CN214612537U - Simulation culture device for stem cells - Google Patents
Simulation culture device for stem cells Download PDFInfo
- Publication number
- CN214612537U CN214612537U CN202023239445.5U CN202023239445U CN214612537U CN 214612537 U CN214612537 U CN 214612537U CN 202023239445 U CN202023239445 U CN 202023239445U CN 214612537 U CN214612537 U CN 214612537U
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- device main
- main body
- bottom plate
- swing joint
- telescopic link
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 30
- 238000004088 simulation Methods 0.000 title abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 34
- 230000008878 coupling Effects 0.000 claims abstract description 8
- 238000010168 coupling process Methods 0.000 claims abstract description 8
- 238000005859 coupling reaction Methods 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 238000004113 cell culture Methods 0.000 abstract description 15
- 230000008859 change Effects 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 description 7
- 230000009471 action Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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Abstract
The utility model discloses a stem cell's emulation culture apparatus, including the device main part, the inside top of device main part is provided with the pneumatic cylinder, the lower extreme port of pneumatic cylinder is provided with the piston rod, the lower extreme of piston rod is provided with servo motor, servo motor's lower extreme is provided with the shaft coupling, the bottom surface left and right sides of shaft coupling all is provided with the rotation axis, the lower extreme of rotation axis is provided with stirring vane, the inside bottom surface of device main part is provided with the bottom plate, the upper end of bottom plate is provided with places the board, it is provided with the support column to place between board and the bottom plate, the middle part of the upper end surface of bottom plate is located and is provided with the telescopic link between the support column, the upper end port of telescopic link is provided with the fly leaf. The utility model discloses a simulation culture apparatus of stem cell has better cell culture liquid stirring effect, avoids nutrient solution maldistribution to influence stem cell culture effect, has the effect that the culture dish takes out fast simultaneously, makes things convenient for the change of culture dish.
Description
Technical Field
The utility model relates to a cell culture technical field, in particular to stem cell's emulation culture apparatus.
Background
Cell culture has been an indispensable important part in the scientific research process now, has great meaning to bio-pharmaceuticals, animal and plant experimental engineering, however current stem cell's emulation culture apparatus does not have cell culture liquid stirring effect, and cell culture liquid distributes unevenly when stem cell culture, causes the cell to distribute unevenly easily to influence the cell culture effect, the culture dish is placed and is difficult for taking out in the standing groove simultaneously, is unfavorable for the quick replacement of culture dish, for this reason, we propose a stem cell's emulation culture apparatus.
SUMMERY OF THE UTILITY MODEL
The main object of the utility model is to provide a stem cell's emulation culture apparatus, can effectively solve the problem in the background art.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a simulation culture device of stem cells comprises a device main body, wherein a hydraulic cylinder is arranged above the inside of the device main body, a piston rod is arranged at the lower end port of the hydraulic cylinder, a servo motor is arranged at the lower end of the piston rod, the lower end of the servo motor is provided with a coupler, the left side and the right side of the bottom surface of the coupler are both provided with rotating shafts, the lower end of the rotating shaft is provided with a stirring blade, the bottom surface in the device main body is provided with a bottom plate, a placing plate is arranged at the upper end of the bottom plate, a supporting column is arranged between the placing plate and the bottom plate, the middle part of the upper end surface of the bottom plate is provided with a telescopic rod between the support columns, the upper end port of the telescopic rod is provided with a movable plate, the device comprises a movable plate and is characterized in that a top column is arranged on the upper end surface of the movable plate, a placing groove is formed in the upper end surface of the placing plate, and a PLC is arranged on the right side surface of the device body.
Preferably, be swing joint between pneumatic cylinder and the device main part, be swing joint between piston rod and the pneumatic cylinder, be equal swing joint between servo motor and piston rod and the shaft coupling, be swing joint between rotation axis and the stirring vane, be electric connection between servo motor and the PLC controller.
By adopting the technical scheme, the following technical effects can be achieved: is beneficial to the stirring of the culture solution and ensures that the culture solution is uniformly distributed.
Preferably, the rotating shaft and the stirring blades are positioned right above the placing groove, the number of the stirring blades is a plurality of groups, and the stirring blades are arranged in an array.
By adopting the technical scheme, the following technical effects can be achieved: is favorable for improving the stirring effect.
Preferably, be swing joint between bottom plate and the telescopic link, be swing joint between telescopic link and the fly leaf, be fixed connection between fore-set and the fly leaf, the bottom of standing groove is provided with the expansion mouth, the fore-set passes the expansion mouth and extends to the inside bottom surface of standing groove, be swing joint between fore-set and the standing groove, the telescopic link is automatic telescopic link, be electric connection between telescopic link and the PLC controller.
By adopting the technical scheme, the following technical effects can be achieved: is beneficial to the quick taking out of the culture dish.
Preferably, the placing groove is fixedly connected with the placing plate, a rubber pad is arranged on the inner side face of the placing groove, and the rubber pad is movably connected with the placing groove.
By adopting the technical scheme, the following technical effects can be achieved: is beneficial to the protection of the culture dish.
Preferably, a scavenging valve is arranged on the upper end surface of the device main body, a temperature sensor is arranged on the left side surface of the device main body, a constant temperature heating box is arranged on the right side surface of the device main body, the constant temperature heating box, the temperature sensor and the PLC are electrically connected, an observation window is arranged on the front end surface of the device main body, and the observation window is movably connected with the device main body.
By adopting the technical scheme, the following technical effects can be achieved: the method is favorable for providing a simulated constant temperature environment.
Compared with the prior art, the utility model discloses following beneficial effect has: the simulation culture device for the stem cells has the advantages that under the combined action of the hydraulic cylinder, the piston rod, the servo motor, the coupler, the rotating shaft and the stirring blade, when the stem cells are cultured in the simulation culture device, the hydraulic cylinder pushes the piston rod to move downwards to enable the piston rod to drive the servo motor, the coupler and the rotating shaft to move downwards together, when the stirring blade is located in a culture dish on the placing plate, the PLC controls the servo motor to rotate at a slow speed, the rotating shaft drives the stirring blade to rotate slowly to stir culture solution in the culture dish, so that the culture solution is uniformly distributed, a good cell culture solution stirring effect is achieved, and the influence on the culture effect of the stem cells due to the fact that the nutrient solution is not uniformly distributed is avoided; through the combined action of telescopic link, fly leaf, fore-set and expansion gap, the stem cell culture dish is put into and is fixed in placing the standing groove on the board, and the rubber pad culture dish protects, and when the cultivation was finished, PLC controller control telescopic link upwards stretches out and draws back and drives the fly leaf and shift up, and then makes the fore-set pass the expansion gap and extend to inside the slow ejecting standing groove of culture dish with the standing groove, is convenient for take out fast to have the effect that the culture dish takes out fast, make things convenient for the change of culture dish.
Drawings
FIG. 1 is a schematic view of the overall structure of a simulated stem cell culture apparatus according to the present invention;
FIG. 2 is a front sectional view of a stem cell simulation culture apparatus according to the present invention;
FIG. 3 is an enlarged view of A in FIG. 2 of a simulated stem cell culturing apparatus according to the present invention;
FIG. 4 is a sectional view of a part of the structure of a simulated stem cell culture apparatus according to the present invention.
In the figure: 1. a device main body; 2. an observation window; 3. a temperature sensor; 4. a constant temperature heating box; 5. a PLC controller; 6. a scavenging valve; 7. a hydraulic cylinder; 8. a piston rod; 9. a servo motor; 10. a coupling; 11. a rotating shaft; 12. a stirring blade; 13. placing the plate; 14. a support pillar; 15. a telescopic rod; 16. a movable plate; 17. a top pillar; 18. a placement groove; 19. a rubber pad; 20. a movable opening; 21. a base plate.
Detailed Description
In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.
As shown in fig. 1-4, a stem cell simulation culture device comprises a device body 1, a hydraulic cylinder 7 is arranged above the inside of the device body 1, a piston rod 8 is arranged at the lower end port of the hydraulic cylinder 7, a servo motor 9 is arranged at the lower end of the piston rod 8, a coupler 10 is arranged at the lower end of the servo motor 9, rotating shafts 11 are arranged on the left side and the right side of the bottom surface of the coupler 10, stirring blades 12 are arranged at the lower end of the rotating shafts 11, so as to facilitate stirring of a culture solution and enable the culture solution to be uniformly distributed, a bottom plate 21 is arranged at the bottom inside of the device body 1, a placing plate 13 is arranged at the upper end of the bottom plate 21, supporting columns 14 are arranged between the placing plate 13 and the bottom plate 21, telescopic rods 15 are arranged between the supporting columns 14 at the middle part of the upper end surface of the bottom plate 21, a movable plate 16 is arranged at the upper end port of the telescopic rods 15, and a top column 17 is arranged on the upper end surface of the movable plate 16, the culture dish is convenient to eject, a placing groove 18 is formed in the upper end surface of the placing plate 13, and the PLC controller 5 is arranged on the right side surface of the device main body 1.
For swing joint between pneumatic cylinder 7 and the device main part 1, be swing joint between piston rod 8 and the pneumatic cylinder 7, for equal swing joint between servo motor 9 and piston rod 8 and the shaft coupling 10, be swing joint between rotation axis 11 and stirring vane 12, be electric connection between servo motor 9 and the PLC controller 5, be favorable to the stirring of culture solution, make the culture solution distribute evenly.
For swing joint between bottom plate 21 and the telescopic link 15, be swing joint between telescopic link 15 and the fly leaf 16, be fixed connection between fore-set 17 and the fly leaf 16, the bottom of standing groove 18 is provided with expansion mouth 20, fore-set 17 passes expansion mouth 20 and extends to the inside bottom surface of standing groove 18, be swing joint between fore-set 17 and the standing groove 18, telescopic link 15 is automatic telescopic link, be electric connection between telescopic link 15 and the PLC controller 5, be favorable to taking out fast of culture dish.
For fixed connection between standing groove 18 and the standing plate 13, the inside side of standing groove 18 is provided with rubber pad 19, is swing joint between rubber pad 19 and the standing groove 18, is favorable to the protection of culture dish, avoids the breakage.
The upper end surface of device main part 1 is provided with breather valve 6, be convenient for take a breath, the left side surface of device main part 1 is provided with temperature sensor 3, the right side surface of device main part 1 is provided with constant temperature heating cabinet 4, be electric connection between constant temperature heating cabinet 4 and temperature sensor 3 and the PLC controller 5, be favorable to providing constant temperature simulation environment, the front end surface of device main part 1 is provided with observation window 2, be swing joint between observation window 2 and the device main part 1, be convenient for observe the cultivation condition.
It should be noted that, the utility model relates to a stem cell's emulation culture apparatus, when using, the user is with the help of pneumatic cylinder 7, piston rod 8, servo motor 9, shaft coupling 10, the combined action of rotation axis 11 and stirring vane 12, stem cell is when emulation culture apparatus is inside to be cultivateed, pneumatic cylinder 7 promotes piston rod 8 and moves down and make piston rod 8 drive servo motor 9, shaft coupling 10 and rotation axis 11 move down together, when stirring vane 12 was located the culture dish on placing plate 13, PLC controller 5 control servo motor 9 rotated with slow speed, rotation axis 11 drives stirring vane 12 slow turning and stirs the culture solution in the culture dish, make the culture solution evenly distributed, thereby have better cell culture solution stirring effect, avoid nutrient solution to distribute the inequality and influence the stem cell culture effect; through telescopic link 15, the fly leaf 16, the combined action of fore-set 17 and expansion gap 20, the stem cell culture dish is put into the standing groove 18 on placing the board 13 and is fixed, rubber pad 19 culture dish protects, when the cultivation is finished, PLC controller 5 control telescopic link 15 upwards stretches out and draws back and drives fly leaf 16 and shifts up, and then make the fore-set 17 pass the expansion gap 20 and extend to the standing groove 18 inside with the slow ejecting standing groove 18 of culture dish, be convenient for take out fast, thereby have the effect that the culture dish takes out fast, make things convenient for the change of culture dish.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A simulated culture device of stem cells, comprising a device body (1), characterized in that: a hydraulic cylinder (7) is arranged above the inside of the device main body (1), a piston rod (8) is arranged at a lower end port of the hydraulic cylinder (7), a servo motor (9) is arranged at the lower end of the piston rod (8), a coupler (10) is arranged at the lower end of the servo motor (9), rotating shafts (11) are arranged on the left side and the right side of the bottom surface of the coupler (10), stirring blades (12) are arranged at the lower end of the rotating shafts (11), a bottom plate (21) is arranged at the bottom inside the device main body (1), a placing plate (13) is arranged at the upper end of the bottom plate (21), supporting columns (14) are arranged between the placing plate (13) and the bottom plate (21), telescopic rods (15) are arranged between the supporting columns (14) in the middle of the upper end surface of the bottom plate (21), and movable plates (16) are arranged at upper end ports of the telescopic rods (15), the upper end surface of the movable plate (16) is provided with a top column (17), the upper end surface of the placing plate (13) is provided with a placing groove (18), and the right side surface of the device main body (1) is provided with a PLC (programmable logic controller) (5).
2. The simulated culture device of stem cells according to claim 1, wherein: be swing joint between pneumatic cylinder (7) and device main part (1), be swing joint between piston rod (8) and pneumatic cylinder (7), be equal swing joint between servo motor (9) and piston rod (8) and shaft coupling (10), be swing joint between rotation axis (11) and stirring vane (12), be electric connection between servo motor (9) and PLC controller (5).
3. The simulated culture device of stem cells according to claim 1, wherein: the rotary shaft (11) and the stirring blades (12) are located right above the placing groove (18), the number of the stirring blades (12) is a plurality of groups, and the stirring blades (12) are arranged in an array.
4. The simulated culture device of stem cells according to claim 1, wherein: be swing joint between bottom plate (21) and telescopic link (15), be swing joint between telescopic link (15) and fly leaf (16), be fixed connection between fore-set (17) and fly leaf (16), the bottom of standing groove (18) is provided with expansion mouth (20), fore-set (17) pass expansion mouth (20) and extend to the inside bottom surface of standing groove (18), be swing joint between fore-set (17) and standing groove (18), telescopic link (15) are automatic telescopic link, be electric connection between telescopic link (15) and PLC controller (5).
5. The simulated culture device of stem cells according to claim 1, wherein: the placing groove (18) is fixedly connected with the placing plate (13), a rubber pad (19) is arranged on the inner side face of the placing groove (18), and the rubber pad (19) is movably connected with the placing groove (18).
6. The simulated culture device of stem cells according to claim 1, wherein: the device is characterized in that a ventilation valve (6) is arranged on the upper end surface of the device main body (1), a temperature sensor (3) is arranged on the left side surface of the device main body (1), a constant-temperature heating box (4) is arranged on the right side surface of the device main body (1), the constant-temperature heating box (4), the temperature sensor (3) and the PLC (programmable logic controller) (5) are electrically connected, an observation window (2) is arranged on the front end surface of the device main body (1), and the observation window (2) is movably connected with the device main body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023239445.5U CN214612537U (en) | 2020-12-29 | 2020-12-29 | Simulation culture device for stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023239445.5U CN214612537U (en) | 2020-12-29 | 2020-12-29 | Simulation culture device for stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214612537U true CN214612537U (en) | 2021-11-05 |
Family
ID=78433151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023239445.5U Expired - Fee Related CN214612537U (en) | 2020-12-29 | 2020-12-29 | Simulation culture device for stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214612537U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114621853A (en) * | 2022-05-16 | 2022-06-14 | 广州大学 | Microbial cultivation device |
-
2020
- 2020-12-29 CN CN202023239445.5U patent/CN214612537U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114621853A (en) * | 2022-05-16 | 2022-06-14 | 广州大学 | Microbial cultivation device |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211105 |
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| CF01 | Termination of patent right due to non-payment of annual fee |