CN220099045U - Cell incubator for monoclonal antibody preparation - Google Patents
Cell incubator for monoclonal antibody preparation Download PDFInfo
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- CN220099045U CN220099045U CN202320839479.2U CN202320839479U CN220099045U CN 220099045 U CN220099045 U CN 220099045U CN 202320839479 U CN202320839479 U CN 202320839479U CN 220099045 U CN220099045 U CN 220099045U
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- water tank
- monoclonal antibody
- box
- cell culture
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- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 59
- 238000003860 storage Methods 0.000 claims abstract description 12
- 238000001125 extrusion Methods 0.000 claims description 16
- 230000007246 mechanism Effects 0.000 claims description 14
- 238000004113 cell culture Methods 0.000 claims description 12
- 230000016784 immunoglobulin production Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 abstract description 14
- 238000005192 partition Methods 0.000 abstract description 11
- 239000001963 growth medium Substances 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 11
- 238000000034 method Methods 0.000 description 4
- 210000004408 hybridoma Anatomy 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008076 immune mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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Abstract
The utility model discloses a cell incubator for monoclonal antibody preparation, which belongs to the technical field of monoclonal antibody preparation and comprises a box body, wherein a box door is hinged to one side of the box body, a sealing water tank is arranged at the inner bottom of the box body, a water storage tank is arranged at the outer side of the box body, and a guide pipe is arranged between the water storage tank and the sealing water tank. The first through hole at the top of the sealed water tank is arranged, so that the tank body can be communicated with the inside of the water tank, the ambient humidity in the tank body is ensured, in addition, the first spring, the partition board and the second through hole are arranged in the transverse sliding groove, the first through hole and the second through hole can be automatically misplaced after the box door is opened, the sealed water tank is plugged, and after the box door is closed, the first spring automatically pushes the partition board to conduct the first through hole and the second through hole, so that the pollution of the external environment to the water source in the sealed water tank is avoided, and the culture environment of cells is ensured.
Description
Technical Field
The utility model relates to a cell incubator, in particular to a cell incubator for monoclonal antibody preparation, and belongs to the technical field of monoclonal antibody preparation.
Background
Monoclonal antibodies are highly homogeneous antibodies raised by single B cell cloning and directed against only a specific epitope, and are usually prepared by hybridoma technology, and single hybridoma cells with such properties are cultured into cell populations, thereby fundamentally solving the problems of specificity and reproducibility which exist for a long time in antibody preparation, and being directly applicable to diagnosis, prevention, treatment and research of immune mechanism of diseases, while cell culture boxes are required in the preparation process of monoclonal antibodies.
The utility model of application number 202021641956.7 discloses a cell incubator for monoclonal antibody preparation, through setting up water supply assembly, set up a catch basin in the bottom of box, link to each other with the water tank through the water conduit, when the water level in the catch basin reaches certain height, buoyancy ball come up, the delivery port is plugged up to the plug on the connecting rod, can effectually avoid the frequent humidity condition of looking over the incubator of operating personnel, when the water level descends, water in the catch basin is automatic to be filled, through setting up fixed subassembly, place the culture medium in the circular recess of fixing base inner wall, through pulling the pull rod, can fix the culture medium, the culture medium produces to rock when avoiding the incubator to remove or carry, lead to the damage.
Similar to the above-mentioned application, there are currently disadvantages:
1. after the door of the box is opened, the water source inside the water tank is directly contacted with the external environment, so that the pollution of the water source is easily caused, and the culture of cells is influenced;
2. in the process of placing the culture medium, the pull rod is pulled firstly, the clamping plate is moved outwards, the culture medium can be extruded and positioned, and the mounting and the placing are inconvenient;
to this end, a cell incubator for monoclonal antibody production was designed to optimize the above problems.
Disclosure of Invention
The utility model mainly aims to provide a cell culture box for preparing monoclonal antibodies, which is characterized in that a first through hole is formed in the top of a sealed water tank, the box body can be communicated with the inside of the water tank, the environment humidity in the box body is ensured, in addition, the first through hole and the second through hole are automatically misplaced after a box door is opened through the arrangement of a first spring, a partition plate and a second through hole, the sealed water tank is plugged, the partition plate is automatically pushed by the first spring to conduct the first through hole and the second through hole after the box door is closed, the pollution of the external environment to a water source in the sealed water tank is avoided, the culture environment of cells is ensured, and the arrangement of a positioning mechanism consisting of a supporting plate top placing groove, a telescopic groove, a second spring, a squeezing block, a supporting plate, a third spring, a plug rod, a slot, a longitudinal sliding groove, a sliding block and an annular sliding groove is realized.
The aim of the utility model can be achieved by adopting the following technical scheme:
the utility model provides a cell incubator for monoclonal antibody preparation, includes the box, one side of box articulates there is the chamber door, the interior bottom of box is provided with sealed water tank, the outside of box is provided with the water storage tank, be provided with the pipe between water storage tank and the sealed water tank, horizontal spout has all been seted up to the both sides at sealed water tank top, first through-hole has all been seted up to the bottom of horizontal spout, the inside of horizontal spout all slides and is provided with the baffle, all seted up the second through-hole on the baffle, be provided with first spring between the tip of baffle and the tip of horizontal spout, the inside of box is provided with the backup pad, all be provided with positioning mechanism in the backup pad.
Preferably: the outside of water storage tank has seted up transparent window, and is provided with the liquid level line on the transparent window.
Preferably: limiting grooves are symmetrically formed in two sides of the inside of the box body, and the supporting plates are arranged between the limiting grooves in a sliding mode.
Preferably: the supporting plate is provided with two groups, and the positioning mechanisms at the top of the supporting plate are provided with two groups.
Preferably: the bottom outside the sealed water tank is provided with the drain valve, and the outside of sealed water tank is provided with the observation window.
Preferably: the positioning mechanism comprises a placing groove, a telescopic groove and a longitudinal sliding groove, wherein the placing groove is formed in the top of the supporting plate, the telescopic groove is uniformly formed in the outer side of the placing groove, a second spring is arranged at the inner end of the telescopic groove, an extrusion block is arranged at the end of the second spring, the top and the bottom of the extrusion block are both provided with oblique sides, a supporting plate is arranged between the extrusion blocks, a third spring is arranged between the supporting plate and the bottom of the placing groove, an inserting rod is fixed at the middle position of the bottom of the supporting plate, a slot is formed in the bottom of the placing groove, the longitudinal sliding groove is symmetrically formed in the two sides of the slot, a sliding block is slidably arranged in the longitudinal sliding groove, and the sliding block is fixedly connected with the inserting rod, and an annular sliding groove is formed in the bottom of the slot.
Preferably: the top of layer board is provided with the slipmat, and the antiskid line has been seted up at the top of slipmat.
The beneficial effects of the utility model are as follows:
according to the cell culture box for preparing the monoclonal antibody, the first through hole at the top of the sealing water box is arranged, so that the box body can be communicated with the interior of the water box, the internal environment humidity of the box body is ensured, in addition, the first through hole and the second through hole can be automatically misplaced after the box door is opened through the arrangement of the first spring, the partition plate and the second through hole in the transverse sliding groove, the sealing water box is blocked, and after the box door is closed, the first spring automatically pushes the partition plate to conduct the first through hole and the second through hole, the pollution of the external environment to the water source in the sealing water box is avoided, and the culture environment of cells is ensured;
the setting of the positioning mechanism that backup pad top standing groove, expansion groove, second spring, extrusion piece, layer board, third spring, inserted bar, slot, vertical spout, slider, annular spout are constituteed through placing the culture medium to the top of layer board to the pushing down of control layer board, then rotatory layer board stabilizes the height that the culture medium was placed, and utilizes extrusion piece to carry out automatic extrusion to the culture medium and fix, makes the putting of culture medium more convenient, and stability is higher.
Drawings
FIG. 1 is a view showing the construction of the inside of a case according to the present utility model;
FIG. 2 is a diagram of the sealing water tank of the present utility model;
FIG. 3 is a top view of the sealed water tank of the present utility model;
fig. 4 is a cross-sectional view of a support plate of the present utility model.
In the figure: 1. a case; 2. a door; 3. sealing the water tank; 4. a water storage tank; 5. a transverse chute; 6. a first port; 7. a first spring; 8. a partition plate; 9. a second port; 10. a support plate; 11. a positioning mechanism; 12. a placement groove; 13. a telescopic slot; 14. a second spring; 15. extruding a block; 16. a supporting plate; 17. a third spring; 18. a rod; 19. a slot; 20. a longitudinal chute; 21. a slide block; 22. an annular chute.
Detailed Description
In order to make the technical solution of the present utility model more clear and clear to those skilled in the art, the present utility model will be described in further detail with reference to examples and drawings, but the embodiments of the present utility model are not limited thereto.
Example 1
As shown in fig. 1-4, the embodiment provides a cell incubator for preparing monoclonal antibodies, which comprises a box body 1, one side of the box body 1 is hinged with a box door 2, the inner bottom of the box body 1 is provided with a sealing water tank 3, the bottom of the outer side of the sealing water tank 3 is provided with a drain valve, the outer side of the sealing water tank 3 is provided with an observation window, the outer side of the box body 1 is provided with a water storage tank 4, the outer side of the water storage tank 4 is provided with a transparent window, a liquid level line is arranged on the transparent window, a conduit is arranged between the water storage tank 4 and the sealing water tank 3, both sides of the top of the sealing water tank 3 are provided with transverse sliding grooves 5, the bottom of the transverse sliding grooves 5 are provided with first through openings 6, the inside of the transverse sliding grooves 5 are provided with a partition plate 8, both sides of the partition plate 8 are provided with second through openings 9, a first spring 7 is arranged between the end of the transverse sliding grooves 5, the inside of the box body 1 is provided with a supporting plate 10, both sides of the inner side of the box body 1 are symmetrically provided with limiting grooves, the supporting plate 10 are slidingly arranged between the limiting grooves, both sides of the supporting plate 10 are provided with positioning mechanisms 11, both sets of the supporting plate 10 are provided with positioning mechanisms, and both sets of the positioning mechanisms 11 are arranged.
When the refrigerator is used, a culture medium is placed on the top of the supporting plate 10, then the position of the culture medium is stabilized by the aid of the positioning mechanism 11, after the culture medium is placed, the refrigerator door 2 is closed, the partition plate 8 is extruded in the process of closing the refrigerator door 2, the second port 9 is aligned with the first port 6, the sealing water tank 3 is communicated with the inside of the refrigerator body 1, proper humidity is provided for the inside of the refrigerator body 1, after the refrigerator door 2 is opened, the partition plate 8 is pushed by the first spring 7 to reset, the first port 6 and the second port 9 are staggered, the sealing water tank 3 is thoroughly sealed, and water source pollution is avoided.
Example two
In this embodiment, as shown in fig. 1-4, the positioning mechanism 11 includes a placement groove 12, a telescopic groove 13 and a longitudinal sliding groove 20, the placement groove 12 is formed at the top of the support plate 10, the telescopic groove 13 is uniformly formed at the outer side of the placement groove 12, the second springs 14 are all installed at the inner ends of the telescopic groove 13, the extrusion blocks 15 are all installed at the ends of the second springs 14, oblique sides are all provided at the top and bottom of the extrusion blocks 15, a supporting plate 16 is arranged between the extrusion blocks 15, a third spring 17 is arranged between the supporting plate 16 and the bottom of the placement groove 12, an inserting rod 18 is fixed at the middle position of the bottom of the supporting plate 16, a slot 19 is formed at the bottom of the placement groove 12, longitudinal sliding grooves 20 are symmetrically formed at two sides of the slot 19, sliding blocks 21 are all slidably arranged in the longitudinal sliding grooves 20, the sliding blocks 21 are fixedly connected with the inserting rod 18, and an annular sliding groove 22 is formed at the bottom of the slot 19.
When the culture medium is placed, the culture medium is placed on the top of the supporting plate 16, then the downward movement of the supporting plate 16 is controlled, at the moment, the inserting rod 18 is inserted into the slot 19, the sliding block 21 on the inserting rod 18 slides in the longitudinal sliding groove 20 and slides into the annular sliding groove 22, then the sliding block 21 drives the rotation of the supporting plate 16 by utilizing the friction force between the culture medium and the supporting plate 16 through rotating the culture medium, the height of the inserting rod 18 and the supporting plate 16 is stabilized, in addition, in the downward movement process of the supporting plate 16, the supporting plate 16 is separated from the extrusion block 15, the second spring 14 controls the extrusion block 15 to be attached to the outer side of the culture medium for extrusion positioning, when the culture medium is taken out, the supporting plate 16 is rotated again, the sliding block 21 is rotated into the longitudinal sliding groove 20, the supporting plate 16 is moved upwards by the third spring 17 and the extrusion block 15 is extruded into the telescopic groove 13 in the upward movement process of the supporting plate 16, and the culture medium is taken out more conveniently.
In this embodiment, the top of the pallet 16 is provided with a skid-proof pad, and the top of the skid-proof pad is provided with skid-proof grains.
The friction between the culture medium and the pallet 16 during use is increased.
The above description is merely a further embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto, and any person skilled in the art will be able to apply equivalents and modifications according to the technical solution and the concept of the present utility model within the scope of the present utility model disclosed in the present utility model.
Claims (7)
1. A cell culture box for monoclonal antibody preparation, characterized in that: including box (1), one side of box (1) articulates there is chamber door (2), the interior bottom of box (1) is provided with sealed water tank (3), the outside of box (1) is provided with water storage tank (4), be provided with the pipe between water storage tank (4) and sealed water tank (3), horizontal spout (5) have all been seted up to the both sides at sealed water tank (3) top, first opening (6) have all been seted up to the bottom of horizontal spout (5), the inside of horizontal spout (5) all slides and is provided with baffle (8), all seted up second opening (9) on baffle (8), be provided with first spring (7) between the tip of baffle (8) and the tip of horizontal spout (5), the inside of box (1) is provided with backup pad (10), all be provided with positioning mechanism (11) on backup pad (10).
2. A cell culture incubator for monoclonal antibody production according to claim 1, wherein: the outside of water storage tank (4) has seted up transparent window, and is provided with the liquid level line on the transparent window.
3. A cell culture incubator for monoclonal antibody production according to claim 2, wherein: limiting grooves are symmetrically formed in two sides of the inside of the box body (1), and the supporting plate (10) is arranged between the limiting grooves in a sliding mode.
4. A cell culture incubator for monoclonal antibody production according to claim 3, wherein: the support plate (10) is provided with two groups, and the positioning mechanisms (11) at the top of the support plate (10) are provided with two groups.
5. The cell culture incubator of claim 4, wherein the cell culture incubator is used for monoclonal antibody preparation, and the cell culture incubator is characterized by: the bottom outside sealed water tank (3) is provided with the drain valve, and the outside of sealed water tank (3) is provided with the observation window.
6. A cell culture incubator for monoclonal antibody production according to claim 5, wherein: positioning mechanism (11) are including standing groove (12), expansion groove (13) and vertical spout (20), the top in backup pad (10) is seted up in standing groove (12), expansion groove (13) have evenly been seted up in the outside of standing groove (12), second spring (14) are all installed to the inner tip of expansion groove (13), extrusion piece (15) are all installed to the tip of second spring (14), the top and the bottom of extrusion piece (15) all are provided with the hypotenuse, be provided with layer board (16) between extrusion piece (15), be provided with third spring (17) between layer board (16) and the bottom of standing groove (12), intermediate position department of layer board (16) bottom is fixed with inserted bar (18), slot (19) have been seted up to the bottom of standing groove (12), vertical spout (20) have all been slided in the inside of longitudinal spout (20), slider (21) and inserted bar (18) fixed connection, annular spout (22) have been seted up to the bottom of slot (19).
7. A cell culture incubator for monoclonal antibody production according to claim 6, wherein: the top of the supporting plate (16) is provided with an anti-slip pad, and the top of the anti-slip pad is provided with anti-slip patterns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320839479.2U CN220099045U (en) | 2023-04-11 | 2023-04-11 | Cell incubator for monoclonal antibody preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320839479.2U CN220099045U (en) | 2023-04-11 | 2023-04-11 | Cell incubator for monoclonal antibody preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220099045U true CN220099045U (en) | 2023-11-28 |
Family
ID=88844180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320839479.2U Active CN220099045U (en) | 2023-04-11 | 2023-04-11 | Cell incubator for monoclonal antibody preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220099045U (en) |
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2023
- 2023-04-11 CN CN202320839479.2U patent/CN220099045U/en active Active
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