CN219772144U - Cell line culture and amplification device - Google Patents
Cell line culture and amplification device Download PDFInfo
- Publication number
- CN219772144U CN219772144U CN202321287565.3U CN202321287565U CN219772144U CN 219772144 U CN219772144 U CN 219772144U CN 202321287565 U CN202321287565 U CN 202321287565U CN 219772144 U CN219772144 U CN 219772144U
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- pipe
- light
- tube
- light source
- transmitting
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- 230000003321 amplification Effects 0.000 title claims abstract description 13
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 13
- 239000000725 suspension Substances 0.000 claims abstract description 18
- 241000276425 Xiphophorus maculatus Species 0.000 claims abstract description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 230000008878 coupling Effects 0.000 abstract description 3
- 238000010168 coupling process Methods 0.000 abstract description 3
- 238000005859 coupling reaction Methods 0.000 abstract description 3
- 238000010276 construction Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 8
- 230000006870 function Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell line culture and amplification device. The device adopts a closed container as a culture space, and a structure of combining a plate-shaped light source and a columnar light source is adopted in the container, so that sufficient light energy supply is realized. In the aspect of construction, the light-transmitting sealed cavity is arranged on the support panel, the illuminating lamp is arranged in the sealed cavity, the wire connector is connected with the illuminating lamp, and the wire connector sequentially penetrates out through the annular tube and the suspension tube and is connected with the power supply; the ring support frame is connected on the bottom sprag frame, and fixedly connected with hangs the pipe in the culture tank, hangs the pipe and runs through the culture tank top, and ring form union coupling is in hanging the pipe bottom in the suspension, and platy light source fixed connection is provided with the port between ring support frame and ring form pipe in the culture tank bottom, in port fixedly connected with light-transmitting pipe, and the light-transmitting pipe is located the culture tank, and the light-transmitting pipe top is sealed, installs the light source in the light-transmitting pipe. The light source is closed, and the light energy supply rate is higher.
Description
Technical Field
The utility model relates to the technical field of bioengineering, in particular to a cell line culture and amplification device.
Background
Cell culture refers to a method of simulating in vitro an in vivo environment (sterile, suitable temperature, ph, certain nutritional conditions, etc.) to survive, grow, reproduce and maintain major structures and functions. Cell culture is the basic work of biological product production, and at present, the culture process is carried out in a closed reactor, and the performance of the reactor has a direct influence on culture efficiency. Unlike animal cells, plant cells need to be cultured under conditions such as proper temperature, pH, and nutrition, and also need to provide sufficient light and necessary growth factors. Currently, a general culture apparatus has yet to be improved in terms of light energy supply rate.
Disclosure of Invention
The utility model aims at overcoming the technical defects of the prior art, and provides a cell line culture and amplification device for solving the technical problem that a common culture device is to be improved in terms of light energy supply rate.
In order to achieve the technical purpose, the utility model adopts the following technical scheme:
a cell line culture and amplification device comprises a culture tank, an input pipe, an output pipe, a bottom support frame, a circular ring support frame, a suspension pipe, a circular ring pipe, a plate-shaped light source, a port and a light-transmitting pipe; the plate-shaped light source comprises a support panel, a light-transmitting sealed cavity is arranged on the support panel, the illuminating lamp is arranged in the sealed cavity, the wire connector is connected with the illuminating lamp, and the wire connector sequentially penetrates out through the annular tube and the suspension tube and is connected with the power supply; the input tube and the output tube are located the top and the bottom of cultivateing the jar respectively, the bottom sprag frame is fixed in the cultivate jar, the ring support frame is connected on the bottom sprag frame, fixedly connected with suspender in midair in cultivate jar, suspend the pipe and run through cultivate jar top, ring form union coupling is in suspending the pipe bottom in midair, platy light source fixed connection is between ring support frame and ring form pipe, be provided with the port in cultivate jar bottom, at port fixedly connected with light-transmitting tube, the light-transmitting tube is located cultivate jar, the light-transmitting tube top seals, install the light source in the light-transmitting tube.
Preferably, a lamp tube is installed in the light-transmitting tube, and a line of the lamp tube passes through the port and is connected with a power supply.
Preferably, an air supply line is connected to the bottom of the culture tank.
Preferably, a feed tube and an observation window are respectively connected to the top of the culture tank.
Preferably, a temperature sensor, a pH sensor, and a dissolved oxygen sensor are mounted in the culture tank.
Preferably, the culture tank is mounted on a support structure, and casters are mounted on a bottom plate of the support structure.
Preferably, the sealed cavities are strip-shaped and are uniformly distributed on the plate-shaped light source.
The utility model discloses a cell line culture and amplification device. The device adopts a closed container as a culture space, and a structure of combining a plate-shaped light source and a columnar light source is adopted in the container, so that sufficient light energy supply is realized. In the aspect of construction, the light-transmitting sealed cavity is arranged on the support panel, the illuminating lamp is arranged in the sealed cavity, the wire connector is connected with the illuminating lamp, and the wire connector sequentially penetrates out through the annular tube and the suspension tube and is connected with the power supply; the ring support frame is connected on the bottom sprag frame, and fixedly connected with hangs the pipe in the culture tank, hangs the pipe and runs through the culture tank top, and ring form union coupling is in hanging the pipe bottom in the suspension, and platy light source fixed connection is provided with the port between ring support frame and ring form pipe in the culture tank bottom, in port fixedly connected with light-transmitting pipe, and the light-transmitting pipe is located the culture tank, and the light-transmitting pipe top is sealed, installs the light source in the light-transmitting pipe. The light source is closed, and the light energy supply rate is higher.
Drawings
FIG. 1 is a half cross-sectional view of the present utility model;
fig. 2 is a perspective view of a plate-like light source;
FIG. 3 is a cross-sectional view of a plate-like light source;
wherein:
1. culture tank 2, input pipe 3, output pipe 4, bottom support frame
5. Circular ring support 6, hanging tube 7, circular ring tube 8 and plate-shaped light source
9. Port 10, light-transmitting tube 11, support panel 12, sealed cavity
13. Lighting lamp 14, wire connector.
Detailed Description
Hereinafter, embodiments of the present utility model will be described in detail. In order to avoid unnecessary detail, well-known structures or functions will not be described in detail in the following embodiments. Approximating language, as used in the following examples, may be applied to create a quantitative representation that could permissibly vary without resulting in a change in the basic function. Unless defined otherwise, technical and scientific terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs.
Example 1
The cell line culture and amplification device comprises a culture tank 1, an input pipe 2, an output pipe 3, a bottom support frame 4, a circular ring support frame 5, a suspension pipe 6, a circular ring pipe 7, a plate-shaped light source 8, a port 9 and a light transmission pipe 10, wherein the input pipe 2 is connected with the bottom support frame; the plate-shaped light source 8 comprises a support panel 11, a light-transmitting sealed cavity 12 is arranged on the support panel 11, a lighting lamp 13 is arranged in the sealed cavity 12, a wire connector 14 is connected with the lighting lamp 13, and the wire connector 14 sequentially penetrates through the annular tube 7 and the suspension tube 6 and is connected with a power supply; the input tube 2 and the output tube 3 are respectively positioned at the top end and the bottom end of the culture tank 1, the bottom support frame 4 is fixed in the culture tank 1, the circular support frame 5 is connected on the bottom support frame 4, the suspension tube 6 is fixedly connected in the culture tank 1, the suspension tube 6 penetrates through the top of the culture tank 1, the circular tube 7 is connected at the bottom end of the suspension tube 6, the platy light source 8 is fixedly connected between the circular support frame 5 and the circular tube 7, the port 9 is arranged at the bottom of the culture tank 1, the light-transmitting tube 10 is fixedly connected at the port 9, the light-transmitting tube 10 is positioned in the culture tank 1, the top end of the light-transmitting tube 10 is closed, and the light source is arranged in the light-transmitting tube 10.
Example 2
The cell line culture and amplification device comprises a culture tank 1, an input pipe 2, an output pipe 3, a bottom support frame 4, a circular ring support frame 5, a suspension pipe 6, a circular ring pipe 7, a plate-shaped light source 8, a port 9 and a light transmission pipe 10, wherein the input pipe 2 is connected with the bottom support frame; the plate-shaped light source 8 comprises a support panel 11, a light-transmitting sealed cavity 12 is arranged on the support panel 11, a lighting lamp 13 is arranged in the sealed cavity 12, a wire connector 14 is connected with the lighting lamp 13, and the wire connector 14 sequentially penetrates through the annular tube 7 and the suspension tube 6 and is connected with a power supply; the input tube 2 and the output tube 3 are respectively positioned at the top end and the bottom end of the culture tank 1, the bottom support frame 4 is fixed in the culture tank 1, the circular support frame 5 is connected on the bottom support frame 4, the suspension tube 6 is fixedly connected in the culture tank 1, the suspension tube 6 penetrates through the top of the culture tank 1, the circular tube 7 is connected at the bottom end of the suspension tube 6, the platy light source 8 is fixedly connected between the circular support frame 5 and the circular tube 7, the port 9 is arranged at the bottom of the culture tank 1, the light-transmitting tube 10 is fixedly connected at the port 9, the light-transmitting tube 10 is positioned in the culture tank 1, the top end of the light-transmitting tube 10 is closed, and the light source is arranged in the light-transmitting tube 10. A lamp tube is installed in the light-transmitting tube 10, and a line of the lamp tube passes out through the port 9 and is connected with a power supply. The bottom end of the culture tank 1 is connected with an air supply pipeline. The top of the culture tank 1 is respectively connected with a feed supplement pipe and an observation window. A temperature sensor, a pH sensor, and a dissolved oxygen sensor are mounted in the culture tank 1. The culture tank 1 is arranged on a bracket structure, and the bottom plate of the bracket structure is provided with casters. The sealed cavities 12 are strip-shaped, and the sealed cavities 12 are uniformly distributed on the plate-shaped light source 8.
The foregoing describes the embodiments of the present utility model in detail, but the description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modification, equivalent replacement, improvement, etc. made within the scope of the present utility model should be included in the protection scope of the present utility model.
Claims (7)
1. The cell line culture and amplification device is characterized by comprising a culture tank (1), an input pipe (2), an output pipe (3), a bottom support frame (4), a circular ring support frame (5), a suspension pipe (6), a circular ring pipe (7), a plate-shaped light source (8), a port (9) and a light-transmitting pipe (10); the platy light source (8) comprises a support panel (11), a light-transmitting sealed cavity (12) is arranged on the support panel (11), an illuminating lamp (13) is arranged in the sealed cavity (12), a wire connector (14) is connected with the illuminating lamp (13), and the wire connector (14) sequentially penetrates through the annular tube (7) and the suspension tube (6) and is connected with a power supply; the input tube (2) and the output tube (3) are respectively located the top and the bottom of cultivate jar (1), bottom sprag frame (4) are fixed in cultivate jar (1), ring support frame (5) are connected on bottom sprag frame (4), fixedly connected with suspends pipe (6) in midair in cultivate jar (1), suspend pipe (6) and run through cultivate jar (1) top, ring form pipe (7) are connected in suspend pipe (6) bottom in midair, platy light source (8) fixed connection is provided with port (9) in cultivate jar (1) bottom between ring support frame (5) and ring form pipe (7), fixedly connected with light-transmitting pipe (10) in port (9) department, light-transmitting pipe (10) are located cultivate jar (1), light-transmitting pipe (10) top is sealed, install the light source in light-transmitting pipe (10).
2. A cell line culture expansion device according to claim 1, characterized in that a light tube is mounted in the light-transmitting tube (10), the line of which tube passes out through the port (9) and is connected to a power supply.
3. The cell line culture and amplification device according to claim 1, wherein an air supply pipeline is connected to the bottom end of the culture tank (1).
4. The cell line culture and amplification device according to claim 1, wherein a feed tube and an observation window are respectively connected to the top of the culture tank (1).
5. The cell line culture and amplification device according to claim 1, wherein a temperature sensor, a pH sensor and a dissolved oxygen sensor are installed in the culture tank (1).
6. A cell line culture expansion device according to claim 1, characterized in that the culture tank (1) is mounted on a support structure, on the floor of which the casters are mounted.
7. A cell line culture expansion device according to claim 1, wherein the sealed cavities (12) are in the form of strips, the sealed cavities (12) being evenly distributed over the plate-like light source (8).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321287565.3U CN219772144U (en) | 2023-05-25 | 2023-05-25 | Cell line culture and amplification device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321287565.3U CN219772144U (en) | 2023-05-25 | 2023-05-25 | Cell line culture and amplification device |
Publications (1)
Publication Number | Publication Date |
---|---|
CN219772144U true CN219772144U (en) | 2023-09-29 |
Family
ID=88132135
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202321287565.3U Active CN219772144U (en) | 2023-05-25 | 2023-05-25 | Cell line culture and amplification device |
Country Status (1)
Country | Link |
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CN (1) | CN219772144U (en) |
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2023
- 2023-05-25 CN CN202321287565.3U patent/CN219772144U/en active Active
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