CN220183299U - Automatic cell culture system of rotatory rocking formula - Google Patents

Abstract

The utility model relates to a rotary shaking type automatic cell culture system which comprises a shell assembly, a shaking assembly culture assembly and a liquid exchange assembly. Wherein the shell assembly comprises a shell and a sealing cover; the shaking assembly sequentially comprises a motor, a coupler, a rotating shaft and a shaking table from bottom to top, and the upper middle part of the shaking table is connected with the bottom of the inner cavity of the shell through a plurality of springs; the culture assembly comprises a fixed cylinder body, the fixed cylinder body detachably sets the culture dish in the inner cavity of the fixed cylinder body through a plurality of first screw rods, and a filter cartridge is detachably arranged in the culture dish; the liquid exchange component comprises a waste liquid box, a culture liquid box, a liquid taking suction pipe and a liquid exchange suction pipe. According to the utility model, the liquid in the culture dish is changed through the cooperation between the filter cartridge and the liquid changing component, and the cells and the waste liquid are separated through the filter cartridge, so that the liquid changing component is effectively prevented from sucking the cells and directly flushing the cells in the culture dish; the culture dishes with different specifications are matched through the matching between the first screws and the fixed cylinder.

Description

Automatic cell culture system of rotatory rocking formula

Technical Field

The utility model relates to the technical field of cell culture, in particular to a rotary shaking type automatic cell culture system.

Background

Cell culture is also known as cell culture technology. It is an essential process for the whole bioengineering technology. Cell culture is a form of tissue culture in a broad sense, in which organs and tissues are treated with trypsin or the like to isolate cells, which are then transferred to a glass vessel for culture. Cell culture methods can be roughly classified into population culture, cloning culture and drum culture. The population culture is to put a suspension containing a certain number of cells into a culture flask to enable the cells to grow on the wall, and form a uniform single cell layer after confluence; the cloning culture is to add highly diluted free cell suspension into a culture bottle, after each cell is attached to the wall, the distance between each cell is far, and each cell forms a cell colony after growth and proliferation; the drum culture method is continuously rotated during the culture process, so that the cultured cells are always in a suspended state and are not adhered to the wall.

In the process of cell culture, the liquid in the culture dish needs to be shaken, and the liquid in the culture dish is changed periodically. The existing culture device can not shake and change liquid at the same time, and has poor adaptation degree, so that the existing culture device can not adapt to culture dishes with different specifications; the liquid is exchanged in the inner cavity of the culture dish, the straw is easy to suck cells, and the cells and the waste liquid cannot be effectively separated; when new culture solution is discharged, the culture solution is easy to directly wash the cells in the culture dish, which is not beneficial to cell culture; in the process of shaking the culture dish, the shaking amplitude of the culture dish cannot be adjusted.

At present, effective solutions have not been proposed for solving the problems that in the liquid exchange process in the related technology, the suction pipe is easy to suck cells, the culture liquid is easy to directly wash the cells, the shaking amplitude of the culture dish cannot be adjusted, the culture dishes with different specifications cannot be adapted, and the like.

Disclosure of Invention

The utility model aims at overcoming the defects in the prior art, and provides a rotary shaking type automatic cell culture system which solves the problems that cells are easily sucked by a suction pipe, cells are easily washed by culture solution directly, shaking amplitude of a culture dish cannot be adjusted, culture dishes of different specifications cannot be adapted and the like in a liquid exchange process in the related art.

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:

the utility model provides a rotatory automatic culture system that rocks formula cell, includes the casing subassembly and from bottom to top set gradually in the rocking subassembly, the culture assembly and the trading liquid subassembly of casing subassembly inner chamber, wherein:

the shell assembly comprises a shell and a sealing cover detachably arranged at the top end of the shell;

the shaking assembly comprises a motor arranged at the bottom of the inner cavity of the shell, the output end of the motor is connected with a rotating shaft through a coupler, a shaking table is sleeved at the top end of the rotating shaft, and the outer edge of the upper middle part of the shaking table is connected with the bottom of the inner cavity of the shell through a plurality of springs arranged at intervals;

the culture assembly comprises a fixed cylinder body arranged at the center of the top of the shaking table, the fixed cylinder body detachably sets the culture dish in the inner cavity of the fixed cylinder body through a plurality of first screw rods which are assembled with the side wall of the fixed cylinder body in a threaded manner, and a filter cartridge is detachably arranged in the culture dish;

the liquid exchange assembly comprises a liquid taking straw and a liquid exchange straw which are arranged above the filter cylinder, the liquid taking straw is connected with the waste liquid tank through a hose, and the liquid exchange straw is connected with the culture liquid tank through a hose.

Further, a connecting cylinder is arranged at the bottom end of the shaking table, the connecting cylinder is sleeved outside the top end of the rotating shaft, and a plurality of springs are annularly arranged at the middle upper part of the shaking table at equal intervals.

Further, the coupling is detachably connected with the rotating shaft through an adjusting bolt; the adjusting bolt penetrates through the bottom end of the rotating shaft and is in threaded connection with the top end of the coupling.

Further, an adjusting port is formed in the middle lower portion of the shell, a turnover adjusting door is hinged to one side of the adjusting port, and the adjusting port and the adjusting bolt are arranged oppositely.

Further, the fixed cylinder is coaxially arranged with the rotating shaft; the first screws are annularly and equidistantly arranged on the side wall of the fixed cylinder body, and the abutting ends of the first screws are sleeved with rubber sleeves.

Further, a plurality of second screw rods are assembled on the side wall of the middle upper part of the filter cartridge in a threaded manner, and the abutting ends of the second screw rods are positioned outside the filter cartridge and are abutted with the inner wall of the culture dish.

Further, the liquid taking suction pipes and the liquid exchanging suction pipes are symmetrically arranged and are respectively fixed through corresponding supporting frames.

Further, the support frame is 7-shaped, the bottom end of the support frame is fixed on the shell, and the lower end of the support frame is an electric telescopic rod so as to drive the liquid taking suction pipe or the liquid exchanging suction pipe at the top of the support frame to move up and down.

Further, the waste liquid tank and the culture solution tank are respectively arranged at the lower end of the outer wall of the shell.

Further, supporting feet are arranged at four corners of the bottom of the shell, and anti-slip pads are arranged at the bottoms of the supporting feet.

Compared with the prior art, the utility model has the following technical effects:

(1) According to the rotary shaking type automatic cell culture system, the liquid exchange in the culture dish is realized through the cooperation between the filter cartridge and the liquid exchange component, and the cells are separated from the waste liquid through the filter cartridge, so that the liquid exchange component is effectively prevented from sucking the cells, directly flushing the cells in the culture dish, and the success rate of cell culture is improved;

(2) According to the rotary shaking type automatic cell culture system, through the cooperation between the first screws and the fixed cylinder, culture dishes with different specifications are matched, and the adaptation degree of a culture assembly is improved;

(3) According to the rotary shaking type automatic cell culture system, the culture dish is shaken by matching the motor with the springs, so that liquid in the culture dish is shaken;

(4) According to the rotary shaking type automatic cell culture system, the shaking amplitude of the culture dish is adjusted through the cooperation between the adjusting bolt and the rotating shaft.

Drawings

FIG. 1 is a schematic perspective view of a rotary shaking type automatic cell culture system according to the present utility model;

FIG. 2 is a schematic view showing a partial sectional structure of a rotary shaking type automatic cell culture system according to the present utility model;

FIG. 3 is a schematic view showing a partial structure of a rotary shaking type automatic cell culture system according to the present utility model;

FIG. 4 is a schematic top view of the housing of the rotary shaking type automatic cell culture system according to the present utility model;

FIG. 5 is a schematic diagram showing the structure of a shaking unit in a rotary shaking type automatic cell culture system according to the present utility model;

FIG. 6 is a schematic view showing a partial cross-sectional structure of a shaking unit and a culturing unit in a rotary shaking automatic cell culturing system according to the present utility model;

FIG. 7 is a schematic diagram showing the structure of a housing assembly and a liquid exchange assembly in a rotary shaking type automatic cell culture system according to the present utility model.

Wherein the reference numerals are as follows:

10. a housing assembly; 11. a housing; 12. sealing cover; 13. an adjustment port; 14. an adjusting door;

20. shaking the assembly; 21. a motor; 22. a coupling; 23. a rotating shaft; 24. a shaking table; 25. a spring; 26. an adjusting bolt;

30. a culturing assembly; 31. fixing the cylinder; 32. a first screw; 33. a culture dish; 34. a filter cartridge; 35. a second screw;

40. a liquid exchange component; 41. a liquid taking straw; 42. a liquid exchange straw; 43. a waste liquid tank; 44. a culture solution tank; 45. and (5) supporting frames.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other.

The utility model is further described below with reference to the drawings and specific examples, which are not intended to be limiting.

A rotary shaking type automatic cell culture system, as shown in fig. 1-4, comprises a shell assembly 10, and a shaking assembly 20, a culture assembly 30 and a liquid exchange assembly 40 which are sequentially arranged in the inner cavity of the shell assembly 10 from bottom to top.

Wherein, the shell assembly 10 comprises a shell 11 and a sealing cover 12 which is detachably arranged at the top end of the shell 11; the shaking assembly 20 comprises a motor 21 arranged at the bottom of the inner cavity of the shell 11, the output end of the motor 21 is connected with a rotating shaft 23 through a coupler 22, a shaking table 24 is sleeved at the top end of the rotating shaft 23, and the outer edge of the middle upper part of the shaking table 24 is connected with the bottom of the inner cavity of the shell 11 through a plurality of springs 25 arranged at intervals; the culture assembly 30 comprises a fixed cylinder 31 arranged at the center of the top of the shaking table 24, the fixed cylinder 31 detachably sets a culture dish 33 in the inner cavity of the fixed cylinder 31 through a plurality of first screws 32 which are assembled with the side walls of the fixed cylinder 31 in a threaded manner, and a filter cartridge 34 is detachably arranged in the culture dish 33; the liquid exchange assembly 40 includes a liquid taking suction pipe 41 and a liquid exchange suction pipe 42 which are disposed above the filter cartridge 34, the liquid taking suction pipe 41 is connected with a waste liquid tank 43 through a hose, and the liquid exchange suction pipe 42 is connected with a culture liquid tank 44 through a hose.

Specifically, when shaking is required, the motor 21 is started to enable the rotating shaft 23 to drive the shaking table 24 to move, and the shaking table 24 drives the culture dish 33 to shake under the assistance of the springs 25, so that the shaking of the liquid in the culture dish 33 is realized. When the liquid needs to be changed, the liquid taking suction pipe 41 is arranged in the inner cavity of the filter cartridge 34, and the waste liquid in the culture dish 33 sequentially flows to the inner cavity of the filter cartridge 34, the liquid taking suction pipe 41 and the hose and is discharged to the waste liquid tank 43; the liquid-taking suction pipe 41 is taken out from the inner cavity of the filter cartridge 34, the liquid-changing suction pipe 42 is arranged in the inner cavity of the filter cartridge 34, and the culture liquid in the culture liquid box 44 sequentially flows to the hose, the liquid-changing suction pipe 42 and the inner cavity of the filter cartridge 34 and is discharged into the culture dish 33.

Wherein, through the cooperation between the motor 21 and the plurality of springs 25, the culture dish 33 is rocked, so as to realize the rocking of the liquid in the culture dish 33; through the cooperation between the filter cartridge 34 and the liquid exchange component 40, the liquid exchange in the culture dish 33 is realized, the liquid exchange component 40 is effectively prevented from directly flushing cells in the culture dish 33, and the success rate of cell culture is improved; through the cooperation between a plurality of first screw rods 32 and the fixed cylinder 31 to match the culture dish 33 of different specifications, improve the adaptation degree of cultivateing the subassembly 30.

In some embodiments, a water supply pump is arranged in the culture solution tank 44 and is connected with the liquid exchange straw 42 through a hose so as to realize discharge of the culture solution; a suction pump is provided in the waste liquid tank 43 and connected to the liquid suction pipe 41 via a hose to extract waste liquid.

In some embodiments, a control panel is disposed outside the casing 11 and is electrically connected to the motor 21, the water supply pump, and the water suction pump, respectively, so as to control the shaking assembly 20 to shake and the liquid exchange assembly 40 to exchange liquid.

In some of these embodiments, the sealing cap 12 is made of a transparent material in order to allow observation of the condition inside the culture dish 33.

In some of these embodiments, the top of the sealing cap 12 is provided with a convex lens to more intuitively observe the condition within the culture dish 33.

In some of these embodiments, the cells are placed in a culture dish 33 and outside of the filter cartridge 34; the bottom end of the filter cartridge 34 is provided with a filter screen so that cells in the culture dish 33 cannot enter the inner cavity of the filter cartridge 34, and liquid in the culture dish 33 can flow to the inner cavity of the filter cartridge 34. The cell in the culture dish 33 is effectively separated from the culture solution when the solution exchanging component 40 exchanges the solution.

Further, as shown in fig. 5 to 6, a connecting tube is provided at the bottom end of the shaking table 24, the connecting tube is sleeved outside the top end of the rotating shaft 23, and a plurality of springs 25 are provided at the middle upper part of the shaking table 24 in a ring shape at equal intervals.

In some embodiments, the shaking table 24 sequentially comprises a supporting table and a connecting cylinder from top to bottom, the connecting cylinder is vertically arranged at the bottom of the supporting table, and the springs 25 are annularly and equidistantly arranged at the bottom of the supporting table.

In some of these embodiments, a gap is left between the inner side wall of the connecting cylinder and the outer side wall of the upper end of the rotating shaft 23, so that the shaking table 24 can shake.

In some of these embodiments, the top end outer ring of the connecting cylinder is provided with a connecting flange, which is arranged at the bottom of the supporting table by a fixing bolt.

In some of these embodiments, the shaking table 24 comprises a support table and a connecting cylinder from top to bottom, and the connecting cylinder is vertically disposed at the bottom of the support table.

In some embodiments, the supporting table is a circular supporting table, the springs 25 are annularly and equally spaced at the bottom of the supporting table, and the springs 25 are located outside the motor 21.

In some embodiments, the support table is a square support table, and the springs 25 are respectively disposed at four corners of the bottom of the support table.

In some of these embodiments, four springs 25 are provided at intervals at the bottom of the support table.

Further, in order to adjust the shaking amplitude of the shaking table 24, the coupling 22 is detachably connected with the rotating shaft 23 through an adjusting bolt 26; an adjusting bolt 26 passes through the bottom end of the rotation shaft 23 and is screwed with the top end of the coupling 22.

In some embodiments, the adjusting bolt 26 is detached from the coupling 22, the angle between the bottom end of the rotating shaft 23 and the top end of the coupling 22 is adjusted, the adjusting bolt 26 is connected to the coupling 22, and the adjusting bolt 26 is tightened to fix the rotating shaft 23 to the coupling 22.

In some of these embodiments, in order to prevent relative rotation between the rotation shaft 23 and the overcoupling 22 after tightening the adjustment bolt 26, a locking washer is provided between the adjustment bolt 26 and the rotation shaft 23 to increase the friction between the joint bolt 26 and the rotation shaft 23.

Further, as shown in fig. 1 and 3, in order to facilitate adjusting the adjusting bolt 26, an adjusting port 13 is formed in a middle lower portion of the housing 11, a reversible adjusting door 14 is hinged to one side of the adjusting port 13, and the adjusting port 13 is disposed opposite to the adjusting bolt 26.

Further, as shown in fig. 4, the fixed cylinder 31 is disposed coaxially with the rotation shaft 23; the first screws 32 are annularly and equidistantly arranged on the side wall of the fixed cylinder 31, and the abutting ends of the first screws are sleeved with rubber sleeves.

In some of these embodiments, the tight end of the first screw 32 is sleeved with a rubber sleeve in order to effectively protect the outer wall of the culture dish 33.

In some embodiments, the number of first screws 32 is 3, and the first screws are annularly and equally spaced on the side wall of the fixed cylinder 31, so as to effectively fix the culture dish 33 to the inner cavity of the fixed cylinder 31. The culture dishes 33 with different specifications are matched through the threaded connection between the first screw rod 32 and the fixed cylinder 31.

Further, as shown in fig. 6, a plurality of second screws 35 are screw-fitted on the upper middle side wall of the filter cartridge 34, and the abutting ends of the second screws 35 are located outside the filter cartridge 34 and abut against the inner wall of the culture dish 33.

In some of these embodiments, the filter cartridge 34 is effectively secured to the interior cavity of the culture dish 33 by a threaded fit between the second screw 35 and the filter cartridge 34 to match different gauges of culture dishes 33.

In some embodiments, the number of second screws 35 is 3, and the second screws are annularly and equally spaced on the middle upper side wall of the filter cartridge 34.

In some of these embodiments, the second screws 35 are identical in number to the first screws 32 and are staggered on the side walls of the culture dish 33.

In some of these embodiments, the tight end of the second screw 35 is jacketed with a rubber jacket in order to effectively protect the inner wall of the culture dish 33.

Further, as shown in fig. 2 to 4 and 7, the liquid taking pipette 41 and the liquid changing pipette 42 are symmetrically arranged and fixed by the corresponding supporting frame 45.

In some embodiments, the filter cartridge 34 is coaxially arranged with the culture dish 33, and the liquid taking suction pipe 41 and the liquid changing suction pipe 42 are symmetrically arranged above the filter cartridge 34, so that later liquid changing is facilitated.

Further, as shown in fig. 2, the supporting frame 45 is 7-shaped, and its bottom end is fixed on the housing 11, and its lower end is an electric telescopic rod, so as to drive the liquid taking straw 41 or the liquid changing straw 42 at the top to move up and down.

Specifically, the liquid taking suction pipe 41 or the liquid changing suction pipe 42 is controlled to move up and down by the corresponding electric telescopic rod.

In some embodiments, a control panel is disposed outside the housing 11 and electrically connected to the motor 21, the water supply pump, the water pump, and the electric telescopic rod to control the liquid changing assembly 40 to change liquid.

Further, as shown in fig. 4 and 7, a waste liquid tank 43 and a culture liquid tank 44 are provided at the lower end of the outer wall of the housing 11, respectively.

In some of these embodiments, the incubator 44 houses a heating device that adjusts the temperature of the fluid in its lumen to match the temperature-sensitive cells.

Further, as shown in fig. 1 to 3 and 7, in order to prevent the housing 11 from slipping during use, support feet are provided at four corners of the bottom of the housing 11, and an anti-slip pad is provided at the bottom of the support foot.

The application method of the utility model is as follows:

before use, the filter cartridge 34 is placed in the inner cavity of the culture dish 33, and the filter cartridge 34 is fixed in the inner cavity of the culture dish 33 through a plurality of second screws 35;

placing the culture dish 33 in the inner cavity of the fixed cylinder 31, and fixing the culture dish 33 on the fixed cylinder 31 through a plurality of first screws 32;

placing the culture solution and the cells in the inner cavity of the culture dish 33, and the cells are positioned outside the filter cartridge 34;

the sealing cover 12 is detachably attached to the top of the housing 11.

When shaking, the motor 21 is started;

the coupler 22 drives the rotating shaft 23 to rotate, and the rotating shaft 23 drives the shaking table 24 to move;

with the aid of the springs 25, the shaking table 24 drives the culture dish 33 to shake, so that liquid in the culture dish 33 shakes.

When the liquid is changed, the liquid taking suction pipe 41 is controlled to move downwards through the corresponding supporting frame 45, and the bottom end of the liquid taking suction pipe 41 is arranged at the bottom of the inner cavity of the filter cylinder 34;

starting a water suction pump in the waste liquid tank 43 to enable the waste liquid in the culture dish 33 to sequentially flow to the inner cavity of the filter cartridge 34, the liquid taking suction pipe 41 and the hose, and then discharging the waste liquid to the waste liquid tank 43;

the liquid taking suction pipe 41 is controlled to move upwards through the corresponding supporting frame 45 so that the liquid taking suction pipe 41 is completely separated from the inner cavity of the filter cartridge 34;

the liquid exchange suction pipe 42 is controlled to move downwards through the corresponding supporting frame 45, and the bottom end of the liquid exchange suction pipe 42 is arranged at the bottom of the inner cavity of the filter cylinder 34;

starting a water supply pump in the culture solution tank 44 to enable the culture solution in the culture solution tank 44 to sequentially flow to the hose, the liquid exchange straw 42 and the inner cavity of the filter cartridge 34 and then to be discharged into the culture dish 33;

the liquid changing suction tube 42 is controlled to move upwards through the corresponding supporting frame 45, and the liquid changing suction tube 42 is completely separated from the inner cavity of the filter cartridge 34.

The foregoing description is only illustrative of the preferred embodiments of the present utility model and is not to be construed as limiting the scope of the utility model, and it will be appreciated by those skilled in the art that equivalent substitutions and obvious variations may be made using the description and illustrations of the present utility model, and are intended to be included within the scope of the present utility model.

Claims (10)

1. The utility model provides a rotatory automatic culture system that rocks formula cell which characterized in that, include casing subassembly (10) and from bottom to top set gradually in rocking subassembly (20), cultivation subassembly (30) and trading liquid subassembly (40) of casing subassembly (10) inner chamber, wherein:

the shell assembly (10) comprises a shell (11) and a sealing cover (12) detachably arranged at the top end of the shell (11);

the shaking assembly (20) comprises a motor (21) arranged at the bottom of the inner cavity of the shell (11), the output end of the motor (21) is connected with a rotating shaft (23) through a coupler (22), a shaking table (24) is sleeved at the top end of the rotating shaft (23), and the outer edge of the middle upper part of the shaking table (24) is connected with the bottom of the inner cavity of the shell (11) through a plurality of springs (25) arranged at intervals;

the culture assembly (30) comprises a fixed cylinder body (31) arranged at the center of the top of the shaking table (24), the fixed cylinder body (31) is used for detachably arranging a culture dish (33) in the inner cavity of the fixed cylinder body (31) through a plurality of first screw rods (32) which are in threaded assembly with the side walls of the fixed cylinder body, and a filter cylinder (34) is detachably arranged in the culture dish (33);

the liquid exchange assembly (40) comprises a liquid taking suction pipe (41) and a liquid exchange suction pipe (42) which are arranged above the filter cylinder (34), the liquid taking suction pipe (41) is connected with a waste liquid tank (43) through a hose, and the liquid exchange suction pipe (42) is connected with a culture liquid tank (44) through a hose.

2. The rotary shaking type automatic cell culture system according to claim 1, wherein a connecting cylinder is arranged at the bottom end of the shaking table (24), the connecting cylinder is sleeved outside the top end of the rotating shaft (23), and a plurality of springs (25) are annularly and equidistantly arranged at the middle upper part of the shaking table (24).

3. The rotary shaking type automatic cell culture system according to claim 1, wherein the coupling (22) is detachably connected with the rotation shaft (23) through an adjusting bolt (26); the adjusting bolt (26) passes through the bottom end of the rotating shaft (23) and is in threaded connection with the top end of the coupling (22).

4. The rotary shaking type automatic cell culture system according to claim 3, wherein an adjusting port (13) is formed in the middle lower portion of the shell (11), a turnover adjusting door (14) is hinged to one side of the adjusting port (13), and the adjusting port (13) and the adjusting bolt (26) are oppositely arranged.

5. The rotary shaking type automatic cell culture system according to claim 1, wherein the fixed cylinder (31) is coaxially disposed with the rotation shaft (23); the first screws (32) are annularly and equidistantly arranged on the side wall of the fixed cylinder (31), and the abutting ends of the first screws are sleeved with rubber sleeves.

6. The rotary shaking type automatic cell culture system according to claim 1, wherein a plurality of second screws (35) are threadedly assembled on the upper middle side wall of the filter cartridge (34), and the abutting ends of the second screws (35) are positioned outside the filter cartridge (34) and abut against the inner wall of the culture dish (33).

7. The rotary shaking type automatic cell culture system according to claim 1, wherein the liquid taking suction pipes (41) and the liquid changing suction pipes (42) are symmetrically arranged and are respectively fixed through corresponding supporting frames (45).

8. The automatic rotary shaking type cell culture system according to claim 7, wherein the supporting frame (45) has a 7-shaped structure, the bottom end of the supporting frame is fixed on the shell (11), and the lower end of the supporting frame is an electric telescopic rod so as to drive the liquid taking suction pipe (41) or the liquid exchanging suction pipe (42) at the top of the supporting frame to move up and down.

9. The rotary shaking type automatic cell culture system according to claim 1, wherein the waste liquid tank (43) and the culture liquid tank (44) are respectively provided at the lower end of the outer wall of the housing (11).

10. The rotary shaking type automatic cell culture system according to claim 1, wherein supporting feet are provided at four corners of the bottom of the housing (11), and an anti-slip pad is provided at the bottom of the supporting feet.