CN219557647U

CN219557647U - Mouse cerebrospinal fluid sampling device

Info

Publication number: CN219557647U
Application number: CN202320224488.0U
Authority: CN
Inventors: 董俊一
Original assignee: Shanghai Lanli Biotechnology Co ltd
Current assignee: Shanghai Lanli Biotechnology Co ltd
Priority date: 2023-02-16
Filing date: 2023-02-16
Publication date: 2023-08-22
Anticipated expiration: 2033-02-16

Abstract

The utility model discloses a mouse cerebrospinal fluid sampling device, which belongs to the technical field of animal test instruments, and comprises: the operation panel, two sets of fixed subassembly, mouse head fixation clamp, extraction apparatus, anesthesia apparatus, the operation panel is the rectangle, fixed subassembly sets up the both sides on the operation panel, mouse head fixation clamp sets up on the operation panel, extraction apparatus sets up on the side that fixed subassembly was kept away from to the operation panel, anesthesia apparatus is close to extraction apparatus setting on the operation panel. Can accomplish work such as mouse is fixed, anesthesia, extraction, can keep the mouse stable at extraction in-process health, reduce the risk of puncturing the mouse blood vessel, it is little to the mouse injury, reduces the death rate of mouse, reduces the risk of blood pollution, and extraction efficiency is high, labour saving and time saving.

Description

Mouse cerebrospinal fluid sampling device

Technical Field

The utility model relates to the technical field of animal test instruments, in particular to a mouse cerebrospinal fluid sampling device.

Background

Cerebrospinal fluid (Cerebrospinal Fluid, CSF) is a colorless transparent fluid produced by the choroid plexus on the top of the lateral, third and fourth ventricles, filling the ventricles, subarachnoid space and various cerebral cisterna and spinal central canal. In the central nervous system, cerebrospinal fluid circulates in a direction that can flow down into the subarachnoid space of the spinal canal, up through the cerebellar notch to the subarachnoid space of the cranial vertex, and through the arachnoid particles into the superior sagittal sinus. Along with the continuous generation and absorption of cerebrospinal fluid, the preparation has the functions of protecting, regulating and nourishing brain and spinal cord, maintaining pH value and metabolism, etc. Changes in the physicochemical properties of the cerebrospinal fluid and in the metabolites can be caused when the central nervous system is diseased or damaged. Therefore, cerebrospinal fluid detection has decisive significance for diagnosis, treatment and prognosis evaluation of central nervous system diseases or injuries, and also has important roles in research of brain functions, brain metabolism and pharmacodynamics. At present, cerebrospinal fluid detection has become one of the indispensable diagnosis and monitoring means for diseases such as central nervous system infectious diseases, tumors, leukemia, lymphomas, cerebral parasitic diseases and the like, and is also helpful for the diagnosis and treatment of central system injuries, cerebrovascular diseases, multiple sclerosis, demyelinating diseases and the like.

The mice have small size, easy control, quick growth and reproduction, convenient feeding and management, and definite quality standard, are cultivated into a large number of inbred lines, closed groups and genetically mutant animals, and are widely applied to various fields of biomedical research. However, because the cerebral ventricles and subarachnoid space of the mice are narrow, the internal blood vessels are rich, the blood vessels are easy to be damaged to cause bleeding, cerebrospinal fluid is difficult to collect, the mice are easy to die, and repeated collection cannot be performed. The prior art mainly extracts through syringe equipment, does not have fixed equipment, can't pinpoint, and damage mouse brain tissue easily to influence the extraction of cerebrospinal fluid.

Disclosure of Invention

The utility model aims at: the utility model provides a mouse cerebrospinal fluid sampling device solves above-mentioned unable fixed mouse, is unfavorable for accurate location to damage mouse brain tissue, influence mouse cerebrospinal fluid and cause the death of mouse easily, unable repeated problem of extracting cerebrospinal fluid.

The technical scheme is as follows: the utility model provides a mouse cerebrospinal fluid sampling device, comprising: the operation panel, two sets of fixed subassembly, mouse head fixation clamp, extraction equipment, anesthesia equipment, the operation panel is the rectangle, fixed subassembly sets up the both sides on the operation panel, mouse head fixation clamp sets up on the operation panel, in two sets of fixed subassembly outside intermediate positions, extraction equipment sets up on the operation panel and keeps away from the edge of fixed subassembly, is close to mouse head fixation clamp, anesthesia equipment is close to extraction equipment and sets up on the operation panel. The mouse is tightly propped and fixed through the fixing component, the head of the mouse is fixed through the mouse head fixing clamp, the anesthetic device is used for anesthetizing the mouse, and finally the extraction device is used for extracting the cerebrospinal fluid of the mouse.

Further, the above-mentioned mouse cerebrospinal fluid sampling device, the fixed subassembly includes: fixed block, connecting rod, screw rod, fixed plate, the vertical setting of fixed block is on the operation panel, according to operation panel central line symmetric distribution, both sides all are equipped with two fixed blocks, the connecting rod runs through fixed block intermediate position, the screw rod sets up in the connecting rod top, runs through fixed block intermediate position, be equipped with screw hole and through-hole on the fixed plate, connect the screw rod through the screw hole, connect the connecting rod through the through-hole. The screw rod is rotated to enable the fixing plate to prop against the mouse, and the mouse is pressed and fixed at the middle position of the operating platform. The screw rod can be manually rotated by a worker, and motor drive can be also arranged.

Further, the above-mentioned mouse cerebrospinal fluid sampling device, the mouse head fixation clamp includes: base, axis of rotation, rotation handle, mouse clamp, clamping piece, the base setting is on the operation panel, sets up the intermediate position in the outside of two sets of fixed subassembly, rotation axis connection is in the base outside, the rotation handle sets up and passes through spring coupling in the base top, and end-to-end connection is in the axis of rotation, the mouse clamp is semi-circular structure, end-to-end connection is in the axis of rotation, the clamping piece is semi-circular structure, is connected with the mouse clamp through the spring. The mouse clamp is lifted by pressing down the rotating handle, and after the rotating handle is released, the spring restores the prototype, and the upward elastic force enables the clamping piece to clamp the head of the mouse. The semicircular structures of the mouse clamp and the clamping piece are matched with the shape of the mouse, so that the clamping is more stable.

Further, the above-mentioned mouse cerebrospinal fluid sampling device, the extraction apparatus comprises: the device comprises a fixed support, an extractor, an extraction needle head and an extraction needle hose, wherein the fixed support is arranged on the outer side of a mouse head fixing clamp, the middle of the fixed support is opposite to the mouse head fixing clamp, a through hole is formed in the top of the fixed support, the extractor penetrates through the through hole in the top of the fixed support and is fixed on the fixed support, the tip of the extraction needle head is conical, the length of the extraction needle head is 50mm, the length of the tip of the extraction needle head is 3mm, the caliber of the tip of the extraction needle head is 0.1mm, and the extraction needle head is connected with the extractor through the extraction needle hose. The tiny needle head can effectively avoid damaging blood vessels, has small damage to mice, can reduce the death rate of the mice, and is favorable for collecting the same mice for multiple times.

Further, the above-mentioned mouse cerebrospinal fluid sampling device, the anesthesia apparatus comprises: the device comprises an anesthesia support, a group of anesthesia atomizing drums, an air guide box and an anesthesia mask, wherein the anesthesia support is arranged on an operation table near extraction equipment in a vertical mode, a support platform is arranged in the middle of the anesthesia support, the anesthesia atomizing drums are arranged on the support platform, the air guide box is arranged at the top end of the anesthesia support, the bottom of the air guide box is connected with the group of anesthesia atomizing drums through a ventilation hose, and the anesthesia mask is connected with the air guide box through the ventilation hose.

Further, in the mouse cerebrospinal fluid sampling device, a through hole is formed in the connecting rod, an extension tube is slidably connected in the through hole, and the outer diameter of the extension tube is consistent with the inner diameter of the connecting rod. The stability of the fixing component is improved.

Furthermore, according to the mouse cerebrospinal fluid sampling device, the extractor is a sterile 1ml glass tube injector, so that the cerebral spinal fluid blood pollution condition can be intuitively observed.

Furthermore, in the mouse cerebrospinal fluid sampling device, the extraction needle is a sterile disposable needle. The depth of the small brain medullary pool inserted into the mice is 1-1.5mm, and the extraction time is not more than 1 minute.

Furthermore, in the mouse cerebrospinal fluid sampling device, isoflurane is used in the anesthesia atomization tube, and inhalation anesthesia is performed through isoflurane. The anesthetic concentration was adjusted to 2%, and the mice were put on an anesthetic mask to keep the anesthetic gas flowing to the anesthetic mask, so that the mice remained anesthetized.

The technical scheme can be seen that the utility model has the following beneficial effects: the mouse cerebrospinal fluid sampling device provided by the utility model has reasonable structural design, the mouse fixing, anesthesia, extraction and other works can be completed by using the device, the fixing component and the mouse head fixing clamp can keep the body of the mouse stable in the extraction process, the mouse can be subjected to inhalation anesthesia to avoid the influence of injection anesthesia on cerebrospinal fluid, the small extraction needle head can effectively avoid puncturing the blood vessel of the mouse, the damage to the mouse is small, the death rate of the mouse is reduced, the risk of blood pollution is reduced, the extraction efficiency is high, and the time and labor are saved.

Drawings

FIG. 1 is a schematic diagram of a device for sampling cerebrospinal fluid of a mouse according to the utility model;

FIG. 2 is a schematic view of a fixing assembly according to the present utility model;

FIG. 3 is a schematic view of the structure of the mouse head fixing clip according to the present utility model;

FIG. 4 is a schematic diagram of the structure of the extraction device of the present utility model;

fig. 5 is a schematic view of the structure of the anesthesia apparatus of the present utility model.

In the figure: the device comprises an operation table 1, two groups of fixing components 2, a mouse head fixing clamp 3, an extraction device 4, an anesthesia device 5, a fixing block 21, a connecting rod 22, a screw 23, a fixing plate 24, an extension tube 221, a base 31, a rotating shaft 32, a rotating handle 33, a mouse clamp 34, a clamping piece 35, a fixing support 41, an extractor 42, an extraction needle 43, an extraction needle hose 44, an anesthesia support 51, a group of anesthesia atomizing drums 52, an air guide box 53, an anesthesia mask 54 and a support platform 511.

Detailed Description

Example 1

The utility model provides a mouse cerebrospinal fluid sampling device as shown in fig. 1, operation panel 1, two sets of fixed subassembly 2, mouse head fixation clamp 3, extraction apparatus 4, anesthesia apparatus 5, operation panel 1 is the rectangle, fixed subassembly 2 sets up the both sides on operation panel 1, mouse head fixation clamp 3 sets up on operation panel 1, in the outside intermediate position of two sets of fixed subassembly 2, extraction apparatus 4 sets up on operation panel 1 keep away from the edge of fixed subassembly 2, is close to mouse head fixation clamp 3, anesthesia apparatus 5 is close to extraction apparatus 4 and sets up on operation panel 1. The mouse is tightly propped and fixed through the fixing component 2, the head of the mouse is fixed through the mouse head fixing clamp 3, the anesthetic device 5 is used for anesthetizing the mouse, and finally the extraction device 4 is used for extracting cerebrospinal fluid of the mouse.

A mouse cerebrospinal fluid sampling device as shown in fig. 2, said fixing assembly 2 comprising: fixed block 21, connecting rod 22, screw rod 23, fixed plate 24, fixed block 21 is vertical to be set up on operation panel 1, according to operation panel 1 central line symmetric distribution, both sides all are equipped with two fixed blocks 21, connecting rod 22 runs through fixed block 21 intermediate position, screw rod 23 sets up in connecting rod 22 top, runs through fixed block 21 intermediate position, be equipped with screw hole and through-hole on the fixed plate 24, connect screw rod 23 through the screw hole, connect connecting rod 22 through the through-hole. The screw 23 is rotated to enable the fixing plate 24 to be abutted against the mouse, so that the mouse is pressed and fixed at the middle position of the operating platform 1.

A mouse cerebrospinal fluid sampling device as shown in fig. 3, said mouse head-fixing clip 3 comprising: base 31, axis of rotation 32, rotation handle 33, mouse clamp 34, clamping piece 35, base 31 sets up on operation panel 1, sets up the intermediate position in the outside of two sets of fixed subassembly 2, axis of rotation 32 is connected in the base 31 outside, rotation handle 33 sets up and passes through spring coupling in base 31 top, and end-to-end connection is on axis of rotation 32, mouse clamp 34 is semi-circular structure, end-to-end connection is on axis of rotation 32, clamping piece 35 is semi-circular structure, is connected with mouse clamp 34 through the spring. Pressing the rotating handle 33 lifts the mouse clamp 34, and after releasing the rotating handle 33, the spring returns to the original shape, and the upward elastic force enables the clamp 35 to clamp the head of the mouse. The semicircular structure of the mouse clip 34 and the clip 35 matches the mouse body shape, making the clip more stable.

A mouse cerebrospinal fluid sampling device as shown in fig. 4, said extraction apparatus 4 comprising: the device comprises a fixed support 41, an extractor 42, an extraction needle 43 and an extraction needle hose 44, wherein the fixed support 41 is arranged on the outer side of a mouse head fixing clamp 3, the middle of the fixed support is opposite to the mouse head fixing clamp 3, a through hole is formed in the top of the fixed support, the extractor 42 penetrates through the through hole in the top of the fixed support 41 and is fixed on the fixed support 41, the tip of the extraction needle 43 is conical, the length of the extraction needle 43 is 50mm, the length of the tip is 3mm, the caliber of the tip is 0.1mm, the extraction needle 43 and the extractor 42 are connected through the extraction needle hose 44, the extractor 42 is a sterile 1ml glass tube injector, the extraction needle 43 is a sterile disposable needle, and the depth of the extraction needle inserted into a mouse cerebellum medullary pool is 1-1.5mm, and the extraction time is not more than 1 minute. The extractor 42 glass structure can intuitively observe the cerebral spinal fluid blood pollution condition, the tiny needle head can effectively avoid damaging blood vessels, the damage to mice is small, the death rate of the mice can be reduced, and the same mice can be collected for multiple times.

A mouse cerebrospinal fluid sampling device as shown in fig. 5, said anesthesia apparatus 5 comprising: the device comprises an anesthesia support 51, a group of anesthesia atomizing drums 52, an air guide box 53 and an anesthesia mask 54, wherein the anesthesia support 51 is vertically arranged on the operation table 1 near the extraction equipment 4, a support platform 511 is arranged in the middle of the anesthesia support 51, the anesthesia atomizing drums 52 are arranged on the support platform 511, the air guide box 53 is arranged at the top end of the anesthesia support 51, the bottom of the air guide box 53 is connected with the group of anesthesia atomizing drums 52 through an air hose, and the anesthesia mask 54 is connected with the air guide box 53 through the air hose.

Example 2

Based on embodiment 1, in this embodiment, as shown in fig. 2, in a mouse cerebrospinal fluid sampling device, the fixing assembly 2 is controlled by rotation of a screw 23, the rotation of the screw can be manually performed by an operator, a driving motor can also be provided, a pressure sensor is provided at a side far away from the fixing plate 24, the driving motor drives the screw 23 to rotate, the fixing plate 24 compresses the mouse, and when the pressure sensor detects a certain pressure value, the driving motor is stopped.

In this embodiment, a through hole is formed in the connecting rod 22, and an extension pipe 221 is slidably connected in the through hole, and an outer diameter of the extension pipe 221 is identical to an inner diameter of the connecting rod 22.

In this embodiment, isoflurane is used in the anesthetic atomizing cylinder 52, and inhalation anesthesia is performed by isoflurane. The anesthetic concentration was adjusted to 2%, and the mice were kept anesthetized by wearing an anesthetic mask 54 to keep anesthetic gas flowing to the anesthetic mask 54.

It should be noted that the above description is only a technical solution of the utility model, and is not limiting. While the utility model has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents substituted for elements thereof without departing from the scope of the utility model, which is to be encompassed by the appended claims.

Claims

1. A mouse cerebrospinal fluid sampling device, characterized in that:

comprising the following steps: an operation table (1), wherein the operation table (1) is rectangular;

two groups of fixing components (2), wherein the fixing components (2) are arranged on two sides of the operating platform (1);

the mouse head fixing clamp (3) is arranged on the operation table (1) and is arranged at the middle position outside the two groups of fixing components (2);

the extraction device (4) is arranged on the side, away from the fixed component (2), of the operation table (1) and is close to the mouse head fixing clamp (3);

and the anesthesia device (5) is arranged on the operation table (1) close to the extraction device (4).

2. The mouse cerebrospinal fluid sampling device according to claim 1, wherein: the fixing assembly (2) comprises:

the fixed blocks (21) are vertically arranged on the operation table (1), and are symmetrically distributed according to the center line of the operation table (1), and two fixed blocks (21) are arranged on two sides of the operation table;

the connecting rod (22) penetrates through the middle position of the fixed block (21);

the screw rod (23) is arranged above the connecting rod (22) and penetrates through the middle position of the fixed block (21);

the fixing plate (24), be equipped with screw hole and through-hole on the fixing plate (24), connect screw rod (23) through the screw hole, connect connecting rod (22) through the through-hole.

3. The mouse cerebrospinal fluid sampling device according to claim 1, wherein: the mouse head fixing clip (3) comprises:

the base (31) is arranged on the operating platform (1) and is arranged at the middle position outside the two groups of fixed components (2);

a rotating shaft (32), wherein the rotating shaft (32) is connected to the outer side of the base (31);

the rotating handle (33) is arranged above the base (31) and connected with the rotating shaft (32) through a spring, and the tail end of the rotating handle (33) is connected with the rotating shaft (32);

the mouse clamp (34) is of a semicircular structure, and the tail end of the mouse clamp (34) is connected to the rotating shaft (32);

the clamping piece (35), the clamping piece (35) is of a semicircular structure, and is connected with the mouse clamp (34) through a spring.

4. The mouse cerebrospinal fluid sampling device according to claim 1, wherein: the extraction device (4) comprises:

the fixing support (41) is arranged at the outer side of the mouse head fixing clamp (3), the middle of the fixing support is opposite to the mouse head fixing clamp (3), and a through hole is formed in the top of the fixing support;

the extractor (42) passes through a through hole at the top of the fixed bracket (41) and is fixed on the fixed bracket (41);

and the tip of the extraction needle head (43) is conical, and the extraction needle head (43) is connected with the extractor (42) through an extraction needle hose (44).

5. The mouse cerebrospinal fluid sampling device according to claim 1, wherein: the anesthesia apparatus (5) comprises:

the anesthesia support (51) is vertically arranged on the operation table (1) close to the extraction equipment (4), and a support platform (511) is arranged in the middle of the anesthesia support (51);

a set of anesthetic atomizing barrels (52), wherein the anesthetic atomizing barrels (52) are arranged on a bracket platform (511);

the air guide box (53), the air guide box (53) is arranged at the top end of the anesthesia support (51), and the bottom of the air guide box (53) is connected with a group of anesthesia atomizing drums (52) through an air hose;

and the anesthetic mask (54) is connected with the air guide box (53) through an air hose.

6. A mouse cerebrospinal fluid sampling device according to claim 2, wherein: the connecting rod (22) is internally provided with a through hole, an extension pipe (221) is connected in a sliding manner in the through hole, and the outer diameter of the extension pipe (221) is consistent with the inner diameter of the connecting rod (22).

7. The mouse cerebrospinal fluid sampling device according to claim 4, wherein: the extractor (42) is a 1ml glass tube syringe.

8. The mouse cerebrospinal fluid sampling device according to claim 4, wherein: the extraction needle (43) is a sterile disposable needle.

9. The mouse cerebrospinal fluid sampling device according to claim 5, wherein: isoflurane is used in the anesthesia atomizing cylinder (52), and inhalation anesthesia is performed through isoflurane.