CN219194980U - Algae culture experimental device for controlling water body flow - Google Patents
Algae culture experimental device for controlling water body flow Download PDFInfo
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- CN219194980U CN219194980U CN202320285126.2U CN202320285126U CN219194980U CN 219194980 U CN219194980 U CN 219194980U CN 202320285126 U CN202320285126 U CN 202320285126U CN 219194980 U CN219194980 U CN 219194980U
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Abstract
The utility model discloses an algae culture experimental device for controlling water flow, wherein an algae culture container is arranged on a container base, and an algae culture solution is filled in the algae culture container; the top of the algae culture container is provided with a top cover, a water body flowing stirring rod is arranged below the top cover, and a rotating motor is arranged above the top cover; the water body flowing stirring rod is connected with the rotating motor through a stirring rod transmission shaft, and stirring rod fan blades are fixed on the peripheral surface of the water body flowing stirring rod; the rotary motor is sequentially connected with the controller and the wire plug through the wires of the controller; a top cover pad is arranged below the top cover, so that a gap is reserved between the top cover and the opening of the algae cultivation container. The device can realize the uniform circulation flow of the water body during the algae cultivation, can realize constant illumination and temperature when being put into an illumination incubator, has easily-adjustable flow rate of the control device, and is beneficial to algae cultivation.
Description
Technical Field
The utility model belongs to a biological experimental device, and particularly relates to an algae culture experimental device for controlling water flow.
Background
Algae experiments are usually carried out in a traditional illumination incubator by adopting a conical flask, but the mass proliferation of algae in natural water is influenced by water flow, and the water flow in the flask is difficult to control by the traditional conical flask, so that the influence of the change of the water flow on the growth of algae cannot be accurately explored. In addition, the algae cultivation industry usually uses an annular water tank to cultivate, and the water body in the water tank is driven by a motor to flow so as to promote the proliferation of algae. Part of algae culture devices are designed to rotate by single fan blades to drive water bodies to rotate, but the water bodies in the culture devices are easy to rotate in a vortex shape, and the flowing environment in the water bodies has larger anisotropism.
Disclosure of Invention
The utility model aims to: aiming at the problems existing in the prior art, the utility model provides an algae cultivation experimental device for controlling the water body to flow, which comprises but is not limited to a small device size and no limitation of experimental cultivation scenes, and meanwhile, a control device is also arranged, the rotating speed of a motor is controlled by a controller, the rotating drum in the driving device realizes constant-speed rotation, so that the state that the algae cultivation water body flows to achieve dynamic balance can be achieved, the experimental device is easy to sample and test, and the aim of exploring the influence of different water body flows on the growth and proliferation states of algae is achieved.
The technical scheme is as follows: in order to achieve the aim, the algae culture experimental device for controlling the water body flow mainly comprises a controller, a rotary motor, a water body flow stirring rod and an algae culture container. The controller can be according to the water flow demand, through the required flow operating mode of knob rapid change, connect the water flow stirring rod under the rotary motor, through the rotation drive water flow stirring rod of rotary motor, the algae culture container then reaches not limited and the purpose of easy sample test in place.
The specific technical scheme is as follows:
an algae culture experimental device for controlling water flow comprises an algae culture container, wherein the algae culture container is arranged on a container base, and an algae culture solution is filled in the algae culture container;
the top of the algae culture container is provided with a top cover, a water body flowing stirring rod is arranged below the top cover, and a rotating motor is arranged above the top cover; the water body flowing stirring rod is connected with the rotating motor through a stirring rod transmission shaft, and stirring rod fan blades are fixed on the peripheral surface of the water body flowing stirring rod; the rotary motor is sequentially connected with the controller and the wire plug through the wires of the controller;
a top cover pad is arranged below the top cover, so that a gap is reserved between the top cover and the opening of the algae cultivation container.
The controller adopts an eight-bit dial-up easy-to-pull plug switch to connect the controller wire with the controller.
The rotary motor is connected with the stirring rod transmission shaft through a buckle, and is easy to detach and replace.
The stirring rod transmission shaft is connected with the water body flowing stirring rod by a threaded knob, and the water body flowing stirring rod is fixedly connected with the stirring rod fan blade; the water body flowing stirring rod is of a hollow structure, and the gravity center is adjusted by adding fillers.
Specifically, the algae culture experimental device for controlling the water flow comprises a controller (knob control), a rotary motor with a rotary function, a water flow stirring rod and an algae culture container filled with algae culture solution; the controller is 3X 0.75mm through national standard AC220V tripod 2 The copper core power line of the motor is connected to an indoor 220V power supply, and the other side of the copper core power line is connected to a rotating motor with a direct-current gear reduction function; the rotary motor is connected to the starter port of the controller through 5 wires, and the other side of the rotary motor is connected to the water body flowing stirring rod through a stainless steel column; the stirring rod fan blades are arranged around the water body flowing stirring rod to control the water body in the algae culture container to rotate and flow, so that the water body flow speed is changed.
Preferably, the controller is a servo motor stepping motor pulse speed regulation controller with a knob.
The controller is provided with a rotatable knob with 6 gears, and the water flow rate can be controlled to be 0, 0.1, 0.2, 0.3, 0.4 and 0.5m/s through the knob.
Wherein, the left end of the controller is provided with an alternating current interface which is 3 multiplied by 0.75mm by three feet of national standard AC220V 2 Is connected to an indoor 220V power supply.
The right end of the controller is provided with a 5-wire junction box, current is output into direct current through a rectifier in the controller, voltage output is 24, 36, 48DC and the like, and the current range is 0.1-5.6A.
Preferably, the rotary motor is a direct-current gear reduction motor device.
The controller is provided with a high-hardness buckle bearing and high-efficiency radiating fins, and the rotating speed of the motor bearing can be controlled to be 50-850 revolutions per minute.
Wherein, high rigidity buckle bearing connect with the stainless steel bar and constitute wholly.
Preferably, the water body flowing stirring rod is provided with a detachable stirring rod (barrel), stirring rod fan blades and a top cover with a top cover pad, and can rotate at a constant speed in an electric water body circulating way.
The stirring rod (barrel) is connected with the upper thread cover, the thread cover is connected with the stainless steel rod, the stirring rod (barrel) is of a hollow structure, the stirring rod (barrel) can be filled with heavy objects (sand, broken stone) and the like to increase rotational inertia, stirring rod fan blades with the width of 4 bars being 1cm are adhered to the periphery of the side of the stirring rod (barrel), a round baffle plate with the width of 2cm than that of the stirring rod (barrel) is adhered to the bottom of the stirring rod (barrel), and the baffle plate is parallel to the bottom plate of the algae culture container and is higher than the bottom plate of the algae culture container by 5mm.
Wherein, the top cap is connected with the top cap pad of 4 places 1cm height, when the top cap emboliaed the algae culture container along with the stirring rod of water flow for top cap and algae culture container keep 1cm space, and water flow stirring rod bottom is 5mm higher than the algae culture container bottom plate apart from the bottom plate.
Preferably, the algae cultivation vessel is provided with a cylinder and a bottom plate, and the volume is about 5L. The algae cultivation vessel is placed on the vessel base. The cylinder consists of an acrylic plate, the light transmission of the material does not influence photosynthesis in the algae cultivation process, and the diameter of the cylinder is 16cm.
Wherein, the bottom plate also comprises an acrylic plate, the shape is rectangle, and the length and the width are 20cm.
Working principle: the utility model can control and regulate the illumination intensity required by culture through the illumination incubator, and change the illumination period. The algae culture experimental device for controlling the water flow rate is placed in an illumination incubator by 18 groups (3 groups are a target flow rate), 18 controllers are correspondingly arranged in the incubator, 54L of experiment culture medium with the same concentration required by the experiment is configured, 3L of culture medium is poured into each device to serve as algae culture solution, and water flow stirring rods are covered one by one. The temperature and the humidity are controlled and regulated in the illumination incubator according to the required temperature and humidity, after the device is installed, a power supply is connected, a controller knob is turned on, and the flow speed is regulated, so that the algae liquid in the algae culture container flows uniformly. The working principle and sequence of each group are the same as the above. After the water temperature of the culture medium is basically kept constant and consistent, inoculating target test organisms with the same algae density into a gap at the lower part of a top cover of each experimental device, setting the light irradiation device to be light-dark ratio after the inoculation is finished, and starting continuous flow culture for a long time. At the beginning of the experiment, culture medium can be supplemented into the algae culture container through the soft rubber tube, and algae samples can be taken out for testing by utilizing the siphon principle.
The illumination device is turned on immediately after the algae cell inoculation is completed, and the light-dark ratio is adjusted to be 12:12h.
The beneficial effects are that: compared with the prior art, the utility model has the following advantages:
compared with the conventional method for culturing algae cells in an illumination incubator by using a conical flask, the annular water tank device has large occupied space and uneven flow velocity in the device because the conical flask can only generate water flow by shaking, the experimental device can realize water flow condition control during algae culture, the flow condition required by algae growth is molded by connecting a controller, a direct current motor, a stirring rod device and a container of a culture medium, the supplement of the culture medium and the sampling of algae samples can be completed from a gap of 1cm at the bottom of a cover plate of the stirring device, the flow velocity and the concentration in the culture medium are maintained unchanged, meanwhile, the illumination and temperature modes can be controlled like a common conical flask, the illumination period (light-dark ratio) is changed, and the temperature change is controlled.
Compared with the control of water flow by using independent fan blades, the experimental device uses the detachable rod-shaped stirring rod, the weight of the stirring rod can be set according to independent experimental requirements, the volume of a culture medium and other variables so as to overcome the buoyancy in the stirring process, and the diameter of the stirring rod can be independently replaced so as to meet the setting requirements of the water flow rate and different turbulent conditions in the water flow; in addition, the stirring rod is provided with the fan blades at the periphery, so that the vertical flow consistency in the experimental device is ensured, and the accurate control of the algae culture and flow environment is facilitated.
The utility model also provides a gap of 1cm between the top cover and the algae culture container, so that on one hand, the top is prevented from being perforated to prevent air from falling into other spores in the experimental process, and on the other hand, the operations of continuously adding culture medium, algae sampling and the like can be simultaneously satisfied, and convenience of algae experiments is satisfied.
The utility model has simple, practical and convenient structure, can control the flow speed and the turbulence, is also provided with the detachable stirring rod and the 1cm gap, avoids the incapability of meeting the culture experiment requirements of different algae, can achieve the aim that the water flow in the algae culture process is kept constant through the external controller so as to achieve a dynamic balance state, is not easy to be polluted by other impurities, is arranged in the illumination incubator so as to meet the requirement of limited space, and achieves the aim of singly controlling the water flow to explore the influence of the water flow on the algae growth dynamics.
Drawings
FIG. 1 is a schematic diagram of a combined structure of a device for controlling water flow according to the present utility model;
FIG. 2 is a schematic diagram of a split structure of a device for controlling water flow according to the present utility model;
FIG. 3a is a schematic illustration of the results of a simulation of the flow of water in a device of an algae cultivation process;
FIG. 3b is a second schematic diagram of the simulation result of the water flow state in the device of the algae cultivation process;
FIG. 3c is a third schematic view of the results of the simulation of the water flow conditions in the device of the algae cultivation process.
Description of the embodiments
The utility model is further described below with reference to the drawings and examples.
As shown in fig. 1 and 2, an algae cultivation experiment device for controlling water flow specifically comprises an algae culture solution 1, an algae cultivation container 2, a container base 3, a water flow stirring rod 4, stirring rod fan blades 5, a top cover pad 6, a stirring rod transmission shaft 7, a top cover 8, a rotation motor 9, a controller 10, a controller wire 11 and a wire plug 12.
The utility model discloses need assemble before the device experiments, algae culture solution 1, algae culture vessel 2, container base 3 are the integral type generally, and water flows stirring rod 4, stirring rod fan blade 5, top cap pad 6, stirring rod transmission shaft 7, top cap 8, rotation motor 9, controller 10, controller electric wire 11, electric wire plug 12 are the integral type, as shown in fig. 1. The algae culture liquid 1 and algae need to be added into the algae culture container 2, and the adding volume is usually determined by the volume to be sampled in the whole process of the experiment, and the total volume of the whole process to be sampled is not more than 5% of the total liquid.
The controller 10, the controller wire 11 and the wire plug 12 are of non-detachable design, the rotary motor 9 and the controller 10 adopt eight-bit dial easily-pulled and plugged switches, and the controller wire 11 is connected with the controller 10; wherein the rotary motor 9 is connected with the stirring rod transmission shaft 7 through a buckle, so that the stirring rod is easy to detach and replace; wherein stirring rod transmission shaft 7 and water flow stirring rod 4 adopt screw knob to connect, and water flow stirring rod 4 and stirring rod fan blade 5 are the glass gluey adhesion, and is undetachable, and during the experimental apparatus equipment, water flow stirring rod 4 is through adding steel ball, rubble and cotton piece etc. and adjusting focus to satisfy the demand of different experiments, with the example of adding 3L culture medium, then need add 150g loose rubble in order to satisfy stirring demand, and satisfy motor's rated power.
After the algae sample measurement volume is calculated, 3L of culture medium is required to be added in each group of experiments, 6 different flow rate working conditions (each group corresponds to different water flow rates) are set in the experiments, 3 parallel samples are required to be set in each group of flow rates, an acrylic plate can be adopted as a main material for manufacturing the experimental device, the price is substantial, and 18 experimental devices are set in the same batch. 3L of algae culture medium is filled into each experimental device to serve as an algae culture solution 1, a water body flowing stirring rod 4 is buckled above an algae culture container 2, the algae culture solution is placed into an illumination incubator, the temperature is set to 25 ℃, the relative humidity is set to 85%, and the illumination intensity is set to 6000lx in an illumination period (illumination and temperature setting can be changed according to the experiment requirement). After all the devices are placed in an illumination incubator and the electric wire plugs 12 are connected with a 220V power supply, the knob of the controller 10 is rotated, the flow conditions of 6 groups are respectively set to 0, 0.1, 0.2, 0.3, 0.4 and 0.5m/s, the turbulence condition of the water body in the devices is inverted based on numerical simulation software such as flow 3d, and the like, as shown in fig. 3 a-3 c, the water body flow in the devices is uniform everywhere, and the conditions for controlling the water body flow can be met. After the water temperature of the culture medium is basically kept constant and consistent, the microcystis aeruginosa stock solution with the same algae density (target organisms can be replaced according to the experiment requirement) is inoculated into the gap at the lower part of the top cover 8 of each experimental device, and after the inoculation is finished, the illumination device is set to be light-dark ratio, and continuous flow culture is started for a long time. The illumination device is turned on immediately after the algae cell inoculation is completed, and the light-dark ratio is adjusted to be 12:12h. At the beginning of the experiment, the culture medium can be supplemented into the algae culture container 2 through the soft rubber tube, and the algae sample can be taken out for testing by utilizing the siphon principle. The sample measurement in the experimental process is also synchronously performed, and the sample measurement work is started simultaneously when the switch of the controller 10 is initially turned on, so that the algae reaction and the experimental index test reach a synchronous equilibrium state.
What has been described above is merely some embodiments of the present utility model. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit of the utility model.
Claims (4)
1. The algae culture experimental device for controlling the water body to flow is characterized by comprising an algae culture container (2), wherein the algae culture container (2) is arranged on a container base (3), and an algae culture solution (1) is filled in the algae culture container (2);
a top cover (8) is arranged at the top of the algae culture container (2), a water body flowing stirring rod (4) is arranged below the top cover (8), and a rotating motor (9) is arranged above the top cover; the water body flowing stirring rod (4) is connected with a rotating motor (9) through a stirring rod transmission shaft (7), and stirring rod fan blades (5) are fixed on the peripheral surface of the water body flowing stirring rod (4); the rotary motor (9) is sequentially connected with the controller (10) and the wire plug (12) through the controller wire (11);
a top cover pad (6) is arranged below the top cover (8) so that a gap is reserved between the top cover (8) and the mouth part of the algae cultivation container (2).
2. The experimental device for culturing algae for controlling water flow according to claim 1, wherein the controller (10) adopts an eight-bit dial-up easy-to-draw switch to connect the controller wire (11) with the controller (10).
3. The algae cultivation experiment device for controlling water flow according to claim 1, wherein the rotary motor (9) is connected with the stirring rod transmission shaft (7) through a buckle, and is easy to detach and replace.
4. The experimental device for culturing algae for controlling water flow according to claim 1, wherein the stirring rod transmission shaft (7) is connected with the water flow stirring rod (4) by a threaded knob, and the water flow stirring rod (4) is fixedly connected with the stirring rod fan blades (5); the water body flowing stirring rod (4) is of a hollow structure, and the gravity center is adjusted by adding fillers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320285126.2U CN219194980U (en) | 2023-02-22 | 2023-02-22 | Algae culture experimental device for controlling water body flow |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320285126.2U CN219194980U (en) | 2023-02-22 | 2023-02-22 | Algae culture experimental device for controlling water body flow |
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| Publication Number | Publication Date |
|---|---|
| CN219194980U true CN219194980U (en) | 2023-06-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320285126.2U Active CN219194980U (en) | 2023-02-22 | 2023-02-22 | Algae culture experimental device for controlling water body flow |
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| CN (1) | CN219194980U (en) |
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2023
- 2023-02-22 CN CN202320285126.2U patent/CN219194980U/en active Active
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