CN220181425U - Sample feeding device for tissue and blood DNA extraction - Google Patents
Sample feeding device for tissue and blood DNA extraction Download PDFInfo
- Publication number
- CN220181425U CN220181425U CN202320685026.9U CN202320685026U CN220181425U CN 220181425 U CN220181425 U CN 220181425U CN 202320685026 U CN202320685026 U CN 202320685026U CN 220181425 U CN220181425 U CN 220181425U
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- China
- Prior art keywords
- barrel
- wall
- tissue
- dna extraction
- cylinder body
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- 210000004369 blood Anatomy 0.000 title claims abstract description 17
- 239000008280 blood Substances 0.000 title claims abstract description 17
- 238000007400 DNA extraction Methods 0.000 title claims abstract description 13
- 238000004321 preservation Methods 0.000 claims description 24
- 244000309464 bull Species 0.000 claims description 13
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 7
- 229910052782 aluminium Inorganic materials 0.000 claims description 7
- 239000011888 foil Substances 0.000 claims description 7
- 125000003003 spiro group Chemical group 0.000 claims description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 2
- 235000017491 Bambusa tulda Nutrition 0.000 claims 2
- 241001330002 Bambuseae Species 0.000 claims 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 2
- 239000011425 bamboo Substances 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 7
- 241000894006 Bacteria Species 0.000 abstract description 5
- 229920000742 Cotton Polymers 0.000 abstract description 5
- 230000002779 inactivation Effects 0.000 abstract description 4
- 238000007789 sealing Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000001816 cooling Methods 0.000 abstract description 3
- 238000000354 decomposition reaction Methods 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 239000011248 coating agent Substances 0.000 abstract description 2
- 238000000576 coating method Methods 0.000 abstract description 2
- 238000009413 insulation Methods 0.000 abstract 3
- 238000002844 melting Methods 0.000 abstract 1
- 230000008018 melting Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 16
- 108020004414 DNA Proteins 0.000 description 12
- 238000005070 sampling Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000005192 partition Methods 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a sample feeding device for tissue and blood DNA extraction, which comprises a cylinder body, wherein the top end of the cylinder body is in threaded connection with a top cover, the bottom end of the cylinder body is inserted with a bottom cover, and the inner wall of the cylinder body is provided with an insulating layer. Preparing proper amount of ice cubes, placing the ice cubes in the bottom cover, inserting the bottom cover into the barrel, locating below the barrel, cooling the ice cubes, coating the surface of the heat insulation cotton with heat insulation layer, protecting the heat insulation cotton, avoiding the ice cubes from melting rapidly in the transportation process, prolonging the use of the ice cubes, ensuring proper temperature in the sample feeding process, avoiding DNA decomposition, degradation, inactivation and pollution, ensuring good tightness for the twice sealing effect, preventing external harmful chemical substances, bacteria and microorganisms from entering and damaging the structure and function of DNA molecules, and sterilizing the barrel to prevent the sample from being polluted and deactivated.
Description
Technical Field
The utility model relates to the technical field of blood sample storage, in particular to a sample feeding device for tissue and blood DNA extraction.
Background
Tissue and blood DNA extraction is a commonly used biological assay for extracting and purifying DNA from organisms for subsequent molecular biological research and application. For tissue samples, it is often necessary to first perform cell lysis and tissue disruption treatments to release intracellular DNA. For blood samples, an anticoagulant is usually added, and then the white blood cells are separated by centrifugation, followed by cell lysis. Subsequently, the DNA is extracted using chemical or physical methods such as chloroform, isopropanol precipitation, or phenol/chloroform extraction, etc. Finally, detecting the quality and concentration of the DNA by electrophoresis or colorimetry, and ensuring the high quality and enough concentration of the DNA so as to facilitate the smooth proceeding of the subsequent operation. Tissue and blood DNA extraction can be widely applied to molecular biology experiments such as genome sequencing, PCR amplification, molecular cloning, gene expression and the like, proper temperature needs to be ensured in the sample feeding process, the conditions of DNA decomposition, degradation, inactivation, pollution and the like can be caused by overhigh temperature, the sealing performance of the existing preservation container is poor, external harmful chemical substances, bacteria and microorganisms enter and destroy the structure and the function of DNA molecules, sterilization treatment is not performed, DNA samples are easy to pollute and inactivate, related information cannot be accurately acquired from samples, and the practicability is poor.
Disclosure of Invention
The utility model aims to provide a sample feeding device for tissue and blood DNA extraction, which solves the problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a sample presentation device of tissue and blood DNA extraction, includes the barrel, the top spiro union of barrel has the top cap, the bottom of barrel has pegged graft the bottom, the inner wall of barrel is provided with the heat preservation, the inner wall central point of top cap puts and rotates the dress and have the bull stick, the fixed baffle that has cup jointed of outer wall of bull stick, the rubber circle that has cup jointed of the side of baffle is fixed, roof and layer board have been cup jointed in proper order to the outer wall of bull stick from top to bottom, the opening has been seted up on the surface of roof, open-ended quantity is a plurality of, the bottom fixed assembly of bull stick has the bottom plate, the side of bottom plate is fixed to cup joint the sponge circle, the fixed ultraviolet lamp board that is equipped with in bottom surface of bottom plate.
Preferably, the sliding grooves are formed in two sides of the surface of the bottom plate, the sliding blocks are connected to the inner walls of the sliding grooves in a sliding and clamping mode, and the supporting rods are fixedly assembled on the surfaces of the sliding blocks.
Preferably, the bottom surface of the supporting rod is provided with a notch.
Preferably, the inner wall of the cylinder is fixedly provided with a frame, and the inner wall of the frame is fixedly provided with an aluminum foil film.
Preferably, the bottom surface of the bottom cover is fixedly provided with a rubber cylinder, and the bottom end of the cylinder body is positioned in the rubber cylinder.
Preferably, the side surface of the cylinder is attached with label paper.
Compared with the prior art, the utility model has the beneficial effects that: a sample feeder for extracting tissue and blood DNA features that when the sampled test tube is stored, it is inserted in the opening of top plate, the test tube is stored and supported on the surface of supporting plate, the test tube can be stably placed, the ultraviolet lamp plate is used to irradiate the cylinder, the bottom plate is extended to the cylinder, a proper amount of disinfecting alcohol is dripped on the sponge ring, the sponge block wipes the cylinder along with the movement of bottom plate for further disinfecting, preventing bacteria from remaining in cylinder, the supporting plate, test tube and top plate are moved into cylinder, and the partition plate is attached to the inner surface of cylinder, the utility model has the advantages of play sealed effect, avoid entering of dust and bacteria, rotate the top cap and screw together top cap and barrel, then prepare right amount of ice-cubes, further seal, improve sealed effect, place the ice-cubes in the inside of bottom, the bottom can insert in the barrel, be located in the lower part of barrel, the ice-cubes can play the effect of cooling down, the heat preservation is the heat preservation cotton, the surface of heat preservation cotton is coated with the coating, protect the heat preservation cotton, play the heat preservation effect, avoid the ice-cubes to melt fast in the transportation process, prolong the use of ice-cubes, can ensure temperature fit in the sample feeding process, avoid conditions such as DNA decomposition, degradation, inactivation and pollution, ensure good sealing performance for two times, prevent outside harmful chemical substances, bacteria and microorganism from entering and destroying the structure and function of DNA molecule, meanwhile, the inside of the cylinder body is also disinfected, so that the pollution and inactivation of the sample are prevented.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model.
FIG. 2 is a cross-sectional view of the cap and rubber barrel construction of the present utility model.
Fig. 3 is a cross-sectional view of the structure of the present utility model.
Fig. 4 is a top view of the top plate structure of the present utility model.
In the figure: 1. a cylinder; 2. a top cover; 3. a bottom cover; 4. a rubber cylinder; 5. a heat preservation layer; 6. a rotating rod; 7. a partition plate; 8. a rubber ring; 9. a top plate; 10. a supporting plate; 11. a frame; 12. an aluminum foil film; 13. an opening; 14. a bottom plate; 15. a sponge ring; 16. an ultraviolet lamp plate; 17. a chute; 18. a slide block; 19. a support rod; 20. a notch; 21. and (3) label paper.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-4, the present utility model provides a technical solution: the utility model provides a sample device that tissue and blood DNA extracted, including barrel 1, the top spiro union of barrel 1 has top cap 2, bottom 3 has been pegged graft to the bottom of barrel 1, the inner wall of barrel 1 is provided with heat preservation 5, the inner wall central point of top cap 2 puts and rotates the assembly and have bull stick 6, the fixed baffle 7 that has cup jointed of outer wall of bull stick 6, the fixed rubber circle 8 that has cup jointed of side of baffle 7, roof 9 and layer board 10 have been cup jointed to the outer wall of bull stick 6 from top to bottom in proper order, opening 13 has been seted up on the surface of roof 9, the quantity of opening 13 is a plurality of, the bottom fixed assembly of bull stick 6 has bottom plate 14, the fixed sponge circle 15 that has cup jointed of side of bottom plate 14, the fixed ultraviolet lamp board 16 that is equipped with in bottom surface of bottom plate 14.
When depositing the test tube after taking a sample, insert the test tube in the opening 13 on roof 9, the below of test tube is located on the surface of layer board 10, the size of opening 13 and the quantity of opening 13 need be set for according to the size of the test tube of in-service use, use ultraviolet lamp board 16 to shine the inside of barrel 1, ultraviolet lamp board 16 needs cooperation battery and switch to use, specific use mode is prior art, do not do too much in this, stretch into the inside of barrel 1 with bottom plate 14, stretch into the disinfection alcohol of proper amount on the sponge circle 15 before, along with the removal of bottom plate 14, the sponge piece is cleaned the inside of barrel 1, carry out further disinfection treatment, along with the inside that the bull stick 6 stretches into barrel 1, layer board 10, test tube and roof 9 enter into the inside of barrel 1 together, then baffle 7 enters into the inside of barrel 1, the inner wall laminating of rubber circle 8 and barrel 1 together this moment, play sealed effect until top cap 2 is located 1 top, rotate top cap 2 and 1 spiro union together, afterwards stretch into the inside of barrel 3 with bottom plate, can be in the heat preservation brush 3, can be in the heat preservation position of the inside of barrel 1, can be in the heat preservation brush, can be in the heat preservation layer is stretched into, the heat preservation layer is in the heat preservation, can be in the heat preservation layer is in the heat preservation, can's the heat preservation is in the inside of the heat preservation layer is in the heat preservation, can be in the heat preservation, and the inside is in the heat preservation layer, and can be in the heat preservation, and the inside.
Specifically, both sides of the surface of the bottom plate 14 are provided with sliding grooves 17, the inner walls of the sliding grooves 17 are slidably clamped with sliding blocks 18, and the surface of the sliding blocks 18 is fixedly provided with supporting rods 19.
In the process of sampling, the supporting rod 19 is pulled, the sliding blocks 18 on the supporting rod 19 can move in the sliding grooves 17 on the surface of the bottom plate 14, the supporting rods 19 on two sides are pulled to two sides, the supporting rods 19 are directly placed at the top edge position of the cylinder body 1, the ultraviolet lamp plate 16 is opened at the moment to sterilize the inside of the cylinder body 1, the test tube support can be inserted into the opening 13 after sampling, the bottom end of the test tube is placed on the supporting plate 10, the supporting rods 19 are moved to the original position after sampling is completed, the rotating rods 6 extend into the cylinder body 1, the test tube is placed in the cylinder body 1, and the sampling and sterilizing processes can be simultaneously carried out, so that the efficiency is improved.
Specifically, the bottom surface of the strut 19 is provided with a notch 20.
The bottom surface at branch 19 sets up a breach 20, and the width of breach 20 is greater than the wall thickness of barrel 1, and after pulling branch 19, can be with the breach 20 joint on the border of barrel 1, on the branch 19 joint of both sides barrel 1, can support the top at barrel 1, more stable.
Specifically, the inner wall of the cylinder 1 is fixedly equipped with a frame 11, and the inner wall of the frame 11 is fixedly equipped with an aluminum foil film 12.
The inner wall of the barrel body 1 is provided with the frame 11, the frame 11 is used for fixing the aluminum foil film 12, the aluminum foil film 12 is used for separating ice cubes in the bottom cover 3, the pollution to the reagent tube is avoided, the heat conducting performance of the aluminum foil film 12 is good, and the cooling effect of the ice cubes cannot be affected.
Specifically, the bottom surface of the bottom cover 3 is fixedly provided with a rubber cylinder 4, and the bottom end of the cylinder body 1 is positioned in the rubber cylinder 4.
The rubber cylinder 4 is arranged on the bottom surface of the bottom cover 3, so that the friction force between the rubber cylinder and a tabletop can be improved when the rubber cylinder is placed, the stability of the cylinder 1 is improved, the bottom end of the cylinder 1 is prevented from being knocked, and the bottom cover 3 is protected.
Specifically, the label paper 21 is attached to the side surface of the cylinder 1.
A label paper 21 is stuck on the surface of the cylinder 1, so that the information can be recorded while sampling is facilitated.
Working principle: when the sampled test tube is stored, the test tube is inserted into the opening 13 on the top plate 9, the lower side of the test tube is positioned on the surface of the supporting plate 10, the ultraviolet lamp plate 16 is used for irradiating the inside of the cylinder 1, the bottom plate 14 is extended into the inside of the cylinder 1, a proper amount of disinfection alcohol is dripped on the sponge ring 15 before the bottom plate is extended, the sponge block wipes the inside of the cylinder 1 along with the movement of the bottom plate 14, further disinfection treatment is carried out, the supporting plate 10, the test tube and the top plate 9 are together introduced into the inside of the cylinder 1 along with the extension of the rotating rod 6 into the inside of the cylinder 1, then the partition 7 is introduced into the inside of the cylinder 1, at the moment, the rubber ring 8 and the inner wall of the cylinder 1 are attached together to play a sealing role until the top cover 2 is positioned above the cylinder 1, the top cover 2 is rotated to screw the top cover 2 and the cylinder 1 together, then a proper amount of ice cubes are prepared, the ice cubes are placed inside the bottom cover 3, and the bottom cover 3 can be inserted into the inside of the cylinder 1 at a position below the cylinder 1.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A sample presentation device for tissue and blood DNA extraction, characterized in that: including barrel (1), the top spiro union of barrel (1) has top cap (2), bottom (3) have been pegged graft to the bottom of barrel (1), the inner wall of barrel (1) is provided with heat preservation (5), the inner wall central point of top cap (2) puts and rotates and be equipped with bull stick (6), baffle (7) have been cup jointed to the outer wall of bull stick (6), rubber circle (8) have been cup jointed to the side of baffle (7) are fixed, roof (9) and layer board (10) have been cup jointed by fixed in proper order from top to bottom to the outer wall of bull stick (6), opening (13) have been seted up on the surface of roof (9), the quantity of opening (13) is a plurality of, the bottom fixed bottom plate (14) that is equipped with of bull stick (6), sponge circle (15) have been cup jointed to the side of bottom plate (14) are fixed to be equipped with ultraviolet lamp board (16), spout (17) have all been seted up to the surface both sides of bottom plate (14), the inner wall slip joint of spout (17) has slider (18), the fixed surface of branch (19) is equipped with slider (18).
2. The sample presentation device for tissue and blood DNA extraction of claim 1, wherein: the bottom surface of branch (19) is opened and is opened opening (20).
3. The sample presentation device for tissue and blood DNA extraction of claim 1, wherein: the inner wall of the cylinder body (1) is fixedly provided with a frame (11), and the inner wall of the frame (11) is fixedly provided with an aluminum foil film (12).
4. The sample presentation device for tissue and blood DNA extraction of claim 1, wherein: the bottom surface of bottom (3) is equipped with rubber section of thick bamboo (4) fixedly, the bottom of barrel (1) is located in rubber section of thick bamboo (4).
5. The sample presentation device for tissue and blood DNA extraction of claim 1, wherein: the side surface of the cylinder body (1) is adhered with label paper (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320685026.9U CN220181425U (en) | 2023-03-31 | 2023-03-31 | Sample feeding device for tissue and blood DNA extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320685026.9U CN220181425U (en) | 2023-03-31 | 2023-03-31 | Sample feeding device for tissue and blood DNA extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220181425U true CN220181425U (en) | 2023-12-15 |
Family
ID=89106121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320685026.9U Active CN220181425U (en) | 2023-03-31 | 2023-03-31 | Sample feeding device for tissue and blood DNA extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220181425U (en) |
-
2023
- 2023-03-31 CN CN202320685026.9U patent/CN220181425U/en active Active
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