arranged at the top end of the lifting shaft (23), connecting rods (241) are fixedly arranged at the two end parts of the rotating frame (24), lifting platforms (25) are fixedly arranged at the bottom ends of the connecting rods (241), liquid guide pipes (26) are respectively arranged at the bottom ends of the lifting platforms (25), a plane spiral structure is arranged at one end part of each liquid guide pipe (26), a first pipe (261) is fixedly arranged at the other end part of each liquid guide pipe (26), a second pipe (262) is fixedly arranged at the top end part of each first pipe (261) and each second pipe (262) and is fixedly clamped on the lifting platform (25), a first valve (27) is fixedly arranged at the top end part of each first liquid storage pipe (261), a second valve (262) is fixedly arranged at the top end part of each second liquid guide pipe (262), a heating frame (28) is fixedly arranged at the top end part of each heating frame (28), a third pipe (272) is fixedly arranged between the liquid outlet of the liquid storage frame (28) and the first valve (27), and a fourth pipe (273) is fixedly arranged between the liquid inlet of the liquid storage frame (28) and the second valve (271).

2. A cell culture temperature control assembly according to claim 1, wherein: the crown gear (29) is fixedly sleeved on the top of the outer side of the rotary sleeve (22), a first motor (291) is fixedly installed on one side, close to the crown gear (29), of the top end of the support frame (21), a first gear (292) is fixedly installed at the driving end of the first motor (291), and the first gear (292) is meshed with the crown gear (29).

3. A cell culture temperature control assembly according to claim 1, wherein: the lifting device is characterized in that a first lifting rod (210) is fixedly arranged in the middle of the supporting frame (21), a bearing (211) is fixedly clamped in the middle of the bottom end of the lifting shaft (23), and the driving end of the first lifting rod (210) and the middle of the bearing (211) are fixedly arranged.

4. A cell culture temperature control assembly according to claim 1, wherein: the cleaning mechanism (5) comprises two second lifting rods (51) which are symmetrically distributed, the second lifting rods (51) are fixedly installed on one side, far away from the placement table (3), of the inner lower wall of the culture outer frame (1), an L-shaped frame (52) is fixedly installed at the driving end of each second lifting rod (51), a spiral frame (53) is fixedly installed between the L-shaped frames (52), a spiral cleaning frame (54) is fixedly installed at the bottom end of each spiral frame (53), a plurality of uniformly distributed cleaning perforations (501) are formed in the inner side of each spiral cleaning frame (54), a spiral drying frame (55) is fixedly installed at the top end of each spiral frame (53), a plurality of uniformly distributed drying perforations (502) are formed in the inner side of each spiral drying frame (55), a first connector (541) is fixedly installed at one end of each spiral cleaning frame (54), and a second connector (551) is fixedly installed at one end of each spiral drying frame (55).

5. The cell culture temperature control assembly of claim 4, wherein: the inner lower wall of the culture outer frame (1) is fixedly provided with a cleaning liquid output system (542), and a transfusion tube (543) is fixedly arranged between the output end of the cleaning liquid output system (542) and the first connector (541).

6. The cell culture temperature control assembly of claim 4, wherein: the inner lower wall of the culture outer frame (1) is fixedly provided with a drying air output system (552), and an air conveying pipe (553) is fixedly arranged between the output end of the drying air output system (552) and the second connector (551).

7. The cell culture temperature control assembly of claim 4, wherein: the culture outer frame is characterized in that a collection frame (56) is fixedly arranged on one side, close to the spiral cleaning frame (54), of the inner lower wall of the culture outer frame (1), the collection frame (56) is located below the spiral cleaning frame (54), and a liquid drain pipe (561) is fixedly arranged at the bottom of the collection frame (56).

8. The cell culture temperature control assembly of claim 7, wherein: the liquid discharge pipe (561) is fixedly clamped at the bottom of the culture outer frame (1), one end, far away from the collection frame (56), of the liquid discharge pipe (561) extends out of the culture outer frame (1), and a liquid control valve (562) is fixedly installed on the liquid discharge pipe (561).

9. A cell culture temperature control assembly according to claim 1, wherein: an observation window (12) is fixedly arranged on the door frame (11).