CN220618930U - Multilayer cell culture dish with drainage groove - Google Patents
Multilayer cell culture dish with drainage groove Download PDFInfo
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- CN220618930U CN220618930U CN202322137532.7U CN202322137532U CN220618930U CN 220618930 U CN220618930 U CN 220618930U CN 202322137532 U CN202322137532 U CN 202322137532U CN 220618930 U CN220618930 U CN 220618930U
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- cell culture
- dish
- bodies
- inner sides
- culture dish
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- 238000004113 cell culture Methods 0.000 title claims abstract description 45
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 125000003003 spiro group Chemical group 0.000 claims description 3
- 239000002699 waste material Substances 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 4
- 238000007599 discharging Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 6
- 230000006872 improvement Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a culture dish, which belongs to the technical field of cell culture, in particular to a multilayer cell culture dish with drainage grooves, comprising a plurality of dish bodies, wherein the upper sides of the dish bodies are all in threaded connection with sealing cover bodies, and the inner sides of the dish bodies are fixed with fixing plates; according to the utility model, a plurality of dish bodies are arranged, so that a plurality of cells can be cultured at the same time, the efficiency of cell culture is improved, the discharge of cell culture waste liquid is facilitated through the liquid discharge mechanism, the dish bodies can be sealed through the sealing plugs when the dish bodies are not used, the sealing plugs can be opened when new cell culture liquid needs to be placed, the new cell culture liquid is injected through the injector, and the influence on the cell culture quality due to the contact of the cells with external air in the culture process is avoided; the problem of current be inconvenient for discharging the waste liquid that the inside experiment of culture dish produced, unable to stack a plurality of culture dishes and place, occupation space is great is solved.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a multilayer cell culture dish with drainage grooves.
Background
A dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disk-shaped bottom and a lid, generally made of glass or plastic.
Through retrieving, chinese patent with publication No. CN215947283U discloses a culture dish, and it is including being lid and closing upper cover and the ware body that connects, and ware body and upper cover all are single open structure offer a plurality of ventilation holes on the lateral wall of upper cover be fixed with a plurality of supporting shoe on the ware body lateral wall, the inner wall shaping of upper cover has a plurality of lugs, the height of supporting shoe and lug equals, the ventilation hole is located the lug and is close to upper cover open-ended one side, supporting shoe and lug all encircle the center pin distribution of ware body.
The device can adjust the air inflow of the culture dish;
but this device also has the following problems:
the device can't discharge the waste liquid that culture dish inside experiment produced, is inconvenient for reducing cross infection between the different cells, and when cell culture, generally need change cell culture liquid, repeatedly open the lid and make outside bacterium get into in the culture dish easily, influence experimental effect, and can't stack a plurality of culture dishes and place, occupation space is great.
Disclosure of Invention
In order to solve the technical problems, the utility model provides a multilayer cell culture dish with a drainage groove, which is convenient for replacing waste liquid, does not need to repeatedly open a cover body to operate, and affects the quality of cell culture.
The technical solution for realizing the purpose of the utility model is as follows: the multilayer cell culture dish with the drainage groove comprises a plurality of dish bodies, wherein the upper sides of the dish bodies are all in threaded connection with sealing cover bodies, the inner sides of the dish bodies are fixedly provided with fixing plates, and the inner sides of the fixing plates are provided with sliding grooves at one end position; the liquid draining mechanisms are respectively arranged above the plurality of fixing plates; the liquid draining mechanism comprises a fixed box fixed on the outer side of a plurality of dishes and a liquid draining pipe arranged on one side of the dishes, wherein one end of the liquid draining pipe is communicated with the inner side of the dishes, one end of the liquid draining pipe is connected with a liquid draining cover in a threaded mode, one side of the fixed box is provided with a moving groove, the inner side of the fixed box is fixedly provided with a sliding rod, the outer side of the sliding rod is fixedly connected with a moving block in a sliding mode, the moving block penetrates through one end of the fixed box respectively and is fixedly connected with the inner side of a sliding groove respectively, one side of the moving block is fixedly provided with a holding rod inside the fixed box, the holding rod is fixedly connected with the inner side of the moving groove in a sliding mode, the outer side of the sliding rod is sleeved with a spring, one end of the spring is fixedly arranged on one side of the moving block respectively, and the other end of the spring is fixedly arranged on the inner side of the fixed box respectively.
In some embodiments, the inner sides of the sealing cover bodies are provided with internal threads, and the outer lower ends of the dish bodies are provided with external threads matched with the internal threads.
In some embodiments, transparent observation windows are arranged on the inner sides of the sealing cover bodies at the middle section positions.
In some embodiments, the inner sides of the sealing cover bodies are provided with liquid injection ports.
In some embodiments, a plurality of the moving blocks are respectively matched with a plurality of sliding grooves.
In some embodiments, a plurality of the grips are embodied as regular pentagons.
Compared with the prior art, the utility model, its apparent advantage is:
the method comprises the following steps: according to the utility model, the plurality of dish bodies are arranged, so that a plurality of cells can be cultured at the same time, the cell culture efficiency is improved, the holding rod is held for pulling, the moving block fixed by the holding rod is driven to synchronously move, and the moving block is further separated from the sliding groove, so that the circulation of cell culture waste liquid is facilitated, the cell culture waste liquid flows into the position below the fixed plate through the sliding groove, and then is discharged through the plurality of liquid discharge pipes;
and two,: according to the utility model, the liquid injection port is arranged, so that the liquid injection port can be closed through the sealing plug when the liquid injection port is not used, the sealing property of the dish body is ensured, the sealing plug can be opened when new cell culture liquid needs to be placed, and the new cell culture liquid is injected through the injector, so that the cell is prevented from being contacted with external air in the culture process, and the cell culture quality is prevented from being influenced;
the problem of current be inconvenient for discharging the waste liquid that the inside experiment of culture dish produced, unable to stack a plurality of culture dishes and place, occupation space is great is solved.
Drawings
The utility model is further explained below with reference to the drawings and examples:
FIG. 1 is a schematic view of a stacked dish according to an embodiment of the present utility model;
FIG. 2 is a schematic view of one of the dish structures according to an embodiment of the present utility model;
FIG. 3 is a schematic view of the internal structure of one of the dishes provided in one embodiment of the utility model;
FIG. 4 is a schematic view of the internal cross-sectional structure of one of the holding cassettes provided in one embodiment of the utility model;
FIG. 5 is a schematic diagram of the cross-sectional structure of one of the dishes according to the utility model, in one embodiment.
Reference numerals illustrate:
1. a dish body; 2. sealing cover; 3. a fixing plate; 4. a chute; 5. a fixed box; 6. a liquid discharge pipe; 7. a liquid discharge cover; 8. a moving groove; 9. a slide bar; 10. a moving block; 11. a grip; 12. a spring; 13. an internal thread; 14. an external thread; 15. a transparent viewing window; 16. and a liquid injection port.
Detailed Description
The following detailed description of the present utility model clearly and fully describes the technical solutions of the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The utility model provides a multilayer cell culture dish with a drainage groove through improvement, which comprises the following technical scheme:
as shown in fig. 1-3, a multi-layer cell culture dish with drainage grooves comprises a plurality of dish bodies 1, wherein the upper sides of the dish bodies 1 are respectively in threaded connection with a sealing cover 2, the sealing performance is better through the threaded connection, the inner sides of the dish bodies 1 are fixedly provided with fixing plates 3 for placing cells, the inner sides of the fixing plates 3 are provided with sliding grooves 4 at one end position, and the cell culture dish further comprises; the liquid discharging mechanisms are respectively arranged above the plurality of fixing plates 3; the flowing back mechanism is including being fixed in the fixed box 5 in the outside of a plurality of disk body 1, set up in the flowing back pipe 6 of a plurality of disk body 1 sides, the one end of a plurality of flowing back pipes 6 all communicates with the inboard of a plurality of disk body 1, and the equal spiro union of a plurality of flowing back pipe 6 outside one end has flowing back lid 7, open flowing back lid 7 and can go out the ejection of compact to the waste liquid, easy operation, the movable tank 8 has all been seted up to one side of a plurality of fixed boxes 5, and the inboard of a plurality of fixed boxes 5 all is fixed with slide bar 9, the equal sliding connection in the outside of a plurality of slide bar 9 has movable block 10, a plurality of movable block 10 run through sliding connection respectively in a plurality of fixed box 5 one end, and a plurality of movable block 10 respectively sliding connection are inboard in a plurality of spout 4, one side of a plurality of movable block 10 is located a plurality of fixed box 5 inside all is fixed with holding rod 11, a plurality of holding rod 11 respectively sliding connection are inboard in a plurality of movable tank 8, through holding rod 11 and pulling, it moves movable block 10, spill chute 4, carry out cell culture waste liquid to discharge to fixed plate 3 below, and the outside a plurality of slide bar 9 is equipped with a plurality of springs 12, a plurality of springs 12 are all set up in a plurality of springs 12, a plurality of springs are fixed to a plurality of spring pieces are moved to the inside one side, a plurality of spring pieces are set up respectively, a plurality of spring pieces are moved to the other end is reset spring 10, and a plurality of spring pieces are moved, and one end is fixed to be moved.
As shown in fig. 1, in an embodiment, the inner sides of the sealing cover bodies 2 are provided with internal threads 13, the outer lower ends of the dish bodies 1 are provided with external threads 14 matched with the internal threads 13, so that the dish bodies 1 are conveniently stacked in a screwed manner, the placing space is saved, and the dish bodies are more stable through screwing.
As shown in fig. 1-2, in an embodiment, in order to facilitate the monitoring of the cell culture by the staff during the cell culture, transparent observation windows 15 are disposed on the inner sides of the sealing covers 2 at the middle section, and the discharge of the waste liquid can be monitored during the replacement of the cell culture liquid through the transparent observation windows 15.
As shown in fig. 1-2, in an embodiment, the inner sides of the sealing cover bodies 2 are provided with the liquid injection ports 16, the sealing plugs are tightly plugged, the liquid injection ports 16 can be closed, and meanwhile, new cell culture liquid can be injected by taking down the sealing plugs through the cooperation of the injector and the liquid injection ports 16, so that the contact of cells with external air is avoided, and the culture quality of the cells is ensured.
As shown in fig. 5, in an embodiment, a plurality of moving blocks 10 are respectively matched with a plurality of sliding grooves 4; the closing effect of the chute 4 is ensured.
In one embodiment, as shown in fig. 4, in order to make it more convenient for a worker to hold the grip lever 11 and pull, the plurality of grip levers 11 are in particular regular pentagons, so that sliding during holding is effectively avoided.
The specific working method is as follows: during the use, place the cell that needs to cultivate in fixed plate 3 upside, add appropriate amount of cell culture solution, seal it through sealed lid 2, seal plug in annotating liquid mouth 16 inboard, when needs cultivate different cells, can place different cells in a plurality of fixed plate 3 upside respectively, thereby can cultivate multiple different cells simultaneously, experimental efficiency has been improved, the outside lower extreme of a plurality of dishes body 1 all is provided with external screw thread 14, the inboard of a plurality of sealed lid body 2 all is provided with the internal screw thread 13 with a plurality of external screw thread 14 looks adaptation, thereby through the spiro union, can pile up a plurality of dishes body 1 and place, practice thrift the space of placing, in cell culture process, often need change cell culture solution, when needs to change, the staff will need carry out the liquid discharge lid 7 of flowing down of dish body 1 side, hold down the holding rod 11 that corresponds with it outwards pulls, holding rod 11 is fixed in movable block 10 one side, thereby it moves movable block 10, movable block 10 and set up in movable block 3 inboard and set up in movable block 4 separation piece, simultaneously, can take out through the movable window 12 through the cell culture solution through the sealing plug 12, simultaneously, can be reached through the syringe that the cell culture liquid can be realized through the syringe, the improvement of the practical condition is realized, the cell culture solution is realized through the syringe groove 12, can be realized through the improvement of the cell culture liquid outlet channel is realized through the following the cell culture liquid groove 12, the improvement, the cell culture liquid is replaced.
The technical means disclosed by the scheme of the utility model is not limited to the technical means disclosed by the technical means, and also comprises the technical scheme consisting of the technical characteristics and the equivalent substitution. The present utility model is not limited to the prior art.
Claims (6)
1. The utility model provides a multilayer area drainage groove cell culture dish, includes a plurality of components of a whole that can function independently (1), a plurality of the equal spiro union of upside of components of a whole that can function independently (1) has sealed lid (2), and the inboard of components of a whole that can function independently (1) is fixed with fixed plate (3), a plurality of spout (4) have been seted up in the inboard of fixed plate (3) is located one end position department, its characterized in that: also comprises;
the liquid draining mechanisms are respectively arranged at the positions above the plurality of fixed plates (3);
the liquid draining mechanism comprises a fixed box (5) fixed to the outer side of a plurality of dish bodies (1), a plurality of liquid draining pipes (6) arranged on one side of the dish bodies (1), wherein one ends of the liquid draining pipes (6) are communicated with the inner sides of the plurality of dish bodies (1), a liquid draining cover (7) is fixedly connected to one end of the outer portion of the plurality of liquid draining pipes (6) in a threaded mode, a plurality of holding rods (11) are fixedly arranged on one side of the fixed box (5) respectively, moving grooves (8) are formed in one side of the fixed box (5), sliding rods (9) are fixedly arranged on the inner sides of the plurality of fixed box (5), moving blocks (10) are fixedly connected to one ends of the plurality of fixed box (5) in a sliding mode respectively, the moving blocks (10) are fixedly connected to the inner sides of the plurality of sliding grooves (4) respectively in a sliding mode, one sides of the moving blocks (10) are fixedly arranged on the inner sides of the plurality of fixed box (5), and a plurality of springs (12) are fixedly arranged on one sides of the inner sides of the sliding rods (9) respectively, and one ends of the springs (12) are fixedly arranged on one sides of the plurality of the spring sleeves (12).
2. A multilayer cell culture dish with drainage grooves according to claim 1, wherein: the inner sides of the sealing cover bodies (2) are provided with internal threads (13), and the lower ends of the outer sides of the dish bodies (1) are provided with external threads (14) matched with the internal threads (13).
3. A multilayer cell culture dish with drainage grooves according to claim 1, wherein: transparent observation windows (15) are arranged on the inner sides of the sealing cover bodies (2) at the middle section positions.
4. A multilayer cell culture dish with drainage grooves according to claim 1, wherein: the inner sides of the sealing cover bodies (2) are provided with liquid injection ports (16).
5. A multilayer cell culture dish with drainage grooves according to claim 1, wherein: the plurality of moving blocks (10) are respectively matched with the plurality of sliding grooves (4).
6. The multilayer cell culture dish with drainage channel of claim 5, wherein: the plurality of grips (11) are embodied as regular pentagons.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322137532.7U CN220618930U (en) | 2023-08-10 | 2023-08-10 | Multilayer cell culture dish with drainage groove |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322137532.7U CN220618930U (en) | 2023-08-10 | 2023-08-10 | Multilayer cell culture dish with drainage groove |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220618930U true CN220618930U (en) | 2024-03-19 |
Family
ID=90219109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322137532.7U Active CN220618930U (en) | 2023-08-10 | 2023-08-10 | Multilayer cell culture dish with drainage groove |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220618930U (en) |
-
2023
- 2023-08-10 CN CN202322137532.7U patent/CN220618930U/en active Active
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