CN219730953U - Stem cell culture equipment - Google Patents
Stem cell culture equipment Download PDFInfo
- Publication number
- CN219730953U CN219730953U CN202320085576.7U CN202320085576U CN219730953U CN 219730953 U CN219730953 U CN 219730953U CN 202320085576 U CN202320085576 U CN 202320085576U CN 219730953 U CN219730953 U CN 219730953U
- Authority
- CN
- China
- Prior art keywords
- dish
- culture
- stem cell
- cover
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 43
- 238000004113 cell culture Methods 0.000 title claims abstract description 29
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 26
- 229910052742 iron Inorganic materials 0.000 claims description 13
- 239000003822 epoxy resin Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229920000647 polyepoxide Polymers 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 239000006059 cover glass Substances 0.000 abstract description 31
- 210000004027 cell Anatomy 0.000 description 7
- 238000012258 culturing Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000013115 immunohistochemical detection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000003014 totipotent stem cell Anatomy 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a stem cell culture device, which belongs to the technical field of culture devices, and particularly relates to a stem cell culture device, comprising a culture dish and a dish cover, wherein a culture tank is arranged at the bottom of an inner cavity of the culture dish, the dish cover is arranged at the top of the culture dish, a pressing rod is arranged at the bottom of the dish cover, a cover glass matched with the culture tank is arranged at the bottom of the pressing rod, and the cover glass is embedded and installed in the culture tank.
Description
Technical Field
The utility model relates to the technical field of culture equipment, in particular to stem cell culture equipment.
Background
Stem cells are a type of pluripotent cells with self-replication ability, which can differentiate into a variety of functional cells under certain conditions, and are classified into embryonic stem cells and adult stem cells according to the developmental stage in which the stem cells are located, and three types according to the developmental potential of the stem cells: totipotent stem cells, pluripotent stem cells and monopotent stem cells, stem cells are an insufficiently differentiated, yet immature cell, and have the potential function of regenerating various tissues, organs and human bodies, and are called universal cells in the medical community.
In the prior art, in the stem cell culture process, cells grow on a cover glass, then the cover glass with the cells is taken out for subsequent detection such as immunohistochemical detection, when the cover glass is used, forceps are required to be operated to clamp the cover glass, the cover glass is easy to break in the clamping process, and the operation is inconvenient.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the utility model and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the utility model and in the title of the utility model, which may not be used to limit the scope of the utility model.
The present utility model has been made in view of the above and/or problems occurring in the prior art culture apparatuses.
Therefore, the utility model aims to provide the stem cell culture equipment which can facilitate the taking of the cover glass in the stem cell culture process, can detach the cover glass without operating forceps, prevents the cover glass from being broken in the clamping process and is convenient to operate.
In order to solve the technical problems, according to one aspect of the present utility model, the following technical solutions are provided:
the utility model provides a stem cell culture equipment, includes culture dish and dish lid, the bottom of culture dish inner chamber is provided with the culture tank, the dish lid sets up the top at the culture dish, the bottom of dish lid is provided with the depression bar, the bottom of depression bar is provided with the coverslip with culture tank matched with, the coverslip embedding is installed in the culture tank, be provided with the recess on the coverslip, the one end demountable installation of depression bar is in the recess, the bottom of dish lid is provided with magnet, the lateral wall of culture dish is provided with the iron sheet, culture dish and dish lid pass through magnet and iron sheet inter attraction and are connected.
As a preferred embodiment of the stem cell culture apparatus of the present utility model, wherein: the bottom of culture dish is provided with the slot, be provided with the inserted bar in the slot, the one end of inserted bar is provided with vacuum chuck.
As a preferred embodiment of the stem cell culture apparatus of the present utility model, wherein: the lateral wall of dish lid and culture dish all is provided with the rubber circle, the rubber circle all cup joints the lateral wall at dish lid and culture dish.
As a preferred embodiment of the stem cell culture apparatus of the present utility model, wherein: the number of the culture tanks is multiple, and the number of the cover slips is the same as that of the culture tanks.
As a preferred embodiment of the stem cell culture apparatus of the present utility model, wherein: the culture dish and the dish cover are made of transparent materials.
As a preferred embodiment of the stem cell culture apparatus of the present utility model, wherein: the side walls of the culture dish and the dish cover are provided with wear-resistant layers, and the wear-resistant layers are made of epoxy resin materials.
Compared with the prior art: according to the utility model, 1, stem cells are cultured through the culture tanks in the culture dish, the culture tanks are multiple, the culture tanks are convenient for culturing various stem cells, the culture space is saved, meanwhile, the culture results are convenient to observe in comparison during stem cell experiments, the culture tanks are covered on the tops of the culture dishes, the culture tanks and the dish covers are in adsorption connection through the magnets, the limit connection of the culture tanks and the dish covers is convenient, meanwhile, the press rod drives the cover glass to be embedded and installed in the culture tanks, the cover glass is convenient to cover in the culture tanks, when the cover glass is detached, the cover glass is convenient to detach through the magnets and the iron sheets, the cover glass is convenient to operate, the press rod and the cover glass are convenient to detach through the grooves, and the cover glass is convenient to replace.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following detailed description of the embodiments of the present utility model will be given with reference to the accompanying drawings, which are to be understood as merely some embodiments of the present utility model, and from which other drawings can be obtained by those skilled in the art without inventive faculty. Wherein:
FIG. 1 is a schematic diagram of a stem cell culture apparatus according to the present utility model;
FIG. 2 is an exploded view showing the structure of a stem cell culture apparatus according to the present utility model;
FIG. 3 is a sectional view showing the structure of a stem cell culture apparatus according to the present utility model;
FIG. 4 is a schematic diagram of a vacuum chuck of a stem cell culture apparatus according to the present utility model;
FIG. 5 is a schematic diagram of a slot of a stem cell culture apparatus according to the present utility model.
In the figure: 100 culture dishes, 110 culture tanks, 200 dish covers, 210 compression bars, 220 cover slips, 221 grooves, 230 magnets, 240 iron sheets, 300 slots, 310 inserting bars, 320 vacuum chucks and 400 rubber rings.
Detailed Description
In order that the above objects, features and advantages of the utility model will be readily understood, a more particular description of the utility model will be rendered by reference to the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model, but the present utility model may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present utility model is not limited to the specific embodiments disclosed below.
Next, the present utility model will be described in detail with reference to the drawings, wherein the sectional view of the device structure is not partially enlarged to general scale for the convenience of description, and the drawings are only examples, which should not limit the scope of the present utility model. In addition, the three-dimensional dimensions of length, width and depth should be included in actual fabrication.
For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, embodiments of the present utility model will be described in further detail below with reference to the accompanying drawings.
The utility model provides stem cell culture equipment, please refer to fig. 1-5, including culture dish 100 and dish cover 200, culture tank 110 is arranged at the bottom of the inner cavity of culture dish 100, dish cover 200 is arranged at the top of culture dish 100, pressure bar 210 is arranged at the bottom of dish cover 200, cover glass 220 matched with culture tank 110 is arranged at the bottom of pressure bar 210, cover glass 220 is embedded and installed in culture tank 110, recess 221 is arranged on cover glass 220, one end of pressure bar 210 is detachably installed in recess 221, magnet 230 is arranged at the bottom of dish cover 200, iron plate 240 is arranged on the side wall of culture dish 100, dish 100 and dish cover 200 are mutually attracted and connected with iron plate 240, in particular, dry cell culture is carried out through culture tank 110 in culture dish 100, the number of culture tank 110 is a plurality of, the convenience is brought to the culture of various stem cells, the space is saved, the convenience is brought to observe in the dry cell experiment, the convenience is covered at the top of culture tank 100 through dish cover 200, the convenience is connected with iron plate 240 through magnet 230 and iron plate 240 adsorption, thereby the convenience is brought to the dish cover glass 100 and the convenience is connected with iron plate 220, and the convenience is brought to the cover glass 220 is moved to the recess 220 and the convenience is moved upwards, the convenience is brought to the detachment of cover glass 220, and the convenience is brought to the detachment of cover glass 220 is carried out in the recess and the recess is only in connection with the recess 220, and the convenience is easy to detach operation is carried out.
The lateral wall of dish lid 200 and culture dish 100 all is provided with rubber circle 400, and rubber circle 400 all cup joints the lateral wall at dish lid 200 and culture dish 100, specifically, is convenient for protect dish lid 200 and culture dish 100's corner through rubber circle 400, and rubber circle 400 cup joints the top at dish lid 200 and the bottom of culture dish 100, has improved the protection effect of device.
The number of the culture tanks 110 is a plurality, and the number of the cover glass 220 is the same as that of the culture tanks 110, specifically, the culture of a plurality of groups of stem cells is facilitated, and the culture efficiency is improved.
The culture dish 100 and the dish cover 200 are made of transparent materials, and are particularly convenient for observing the culture process of stem cells and convenient for observation.
The side walls of the culture dish 100 and the dish cover 200 are provided with wear-resistant layers, the wear-resistant layers are made of epoxy resin materials, and specifically, the surfaces of the culture dish 100 and the dish cover 200 are more wear-resistant through the wear-resistant layers, so that scratches are prevented.
Referring to fig. 1 to 5, a stem cell culture apparatus according to the present embodiment is specifically used as follows: the culture tank 110 in the culture dish 100 is used for stem cell culture, the number of the culture tanks 110 is multiple, the culture tank is convenient for culturing multiple stem cells, the culture space is saved, meanwhile, when stem cell experiments are conducted, contrast observation is conducted, the culture result is convenient to observe, the operation dish cover 200 covers the top of the culture dish 100, the magnet 230 is used for being adsorbed and connected with the iron sheet 240, thereby being convenient for limit connection of the culture dish 100 and the dish cover 200, the dish cover 200 is connected with the top of the culture dish 100, the pressure bar 210 drives the cover glass 220 to be embedded and installed in the culture tank 110, the cover glass 220 is convenient to cover in the culture tank 110, the cover glass 220 is used for sealing the culture tank 110, and meanwhile, the stem cells are fixed, when the cover glass 220 is dismounted, only the operation dish cover 200 is required to be moved upwards, the magnet 230 is separated from the iron sheet 240, the cover glass 220 can be dismounted, the operation is convenient, the pressure bar 210 and the cover glass 220 are convenient to dismount through the groove 221, and replacement are convenient.
Fig. 4 to 5 are schematic structural views of a second embodiment of a stem cell culturing apparatus according to the present utility model, and referring to fig. 4 to 5, unlike the above embodiment, a socket 300 is provided at the bottom of a culture dish 100, a socket 310 is provided in the socket 300, a vacuum chuck 320 is provided at one end of the socket 310, and specifically, the socket 300 is cooperatively connected with the socket 310 to facilitate the installation or removal of the vacuum chuck 320, and the vacuum chuck 320 is adsorbed on a plane to facilitate the fixation of the culture dish, thereby facilitating the operation.
Although the utility model has been described hereinabove with reference to embodiments, various modifications thereof may be made and equivalents may be substituted for elements thereof without departing from the scope of the utility model. In particular, the features of the disclosed embodiments may be combined with each other in any manner as long as there is no structural conflict, and the exhaustive description of these combinations is not given in this specification merely for the sake of omitting the descriptions and saving resources. Therefore, it is intended that the utility model not be limited to the particular embodiment disclosed, but that the utility model will include all embodiments falling within the scope of the appended claims.
Claims (6)
1. A stem cell culture apparatus, characterized in that: including culture dish (100) and dish lid (200), the bottom of culture dish (100) inner chamber is provided with culture tank (110), dish lid (200) set up the top at culture dish (100), the bottom of dish lid (200) is provided with depression bar (210), the bottom of depression bar (210) is provided with and cultivates groove (110) matched with coverslip (220), coverslip (220) embedding is installed in culture tank (110), be provided with recess (221) on coverslip (220), the one end demountable installation of depression bar (210) is in recess (221), the bottom of dish lid (200) is provided with magnet (230), the lateral wall of culture dish (100) is provided with iron sheet (240), culture dish (100) and dish lid (200) are connected with iron sheet (240) inter attraction through magnet (230).
2. A stem cell culture apparatus according to claim 1, wherein: the bottom of culture dish (100) is provided with slot (300), be provided with inserted bar (310) in slot (300), the one end of inserted bar (310) is provided with vacuum chuck (320).
3. A stem cell culture apparatus according to claim 1, wherein: the side walls of the dish cover (200) and the culture dish (100) are respectively provided with a rubber ring (400), and the rubber rings (400) are respectively sleeved on the side walls of the dish cover (200) and the culture dish (100).
4. A stem cell culture apparatus according to claim 1, wherein: the number of the culture tanks (110) is a plurality, and the number of the cover slips (220) is the same as that of the culture tanks (110).
5. A stem cell culture apparatus according to claim 1, wherein: the culture dish (100) and the dish cover (200) are made of transparent materials.
6. A stem cell culture apparatus according to claim 1, wherein: the side walls of the culture dish (100) and the dish cover (200) are respectively provided with a wear-resistant layer, and the wear-resistant layers are made of epoxy resin materials.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202320085576.7U CN219730953U (en) | 2023-01-30 | 2023-01-30 | Stem cell culture equipment |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202320085576.7U CN219730953U (en) | 2023-01-30 | 2023-01-30 | Stem cell culture equipment |
Publications (1)
Publication Number | Publication Date |
---|---|
CN219730953U true CN219730953U (en) | 2023-09-22 |
Family
ID=88057122
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202320085576.7U Active CN219730953U (en) | 2023-01-30 | 2023-01-30 | Stem cell culture equipment |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN219730953U (en) |
-
2023
- 2023-01-30 CN CN202320085576.7U patent/CN219730953U/en active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2007265147B2 (en) | Method of culturing mesenchymal stem cells | |
JP2007167002A (en) | Cassette for cell culture, tool for attaching and detaching cassette for cell culture and cell culture apparatus | |
TW201510223A (en) | Method for separating living cell and constructing cell bank by means of tissue homogenate method | |
EP3321351B1 (en) | Cell-holding container and cell culture method using same | |
KR20160007659A (en) | Cell release method | |
CN219730953U (en) | Stem cell culture equipment | |
CN110564681A (en) | Isolated culture and nerve directional differentiation method of deciduous tooth pulp stem cells | |
CN1928063A (en) | High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus | |
CN107475110A (en) | A kind of highly effective pretreatment apparatus and its cultural method for being used to cultivate human amnion mesenchymal stem cell | |
CN107475111B (en) | Pretreatment device for culturing human umbilical cord mesenchymal stem cells and related application thereof | |
Kim et al. | Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells | |
CN221028474U (en) | Cell gene culture plate | |
CN2655245Y (en) | Closed biological sample tank easy-to-detach and clean | |
CN108728411B (en) | Isolated culture method of adipose-derived stem cells | |
CN213012906U (en) | Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells | |
CN209468437U (en) | A kind of Culture Mycoplasma device | |
CN215757397U (en) | High-flux cell culture device easy for cell to be detached from wall | |
RU2345781C2 (en) | Method of production of skin cell culture | |
CN201162013Y (en) | Large-scale sifting motion cultivation plate | |
CN220733916U (en) | Plant tissue culture dish | |
CN112813020B (en) | Human embryonic stem cells with specific HLA match | |
CN212640447U (en) | Detachable filamentous fungus culture apparatus | |
CN220099059U (en) | Molecular biology culture dish | |
JP2017046592A (en) | Cell culture container and cell subculture system as well as cell subculture method | |
CN203530338U (en) | Culture dish for observing living cells through microscope |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant |