CN215757397U - High-flux cell culture device easy for cell to be detached from wall - Google Patents
High-flux cell culture device easy for cell to be detached from wall Download PDFInfo
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- CN215757397U CN215757397U CN202121380500.4U CN202121380500U CN215757397U CN 215757397 U CN215757397 U CN 215757397U CN 202121380500 U CN202121380500 U CN 202121380500U CN 215757397 U CN215757397 U CN 215757397U
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Abstract
The utility model provides a high-flux cell culture device which is easy for cell wall removal. High flux cell culture ware that easily cell detached wall includes base, incubator, wall-separating mechanism, feed mechanism and collection mechanism, the upper surface of base is through a plurality of support column fixedly connected with supporting seats, incubator fixed connection is in the upper surface of supporting seat, be provided with the culture medium in the incubator, the last surface mounting of incubator has assorted sealing door, the fixed intercommunication in a side of incubator has the breather pipe, and installs assorted control valve on the breather pipe, the upper surface connection of supporting seat has the wall-separating mechanism that two symmetries set up. The high-flux cell culture device easy for cell wall removal provided by the utility model can enable the culture medium to generate tremble in the cell wall removal process, so that cells can be prevented from being adhered to the inner wall in the cell wall removal process, the cell wall removal efficiency is improved, and the cell wall removal is more thorough.
Description
Technical Field
The utility model relates to the field of household daily necessities, in particular to a high-flux cell culture device easy for cell de-walling.
Background
In recent decades, due to the rapid development of the biological product industry, the traditional method of obtaining biological products from animal tissues by biochemical technology has been far from meeting the market demand, and the expression of specific proteins, monoclonal antibodies, interferons and virus vaccine products by in vitro large-scale culture of animal cells has become the most common technology at present.
The Chinese utility model provides a high flux cell culture ware (notice number: CN212051466U) of easy cell wall removal, this device is in the use, through letting in sterile air to assist the cell to carry out the wall removal, but, it still has certain defect, and the in-process of removing the wall, the cell of cultivateing easily adheres on the inner wall, not only influences the efficiency that the cell removed the wall, also makes the cell wall removal incomplete easily.
Therefore, there is a need to provide a new high-throughput cell culture device which is easy to remove the cell wall to solve the above technical problems.
SUMMERY OF THE UTILITY MODEL
In order to solve the above technical problems, the present invention provides a high throughput cell culture device which can improve the efficiency of cell detachment and is easy to detach cells.
The utility model provides a high-flux cell culture device easy for cell wall removal, which comprises: the culture apparatus comprises a base, an incubator, a wall separating mechanism, a feeding mechanism and a collecting mechanism, wherein the upper surface of the base is fixedly connected with a supporting seat through a plurality of supporting columns, the incubator is fixedly connected with the upper surface of the supporting seat, a culture medium is arranged in the incubator, the upper surface of the incubator is provided with a matched sealing door, one side surface of the incubator is fixedly communicated with a vent pipe, a matched control valve is arranged on the vent pipe, the upper surface of the supporting seat is connected with two wall separating mechanisms which are symmetrically arranged, the wall separating mechanism comprises a motor, a rotating shaft, a rotating block and rubber strips, the upper surface of the supporting seat is provided with the motor, the output end of the motor is fixedly connected with the rotating shaft through a coupler, the upper end of the rotating shaft is fixedly connected with the rotating block, the side surface of the rotating block is fixedly connected with two rubber strips which are symmetrically arranged, and the feeding mechanism is connected with the upper surface of the base, and the feeding mechanism is communicated with the side surface of the incubator, the collecting mechanism is connected to the upper surface of the base, and the collecting mechanism is communicated with the other side surface of the incubator.
Preferably, feed mechanism includes culture solution case, support column two and leads to the liquid pipe, the upper surface of base is through two fixedly connected with culture solution cases of a plurality of support columns, and the last fixed surface intercommunication of culture solution case has the feed liquor pipe, threaded connection has the assorted lid on the feed liquor pipe, and the lower surface of culture solution case leads to the side fixed intercommunication of liquid pipe and incubator, and leads to and installs assorted control valve on the liquid pipe.
Preferably, the collecting mechanism comprises a collecting box and a collecting pipe, the upper surface of the base is fixedly connected with the collecting box, the upper surface of the collecting box is fixedly communicated with the side surface of the culture box through the collecting pipe, a liquid through hole corresponding to the collecting pipe is formed in one side surface of the culture medium, a discharging pipe is fixedly communicated with the side surface of the collecting box, and a matched control valve is installed on the discharging pipe.
Preferably, both sides face of incubator all fixedly connected with protection piece.
Preferably, the interior lower surface of incubator sets up two mounting grooves one that the symmetry set up, and the piece one is inhaled to assorted magnetism even to mounting groove one internal fixation, the mounting groove two that two symmetries set up is seted up to the lower surface of culture medium, and the piece two is inhaled to fixed connection and magnetism that the piece one assorted magnetism is inhaled to mounting groove two internal fixation is connected with.
Furthermore, two symmetrically arranged grooves are formed in the inner wall of the culture medium.
Compared with the related art, the high-flux cell culture device easy for cell wall removal provided by the utility model has the following beneficial effects:
the utility model provides a high-flux cell culture device easy for cell wall removal, which can cause a culture medium to generate tremble in the cell wall removal process, thereby preventing cells from being adhered to the inner wall in the cell wall removal process, improving the cell wall removal efficiency and ensuring that the cell wall removal is more complete.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a partial cross-sectional structural schematic of the present invention;
FIG. 3 is a schematic view showing the inside structure of the incubator of the present invention;
FIG. 4 is a schematic view of the structure of a medium in the present invention;
reference numbers in the figures: 1-a base; 2-supporting a column I; 3-a breather pipe; 4-an incubator; 5-liquid pipe; 6-culture solution box; 7-a collection tube; 8-a support seat; 9-an electric motor; 10-turning blocks; 11-rubber strips; 12-a guard block; 13-a sealing door; 14-support column two; 15-a collection box; 16-culture medium; 17-magnetic attraction block one; 18-liquid through hole; 19-magnetic block two; 20. a rotating shaft.
Detailed Description
The utility model is further described with reference to the following figures and embodiments.
Please refer to fig. 1, fig. 2, fig. 3 and fig. 4 in combination, wherein fig. 1 is a schematic structural diagram of the present invention; FIG. 2 is a partial cross-sectional structural schematic of the present invention; FIG. 3 is a schematic view showing the inside structure of the incubator of the present invention; FIG. 4 is a schematic view of the structure of a medium in the present invention. The method comprises the following steps: base 1, incubator 4, the mechanism of taking off the wall, feed mechanism and collection mechanism.
In the specific implementation process, as shown in fig. 1, fig. 2 and fig. 3, the upper surface of the base 1 is fixedly connected with a supporting seat 8 through a plurality of supporting columns one 2, the incubator 4 is fixedly connected with the upper surface of the supporting seat 8, a culture medium 16 is arranged in the incubator 4, the upper surface of the incubator 4 is provided with a matched sealing door 13 (an observation window is arranged on the sealing door 13 and is not marked in the figure), a side surface of the incubator 4 is fixedly communicated with a vent pipe 3, the vent pipe 3 is provided with a matched control valve, the upper surface of the supporting seat 8 is connected with two symmetrically arranged wall-separating mechanisms, each wall-separating mechanism comprises a motor 9, a rotating shaft 20, a rotating block 10 and a rubber strip 11, the upper surface of the supporting seat 8 is provided with a motor 9, the output end of the motor 9 is fixedly connected with the rotating shaft 20 through a coupler, and the upper end of the rotating block 10 is fixedly connected with the rotating shaft 20, two symmetrically arranged rubber strips 11 are fixedly connected to the side surface of the rotating block 10.
It is to be noted that; when using, in the culture medium 16 with cell culture and incubator 4, during the declivity, let in culture medium 16 by breather pipe 3 with sterile air, utilize sterile air to assist the cell to carry out the declivity, the declivity in-process, starting motor 9, the rotation of motor 9 drives the rotation of pivot 20, the rotation of pivot 20 drives the rotation of commentaries on classics piece 10, the rotation of commentaries on classics piece 10 drives two rubber strips 11 rotatoryly, beat incubator 4, make at the declivity in-process, culture medium 16 takes place the tremor, thereby can avoid cell declivity in-process cell adhesion at the inner wall, thereby not only improved the efficiency that the cell declivated, also it is more thorough that the cell declivity.
Referring to fig. 1 and 2, feed mechanism connects in the upper surface of base 1, and feed mechanism and incubator 4's side intercommunication, feed mechanism includes culture solution case 6, two 14 and logical liquid pipe 5 of support column, base 1's upper surface is through two 14 fixedly connected with culture solution cases 6 of a plurality of support columns, and culture solution case 6's last fixed surface intercommunication has the feed liquor pipe, threaded connection has the assorted lid on the feed liquor pipe, and culture solution case 6's lower surface is through the fixed intercommunication in side of leading to liquid pipe 5 and incubator 4, and installs the assorted control valve on leading to liquid pipe 5.
It is to be noted that; when the culture is carried out, the control valve of the liquid through pipe 5 is opened, so that the culture solution of the culture solution box 6 can be introduced into the culture medium 16 in the culture box 4 through the liquid through pipe 5, the culture solution can be fed in a sealed mode, and pollution is avoided when the culture solution is fed in.
Referring to fig. 1 and 2, the collecting mechanism is connected to the upper surface of the base 1 and is communicated with the other side surface of the incubator 4, the collecting mechanism comprises a collecting box 15 and a collecting pipe 7, the collecting box 15 is fixedly connected to the upper surface of the base 1, the upper surface of the collecting box 15 is fixedly communicated with the side surface of the incubator 4 through the collecting pipe 7, a liquid through hole 18 corresponding to the collecting pipe 7 is formed in one side surface of the culture medium 16, a discharging pipe is fixedly communicated with the side surface of the collecting box 15, and a matched control valve is installed on the discharging pipe.
It is to be noted that; when the culture is carried out, the control valve on the collecting pipe 7 is opened, so that the culture solution in the culture medium 16 can be discharged from the solution through hole 18 and then discharged into the collecting box 15 by the collecting pipe 7 for collection, the discharge can be sealed, and the pollution generated during the discharge can be avoided.
Referring to fig. 1, both sides of the incubator 4 are fixedly connected with a protection block 12, and in the process of detaching the wall, the rubber strip 11 is rotated to flap the protection block 12, so that the rubber strip 11 is prevented from directly flap the incubator 4, and a protection effect is achieved.
Refer to fig. 3 and 4 for it is shown that two mounting groove one that the symmetry set up is seted up to the interior lower surface of incubator 4, and the piece 17 is inhaled to assorted magnetism even to mounting groove one internal fixation, two mounting groove two that the symmetry set up are seted up to the lower surface of culture medium 16, and two internal fixedly connected with of mounting groove inhale piece two 19 with magnetism of inhaling a 17 assorted magnetism, inhale piece two 19 through magnetism and inhale piece 17 and magnetism the power of inhaling of piece two 19 and install culture medium 16 fixedly, make the dismantlement simple to operate of culture medium 16 swift.
Referring to FIG. 1, the inner wall of the culture medium 16 is formed with two symmetrically disposed grooves (not shown), so that the culture medium 16 can be lifted up through the grooves and the culture medium 16 can be drawn out of the incubator 4.
The working principle provided by the utility model is as follows: when the culture medium is used, sterile air is introduced into the culture medium 16 in the cell culture and culture box 4 through the vent pipe 3 during wall removal, the sterile air is used for assisting cells to be subjected to wall removal, the motor 9 is started during the wall removal process, the rotation of the motor 9 drives the rotation of the rotating shaft 20, the rotation of the rotating shaft 20 drives the rotating block 10 to rotate, the rotating block 10 drives the two rubber strips 11 to rotate and beat the culture box 4, so that the culture medium 16 is shaken during the wall removal process, cells can be prevented from being adhered to the inner wall during the wall removal process, the wall removal efficiency of the cells is improved, the cells can be removed more thoroughly, when the culture is carried out, the culture solution in the culture solution box 6 can be introduced into the culture medium 16 in the culture box 4 through the liquid introducing pipe 5 through opening the control valve of the liquid introducing pipe 5, sealed feeding can be realized, and pollution during feeding can be avoided, when culturing, the culture medium 16 can be discharged from the liquid through hole 18 by opening the control valve on the collection tube 7, and then discharged from the collection tube 7 into the collection box 15 for collection, so that the discharge can be sealed, and the pollution caused by the discharge can be avoided.
The circuits and controls involved in the present invention are prior art and will not be described in detail herein.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (6)
1. A high throughput cell culture apparatus that is susceptible to cell de-walling, comprising:
the device comprises a base (1), wherein the upper surface of the base (1) is fixedly connected with a supporting seat (8) through a plurality of supporting columns I (2);
the culture box (4) is fixedly connected to the upper surface of the supporting seat (8), a culture medium (16) is arranged in the culture box (4), a matched sealing door (13) is arranged on the upper surface of the culture box (4), a vent pipe (3) is fixedly communicated with one side surface of the culture box (4), and a matched control valve is arranged on the vent pipe (3);
the wall-removing mechanism is characterized in that the upper surface of the supporting seat (8) is connected with two symmetrically-arranged wall-removing mechanisms, each wall-removing mechanism comprises a motor (9), a rotating shaft (20), a rotating block (10) and rubber strips (11), the upper surface of the supporting seat (8) is provided with the motor (9), the output end of the motor (9) is fixedly connected with the rotating shaft (20) through a coupler, the upper end of the rotating shaft (20) is fixedly connected with the rotating block (10), and the side surfaces of the rotating block (10) are fixedly connected with the two symmetrically-arranged rubber strips (11);
the feeding mechanism is connected to the upper surface of the base (1) and is communicated with the side surface of the incubator (4);
the collecting mechanism is connected to the upper surface of the base (1) and communicated with the other side surface of the incubator (4).
2. The high-flux cell culture device easy for cell wall detachment according to claim 1, wherein the feeding mechanism comprises a culture solution box (6), two support columns (14) and a liquid passing pipe (5), the upper surface of the base (1) is fixedly connected with the culture solution box (6) through the two support columns (14), the upper surface of the culture solution box (6) is fixedly communicated with a liquid inlet pipe, the liquid inlet pipe is connected with a matched cover body in a threaded manner, the lower surface of the culture solution box (6) is fixedly communicated with the side surface of the culture box (4) through the liquid passing pipe (5), and the liquid passing pipe (5) is provided with a matched control valve.
3. The high-flux cell culture device easy for cell wall detachment according to claim 1, wherein the collection mechanism comprises a collection box (15) and a collection pipe (7), the collection box (15) is fixedly connected to the upper surface of the base (1), the upper surface of the collection box (15) is fixedly communicated with the side surface of the culture box (4) through the collection pipe (7), a liquid through hole (18) corresponding to the collection pipe (7) is formed in one side surface of the culture medium (16), a discharge pipe is fixedly communicated with the side surface of the collection box (15), and a matched control valve is mounted on the discharge pipe.
4. The high-throughput cell culture device easy for cell detachment according to claim 1, wherein both sides of the culture box (4) are fixedly connected with protective blocks (12).
5. The high-flux cell culture device easy for cell wall detachment according to claim 1, wherein the inner lower surface of the incubator (4) is provided with two first mounting grooves which are symmetrically arranged, the first mounting grooves are fixedly connected with the first magnetic attraction blocks (17) which are matched with each other, the lower surface of the culture medium (16) is provided with two second mounting grooves which are symmetrically arranged, and the second mounting grooves are fixedly connected with the second magnetic attraction blocks (19) which are matched with the first magnetic attraction blocks (17) which are matched with each other.
6. The high-throughput cell culture device facilitating cell detachment according to claim 1, wherein the inner wall of the culture medium (16) is formed with two symmetrically arranged grooves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121380500.4U CN215757397U (en) | 2021-06-21 | 2021-06-21 | High-flux cell culture device easy for cell to be detached from wall |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121380500.4U CN215757397U (en) | 2021-06-21 | 2021-06-21 | High-flux cell culture device easy for cell to be detached from wall |
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| Publication Number | Publication Date |
|---|---|
| CN215757397U true CN215757397U (en) | 2022-02-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121380500.4U Active CN215757397U (en) | 2021-06-21 | 2021-06-21 | High-flux cell culture device easy for cell to be detached from wall |
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| Country | Link |
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| CN (1) | CN215757397U (en) |
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2021
- 2021-06-21 CN CN202121380500.4U patent/CN215757397U/en active Active
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