CN219950970U - Fluorescent quantitative PCR detection chip - Google Patents
Fluorescent quantitative PCR detection chip Download PDFInfo
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- CN219950970U CN219950970U CN202321403886.5U CN202321403886U CN219950970U CN 219950970 U CN219950970 U CN 219950970U CN 202321403886 U CN202321403886 U CN 202321403886U CN 219950970 U CN219950970 U CN 219950970U
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- chip carrier
- pcr detection
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- quantitative pcr
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- 238000001514 detection method Methods 0.000 title claims abstract description 50
- 238000003753 real-time PCR Methods 0.000 title claims abstract description 23
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 238000005070 sampling Methods 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 abstract description 4
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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Abstract
The utility model discloses a fluorescent quantitative PCR detection chip, which comprises: the chip carrier is fixedly connected with a transparent cover plate on the outer side of the chip carrier, the upper surface of the chip carrier is fixedly connected with a sealing paste, and the lower end surface of the chip carrier is fixedly connected with a negative pressure air bag; the chip carrier is internally provided with a sample sampling groove, the chip carrier is internally provided with a PCR detection mixture chamber, and the chip carrier is internally provided with a reaction chamber. Through the device, the real-time condition inside the chip carrier can be visually seen through the transparent cover plate, the device is opened through the sealing paste by the aid of the designed negative pressure air bag through the air pressure difference, and the sample injected into the sample sampling groove is effectively pressed into the PCR detection mixing chamber and the reaction chamber through the air pressure difference, so that the whole detection process is convenient and rapid, and the device is effectively isolated from the outside, and the sample is prevented from being polluted.
Description
Technical Field
The utility model relates to the technical field of PCR detection chips, in particular to a fluorescent quantitative PCR detection chip.
Background
The polymerase chain reaction (polymerase chain reaction, PCR) uses a piece of DNA as a template that is amplified to a sufficient amount in the presence of a DNA polymerase and nucleotide substrates for structural and functional analysis. The PCR detection method has extremely important significance in the aspects of rapidly diagnosing bacterial infectious diseases clinically and the like.
The PCR detection is an important detection means for rapidly diagnosing bacterial infectious diseases clinically, and compared with the conventional PCR detection method, the PCR detection method has the advantages of complex operation, high environmental requirement, easy pollution in the operation process, false positive of results, and influence on the accuracy of detection results. The Chinese patent discloses a PCR rapid detection chip and a general multichannel PCR rapid detection chip set, the application number of which is CN202121524157.6, samples are sampled and detected through a sampling groove and an internal reaction chamber, but the introduction of the samples and the isolation of the whole device from the external environment during use are insufficient to avoid the invasion of external pollution sources.
Disclosure of Invention
The utility model aims to at least solve one of the technical problems in the prior art, and provides a fluorescent quantitative PCR detection chip, which can visually see the real-time condition inside a chip carrier through a transparent cover plate, and can be opened through a sealing paste when the device is used through the designed negative pressure air bag by means of air pressure difference, and a sample injected into the sample sampling groove is effectively pressed into a PCR detection mixing chamber and a reaction chamber inside the sample sampling groove through the air pressure difference, so that the whole detection process is convenient and rapid, and the sample is effectively isolated from the outside, and the sample is prevented from being polluted.
The utility model also provides a fluorescent quantitative PCR detection chip, which comprises: the chip carrier is fixedly connected with a transparent cover plate on the outer side of the chip carrier, the upper surface of the chip carrier is fixedly connected with a sealing paste, and the lower end surface of the chip carrier is fixedly connected with a negative pressure air bag; the chip carrier is internally provided with a sample sampling groove, the chip carrier is internally provided with a PCR detection mixture chamber, and the chip carrier is internally provided with a reaction chamber.
According to the fluorescent quantitative PCR detection chip, the first channel is arranged in the chip carrier, and one end of the first channel is communicated with the sample sampling groove. So that the sample inside the sample collection well is effectively introduced into the first channel.
According to the fluorescent quantitative PCR detection chip, a micro-channel is arranged in the chip carrier, and one end of the micro-channel is communicated with the first channel. So that the sample effectively enters the interior of the microchannel through the first channel.
According to the fluorescent quantitative PCR detection chip, a second channel is arranged in the chip carrier, and one end of the second channel is communicated with the reaction chamber. So that the sample effectively enters the interior of the reaction chamber.
According to the fluorescent quantitative PCR detection chip provided by the utility model, the other end of the micro-channel is communicated with the PCR detection mixture chamber, and the interior of the PCR detection mixture chamber is communicated with the second channel. So that the sample is effectively mixed from the inside of the PCR detection mixture chamber and then effectively enters the reaction chamber.
According to the fluorescent quantitative PCR detection chip provided by the utility model, the reaction chamber is internally provided with the air passage, and one end of the air passage is communicated with the negative pressure air bag. So that the negative pressure air bag works to effectively introduce the sample into the reaction chamber.
Advantageous effects
1. Compared with the prior art, the fluorescent quantitative PCR detection chip can visually see the real-time condition inside the chip carrier through the transparent cover plate.
2. Compared with the prior art, the fluorescent quantitative PCR detection chip has the advantages that the device is opened through the sealing paste by the aid of the designed negative pressure air bag through the air pressure difference, and samples injected into the sample sampling groove are effectively pressed into the PCR detection mixing chamber and the reaction chamber through the air pressure difference, so that the whole detection process is convenient and rapid, and the device is effectively isolated from the outside, and the samples are prevented from being polluted.
Drawings
The utility model is further illustrated by the following figures and examples;
FIG. 1 is a diagram showing the overall structure of a fluorescent quantitative PCR detection chip according to the present utility model;
FIG. 2 is a diagram showing the internal structure of a chip carrier of a fluorescent quantitative PCR detection chip according to the present utility model;
FIG. 3 is a top view showing the internal structure of a chip carrier of a fluorescent quantitative PCR detection chip according to the present utility model.
Legend description:
1. a transparent cover plate; 2. sealing and sticking; 3. a chip carrier; 4. a negative pressure air bag; 5. a sample sampling tank; 6. a microchannel; 7. a PCR detection mixture chamber; 8. a reaction chamber; 9. an airway; 10. a first channel; 11. and a second channel.
Detailed Description
Reference will now be made in detail to the present embodiments of the present utility model, examples of which are illustrated in the accompanying drawings, wherein the accompanying drawings are used to supplement the description of the written description so that one can intuitively and intuitively understand each technical feature and overall technical scheme of the present utility model, but not to limit the scope of the present utility model.
Referring to fig. 1 to 3, an embodiment of the present utility model is a fluorescent quantitative PCR detection chip, which includes: the chip carrier 3 is used for detecting samples, and the outer side surface of the chip carrier 3 is fixedly connected with the transparent cover plate 1 for protection and convenient observation. The upper surface fixedly connected with seal subsides 2 of chip carrier 3 for it is sealed, the lower terminal surface fixedly connected with negative pressure gasbag 4 of chip carrier 3 provides the negative pressure condition, and the inside of chip carrier 3 is provided with sample sampling groove 5 for the sample, and the inside of chip carrier 3 is provided with PCR detection mixture room 7, mixes sample and mixture, and the inside of chip carrier 3 is provided with reaction chamber 8 for the reaction of sample.
The chip carrier 3 is internally provided with a first channel 10 for introducing a sample, one end of the first channel 10 being in communication with the sample sampling slot 5. The chip carrier 3 is provided with a micro channel 6 inside for introducing a sample, and the other end of the micro channel 6 communicates with a PCR detection mixture chamber 7, and the inside of the PCR detection mixture chamber 7 communicates with a second channel 11. One end of the microchannel 6 communicates with the first channel 10. The chip carrier 3 is provided with a second channel 11 inside, one end of the second channel 11 being in communication with the reaction chamber 8. An air passage 9 is arranged in the reaction chamber 8, and one end of the air passage 9 is communicated with the negative pressure air bag 4.
Working principle: when the sample detecting device is used, the sealing paste 2 is punctured to inject a sample into the sample sampling groove 5, at the moment, the negative pressure air bag 4 starts to work, the sample in the sample sampling groove 5 is led into the first channel 10 and enters the micro-channel 6 from the first channel 10, the sample enters the PCR detecting mixture chamber 7 after passing through the micro-channel 6, and after being fully mixed with the mixture, the sample is led out from the second channel 11 and enters the reaction chamber 8 to react, and the reaction result is obtained. The reaction conditions and reaction results inside the chip carrier 3 are easily observed through the transparent cover plate 1.
The embodiments of the present utility model have been described in detail with reference to the accompanying drawings, but the present utility model is not limited to the above embodiments, and various changes can be made within the knowledge of one of ordinary skill in the art without departing from the spirit of the present utility model.
Claims (6)
1. A fluorescent quantitative PCR detection chip, comprising:
the chip carrier (3), the outer side of the chip carrier (3) is fixedly connected with a transparent cover plate (1), the upper surface of the chip carrier (3) is fixedly connected with a sealing patch (2), and the lower end surface of the chip carrier (3) is fixedly connected with a negative pressure air bag (4);
the chip carrier (3) is internally provided with a sample sampling groove (5), the chip carrier (3) is internally provided with a PCR detection mixture chamber (7), and the chip carrier (3) is internally provided with a reaction chamber (8).
2. The fluorescent quantitative PCR detection chip as set forth in claim 1, wherein a first channel (10) is provided inside the chip carrier (3), and one end of the first channel (10) is communicated with the sample sampling groove (5).
3. The fluorescent quantitative PCR detection chip as set forth in claim 1, wherein a microchannel (6) is provided inside the chip carrier (3), and one end of the microchannel (6) is in communication with the first channel (10).
4. The fluorescent quantitative PCR detection chip as set forth in claim 1, wherein a second channel (11) is provided inside the chip carrier (3), and one end of the second channel (11) is in communication with the reaction chamber (8).
5. A fluorescent quantitative PCR detection chip according to claim 3, characterized in that the other end of the microchannel (6) is in communication with a PCR detection mixture chamber (7), the interior of the PCR detection mixture chamber (7) being in communication with the second channel (11).
6. The fluorescence quantitative PCR detection chip according to claim 1, wherein an air passage (9) is arranged in the reaction chamber (8), and one end of the air passage (9) is communicated with the negative pressure air bag (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321403886.5U CN219950970U (en) | 2023-06-05 | 2023-06-05 | Fluorescent quantitative PCR detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321403886.5U CN219950970U (en) | 2023-06-05 | 2023-06-05 | Fluorescent quantitative PCR detection chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219950970U true CN219950970U (en) | 2023-11-03 |
Family
ID=88540920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321403886.5U Active CN219950970U (en) | 2023-06-05 | 2023-06-05 | Fluorescent quantitative PCR detection chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219950970U (en) |
-
2023
- 2023-06-05 CN CN202321403886.5U patent/CN219950970U/en active Active
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