CN115738551A - Nucleic acid aerosol pollution eliminating instrument and control method thereof - Google Patents

Abstract

The invention provides a nucleic acid aerosol pollution remover and a control method thereof, wherein the remover comprises an outer shell, an air inlet and an air outlet are formed on the outer shell, a liquid storage tank for storing a nucleic acid aerosol remover, a spray head, an air duct and a fan positioned in the air duct are arranged in the outer shell, the fan can drive airflow of an external environment to flow into the air duct through the air inlet and flow out from the air outlet, a remover in the liquid storage tank can be pumped to the spray head to form dry mist to be sprayed out, and the sprayed dry mist can flow along with the airflow flowing out from the air outlet. The dry fog is circularly sent to each corner of the space where the nucleic acid aerosol pollution remover is positioned along with the airflow formed under the driving of the fan, so that the remover is more micronized and equalized, the remover can more fully capture PCR aerosol in the air, and the conjugated double bonds of purine and pyrimidine base of DNA are efficiently destroyed, thereby efficiently finishing the aim of removing nucleic acid pollution in a PCR laboratory.

Description

Nucleic acid aerosol pollution eliminating instrument and control method thereof

Technical Field

The invention belongs to the field of design of a nucleic acid aerosol pollution eliminating device, and particularly relates to a nucleic acid aerosol pollution eliminating instrument and a control method thereof.

Background

PCR detection is widely used due to its advantages of high sensitivity, rapidness, convenient operation, etc., but due to its extremely high sensitivity, trace contamination sources may cause false positive of the detection result. When PCR experiments are carried out, the particle size of nucleic acid aerosol suspended in a laboratory is generally 0.001-1000 μm, the reaction tube is shaken violently during operation, and nucleic acid aerosol can be formed during uncovering, sample sucking and repeated sample sucking of a pipettor, the nucleic acid aerosol widely exists on a laboratory table top, a biological safety cabinet top, the pipettor, a door handle, a PCR instrument, a centrifuge and the like along with the strength and the time of the experiments, and the copy quantity of PCR amplification products is large (generally 10) ¹³ copy/mL), well above the limit of PCR detection-several copies, so that a false positive can be formed with minimal contamination of the PCR product.

PCR contamination is generally classified into four categories: 1. cross contamination among samples, mainly in the sampling process, due to the cross contamination among sampling tools; or contamination among samples caused by improper use of pipettors, centrifuge tubes and the like in the process of nucleic acid extraction; contamination of PCR reagents, mainly during the PCR reagent preparation process, due to contamination of pipettors, containers, negative controls and other reagents by nucleic acid templates or positive controls; 3. the pollution of the cloned plasmid often uses positive reference substances in laboratory operation, most of the positive reference substances are made of certain cloned plasmids, and the cloned plasmids have high concentration in unit volume and are extremely easy to cause pollution when being improperly used; contamination of PCR amplification products, which is the most prominent, most common and most troublesome contamination problem in PCR reactions. Because the copy quantity of the PCR product is large and is far higher than the limit of detecting several copies by PCR, a trace amount of PCR product pollution can cause false positive results.

The most difficult to eliminate in PCR laboratory nucleic acid contamination is aerosol contamination, especially of amplification products. Contamination occurs primarily during the amplification and product analysis stages of PCR because of the large copy number of PCR products (typically 10) ¹³ copy/mL), the amplification product forms an aerosol when the liquid surface is rubbed with airThe glue, so shaking the reaction tube more vigorously, sucking the sample when the cover is opened, and repeatedly sucking the sample can form aerosol pollution. The splashed amplification products form aerosol floating in the air, adhere to the surface of an object and the body of a detection person, and pollute the whole laboratory along with the flow of the person and materials. If such contamination requires the laboratory to be left idle for many days and active and effective measures are taken, the situation will improve.

At present, the experiment needs to be suspended in the first step when the experiment meets pollution, and the influence of false positive on the past detection result is evaluated. Thoroughly clean and clear up the laboratory, change relevant reagent consumptive material as far as, clean and the sour processing of denucleating the instrument and equipment, operating personnel bathes and trades the clothes. The traditional processing method comprises the following steps: chemical treatments, which typically present carcinogenicity, interfere with detection of amplification products, are inefficient in controlling G + C rich and short amplification products, are not stable, and the like. Ultraviolet lamp irradiation method: it is only effective for long fragments of more than 300bp, and has little effect on short fragments. Also with windowed ventilation latency, conventional processing methods may require a duration of approximately 2 weeks to 4 weeks. The hydrogen peroxide dry fog sterilizer is only correspondingly applied to the sterilization scope, and cannot solve the problem of nucleic acid aerosol pollution, cannot accurately measure the accurate dosage of a PCR laboratory, and has no effect on nucleic acid aerosol pollution.

Disclosure of Invention

Therefore, the technical problem to be solved by the present invention is to provide a nucleic acid aerosol contamination remover and a control method thereof, so as to overcome the defects that the prior art lacks a nucleic acid aerosol removing device for a PCR laboratory, and the manual nucleic acid aerosol removing method is time-consuming, long and inefficient.

In order to solve the above problems, the present invention provides a nucleic acid aerosol pollution removal apparatus, which includes an outer casing, an air inlet and an air outlet are configured on the outer casing, a liquid storage tank for storing a nucleic acid aerosol scavenger, a spray head, an air duct, and a blower in the air duct are arranged in the outer casing, the blower can drive the air flow of the external environment to flow into the air duct through the air inlet and flow out from the air outlet, a scavenger in the liquid storage tank can be pumped to the spray head to form a dry mist to be sprayed, and the sprayed dry mist can flow along with the air flow flowing out from the air outlet.

In some embodiments, the spray head is provided with two symmetrically arranged scavenger spray nozzles and an air flow nozzle located between the two scavenger spray nozzles, the spraying paths of the two symmetrically arranged scavenger spray nozzles intersect at the same point with the spraying path of the air flow nozzle, and an air compressor is further arranged in the outer shell, and an air outlet of the air compressor is communicated with the air flow nozzle.

In some embodiments, a temperature control device is further provided adjacent to the air compressor for cooling the air compressor.

In some embodiments, the fan is centrifugal fan, centrifugal fan's inlet scoop with the air intake sets up relatively, centrifugal fan's air exit passes through the wind channel with the air outlet sets up relatively, the air outlet has two, the wind channel has two parallel sub-runners, and the export of each sub-runner corresponds one respectively the air outlet, the atomising head is in two the alternately position of converging of sub-runner and is in the outside of sub-runner.

In some embodiments, a plasma generator is disposed in each of the air outlets; and/or a protective cover is covered on the spray head.

In some embodiments, a purification and adsorption assembly is further disposed between the air inlet and the air suction opening of the fan.

In some embodiments, one side of the purification and adsorption component is an aerosol adsorption screen, the other side of the purification and adsorption component is a photocatalyst structure, and the photocatalyst structure is located on one side of the purification and adsorption component away from the air inlet.

In some embodiments, the nucleic acid aerosol pollution remover further comprises a laser ranging component for detecting the volume of the space where the nucleic acid aerosol pollution remover is located; and/or the liquid storage tank is communicated with the scavenger spray nozzle in parallel through a peristaltic pump, and an anti-blocking filter screen is arranged on a pipeline between the peristaltic pump and the liquid storage tank.

The invention also provides a control method of the nucleic acid aerosol pollution eliminating instrument, which is used for controlling the nucleic acid aerosol pollution eliminating instrument and comprises the following steps:

obtaining the operation mode of the nucleic acid aerosol pollution removing instrument;

when the operation mode is the aerosol removal mode, the peristaltic pump, the air compressor and the fan are controlled to operate, the operation is stopped for a second preset time after the operation is carried out for a first preset time, and then the fan is started again and the photocatalyst structure and the plasma generator are controlled to operate;

and when the operation mode is the adsorption purification mode, controlling the photocatalyst structure, the plasma generator and the fan to operate.

In some embodiments, before the aerosol cleaning mode is operated, volume information of a space where the nucleic acid aerosol pollution remover is located is obtained, and the amount of liquid adding of the nucleic acid aerosol remover in the liquid storage tank is calculated according to the obtained volume information.

According to the nucleic acid aerosol pollution removing instrument and the control method thereof provided by the invention, the remover is sprayed out by the spray head in the form of dry fog, the dry fog is circularly sent to each corner of a space (specifically a PCR laboratory) where the nucleic acid aerosol pollution removing instrument is located along with the air flow formed under the drive of the fan, and the remover is dispersed in the external space in the form of the dry fog, so that the remover can be more micronized and equalized, the PCR aerosol in the air can be more fully captured by the remover, the conjugated double bonds of purine and pyrimidine base of DNA can be efficiently destroyed, the G + C-rich and short (< 300 bp) amplification product pollution can be effectively removed, and the purpose of removing the nucleic acid pollution in the PCR laboratory can be efficiently completed.

Drawings

FIG. 1 is an exploded view of a nucleic acid aerosol contamination remover according to an embodiment of the present invention;

fig. 2 is a schematic view of the spray head of fig. 1;

FIG. 3 is a schematic perspective view of a nucleic acid aerosol contamination remover according to an embodiment of the present invention;

FIG. 4 is a control flow chart of the nucleic acid aerosol contamination remover according to the embodiment of the present invention.

The reference numbers are given as:

11. an air inlet; 12. an air outlet; 121. a plasma generator; 21. a liquid storage tank; 22. a spray head; 221. a scavenger spray nozzle; 222. an air flow nozzle; 223. a protective cover; 23. an air compressor; 231. a temperature control device; 232. an electronized module assembly; 3. an air duct; 4. a fan; 5. a purification and adsorption component; 6. a laser ranging assembly; 101. a front housing; 1011. a revolving door; 1012. a rear housing; 1013. a middle shell; 1014. a bottom case; 1021. a rear shell bounce switch; 1031. a sprayhead support assembly; 1032. a bottom shell support frame; 1033. a reservoir support member; 104. an air quality sensor; 105. a reservoir detector; 106. a device switch; 107. a universal wheel; 108. a touch screen; 1091. a plasma air outlet cover plate; 1092. a main frame.

Detailed Description

Referring to fig. 1 to 4 in combination, according to an embodiment of the present invention, there is provided a nucleic acid aerosol pollution removing apparatus, which includes an outer casing, an air inlet 11 and an air outlet 12 are formed on the outer casing, a liquid storage tank 21 for storing a nucleic acid aerosol scavenger, a spray head 22, an air duct 3, and a fan 4 in the air duct 3 are arranged in the outer casing, the fan 4 can drive an air flow of an external environment to flow into the air duct 3 through the air inlet 11 and flow out from the air outlet 12, the scavenger in the liquid storage tank 21 can be pumped to the spray head 22 to form a dry mist spray, and the sprayed dry mist can flow along with the air flow flowing out from the air outlet 12, and the nucleic acid aerosol scavenger is specifically a corresponding liquid configured according to a preset ratio. In the technical scheme, the scavenging agent is sprayed out in a form of dry fog by the spray head 22, the dry fog is circularly sent to each corner of a space (specifically a PCR laboratory) where the nucleic acid aerosol pollution scavenging instrument is located along with the air flow formed under the driving of the fan 4, and the scavenging agent is more micronized and equalized due to the fact that the scavenging agent is diffused in the external space in the form of the dry fog, so that the scavenging agent can more fully capture PCR aerosol in the air, efficiently destroy conjugated double bonds of purine and pyrimidine base of DNA, and effectively scavenge G + C-rich and short (< 300 bp) amplification product pollution, thereby efficiently completing the purpose of scavenging the nucleic acid pollution of the PCR laboratory. In addition, in the present application, the spray head 22 sprays the scavenger in the form of dry mist, and the dry mist state does not form condensation on the surface of the object, thereby reducing corrosion of the object surface. The spray head 22 may be a commercially available dry mist nozzle.

In a preferred embodiment, as shown in fig. 2, the spray head 22 has two symmetrically arranged scavenger spray nozzles 221 and an air flow nozzle 222 located between the two scavenger spray nozzles 221, the spray paths of the two symmetrically arranged scavenger spray nozzles 221 intersect at the same point as the spray path of the air flow nozzle 222, an air compressor 23 is further disposed in the outer housing for forming a high-speed air flow, and the air outlet of the air compressor 23 is communicated with the air flow nozzle 222. The spray head 22 of the invention has smaller particle size, and the gasification property of the special scavenging agent is added, so that the effect of scavenging nucleic acid pollution is better.

In some embodiments, a temperature control device 231 is further provided near the air compressor 23, the temperature control device 231 is used for cooling the air compressor 23, and the shutdown phenomenon caused by the excessively high temperature rise of the long-time operation of the air compressor 23 is effectively prevented, specifically, the aforementioned temperature control device 231 can be a plurality of heat dissipation fans which are specifically surrounded by the air compressor 23, so that the heat dissipation cooling of the air compressor 23 can be realized through the driving of the air flow, and certainly, the electronic module assembly 232 of the air compressor 23 which is near to be arranged can be cooled simultaneously, thereby preventing the overheating and the occurrence of failure or damage, and the problem that the electronic component is damaged due to the excessively high temperature in the casing is effectively prevented by the 3 heat dissipation fans.

In a specific embodiment, the fan 4 is a centrifugal fan, an air suction port of the centrifugal fan is arranged opposite to the air inlet 11, an air outlet of the centrifugal fan is arranged opposite to the air outlet 12 through the air duct 3, the air outlet 12 has two air outlets 12, the air duct 3 has two parallel sub-flow passages, the air duct 3 is approximately Y-shaped in appearance, an outlet of each sub-flow passage corresponds to one air outlet 12, and the atomizing head 22 is located at a cross collection position of the two sub-flow passages and outside the sub-flow passages, so that dry mist formed by spraying of the atomizing head 22 is wrapped by air flows flowing out of the two sub-flow passages, so that the scavenger can diffuse to each corner in a space along with the air flow, the contact area between the scavenger and aerosol is greatly increased, complete removal of the aerosol is facilitated, and the removal efficiency is further improved. In a specific embodiment, a protective cover 223 is covered on the atomizing head 22 for protecting the atomizing head 22, and meanwhile, a press sensing device is configured near the protective cover 223 and can automatically sense whether to open the protective cover 223, the press sensing device may be specifically an infrared sensing switch or the like, when the protective cover 223 is not opened, the press sensing device senses and sends a corresponding signal to a control system of the apparatus, the peristaltic pump and the air compressor cannot be controlled to operate, and a corresponding prompt message is displayed on the touch screen.

In a preferred embodiment, a purification and adsorption assembly 5 is further arranged between the air inlet 11 and the air suction opening of the fan 4, so that air in the laboratory can be further purified and adsorbed. Particularly, purify one side of adsorption component 5 and be the aerosol adsorption screen, the opposite side is the photocatalyst structure, also purifies adsorption component 5 and has integrateed aerosol adsorption screen and photocatalyst structure to reduce the quantity of part, the equipment and subsequent maintenance that can be convenient for clear away the appearance. The aerosol adsorption screen adopts the principle of an aerosol adsorber on the market to form physical adsorption on nucleic acid aerosol with the particle size of more than 0.3 micrometer, the titanium dioxide generates a photocatalyst reaction under the illumination of an LED ultraviolet lamp assembly (specifically 2 groups can be adopted) by using a photocatalyst structure to generate hydroxyl radicals with super-strong oxidation capacity to further degrade PCR aerosol, and meanwhile, under the adsorption effect of the aerosol adsorption screen, the scavenger combines the scavenging effect of the scavenger in the foregoing, so that the scavenger cooperatively scavenges the nucleic acid aerosol pollution from multiple dimensions, generates a synergistic effect and has a better effect.

The photocatalyst structure is located the one side that purifies adsorption component 5 and keep away from air intake 11, so, the air current of exterior space clears away the nucleic acid aerosol more than the very few 0.3 micron particle size that mixes in it through the physical adsorption of aerosol adsorption screen earlier, and the photocatalyst structure then further realizes effectively clearing away to the nucleic acid aerosol of less particle size, and need to explain very much, set up the photocatalyst structure in the inboard that purifies adsorption component 5 and also keep away from air intake 11, can stop the potential injury emergence of ultraviolet ray to the people. In one embodiment, the photocatalyst has a high temperature resistance of 450 deg.C or higher and a photocatalyst content of 230g/m ² . The aerosol adsorption screen and the photocatalyst structure are integrated on one component, are more flattened and are positioned at the position of the removable bounce switch of the rear shell of the equipment, and the aerosol adsorption screen and the photocatalyst are replaced once a year to a year, so that laboratory personnel can replace the aerosol adsorption screen and the photocatalyst very easily without any additional auxiliary tool.

Preferably, each air outlet 12 is provided with a plasma generator 121, and positive ions and negative ions generated by the plasma generator 121 simultaneously neutralize positive and negative charges in the air to instantly release huge energy, so as to effectively remove harmful pathogens, harmful gases and residues.

In some embodiments, nucleic acid aerosol pollution elimination appearance still has laser range finding subassembly 6 for detect the volume in the space that nucleic acid aerosol pollution elimination appearance was located, laser range finding subassembly 6 specifically can be infrared laser range finding sensor, and it specifically can set up 5, sets up respectively all around and the upside of shell body, reaches and need not artifical measurement or external auxiliary assembly, and the space volume of automatic calculation PCR laboratory calculates the liquid feeding volume of the needs of experiment at every turn automatically. In a specific embodiment, the infrared laser ranging sensor adopts IIC and UART serial port communication format, the LED light source is 850nm, the resolution is 1cm, the measuring range can reach 4000 cubes, and the updating frequency is more than or equal to 195Hz. Generally, each partition of the PCR laboratory is between 30 and 200 cubes, and the automatic measurement is completely satisfied, and the required adding amount of the scavenging agent of each partition of the laboratory is automatically displayed through a display screen through an algorithm. Prevent laboratory staff maloperation, perhaps the staff estimates the inaccurate condition of laboratory space volume, guarantee the use of experiment and clear away the accurate of dosage, practice thrift laboratory consumptive material use amount, the result that the guarantee was clear away is more effective.

The liquid storage tank 21 is communicated with the scavenger spray nozzle 221 in parallel through a peristaltic pump (not marked in the figure), and an anti-blocking filter screen is arranged on a pipeline between the peristaltic pump and the liquid storage tank 21 to prevent the scavenger from being blocked when the scavenger flows to the scavenger spray nozzle 221 through the peristaltic pump. After the experiment operation is finished, only the liquid storage tank 21 needs to be cleaned and flushed, so that the laboratory operation personnel can use the liquid storage tank conveniently.

According to an embodiment of the present invention, there is also provided a control method for a nucleic acid aerosol contamination removal apparatus, for controlling the nucleic acid aerosol contamination removal apparatus, the control method including the steps of:

obtaining the operation mode of the nucleic acid aerosol pollution removing instrument;

when the operation mode is the aerosol removal mode, the peristaltic pump, the air compressor 23 and the fan 4 are controlled to operate, the operation is stopped for a second preset time (generally 30 minutes) after the operation is carried out for the first preset time, and then the fan 4 is started again and the photocatalyst structure and the plasma generator 121 are controlled to operate;

when the operation mode is the adsorption purification mode, the photocatalyst structure, the plasma generator 121 and the fan 4 are controlled to operate, and the two operation modes can be selected by an operator to operate according to actual needs.

In a preferred embodiment, before the aerosol removing mode is operated, the volume information of the space where the nucleic acid aerosol pollution remover is positioned is obtained through a laser ranging component or a manual setting mode, and the filling amount of the nucleic acid aerosol remover in the liquid storage tank 21 is calculated according to the obtained volume information.

Referring to fig. 3, the cleaning instrument of the present invention has an aerosol cleaning mode and an adsorption cleaning mode, and the specific operation mode is selected by an operator. When an operator selects an aerosol cleaning mode, the cleaning instrument operates the cleaning mode, under the cleaning mode, the equipment is only required to be pushed to any position of a laboratory, the equipment carries out automatic distance measurement (no obstacle exists at the corresponding position of a distance measurement assembly in each direction of the instrument to be ensured), the space volume of a PCR laboratory is automatically calculated, the required liquid adding amount of each experiment is automatically calculated, a cleaning agent in a liquid storage tank passes through an air compressor in an equipment box body, a peristaltic pump works, the cleaning agent is subjected to dry atomization through an atomizing head 22 and then is subjected to static reaction for 30 minutes, a fan of the equipment is automatically started afterwards, through an air inlet, an aerosol adsorption screen is automatically started, a photocatalyst reaction is carried out, the cleaning is carried out through an air outlet and a plasma generator, the whole cleaning process is completed, and the work of cleaning nucleic acid aerosol pollution is achieved. When an operator selects an adsorption purification mode, the equipment fan is automatically started, the aerosol adsorption screen and the photocatalyst reaction are automatically started through the air inlet, and the aerosol is discharged through the plasma generator at the air outlet, so that the whole physical and chemical removal process is completed, and the work of removing the nucleic acid aerosol pollution is achieved. The aerosol cleaning mode and the adsorption purification mode are completely and independently arranged, do not influence each other, and can be freely switched according to the laboratory conditions. The independent control operation is simpler, and an electric control unit for switching the running modes is not required to be designed.

The technical solution of the present invention is further explained with reference to fig. 1.

As shown in FIG. 1, the nucleic acid aerosol pollution remover comprises an outer shell, which comprises a front shell 101, a rear shell 1012, a middle shell 1013 and a bottom shell 1014, wherein four universal wheels 107 are arranged below the bottom shell 1014, so that the device can be moved conveniently. The top of the outer casing is provided with a high-definition touch screen 108 (on the middle casing 1013), a spray head support assembly 1031 and a spray head 22 on the support assembly, a protective cover 223 covers the spray head 22, the plasma air outlet cover plate 1091 is located above the middle casing 1013 and is provided with an air outlet grille, and the side of the middle casing 1013 is provided with an equipment switch 106. The high-definition touch screen 108 can be used for switching settings between a nucleic acid aerosol cleaning mode and an adsorption purification mode. The protective cover 223 is provided with a press sensing device, whether the protective cover 223 is opened or not can be automatically sensed, the protective spray head 22 is protected, and the press sensing device has a prompt and early warning function. The spray head 22 has a rotating function, and is convenient to disassemble, clean and replace. Air intake 11 is in a detachable door plant alone, and this door plant can dismantle with backshell 1012 and be connected, in a specific embodiment, can easily realize through backshell bounce switch 1021 dismantling the connection between door plant and the backshell 1012, and then be convenient for purify the maintenance and the change of adsorption component 5.

Laser rangefinder subassembly 6: the device is used for automatically measuring and calculating the volume of a room where the device is located, and five infrared laser ranging sensors are arranged on the front side, the rear side, the left side, the right side and the upper side of a device shell respectively. And measuring the volume of the room, and automatically converting the required liquid adding amount through an algorithm. A liquid storage tank 21: for storing a cleaning agent for cleaning, a reservoir holder 1033 for fixing the position of the reservoir, and a reservoir detector 105 for detecting the weight of the reservoir 21 provided below the reservoir holder. The front housing 101 of the device is provided with a rotatable door 1011 and is attached with a magnet device to facilitate the removal and replacement of the reservoir 21 into the housing. The filter screen is arranged at the position of the water outlet of the liquid storage tank 21, so that the scavenger is prevented from being blocked when flowing to the spray head 22 through the peristaltic pump.

Utilize air compressor machine 23 compressed air high-speed flow, atomize liquid through the atomising head 22 that lies in two dry fog nozzles, for preventing air compressor machine 23 long-time work, dispose 3 sets of radiator fan in the below for air compressor machine 23 during operation cooling.

It can be understood that the housing has a main frame 1092 for assembling the internal components therein, and accordingly, the air duct 3 and the fan 4 are assembled on the upper and middle regions of the main frame 1092, the bottom housing frame 1032 is located on the lower region of the main frame 1092, and the air compressor 23, the temperature control device 231 and the electronic module component 232 are all assembled on the bottom housing frame 1032. The purger also has an air quality sensor 104.

The fan 4 is used for extracting air in a laboratory room, the purifying and adsorbing assembly 5 integrating a photocatalyst and an aerosol adsorbing screen is arranged outside the fan 4, namely the photocatalyst is arranged on one side of the assembly, titanium dioxide generates a photocatalyst reaction under the illumination of the LED ultraviolet lamp assembly to generate hydroxyl radicals with super-strong oxidizing capability, and the aerosol adsorbing screen is arranged on one side of the air inlet. Air inlet is through purifying adsorption component 5, gets into inlet channel, and inside air can only get into the casing through inlet channel, the passageway setting of admitting air and giving vent to anger was on the channel subassembly, and air unidirectional flow is handled the back through plasma generator 121, is discharged by the air outlet apron, has set up the fence mouth on the air outlet board, the air-out of being convenient for and protection air outlet passageway. The purification adsorption module 5 is replaced periodically as required.

When the device is used, a special scavenger is configured, the liquid storage tank 21 is poured, the device is placed at any position without shielding in a laboratory, after the main interface is in light touch for 'clearing', the space measurement and calculation is clicked, the volume of a room in a PCR laboratory is automatically measured and calculated, a confirming key is clicked, and the volume of the room can also be manually set. After the space estimation interface clicks the "back" button, or manually enters the room volume, the system program automatically calculates the required amount of nucleic acid scavenger, and before operation, it must be ensured that more than the actual amount of nucleic acid scavenger is added. Then clicking a 'clear' button, the equipment is about to enter a running state, starting the system after counting down for 10 seconds, and when the equipment enters a nucleic acid aerosol clear mode state, a working state green light around the equipment flickers, which indicates that the equipment is in a normal working state. In the cleaning mode, the system can automatically operate the processes of a cleaning agent spraying stage, a standing action reaction stage, an adsorption screen physical adsorption stage and a photocatalyst reaction stage without manual staged re-operation. In the main menu of the screen, a 'setting' function key on the right side of the screen is clicked, and a setting interface is entered. Default factory settings are 10mL/min of flow, 30min of standing time, 30min of recovery time and 8mL/m of removal concentration ³ 。

When this implementation was used, "adsorb to purify" of the screen left side below, start and adsorb purification performance, equipment work pilot lamp is blue scintillation state, adsorbs to purify the work and accomplishes the back, and equipment work pilot lamp is blue state of often brightening. The photocatalyst function and the adsorption function can coexist in a man-machine mode, and the action duration can be modified on a program editing interface. The adsorption and purification work is finished, and the equipment work indicator lamp is in a blue normally-on state.

The automatic cleaning mode and the adsorption mode can be freely switched through the high-definition touch screen 108.

It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, it is possible to make several improvements and modifications without departing from the technical principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.

Claims (10)

1. The nucleic acid aerosol pollution eliminating instrument is characterized by comprising an outer shell, wherein an air inlet (11) and an air outlet (12) are formed in the outer shell, a liquid storage tank (21) for storing a nucleic acid aerosol scavenger, a spraying head (22), an air duct (3) and a fan (4) in the air duct (3) are arranged in the outer shell, the fan (4) can drive air flow of an external environment to flow into the air duct (3) through the air inlet (11) and flow out from the air outlet (12), scavenger in the liquid storage tank (21) can be pumped to the position of the spraying head (22) to form dry mist spraying, and the sprayed dry mist can flow along with the air flow flowing out of the air outlet (12).

2. The nucleic acid aerosol pollution remover according to claim 1, wherein the spray head (22) is provided with two symmetrically arranged scavenger spray nozzles (221) and an air flow nozzle (222) between the two scavenger spray nozzles (221), the ejection paths of the two symmetrically arranged scavenger spray nozzles (221) and the ejection path of the air flow nozzle (222) intersect at the same point, an air compressor (23) is further arranged in the outer shell, and the exhaust port of the air compressor (23) is communicated with the air flow nozzle (222).

3. The nucleic acid aerosol pollution removal instrument according to claim 2, wherein a temperature control device (231) is further provided adjacent to the air compressor (23), and the temperature control device (231) is used for cooling the air compressor (23).

4. The nucleic acid aerosol pollution removal instrument according to claim 2, wherein the fan (4) is a centrifugal fan, an air suction port of the centrifugal fan is arranged opposite to the air inlet (11), an air outlet of the centrifugal fan is arranged opposite to the air outlet (12) through the air duct (3), the number of the air outlets (12) is two, the air duct (3) has two parallel sub-channels, an outlet of each sub-channel corresponds to one of the air outlets (12), and the spray head (22) is located at a cross-collection position of the two sub-channels and outside the sub-channels.

5. The nucleic acid aerosol pollution removal instrument according to claim 4, wherein a plasma generator (121) is respectively arranged in each air outlet (12); and/or a protective cover (223) is covered on the spray head (22).

6. The nucleic acid aerosol pollution removal instrument according to claim 1, wherein a purification and adsorption component (5) is further arranged between the air inlet (11) and the air suction opening of the fan (4).

7. The nucleic acid aerosol pollution removal instrument according to claim 6, wherein one side of the purification and adsorption component (5) is an aerosol adsorption screen, the other side of the purification and adsorption component is a photocatalyst structure, and the photocatalyst structure is located on one side of the purification and adsorption component (5) far away from the air inlet (11).

8. The nucleic acid aerosol pollution remover according to claim 2, further comprising a laser ranging assembly (6) for detecting the volume of the space where the nucleic acid aerosol pollution remover is located; and/or the liquid storage tank (21) is communicated with the scavenger spray nozzle (221) in parallel through a peristaltic pump, and an anti-blocking filter screen is arranged on a pipeline between the peristaltic pump and the liquid storage tank (21).

9. A control method for a nucleic acid aerosol pollution removing apparatus according to any one of claims 2 to 8, comprising the steps of:

obtaining the operation mode of the nucleic acid aerosol pollution removing instrument;

when the operation mode is the aerosol removal mode, the peristaltic pump, the air compressor (23) and the fan (4) are controlled to operate, the operation is stopped for a second preset time after the operation is carried out for a first preset time, and then the fan (4) is started again and the photocatalyst structure and the plasma generator (121) are controlled to operate;

and when the operation mode is the adsorption purification mode, controlling the photocatalyst structure, the plasma generator (121) and the fan (4) to operate.

10. The control method according to claim 9, wherein before the aerosol removal mode is operated, volume information of a space where the nucleic acid aerosol contamination remover is located is acquired, and a filling amount of the nucleic acid aerosol remover in the liquid storage tank (21) is calculated based on the acquired volume information.

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