CN219578158U

CN219578158U - Stem cell is storage device for freezing

Info

Publication number: CN219578158U
Application number: CN202320543120.0U
Authority: CN
Inventors: 段鑫; 张淼; 彭娟; 寇大莲; 詹燕玲
Original assignee: Chengdu Kangjing Biotechnology Co ltd
Current assignee: Chengdu Kangjing Biotechnology Co ltd
Priority date: 2023-03-20
Filing date: 2023-03-20
Publication date: 2023-08-25
Anticipated expiration: 2033-03-20

Abstract

The utility model discloses a storage device for freezing stem cells, which comprises a pipe body with two open ends, wherein the pipe body is formed by sequentially communicating a plurality of spherical pipes with arc pipe walls, one end of the pipe body is provided with an injection port, the other end of the pipe body is provided with a liquid outlet, the side wall of the pipe body near one end of the liquid outlet is provided with an exhaust hole, a detachable cover body is arranged on the liquid outlet, and a filtering membrane is arranged between the exhaust hole and the liquid outlet. In the utility model, the tube body is arranged in a continuous spherical shape, so that the stem cells can be extremely frozen and uniformly cooled, and the cell recovery efficiency is high; simultaneously, the liquid outlet at the bottom of the tube body is sealed by the cover body, and the filtering membrane is arranged in the tube body, so that stem cells in the tube body can be cleaned after the cells are recovered, a centrifugal machine is avoided, and the operation steps are simple.

Description

Stem cell is storage device for freezing

Technical Field

The utility model relates to a storage device for freezing stem cells, in particular to a freezing tube suitable for storing stem cells by liquid nitrogen, and belongs to the technical field of medical appliances.

Background

The stem cell is taken as a totipotent cell of the human body, also called a seed cell, and has wide clinical application prospect because of the multidirectional differentiation potential. Therefore, it is necessary to develop a stable and efficient stem cell storage device. Currently, in order to better maintain the original cell characteristics and activity of stem cells during storage, the most traditional slow freezing/fast heating mode is generally adopted. The stem cells are stored in liquid nitrogen at the temperature of minus 196 ℃ after being slowly frozen, so that the cells are temporarily separated from the growth state, and are taken out of the liquid nitrogen for rapid rewarming (resuscitating) application when needed. When the stem cells are frozen in a laboratory, a certain amount of freezing protective agent is needed to be added into the stem cells, the stem cells are re-suspended to be uniform, then the uniform cells are injected into a common cell freezing tube in the market, and the stem cells are put into a liquid nitrogen device for preservation after being slowly frozen according to a certain standard flow operation. After the stem cells are frozen in liquid nitrogen, the basic movement of the cells is stopped, but the stem cells can undergo the processes of freezing and rewarming during the freezing and the resuscitating, so that various indexes can be reduced compared with the prior indexes, the activity of the cells can be lost in a certain proportion, and the resuscitating efficiency is greatly reduced. In addition, since the cryopreservation protective agent is required to be added, centrifugation operation is required to be carried out during resuscitation when a common cryopreservation tube in the market is used, so that the protective agent in cells is removed, and clinical application can be realized. Therefore, improving the stem cell liquid nitrogen cryopreservation tube is important to improving the stem cell resuscitation efficiency and the convenience of cryopreservation and resuscitation operation.

The utility model patent with the publication number of CN218288641U discloses a freezing pipe convenient to store, which consists of an outer pipe, an outer pipe and an inner pipe which are sleeved in sequence from outside to inside, wherein the upper end of the outer wall of the inner pipe is in threaded connection with the inside of the outer pipe through a threaded collar, a plurality of through holes are formed in the lower end of the inner pipe, a cell filter membrane is arranged on the through holes, a pipe cap of the outer pipe is arranged on a pipe jacking thread of the outer pipe, the outer pipe is axially and longitudinally locked, the outer pipe and the inner pipe can be protected, and leakage caused by movement of the outer pipe and the inner pipe during transportation is avoided; the design of the through hole can rotate the threaded lantern ring to upwards rotate the inner tube when new culture solution cannot be added immediately after resuscitation, the cryoprotectant in the inner tube flows into the outer tube through the cell filter membrane on the through hole, and stem cells are left in the inner tube, so that the toxicity of the cryoprotectant to cells can be temporarily reduced. The freezing tube of the patent can solve the problem of separation of the cryoprotectant and the cells, but due to the adoption of a plurality of sleeves, when the freezing tube is placed into a liquid nitrogen environment for freezing, the cells in the inner tube are ensured to be rapidly cooled without reducing various indexes and activities of the cells, and further verification is still needed.

Disclosure of Invention

The utility model aims to provide a storage device for freezing stem cells, wherein the tube body of the storage device is arranged in a continuous spherical shape, so that the stem cells can be quickly frozen and cooled uniformly, and the cell activity is high; simultaneously, the liquid outlet at the bottom of the tube body is sealed by the cover body, and the filtering membrane is arranged in the tube body, so that stem cells in the tube body can be cleaned after the cells are recovered, a centrifugal machine is avoided, and the operation steps are simple.

The utility model is realized by the following technical scheme: the utility model provides a storage device is used in stem cell freezing, includes both ends open-ended body, and the body is formed by a plurality of pipe wall being curved bulb intercommunication in proper order, and the one end opening of body is the injection port, and the other end opening of body is the liquid outlet, and the exhaust hole is established to the body lateral wall of nearly liquid outlet one end, establishes detachable lid on the liquid outlet, establishes filtering membrane between exhaust hole and liquid outlet.

A connecting pipe is arranged between the spherical pipe opening near the liquid outlet and the liquid outlet, and the exhaust hole is arranged on the pipe wall of the connecting pipe.

And the filter membrane mounting piece comprises an annular pressing block, an annular bottom block and a push-pull piece, the filter membrane is detachably arranged between the annular bottom block and the annular pressing block, the annular bottom block and the annular pressing block are sleeved in the connecting pipe, and the push-pull piece is connected to the annular bottom block.

And a clamping structure for fixing the filtering membrane is arranged between the annular bottom block and the annular pressing block.

The push-pull piece is arranged along the direction from the annular bottom block to the liquid outlet.

An annular sealing ring is arranged on the contact surface between the outer circumference of the annular pressing block and the inner wall of the connecting pipe.

And a matched and sealed thread structure is further arranged between the inner wall of the cover body and the outer wall of the connecting pipe.

An annular sealing ring is arranged on the contact surface between the inner wall of the cover body and the connecting pipe.

Compared with the prior art, the utility model has the following advantages:

(1) The utility model adopts the tube body structure similar to the spherical condenser tube to freeze the cell liquid, can quickly freeze the cells in the tube in liquid nitrogen at the temperature of minus 196 ℃, ensures the consistency of the freezing of the cell liquid by the consistent and continuous size of the spherical structure of the tube body, can effectively ensure various indexes of the cell liquid after the freezing and recovery of the stem cells, and has high cell recovery efficiency.

(2) The utility model is easy to realize the cleaning of cells, and is different from the existing freezing tube in that the liquid outlet is arranged at the bottom of the tube body and is sealed by the cover body, and the filtering membrane is also arranged in the tube body, so that after the cells are frozen and recovered, the cover body can be removed, the protective agent in the tube body is filtered and washed, and the cleaned stem cells are trapped in the freezing tube by the filtering membrane and can be directly used without centrifugally separating the protective agent, so that the operation is convenient.

(3) The filtering membrane is fixed in a connecting pipe at one end of a liquid outlet of a pipe body through a filtering membrane mounting piece, the annular bottom block and the annular pressing block are matched for use, the filtering membrane is fixed through a clamping structure between the annular bottom block and the annular pressing block, when the filtering membrane is used, the pushing and pulling piece is applied with force, the filtering membrane mounting piece fixed by the filtering membrane is inserted into the connecting pipe from the liquid outlet, and then the cover body is rotated to seal the liquid outlet; after the cell is frozen and recovered, the cover body is rotated to keep the filtering membrane to filter cell liquid in the connecting pipe, and after the protective agent is filtered and washed, the push-pull piece can be pulled to take out the filtering membrane mounting piece, the filtering membrane is replaced for reuse, and the structure is simple and the use is convenient.

(4) In order to ensure the sealing effect of the tube body, sealing rings are respectively arranged on the contact surface of the filter membrane mounting piece and the connecting tube and the contact surface of the cover body and the connecting tube, so that the cell can be prevented from leaking into the tube body in the freezing process of liquid nitrogen to influence the cell performance and activity.

Drawings

FIG. 1 is an assembled schematic view of the present utility model.

FIG. 2 is a schematic diagram of the structure of the present utility model when the cells are frozen.

Fig. 3 is an enlarged schematic view of a shown in fig. 2.

FIG. 4 is a schematic diagram showing the structure of the present utility model when washing cells.

The device comprises a 1-pipe body, a 2-injection port, a 3-liquid outlet, a 4-exhaust hole, a 5-cover body, a 6-filtering membrane, a 7-connecting pipe, an 8-filtering membrane mounting piece, a 9-annular pressing block, a 10-annular bottom block, an 11-push-pull piece, a 12-thread structure, a 13-annular sealing ring and a 14-pull hook.

Detailed Description

The present utility model will be described in further detail with reference to examples, but embodiments of the present utility model are not limited thereto.

Examples:

the embodiment is a storage device for freezing stem cells, and the storage device is actually a freezing tube for storing stem cells, is not only suitable for long-term maintenance of stem cells in a liquid nitrogen environment, but also can meet the requirement of cleaning and use after cell resuscitating.

As shown in the structures of fig. 1 and fig. 2, the freezing tube of this embodiment mainly comprises a tube body 1, a filtering membrane 6, a filtering membrane mounting member 8 and a cover body 5, wherein the tube body 1 is formed by sequentially communicating a plurality of spherical tubes with arc tube walls, and is similar to a spherical condensation tube, so that the contact area between cells in the tube and liquid nitrogen can be increased, and the freezing uniformity can be maintained. On the basis of the spherical structure, openings are arranged at two ends of the tube body 1, the top port of the tube body is an injection port 2, and the injection structure of the existing freezing tube sold in the market can be adopted to meet the requirement of injecting cells into the tube body 1; for convenience in use of the filtering membrane 6, the spherical tube opening corresponding to the bottom of the tube body 1 extends downwards to form a connecting tube 7, the bottom opening of the connecting tube 7 is the liquid outlet 3, the exhaust hole 4 can be formed in the connecting tube 7, the filtering membrane 6 is fixed in the connecting tube 7 below the exhaust hole 4 through the filter membrane mounting piece 8, and the liquid outlet 3 is sealed through the cover body 5. Specifically, can be equipped with in lid 5 inner wall with connecting pipe 7 outer wall complex screw thread structure 12 realize lid 5 to the sealed of liquid outlet 3, also can be convenient for cell cryopreservation revive the back, open lid 5 and can realize the filtration and the cell cleaning of protectant.

In this embodiment, the filter membrane mount 8 functions to enable the filter membrane 6 to be disposed within the connection tube 7. As shown in fig. 3, the specific structure of the filter membrane mounting member 8 includes an annular pressing block 9, an annular bottom block 10 and a push-pull member 11, where the annular pressing block 9 and the annular bottom block 10 are hollow annular components, and are used in cooperation with each other and detachably fix the filter membrane 6 between them, where there are multiple detachable modes, such as two components that are mutually clamped/buckled, and the two components are respectively disposed on the mating end surfaces of the annular pressing block 9 and the annular bottom block 10, and when the two are clamped/buckled together, the filter membrane 6 is fixed between the two components. The filtering membrane 6 is used for filtering the protective agent in the cells, the material of the filtering membrane 6 can be selected according to the protective agent, and the condition that the protective agent can pass through the filtering membrane 6 is satisfied, and the cells are not filtered by the filtering membrane 6 can be satisfied. The annular bottom block 10 and the annular pressing block 9 can be sleeved in the connecting pipe 7, and the push-pull piece 11 is arranged on the annular bottom block 10. During the use, will be fixed by the filter membrane mounting 8 of filtration membrane 6 according to the arrow mark that fig. 1 shows inserts the liquid outlet 3 of connecting pipe 7, push-and-pull spare 11 locates the below of annular bottom block 10 promptly, the propelling movement of the filter membrane mounting 8 of being convenient for, also can set up the pull hook 14 of inwards buckling in the tip of push-and-pull spare 11, the convenience is taken out of filter membrane mounting 8 when being convenient for change filtration membrane 6.

In order to ensure the effective sealing of the freezing storage pipe in the long-term maintaining process, sealing structures can be arranged on the structures such as the filter membrane mounting part 8, the cover body 5 and the like, as shown in fig. 4, the contact surfaces of the outer circumference of the annular pressing block 9 and the inner wall of the connecting pipe 7, the contact surfaces of the outer circumference of the annular bottom block 10 and the inner wall of the connecting pipe 7 and the contact surfaces of the inner wall of the cover body 5 and the connecting pipe 7 are respectively provided with an annular sealing ring 13, the materials of which can be selected according to the freezing storage environment and the sealing effect, and the effective sealing and long-term use can be satisfied.

In the concrete structure of the freezing storage tube, the tube body 1 can be made of a USP VI-grade mixed material, the diameter of the filtering membrane 6 is less than or equal to 15 mu m, and when the tube body 1 adopts a 1.5mL specification, the outer diameter of the tube body is 10mm, and the height of the tube body is 47mm; when the tube 1 was 5mL in size, the outer diameter was 16mm and the height was 59mm. The structural schematic diagrams of the freezing tube shown in fig. 1 to 3 are only examples, and aim to illustrate the specific structure and the assembly relation of the freezing tube, the proportional relation of the freezing tube is not required, and the corresponding specification of the freezing tube is required to be used in actual production.

Before use, after the filter membrane 6 is placed in the filter membrane mounting member 8 and fixed, the filter membrane mounting member 8 and the cover 5 are sequentially combined in the manner shown in fig. 1, and the liquid outlet 3 of the pipe body 1 is sealed. As shown in fig. 2, in order to verify the sealing performance of the pipe body 1 and discharge the air of the pipe body 1, the air exhaust device is arranged on the air exhaust hole 4, and after the air in the pipe is exhausted, the air exhaust hole 4 is closed. Then, the injection port 2 is opened, stem cells and protective agent are injected into the tube body 1 according to the operation requirement, after the injection is completed, the injection port 2 is closed, and then the freezing tube is put into liquid nitrogen for freezing preservation.

During recovery, the freezing tube is taken out of liquid nitrogen, quickly rewarmed in a water bath at 37 ℃, after being melted, the freezing tube is transferred into a biosafety cabinet, as shown in fig. 4, a cover body 5 below a tube body 1 is opened, a protective agent in the tube body 1 is filtered out into a small container below the tube body through a filter membrane 6, the small container is taken down, freezing solution is poured out, after the freezing solution is washed twice by adding complete culture medium/physiological saline, the cover body 5 below the tube body 1 is closed, after the complete culture medium is added, the freezing solution is poured out for use after shaking for a plurality of times. The residual quantity of the cryopreservation protective agent can be reduced through multiple times of flushing, and the influence of a small quantity of residual cryopreservation protective agent on the subsequent cell culture is less.

The foregoing description is only a preferred embodiment of the present utility model, and is not intended to limit the present utility model in any way, and any simple modification, equivalent variation, etc. of the above embodiment according to the technical matter of the present utility model fall within the scope of the present utility model.

Claims

1. A storage device for stem cell freezing, characterized in that: including both ends open-ended body (1), body (1) are curved bulb by a plurality of pipe wall and communicate in proper order and form, and the one end opening of body (1) is injection port (2), and the other end opening of body (1) is liquid outlet (3), and exhaust hole (4) are established to body (1) lateral wall near liquid outlet (3) one end, establish detachable lid (5) on liquid outlet (3), establish filtration membrane (6) between exhaust hole (4) and liquid outlet (3).

2. The storage device for stem cell freezing according to claim 1, wherein: a connecting pipe (7) is arranged between the spherical pipe opening near the liquid outlet (3) and the liquid outlet (3), and the exhaust hole (4) is arranged on the pipe wall of the connecting pipe (7).

3. The storage device for stem cell freezing according to claim 2, wherein: establish filter membrane installed part (8) in connecting pipe (7), filter membrane installed part (8) are including annular briquetting (9), annular bottom block (10) and push-and-pull spare (11), and filter membrane (6) detachable locates between annular bottom block (10) and annular briquetting (9), in annular bottom block (10) and annular briquetting (9) cover locate connecting pipe (7), push-and-pull spare (11) are connected on annular bottom block (10).

4. A storage device for stem cell freezing according to claim 3, wherein: a clamping structure for fixing the filtering membrane (6) is arranged between the annular bottom block (10) and the annular pressing block (9).

5. A storage device for stem cell freezing according to claim 3, wherein: the push-pull piece (11) is arranged along the direction from the annular bottom block (10) to the liquid outlet (3).

6. A storage device for stem cell freezing according to claim 3, wherein: an annular sealing ring (13) is arranged on the contact surface between the outer circumference of the annular pressing block (9) and the inner wall of the connecting pipe (7).

7. The storage device for stem cell freezing according to claim 2, wherein: a thread structure (12) matched and sealed is also arranged between the inner wall of the cover body (5) and the outer wall of the connecting pipe (7).

8. The storage device for stem cell freezing of claim 7, wherein: an annular sealing ring (13) is arranged on the contact surface between the inner wall of the cover body (5) and the connecting pipe (7).