CN2655245Y - Closed biological sample tank easy-to-detach and clean - Google Patents

Closed biological sample tank easy-to-detach and clean Download PDF

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Publication number
CN2655245Y
CN2655245Y CN 200320110116 CN200320110116U CN2655245Y CN 2655245 Y CN2655245 Y CN 2655245Y CN 200320110116 CN200320110116 CN 200320110116 CN 200320110116 U CN200320110116 U CN 200320110116U CN 2655245 Y CN2655245 Y CN 2655245Y
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CN
China
Prior art keywords
hole
cover glass
base
briquetting
chassis
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Expired - Fee Related
Application number
CN 200320110116
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Chinese (zh)
Inventor
王浩威
李银妹
楼立人
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University of Science and Technology of China USTC
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University of Science and Technology of China USTC
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Priority to CN 200320110116 priority Critical patent/CN2655245Y/en
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Publication of CN2655245Y publication Critical patent/CN2655245Y/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses a sealing biological sample pool which is easy for equipping, detaching and cleaning, characterized in that a middle convex section of a flat shaped pressing block 1 is a platform of a cover glass size with the middle made a via hole 9, a chassis 2 is a flat block with a hole 10 on the middle, the size of the hole 10 makes the cover glass 5 be able to be put in, the bottom portion of the hole 10 is maintained with step shaped edges to hold the cover glass 5 on the step from falling down, the depth of the hole 10 can make the chassis 2 orderly put the cover glass 5, a pad ring 3 and another cover glass 6 to form a cavity 4, then the edges beside the convex portion of the pressing block 1 and edges on the chassis 2 without hollowed portion are equipped with a gap below 1 mm, and the corresponding position on the pressing block 1 and the chassis are respectively embedded with a magnet 11 and a blind hole 7 and 8 of steel magnetic materials. The magnet forces are made use of to tightly appealing and closing the sample pool, the inner and the outer surface are equipped with channels and holes, which is quite smooth and is comparatively easy to be cleaned and sealed, the equipment and detachment are simple and convenient, no instrument is needed, which can avoid pollution brought from instruments.

Description

A kind of biological sample pond that is easy to install and remove the cleaning sealing
Technical field:
The utility model belongs to the Bioexperiment container technical field, is specifically related to the sample cell that Bioexperiment is used.
Background technology:
In biology, chemistry and material experiment, often need to make some sample cells.Adopt sometimes and will have the middle hole that cuts out a circle or other shape of certain thickness silicone rubber plate, cover cavity of formation at the hole upper and lower surface with slide, the sample that need seal just is placed among this cavity, again with slide and silicon rubber pad gummed or force together.Existing sample cell generally is that the fixation by screw thread or screw seals the mode that pad and slide compress.Because screw thread or screw all exist thread groove and hole, hide dirt easily, be difficult for cleaning and sterilizing; Use screw also convenient inadequately in operation, rotary screw is difficult more when especially wearing the gloves operation on aseptic platform; In addition, the instrument that is used for tightening up a screw also may become the source of pollution.
Technology contents:
The utility model provides the biological sample pond of a kind of easy mounting or dismounting, cleaning, sealing, to overcome the above-mentioned defective of existing sample cell.
This being easy to installed and removed the biological sample pond of cleaning sealing, comprises briquetting 1, base 2 and packing ring 3; It is characterized in that described briquetting 1 is the flat of big chain-wales of cover glass for the central protuberance cross section, the platform center scrapes out through hole 9; Base 2 is middle porose 10 flat, the size in this hole 10 can be put into cover glass 5, outstanding step-like edge is left in the bottom in hole 10 can make 5 of cover glasses can not fall on step, the degree of depth in hole 10 can make that putting into cover glass 5, packing ring 3 and an other cover glass 6 in the base 2 successively forms after the cavitys 4, and the marginal existence that edge beyond briquetting 1 bossing and base 2 do not have cut-out is less than 1 mm clearance; Briquetting 1 and base 2 are provided with the blind hole 7 and 8 that at least one group of opposite position is embedded with magnet 11 and ferrimagnet 12 respectively.
The comparable employed cover glass of the external dimensions of packing ring 3 is smaller, and the size of middle cavity 4 can determine according to the size of testing sample, but is not more than the size of cover glass; Can guarantee mutually reliable adhesive between its relative magnet 11 and the ferrimagnet 12 after the size of the cut-out in the middle of the base 2 should make briquetting 1 be placed on the base 2, generally should make the person's of relative magnet and ferrimagnet distance be no more than the 2-3 millimeter.
Compare with the sample cell that uses in the existing Bioexperiment, the utility model sample cell utilizes the magnetic force of magnetic material to make mounted sample cell become as a whole and provides enough magnetic attracting forces to make combining closely of slide and silicone rubber gasket; Cancelled screw thread and screw, the sample cell surface does not have complicated groove and hole, and the sample cell surfaces externally and internally is very smooth, therefore the sample cell ratio is easier to clean, installation and removal are very simple and convenient, do not need tool using, thereby can avoid the pollution that brought by instrument.
Description of drawings:
Fig. 1 is the utility model sample pool structure synoptic diagram.
Fig. 2 is the side view of a kind of concrete sample cell briquetting embodiment;
Fig. 3 is the vertical view of embodiment sample cell briquetting;
Fig. 4 is the side view of embodiment sample cell base;
Fig. 5 is the vertical view of embodiment sample cell base.
Embodiment:
Illustrate the embodiment in the utility model biological sample pond below in conjunction with accompanying drawing.
Embodiment 1:
Present embodiment biological sample pond adopts teflon to be processed into circular briquetting 1 and the base 2 of diameter 44mm respectively; What use is the standard cap slide of diameter as 32mm.Briquetting 1 gross thickness is 8mm, scrapes out the through hole 9 that diameter is 26mm in the middle of the platform of the thick 4mm of intermediate projections, diameter 32mm, platform; Base 2 gross thickness are 6mm, and the centre hollows out into through hole 10, the first half diameter 32mm that hollows out, dark 5mm, and the latter half that hollows out is the inwardly outstanding thick edge of 1mm, diameter 26mm.Briquetting 1 is drilled with the aperture 7 of 3 diameter 3mm, dark 3mm, and the position of this aperture 7 can take to be evenly distributed on the circumference of briquetting 1 and base 2 central diameter 37.5mm.Base 2 with the briquetting opposite position on be drilled with same diameter, the aperture 8 of dark 4mm.The magnetic material SmCo magnet steel 11 of the high-Curie-point that size is complementary with aperture and the aperture that iron block 12 is compressed into briquetting 1 and base 2 respectively fill up with 703 silica gel then.The silicone rubber plate that 1mm is thick makes external diameter 32mm, the circular packing ring 3 of internal diameter 26mm.
When above-mentioned biological sample pond is used for the laseropuncture transgenic experiments, must earlier briquetting 1, base 2 and silicone rubber gasket 3 be placed in the high-pressure sterilizing pot and sterilizes.Make the briquetting 1 and the base 2 of flat so adopt heat-resisting nonmetallic materials teflon in the present embodiment; Use nonmetallic materials silicone rubber plate heat-resisting and that have elasticity and a sealing property to make packing ring 3.
Base is placed on the aseptic platform, lies in the base 2 with a slice cover glass.Drip a little aqua sterilisa at the cover plate edge of 2 li of bases again, the silicone rubber gasket 3 after the sterilization is lain on the cover glass.Then preparation being carried out genetically modified blade of laser and plasmid solution is placed in the middle of the silicone rubber gasket 3.Drip a little aqua sterilisa at silicone rubber gasket 3 edges, cover on silicone rubber gasket 3 with other a slice cover glass sample is sealed.At last briquetting 1 is pressed, rotation briquetting 1, make in the briquetting 1 the relative also attraction of magnet steel with iron block in the base 2 together.The sample cell that installs can be placed directly in just putting or inverted microscope on observe or laseropuncture.Puncture is put back into sample cell on the aseptic platform after finishing.Open after carrying out disinfection with the alcohol outer surface, sample transfer is finished whole experiment to nutrient culture media.
The utility model utilizes the magnetic force of magnetic material to make mounted sample cell become as a whole and provides enough pressure to guarantee combining closely of slide and silicone rubber gasket; Owing to cancelled screw thread and screw, there are not complicated groove and hole, the sample cell surfaces externally and internally is very smooth, therefore cleans very easy.The installation and removal of the utility model sample cell can be finished with hand easily, do not need instrument, the pollution of having avoided instrument to bring.
Sample cell adopts heat-resisting nonmetallic materials teflon to make the briquetting 1 and the base 2 of flat in the present embodiment, use nonmetallic materials silicone rubber plate heat-resisting and that have elasticity and a sealing property to make packing ring 3, adopt the magnetic material SmCo magnet steel and the iron block of high-Curie-point to utilize the biological sample pond of magnetic with briquetting 1, base 2 and the easy sealing of packing ring 3 adhesives formation, can integral body carry out autoclave sterilization and can not demagnetize, can satisfy the needs of autoclave sterilization.

Claims (1)

1, a kind of being easy to installed and removed the biological sample pond of cleaning sealing, comprises briquetting (1), base (2) and packing ring (3); It is characterized in that described briquetting (1) is the flat of big chain-wales of cover glass for the central protuberance cross section, the platform center scrapes out through hole (9); Base (2) is middle porose (10) flat, the size in this hole (10) can be put into cover glass (5), outstanding step-like edge is left in the bottom in hole (10) can make cover glass (5) frame can not fall on step, the degree of depth in hole (10) can make that putting into cover glass (5), packing ring (3) and an other cover glass (6) in the base (2) successively forms cavity (4) afterwards, and the marginal existence that edge beyond briquetting (1) bossing and base (2) do not have cut-out is less than (1) mm clearance; Briquetting (1) and base (2) are provided with blind hole (7) and (8) that at least one group of opposite position is embedded with magnet (11) and ferrimagnet (12) respectively.
CN 200320110116 2003-10-10 2003-10-10 Closed biological sample tank easy-to-detach and clean Expired - Fee Related CN2655245Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200320110116 CN2655245Y (en) 2003-10-10 2003-10-10 Closed biological sample tank easy-to-detach and clean

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200320110116 CN2655245Y (en) 2003-10-10 2003-10-10 Closed biological sample tank easy-to-detach and clean

Publications (1)

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CN2655245Y true CN2655245Y (en) 2004-11-10

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102436267A (en) * 2011-11-04 2012-05-02 中国科学院长春光学精密机械与物理研究所 Apparatus for realizing microscale controllable property of liquid sample in sample cell in high vacuum environment and control method thereof
CN104267766A (en) * 2014-10-13 2015-01-07 中国科学技术大学 Real-time temperature-control micro sample room
CN104266973A (en) * 2014-10-13 2015-01-07 中国科学技术大学 Permanent liquid-state microsphere sample chamber
CN105807005A (en) * 2016-04-26 2016-07-27 温州大学 Liquid sample cell and manufacturing method thereof
CN106226235A (en) * 2016-08-15 2016-12-14 华中科技大学 A kind of sample cell for super-resolution location imaging system
CN106434273A (en) * 2015-08-06 2017-02-22 三鼎生物科技股份有限公司 Fixing device

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102436267A (en) * 2011-11-04 2012-05-02 中国科学院长春光学精密机械与物理研究所 Apparatus for realizing microscale controllable property of liquid sample in sample cell in high vacuum environment and control method thereof
CN102436267B (en) * 2011-11-04 2013-08-21 中国科学院长春光学精密机械与物理研究所 Apparatus for realizing microscale controllable property of liquid sample in sample cell in high vacuum environment
CN104267766A (en) * 2014-10-13 2015-01-07 中国科学技术大学 Real-time temperature-control micro sample room
CN104266973A (en) * 2014-10-13 2015-01-07 中国科学技术大学 Permanent liquid-state microsphere sample chamber
CN106434273A (en) * 2015-08-06 2017-02-22 三鼎生物科技股份有限公司 Fixing device
CN106434273B (en) * 2015-08-06 2018-12-11 三鼎生物科技股份有限公司 Fixing device
CN105807005A (en) * 2016-04-26 2016-07-27 温州大学 Liquid sample cell and manufacturing method thereof
CN105807005B (en) * 2016-04-26 2018-01-30 温州大学 A kind of liquid sample groove and preparation method thereof
CN106226235A (en) * 2016-08-15 2016-12-14 华中科技大学 A kind of sample cell for super-resolution location imaging system
CN106226235B (en) * 2016-08-15 2018-12-14 华中科技大学 A kind of sample cell for super-resolution positioning imaging system

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