CN219670501U - Detection module of fluorescent quantitative PCR instrument - Google Patents
Detection module of fluorescent quantitative PCR instrument Download PDFInfo
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- CN219670501U CN219670501U CN202320756162.2U CN202320756162U CN219670501U CN 219670501 U CN219670501 U CN 219670501U CN 202320756162 U CN202320756162 U CN 202320756162U CN 219670501 U CN219670501 U CN 219670501U
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- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 238000003753 real-time PCR Methods 0.000 title claims abstract description 15
- 239000013307 optical fiber Substances 0.000 claims abstract description 21
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 3
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- 108020004414 DNA Proteins 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 206010036618 Premenstrual syndrome Diseases 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The utility model discloses a detection module of a fluorescence quantitative PCR instrument, which comprises a driving device, a supporting component and a signal receiving device, wherein the signal receiving device is arranged at the bottom of the supporting component, and the driving device is arranged above the supporting component; the supporting component comprises a turntable and a turntable mounting plate, the turntable is rotatably arranged on the turntable mounting plate, the driving device is detachably arranged on the turntable mounting plate, and the driving device is used for driving the turntable to rotate; the signal receiving device comprises a receiving module and a transmitting module, wherein the receiving module and the transmitting module are arranged below the turntable, a plurality of first mounting holes are formed in the receiving module and the transmitting module, a plurality of second mounting holes are formed in the turntable mounting plate, the number and the positions of the first mounting holes and the second mounting holes are corresponding, and optical fibers are placed in the first mounting holes and the second mounting holes; the utility model aims to solve the problems of weak detection specificity and low analysis sensitivity of a PCR instrument detection module in the prior art.
Description
Technical Field
The utility model relates to the technical field of PCR instruments, in particular to a detection module of a fluorescent quantitative PCR instrument.
Background
In the field of molecular biology, the polymerase chain reaction technique (Polymerase Chain Reaction, abbreviated as PCR) is a commonly used experimental technique whose basic principle is similar to the natural replication process of DNA, and is a method for in vitro enzymatic promotion of the synthesis of specific DNA fragments, whose specificity depends on oligonucleotide primers complementary to both ends of a target sequence. The PCR is basically formed by three basic reaction steps of deformation, annealing and extension, has the advantages of strong specificity, high sensitivity, simple and convenient operation, time saving and the like, can be used for basic researches such as gene separation, cloning, nucleic acid sequence analysis and the like, and is more and more widely applied to diagnosis of clinical diseases. The instrument for realizing the real-time fluorescent quantitative PCR technology is called a real-time fluorescent quantitative PCR instrument, is essentially a temperature control device, and can accurately control the denaturation temperature, the renaturation temperature and the extension temperature. The basic principle is as follows: the light source lamp emits excitation light, the excitation light reaches the reaction tube with the sample through the optical component, and the sample emits fluorescence after being excited. The excited fluorescence then passes through the optical assembly to the detector. The filter is a component of the optical assembly that allows light of a specific wavelength to pass through, thereby achieving the purpose of distinguishing products. The fluorescence passing through the filter lens is captured by the detector after passing through the optical component, and the captured fluorescence is input into a computer for calculation and analysis after a series of photoelectric conversion. Common detectors for fluorescent PCR are cameras, PMTs (photomultiplier tubes), photodiodes, etc.
In a traditional fluorescence detection system, excitation light is emitted through a light source, the excitation light is excited after passing through an incident light filter, and the excited fluorescence passes through a detection light filter and then reaches a detector (such as a photodiode and a camera). However, whether a photodiode or a camera is used as a detector, a filter is used to obtain fluorescence of a desired wavelength. The multi-channel fluorescence detection needs a plurality of filters, and fluorescence with different wavelengths is selected or the filters are rotated through the filters; or various filters are mounted on an optical head, and scanned hole by the back and forth movement of the optical head. Both of these filtering modes require mechanical movement means, otherwise the required fluorescence is not obtained. The mechanical moving structure (device) is introduced, so that the instrument is complex and huge, and the cost is high; more importantly, the motion makes the whole instrument in a dynamic detection system, equipment faults are extremely easy to cause, and huge cost and burden are brought to subsequent maintenance. In addition, the optics of a conventional PCR instrument is typically located at the top of the instrument. The excitation light source projects the sample to be detected in the sample cell after passing through the incident filter, and the fluorescence excited by the sample to be detected is directly projected onto a photosensitive receiving tube or a photoelectric detector such as a CCD after being filtered by the detection light filter, so that the target of quantitative detection of the sample to be detected is realized. Excitation light irradiates the sample tube from the top of the sample base to excite fluorescence and realize detection, the method has particularly high requirements on the quality of the caps of the sample reaction tube and the reagent plate, has high transmittance, and is particularly easy to cause optical path confusion.
Disclosure of Invention
The utility model provides a detection module of a fluorescent quantitative PCR instrument, and aims to solve the problems of weak detection specificity and low analysis sensitivity of the detection module of the PCR instrument in the prior art.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the detection module of the fluorescent quantitative PCR instrument comprises a driving device, a supporting component and a signal receiving device, wherein the signal receiving device is arranged at the bottom of the supporting component, and the driving device is arranged above the supporting component;
the supporting component comprises a turntable and a turntable mounting plate, the turntable is rotatably arranged on the turntable mounting plate, the driving device is detachably arranged on the turntable mounting plate, and the driving device is used for driving the turntable to rotate;
the signal receiving device comprises a receiving module and a transmitting module, wherein the receiving module and the transmitting module are arranged below the turntable, a plurality of first mounting holes are formed in the receiving module and the transmitting module, a plurality of second mounting holes are formed in the turntable mounting plate, the number and the positions of the first mounting holes and the second mounting holes are corresponding, and optical fibers are placed in the first mounting holes and the second mounting holes; the transmitting module is connected with the transmitting end of the optical fiber, and the receiving module is connected with the receiving end of the optical fiber; the miniature cap-type slip ring is arranged below the turntable, the lines on the receiving module and the transmitting module are connected with the inner ring of the miniature cap-type slip ring, and the appearance of the miniature cap-type slip ring is detachably connected with the turntable.
Further, the driving device comprises a motor, and an output shaft of the motor penetrates through the turntable mounting plate and is detachably connected with the turntable.
Further, through holes are formed in four corners of the motor, threaded holes are formed in the turntable mounting plate, the number and positions of the threaded holes correspond to those of the through holes, bolts are arranged in the through holes, and the bolts are in threaded connection with the threaded holes.
Further, the output shaft of the motor is connected with the turntable through a coupling.
Further, the signal receiving device is provided with a plurality of groups, and the signal receiving devices of the groups are all arranged below the turntable.
Further, the turntable mounting plate is provided with digital scales.
Further, the bottom of the turntable is provided with a mounting frame, and an outer ring of the miniature cap-type slip ring is detachably connected with the mounting frame.
Further, the mounting frame comprises three upright posts arranged at the bottom of the turntable in an array manner, one ends of the three upright posts are connected with the bottom of the turntable, and the other ends of the three upright posts are detachably connected with the outer ring of the miniature cap-type slip ring.
Compared with the prior art, the utility model has the following beneficial effects:
the utility model mainly comprises a driving device, a supporting component and a signal receiving device, wherein the signal receiving device is arranged at the bottom of the supporting component, and the driving device is arranged above the supporting component; in the actual use process, the transmitting module is used for detecting the liquid to be detected through the optical fiber, the receiving module is used for receiving the optical fiber signal detected by the liquid to be detected, the first mounting hole corresponds to the second mounting hole in position, the optical fiber can be mounted on the first mounting hole, and the optical fiber can emit light beams through the second mounting hole; the detection of the liquid to be detected is completed through the simultaneous working of the transmitting module and the receiving module; the driving device can drive the turntable to rotate, the turntable rotates to enable the receiving module and the transmitting module below the turntable to rotate, at the moment, the optical fiber above the turntable mounting disc irradiates onto the other group of receiving module and the transmitting module, the liquid to be detected is detected again, the miniature cap slip ring is arranged to prevent the receiving module and the transmitting module from winding when rotating, the detecting device is used for enhancing the specificity of the liquid to be detected through detection for a plurality of times, and the detected sensitivity is higher.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present utility model.
FIG. 2 is a second schematic diagram of the structure of the present utility model.
In the figure, a 1-motor, a 2-coupler, a 3-turntable mounting plate, a 4-turntable, a 5-transmitting module, a 6-receiving module, a 7-miniature cap-type slip ring, an 8-first mounting hole, a 9-second mounting hole, a 10-digital scale and an 11-upright post.
Detailed Description
The present utility model is further described below in conjunction with embodiments, which are merely some, but not all embodiments of the present utility model. Based on the embodiments of the present utility model, other embodiments that may be used by those of ordinary skill in the art without making any inventive effort are within the scope of the present utility model.
Example 1
Referring to fig. 1 and 2, the present embodiment discloses a detection module of a fluorescent quantitative PCR apparatus, including a driving device, a supporting component, and a signal receiving device, wherein the signal receiving device is disposed at the bottom of the supporting component, and the driving device is disposed above the supporting component;
the support assembly comprises a turntable 4 and a turntable mounting plate 3, the turntable 4 is rotatably arranged on the turntable mounting plate 3, the driving device is detachably arranged on the turntable mounting plate 3, and the driving device is used for driving the turntable 4 to rotate;
the signal receiving device comprises a receiving module 6 and a transmitting module 5, wherein the receiving module 6 and the transmitting module 5 are arranged below the turntable 4, a plurality of first mounting holes 8 are formed in the receiving module 6 and the transmitting module 5, a plurality of second mounting holes 9 are formed in the turntable mounting plate 3, the number and positions of the first mounting holes 8 and the second mounting holes 9 are corresponding, and optical fibers are placed in the first mounting holes 8 and the second mounting holes 9; the transmitting module 5 is connected with the transmitting end of the optical fiber, and the receiving module 6 is connected with the receiving end of the optical fiber; the miniature cap-type slip ring 7 is arranged below the turntable 4, the lines on the receiving module 6 and the transmitting module 5 are connected with the inner ring of the miniature cap-type slip ring 7, and the appearance of the miniature cap-type slip ring 7 is detachably connected with the turntable 4;
the utility model mainly comprises a driving device, a supporting component and a signal receiving device, wherein the signal receiving device is arranged at the bottom of the supporting component, and the driving device is arranged above the supporting component; in the actual use process, the transmitting module 5 is used for detecting the liquid to be detected through the optical fiber, the receiving module 6 is used for receiving the optical fiber signal detected by the liquid to be detected, the first mounting hole 8 corresponds to the second mounting hole 9 in position, the purpose is that the optical fiber can be mounted on the first mounting hole 8, and the optical fiber can emit light beams through the second mounting hole 9; the detection of the liquid to be detected is completed through the simultaneous working of the transmitting module 5 and the receiving module 6; the driving device can drive the turntable 4 to rotate, the turntable 4 rotates to enable the receiving module 6 and the transmitting module 5 below the turntable 4 to rotate, at the moment, the optical fiber above the turntable mounting plate 3 irradiates onto the other group of receiving module 6 and the transmitting module 5, the liquid to be detected is detected again, the miniature cap slip ring 7 is arranged to prevent the receiving module 6 and the transmitting module 5 from winding when rotating, and the detection device is used for enhancing the specificity of the detection of the liquid to be detected through detection for a plurality of times, so that the detected sensitivity is higher.
Further, the driving device comprises a motor 1, and an output shaft of the motor 1 penetrates through a turntable mounting plate 3 and is detachably connected with a turntable 4; in practical use, the driving device is not limited to the motor 1, but in this embodiment, the driving device may be the motor 1, and the turntable 4 is driven to rotate after the motor 1 rotates.
Further, through holes are formed in four corners of the motor 1, threaded holes are formed in the turntable mounting plate 3, the number and positions of the threaded holes correspond to those of the through holes, bolts are arranged in the through holes and are in threaded connection with the threaded holes, in the practical use process, workers can fix the motor 1 on the turntable mounting plate 3 after the bolts are matched with the threaded holes, and therefore the motor 1 is convenient to install.
Further, the output shaft of the motor 1 is connected with the turntable 4 through a coupler 2, in the actual use process, a round hole is formed in the turntable mounting plate 3, the output shaft of the motor 1 passes through the coupler 2 and is connected with the turntable 4 through the round hole, and the coupler 2 is rotationally connected with the round hole.
Further, the signal receiving devices are provided with a plurality of groups, and the signal receiving devices of the groups are arranged below the turntable 4; in this embodiment, a receiving module 6 and a transmitting module 5 form a set of signal receiving devices, and the signal receiving devices have six sets, and the six sets of signal receiving devices are disposed at the bottom of the turntable 4.
Further, the turntable mounting plate 3 is provided with a digital scale 10, each first mounting hole 8 is provided with a corresponding digital scale 10, and the purpose of the digital scale 10 is to count the number of optical fibers conveniently.
Further, the bottom of the turntable 4 is provided with a mounting frame, and an outer ring of the mini-type slip ring 7 is detachably connected with the mounting frame.
Further, the mounting frame comprises three upright posts 11 arranged at the bottom of the turntable 4 in an array manner, one end of each upright post 11 is connected with the bottom of the turntable 4, and the other end of each upright post is detachably connected with the outer ring of the micro cap type slip ring 7.
In the description of the present utility model, it should be understood that the terms "coaxial," "bottom," "one end," "top," "middle," "another end," "upper," "one side," "top," "inner," "front," "center," "two ends," etc. indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
Furthermore, the terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated, whereby features defining "first," "second," "third," "fourth" may explicitly or implicitly include at least one such feature.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "configured," "connected," "secured," "screwed," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intermediaries, or in communication with each other or in interaction with each other, unless explicitly defined otherwise, the meaning of the terms described above in this application will be understood by those of ordinary skill in the art in view of the specific circumstances.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a detection module of fluorescence ration PCR appearance which characterized in that: the device comprises a driving device, a supporting component and a signal receiving device, wherein the signal receiving device is arranged at the bottom of the supporting component, and the driving device is arranged above the supporting component;
the support assembly comprises a turntable (4) and a turntable mounting plate (3), the turntable (4) is rotatably arranged on the turntable mounting plate (3), the driving device is detachably arranged on the turntable mounting plate (3), and the driving device is used for driving the turntable (4) to rotate;
the signal receiving device comprises a receiving module (6) and a transmitting module (5), wherein the receiving module (6) and the transmitting module (5) are arranged below the turntable (4), a plurality of first mounting holes (8) are formed in the receiving module (6) and the transmitting module (5), a plurality of second mounting holes (9) are formed in the turntable mounting plate (3), the number and positions of the first mounting holes (8) and the second mounting holes (9) are corresponding, and optical fibers are placed in the first mounting holes (8) and the second mounting holes (9); the transmitting module (5) is connected with the transmitting end of the optical fiber, and the receiving module (6) is connected with the receiving end of the optical fiber; the miniature cap type slip ring (7) is arranged below the rotary table (4), the lines on the receiving module (6) and the transmitting module (5) are connected with the inner ring of the miniature cap type slip ring (7), and the appearance of the miniature cap type slip ring (7) is detachably connected with the rotary table (4).
2. The detection module of a fluorescent quantitative PCR instrument according to claim 1, wherein: the driving device comprises a motor (1), and an output shaft of the motor (1) penetrates through the turntable mounting disc (3) and is detachably connected with the turntable (4).
3. The detection module of a fluorescent quantitative PCR instrument according to claim 2, wherein: through holes are formed in four corners of the motor (1), threaded holes are formed in the turntable mounting plate (3), the number and positions of the threaded holes correspond to those of the through holes, bolts are arranged in the through holes, and the bolts are in threaded connection with the threaded holes.
4. A detection module of a fluorescent quantitative PCR instrument according to claim 3, wherein: the output shaft of the motor (1) is connected with the rotary table (4) through a coupler (2).
5. The detection module of a fluorescent quantitative PCR instrument according to claim 1, wherein: the signal receiving devices are provided with a plurality of groups, and the signal receiving devices of the groups are all arranged below the turntable (4).
6. The detection module of a fluorescent quantitative PCR instrument according to claim 1, wherein: the turntable mounting plate (3) is provided with digital scales (10).
7. The detection module of a fluorescent quantitative PCR instrument according to claim 1, wherein: the bottom of the turntable (4) is provided with a mounting frame, and an outer ring of the miniature cap-type slip ring (7) is detachably connected with the mounting frame.
8. The detection module of claim 7, wherein: the mounting bracket includes three stand (11) of array setting in carousel (4) bottom, and three stand (11) one end are connected with carousel (4) bottom, and the other end is dismantled with the outer loop of miniature cap type sliding ring (7) and is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320756162.2U CN219670501U (en) | 2023-04-07 | 2023-04-07 | Detection module of fluorescent quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320756162.2U CN219670501U (en) | 2023-04-07 | 2023-04-07 | Detection module of fluorescent quantitative PCR instrument |
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| Publication Number | Publication Date |
|---|---|
| CN219670501U true CN219670501U (en) | 2023-09-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320756162.2U Active CN219670501U (en) | 2023-04-07 | 2023-04-07 | Detection module of fluorescent quantitative PCR instrument |
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| Country | Link |
|---|---|
| CN (1) | CN219670501U (en) |
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- 2023-04-07 CN CN202320756162.2U patent/CN219670501U/en active Active
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