CN220068515U

CN220068515U - Equipment for auxiliary culture and inspection of liver cancer animal model

Info

Publication number: CN220068515U
Application number: CN202320970606.2U
Authority: CN
Inventors: 关向前
Original assignee: Anhui Provincial Hospital First Affiliated Hospital Of Ustc
Current assignee: Anhui Provincial Hospital First Affiliated Hospital Of Ustc
Priority date: 2023-04-25
Filing date: 2023-04-25
Publication date: 2023-11-24
Anticipated expiration: 2033-04-25

Abstract

The utility model provides a liver cancer animal model auxiliary culture inspection device, which comprises a box body, wherein an experimental mouse culture inspection chamber is arranged in the box body, the culture inspection chamber comprises a fixed cavity, the fixed cavity comprises a fixed component, the fixed component comprises a pair of side plates which are arranged in parallel and opposite, a reversible clamping plate which is vertically connected between the pair of side plates and is used for fixing an experimental mouse, an inclined plate frame which is obliquely arranged in the horizontal direction and is used for supporting and fixing the side plates, a supporting plate which is longitudinally arranged below the clamping plate and a head kneading rod and a trunk kneading rod which are used for kneading and fixing the skin of the experimental mouse are arranged on the supporting plate; the weighing cavity is connected with the fixed assembly through the moving assembly; and an inspection chamber including a diagnostic device, the laboratory mouse being transferred to a position longitudinally below the diagnostic device by the moving assembly for inspection and diagnosis.

Description

Equipment for auxiliary culture and inspection of liver cancer animal model

Technical Field

The utility model relates to the technical field of research on animal models of human diseases, in particular to equipment for auxiliary culture and inspection of liver cancer animal models.

Background

Liver cancer is one of the most common tumors of human beings, and has hidden morbidity, short course of disease and extremely high morbidity and mortality. The preparation of a good liver cancer animal model is an important precondition for diagnosing and researching liver cancer by human beings, and the construction of the liver cancer animal model by gastric lavage or intraperitoneal injection induction of an experimental mouse is one of the common means for knowing and diagnosing liver cancer.

Chinese patent CN201810450157.2 discloses a stomach mount is irritated to medical science experiment mice, fixed plate one end is arranged in on the bottom plate, it has the buffer tank to open on the bottom plate, it has the sliding tray to open on the fixed plate other end, two arc cardboard one sides of fixed plate are arranged in respectively, arc cardboard one end is connected with the bottom plate, the fixed slot has been put to the arc cardboard inboard, the opposite side of two arc cardboard is arranged in respectively to two edge pterygoid lamina, edge pterygoid lamina one end is connected with the bottom plate, open in the edge pterygoid lamina outside has two draw-in grooves, and upper and lower distribution, main fixture block card is arranged in the draw-in groove of leaning on, auxiliary fixture block card is arranged in the draw-in groove of leaning on down, the both sides of main fixed band are connected through elastic strip and two main fixture blocks respectively, it has the movable tank to open on the fixed plate to assist the both sides of fixed band respectively, and be close to the movable tank, the connecting rod runs through the movable tank, the sliding block is arranged in one end of the connecting rod, and is located the fixed plate inboard, the sliding block is by arc block structure, and the medial surface is towards main fixed band, the regulating block is arranged on the connecting rod other end, one side of the connecting rod is arranged in the outside the sliding block, be the platy structure, and extend to the arc supporting plate is in the opposite side.

When the test mouse is subjected to gastric lavage, the gastric lavage device needs to be prevented from being inserted into the trachea of the test mouse and whether the gastric lavage device reaches the stomach of the test mouse or not by observing the fluctuation of the neck and the abdomen of the test mouse. Although the technical scheme has simple structure, the efficacy rate of the lavage of the test mouse can be effectively improved, the arc-shaped fixing device which can fix the neck and the abdomen of the test mouse can shield the observation of the test mouse by an experimenter, and the diagnosis of the lavage of the test mouse is not facilitated.

Chinese patent CN202120065319.8 discloses a convenient fixed experimental mouse experiment gastric lavage mechanism, including fixed plate, positioner, strutting arrangement and standing groove, positioner includes first clamp, first cavity, spring, restriction pole, restriction piece, first rectangular hole, second cavity, limiting plate, bolt, second rectangular hole, second clamp, third cavity, screw rod, knob, slider and third rectangular hole, strutting arrangement includes bracing piece, first connecting rod, second connecting rod, draw-in groove and buckle. Through setting up positioner, can restrict fixedly to the laboratory mouse of different body types or kind, easy operation. Through setting up strutting arrangement, the laboratory glassware can put the desktop after fixed with the laboratory glassware, perhaps after mentioning the laboratory glassware through reverse strutting arrangement, again carry out the operation of irritating the stomach, can make the operation of irritating the stomach more nimble when guaranteeing to test the mouse can not hurt the laboratory glassware like this. According to the technical scheme, although the experimental mouse is lifted by the reverse rotation supporting device and then is subjected to gastric lavage, the injury of the experimental mouse to experimental staff is avoided, the clamp above the placing groove of the fixing device still shields the experimental mouse partially, the observation of the experimental mouse is not facilitated, and meanwhile, the environment for culturing and checking the experimental mouse is not provided.

Therefore, the design and research of the device not only can realize the shielding-free fixed test mouse, but also has the important importance of the laboratory staff on the environment for the culture and anatomical inspection of the test mouse.

Disclosure of Invention

The utility model aims to solve the technical problem of providing equipment for realizing the non-shielding type fixed test mice and meeting the environment of laboratory staff on laboratory mice culture and inspection.

In order to solve the technical problems, the utility model provides a liver cancer animal model auxiliary culture inspection device, which comprises a box body, wherein the box body comprises a laboratory mouse culture inspection chamber, and the culture inspection chamber comprises:

the fixing cavity comprises a fixing assembly, wherein the fixing assembly comprises a pair of side plates which are arranged in parallel and opposite, a reversible clamping plate which is vertically connected between the pair of side plates and is used for fixing a laboratory mouse, an inclined plate frame which is obliquely arranged in the horizontal direction and is used for supporting and fixing the side plates, a supporting plate which is longitudinally arranged below the clamping plate and a head kneading rod and a trunk kneading rod which are arranged on the supporting plate and are used for kneading and fixing the skin of the laboratory mouse;

the weighing cavity is connected with the fixed assembly through the moving assembly; and

and the test cavity comprises a diagnosis device, and the test mouse is transferred to the position longitudinally below the diagnosis device through the moving assembly for test diagnosis.

Further, a support bracket for supporting arms of experimental staff is arranged on one side of the top edge of the fixed cavity.

Further, a driving shaft is connected between the clamping plate and the side plate, and a rotating disc is sleeved on the driving shaft.

Further, an adjusting groove is formed in the clamping plate.

Further, a pair of opposite inner sides of the side plates are provided with opposite gear transmission mechanisms which are in the shape of rounded triangles, the gear transmission mechanisms are vertically connected with the transmission rods and the supporting plates, and telescopic pieces used for enabling the longitudinal displacement of the supporting plates to be in contact with the backs of the experimental rats are vertically arranged in the middle of the transverse directions of the supporting plates.

Further, a pair of longitudinal middle positions of the side plates are provided with a moving groove and a positioning groove which penetrate through the side plates and are parallel to each other, the transmission rod penetrates through the moving groove to be connected with a transmission handle, the transmission handle is vertically and rotatably connected with a positioning rod, and the positioning rod is fixedly inserted into the positioning groove.

Further, the gear transmission mechanism comprises a driving piece, a driven piece and a linkage piece, wherein the driving piece and the driven piece are meshed with each other, and the linkage piece is connected with the driving piece through a transmission belt.

Further, the moving assembly comprises a clamping cover and a lifting platform vertically fixed with one side of the clamping cover, and the lifting platform is vertically arranged on the horizontal moving guide rail to move horizontally.

Further, the clamping cover is fixedly inserted into the slot, and the slot is arranged on one side of the fixing assembly.

Preferably, the inspection cavity further comprises sterilizing pieces, wherein the sterilizing pieces are uniformly distributed at the top edge inside the clamping cover at intervals and used for sterilizing the fixing assembly.

Preferably, the box body further comprises a first contrast chamber and a second contrast chamber.

Preferably, the case body includes:

a cleaning chamber provided at one side outside the cabinet for sterilizing external devices entering the incubation inspection chamber;

the waste liquid storage chamber comprises a liquid collecting cavity arranged at one side outside the box body, and the liquid collecting cavity is connected with the cleaning chamber and the fixing cavity through a conduit in a conducting way.

Further, the cleaning chamber is of a drawing type structure.

Further, an operation opening is formed in one side of the box body, and a hand wearing protection piece is arranged at the operation opening.

Preferably, the side wall of the box body is provided with a ventilation opening.

Further, an observation window is arranged at the top of the box body; the side wall of the box body is provided with a box body door.

A method for liver cancer animal model assisted culture inspection, comprising liver cancer animal model assisted culture inspection equipment, the method comprising the following steps:

s1, observation and culture: placing a laboratory mouse into the culture laboratory for observation culture before constructing a liver cancer model;

s2, back fixing: when the liver cancer model is constructed by performing stomach lavage and intraperitoneal injection on the experimental mouse, an experimenter holds the experimental mouse in the clamping plate by pinching the back of the experimental mouse by hand;

s3, turning and observing: starting a driving source to drive the clamping plate to turn over for half a circle, so that the face of the experimental mouse faces upwards;

s4, fixing the layers: the supporting plate is lifted by the telescopic piece, so that the head kneading rod and the trunk kneading rod at the inner side of the supporting plate are contacted with the back of the experimental mouse and then rotate in opposite directions, and further the skin and the muscle at the back of the experimental mouse are limited and fixed by the rolling of the head kneading rod and the trunk kneading rod which rotate in opposite directions;

s5, auxiliary gastric lavage: after the experimental mouse is fixed, the head of the fixed experimental mouse is inclined upwards through the fixing component, and then the experimental mouse is matched with the support bracket for supporting the arms of the experimental staff, so that the gastric lavage operation of the experimental mouse with the inclined head upwards is completed on the premise that the arms of the experimental staff are supported;

s6, checking and diagnosing: and the fixing component is clamped through the clamping cover, so that the diagnosis equipment above the clamping cover in the longitudinal direction performs inspection and diagnosis on the laboratory mice in the fixing component.

Compared with the prior art, the utility model has the following beneficial effects:

1. according to the utility model, the telescopic piece is controlled to ascend through the control operation panel on the side wall of the box body, the supporting plate, the head kneading rod, the trunk kneading rod and the back of the experimental mouse are contacted, the right hand is used for controlling the transmission handle to rotate anticlockwise, the linkage piece drives the two groups of trunk kneading rods and the head kneading rod on the inner side of the supporting plate to rotate oppositely, the back skin of the experimental mouse is rolled and kneaded to be fixed, the head kneading rod and the trunk kneading rod are enabled to be attached to the back of the head and neck and trunk parts of the experimental mouse through the design that the diameter of the head kneading rod is larger than that of the trunk kneading rod, the head kneading rod and the trunk kneading rod are enabled to roll and knead the back of the head of the experimental mouse, extrusion damage of the stomach irrigator to the internal organ of the experimental mouse caused by discomfort struggling generated by the stomach irrigation of the experimental mouse is reduced, and meanwhile, the head of the experimental mouse is relatively lifted through the design that the diameter of the head kneading rod is larger than that the trunk kneading rod is also, so that the difficulty of the stomach irrigation is reduced. Further, the experimenter is through placing the right hand on supporting the support and the fixed subassembly slope sets up, more is favorable to the operation of laboratory mouse lavage.

2. According to the utility model, the experimental mouse is fixed, overturned and dissected through the fixing component, the lifting piece is driven to be close to the weighing cavity through the moving component after dissection is completed, the clamping cover at the side part of the lifting piece corresponds to the top of the slot, the lifting piece is lowered, the clamping cover is clamped into the slot, the transparent plate inside the clamping cover corresponds to the top of the fixing component, the diagnostic piece arranged at the top of the clamping cover is used for carrying out inspection and diagnosis on the liver of the experimental mouse through the transparent plate, and the culture and dissection inspection and diagnosis of the experimental mouse are carried out in the same environment, so that the interference of the experiment mouse transfer environment on the inspection and diagnosis result is reduced, and the sterilizing piece arranged inside the clamping cover can carry out clamping and sterilization on the fixing component of the diagnosed taken-away experimental mouse, thereby being convenient for subsequent reuse.

Drawings

FIG. 1 is a front view of the apparatus of the present utility model;

FIG. 2 is a side view of the apparatus of the present utility model;

FIG. 3 is a top view of the apparatus of the present utility model;

FIG. 4 is a schematic view of the structure of the stationary chamber of the apparatus of the present utility model;

FIG. 5 is a schematic view of the structure of the fixing assembly of the fixing chamber of the apparatus of the present utility model;

FIG. 6 is an enlarged view of the structure of the fixing member of the fixing chamber of the apparatus of the present utility model;

FIG. 7 is an enlarged view of the structure of the clamping plate of the fixing assembly of the fixing chamber of the apparatus of the present utility model;

FIG. 8 is a schematic view showing the structure of a head kneading bar and a trunk kneading bar of a fixing member of a fixing chamber of the apparatus of the present utility model;

FIG. 9 is a schematic view of the gear mechanism of the stationary assembly of the stationary chamber of the apparatus of the present utility model;

FIG. 10 is a schematic view of the structure of the inspection chamber of the apparatus of the present utility model;

FIG. 11 is a schematic view of the structure of a clean room of the apparatus of the present utility model;

FIG. 12 is an enlarged view of the structure of the clean room of the apparatus of the present utility model;

fig. 13 is a flow chart of a method of operation of the apparatus of the present utility model.

In the figure: 1. a case; 1a, an observation table; 1b, a box door; 1c, an operation panel; 1d, a ventilation port; 1e, an operation port; 1e1, hand wear protector; 11. culturing a test room; 11a, a fixed cavity; 11a1, side plates; 11a11, a moving groove; 11a12, a positioning groove; 11a2, a clamping plate 11a21, and an adjusting groove; 11a22, connecting plates; 11a23, put into the groove; 11a24, clamping grooves; 11a25, external groove; 11a26, closing blocks; 11a3, a support plate; 11a31, a head skin kneading bar; 11a32, torso skin pinching bars; 11a33, a telescopic member; 11a4, a swash plate frame; 11a5, a support bracket; 11a6, a drive shaft; 11a61, a rotating disc; 11a7, a transmission rod; 11a71, a transmission handle; 11a72, a positioning rod; 11a8, a gear transmission mechanism; 11a81, an active member; 11a82, a follower; 11a83, a linkage; 11b, weighing cavity; 11b1, a snap-in cover; 11b2, lifting table; 11c, inspection chamber; 11c1, diagnostic equipment; 11c2, a killing member; 12. a first contrast chamber; 13. a second contrast chamber; 2. a cleaning chamber; 21. a disinfection chamber; 211. a sealing plate; 22. a sterilizing member; 23. a rolling rod; 24. a restraint seat; 241. a semi-open round sleeve; 25. a traction rail; 3. a waste liquid storage chamber; 31. a liquid collecting cavity.

Detailed Description

In order to make the technical solutions and technical effects of the present utility model more clear, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the drawings in the embodiments, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments.

The utility model provides a device for auxiliary culture inspection of liver cancer animal models, which is shown in a front view of the device in FIG. 1, and comprises a box body 1, wherein a culture inspection chamber 11 is arranged in the box body 1; the outside of the box body 1 is communicated with the box body 1 and provided with a cleaning chamber 2 and a waste liquid storage chamber 3. The waste liquid storage chamber 3 is connected vertically below the cleaning chamber 2 by a conduit. An operation panel 1c is arranged on the side wall of one side of the box body 1, two operation ports 1e are arranged below the operation panel 1c, and hand wearing protection pieces 1e1 are arranged at the operation ports 1 e. The top of the box body 1 is provided with an observation table 1a, and the right front side of the box body 1 is provided with a box body door 1b.

The clean room 2 is used to kill items (such as a gastric lavage device or a syringe) that enter the housing 1, thereby reducing the effect of external equipment on the internal sterile environment after entering the incubation test room 11.

The waste liquid storage chamber 3 is used for collecting waste liquid of the clean room 2 and the incubation inspection chamber 11 in the case 1.

As shown in fig. 2, which is a side view of the apparatus of the present utility model, a ventilation opening 1d is provided above the side wall of the casing 1. The cleaning chamber 2 is arranged perpendicular to the box body 1 and is of a drawing type structure.

As shown in FIG. 3, in a top view of the apparatus of the present utility model, a culture inspection chamber 11 is provided inside the case 1, and a first contrast chamber 12 and a second contrast chamber 13 are provided adjacent to the culture inspection chamber 11.

The culture checking chamber 11 includes a fixed chamber 11a, a weighing chamber 11b adjacent to the fixed chamber 11a, and a checking chamber 11c. A support bracket 11a5 for supporting the arms of the experimenters is arranged on one side of the top edge of the fixing cavity 11 a.

As shown in fig. 4 and 5, the fixing chamber 11a of the apparatus of the present utility model is schematically structured, the fixing chamber 11a includes a fixing assembly including: a pair of side plates 11a1 arranged in parallel and opposite, a reversible clamping plate 11a2 vertically connected between the pair of side plates 11a1 for fixing a laboratory mouse, a sloping plate frame 11a4 arranged obliquely in the horizontal direction for supporting and fixing the side plates 11a1, a supporting plate 11a3 arranged in parallel and longitudinally below the clamping plate 11a2, and a head kneading rod 11a31 and a trunk kneading rod 11a32 for kneading and fixing the skin of the laboratory mouse are mounted on the supporting plate 11a 3.

As shown in fig. 6 and 7, a schematic structural diagram of a clamping plate of a fixing assembly of a fixing cavity 11a of the device of the present utility model is shown, a driving shaft 11a6 is connected between side plates 11a1 of the clamping plate 11a2, a rotating disc 11a61 is sleeved on the driving shaft 11a6, a driving source is mounted on the upper side wall of one side plate, and the driving source drives the clamping plate 11a2 to turn. Preferably, the driving source is a direct current gear motor controlled by a PLC encoder, and the direct current gear motor is externally connected with an external power supply (not shown in the drawing). In operation, the drive source is activated to turn the holding plate 11a2 over half a turn, with the face of the laboratory mouse facing upward. The space between the clamping plates 11a2 can not shade the abdomen and the neck of the laboratory mouse, so that the laboratory staff can observe the real-time reaction condition of the laboratory mouse during stomach irrigation and intraperitoneal injection in time, the abdomen and the neck of the laboratory mouse can not be restrained, and the unsmooth gastrointestinal stress of the laboratory mouse caused by restraining and fixing extrusion is reduced.

The left side and the right side of the clamping plate 11a2 in the fixing component are symmetrically provided with adjusting grooves 11a21, and the adjusting grooves 11a21 adjust the spacing of the clamping plate 11a2 according to different sizes of laboratory rats. The middle part of the adjusting groove 11a21 is vertically provided with a placing groove 11a23 on the connecting plate 11a22, the connecting plate 11a22 is vertically provided with a clamping groove 11a24, and a closing block 11a26 is arranged in an external connecting groove 11a25 parallel to the placing groove 11a23, and the closing block 11a26 is preferably made of elastic rubber materials. In operation, the experimenter holds the back of the experimental mouse by hand, and fixes the experimental mouse in the holding plate 11a 2.

As shown in fig. 9, the gear transmission mechanism 11a8 of the fixing assembly of the fixing chamber of the apparatus of the present utility model is schematically structured, and the opposite inner sides of a pair of side plates 11a1 in the fixing assembly are mounted with the opposite gear transmission mechanism 11a8 having a rounded triangle shape. The gear transmission mechanism 11a8 includes a driving member 11a81 and a driven member 11a82 which are engaged with each other, and a link member 11a83 which is connected to the driving member 11a81 by a belt. A transmission rod 11a7 and a supporting plate 11a3 are vertically connected between the two gear transmission mechanisms 11a8, a telescopic piece 11a33 is vertically arranged in the middle of the transverse direction of the supporting plate 11a3, and the telescopic piece 11a33 is used for adjusting the longitudinal displacement of the supporting plate 11a3 to enable the supporting plate 11a3 to be in contact with the back of the laboratory mouse. Preferably, the telescopic members 11a33 are electric telescopic rods controlled by a PLC.

The longitudinal middle positions of the pair of side plates 11a1 are respectively provided with a moving groove 11a11 and a positioning groove 11a12 penetrating through the surfaces of the side plates, a transmission rod 11a7 penetrates through the moving groove 11a11 to be connected with a transmission handle 11a71, a positioning rod 11a72 is vertically and rotatably connected with the transmission handle 11a71, and the positioning rod 11a72 is fixedly inserted into the positioning groove 11a 12.

The head kneading rod 11a31 and the trunk kneading rod 11a32 of the fixing member of the fixing chamber 11a of the apparatus of the present utility model as shown in FIG. 8 are schematically constituted, and the head kneading rod 11a31 and the trunk kneading rod 11a32 can be made to fit more closely to the back of the head and neck and trunk parts of the laboratory mouse by designing the diameter of the head kneading rod 11a31 to be larger than the diameter of the trunk kneading rod 11a32, so that the head kneading rod 11a31 and the trunk kneading rod 11a32 can perform rolling-kneading restriction on the head and back of the laboratory mouse. And then reduce the uncomfortable struggling that the laboratory mouse irritates the stomach and shake the extrusion damage of the stomach-filling device that leads to laboratory mouse internal organ, the diameter of head kneading rod 11a31 is greater than the diameter design of truck kneading rod 11a32 and also can make the head of laboratory mouse relatively raised simultaneously, and then has reduced the degree of difficulty of irritating the stomach.

As shown in fig. 10, a schematic structural diagram of a checking cavity 11c of the device of the present utility model is shown, a moving assembly is installed on the bottom surface of the checking cavity 11c, the top of the moving assembly is movably connected with a side table through a lifting table 11b2, and the lifting table 11b2 is vertically installed on a horizontal moving guide rail to move horizontally. The side part of the side table is fixedly connected with a clamping cover 11b1, and disinfecting and killing pieces 11c2 are uniformly distributed at intervals on the edge of the top end inside the clamping cover 11b1 and used for disinfecting and sterilizing the fixing assembly. A transparent plate is arranged in the middle of the clamping cover 11b 1; the clamping cover 11b1 is movably connected with the migration block through an adjusting seat, a stand column is arranged at the top of the migration block, and a diagnostic device 11c1 is arranged on the stand column.

The culture and the anatomical inspection and diagnosis of the laboratory mice are carried out in the same environment, so that the interference of the transfer environment of the laboratory mice on inspection and diagnosis results is reduced, and the disinfecting piece 11c2 arranged on the clamping cover 11b1 can disinfect the fixed assembly of the laboratory mice after the diagnosis is finished, so that the laboratory mice are convenient to reuse subsequently.

As shown in fig. 11 and 12, the cleaning chamber 2 of the device of the present utility model is schematically shown, the cleaning chamber 2 is communicated with the culture inspection chamber 11 in the case 1, the cleaning chamber 2 comprises a disinfection chamber 21 and a sealing plate 211, the front and rear side walls of the disinfection chamber 21 are symmetrically provided with traction rails 25, the inner side of the disinfection chamber 21 is fixedly connected with a constraint seat 24, the top of the constraint seat 24 is provided with a semi-open circular sleeve 241, and the semi-open circular sleeve 241 is preferably made of elastic plastic, so that the gastric lavage device or a marker pen is conveniently extruded and fixed in the semi-open circular sleeve 241.

A schematic flow chart of an operation method of the apparatus of the present utility model shown in fig. 13, the operation steps include:

first, observation culture: placing the laboratory mice into a culture inspection room 11 for observation culture before constructing a liver cancer model;

secondly, back fixing: when the liver cancer model is needed to be constructed by stomach irrigation and intraperitoneal injection, an experimenter wears the hand wearing protection piece 1e1 and then holds the back of the experimenter, so that the face of the experimenter is downwards placed in the oppositely arranged clamping plates 11a2, and the limbs of the experimenter penetrate through the placing groove 11a23 and are placed in the adjusting groove 11a 21;

thirdly, turning and observing: starting a driving source to drive the clamping plate 11a2 to turn over for half a turn, so that the back of the experimental mouse faces downwards and the face faces upwards, and fixing the experimental mouse by an experimenter without turning over the wrist in the process;

fourth, layer fixing: the supporting plate 11a3 is lifted by the telescopic piece 11a33, the experimenter controls the transmission handle 11a71 to rotate anticlockwise, so that the head kneading rod 11a31 and the trunk kneading rod 11a32 on the inner side of the supporting plate 11a3 are contacted with the back of the experimental mouse and then rotate oppositely, and further the skin and the muscle on the back of the experimental mouse are limited and fixed by the rolling of the head kneading rod 11a31 and the trunk kneading rod 11a32 which rotate oppositely;

fifth step, auxiliary gastric lavage: after the experimental mouse is fixed, the head of the fixed experimental mouse is inclined upwards through the fixing component, and then the experimental mouse is matched with the support bracket 11a5 for supporting the arms of the experimenters, so that the stomach filling operation of the experimental mouse with the inclined head upwards is completed on the premise that the arms of the experimenters are supported;

sixth step, checking and diagnosing: the mice after the completion of the stomach irrigation or injection were placed in the first contrast chamber 12 or the second contrast chamber 13 for contrast observation. When the experimental mouse model needs to be dissected and diagnosed to check the liver condition of the experimental mouse, the experimental mouse can be fixed and overturned through the fixing component, the lifting table 11b2 is driven by the moving component to be close to the weighing cavity 11b after the dissection is completed, the clamping cover 11b1 on the side part of the lifting table 11b2 corresponds to the top of the slot, the lifting table 11b2 is lowered, the clamping cover 11b1 is clamped into the slot, the transparent plate inside the clamping cover 11b1 corresponds to the top of the fixing component, and the diagnostic equipment 11c1 arranged at the top of the clamping cover 11b1 is used for checking and diagnosing the liver of the experimental mouse through the transparent plate.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a liver cancer animal model assists equipment of cultivateing inspection, includes box (1), including laboratory mouse cultivate inspection room (11) in box (1), its characterized in that, cultivate inspection room (11) include:

the fixing device comprises a fixing cavity (11 a), wherein the fixing cavity (11 a) comprises a fixing assembly, the fixing assembly comprises a pair of side plates (11 a 1) which are arranged in parallel and opposite, a reversible clamping plate (11 a 2) which is vertically connected between the pair of side plates (11 a 1) and is used for fixing a laboratory mouse, an inclined plate frame (11 a 4) which is obliquely arranged in the horizontal direction and is used for supporting and fixing the side plates (11 a 1), a supporting plate (11 a 3) which is arranged below the clamping plate (11 a 2) in parallel in the longitudinal direction, and a head kneading rod (11 a 31) and a trunk kneading rod (11 a 32) which are used for kneading and fixing the skin of the laboratory mouse are arranged on the supporting plate (11 a 3);

a weighing cavity (11 b), the weighing cavity (11 b) being connected to the fixed assembly by a moving assembly; and

and a test chamber (11 c), wherein the test chamber (11 c) comprises a diagnosis device (11 c 1), and the test mouse is transferred to the position vertically below the diagnosis device for test diagnosis through the moving assembly.

2. The apparatus for auxiliary culture inspection of liver cancer animal model according to claim 1, wherein a support bracket (11 a 5) for supporting an arm of an experimenter is provided at one side of the top edge of the fixed cavity (11 a).

3. The liver cancer animal model assisted culture inspection device according to claim 1, wherein a driving shaft (11 a 6) is connected between the clamping plate (11 a 2) and the side plate (11 a 1), and a rotating disc (11 a 61) is sleeved on the driving shaft (11 a 6).

4. The apparatus for auxiliary culture inspection of liver cancer animal model according to claim 1, characterized in that the clamping plate (11 a 2) is provided with an adjusting groove (11 a 21).

5. The liver cancer animal model assisted culture inspection device according to claim 1, wherein a pair of side plates (11 a 1) are provided with opposite gear transmission mechanisms (11 a 8) with rounded triangles on opposite inner sides, a transmission rod (11 a 7) and a support plate (11 a 3) are vertically connected with the gear transmission mechanisms (11 a 8), and a telescopic piece (11 a 33) for longitudinally displacing the support plate (11 a 3) to be in contact with the back of an experimental rat is vertically arranged in the middle of the support plate (11 a 3) in the transverse direction.

6. The apparatus for auxiliary culture inspection of liver cancer animal models according to claim 5, characterized in that a moving groove (11 a 11) and a positioning groove (11 a 12) which are parallel to each other penetrating through the side plate (11 a 1) are provided at a longitudinal middle position of the pair of side plates (11 a 1), the transmission rod (11 a 7) penetrates through the moving groove (11 a 11) to be connected with a transmission handle (11 a 71), a positioning rod (11 a 72) is vertically and rotatably connected with the transmission handle (11 a 71), and the positioning rod (11 a 72) is inserted and fixed in the positioning groove (11 a 12).

7. The apparatus for auxiliary culture inspection of liver cancer animal model according to claim 5, wherein the gear transmission mechanism (11 a 8) comprises a driving member (11 a 81) and a driven member (11 a 82) engaged with each other and a linkage member (11 a 83) connected with the driving member (11 a 81) through a transmission belt.

8. The apparatus for auxiliary culture inspection of liver cancer animal model according to claim 1, wherein the moving assembly comprises a clamping cover (11 b 1) and a lifting table (11 b 2) vertically fixed to one side of the clamping cover (11 b 1), and the lifting table (11 b 2) is vertically installed to be horizontally displaced on a horizontally moving guide rail.

9. The apparatus for auxiliary culture inspection of liver cancer animal models according to claim 8, wherein the snap-in cover (11 b 1) and the slot (11 a 9) are inserted and fixed, and the slot (11 a 9) is disposed at one side of the fixing assembly.

10. The liver cancer animal model assisted culture inspection device according to claim 8, wherein the inspection cavity (11 c) further comprises sterilizing members (11 c 2), and the sterilizing members (11 c 2) are uniformly distributed at intervals on the top edge inside the clamping cover (11 b 1) and used for sterilizing the fixing assembly.

11. The apparatus for auxiliary culture inspection of liver cancer animal model according to claim 1, characterized in that the inside of the box (1) further comprises a first contrast chamber (12) and a second contrast chamber (13).

12. The apparatus for auxiliary culture inspection of liver cancer animal model according to claim 1, wherein the outside of the case (1) comprises:

a cleaning chamber (2), wherein the cleaning chamber (2) is arranged at one side outside the box body (1) and is used for disinfecting external equipment entering the culture checking chamber (11);

the waste liquid storage chamber (3), this waste liquid storage chamber (3) is including setting up gather liquid chamber (31) of the outer one side of box (1), gather liquid chamber (31) with clean room (2) with fixed chamber (11 a) are through pipe conduction connection.

13. The apparatus for auxiliary culture inspection of liver cancer animal model according to claim 12, characterized in that the clean room (2) is of a pull-out type structure.

14. The liver cancer animal model assisted culture inspection device according to claim 1, wherein an operation port (1 e) is provided on one side of the case (1), and a hand wearing protector (1 e 1) is provided at the operation port (1 e).

15. The liver cancer animal model assisted culture inspection device according to claim 1, wherein the side wall of the box body (1) is provided with a ventilation port (1 d).

16. The liver cancer animal model assisted culture inspection device according to claim 1, wherein an observation window (1 a) is arranged at the top of the box body (1); the side wall of the box body is provided with a box body door (1 b).