CN206362706U - A kind of optical detection apparatus of real-time fluorescent PCR amplification instrument - Google Patents
A kind of optical detection apparatus of real-time fluorescent PCR amplification instrument Download PDFInfo
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- CN206362706U CN206362706U CN201720014415.3U CN201720014415U CN206362706U CN 206362706 U CN206362706 U CN 206362706U CN 201720014415 U CN201720014415 U CN 201720014415U CN 206362706 U CN206362706 U CN 206362706U
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- optical
- deflecting plate
- detection apparatus
- plate
- fluorescence
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Abstract
The utility model belongs to field of gene detection, more particularly to a kind of optical detection apparatus of real-time fluorescent PCR amplification instrument, and it includes the light emitting module, scan module and the detection module for receiving fluorescence for being used to send exciting light；Light emitting module includes the light source being disposed adjacent, aperture, first optical filter and speculum, scan module includes deflecting plate, controller, drive mechanism and object lens, object lens are placed in the surface of specimen mount, deflecting plate is placed in the top of object lens, drive mechanism is used to drive deflecting plate to rotate, controller is communicated to connect with drive mechanism, detection module includes photodetector, array of photoswitch and fibre bundle, fibre bundle includes multifiber, the fluorescence that optical fiber is used to send in sample in plate hole is transmitted to photodetector, array of photoswitch is connected with fibre bundle, for being controlled to the fluorescence transmission in optical fiber.The utility model employs the mode of the lateral fluorescent collecting of reaction tube, improves the sensitivity and the degree of accuracy of measurement.
Description
Technical field
The utility model belongs to field of gene detection, the optical detection dress of more particularly to a kind of real-time fluorescent PCR amplification instrument
Put.
Background technology
Detection of nucleic acids has sensitivity high compared with other detection the pathogenic microorganism examination methods, and specificity is good, and window phase is short, detection
The advantages of time is short.Current nucleic acid of pathogenic microorganism detection method mainly has polymerase chain reaction (Polymerase Chain
Reaction, PCR), rolling circle amplification and ring mediated isothermal amplification etc..Wherein, round pcr is the most universal, wherein and with
Real-time fluorescence PCR (Real-time PCR) technology is the most commonly used, and PCR is that one kind is designed according to DNA replication dna property in organism
External rapid amplifying specific dna sequence technology.PCR reaction systems it is main by nucleic acid primer, 4 kinds of dNTP, archaeal dna polymerase,
Template DNA and PCR reaction buffers system composition.Real-Time Fluorescent Quantitative PCR Technique refers to add fluorescence in PCR reaction systems
Group, monitors whole PCR processes, finally by standard curve to not in real time using the accumulation of fluorescence signal during DNA cloning
Know the method that template carries out quantitative analysis.Compared with common PCR, Real-Time Fluorescent Quantitative PCR Technique has high specificity, sensitive
Degree is high, reproducible, quantify the advantages of accurate, fast speed, totally-enclosed reaction, realizes PCR quantitative analysis.This technology tool
Have sensitivity high, the features such as specific good, but need real-time fluorescence PCR instrument costly, in particular by multiple light courcess,
The PCR instrument of multi-detector, photoswitch or complicated machinery sweep mechanism, such as multiple light courcess PCR instrument, it is provided solely for each sample
Vertical light source, complicated it is necessary to set corresponding 96 or 384 light sources for 96 or 384 conventional samples, dimension
Repair too high with use cost, limit application.
Utility model content
In order to overcome the defect of prior art, the purpose of this utility model is to provide a kind of for the real-time of genetic test
The optical detection apparatus of fluorescent PCR amplification instrument, the optical detection apparatus is simple in construction, easy to operate, it is easy to repair, can be quick
Quantitative analysis is carried out to gene in real time.
What the utility model was realized in：
Specifically, the utility model provides a kind of optical detection apparatus of real-time fluorescent PCR amplification instrument, including：For sending out
Go out the light emitting module, scan module and the detection module for receiving fluorescence of exciting light；
The light emitting module includes the light source, aperture, the first optical filter and the speculum that are disposed adjacent, and the exciting light is from light
Source is sent, and the scan module is incided after sequentially passing through aperture, the first optical filter and speculum；
The scan module includes deflecting plate, controller, drive mechanism and object lens, and the object lens are being placed in specimen mount just
Top, the deflecting plate is placed in the top of the object lens, and the drive mechanism is used to drive deflecting plate to rotate, the controller with
The drive mechanism communication connection, the exciting light incides the deflecting plate after speculum reflects；
The detection module includes photodetector, array of photoswitch and fibre bundle, and the fibre bundle includes many and institute
The one-to-one optical fiber of multiple plate holes in specimen mount is stated, the optical fiber is used for send sample reaction tube in the plate hole
Fluorescence is transmitted to the photodetector, what the array of photoswitch was connected with fibre bundle, for being transmitted to the fluorescence in optical fiber
It is controlled；
The controller is communicated to connect with the array of photoswitch.
Preferably, the drive mechanism includes stepper motor, and the controller is used to send instruction, stepping to stepper motor
Motor is used to drive deflecting plate to rotate by axle of the trunnion axis of deflecting plate according to the instruction.
Preferably, the deflecting plate is rotated around its trunnion axis in certain angle, the angle ranging from the normal of deflecting plate with
The angle angle of vertical direction, the angle ranging from 0-25 degree.
Preferably, the detection module also includes the second optical filter, and first optical filter and the second optical filter are can
Optical filter is adjusted, is respectively used to filter to exciting light and fluorescence.
Preferably, the multifiber includes fibre-optical splice, and the fibre-optical splice is used to optical fiber being fixed on sample bottom
In the plate hole of seat, the fibre-optical splice is fixed in the plate hole of specimen mount by helicitic texture.
Preferably, the fibre-optical splice be located at the plate hole in, make optical fiber relative with the side wall of sample reaction tube, gather from
The fluorescence that the side wall is sent.
Preferably, the deflecting plate is glass plate, plastics or resinous flat.
Preferably, the upper and lower surface of deflecting plate is coated with anti-reflection film.
Preferably, the light source is laser, xenon lamp, LED or Halogen lamp LED.
Compared with prior art, the utility model has the advantages that：
1. single unit system only needs to control the movement of one moving part of deflecting plate, you can realize that light beam is scanned, simple in construction, into
This is low, it is easy to safeguard；
2. it is that full plate can be achieved to excite only with a light source without setting independent light source one by one for reaction tube quantity,
Fluorescent PCR measurement is completed, therefore the versatility of device is stronger；
3. the mode of the lateral fluorescent collecting of reaction tube is employed, the sensitivity and the degree of accuracy of measurement is improved.
Brief description of the drawings
Fig. 1 is the overall structure diagram of optical detection apparatus of the present utility model；
Fig. 2 is the operation principle schematic diagram of the deflecting plate of the utility model optical detection apparatus；And
Fig. 3 is the structural representation of the fluorescent collecting part of the utility model optical detection apparatus.
Embodiment
Exemplary embodiment of the present utility model, feature and aspect are described in detail below with reference to accompanying drawing.It is identical in accompanying drawing
Reference represent the same or analogous element of function.Although the various aspects of embodiment are shown in the drawings, remove
Non-specifically is pointed out, it is not necessary to accompanying drawing drawn to scale.
Specifically, as shown in figure 1, the utility model provides a kind of optical detection apparatus of real-time fluorescent PCR amplification instrument, bag
Include：For sending the light emitting module of exciting light, scan module and detection module for receiving fluorescence；Wherein, light emitting module
Including the light source 10 being disposed adjacent, aperture 11, the first optical filter 12 and speculum 13；Scan module includes controller 20, refraction
Plate 21, object lens 22 and drive mechanism 23, object lens 22 are placed in the surface of specimen mount 50, and deflecting plate is placed in the top of object lens 22,
It is preferred that, light source be laser, xenon lamp, LED or Halogen lamp LED, deflecting plate be glass plate, plastic board or resinous flat, or
Other transparent material plates, also, in order to not weaken light intensity, it is preferred that the upper and lower surface of deflecting plate is coated with anti-reflection film, driving machine
Structure 23 is used to drive deflecting plate to rotate, and controller 20 is communicated to connect with drive mechanism 23, it is preferred that drive mechanism includes stepping electricity
Machine, controller 20 sends instruction to stepper motor, and stepper motor drives deflecting plate using the trunnion axis of deflecting plate as axle according to instruction
Rotate；Detection module includes photodetector 30, array of photoswitch 34 and fibre bundle 33, and fibre bundle 33 includes many and sample bottom
The one-to-one optical fiber of multiple plate holes in seat 50, the fluorescence that optical fiber is used to send in sample in plate hole is transmitted to photodetection
Device, array of photoswitch is connected with fibre bundle, for being controlled to the fluorescence transmission in every optical fiber；Controller and photoswitch
Array communications are connected, the break-make for controlling array of photoswitch.To improve measurement effect, it is preferred that detection module includes second
Optical filter 31, the first optical filter 12 and the second optical filter 31 are adjustable optical filter, the first optical filter 12 and the second optical filter 31
It is respectively used to filter to exciting light and fluorescence.
As Figure 1-3, exciting light is sent from light source 10, sequentially passes through aperture 11, the first optical filter 12 and speculum 13
The deflecting plate 21 of scan module is incided afterwards.Exciting light is incident from the upper surface of deflecting plate 21, after the refraction of deflecting plate from
The lower surface outgoing of deflecting plate, emergent light 02 (from the exciting light of deflecting plate lower surface outgoing) is with incident light 01 (from deflecting plate
The incident exciting light in surface) it is parallel, but the translation in horizontal direction is generated therebetween, with the change of incidence angle θ, translation
L is measured with change, incident light 01 remains vertical direction, with the rotation of deflecting plate, and incidence angle is constantly changing, because
This emergent light is constantly translated relative to incident light, is so achieved that the scanning motion of emergent light in the horizontal plane.It is excellent
Choosing, when changing due to incidence angle within 25 degree, translational movement keeps the linear changing relation with incidence angle, therefore, deflecting plate
The angle rotated around its trunnion axis is preferably limited within ± 25 degree.
As Figure 1-3, specimen mount 50 includes multiple plate holes, for example, can be 96 holes (such as conventional 8 × 12), each
A by-reaction pipe 51 can be accommodated in plate hole, sample to be tested is placed in reaction tube, emergent light 02 by object lens 22 focusing after,
The exciting light 03 being radiated in reaction tube 51 is formd, in the presence of exciting light 03, with PCR cycle, the sample in reaction tube
Originally fluorescence 04 is sent, the fluorescence is received by the optical fiber positioned at reaction tube side wall, optical fiber again transmits the fluorescence to photodetection
Device 30.It is preferred that, the multifiber in light shafts includes fibre-optical splice 32, and fibre-optical splice is the end equipment of optical fiber, optical fiber
Joint is used to optical fiber being fixed in the plate hole of specimen mount, and fibre-optical splice is fixed on specimen mount by structures such as screw threads
Plate hole in, when fibre-optical splice 31 is fixed in plate hole, the incident end face of its optical fiber fixed and reaction tube 51 can be made
Side wall is relative, the fluorescence sent so as to collecting fiber from side wall.With the rotation of deflecting plate, emergent light 02 is swept in the horizontal plane
Retouch, the exciting light 03 that emergent light is formed after the focusing of object lens 22 carries out horizontal sweep to reaction tube 51, using progressive scan
Form, is finally completed the scanning work to i.e. all reaction tubes of whole plate.On the one hand control drive mechanism 23 drives refraction to controller
Plate 21 is rotated, and is realized progressive scan of the exciting light 03 to reaction tube 51, is on the other hand also controlled array of photoswitch 34, make light
The corresponding optical fiber of that row reaction tube 51 that switch arrays 34 are being scanned to exciting light 03 is turned in sequence, and then will
These fiber laser arrays to fluorescence be transferred to photodetector successively and measure.
Compared with prior art, the utility model has the advantages that：
Single unit system only needs to control the movement of one moving part of deflecting plate, you can realize that light beam is scanned, simple in construction, cost
It is low, it is easy to safeguard；It is that full plate can be achieved to swash only with a light source without setting independent light source one by one for reaction tube quantity
Hair, completes fluorescent PCR measurement, therefore the versatility of device is stronger；The mode of the lateral fluorescent collecting of reaction tube is employed, is improved
The sensitivity and the degree of accuracy of measurement.
Finally it should be noted that：Above-described each embodiment is merely to illustrate the technical solution of the utility model, rather than
It is limited；Although the utility model is described in detail with reference to the foregoing embodiments, one of ordinary skill in the art
It should be understood that：It can still modify to the technical scheme described in previous embodiment, or to which part or whole
Technical characteristic carries out equivalent substitution；And these modifications or substitutions, the essence of appropriate technical solution is departed from the utility model
The scope of each embodiment technical scheme.
Claims (9)
1. a kind of optical detection apparatus of real-time fluorescent PCR amplification instrument, it is characterised in that including：Hair for sending exciting light
Optical module, scan module and the detection module for receiving fluorescence；
The light emitting module includes light source, aperture, the first optical filter and the speculum being disposed adjacent, and the exciting light is sent out from light source
Go out, the scan module is incided after sequentially passing through aperture, the first optical filter and speculum；
The scan module include deflecting plate, controller, drive mechanism and object lens, the object lens be placed in specimen mount just on
Side, the deflecting plate is placed in the top of the object lens, and the drive mechanism is used to drive deflecting plate to rotate, the controller and institute
Drive mechanism communication connection is stated, the exciting light incides the deflecting plate after speculum reflects；
The detection module includes photodetector, array of photoswitch and fibre bundle, and the fibre bundle includes many and the sample
The one-to-one optical fiber of multiple plate holes in this base, the optical fiber is used for sample reaction tube is sent in the plate hole fluorescence
Transmit to the photodetector, the array of photoswitch is connected with fibre bundle, for being controlled to the fluorescence transmission in optical fiber
System；
The controller is communicated to connect with the array of photoswitch.
2. optical detection apparatus according to claim 1, it is characterised in that：
The drive mechanism includes stepper motor, and the controller is used to send instruction to stepper motor, and stepper motor is used for root
Deflecting plate is driven to be rotated around the trunnion axis of deflecting plate according to the instruction.
3. optical detection apparatus according to claim 2, it is characterised in that：
The deflecting plate is rotated around its trunnion axis in certain angle, the angle ranging from the normal of deflecting plate and the folder of vertical direction
Angle angle, the angle ranging from 0-25 degree.
4. optical detection apparatus according to claim 1, it is characterised in that：
The detection module also includes the second optical filter, and first optical filter and the second optical filter are adjustable optical filter, point
The exciting light and fluorescence Yong Yu not filtered.
5. optical detection apparatus according to claim 1, it is characterised in that：
The multifiber includes fibre-optical splice, and the fibre-optical splice is used to optical fiber being fixed in the plate hole of specimen mount,
The fibre-optical splice is fixed in the plate hole of specimen mount by helicitic texture.
6. optical detection apparatus according to claim 5, it is characterised in that：
The fibre-optical splice is located in the plate hole, and optical fiber can be made relative with the side wall of sample reaction tube, gathers from the side
The fluorescence that wall is sent.
7. optical detection apparatus according to claim 1, it is characterised in that：
The deflecting plate is glass plate, plastic board or resinous flat.
8. optical detection apparatus according to claim 7, it is characterised in that：
The upper and lower surface of deflecting plate is coated with anti-reflection film.
9. optical detection apparatus according to claim 1, it is characterised in that：
The light source is laser, xenon lamp, LED or Halogen lamp LED.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720014415.3U CN206362706U (en) | 2017-01-06 | 2017-01-06 | A kind of optical detection apparatus of real-time fluorescent PCR amplification instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720014415.3U CN206362706U (en) | 2017-01-06 | 2017-01-06 | A kind of optical detection apparatus of real-time fluorescent PCR amplification instrument |
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| Publication Number | Publication Date |
|---|---|
| CN206362706U true CN206362706U (en) | 2017-07-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720014415.3U Expired - Fee Related CN206362706U (en) | 2017-01-06 | 2017-01-06 | A kind of optical detection apparatus of real-time fluorescent PCR amplification instrument |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108181239A (en) * | 2018-02-07 | 2018-06-19 | 张哲夫 | A kind of optical system of multichannel fluorescence quantitative PCR instrument |
| CN110184175A (en) * | 2018-02-22 | 2019-08-30 | 致茂电子(苏州)有限公司 | Automate fluorescence detecting system |
| CN110669655A (en) * | 2019-09-30 | 2020-01-10 | 浙江大学 | Disposable buckle type impedance and fluorescence method rapid gene detection device |
| CN110967324A (en) * | 2019-10-14 | 2020-04-07 | 广东省测试分析研究所（中国广州分析测试中心） | Optical detection device of multi-channel real-time fluorescence detector |
| CN111304050A (en) * | 2020-02-14 | 2020-06-19 | 深圳麦科田生物医疗技术有限公司 | Nucleic acid detecting device |
| WO2021169071A1 (en) * | 2020-02-25 | 2021-09-02 | 杭州博日科技有限公司 | Optical path position calibration method, calibration tool, and fluorescence quantitative detection system |
| CN116297416A (en) * | 2023-05-22 | 2023-06-23 | 科美诊断技术股份有限公司 | Photo-excitation chemiluminescence detection device and detection method |
-
2017
- 2017-01-06 CN CN201720014415.3U patent/CN206362706U/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108181239A (en) * | 2018-02-07 | 2018-06-19 | 张哲夫 | A kind of optical system of multichannel fluorescence quantitative PCR instrument |
| CN108181239B (en) * | 2018-02-07 | 2023-09-12 | 张哲夫 | Optical system of multichannel fluorescence quantitative PCR instrument |
| CN110184175A (en) * | 2018-02-22 | 2019-08-30 | 致茂电子(苏州)有限公司 | Automate fluorescence detecting system |
| CN110669655A (en) * | 2019-09-30 | 2020-01-10 | 浙江大学 | Disposable buckle type impedance and fluorescence method rapid gene detection device |
| CN110669655B (en) * | 2019-09-30 | 2021-07-06 | 浙江大学 | Disposable buckle type impedance and fluorescence method rapid gene detection device |
| CN110967324A (en) * | 2019-10-14 | 2020-04-07 | 广东省测试分析研究所（中国广州分析测试中心） | Optical detection device of multi-channel real-time fluorescence detector |
| CN111304050A (en) * | 2020-02-14 | 2020-06-19 | 深圳麦科田生物医疗技术有限公司 | Nucleic acid detecting device |
| WO2021169071A1 (en) * | 2020-02-25 | 2021-09-02 | 杭州博日科技有限公司 | Optical path position calibration method, calibration tool, and fluorescence quantitative detection system |
| CN116297416A (en) * | 2023-05-22 | 2023-06-23 | 科美诊断技术股份有限公司 | Photo-excitation chemiluminescence detection device and detection method |
| CN116297416B (en) * | 2023-05-22 | 2023-11-10 | 科美诊断技术股份有限公司 | Photo-excitation chemiluminescence detection device and detection method |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20170728 Termination date: 20210106 |