CN220056824U - Anti-pollution bacterial culture device - Google Patents
Anti-pollution bacterial culture device Download PDFInfo
- Publication number
- CN220056824U CN220056824U CN202321286698.9U CN202321286698U CN220056824U CN 220056824 U CN220056824 U CN 220056824U CN 202321286698 U CN202321286698 U CN 202321286698U CN 220056824 U CN220056824 U CN 220056824U
- Authority
- CN
- China
- Prior art keywords
- pipe
- barrel
- sample
- lamp tube
- sample adding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 3
- 238000007599 discharging Methods 0.000 abstract description 13
- 238000005070 sampling Methods 0.000 abstract description 8
- 241000894006 Bacteria Species 0.000 description 13
- 241000233866 Fungi Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 201000004624 Dermatitis Diseases 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229940124599 anti-inflammatory drug Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an anti-pollution bacterial culture device which comprises a culture tank, a sample adding assembly, a bacterial filtering and air discharging assembly and an aseptic liquid discharging mechanism, wherein a stirring motor and a vortex stirring paddle are arranged at the bottom of the culture tank, a liquid discharging pipe is arranged at one side of the bottom of the culture tank, a liquid feeding pipe is arranged at one side of the middle position of the upper half part of the culture tank, a heating plate is arranged on the periphery of the outer wall of the lower half part of the culture tank, the sample adding assembly comprises a long tube, a tube cover, an extending rod and a sample adding barrel, the bacterial filtering and air discharging assembly comprises an air discharging pipe, a bacterial filtering pipe and an air discharging valve pipe, manual valves are arranged on the liquid discharging pipe, the liquid feeding pipe and the air discharging pipe, and the aseptic liquid discharging mechanism comprises a temporary storage barrel, a liquid inlet pipe, a liquid discharging pipe and an exhaust pipe which are arranged on the barrel. The utility model has the advantages that the skin sample is completely sampled and placed into the sample adding barrel, the contact surface with the external air is small, the contact time period is short, the external pollution is greatly reduced, when the culture solution is sampled, the bacterial solution is quantitatively discharged into the temporary storage barrel, and the pollution problem of the culture tank during sampling is greatly reduced.
Description
Technical Field
The utility model relates to the technical field of bacterial culture on skin, in particular to an anti-pollution bacterial culture device.
Background
Bacterial culture is a technique for growing and breeding bacteria by an artificial method, and bacteria are extremely widely distributed in nature in a large number and variety, and can benefit mankind and also be a cause of pathogenicity, so that the bacteria need to be cultured. When the elimination of skin inflammation cannot be prevented by conventional anti-inflammatory drugs, it is sometimes necessary to sample the skin inflammation site, culture the bacteria, and examine the cultured bacteria to determine the kind or specific type of bacteria infected for targeted treatment. However, after the skin is sampled, the skin tissue is placed into a culture tank, so that other strains are easily infected, the number of mixed bacteria in the cultured strains is increased, the detection result is influenced, and skin tissue is directly placed into the culture tank, is easily precipitated to the bottom for adhesion, so that bacteria are not easy to flow into a culture solution, the culture effect is poor, the culture time is long, and the culture failure is easy to cause.
Disclosure of Invention
The present utility model aims to solve at least one of the technical problems in the related art to some extent. Therefore, an object of the utility model is to provide an anti-pollution bacterial culture device, which solves the problems that the skin tissue is collected and placed in a tank body to be cultured easily to be infected, the bacterial liquid after the culture is not easy to expand to be cultured, the bacterial liquid is easy to pollute, the sampling and the detection are difficult, and the like.
The utility model provides an anti-pollution bacterial culture device, which comprises a culture tank, a sample adding component, a bacterial filtering and air discharging component and a sterile liquid discharging mechanism, wherein the bottom of the culture tank is provided with a stirring motor and a vortex stirring paddle, a rotating shaft of the stirring motor is inserted into the bottom of the culture tank and sleeved with the vortex stirring paddle, one side of the bottom of the culture tank is provided with a liquid discharging pipe, one side of the middle position of the upper half part of the culture tank is provided with a liquid adding pipe, the outer wall of the lower half part of the culture tank is provided with a heating plate in a circle, the sample adding component comprises a long tube, a tube cover, an extending rod and a sample adding barrel, the top end of the extending rod is arranged on the inner top wall of the tube cover, the bottom end of the extending rod is fixed on the top of the sample adding barrel, the utility model provides a aseptic drain mechanism, including long bobbin, long bobbin top, filter fungus bleeder assembly, the application of sample bucket inserts from long bobbin top and inserts long bobbin bottom and expose, strain fungus bleeder assembly includes bleeder, strain fungus pipe and bleeder valve pipe, the bleeder is inserted on the cultivation jar top, strain fungus pipe upper and lower both ends respectively with bleeder valve pipe bottom and bleeder top spiral cup joint, it has the multi-disc strain membrane to strain fungus intraductal stopper, all be equipped with the manual valve on fluid-discharge tube, liquid feeding pipe and the bleeder valve pipe, aseptic drain mechanism includes feed liquor pipe, drain pipe and the blast pipe that sets up on barrel of keeping in, it is equipped with feed liquor pipe and drain pipe to keep in the barrel and is close to the bottom both sides, it is equipped with the blast pipe to keep in the barrel top, the feed liquor pipe inserts in the cultivation jar and slightly is higher than vortex stirring rake.
In some embodiments of the utility model, a sample adding port is arranged on one side of the upper half part of the sample adding barrel, a plurality of liquid inlet holes distributed in an array are arranged on the lower half part of the sample adding barrel, and when the sample adding barrel is positioned at the bottom end of the long barrel, the sample adding port is just positioned inside the bottom end of the long barrel.
In other embodiments of the present utility model, an illumination assembly is provided in the middle of the top of the culture tank, the illumination assembly includes a lamp tube, a lamp tube sleeve, and a long lamp tube, the long lamp tube is installed in the lamp tube sleeve, and the long lamp tube is inserted into the lamp tube to rotate the lamp tube sleeve on the lamp tube.
In other embodiments of the present utility model, a sterilization heating plate is provided on the outer wall of the temporary storage barrel.
In other embodiments of the present utility model, an observation window is provided on the outer wall of the temporary storage barrel.
In other embodiments of the utility model, the shaft of the agitator motor is provided with a mechanical seal on the bottom wall of the tank.
According to the utility model, the skin sample is completely sampled and placed into the sample adding barrel, the long barrel extends into the culture tank, the barrel cover is screwed, the sample adding is completed, the contact surface with the external air is small, the contact time period is shortened, the external pollution is greatly reduced, the skin tissue is not easy to adhere to the wall and is suspended in the culture solution, the culture effect is improved, when the culture solution is sampled, the bacterial solution is quantitatively discharged into the temporary storage barrel, the manual valve on the liquid inlet pipe is closed, and then the sample bacterial solution in the temporary storage barrel is taken out in batches by the sampling container, so that the pollution problem of the culture tank during sampling is greatly reduced.
Drawings
The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model. In the drawings:
FIG. 1 is a schematic diagram of an anti-pollution bacterial culture device according to the present utility model.
Fig. 2 is a schematic structural diagram of a sample loading barrel according to the present utility model.
In the figure: 1. a culture tank; 11. a heating sheet; 12. a liquid discharge pipe; 13. a liquid adding tube; 2. a stirring motor; 21. vortex stirring paddles; 3. a temporary storage barrel; 31. a liquid inlet pipe; 32. a liquid outlet pipe; 33. an exhaust pipe; 34. a sterilizing heating plate; 4. a long bobbin; 5. a sample adding barrel; 51. a sample adding port; 52. a liquid inlet hole; 6. a lamp tube sleeve; 61. a long lamp tube; 7. a gas discharge tube; 8. a bacterial filtering tube; 81. a bacterial filtering membrane; 9. and a release valve tube.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Examples of the embodiments are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements throughout or elements having like or similar functionality. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.
Referring to fig. 1-2, an anti-pollution bacteria culture apparatus, including cultivate jar 1, application of sample subassembly, strain air release subassembly and aseptic flowing back mechanism, cultivate jar 1 bottom and be equipped with agitator motor 2 and vortex stirring rake 21, the pivot of agitator motor 2 inserts in cultivate jar 1 bottom and in the pivot cover have vortex stirring rake 21, cultivate jar 1 bottom one side is equipped with fluid-discharge tube 12, cultivate jar 1 is equipped with liquid feeding pipe 13 in intermediate position one side in last half, cultivate jar 1 lower half outer wall a week and be equipped with heating plate 11, the application of sample subassembly includes long bobbin 4, bobbin lid 41, stretches into pole 42 and application of sample bucket 5, stretch into pole 42 top and fix on bobbin tube lid 41 inner top wall, the bottom is fixed at application of sample bucket 5 top, the application of sample bucket 5 inserts from long bobbin 4 top and inserts long bobbin 4 bottom and expose, strain air release subassembly includes air release pipe 7, strain pipe 8 and release valve 9, release pipe 7 inserts on cultivate jar 1 top, be equipped with liquid feeding pipe 8 and release valve 3 in the tip is equipped with in the tip of swirl pot 3, and the drain valve 3 is equipped with in the drain valve 31 in the drain pipe 3, and the drain valve 3 is equipped with the drain valve 3 and the drain valve 3.
The manual valves on the air release pipe 9 and the liquid adding pipe 13 are opened firstly, the culture liquid is injected through the liquid adding pipe 13, after the culture liquid is added, the manual valve on the liquid adding pipe 13 is closed, the heating plate 11 is used for heating the culture tank 1 (the bacteria filtering pipe and the air release pipe can be detached firstly), further sterilization is carried out, bacteria are killed, the bacteria filtering pipe 8 and the air release pipe 9 are installed in time, and after cooling is carried out, the manual valve on the air release pipe 9 is closed. When in sample application, the tube cover 41 is unscrewed, the sample application barrel 5 is taken out, the skin tissue sample is placed in the sample application barrel 5 and then extends into the long tube 4, and the tube cover 41 is screwed on, so that the sample application is completed. The culture temperature is regulated, and the culture is performed. After the culture is finished, when the culture solution is sampled, the bacterial solution is quantitatively discharged into the temporary storage barrel, a manual valve on the liquid inlet pipe 52 is closed, and then the sample bacterial solution in the temporary storage barrel 3 is taken out in batches by using a sampling container, so that the sampling is finished. Greatly reduces the pollution problem of the culture tank caused by sampling.
The upper half part of the sample adding barrel 5 is provided with a sample adding port 51, the lower half part of the sample adding barrel is provided with a plurality of liquid inlet holes 52 distributed in an array, and when the sample adding barrel 5 is positioned at the bottom end of the long barrel 4, the sample adding port 51 is just positioned inside the bottom end of the long barrel 4.
The sample is directly added from the sample adding port 51, the sample adding port 51 is blocked by the bottom end of the long tube 4, and bacterial liquid can flow in the liquid inlet 52 so as to bring the skin tissue into the culture medium, and the skin tissue cannot adhere to the inner wall of the culture tank 1, so that the culture effect is improved.
The middle of the top of the culture tank 1 is provided with an illumination component, the illumination component comprises a lamp tube, a lamp tube sleeve 6 and a long lamp tube 61, the long lamp tube 61 is arranged in the lamp tube sleeve 6, and the long lamp tube 61 is inserted into the lamp tube from the inside to enable the lamp tube sleeve 6 to be sleeved on the lamp tube in a rotating mode.
Culture light conditions, or sterilization light may be provided.
The outer wall of the temporary storage barrel 3 is provided with a sterilizing heating plate 34. After each sample sampling, the temporary storage barrel 3 can be sterilized by a sterilizing heating plate 34 to prevent bacteria from remaining in the temporary storage barrel.
An observation window is arranged on the outer wall of the temporary storage barrel 3. The method is convenient for observing the amount of the discharged bacterial liquid and stopping the discharge of the bacterial liquid in time.
The rotating shaft of the stirring motor 2 is provided with a mechanical sealing piece on the bottom wall of the culture tank 1. The tightness of the position of the rotating shaft is improved, and the mechanical sealing element is of a conventional structure.
The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.
Claims (6)
1. An anti-pollution bacterial culture device, characterized in that: including cultivate jar (1), application of sample subassembly, strain and release subassembly and aseptic flowing back mechanism, cultivate jar (1) bottom and be equipped with agitator motor (2) and vortex stirring rake (21), the pivot of agitator motor (2) is inserted in cultivate jar (1) bottom and is overlapped in the pivot and has vortex stirring rake (21), cultivate jar (1) bottom one side is equipped with fluid-discharge tube (12), cultivate jar (1) are equipped with liquid feeding pipe (13) in intermediate position one side of last half, cultivate jar (1) lower half outer wall a week and be equipped with heating plate (11), the application of sample subassembly includes long bobbin (4), bobbin lid (41), stretches into pole (42) and application of sample bucket (5), stretch into pole (42) top and fix on bobbin lid (41) inner roof, bottom fixing at application of sample bucket (5) top, application of sample bucket (5) are inserted and are inserted long bobbin (4) bottom and are exposed from long bobbin (4) top, strain and release subassembly includes release pipe (7), strain and release pipe (8) and release pipe (9) are equipped with heating plate (11) in the middle part of the upper and lower half of air filtering pipe (8) and release pipe (8) are cup jointed respectively, all be equipped with the manual valve on fluid-discharge tube (12), liquid feeding pipe (13) and air release valve pipe (9), aseptic flowing back mechanism is including the feed liquor pipe (31), drain pipe (32) and blast pipe (33) that set up on barrel of keeping in barrel (3) and the barrel, barrel (3) of keeping in is close to the bottom both sides and is equipped with feed liquor pipe (31) and drain pipe (32), barrel (3) top of keeping in is equipped with the blast pipe, feed liquor pipe (31) are inserted in culture tank (1) and are higher than vortex stirring rake (21).
2. The anti-pollution bacterial culture device according to claim 1, wherein: the sample adding barrel is characterized in that a sample adding port (51) is formed in one side of the upper half part of the sample adding barrel (5), a plurality of liquid inlet holes (52) distributed in an array are formed in the lower half part of the sample adding barrel, and when the sample adding barrel (5) is positioned at the bottom end of the long barrel (4), the sample adding port (51) is just positioned inside the bottom end of the long barrel (4).
3. The anti-pollution bacterial culture device according to claim 1, wherein: the middle of the top of the culture tank (1) is provided with an illumination assembly, the illumination assembly comprises a lamp tube, a lamp tube sleeve (6) and a long lamp tube (61), the long lamp tube (61) is arranged in the lamp tube sleeve (6), and the long lamp tube (61) is inserted into the lamp tube to sleeve the lamp tube sleeve (6) on the lamp tube.
4. The anti-pollution bacterial culture device according to claim 1, wherein: the outer wall of the temporary storage barrel (3) is provided with a sterilizing heating plate (34).
5. The anti-pollution bacterial culture device according to claim 1, wherein: an observation window is arranged on the outer wall of the temporary storage barrel (3).
6. The anti-pollution bacterial culture device according to claim 1, wherein: the rotating shaft of the stirring motor (2) is provided with a mechanical sealing piece on the bottom wall of the culture tank (1).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321286698.9U CN220056824U (en) | 2023-05-24 | 2023-05-24 | Anti-pollution bacterial culture device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321286698.9U CN220056824U (en) | 2023-05-24 | 2023-05-24 | Anti-pollution bacterial culture device |
Publications (1)
Publication Number | Publication Date |
---|---|
CN220056824U true CN220056824U (en) | 2023-11-21 |
Family
ID=88763170
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202321286698.9U Active CN220056824U (en) | 2023-05-24 | 2023-05-24 | Anti-pollution bacterial culture device |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN220056824U (en) |
-
2023
- 2023-05-24 CN CN202321286698.9U patent/CN220056824U/en active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104363972B (en) | It is centrifuged dynamic filter and utilizes its cell separation system | |
CN104379722B (en) | Disposable bioreactor and top plate and their manufacturing method | |
CZ298148B6 (en) | Disposable device for axenically culturing and harvesting cells and/or tissue, battery of such devices and corresponding method | |
CN103451151A (en) | Method for culturing human umbilical cord mesenchymal stem cells | |
CN103966092A (en) | Organ decellularization and circular fusion regeneration culture bottle | |
CN220056824U (en) | Anti-pollution bacterial culture device | |
CN208857307U (en) | Stem cell media bottle | |
CN207193305U (en) | Doughnut exchanger and doughnut switch type culture systems | |
CN115895887B (en) | Influenza vaccine production device and method and influenza vaccine | |
CN209652297U (en) | A kind of multidirectional air pressure drainage set | |
KR101135052B1 (en) | A bioreactor easy to sterilize and liquid cultivation methods using the same | |
CN111269816A (en) | Reproductive specimen collecting and culturing device and using method | |
CN208933384U (en) | A kind of culture tube | |
CN108977358B (en) | Closed bioreactor and cell culture method thereof | |
CN210065729U (en) | Microbial cultivation base partial shipment device | |
CN207498370U (en) | A kind of high security Fermentation by Photosynthetic Bacteria tank | |
CN208667686U (en) | A kind of fermented tea installation for fermenting | |
CN209967124U (en) | Culture medium sterility maintaining system | |
CN113832028A (en) | Full-automatic cell culture system and culture method | |
CN207330958U (en) | A kind of three-in-one bioreactor | |
CN220012639U (en) | Plant lactic acid bacteria inoculation jar | |
CN215365695U (en) | Large-scale fermentation tank with degerming function | |
CN211871992U (en) | Device for in vitro simulation screening of bacteria capable of degrading oxalic acid | |
CN216129609U (en) | Cell damage repair culture device | |
CN215799575U (en) | EM fungus culture immobilization fermenting installation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant |