CN219870990U - Biological sample concentration detector based on micro-fluidic chip - Google Patents
Biological sample concentration detector based on micro-fluidic chip Download PDFInfo
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- CN219870990U CN219870990U CN202320822821.8U CN202320822821U CN219870990U CN 219870990 U CN219870990 U CN 219870990U CN 202320822821 U CN202320822821 U CN 202320822821U CN 219870990 U CN219870990 U CN 219870990U
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Abstract
The utility model relates to the technical field of biological detection, in particular to a biological sample concentration detector based on a microfluidic chip, which comprises a base, wherein a light source component is arranged at the top of the base, a light filtering piece is arranged at the position close to the light source component, a light path collimation and light splitting piece is arranged at the top of the base, a detection chip storage bracket is arranged at the position close to the light path collimation and light splitting piece on the outer wall of the base, a concentration detection chip is arranged in the detection chip storage bracket, and the concentration detection chip based on the microfluidic chip is designed.
Description
Technical Field
The utility model relates to the technical field of biological detection, in particular to a biological sample concentration detector based on a microfluidic chip.
Background
The absorbance detection, because of some groups of different molecules absorb the radiant light, the electron energy level transition occurs to generate the absorption spectrum, each substance has its own fixed absorption spectrum, when the concentration of the substance is different, the absorbance of some characteristic wavelength on the absorption spectrum is also different, from the determination of the absorbance or transmittance of the substance, the content of the substance is judged, latex microsphere is a kind of micro-nano level particle, usually made of high molecular material such as polystyrene, when coupled with the antibody, it mixes with the substance to be detected, it will gather, thus influence the absorbance of the solution under the specific wavelength, the absorbance change is positively correlated with the concentration of the substance to be detected, the microfluidic chip refers to the biological or chemical laboratory constructed on a chip of several square millimeters or several square centimeters, the processes of sample injection, reaction, detection, etc. related in the biological or chemical field are integrated, the characteristics of high precision, small sample injection quantity, high integration level, and easy automation.
The existing spectrophotometer has the problems that the volume is large, the operation flux is low, the high flux automation is not easy to realize, the use cost of single consumable is high, and in the process, the reference sample contrast detection operation is required to be complicated.
Therefore, a biological sample concentration detector based on a microfluidic chip is needed to solve the problems set forth in the background art.
Disclosure of Invention
The utility model aims to provide a biological sample concentration detector based on a microfluidic chip, so as to solve the problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
the utility model provides a biological sample concentration detector based on micro-fluidic chip, includes the base, the top of base is provided with the light source subassembly, the top of base and be provided with the optical filter in being close to light source subassembly position department, the top of base and be provided with light path collimation beam splitter in being close to optical filter position department, the outer wall of base and be provided with the detection chip storage support in being close to light path collimation beam splitter position department, the inside of detection chip storage support is provided with concentration detection chip, the top of detection chip storage support is provided with the automatic unloading subassembly of going up of consumptive material, the top of consumptive material automatic unloading subassembly is provided with rotary motion servo, the outer wall of base and be provided with the chip top machine in the below of detection chip storage support, the top of base and be provided with the detection processor in the back of light source subassembly;
the concentration detection chip comprises a chip body which is in sliding connection with the inside of the detection chip storage bracket, an exhaust liquid discharge hole is formed in the top of the chip body, a detection hole site is formed in the outer wall of the chip body and close to the position of the exhaust liquid discharge hole, a mixing pipe is arranged on the outer wall of the detection hole site, a sample filling hole is formed in the outer wall of the chip body and close to the position of the mixing pipe, a chip positioning hole is formed in the upper part of the center of the outer wall of the chip body, a centrifugal rotating shaft hole is formed in the center of the outer wall of the chip body, an operation positioning groove is formed in the inside of the chip body and close to the position of the centrifugal rotating shaft hole, and an operation clamping groove is formed in the outer wall of the chip body and close to the position of the operation positioning groove;
the automatic chip support shell that goes up of unloading subassembly of consumptive material includes fixed connection at the base top, the rotating electrical machines is installed at the top of chip support shell, the inside of chip support shell and be provided with the chip tray in the below of rotating electrical machines, the below of chip support shell is provided with the chip lift, the back of chip support shell is provided with drive mechanism, the receiver optical path has been seted up at the top of chip support shell, the inside of chip support shell and the top at the chip tray are provided with the rotation axis sensor of returning to zero, the inner wall of chip support shell is provided with the structure slide rail, the inside of chip support shell is provided with the chip revolving stage pivot, the inside of chip support shell and be provided with the rotatory centrifugal machine of chip directly over the chip revolving stage pivot, the inside of chip support shell and the below of chip rotatory centrifugal machine of chip are provided with the chip locating piece.
As a preferable scheme of the utility model, the light source assembly comprises a light source chamber shell fixedly connected to the top of the base, a light source micropore is formed in the inner wall of the light source chamber shell, a light source lens mounting position is arranged on the outer wall of the light source chamber shell, a light source piece is mounted on the inner wall of the light source chamber shell, and a radiator is mounted on the outer wall of the light source chamber shell.
As a preferred embodiment of the present utility model, the light source member is made of a combination of deuterium lamps and white light lamps, and the light source chamber housing is made of an aluminum alloy.
As a preferable scheme of the utility model, the exhaust and liquid discharge holes, the detection hole sites, the mixing pipes and the sample filling holes are all provided with a plurality of groups, and the chip body is of a circular structure and the two sides of the chip body are subjected to edge cutting treatment.
As a preferable scheme of the utility model, the mixing tube is of a serpentine structure design, the chip positioning holes are of a triangular structure design, and the chip body is a microfluidic chip.
As a preferable scheme of the utility model, a plurality of groups of light holes are formed in the chip tray, and the chip tray is connected with the structural slide rail in a sliding manner.
Compared with the prior art, the utility model has the beneficial effects that:
1. according to the biological sample concentration detector based on the microfluidic chip, a concentration detection chip and a consumable automatic feeding and discharging component in the device are utilized for detection, samples are filled into the concentration detection chip, the concentration detection chip is positioned, clamped, transported and fed, a chip rotary centrifugal driver drives the concentration detection chip to rotate at a low speed through a chip rotary table rotating shaft, the samples flow into a mixing pipe port and flow in a serpentine manner in the mixing pipe, bubbles in the samples are discharged through an exhaust liquid discharge hole, concentration detection of a comparison reference sample and concentration detection of a target sample are completed, the concentration detection chip is automatically fed out, and parallel multichannel biological chips are used as a basis to operate carriers, so that the online parallel operation of tens of samples, different spectral bands, multiple types of samples or different detection items is realized, the utilization rate of the used space in the detection process is greatly improved, the use amount of the samples and the reagents is reduced, the reaction time is shortened, the detection reaction time is optimized, the sample operations such as feeding and discharging, light-shielding protection and sample feeding are realized, the compact structure design of the device is convenient for the integration and layout of an automatic production line, the existing volume-based on the parallel multichannel biological sample detector is easy to realize, the problem that the current single-sample detection operation has high cost and the comparison sample has high cost.
Drawings
FIG. 1 is a schematic perspective view of the present utility model;
FIG. 2 is a schematic perspective view of a light source assembly according to the present utility model;
FIG. 3 is a front view of a concentration detection chip of the present utility model;
FIG. 4 is a side sectional view of a concentration detection chip of the present utility model;
FIG. 5 is a schematic diagram of a three-dimensional structure of an automatic consumable feeding and discharging assembly according to the present utility model;
FIG. 6 is a schematic view of an unloading perspective structure of the chip tray of the present utility model;
FIG. 7 is a perspective front sectional view of the consumable automatic loading and unloading assembly of the present utility model;
FIG. 8 is a basic operation flow chart of the consumable automatic loading and unloading assembly of the utility model;
FIG. 9 is a flow chart showing basic operation of the concentration detection chip of the present utility model.
In the figure: 1. a base; 2. a light source assembly; 3. a light filter; 4. the light path collimates the beam splitter; 5. a detection chip storage bracket; 6. a concentration detection chip; 7. consumable automatic feeding and discharging assembly; 8. a rotary motion servo; 9. a chip top machine; 10. a detection processor; 201. a light source chamber housing; 202. a light source micropore; 203. a light source lens mounting position; 204. a light source member; 205. a heat sink; 601. a chip body; 602. an exhaust and liquid discharge hole; 603. detecting hole sites; 604. mixing uniformly; 605. a sample filling hole; 606. a chip positioning hole; 607. centrifugal rotation shaft hole; 608. operating the positioning groove; 609. operating the clamping groove; 701. a chip support casing; 702. a rotating electric machine; 703. a chip tray; 704. a chip lifter; 705. a transmission mechanism; 706. a receiver optical path; 707. a rotation axis return-to-zero sensor; 708. a structural slide rail; 709. a chip turntable rotating shaft; 710. a chip rotation centrifugal driver; 711. the chip clamps the positioning block.
Detailed Description
The technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments, and all other embodiments obtained by those skilled in the art without making any inventive effort based on the embodiments of the present utility model are within the scope of protection of the present utility model.
In order that the utility model may be readily understood, several embodiments of the utility model will be described more fully hereinafter with reference to the accompanying drawings, in which, however, the utility model may be embodied in many different forms and is not limited to the embodiments described herein, but instead is provided for the purpose of providing a more thorough and complete disclosure of the utility model.
It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may also be present, and when an element is referred to as being "connected" to the other element, it may be directly connected to the other element or intervening elements may also be present, the terms "vertical", "horizontal", "left", "right" and the like are used herein for the purpose of illustration only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs, and the terms used herein in this description of the utility model are for the purpose of describing particular embodiments only and are not intended to be limiting of the utility model, with the term "and/or" as used herein including any and all combinations of one or more of the associated listed items.
Referring to fig. 1-9, the present utility model provides a technical solution:
the utility model provides a biological sample concentration detector based on micro-fluidic chip, including base 1, the top of base 1 is provided with light source subassembly 2, the top of base 1 and be provided with filter 3 near light source subassembly 2 position department, the top of base 1 and be provided with light path collimation beam splitter 4 near filter 3 position department, the outer wall of base 1 and be provided with detection chip storage support 5 near light path collimation beam splitter 4 position department, the inside of detection chip storage support 5 is provided with concentration detection chip 6, the top of detection chip storage support 5 is provided with consumable automation feeding and discharging subassembly 7, the top of consumable automation feeding and discharging subassembly 7 is provided with rotary motion servo 8, the outer wall of base 1 and be provided with chip top machine 9 below detection chip storage support 5, the top of base 1 and be provided with detection processor 10 at the back of light source subassembly 2;
in this embodiment, referring to fig. 3, 4 and 9, the concentration detection chip 6 includes a chip body 601 slidingly connected inside the detection chip storage rack 5, an exhaust and drain hole 602 is provided at the top of the chip body 601, a detection hole site 603 is provided on the outer wall of the chip body 601 at a position close to the exhaust and drain hole 602, a mixing pipe 604 is provided on the outer wall of the detection hole site 603, a sample filling hole 605 is provided on the outer wall of the chip body 601 at a position close to the mixing pipe 604, a chip positioning hole 606 is provided above the center of the outer wall of the chip body 601, a centrifugal rotation shaft hole 607 is provided on the center of the outer wall of the chip body 601, an operation positioning groove 608 is provided inside the chip body 601 at a position close to the centrifugal rotation shaft hole 607, and an operation clamping groove 609 is provided on the outer wall of the chip body 601 at a position close to the operation positioning groove 608;
the exhaust and liquid discharge holes 602, the detection hole sites 603, the mixing pipes 604 and the sample filling holes 605 are all provided with a plurality of groups, the chip body 601 is of a circular structure, the two sides of the chip body are subjected to edge cutting treatment, the mixing pipes 604 are of a serpentine structure design, the chip positioning holes 606 are of a triangular structure design, and the chip body 601 is a microfluidic chip;
in this embodiment, referring to fig. 5, 6, 7 and 8, the consumable automatic feeding and discharging assembly 7 includes a chip support housing 701 fixedly connected to the top of the base 1, a rotating motor 702 is mounted on the top of the chip support housing 701, a chip tray 703 is provided inside the chip support housing 701 and below the rotating motor 702, a chip lifter 704 is provided below the chip support housing 701, a transmission mechanism 705 is provided on the back surface of the chip support housing 701, a receiver optical path 706 is provided on the top of the chip support housing 701, a rotary shaft return-to-zero sensor is provided inside the chip support housing 701 and above the chip tray 703, a structural slide rail 708 is provided on the inner wall of the chip support housing 701, a chip turntable rotary shaft 709 is provided inside the chip support housing 701 and directly above the chip turntable rotary shaft 709, a chip rotary centrifugal driver 710 is provided inside the chip support housing 711 and a chip positioning block is provided below the chip rotary centrifugal driver 710;
wherein, the chip tray 703 is provided with a plurality of groups of light holes, and the connection mode between the chip tray 703 and the structure sliding rail 708 is sliding connection;
in this embodiment, referring to fig. 1 and 2, the light source assembly 2 includes a light source chamber housing 201 fixedly connected to the top of the base 1, a light source micro-hole 202 is opened in the inner wall of the light source chamber housing 201, a light source lens mounting position 203 is provided on the outer wall of the light source chamber housing 201, a light source member 204 is mounted on the inner wall of the light source chamber housing 201, and a heat sink 205 is mounted on the outer wall of the light source chamber housing 201;
wherein the light source member 204 is made of a combination of deuterium lamps and white light lamps, and the light source chamber housing 201 is made of an aluminum alloy.
The working flow of the utility model is as follows: when the biological sample concentration detector based on the microfluidic chip designed by the scheme is operated, 0.2-5ul of pre-configured gradient concentration calibration liquid is taken and added into a sample filling hole 605, a solution to be detected is taken and added into the sample filling hole 605, the liquid is suspended in a mixing pipe 604 at the moment, a chip body 601 is manually placed on a consumable automatic feeding and discharging assembly 7 of the detector according to a chip positioning hole 606, after the feeding is completed, the chip body is moved into the detector under the action of a chip tray 703, a transmission mechanism 705 and a structure sliding rail 708 in the consumable automatic feeding and discharging assembly 7, in a darkroom environment, the detector is started, a light source part 204 is started, ultraviolet light is excited by a deuterium lamp in the light source part 204, enters a light path collimation and light splitting part 4 and a light filtering part 3 through a light source micropore 202 of a light source chamber shell 201 to perform optical convergence and light splitting, the ultraviolet light source generates light filtering through a light filter to obtain a light source of a target wave band, irradiates the light source on a detector in a detection processor 10, converts an optical signal into an electric signal, stores the electric signal, records the measuring range as absorbance 0%, synchronously calculates a subsequent operation compensation value, and lifts up a chip body 601 by a chip top machine 9, the chip body 601 can be tightly combined with a chip rotary centrifugal driver 710, the chip body 601 starts high-speed centrifugal rotation under the driving of the chip rotary centrifugal driver 710, the liquid suspended in a mixing tube 604 flows to a detection hole site 603 and starts to read a disc, records absorbance, after centrifugation, the chip body 601 returns to the original point, the detection hole site 603 and a receiver optical path 706 are concentric, then rotates according to a specific angle, the instrument reads the disc according to the number of selected items, records absorbance in an air state, obtains absorbance of the solution through a built-in program, and fitting a reference curve according to the calibration liquid.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a biological sample concentration detector based on micro-fluidic chip, includes base (1), its characterized in that: the automatic light source detection device comprises a base (1), and is characterized in that a light source component (2) is arranged at the top of the base (1), a light filtering piece (3) is arranged at the position close to the light source component (2), a light path collimation and light splitting piece (4) is arranged at the top of the base (1) and at the position close to the light filtering piece (3), a detection chip storage bracket (5) is arranged at the outer wall of the base (1) and at the position close to the light path collimation and light splitting piece (4), a concentration detection chip (6) is arranged in the detection chip storage bracket (5), a consumable automatic feeding and discharging component (7) is arranged at the top of the detection chip storage bracket (5), a rotary motion servo machine (8) is arranged at the top of the consumable automatic feeding and discharging component (7), a chip jacking machine (9) is arranged at the outer wall of the base (1) and below the detection chip storage bracket (5), and a detection processor (10) is arranged at the top of the base (1) and at the back of the light source component (2);
the concentration detection chip (6) comprises a chip body (601) which is connected inside a detection chip storage bracket (5) in a sliding mode, an exhaust liquid discharge hole (602) is formed in the top of the chip body (601), a detection hole site (603) is formed in the outer wall of the chip body (601) and at a position close to the exhaust liquid discharge hole (602), a mixing pipe (604) is arranged on the outer wall of the detection hole site (603), a sample filling hole (605) is formed in the outer wall of the chip body (601) and at a position close to the mixing pipe (604), a chip positioning hole (606) is formed in the upper portion of the center of the outer wall of the chip body (601), a centrifugal rotating shaft hole (607) is formed in the center of the outer wall of the chip body (601), an operation positioning groove (608) is formed in the inner portion of the chip body (601) and at a position close to the centrifugal rotating shaft hole (607), and an operation clamping groove (609) is formed in the outer wall of the chip body (601) and at a position close to the operation positioning groove (608).
The automatic chip support blanking assembly (701) of going up of consumptive material includes fixed connection at chip support shell (701) at base (1) top, rotating electrical machines (702) are installed at the top of chip support shell (701), the inside of chip support shell (701) and be provided with chip tray (703) in the below of rotating electrical machines (702), the below of chip support shell (701) is provided with chip lift (704), the back of chip support shell (701) is provided with drive mechanism (705), receiver optical path (706) has been seted up at the top of chip support shell (701), the inside of chip support shell (701) and be provided with rotation axis sensor (707) that return to zero in the top of chip tray (703), the inner wall of chip support shell (701) is provided with structure slide rail (708), the inside of chip support shell (701) is provided with chip revolving stage pivot (709), the inside of chip support shell (701) and be provided with chip centrifuge spin-up machine (710) directly over chip support shell (709), chip support (701) inside spin-down chucking machine (710) is provided with chip centrifuge spin-down location piece (710).
2. The microfluidic chip-based biological sample concentration detector according to claim 1, wherein: the light source assembly (2) comprises a light source chamber shell (201) fixedly connected to the top of the base (1), a light source micropore (202) is formed in the inner wall of the light source chamber shell (201), a light source lens mounting position (203) is arranged on the outer wall of the light source chamber shell (201), a light source piece (204) is mounted on the inner wall of the light source chamber shell (201), and a radiator (205) is mounted on the outer wall of the light source chamber shell (201).
3. The microfluidic chip-based biological sample concentration detector according to claim 2, wherein: the light source member (204) is made of a combination of deuterium lamps and white light lamps, and the light source chamber housing (201) is made of an aluminum alloy.
4. The microfluidic chip-based biological sample concentration detector according to claim 1, wherein: the chip is characterized in that the exhaust liquid discharge holes (602), the detection hole sites (603), the mixing pipes (604) and the sample filling holes (605) are all provided with a plurality of groups, the chip body (601) is of a circular structure, and the two sides of the chip body are subjected to edge cutting treatment, so that the operation of automatic equipment is facilitated.
5. The microfluidic chip-based biological sample concentration detector according to claim 1, wherein: the mixing tube (604) is of a serpentine structure design, the chip positioning holes (606) are of a triangular structure design, and the chip body (601) is a microfluidic chip.
6. The microfluidic chip-based biological sample concentration detector according to claim 1, wherein: the chip tray (703) is provided with a plurality of groups of light holes, and the chip tray (703) is connected with the structural slide rail (708) in a sliding manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320822821.8U CN219870990U (en) | 2023-04-14 | 2023-04-14 | Biological sample concentration detector based on micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320822821.8U CN219870990U (en) | 2023-04-14 | 2023-04-14 | Biological sample concentration detector based on micro-fluidic chip |
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| Publication Number | Publication Date |
|---|---|
| CN219870990U true CN219870990U (en) | 2023-10-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320822821.8U Active CN219870990U (en) | 2023-04-14 | 2023-04-14 | Biological sample concentration detector based on micro-fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219870990U (en) |
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2023
- 2023-04-14 CN CN202320822821.8U patent/CN219870990U/en active Active
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