CN219701721U - Biological reaction mixing device - Google Patents

Abstract

The utility model provides a biological reaction mixing device, which comprises a storage unit and a mixing unit, wherein the storage unit and the mixing unit are respectively connected with a fluid connection unit in a sealing way; the gas conveying runner is communicated with an external gas source; the mixing generating device is contacted or connected with one side of the mixing unit far away from the fluid connection unit, and the mixing generating device provides external force for mixing the reaction substances for the mixing unit. Through respectively with storage unit and mixing unit sealing connection with fluid connection unit, and make mixing unit and fluid connection unit flexonics, can place the reactant in advance in the storage unit, through outside air supply and valve way control system control gas circuit or liquid way's intercommunication or disconnection to in the reactant input mixing unit that places in advance in the appointed storage unit, the external force that the reuse mixing generating device provided, the fluid that makes mixing unit inside produces the high-speed vortex motion that takes the turbulent flow effect as leading, helps increasing the mixing effect.

Description

Biological reaction mixing device

Technical Field

The utility model relates to the technical field of biological detection equipment research and development, in particular to a biological reaction mixing device.

Background

Currently, commercial nucleic acid extraction and purification methods mainly include a spin column method and a magnetic bead method. In the magnetic bead method, magnetic microspheres with surfaces coated with silicon materials or other materials capable of binding nucleic acids are used as solid phase carriers, and DNA and RNA are separated from a sample under the action of chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an externally applied magnetic field. The method is simple to operate, is easy to realize automatic and high-flux operation, and has the defects of higher cost, easiness in influence on extraction efficiency and larger change of magnetic bead extraction effects of different samples, different manufacturers and different batches.

When the magnetic bead method is used for extracting nucleic acid, the lysate, the cleaning solution and the eluent are fully mixed with the magnetic bead mixed solution of the sample to ensure better extraction effect. The traditional nucleic acid extraction instrument is mixed by impacting liquid through the mutual movement of a magnetic rod sleeve and a deep pore plate, and detection pollution is often caused by the fact that samples are splashed inside the instrument in the impacting process due to different proportions of various original factory reagents. In patent CN104971638A, the stirring device invented by the manufacturer uses the characteristic that the gear drives the stirring rod sleeve to stir, so that the maximum treatment flux in unit area can be achieved, and the problem of cross contamination can be effectively reduced.

When nucleic acid is extracted by the magnetic bead method, besides the biological reagent is fully mixed, the magnetic beads with DNA cell information are transferred to various reagent positions, and the magnetic attraction and heating processes are carried out. In the patent CN214088455U, in the large-scale nucleic acid extraction automation workstation instrument system, a manner such as a rotary shielding plate is used to directly shield the upper part of the deep-hole plate, for example, the inside of a part of the model nucleic acid extraction automation workstation manufactured by Qiagen in germany is protected from pollution by adopting the manner, and the mechanical structure is directly used to physically shield the deep-hole plate, so that the large-scale nucleic acid extraction automation workstation instrument system has a very good pollution prevention effect, but at the same time, the shielding structure such as the rotary shielding plate type requires a larger working space and a more complex mechanical assembly to drive, so that the instrument miniaturization is not facilitated.

In the field of liquid mixing, in particular to the field of sample and reagent mixing subdivision in the in-vitro diagnosis industry, the mainstream mixing solutions existing at present comprise bubble mixing, in-plane swinging mixing, ultrasonic vibration mixing, eccentric mixing, magnetic bead mixing and the like. For this reason, there is a continuing need in the art to develop a high efficiency mixing device for nucleic acid extraction.

Disclosure of Invention

In view of the drawbacks of the prior art, an object of the present utility model is to provide a bioreactor mixing device.

The biological reaction mixing device comprises a storage unit, a mixing unit, a fluid connection unit and a mixing generation device, wherein the storage unit and the mixing unit are respectively and hermetically connected with the fluid connection unit, and the mixing unit is elastically connected with the fluid connection unit; the fluid connection unit comprises a gas conveying runner and a liquid conveying runner, any one of the storage units is respectively communicated with the gas conveying runner and the liquid conveying runner, and any one of the mixing units is respectively communicated with the gas conveying runner and the liquid conveying runner; the gas conveying runner is communicated with an external gas source, the mixing generating device is contacted or connected with one side of the mixing unit far away from the fluid connection unit, and the mixing generating device provides external force for mixing the reaction substances for the mixing unit.

Preferably, the storage unit includes a first extension tube, and the mixing unit includes a second extension tube; one end of the first extension tube is communicated with the liquid conveying runner, the other end of the first extension tube extends towards the inside of the storage unit, and a gap is formed between the first extension tube and the bottom of the storage unit; one end of the second extension tube is communicated with the liquid conveying runner, the other end of the second extension tube extends towards the inside of the mixing unit, and a gap is formed between the second extension tube and the bottom of the mixing unit.

Preferably, the fluid connection unit further comprises a pressure relief opening, the mixing unit is communicated with the pressure relief opening, and a plug is detachably connected to the pressure relief opening.

Preferably, the external gas source comprises a positive pressure gas source or a negative pressure gas source; the external air source comprises one or more of an air pump, a high-pressure air tank, a vacuum pump or a piston device.

Preferably, the fluid connection unit comprises a runner main board, the gas conveying runner and the liquid conveying runner are both integrated on the runner main board, the runner main board and the mixing unit are both made of hard non-deformable materials, and the runner main board and the mixing unit are connected in a sealing mode through flexible deformable materials.

Preferably, the fluid connection unit comprises a flow channel main plate, both the gas delivery flow channel and the liquid delivery flow channel are integrated on the flow channel main plate, the material of the flow channel main plate comprises a hard non-deformable material, the material of the mixing unit comprises a flexible deformable material, and the flow channel main plate and the mixing unit are connected in a sealing manner.

Preferably, the fluid connection unit includes a flow channel main plate, both the gas delivery flow channel and the liquid delivery flow channel are integrated on the flow channel main plate, both the flow channel main plate and the mixing unit are flexible deformable materials, and both the flow channel main plate and the mixing unit are molded at one time.

Preferably, the fluid connection unit comprises a substrate, a runner main board and a valve path switching board, and the substrate, the runner main board and the valve path switching board are sequentially connected in a stacked manner; the flow channel main board is provided with a gas conveying groove and a liquid conveying groove, the storage unit and the mixing unit are both connected to one side of the base plate, which is away from the valve path switching board, and the storage unit and the mixing unit are respectively communicated with the gas conveying groove and the liquid conveying groove; the valve path control system comprises a driving device, a cipher roller, a valve rod and a return spring, wherein the driving device drives the cipher roller to rotate, the valve rod is in contact with the outer surface of the cipher roller, and the return spring exerts force on the valve rod.

Preferably, the mixing generator comprises a contact unit, an eccentric unit and a driving unit, wherein the driving unit is in transmission connection with the eccentric unit, the eccentric unit is in transmission connection with the contact unit, and the contact unit is in contact with the mixing unit; the mixing external force provided by the mixing generator comprises one or more of mechanical vibration, ultrasonic vibration and in-plane swing mixing.

Preferably, the mixing unit is heated by one or more of thermal convection, infrared or thermal radiation; the magnetic device comprises a magnetic body, the magnetic body is arranged in the mixing unit, and the magnetic body comprises magnetism or demagnetization.

Compared with the prior art, the utility model has the following beneficial effects:

1. according to the utility model, the contact unit is contacted with the mixing unit, the driving unit drives the eccentric unit to rotate and drives the contact unit, so that fluid in the mixing unit generates high-speed vortex motion taking turbulence effect as a main factor, bubbles can be generated to be uniformly mixed while the high-speed vortex motion is performed, bubbles with different shapes and flow velocities are generated by changing the size of the airflow and the size of the flow channel, and the uniform mixing effect is effectively increased.

2. According to the utility model, the storage unit and the mixing unit are respectively connected with the fluid connection unit in a sealing way, and the mixing unit is flexibly connected with the fluid connection unit, so that the reactive substance can be placed in the storage unit in advance, and the connection or disconnection of the gas path or the liquid path is controlled by an external gas source and a valve path control system, so that the reactive substance placed in the designated storage unit in advance is input into the mixing unit, and then the external force provided by the mixing generation device is utilized, so that the fluid in the mixing unit generates high-speed vortex motion taking the turbulence effect as a dominant effect, thereby being beneficial to increasing the mixing effect.

3. According to the utility model, the fluid connection unit adopts the micro-fluidic chip, the mixing generation device is utilized to provide external force for mixing reactants to the mixing unit, after the reactants in the mixing unit are completely mixed, the mixed reactants are transferred to the micro-fluidic chip for subsequent operation, so that the mixing degree of the reactants is improved, and the mixing time of the reactants is saved.

4. The utility model enables the mixing unit to be combined with the magnetic device, the temperature control device and the like in a quick mode by arranging the mixing unit outside the fluid connection unit, and the functions required by more biological reactions are realized during the mixing operation.

5. The utility model controls the connection or disconnection of the gas path and the liquid path through the valve path control system, so that the mixing unit can ensure sealing when mixing, thereby being beneficial to reducing the phenomenon that liquid in the mixing unit can not splash out of the mixing unit when mixing uniformly, and further being beneficial to reducing instrument pollution.

6. According to the utility model, through full sealing of the hose, bubbles can be added for uniform mixing, and the height adjustment of the liquid vortex is realized by changing the rotating speed of the vortex.

Drawings

Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:

FIG. 1 is a schematic view showing the overall structure of a biological reaction mixing device according to a first embodiment of the present utility model;

FIG. 2 is a schematic view showing the overall structure of a fluid connection unit according to a first embodiment of the present utility model;

FIG. 3 is a schematic view showing the overall structure of a biological reaction mixing apparatus having an intermediate unit according to the present utility model;

FIG. 4 is a schematic view showing the overall structure of a biological reaction mixing device according to the present utility model;

FIG. 5 is an exploded view showing the overall structure of a fluid coupling unit in accordance with a preferred embodiment of the present utility model;

FIG. 6 is a schematic view showing the overall structure of a runner motherboard in accordance with a preferred embodiment of the present utility model;

fig. 7 is a schematic diagram showing the overall structure of a valve path control system according to a first preferred embodiment of the present utility model.

Reference numerals:

fluid connection unit 1 drive unit 43

Temperature control device 5 of gas conveying runner 101

Liquid conveying runner 102 magnetic device 6

Intermediate unit 7 of first extension tube 103

Piston device 8 of second extension tube 104

Pressure relief port 105 piston head 81

Base plate 11 piston connecting rod 82

Linear driving motor 83 of piston cylinder buckle 112

Flow passage main board 12 valve path control system 9

Gas delivery slot 120 stepper motor 91

Coupling 92 of liquid conveying trough 121

Valve way switching plate 13 cipher roller 93

Storage unit 2 miniature ball bearing 94

Mixing unit 3-bar securing block 95

Mixing and generating device 4 valve stem 96

Return spring 97 of contact unit 41

Eccentric unit 42

Detailed Description

The present utility model will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present utility model, but are not intended to limit the utility model in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present utility model.

Example 1

As shown in fig. 1, 2 and 3, the multifunctional biological reaction mixing device according to the present utility model includes a storage unit 2, a mixing unit 3, a fluid connection unit 1, a mixing generating device 4, a temperature control device 5 and a magnetic device 6. Both the storage unit 2 and the mixing unit 3 are respectively connected with the fluid connection unit 1 in a sealed manner, and the mixing unit 3 is elastically connected with the fluid connection unit 1.

The fluid connection unit 1 comprises a gas conveying flow channel 101 and a liquid conveying flow channel 102, any storage unit 2 is respectively communicated with the gas conveying flow channel 101 and the liquid conveying flow channel 102, any mixing unit 3 is respectively communicated with the gas conveying flow channel 101 and the liquid conveying flow channel 102, the gas conveying flow channel 101 is communicated with an external gas source, the mixing generation device 4 is contacted with or connected with one side of the mixing unit 3 away from the fluid connection unit 1, and the mixing generation device 4 provides external force for mixing reaction substances for the mixing unit 3. The temperature control device 5 heats the mixing unit 3. The magnetic means 6 are actually magnetic or demagnetized.

Specifically, the storage unit 2 includes a first extension tube 103, and the mixing unit 3 includes a second extension tube 104. One end of the first extension tube 103 is communicated with the liquid conveying runner 102, the other end of the first extension tube 103 extends towards the inside of the storage unit 2, and a gap is formed between the first extension tube 103 and the bottom of the storage unit 2. One end of the second extension tube 104 is communicated with the liquid conveying runner 102, the other end of the second extension tube 104 extends towards the inside of the mixing unit 3, and a gap is formed between the second extension tube 104 and the bottom of the mixing unit 3.

The fluid connection unit 1 of the present utility model comprises a microfluidic chip on which both the liquid delivery flow channel 102 and the gas delivery flow channel 101 are integrated. Both the storage unit 2 and the mixing unit 3 may be test tools such as test tubes, beakers, plastic bottles, etc., and it is preferred that both the storage unit 2 and the mixing unit 3 are transparent containers in the present utility model. The reactive substance may be stored in advance in any of the storage units 2, or may be stored in advance in some of the storage units 2, and the other storage unit 2 does not contain any reactive substance. The reactive substance in the storage unit 2 may be a liquid, a gum, a solid powder, solid particles or a suspension of solid particles and a liquid, etc. So long as the reactant species can pass through the liquid delivery flow channel 102.

Further, the fluid connection unit 1 further comprises a pressure relief opening 105, the mixing unit 3 is communicated with the pressure relief opening 105, and a plug is detachably connected to the pressure relief opening 105. Opening the pressure relief 105 allows the excess gas in the mixing unit 3 to be vented, thereby ensuring that the gas pressure in the mixing unit 3 is the same as the external gas pressure.

The mixing generating device 4 provides power, and the external mixing force provided by the mixing generating device 4 comprises one or more of mechanical vibration, ultrasonic vibration and in-plane swinging and mixing. Specifically, the mixing generating device 4 includes a contact unit 41, an eccentric unit 42, and a driving unit 43, the driving unit 43 is in transmission connection with the eccentric unit 42, the eccentric unit 42 is in transmission connection with the contact unit 41, and the contact unit 41 is in contact with the mixing unit 3. The centrifugal force generated by the contact of the contact unit 41 and the mixing unit 3 causes the liquid inside the mixing unit 3 to flow outwards, thereby forming vortex and lifting flow, and finally achieving the purpose of uniformly mixing the liquid in the mixing unit 3.

The magnetic device 6 comprises a magnetic body which is arranged in the mixing unit 3, and the magnetic body comprises magnetism feeding or demagnetization, and the magnetic bead mixed liquor product in the mixing unit 3 is magnetically attracted. The temperature control means 5 heats the mixing unit 3 by one or more of thermal convection, infrared or thermal radiation. The biological mixing is accelerated by heating the magnetic bead mixture in the mixing unit 3.

It should be further noted that, in order to increase the force exerted by the mixing generating device 4 on the mixing unit 3, the connection between the mixing unit 3 and the fluid connection unit 1 is a flexible sealing connection, and the mixing unit 3 may be elastically deformed or radially moved relative to the fluid connection unit 1 under the action of an external force.

One possible implementation is: the fluid connection unit 1 comprises a runner main board 12, both a gas conveying runner 101 and a liquid conveying runner 102 are integrated on the runner main board 12, both the runner main board 12 and the mixing unit 3 are made of hard non-deformable materials, and the runner main board 12 and the mixing unit 3 are connected in a sealing manner through flexible deformable materials. In particular, an intermediate unit 7 of flexible deformable material may be employed to flexibly seal the mixing unit 3 to the flow channel main plate 12.

Another possible implementation is: the fluid connection unit 1 comprises a flow channel main plate 12, both the gas delivery flow channel 101 and the liquid delivery flow channel 102 are integrated on the flow channel main plate 12, the material of the flow channel main plate 12 comprises a hard non-deformable material, the material of the mixing unit 3 comprises a flexible deformable material, and the flow channel main plate 12 and the mixing unit 3 are connected in a sealing manner.

Yet another possible implementation is: the fluid connection unit 1 includes a flow path main plate 12, both the gas delivery flow path 101 and the liquid delivery flow path 102 are integrated on the flow path main plate 12, both the flow path main plate 12 and the mixing unit 3 are flexible deformable materials, and both the flow path main plate 12 and the mixing unit 3 are molded in one step.

Example two

Based on the first embodiment, as shown in fig. 4, the present utility model is illustrated by taking three storage units 2 and one mixing unit 3 provided on the fluid connection unit 1 as an example of a multifunctional biological reaction mixing apparatus according to the present utility model.

The three storage units 2 are respectively communicated with the liquid conveying runner 102 through a first extension tube 103, and the mixing unit 3 is communicated with the liquid conveying runner 102 through a second extension tube 104. The three storage units 2 are all communicated with the gas conveying flow channel 101 through gas source through holes. The external air source provides air source power, the external air source comprises a positive pressure air source or a negative pressure air source, and the external air source comprises one or a combination of a plurality of air pumps, high-pressure air tanks, vacuum pumps or piston devices 8. The external air source is communicated with the air source driving interface of the air conveying flow channel 101 through a sealing connecting piece, and a positive pressure source is provided in the air conveying flow channel 101.

The gas source pressure input by the external gas source respectively enters the three storage units 2, and the reaction substances stored in the three storage units 2 can enter the liquid conveying flow channel 102 through the corresponding first extension tube 103 and then flow into the mixing unit 3 through the liquid conveying flow channel 102.

The system also comprises a valve path control system 9, wherein the valve path control system 9 controls the communication between the gas conveying flow channel 101 and any one of the storage unit 2 or the mixing unit 3, and the valve path control system 9 controls the communication between the liquid conveying flow channel 102 and any one of the storage unit 2 or the mixing unit 3. The valve control system 9 includes a gas path valve and a liquid path valve, the gas path valve controls the storage unit 2 to be connected to or disconnected from the gas delivery flow path 101, and the liquid path valve controls the storage unit 2 to be connected to or disconnected from the liquid delivery flow path 102. Through the cooperation of gas circuit valve and liquid way valve, can realize blocking simultaneously the gas circuit and the liquid way of a storage unit 2, at this moment, the air supply pressure of the air supply drive input of outside air supply gets into respectively in two other storage units 2, and the reaction material in two storage units 2 can get into liquid transport runner 102 through corresponding first extension pipe 103, later flows into in the mixing unit 3 through liquid transport runner 102, and the reaction material in the storage unit 2 of blocked gas circuit and liquid way is unchangeable. The transfer of the reactants in the designated storage unit 2 into the mixing unit 3 is achieved.

Preferred embodiment one

As shown in fig. 4, 5 and 6, according to the first and/or second embodiments, the fluid connection unit 1 according to the present utility model includes a substrate 11, a flow channel main board 12 and a valve path switching board 13, where the substrate 11, the flow channel main board 12 and the valve path switching board 13 are sequentially stacked and connected. The base plate 11 is disposed downward on a side facing away from the valve path switching plate 13, and three storage units 2, one mixing unit 3, and one piston cartridge 112 are sequentially mounted below the base plate 11. The upper end of the piston cylinder button 112 is provided with a through hole which is communicated with the gas conveying flow channel 101 of the fluid connection unit 1.

The runner main board 12 is provided with a gas conveying groove 120 and a liquid conveying groove 121, the gas conveying groove 120 is composed of a main groove and a plurality of sub-grooves communicated with the main groove, the source of the main groove is communicated with a through hole at the upper end of the piston cylinder buckle 112, any sub-groove is provided with a through hole, and any sub-groove is communicated with one storage unit 2 through the through hole, so that a plurality of storage units 2 are respectively communicated with the gas conveying groove 120. The liquid conveying groove 121 is composed of a main groove and a plurality of sub grooves communicated with the main groove, a through hole is formed in any sub groove, and the through hole in the sub groove of the liquid conveying groove 121 is connected with the first extension pipe 103 or the second extension pipe 104, so that the plurality of storage units 2 and the mixing unit 3 are respectively communicated with the liquid conveying groove 121. The runner main board 12 further comprises a pressure relief opening 105 communicated with the mixing unit 3, and the pressure relief opening 105 is communicated with the mixing unit 3 through a pressure relief groove.

Both the storage unit 2 and the mixing unit 3 are connected to the side of the base plate 11 facing away from the valve path switching plate 13, and both the storage unit 2 and the mixing unit 3 are respectively communicated with the gas delivery flow passage 101 and the liquid delivery flow passage 102.

The valve path control system 9 is arranged on one side of the valve path switching plate 13 away from the substrate 11, and the valve path control system 9 comprises a gas path valve and a liquid path valve, wherein the gas path valve controls the connection or disconnection of any storage unit 2 and the gas conveying flow channel 101, and the liquid path valve controls the connection or disconnection of any storage unit 2 and the liquid conveying flow channel 102.

As shown in fig. 7, in particular, the present utility model provides one possible embodiment of the valve path control system 9: the valve path control system comprises a driving device, a cipher roller 93, a valve rod 96 and a return spring 97, wherein the driving device drives the cipher roller 93 to rotate, the valve rod 96 is in contact with the outer surface of the cipher roller 93, and the return spring 97 applies force to the valve rod 96. The driving device can adopt a stepping motor 91, the stepping motor 91 is in transmission connection with a code roller 93 through a coupler 92, and a groove is arranged on the outer surface of the code roller 93. The upper end of valve rod 96 is provided with pole fixed block 95, and the one end that pole fixed block 95 deviates from valve rod 96 is provided with miniature ball bearing 94, miniature ball bearing 94 and cipher roller 93 rolling contact. The force exerted by the return spring 97 on the valve stem 96 maintains the miniature ball bearing 94 in rolling contact with the combination roller 93.

In a normal state, each valve rod 96 applies a force to the valve path switching plate 13 and blocks the corresponding gas delivery flow passage 101 or liquid delivery flow passage 102. The stepping motor 91 drives the cipher roller 93 to rotate, when the miniature ball bearing 94 at the top end of the valve rod 96 moves into the groove, the valve rod 96 moves in the direction close to the surface of the cipher roller 93 under the action of the return spring 97, and the corresponding gas conveying flow channel 101 or liquid conveying flow channel 102 is conducted.

The external air source of the present utility model is preferably a piston assembly 8, the piston assembly 8 including a piston head 81, a piston connecting rod 82, and a linear drive motor 83.

Specifically, the storage unit 2 of the substrate 11 is filled with the corresponding biological reagent in advance, and the mixing unit 3 is filled with the magnetic biological sample. The valve path control system 9 can switch the valve paths by adopting a solenoid valve system through an electric control method, so that the opening or closing of the paths of each storage unit 2 is realized. The piston device 8 provides positive pressure to transfer the liquid in the storage unit 2 into the main groove of the liquid conveying groove 121 of the runner main board 12 through the first extension pipe 103, transfer into the mixing unit 3 through the groove dividing hole of the liquid conveying groove 121 and the second extension pipe 104, and discharge the air pressure in the mixing unit 3 to the atmosphere through the pressure relief groove and the pressure relief opening 105 on the runner main board 12, so that the air pressure in the mixing unit 3 is discharged to the atmosphere, and the transfer of the liquid path is completed.

In the same way, the valve control system 9 is used to control the communication or closing of the liquid channel and the gas channel of each storage unit 2, and the piston device 8 provides positive pressure, so that the liquid in the storage unit 2 can be transferred to the mixing unit 3. The solenoid valve electric control method is the most common method for switching the top valve path, and it should be understood that the valve path control system 9 of the present utility model is not limited to the specific embodiment described above.

More specifically, the mixing generating device 4 is disposed at a lower portion of the mixing unit 3, and the external force of the mixing of the reaction substances provided by the mixing generating device 4 includes one or more of mechanical vibration force, magnetic force, thermal convection, infrared ray, and thermal radiation. Under the action of the mixing generating device 4, the lower end part of the mixing unit 3 generates high-speed vortex motion, and then fluid in the mixing unit 3 is driven to generate high-speed vortex motion taking turbulence effect as dominant, so that the rapid and effective mixing effect of the fluid in the mixing unit 3 is realized. It is important to note that the mixing generator 4 of the present utility model may be designed in different forms, or may be designed to mix without the aid of a swirling motion. For example, mixing can also be performed by way of a back and forth reciprocating motion, provided that the motion can cause turbulence of the fluid inside the mixing member to achieve rapid and efficient mixing.

The mixing generating device 4 may not contact with the mixing unit 3, but contact with the runner main board 12 or the intermediate unit 7, so that the liquid in the mixing unit 3 generates turbulence to realize rapid and efficient mixing.

The magnetic means 6 may be a permanent magnet material, the up-and-down movement of which effects the up-and-down magnetization of the magnet. The magnetic induction device can also be arranged as an electromagnet, and the magnetization and demagnetization are realized through the control of an external power supply.

Further, the temperature control device 5 heats or cools the liquid inside the mixing unit 3. The temperature control device 5 can heat or cool the liquid inside the mixing unit 3 by means of heat conduction. At this time, the temperature control device 5 is connected by a movement mechanism, and when it is necessary to perform a heating or cooling action on the liquid inside the mixing unit 3, the temperature control device 5 contacts the mixing unit 3 under the driving of the movement mechanism. On the contrary, when the heating or cooling process is finished, the temperature control device 5 is driven by the motion mechanism to leave the mixing unit 3, so that a space is left, and the mixing unit 3 can elastically displace or deform. The temperature control device 5 may be fixed to the mixing unit 3, and may heat or cool the mixing unit 3 by a non-contact method such as heat convection or radiation.

In the description of the present utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

The foregoing describes specific embodiments of the present utility model. It is to be understood that the utility model is not limited to the particular embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without affecting the spirit of the utility model. The embodiments of the utility model and the features of the embodiments may be combined with each other arbitrarily without conflict.

Claims (10)

1. The biological reaction mixing device is characterized by comprising a storage unit (2), a mixing unit (3), a fluid connection unit (1) and a mixing generation device (4), wherein the storage unit (2) and the mixing unit (3) are respectively and hermetically connected with the fluid connection unit (1), and the mixing unit (3) is elastically connected with the fluid connection unit (1);

the fluid connection unit (1) comprises a gas conveying runner (101) and a liquid conveying runner (102), any one of the storage units (2) is respectively communicated with the gas conveying runner (101) and the liquid conveying runner (102), and any one of the mixing units (3) is respectively communicated with the gas conveying runner (101) and the liquid conveying runner (102);

the gas conveying flow channel (101) is communicated with an external gas source, the mixing generation device (4) is in contact with or connected with one side of the mixing unit (3) away from the fluid connection unit (1), and the mixing generation device (4) provides external force for mixing reaction substances for the mixing unit (3).

2. The biological reaction mixing device according to claim 1, wherein the storage unit (2) comprises a first extension tube (103), the mixing unit (3) comprising a second extension tube (104);

one end of the first extension tube (103) is communicated with the liquid conveying runner (102), the other end of the first extension tube (103) extends towards the inside of the storage unit (2), and a gap is formed between the first extension tube (103) and the bottom of the storage unit (2);

one end of the second extension tube (104) is communicated with the liquid conveying runner (102), the other end of the second extension tube (104) extends towards the inside of the mixing unit (3), and a gap is formed between the second extension tube (104) and the bottom of the mixing unit (3).

3. The biological reaction mixing device according to claim 1, wherein the fluid connection unit (1) further comprises a pressure relief port (105), the mixing unit (3) is communicated with the pressure relief port (105), and a plug is detachably connected to the pressure relief port (105).

4. The biological reaction mixing device of claim 1, wherein the external gas source comprises a positive pressure gas source or a negative pressure gas source;

the external air source comprises one or more of an air pump, a high pressure air tank, a vacuum pump or a piston device (8).

5. The biological reaction mixing device according to claim 1, characterized in that the fluid connection unit (1) comprises a flow channel main plate (12), that both the gas delivery flow channel (101) and the liquid delivery flow channel (102) are integrated on the flow channel main plate (12), that both the flow channel main plate (12) and the mixing unit (3) are of a rigid non-deformable material, and that the flow channel main plate (12) and the mixing unit (3) are sealingly connected by a flexible deformable material.

6. The biological reaction mixing device according to claim 1, characterized in that the fluid connection unit (1) comprises a flow channel main plate (12), that both the gas delivery flow channel (101) and the liquid delivery flow channel (102) are integrated on the flow channel main plate (12), that the material of the flow channel main plate (12) comprises a stiff non-deformable material, that the material of the mixing unit (3) comprises a flexible deformable material, and that the flow channel main plate (12) and the mixing unit (3) are sealingly connected.

7. The biological reaction mixing device according to claim 1, characterized in that the fluid connection unit (1) comprises a flow channel main plate (12), that both the gas delivery flow channel (101) and the liquid delivery flow channel (102) are integrated on the flow channel main plate (12), that both the flow channel main plate (12) and the mixing unit (3) are of flexible deformable material, and that both the flow channel main plate (12) and the mixing unit (3) are molded in one piece.

8. The biological reaction mixing device according to claim 1, wherein the fluid connection unit (1) comprises a substrate (11), a flow channel main board (12) and a valve path switching board (13), and the substrate (11), the flow channel main board (12) and the valve path switching board (13) are sequentially stacked and connected;

the flow channel main board (12) is provided with a gas conveying groove (120) and a liquid conveying groove (121), the storage unit (2) and the mixing unit (3) are both connected to one side of the base board (11) away from the valve path switching board (13), and the storage unit (2) and the mixing unit (3) are respectively communicated with the gas conveying groove (120) and the liquid conveying groove (121);

the valve path control system (9) is further included, the valve path control system (9) comprises a driving device, a cipher roller (93), a valve rod (96) and a return spring (97), the driving device drives the cipher roller (93) to rotate, the valve rod (96) is in contact with the outer surface of the cipher roller (93), and the return spring (97) exerts force on the valve rod (96).

9. The biological reaction mixing device according to claim 1, characterized in that the mixing generating device (4) comprises a contact unit (41), an eccentric unit (42) and a drive unit (43), the drive unit (43) being in driving connection with the eccentric unit (42), the eccentric unit (42) being in driving connection with the contact unit (41), the contact unit (41) being in contact with the mixing unit (3);

the mixing external force provided by the mixing generating device (4) comprises one or more of mechanical vibration, ultrasonic vibration and in-plane swinging and mixing.

10. The biological reaction mixing device according to claim 1, further comprising temperature control means (5) and magnetic means (6), said temperature control means (5) heating the mixing unit (3) by one or more of thermal convection, infrared or thermal radiation;

the magnetic means (6) comprise a magnetic body which is arranged in the mixing unit (3) and which comprises a magnetizing or demagnetizing.