CN220012667U - Backflow prevention buffer device suitable for bioreactor - Google Patents
Backflow prevention buffer device suitable for bioreactor Download PDFInfo
- Publication number
- CN220012667U CN220012667U CN202321080567.5U CN202321080567U CN220012667U CN 220012667 U CN220012667 U CN 220012667U CN 202321080567 U CN202321080567 U CN 202321080567U CN 220012667 U CN220012667 U CN 220012667U
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- pipe section
- bioreactor
- diaphragm valve
- sampling
- valve
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- 230000002265 prevention Effects 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 239000010865 sewage Substances 0.000 claims abstract description 23
- 238000005070 sampling Methods 0.000 claims description 37
- 239000012528 membrane Substances 0.000 claims description 10
- 239000011521 glass Substances 0.000 claims description 5
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000004113 cell culture Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 238000004062 sedimentation Methods 0.000 description 3
- 238000007599 discharging Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a backflow prevention buffer device suitable for a bioreactor, which is arranged between a sewage outlet and a sewage pipeline of the bioreactor, and comprises a buffer tank assembly, a hose, a liquid level sensing device, a diaphragm valve, a breather and a one-way valve, wherein the hose comprises a first pipe section and a second pipe section; the inlet of the buffer tank assembly is communicated with the sewage outlet of the bioreactor through a first pipe section, and the outlet of the buffer tank assembly is communicated with the sewage pipeline through a second pipe section; one end of the first pipe section, which is connected with the bioreactor, is provided with a liquid level sensing device, a diaphragm valve is arranged on the first pipe section, a one-way valve is arranged on the second pipe section, and a breather is arranged around an inlet of the buffer tank component. The liquid level sensing device, the diaphragm valve, the one-way valve and other parts are arranged on the hose, so that microorganisms in the sewage pipeline can be effectively prevented from flowing backwards to the bioreactor when liquid in the bioreactor tank is discharged.
Description
Technical Field
The utility model relates to the field of bioreactors, in particular to a backflow prevention buffer device suitable for a bioreactor.
Background
Compared with the traditional culture mode, the cell bioreactor technology has obvious advantages in cell culture, so that the cell bioreactor technology is widely applied to drug development and large-scale culture production, the cell bioreactor realizes automatic control in the cell culture process, can comprehensively monitor the cell culture process, ensures the controllability of production, ensures the stability of the cell culture condition control of the cell bioreactor, and simultaneously needs to realize the aim of sterility and no pollution in the cell culture process.
The sedimentation liquid change is realized by injecting sterile compressed air into the bioreactor to ensure that the bioreactor is pressurized to realize the discharge of the sedimentation supernatant, but the process can not accurately and effectively judge the pressure condition in the sewage because the liquid discharge pipeline is directly connected with the sewage pipeline, so that the liquid can be caused to flow backwards into the bioreactor in the discharge process of the sedimentation supernatant, thereby causing pollution of products and irrecoverable loss.
Disclosure of Invention
Aiming at the defects in the prior art, the utility model aims to provide a backflow prevention buffer device suitable for a bioreactor.
The backflow-preventing buffer device suitable for the bioreactor is arranged between a sewage outlet and a sewage pipeline of the bioreactor and comprises a buffer tank assembly, a hose, a liquid level sensing device, a diaphragm valve, a breather and a one-way valve, wherein the hose comprises a first pipe section and a second pipe section;
the inlet of the buffer tank assembly is communicated with the sewage outlet of the bioreactor through the first pipe section, and the outlet of the buffer tank assembly is communicated with the sewage outlet through the second pipe section;
the one end that first pipeline section connected bioreactor is provided with liquid level sensing device, be provided with the diaphragm valve on the first pipeline section, be provided with the check valve on the second pipeline section, be provided with the respirator around the import of buffer tank subassembly.
Preferably, the diaphragm valve comprises a first diaphragm valve and a second diaphragm valve, wherein the first diaphragm valve is arranged at one end of the first pipe section connected with the bioreactor, and the second diaphragm valve is arranged at one end of the first pipe section connected with the buffer tank assembly;
the first diaphragm valve is located downstream of the level sensing means.
Preferably, the bioreactor further comprises a pressure sensor, wherein the pressure sensor is arranged on the bioreactor and is electrically connected with the first diaphragm valve, and the second diaphragm valve is electrically connected with the liquid level sensing device.
Preferably, the liquid level sensing means comprises a tuning fork level gauge.
Preferably, the diaphragm valve comprises an actuated diaphragm valve.
Preferably, the surge tank assembly comprises a surge tank and a sight glass disposed on a side wall of the surge tank, and the breather is disposed on a top of the surge tank.
Preferably, the device further comprises a sampling assembly, wherein the sampling assembly is arranged on the first pipe section and is positioned between the first diaphragm valve and the second diaphragm valve.
Preferably, the sampling assembly comprises a sampling pipe section, a sampling three-way valve and a sampling bottle, wherein one end of the sampling pipe section is communicated with the first pipe section, the other end of the sampling pipe section is communicated with the sampling bottle, and the sampling three-way valve is arranged on the sampling pipe section.
Compared with the prior art, the utility model has the following beneficial effects:
the utility model has simple structure and convenient operation, adopts the technical means that the liquid level sensing device, the diaphragm valve, the one-way valve and other parts are arranged on the hose to form the backflow preventing buffer device suitable for the bioreactor, thereby effectively preventing microorganisms in the sewage drain pipeline from flowing back to the bioreactor in the discharging process of the settled supernatant in the bioreactor tank.
Drawings
Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
the figure shows:
buffer tank 8 of bioreactor 1
Viewing mirror 9 of liquid level sensing device 2
First diaphragm valve 3 respirator 10
Hose 4 one-way valve 11
Sampling three-way valve 5 sewage drain 12
Second diaphragm valve 6 pressure sensor 13
Sampling bottle 7
Detailed Description
The present utility model will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present utility model, but are not intended to limit the utility model in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present utility model.
The utility model discloses a backflow prevention buffer device suitable for a bioreactor, which is formed by arranging a liquid level sensing device, a diaphragm valve, a one-way valve and other parts on a hose, and aims to prevent microorganisms in a sewage pipeline from flowing backwards to the bioreactor in the discharging process of settled supernatant in a tank of the bioreactor.
According to the backflow prevention buffer device suitable for the bioreactor, as shown in fig. 1, the backflow prevention buffer device is arranged between a sewage outlet and a sewage pipeline 12 of the bioreactor 1 and comprises a buffer tank assembly, a hose 4, a liquid level sensing device 2, a diaphragm valve, a breather 10 and a one-way valve 11, wherein the hose 4 comprises a first pipe section and a second pipe section; the inlet of the buffer tank assembly is communicated with the sewage outlet of the bioreactor 1 through the first pipe section, and the outlet of the buffer tank assembly is communicated with the sewage pipeline 12 through the second pipe section; one end of the first pipe section connected with the bioreactor 1 is provided with a liquid level sensing device 2, a diaphragm valve is arranged on the first pipe section, a one-way valve 11 is arranged on the second pipe section, a breather 10 is arranged around the inlet of the buffer tank assembly, and the breather 10 plays a role in preventing backflow. The hose 4 adopts a high-temperature and high-pressure resistant pipeline, including a 316L pipeline, an Toyox hose and the like.
The membrane valve comprises a first membrane valve 3 and a second membrane valve 6, wherein the first membrane valve 3 is arranged at one end of the first pipe section connected with the bioreactor 1, and the second membrane valve 6 is arranged at one end of the first pipe section connected with the buffer tank component; the first diaphragm valve 3 is located downstream of the liquid level sensing means 2. The bioreactor further comprises a pressure sensor 13, wherein the pressure sensor 13 is arranged on the bioreactor 1, the pressure sensor 13 is electrically connected with the first diaphragm valve 3, and the second diaphragm valve 6 is electrically connected with the liquid level sensing device 2. Through the pressure sensor 13 is electrically connected with the first diaphragm valve 3, the scheme that the second diaphragm valve 6 is electrically connected with the liquid level sensing device 2 can accurately and effectively judge the pressure condition in the sewage pipeline, and accurately control the opening and closing of the diaphragm valve.
In a preferred embodiment of the utility model, the liquid level sensing means 2 comprises a tuning fork level gauge. The diaphragm valve includes an actuated diaphragm valve. The hose 4 is an eastern kex hose. The buffer tank assembly comprises a buffer tank 8 and a sight glass 9 arranged on the side wall of the buffer tank 8, the flow direction of liquid in the buffer tank 8 can be observed through the sight glass 9 to see whether the check valve 11 has a reflux phenomenon, and the respirator 10 is arranged on the top of the buffer tank 8.
In another preferred embodiment of the utility model, it further comprises a sampling assembly, which is arranged on the first pipe section and is located between the first diaphragm valve 3 and the second diaphragm valve 6; the sampling assembly comprises a sampling pipe section, a sampling three-way valve 5 and a sampling bottle 7, wherein one end of the sampling pipe section is communicated with the first pipe section, the other end of the sampling pipe section is communicated with the sampling bottle 7, and the sampling three-way valve 5 is arranged on the sampling pipe section.
The working principle of the utility model is as follows:
the first step, by pressurizing the bioreactor 1, automatically opening the first diaphragm valve 3 when the pressure sensor 13 senses that the pressure reaches a set value; secondly, after the liquid level sensing device 2 senses the liquid, automatically opening the associated second diaphragm valve 6 to start liquid discharge; thirdly, observing the flow direction of the liquid in the buffer tank 8 through the sight glass 9 to see whether the check valve 11 has a reflux phenomenon or not; the fourth part can be sampled by a sampling device consisting of a sampling three-way valve 5 and a sampling bottle 7 if on-line sampling is needed; and fifthly, when the liquid level sensing device 2 senses that no liquid exists, the first diaphragm valve 3 and the second diaphragm valve 6 are automatically closed.
In the description of the present utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
The foregoing describes specific embodiments of the present utility model. It is to be understood that the utility model is not limited to the particular embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without affecting the spirit of the utility model. The embodiments of the utility model and the features of the embodiments may be combined with each other arbitrarily without conflict.
Claims (8)
1. The backflow prevention buffer device suitable for the bioreactor is characterized by being arranged between a sewage outlet and a sewage pipeline (12) of the bioreactor (1) and comprising a buffer tank assembly, a hose (4), a liquid level sensing device (2), a diaphragm valve, a breather (10) and a one-way valve (11), wherein the hose (4) comprises a first pipe section and a second pipe section;
the inlet of the buffer tank assembly is communicated with the sewage outlet of the bioreactor (1) through the first pipe section, and the outlet of the buffer tank assembly is communicated with the sewage pipeline (12) through the second pipe section;
one end of the first pipe section, which is connected with the bioreactor (1), is provided with a liquid level sensing device (2), a diaphragm valve is arranged on the first pipe section, a one-way valve (11) is arranged on the second pipe section, and a respirator (10) is arranged around the inlet of the buffer tank component.
2. The backflow prevention buffer device suitable for a bioreactor according to claim 1, wherein the membrane valve comprises a first membrane valve (3) and a second membrane valve (6), the first membrane valve (3) is arranged at one end of a first pipe section connected with the bioreactor (1), and the second membrane valve (6) is arranged at one end of the first pipe section connected with a buffer tank assembly;
the first diaphragm valve (3) is located downstream of the liquid level sensing means (2).
3. Backflow prevention buffer device suitable for use in a bioreactor according to claim 2, further comprising a pressure sensor (13), the pressure sensor (13) being arranged on the bioreactor (1), the pressure sensor (13) being electrically connected to the first diaphragm valve (3), the second diaphragm valve (6) being electrically connected to the liquid level sensing means (2).
4. Backflow prevention buffer device suitable for use in a bioreactor according to claim 1, wherein the liquid level sensing device (2) comprises a tuning fork level gauge.
5. The backflow prevention buffer device for a bioreactor of claim 1 wherein the diaphragm valve comprises an actuated diaphragm valve.
6. The backflow prevention buffer device suitable for a bioreactor according to claim 1, wherein the buffer tank assembly comprises a buffer tank (8) and a sight glass (9) arranged on the side wall of the buffer tank (8), and the respirator (10) is arranged on the top of the buffer tank (8).
7. Backflow prevention buffer device suitable for use in a bioreactor according to claim 2, further comprising a sampling assembly arranged on the first tube section between the first diaphragm valve (3) and the second diaphragm valve (6).
8. The backflow prevention buffer device suitable for a bioreactor according to claim 7, wherein the sampling assembly comprises a sampling pipe section, a sampling three-way valve (5) and a sampling bottle (7), one end of the sampling pipe section is communicated with the first pipe section, the other end of the sampling pipe section is communicated with the sampling bottle (7), and the sampling three-way valve (5) is arranged on the sampling pipe section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321080567.5U CN220012667U (en) | 2023-05-06 | 2023-05-06 | Backflow prevention buffer device suitable for bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321080567.5U CN220012667U (en) | 2023-05-06 | 2023-05-06 | Backflow prevention buffer device suitable for bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220012667U true CN220012667U (en) | 2023-11-14 |
Family
ID=88685666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321080567.5U Active CN220012667U (en) | 2023-05-06 | 2023-05-06 | Backflow prevention buffer device suitable for bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220012667U (en) |
-
2023
- 2023-05-06 CN CN202321080567.5U patent/CN220012667U/en active Active
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