CN219333612U - Instrument for removing pollution of nucleic acid aerosol - Google Patents
Instrument for removing pollution of nucleic acid aerosol Download PDFInfo
- Publication number
- CN219333612U CN219333612U CN202223043959.2U CN202223043959U CN219333612U CN 219333612 U CN219333612 U CN 219333612U CN 202223043959 U CN202223043959 U CN 202223043959U CN 219333612 U CN219333612 U CN 219333612U
- Authority
- CN
- China
- Prior art keywords
- air
- nucleic acid
- scavenger
- acid aerosol
- air outlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000443 aerosol Substances 0.000 title claims abstract description 87
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 66
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 66
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 66
- 239000007921 spray Substances 0.000 claims abstract description 51
- 239000002516 radical scavenger Substances 0.000 claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 238000003860 storage Methods 0.000 claims abstract description 22
- 238000001179 sorption measurement Methods 0.000 claims description 46
- 239000011941 photocatalyst Substances 0.000 claims description 24
- 238000011109 contamination Methods 0.000 claims description 22
- 238000000746 purification Methods 0.000 claims description 21
- 230000001681 protective effect Effects 0.000 claims description 16
- 230000002572 peristaltic effect Effects 0.000 claims description 11
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 abstract description 6
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 13
- 238000004140 cleaning Methods 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 238000001816 cooling Methods 0.000 description 7
- 238000003825 pressing Methods 0.000 description 7
- 230000003321 amplification Effects 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 238000010926 purge Methods 0.000 description 6
- 238000005507 spraying Methods 0.000 description 6
- 239000002245 particle Substances 0.000 description 5
- 239000012459 cleaning agent Substances 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000003595 mist Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 238000000889 atomisation Methods 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000013032 photocatalytic reaction Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000034809 Product contamination Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a nucleic acid aerosol pollution removal instrument, which comprises an outer shell, wherein an air inlet and an air outlet are formed in the outer shell, a liquid storage tank for storing a nucleic acid aerosol scavenger, a spray head, an air duct and a fan in the air duct are arranged in the outer shell, the fan can drive air flow of an external environment to flow into the air duct through the air inlet and flow out of the air outlet, the scavenger in the liquid storage tank can be pumped to the spray head to form dry fog to spray, and the sprayed dry fog can flow along with the air flow flowing out of the air outlet. The dry fog is circularly sent to each corner of the space where the nucleic acid aerosol pollution remover is located along with the air flow formed under the driving of the fan, so that the scavenger is more micronized and equalized, the scavenger can more fully capture the PCR aerosol in the air and efficiently destroy the conjugated double bonds of purine and pyrimidine bases of DNA, thereby efficiently completing the purpose of removing the nucleic acid pollution in a PCR laboratory.
Description
Technical Field
The utility model belongs to the field of design of a nucleic acid aerosol pollution eliminating device, and particularly relates to a nucleic acid aerosol pollution eliminating instrument.
Background
PCR detection is widely used because of its advantages of high sensitivity, rapidness, convenient operation, etc., but due to its extremely high sensitivity, very small amounts of contamination sources may cause false positives in the detection results. In the PCR experiment, the particle size of the nucleic acid aerosol suspended in the laboratory is generally 0.001 μm to 1000 μm, the reaction tube is relatively vigorously shaken during operation, the nucleic acid aerosol can be formed during the opening of the cover, the sample suction and the repeated sample suction of the pipettor, and the nucleic acid aerosol is widely existing on the laboratory table, the biosafety counter table, the pipettor, the door handle, the PCR instrument, the centrifuge and the like along with the intensity of the experiment and the time, and the copy amount of the PCR amplified product is large (generally 10 13 copy/mL), far above the limit of PCR detection-several copies, so that minimal contamination of PCR products can form false positives.
PCR contamination is generally classified into four categories: 1. cross contamination between samples, mainly in the process of sampling (sampling), is caused by the cross contamination between sampling tools; or contamination between samples due to improper use of pipettes, centrifuge tubes, etc. during nucleic acid extraction; pollution of PCR reagent, mainly in the course of preparing PCR reagent, because of pipettor, container, negative control and other reagents are polluted by nucleic acid template or positive control; 3. the pollution of the cloned plasmid often uses positive references in laboratory operation, most of the positive references are made of some cloned plasmids, the concentration of the cloned plasmids in unit volume is high, and the pollution is easily caused by improper use; PCR amplification product contamination, which is the most dominant, common and most headache-causing contamination problem in PCR reactions. Because the copy number of the PCR product is large and far higher than the limit of detecting a plurality of copies by PCR, the pollution of the trace PCR product can cause false positive results.
The most difficult to eliminate of nucleic acid contamination in PCR laboratories is aerosol contamination, especially of amplified productsAnd (5) dyeing. Contamination mainly occurs during the amplification and product analysis stages of PCR because the copy number of PCR products is large (typically 10 13 copy/mL), aerosol is formed when the liquid surface of the amplified product rubs with air, so aerosol contamination can be formed by relatively violent shaking of the reaction tube, sample suction during uncapping, and repeated sample suction. The splashed amplification products float in the air as aerosols, adhere to the object surface and to the body of the test personnel, and contaminate the entire laboratory as personnel and materials flow. The situation is improved if the pollution requires many days of laboratory vacation and active and effective treatment measures are taken.
At present, the first step of pollution in the experiment needs to pause the experiment, and the influence of false positives on the past detection result is evaluated. Thoroughly clean and clear up the laboratory, change relevant reagent consumptive material as far as possible, clean instrument and equipment and remove nucleic acid treatment, operating personnel bathes and trades clothes. The traditional processing method comprises the following steps: chemical treatment methods generally have carcinogenicity, interfere with detection of amplification products, have low efficiency for controlling G+C-rich and short amplification products, have unstable efficiency, and the like. Ultraviolet lamp irradiation method: the method is effective only for long fragments with the length of more than 300bp, and has little effect on short fragments. Also, the window-closing ventilation latency, the conventional treatment method requires approximately 2-4 weeks. The use of the hydrogen peroxide dry fog sterilizer is only applicable to the sterilization category, but the problem of nucleic acid aerosol pollution cannot be solved, and the accurate dosage of a PCR laboratory cannot be accurately measured, so that the method has no effect on the nucleic acid aerosol pollution.
Disclosure of Invention
Therefore, the technical problem to be solved by the utility model is to provide a nucleic acid aerosol pollution removal instrument and a control method thereof, so as to overcome the defects that the prior art lacks a device for removing nucleic acid aerosol in a PCR laboratory, and the time for manually removing the nucleic acid aerosol is long and the efficiency is low.
In order to solve the problems, the utility model provides a nucleic acid aerosol pollution removal instrument, which comprises an outer shell, wherein an air inlet and an air outlet are formed in the outer shell, a liquid storage tank for storing a nucleic acid aerosol scavenger, a spray head, an air duct and a fan in the air duct are arranged in the outer shell, the fan can drive air flow of an external environment to flow into the air duct through the air inlet and flow out of the air outlet, the scavenger in the liquid storage tank can be pumped to the spray head to form dry fog to spray, and the sprayed dry fog is circularly conveyed to a space where the nucleic acid aerosol pollution removal instrument is located along with the air flow formed under the driving of the fan.
In some embodiments, the spray head is provided with two symmetrically arranged scavenger spray nozzles and an air flow nozzle arranged between the two scavenger spray nozzles, the spraying paths of the two symmetrically arranged scavenger spray nozzles and the spraying path of the air flow nozzle intersect at the same point, an air compressor is further arranged in the outer shell, and an air outlet of the air compressor is communicated with the air flow nozzle.
In some embodiments, a temperature control device is further provided adjacent to the air compressor, the temperature control device being used to cool the air compressor.
In some embodiments, the fan is a centrifugal fan, an air suction opening of the centrifugal fan is opposite to the air inlet, an air outlet of the centrifugal fan is opposite to the air outlet through the air duct, the air outlet is provided with two parallel sub-runners, outlets of the sub-runners respectively correspond to the air outlet, and the spray head is positioned at a cross collecting position of the two sub-runners and is positioned outside the sub-runners.
In some embodiments, a plasma generator is respectively arranged in each air outlet; and/or the upper cover of the spray head is provided with a protective cover.
In some embodiments, a pressing sensing device is arranged near the protective cover and can automatically sense whether the protective cover is opened or not, and when the protective cover is not opened, the pressing sensing device senses and sends a corresponding signal to a control system of the instrument.
In some embodiments, a purifying and adsorbing component is further arranged between the air inlet and the air suction inlet of the fan, and/or the outer shell comprises a front shell, a rear shell, a middle shell and a bottom shell, and universal wheels are arranged below the bottom shell; a touch screen is arranged on the middle shell, and a plasma air outlet cover plate is positioned above the middle shell and provided with an air outlet grid; the air inlet is independently arranged on a detachable door plate which is detachably connected with the rear shell.
In some embodiments, one side of the purification and adsorption component is an aerosol adsorption screen, the other side is a photocatalyst structure, and the photocatalyst structure is positioned on one side of the purification and adsorption component far away from the air inlet.
In some embodiments, the device further comprises a laser ranging component for detecting the volume of the space in which the nucleic acid aerosol contaminant removal instrument is located; and/or the liquid storage tank is communicated with the scavenger spray nozzle in parallel through a peristaltic pump, and an anti-blocking filter screen is arranged on a pipeline between the peristaltic pump and the liquid storage tank.
In some embodiments, the laser ranging components are infrared laser ranging sensors respectively arranged at the front, back, left and right and upper sides of the outer shell.
According to the nucleic acid aerosol pollution remover provided by the utility model, the scavenger is sprayed out by the spray head in the form of dry fog, the dry fog is circularly sent to each corner of a space (particularly a PCR laboratory) where the nucleic acid aerosol pollution remover is located along with air flow formed under the drive of the fan, and the scavenger is diffused in the external space in the form of dry fog, so that the scavenger is more micronized and equalized, the scavenger can be used for more fully capturing PCR aerosol in air, efficiently destroying conjugated double bonds of purine and pyrimidine bases of DNA, effectively removing amplification product pollution rich in G+C and short (< 300 bp), and further efficiently completing the purpose of removing nucleic acid pollution in the PCR laboratory.
Drawings
FIG. 1 is an exploded view of a nucleic acid aerosol contaminant removal device according to an embodiment of the present utility model;
FIG. 2 is a schematic view of the spray head of FIG. 1;
FIG. 3 is a schematic perspective view of a nucleic acid aerosol contaminant removal device according to an embodiment of the present utility model;
FIG. 4 is a schematic control flow diagram of a nucleic acid aerosol contaminant removal device in accordance with an embodiment of the present utility model.
The reference numerals are expressed as:
11. an air inlet; 12. an air outlet; 121. a plasma generator; 21. a liquid storage tank; 22. a spray head; 221. a scavenger spray nozzle; 222. an air flow nozzle; 223. a protective cover; 23. an air compressor; 231. a temperature control device; 232. an electronized module assembly; 3. an air duct; 4. a blower; 5. a purification adsorption assembly; 6. a laser ranging assembly; 101. a front shell; 1011. revolving door the method comprises the steps of carrying out a first treatment on the surface of the; 1012. a rear case; 1013. a middle shell; 1014. a bottom case; 1021. a rear shell bouncing switch; 1031. a spray head support assembly; 1032. a bottom shell supporting frame; 1033. a liquid storage tank support; 104. an air quality sensor; 105. a liquid storage tank detector; 106. a device switch; 107. a universal wheel; 108. a touch screen; 1091. a plasma air outlet cover plate; 1092. and a main frame.
Detailed Description
Referring to fig. 1 to 4, according to an embodiment of the present utility model, there is provided a nucleic acid aerosol pollution remover, including an outer housing, on which an air inlet 11 and an air outlet 12 are configured, a liquid storage tank 21 for storing a nucleic acid aerosol scavenger, a spray head 22, an air duct 3, and a fan 4 in the air duct 3 are disposed in the outer housing, the fan 4 can drive an air flow of an external environment to flow into the air duct 3 through the air inlet 11 and flow out of the air outlet 12, the scavenger in the liquid storage tank 21 can be pumped to the spray head 22 to form a dry fog to spray, the sprayed dry fog can flow along with the air flow flowing out of the air outlet 12, and the nucleic acid aerosol scavenger is specifically a corresponding liquid configured according to a preset proportion. In this technical scheme, the scavenger is sprayed by the spray head 22 in the form of dry fog, and this part of dry fog is sent to each corner of the space where the nucleic acid aerosol pollution scavenger is located (specifically, PCR laboratory) along with the air flow formed by the fan 4, because the scavenger is diffused in the external space in the form of dry fog, the scavenger is more micronized and equalized, so the scavenger can more fully capture PCR aerosol in the air, efficiently destroy the conjugated double bonds of purine and pyrimidine bases of DNA, and can effectively remove amplification product pollution rich in g+c and short (< 300 bp), thereby the purpose of nucleic acid pollution removal in PCR laboratory can be accomplished with high efficiency. In addition, the spray head 22 in the present application sprays the scavenger in the form of dry mist, and the dry mist does not form condensation on the object surface, thereby reducing the corrosion thereof to the object surface. The spray head 22 may be a commercially available dry mist nozzle.
In a preferred embodiment, as shown in fig. 2, the spray head 22 is provided with two symmetrically arranged scavenger spray nozzles 221 and an air flow nozzle 222 positioned between the two scavenger spray nozzles 221, the spraying paths of the two symmetrically arranged scavenger spray nozzles 221 and the spraying paths of the air flow nozzle 222 intersect at the same point, an air compressor 23 is further arranged in the outer shell and is used for forming high-speed air flow, and the air outlet of the air compressor 23 is communicated with the air flow nozzle 222. The spray head 22 of the present utility model allows for finer particle sizes, and the addition of the gasifying nature of the special scavenger itself allows for better removal of nucleic acid contamination.
In some embodiments, a temperature control device 231 is further disposed adjacent to the air compressor 23, the temperature control device 231 is used for cooling the air compressor 23, and the occurrence of shutdown phenomenon caused by excessively high temperature rise caused by long-time operation of the air compressor 23 is effectively prevented, specifically, the temperature control device 231 can be a plurality of cooling fans, the plurality of cooling fans are specifically disposed around the air compressor 23, so that the cooling of the air compressor 23 can be realized through the driving of air flow, and of course, the cooling can be formed by the electronic module assembly 232 adjacent to the air compressor 23, so that the failure or damage caused by overheat is prevented, and the problem that the electronic components are damaged due to excessively high temperature in the casing is effectively prevented by the 3 cooling fans.
In a specific embodiment, the fan 4 is a centrifugal fan, the air inlet 11 and the air outlet 11 of the centrifugal fan are opposite, the air outlet 12 of the centrifugal fan is opposite to the air outlet 12 through the air duct 3, the air outlet 12 is provided with two parallel sub-channels, the air duct 3 is approximately Y-shaped in appearance, the outlets of the sub-channels respectively correspond to the air outlet 12, the spray head 22 is positioned at the cross-collecting position of the two sub-channels and is positioned at the outer side of the sub-channels, and thus, dry fog formed by spraying the spray head 22 is wrapped by air flows flowing out of the two sub-channels, so that the scavenger can flow and spread to all corners in space along with the air flows, the contact area between the scavenger and the aerosol is greatly improved, the thorough removal of the aerosol is facilitated, and the removal efficiency is further improved. In a specific embodiment, the spray head 22 is covered with a protective cover 223 for protecting the spray head 22, meanwhile, a pressing sensing device is disposed near the protective cover 223 to automatically sense whether the protective cover 223 is opened, the aforementioned pressing sensing device can be specifically an infrared sensing switch or the like, when the protective cover 223 is not opened, the pressing sensing device senses and sends a corresponding signal to a control system of the instrument, the peristaltic pump and the air compressor cannot be controlled to operate, and corresponding prompt information is displayed on the touch screen.
In a preferred embodiment, a purifying and adsorbing assembly 5 is further arranged between the air inlet 11 and the air inlet of the fan 4, so that air in a laboratory can be further purified and adsorbed. Specifically, one side of the purification adsorption component 5 is an aerosol adsorption screen, and the other side is a photocatalyst structure, namely, the purification adsorption component 5 integrates the aerosol adsorption screen and the photocatalyst structure, so that the number of components is reduced, and the assembly and subsequent maintenance of the cleaning instrument can be facilitated. The aerosol adsorption screen adopts the principle of an aerosol adsorber on the market to form physical adsorption of nucleic acid aerosol with the particle size of more than 0.3 microns, the photocatalyst structure generates photocatalytic reaction under the illumination of an LED ultraviolet lamp component (particularly 2 groups can be adopted), hydroxyl free radicals with super-strong oxidizing capacity are generated, the PCR aerosol is further degraded, and meanwhile, under the adsorption action of the aerosol adsorption screen, the cleaning effect of the scavenger is combined, so that the nucleic acid aerosol pollution is cooperatively cleaned from multiple dimensions by the scavenger, the synergistic effect is generated, and the effect is better.
The photocatalyst structure is positioned on one side of the purification and adsorption assembly 5 far away from the air inlet 11, so that the air flow in the external space firstly eliminates the nucleic acid aerosol with the particle size of more than 0.3 microns mixed in the air flow through the physical adsorption of the aerosol adsorption screen, the photocatalyst structure further effectively eliminates the nucleic acid aerosol with the particle size of less, and the special explanation is needed that the photocatalyst structure is arranged on the inner side of the purification and adsorption assembly 5, namely far away from the air inlet 11, so that the potential injury of ultraviolet rays to people can be avoided. In a specific embodiment, the photocatalyst has a high temperature resistance of 450 ℃ or more and a photocatalyst content of 230g/m 2 . The two-in-one structure of the aerosol adsorption screen and the photocatalyst structure is integrated on one component, is flatter and is positioned at the position of the detachable bouncing switch of the equipment rear shell, and relates to the two-in-one structure of replacing the aerosol adsorption screen and the photocatalyst once a half year to a year, so that laboratory staff can replace the aerosol adsorption screen and the photocatalyst very easily without any additional auxiliary tool.
As a preferred scheme, each air outlet 12 is respectively provided with a plasma generator 121, and the positive ions and negative ions generated by the plasma generators 121 simultaneously generate huge energy release at the moment of neutralizing positive charges and negative charges in the air, so that harmful pathogens, harmful gases and residues are effectively removed.
In some embodiments, the nucleic acid aerosol pollution removal instrument is further provided with a laser ranging component 6, which is used for detecting the volume of the space where the nucleic acid aerosol pollution removal instrument is located, the laser ranging component 6 can be specifically an infrared laser ranging sensor, and the number of the laser ranging component 6 can be specifically 5, and the laser ranging sensor can be respectively arranged at the front, the back, the left, the right and the upper sides of the outer shell, so that the space volume of a PCR laboratory is automatically calculated without manual measurement or external auxiliary equipment, and the required liquid adding amount of each experiment is automatically calculated. In a specific embodiment, the infrared laser ranging sensor adopts IIC (inter-integrated circuit) and UART (universal asynchronous receiver/transmitter) serial port communication formats, the LED light source is 850nm, the resolution is 1cm, the measuring range can reach 4000 cubes, and the updating frequency is more than or equal to 195Hz. The general PCR laboratory partitions are between 30-200 cubic, the automatic measurement is completely satisfied, and the amount of scavenger adding liquid required by each laboratory partition is automatically displayed through a display screen by an algorithm. Prevent laboratory staff maloperation, perhaps the staff estimates the inaccurate condition of laboratory space volume, guarantees the use of experiment and clears away the accurate of dosage, practices thrift laboratory consumptive material use amount, and the result of guarantee clearance is more effective.
The liquid storage tank 21 is communicated with the scavenger spray nozzle 221 in parallel through a peristaltic pump (not marked in the figure), and an anti-blocking filter screen is arranged on a pipeline between the peristaltic pump and the liquid storage tank 21 to prevent the scavenger from blocking when flowing to the scavenger spray nozzle 221 through the peristaltic pump. After the experiment operation is finished, only the flushing liquid storage tank 21 is required to be cleaned, so that the operation personnel in the laboratory can use the flushing liquid storage tank conveniently.
According to an embodiment of the present utility model, there is also provided a control method of a nucleic acid aerosol contamination removal apparatus for controlling the above-mentioned nucleic acid aerosol contamination removal apparatus, the control method including the steps of:
acquiring an operation mode of a nucleic acid aerosol pollution removal instrument;
when the operation mode is the aerosol removal mode, controlling the peristaltic pump, the air compressor 23 and the blower 4 to operate, stopping the operation for a second preset time (generally 30 minutes) after the operation for the first preset time, and then restarting the blower 4 and controlling the photocatalyst structure and the plasma generator 121 to operate;
when the operation mode is the adsorption purification mode, the photocatalyst structure, the plasma generator 121 and the blower 4 are controlled to operate, and the two operation modes can be selected by an operator according to actual needs.
In a preferred embodiment, before the aerosol cleaning mode is operated, the volume information of the space where the nucleic acid aerosol pollution cleaning instrument is located is obtained by a laser ranging assembly or a manually set manner, and the liquid adding amount of the acid aerosol cleaning agent in the liquid storage tank 21 is calculated according to the obtained volume information.
Referring to fig. 3, the purging apparatus of the present utility model has an aerosol purging mode and an adsorption purging mode, with the particular mode of operation being selected by the operator. When an operator selects an aerosol cleaning mode, the cleaning instrument operates the cleaning mode, in the cleaning mode, the equipment is only required to be pushed to any position of a laboratory, automatic ranging is carried out on the equipment (no obstacle is required to be arranged at the corresponding position of a ranging component in each direction of the instrument), the space volume of a PCR laboratory is automatically calculated, the required liquid adding amount of each experiment is automatically calculated, a cleaning agent in a liquid storage tank is subjected to dry atomization through an air compressor in the equipment box body, a peristaltic pump works, the cleaning agent is subjected to dry atomization through a spray head 22, then stationary reaction is carried out for 30 minutes, then a fan of the equipment is automatically started, an aerosol adsorption screen is automatically started through an air inlet, a photocatalyst reaction is automatically started, and the cleaning whole process is completed through an air outlet plasma generator, so that the nucleic acid aerosol pollution cleaning work is achieved. When an operator selects an adsorption purification mode, a fan of the equipment is automatically started, an aerosol adsorption screen and a photocatalyst reaction are automatically started through an air inlet, and the aerosol adsorption screen and the photocatalyst reaction are discharged through an air outlet plasma generator, so that the physical and chemical removal whole process is completed, and the work of removing the nucleic acid aerosol pollution is achieved. The aerosol cleaning mode and the adsorption purification mode are completely independent, do not influence each other, and can be freely switched according to laboratory conditions. The independent control operation is simpler, and an electric control unit for switching the operation modes is not needed.
The technical scheme of the utility model is further described below with reference to fig. 1.
As shown in fig. 1, the nucleic acid aerosol contamination remover comprises an outer housing comprising a front housing 101, a rear housing 1012, a middle housing 1013, a bottom housing 1014, and four universal wheels 107 under the bottom housing 1014 for convenient movement of the device. The top of the outer shell is provided with a high definition touch screen 108 (on the middle shell 1013), a spray head supporting assembly 1031 and a spray head 22 on the supporting assembly, a protective cover 223 is covered on the spray head 22, a plasma air outlet cover plate 1091 is positioned above the middle shell 1013 and provided with an air outlet grille, and the side surface of the middle shell 1013 is provided with an equipment switch 106. The high definition touch screen 108 may be used for switching settings of a nucleic acid aerosol removal mode and an adsorption purge mode. The protective cover 223 is provided with a pressing sensing device which can automatically sense whether the protective cover 223 is opened or not, thereby protecting the spray head 22, and the pressing sensing device has a prompt and early warning function. The spray head 22 has a rotating function, and is convenient to detach, clean and replace. The air inlet 11 is separately located on a detachable door panel, which is detachably connected with the rear shell 1012, and in a specific embodiment, the detachable connection between the door panel and the rear shell 1012 can be easily realized through the rear shell bouncing switch 1021, so that the maintenance and replacement of the purification adsorption assembly 5 are facilitated.
Laser ranging assembly 6: the infrared laser ranging device is used for automatically measuring and calculating the volume of a room where equipment is located, and five infrared laser ranging sensors are respectively arranged on the front side, the rear side, the left side, the right side and the upper side of the equipment shell. The measured room volume is automatically converted into the required liquid adding amount through an algorithm. Liquid storage tank 21: the tank support 1033 is used for fixing the tank position, and a tank detector 105 is arranged below the tank support 1033 for detecting the weight of the tank 21. The front housing 101 of the device is provided with a rotatable door 1011 and attached with a magnet means to facilitate the extraction and replacement of the reservoir 21 into the housing. A filter screen is arranged at the water outlet of the liquid storage tank 21 to prevent the scavenger from blocking when flowing to the spray head 22 through the peristaltic pump.
The compressed air of the air compressor 23 flows at a high speed, and the liquid is atomized by the spray heads 22 which are arranged at two dry fog nozzles, so that 3 groups of cooling fans are arranged below the air compressor 23 for preventing the air compressor 23 from working for a long time, and the temperature is reduced when the air compressor 23 works.
It can be understood that the main frame 1092 for assembling the internal components is provided inside the outer housing, and accordingly, the air duct 3 and the fan 4 are assembled above and in the middle area of the main frame 1092, the bottom shell support 1032 is positioned in the lower area of the main frame 1092, and the air compressor 23, the temperature control device 231 and the electronic module assembly 232 are assembled on the bottom shell support 1032. The scavenger also has an air quality sensor 104.
The fan 4 is used for extracting air in a laboratory room, the purification and adsorption assembly 5 integrating photocatalyst and aerosol adsorption screen is arranged on the outer side of the fan 4, namely, the photocatalyst is arranged on one side of the assembly, titanium dioxide generates photocatalytic reaction under the illumination of the LED ultraviolet lamp assembly to generate hydroxyl free radicals with super-strong oxidizing capacity, and the aerosol adsorption screen is arranged on one side of the air inlet. The inlet air passes through the purification adsorption assembly 5 and enters the inlet air channel, air can only enter the shell through the inlet air channel, the inlet air channel and the outlet air channel are arranged on the channel assembly, the air flows unidirectionally and is discharged from the air outlet cover plate after being processed by the plasma generator 121, and a fence opening is arranged on the air outlet plate, so that air is conveniently discharged and the air outlet channel is protected. The purge adsorption module 5 is replaced periodically as needed.
When the embodiment is used, the special scavenger is configured, the scavenger is poured into the liquid storage tank 21, the equipment is placed at any non-shielding position in a laboratory, after the main interface is lightly touched to clear, space measurement is clicked, the volume of a room in the PCR laboratory is automatically measured, a determination key is clicked, and the room volume can be manually set. After the space measurement interface clicks the return button or after the room volume is manually input, the system program automatically calculates the amount of the nucleic acid scavenger required, and before the system program runs, the amount of the nucleic acid scavenger required to be added must be ensured to be more than the amount of the actual nucleic acid scavenger required. Then clicking the 'clear' button, starting the device immediately after the system counts down for 10 seconds, and starting the device when the device enters a nucleic acid aerosol clearing mode state, wherein a green light of the working state around the device flashes, so that the device is in a normal working state. In the state of the cleaning mode, the system can automatically operate the processes of the cleaning agent spraying stage, the standing action reaction stage, the adsorption screen physical adsorption stage and the photocatalyst reaction stage without manual staged re-operation. In the main menu of the screen, click the "set" function key on the right side of the screen, i.e. enter the setup interface. Defaults to the factory setting of 10mL/min flow, 30min standing time, 30min recovery time and 8mL/m clearing concentration 3 。
When the adsorption purification device is used, the adsorption purification function is started by clicking the adsorption purification at the lower left part of the screen, the equipment work indicator lamp is in a blue flashing state, and after the adsorption purification work is finished, the equipment work indicator lamp is in a blue normally-on state. The photocatalyst function and the adsorption function can coexist with human-machine, and the action duration can be modified in the program editing interface. The adsorption and purification work is completed, and the equipment work indicator lamp is in a blue normally-on state.
The automated purge mode and the adsorption mode may be freely switched by the high definition touch screen 108.
It will be readily appreciated by those skilled in the art that the above advantageous ways can be freely combined and superimposed without conflict.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model. The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that it will be apparent to those skilled in the art that modifications and variations can be made without departing from the technical principles of the present utility model, and these modifications and variations should also be regarded as the scope of the utility model.
Claims (10)
1. The utility model provides a nucleic acid aerosol pollutes and clears away appearance, its characterized in that, includes the shell body, be constructed with air intake (11) and air outlet (12) on the shell body, be equipped with in the shell body and be used for storing reservoir (21), atomising head (22), wind channel (3), be in fan (4) in wind channel (3), fan (4) can drive the air current of external environment via air intake (11) inflow wind channel (3) are followed air outlet (12) outflow, the scavenger in reservoir (21) can be pumped to atomising head (22) department forms the dry fog blowout, the spun dry fog follows the air current circulation that forms under fan (4) drive and send to the space that nucleic acid aerosol pollutes and clears away appearance place.
2. The nucleic acid aerosol contamination removal apparatus as set forth in claim 1, wherein the spray head (22) has two symmetrically disposed scavenger spray nozzles (221) and an air flow nozzle (222) disposed between the two scavenger spray nozzles (221), the discharge paths of the two symmetrically disposed scavenger spray nozzles (221) intersect with the discharge path of the air flow nozzle (222) at the same point, an air compressor (23) is further provided in the outer case, and an air outlet of the air compressor (23) communicates with the air flow nozzle (222).
3. The nucleic acid aerosol contamination removal apparatus as set forth in claim 2, further comprising a temperature control device (231) adjacent to the air compressor (23), the temperature control device (231) being configured to cool the air compressor (23).
4. The nucleic acid aerosol pollution removal instrument according to claim 2, wherein the fan (4) is a centrifugal fan, an air suction inlet of the centrifugal fan is opposite to the air inlet (11), an air outlet of the centrifugal fan is opposite to the air outlet (12) through the air duct (3), the air outlet (12) is provided with two parallel sub-runners, outlets of the sub-runners respectively correspond to the air outlet (12), and the spray head (22) is positioned at a cross-collecting position of the two sub-runners and is positioned outside the sub-runners.
5. The nucleic acid aerosol contamination removal apparatus as set forth in claim 4, wherein each of the air outlets (12) is provided with a plasma generator (121), respectively; and/or the upper cover of the spray head (22) is provided with a protective cover (223).
6. The nucleic acid aerosol contamination removal apparatus of claim 5, wherein a push sensing device is disposed adjacent to the protective cover (223) to automatically sense whether the protective cover (223) is opened, and wherein the push sensing device senses and transmits a corresponding signal to a control system of the apparatus when the protective cover (223) is not opened.
7. The nucleic acid aerosol contamination removal instrument according to claim 1, wherein a purification adsorption assembly (5) is further arranged between the air inlet (11) and the air suction inlet of the fan (4), and/or the outer housing comprises a front housing (101), a rear housing (1012), a middle housing (1013) and a bottom housing (1014), and a universal wheel (107) is arranged below the bottom housing (1014); a touch screen (108) is arranged on the middle shell (1013), and a plasma air outlet cover plate (1091) is positioned above the middle shell (1013) and is provided with an air outlet grid; the air inlet (11) is independently arranged on a detachable door plate which is detachably connected with the rear shell (1012).
8. The nucleic acid aerosol contamination removal apparatus of claim 7, wherein one side of the purification adsorption assembly (5) is an aerosol adsorption screen, the other side is a photocatalyst structure, and the photocatalyst structure is located at a side of the purification adsorption assembly (5) away from the air inlet (11).
9. The nucleic acid aerosol contamination removal instrument of claim 2, further comprising a laser ranging assembly (6) for detecting a volume of a space in which the nucleic acid aerosol contamination removal instrument is located; and/or the liquid storage tank (21) is communicated with the scavenger spray nozzle (221) in parallel through a peristaltic pump, and an anti-blocking filter screen is arranged on a pipeline between the peristaltic pump and the liquid storage tank (21).
10. The nucleic acid aerosol contamination removal instrument according to claim 9, wherein the laser ranging component (6) is an infrared laser ranging sensor, which is disposed on the front, rear, left and right sides and upper side of the outer case, respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223043959.2U CN219333612U (en) | 2022-11-16 | 2022-11-16 | Instrument for removing pollution of nucleic acid aerosol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223043959.2U CN219333612U (en) | 2022-11-16 | 2022-11-16 | Instrument for removing pollution of nucleic acid aerosol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219333612U true CN219333612U (en) | 2023-07-14 |
Family
ID=87103015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223043959.2U Active CN219333612U (en) | 2022-11-16 | 2022-11-16 | Instrument for removing pollution of nucleic acid aerosol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219333612U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118237329A (en) * | 2024-05-30 | 2024-06-25 | 济南国际旅行卫生保健中心(济南海关口岸门诊部) | Nucleic acid experiment aerosol pollutes belt cleaning device |
-
2022
- 2022-11-16 CN CN202223043959.2U patent/CN219333612U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118237329A (en) * | 2024-05-30 | 2024-06-25 | 济南国际旅行卫生保健中心(济南海关口岸门诊部) | Nucleic acid experiment aerosol pollutes belt cleaning device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN219333612U (en) | Instrument for removing pollution of nucleic acid aerosol | |
| KR102203201B1 (en) | An air cleaning system and a method controlling the same | |
| KR100819077B1 (en) | Method for displaying filter exchange time of air cleaner | |
| JP6491767B2 (en) | Air cleaner | |
| CN110585464B (en) | Sterilization equipment and sterilization method for biochemical laboratory | |
| JP6202219B2 (en) | Air cleaner | |
| CN108305699B (en) | Intelligent multifunctional radioactivity-removing instrument | |
| CN105651944A (en) | Animal aerosol exposure experiment system | |
| JP4761497B2 (en) | isolator | |
| CN112945837A (en) | Real-time monitoring device for bioaerosol | |
| CN114088477B (en) | Wet wall cyclone type air aerosol sampler | |
| KR20190060496A (en) | Fume hood | |
| CN208288032U (en) | Gas treatment Biohazard Safety Equipment | |
| CN115738551A (en) | Nucleic acid aerosol pollution eliminating instrument and control method thereof | |
| CN211706804U (en) | Novel biological safety cabinet | |
| CN210932918U (en) | Disinfection equipment for biochemical laboratory | |
| CN112246290A (en) | Air purification type integrated experiment cabinet and working method thereof | |
| KR20210024817A (en) | Air purifier suitable for mandatory ventilation | |
| CN212417979U (en) | Integrated laboratory cabinet of air-purifying formula | |
| KR200344799Y1 (en) | Filtering Ductless Fume Hood Using the Zeolite Filter | |
| JP6435171B2 (en) | Air conditioner | |
| CN213102244U (en) | Nucleic acid extraction instrument, filtering device, biological safety cabinet and superclean bench | |
| CN216550407U (en) | Anti-pollution nucleic acid check out test set | |
| CN214132229U (en) | Vertical high-capacity aerosol purification centrifuge | |
| CN219072554U (en) | Air purification and drying device for cell laboratory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |