CN219540335U - Microfluidic chip capable of quantifying sample - Google Patents
Microfluidic chip capable of quantifying sample Download PDFInfo
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- CN219540335U CN219540335U CN202121153032.7U CN202121153032U CN219540335U CN 219540335 U CN219540335 U CN 219540335U CN 202121153032 U CN202121153032 U CN 202121153032U CN 219540335 U CN219540335 U CN 219540335U
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- microfluidic chip
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- sample
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- 238000001514 detection method Methods 0.000 claims abstract description 67
- 239000000758 substrate Substances 0.000 claims abstract description 59
- 238000006243 chemical reaction Methods 0.000 claims abstract description 40
- 238000002347 injection Methods 0.000 claims abstract description 31
- 239000007924 injection Substances 0.000 claims abstract description 31
- 239000012528 membrane Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 15
- 238000007789 sealing Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 4
- 239000000523 sample Substances 0.000 description 49
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 230000003321 amplification Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a microfluidic chip capable of quantifying a sample. The microfluidic chip is provided with a plurality of detection units, each detection unit forms an independent detection channel, the microfluidic chip comprises a substrate, the volumes of the detection channels are equal, and each detection unit at least comprises: a reaction chamber disposed in the substrate; the sample injection hole is arranged on the upper surface of the substrate; the sample injection channel is arranged in the substrate and is communicated with the reaction cavity and the sample injection hole; the volume of each detection channel at least comprises the volume of a sample injection channel of the volume of the reaction cavity. The utility model ensures that the sample injection amount of each detection channel is equal, and has simple structure and convenient operation.
Description
Technical Field
The utility model relates to a microfluidic chip capable of quantifying a sample, in particular to a nucleic acid amplification detection microfluidic chip.
Background
The microfluidic chip may be used as a biological, chemical microreactor or microsystem. The nucleic acid detection has the characteristics of high sensitivity and good specificity, and occupies extremely important positions in life science and medical examination. The microfluidic chip technology is combined with the nucleic acid detection technology, and the microfluidic chip which can be used for high-flux and full-automatic nucleic acid analysis and detection of biological samples is developed, so that the advantages of small reagent consumption, high analysis speed, high detection flux, high automation degree, non-professional use and the like are realized, and the method is particularly suitable for multi-index rapid quantitative detection of nucleic acid molecules in biological samples. For multiple detection assays in parallel (parallel assays, or multi-index assays), a multichannel microfluidic chip is often required, and ideally the amount of sample entering each detection channel should be equal. However, it is difficult for the existing microfluidic chip detection system to ensure that the amount of sample entering each detection channel is the same, and it is difficult to determine whether the amount of sample in each detection channel is the same. Although some detection systems are capable of quantifying sample into each detection channel, complex flow control mechanisms are required, and often the operation is complex.
Disclosure of Invention
In view of the above technical problems, the present utility model provides a microfluidic chip capable of quantifying a sample, which ensures that the sample injection amounts of all detection channels are equal, and has a simple structure and convenient operation.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a microfluidic chip having a plurality of detection units, each detection unit forming an independent detection channel, the microfluidic chip comprising a substrate, the detection channels having equal volumes, each detection unit comprising at least:
a reaction chamber disposed in the substrate;
the sample injection hole is arranged on the upper surface of the substrate; and
The sample injection channel is arranged in the substrate and is communicated with the reaction cavity and the sample injection hole;
the volume of each detection channel at least comprises the volume of a sample injection channel of the volume of the reaction cavity.
Preferably, each detection unit further comprises an exhaust hole communicated with the reaction cavity, the exhaust holes are formed in the upper surface of the substrate, and the microfluidic chip further comprises a gas permeable membrane which does not allow liquid to pass through, and the gas permeable membrane covers the exhaust holes.
More preferably, the gas permeable membrane is a PTFE membrane.
More preferably, the ventilation film is attached to the upper surface of the substrate, the exhaust hole is opened on a part of the upper surface of the substrate covered by the ventilation film, and the sample introduction hole is opened on a part of the upper surface of the substrate not covered by the ventilation film.
More preferably, each of the detection units further includes a liquid outlet channel provided in the substrate and communicating the reaction chamber and the exhaust hole, and the volume of each of the detection channels further includes a liquid outlet channel thereof.
Further, the reaction chambers are arranged on one side of the substrate at intervals along the long side direction or the short side direction of the substrate in parallel, the volumes of the sample introduction channels are equal, the volumes of the reaction chambers are equal, and the volumes of the liquid outlet channels are equal.
Further, each sample injection hole is arranged in the middle area of the front surface of the substrate, the sample injection channels have different shapes in a top view, and the aperture and the length of each sample injection channel are equal.
Further, the sample injection holes are arranged at intervals along a straight line.
More preferably, a reagent is placed in the reaction chamber.
Preferably, the microfluidic chip further includes a sealing film capable of covering an upper surface of the substrate to seal each of the detection channels, and/or a heating film capable of covering a lower surface of the substrate to heat each of the detection channels.
Compared with the prior art, the utility model has the following advantages:
the microfluidic chip is characterized in that a plurality of detection channels are arranged on a substrate in parallel, the volumes of the detection channels are equal, the sample quantity added into each detection channel can be ensured to be definite and equal when a multi-index test or a parallel test is carried out, the reacted sample can be accurately quantified, and meanwhile, the microfluidic chip is simple in structure and convenient to operate.
Drawings
In order to more clearly illustrate the technical solutions of the present utility model, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic perspective view of a microfluidic chip according to an embodiment of the present utility model;
FIG. 2 is a partially exploded schematic view of the microfluidic chip of FIG. 1;
FIG. 3 is a schematic view of a process of attaching a seal to the microfluidic chip shown in FIG. 1;
FIG. 4 is a schematic diagram of each detection channel;
fig. 5 is a schematic view of the lower surface of the microfluidic chip shown in fig. 1.
Wherein,,
1. a substrate; 2. a breathable film; 3. sealing film; 4. heating the film;
10. a detection unit; 101. a sample inlet; 102. a sample introduction channel; 103. a reaction chamber; 104. a liquid outlet channel; 105. and an exhaust hole.
Detailed Description
Preferred embodiments of the present utility model will be described in detail below with reference to the attached drawings so that the advantages and features of the present utility model can be more easily understood by those skilled in the art. The description of these embodiments is provided to assist understanding of the present utility model, but is not intended to limit the present utility model.
According to an embodiment of the present utility model, a microfluidic chip, in particular, a microfluidic chip for nucleic acid amplification detection is provided. Referring to fig. 1 to 5, the microfluidic chip includes a substrate 1. The microfluidic chip has a plurality of detection units 10 juxtaposed on a substrate 1, each detection unit 10 forming an independent detection channel. The volumes of the detection channels are equal, and each detection unit 10 includes: the volume of each detection channel is specifically the sum of the volume of the sample inlet channel 102, the volume of the reaction chamber 103 and the volume of the liquid outlet channel 104, and the sample inlet hole 101, the sample inlet channel 102, the reaction chamber 103, the liquid outlet channel 104 and the gas outlet hole 105. The reaction chambers 103 are provided in the substrate 1, in which the solid reagent is stored in advance, and in this embodiment, the reaction chambers 103 are arranged in parallel at intervals along the short side direction of the substrate 1, and in other embodiments, the reaction chambers 103 may be arranged in parallel at intervals along the long side direction of the substrate 1, which is not limited herein. The sample injection hole 101 is arranged on the upper surface of the substrate 1, and the sample injection channel 102 is arranged in the substrate 1 and is communicated with the reaction cavity 103 and the sample injection hole 101. The exhaust hole 105 is provided on the upper surface of the substrate 1, and the liquid outlet passage 104 is provided in the substrate 1 and communicates the reaction chamber 103 with the exhaust hole 105. The volume of each detection channel is equal, so that the sample quantity entering each detection channel is ensured to be equal, and the samples participating in the reaction can be accurately quantified.
The microfluidic chip further includes a gas permeable membrane 2 that does not allow liquid to pass through, and the gas permeable membrane 2 covers the gas vent 105. Specifically, the gas permeable membrane 2 is attached to the upper surface of the substrate 1, the gas vent 105 is opened at a portion of the upper surface of the substrate 1 covered with the gas permeable membrane 2, and the sample inlet 101 is opened at a portion of the upper surface of the substrate 1 not covered with the gas permeable membrane 2. The gas permeable membrane 2 is capable of allowing gas to pass through but not liquid, and may be a PTFE (polytetrafluoroethylene ) membrane. In the sample adding process, the gas in the detection channel can be discharged through the breathable film 2, so that bubbles are prevented from being formed in the detection channel; when the sample flows to the vent hole 105 and contacts the air permeable membrane 2, the air permeable membrane 2 is cut off, which indicates that the sample in the detection channel reaches a set amount, and the sample injection is stopped.
In this embodiment, the volumes of the sample introduction channels 102 and the reaction chambers 103 are equal, and the volumes of the liquid outlet channels 104 are equal. As shown in fig. 4, the distribution of the detection channels on the substrate 1 is that the reaction chambers 103 are arranged on one side of the substrate 1 at intervals along the short side direction of the substrate 1, and the parts of the substrate 1 corresponding to the reaction chambers 103 (specifically, the parts above the reaction chambers 103 and near the upper edge side of the substrate 1) are made of light-transmitting materials so as to facilitate fluorescence detection. Each sample introduction hole 101 is centrally provided at a central region of the front surface of the substrate 1 so as to facilitate sample introduction. Thus, in consideration of the convenience of sample injection and the convenience of fluorescence detection, the shape of the sample injection channel 102 is different in a top view, and the sample injection channel 102 is composed of a straight line segment and an inclined line segment or a straight line segment and a curved line segment extending in the longitudinal direction of the substrate 1. In this embodiment, the apertures of the sample channels 102 are equal and the lengths are equal, and specifically, the total lengths of the sample channels 102 are equal by adjusting the ratio of the length of the straight line segment to the length of the oblique line segment/curved line segment.
Further, the plurality of injection holes 101 are arranged at intervals along a straight line, and the plurality of exhaust holes 105 are also arranged at intervals along a straight line. The straight line extends in particular in the short-side direction of the substrate 1.
The microfluidic chip further includes a sealing film 3 capable of covering the upper surface of the substrate 1 to seal each detection channel, the sealing film 3 being made of a gas-impermeable material. After sample injection is completed, the sealing film 3 is attached to the upper surface of the substrate 1 and the ventilated membrane 2, the sample injection hole 101 and the vent hole 105 are closed, external air is prevented from entering the detection channel, and a closed detection system is provided. Each reaction chamber 103 is not covered with the sealing film 3.
The microfluidic chip further comprises a heating film 4 which can be coated on the lower surface of the substrate 1 to heat each detection channel, and after the detection channels are electrified, the heating film 4 heats up to directly heat the reaction system in the reaction cavity 103 above the heating film, so that the amplification reaction process can be accelerated.
In the embodiment, the solid reagent is stored in the reaction cavity 103, the sample is added from each sample injection hole 101 through a dropper, and the sealing film 3 is attached after the sample addition is finished, so that the operation is simple, the quantitative accuracy is realized, and the reaction is quick; the reacted sample can be accurately quantified. The substrate 1 is attached with the heating film 4, so that the amplification reaction process can be accelerated. The sample injection holes 101 on the substrate 1 are attached through the breathable film 2, so that the internal air of the reaction holes can be completely discharged, and bubbles are avoided.
As used in this specification and in the claims, the terms "comprises" and "comprising" merely indicate that the steps and elements are explicitly identified, and do not constitute an exclusive list, as other steps or elements may be included in a method or apparatus. The term "and/or" as used herein includes any combination of one or more of the associated listed items.
It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly or indirectly fixed or connected to the other feature. Further, the descriptions of the upper, lower, left, right, etc. used in the present utility model are merely with respect to the mutual positional relationship of the constituent elements of the present utility model in the drawings.
The above-described embodiments are provided for illustrating the technical concept and features of the present utility model, and are intended to be preferred embodiments for those skilled in the art to understand the present utility model and implement the same according to the present utility model, not to limit the scope of the present utility model. All equivalent changes or modifications made according to the principles of the present utility model should be construed to be included within the scope of the present utility model.
Claims (9)
1. A microfluidic chip having a plurality of detection units, each detection unit forming an independent detection channel, the microfluidic chip comprising a substrate, wherein the detection channels have equal volumes, each detection unit comprising at least:
a reaction chamber disposed in the substrate;
the sample injection hole is arranged on the upper surface of the substrate; and
The sample injection channel is arranged in the substrate and is communicated with the reaction cavity and the sample injection hole;
the volume of each detection channel at least comprises the volume of a sample injection channel of the volume of a reaction cavity of the detection channel; each detection unit further comprises an exhaust hole communicated with the reaction cavity, the exhaust holes are formed in the upper surface of the substrate, the microfluidic chip further comprises a breathable film which does not allow liquid to pass through, and the breathable film covers the exhaust holes.
2. The microfluidic chip according to claim 1, wherein the gas permeable membrane is a PTFE membrane.
3. The microfluidic chip according to claim 1, wherein the gas permeable membrane is attached to the upper surface of the substrate, the gas vent is opened on a portion of the upper surface of the substrate covered with the gas permeable membrane, and the sample inlet is opened on a portion of the upper surface of the substrate not covered with the gas permeable membrane.
4. The microfluidic chip according to claim 1, wherein each of the detection units further comprises a liquid outlet channel provided in the substrate and communicating the reaction chamber and the exhaust hole, and the volume of each of the detection channels further comprises a liquid outlet channel thereof.
5. The microfluidic chip according to claim 4, wherein the reaction chambers are arranged on one side of the substrate at intervals in a longitudinal direction or a short direction of the substrate, the sample introduction channels have equal volumes, the reaction chambers have equal volumes, and the liquid outlet channels have equal volumes.
6. The microfluidic chip according to claim 5, wherein each of the sample introduction holes is provided at a central region of the front surface of the substrate, the sample introduction channels are different in shape in a top view, and the sample introduction channels are equal in aperture and length.
7. The microfluidic chip according to claim 6, wherein the sample injection holes are arranged at intervals along a line.
8. The microfluidic chip according to claim 1, wherein a reagent is placed in the reaction chamber.
9. The microfluidic chip according to claim 1, further comprising a sealing film capable of covering an upper surface of the substrate to seal each of the detection channels, and/or a heating film capable of covering a lower surface of the substrate to heat each of the detection channels.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121153032.7U CN219540335U (en) | 2021-05-25 | 2021-05-25 | Microfluidic chip capable of quantifying sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121153032.7U CN219540335U (en) | 2021-05-25 | 2021-05-25 | Microfluidic chip capable of quantifying sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219540335U true CN219540335U (en) | 2023-08-18 |
Family
ID=87735150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121153032.7U Active CN219540335U (en) | 2021-05-25 | 2021-05-25 | Microfluidic chip capable of quantifying sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219540335U (en) |
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2021
- 2021-05-25 CN CN202121153032.7U patent/CN219540335U/en active Active
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