CN219185891U

CN219185891U - Stem cell factor extraction device

Info

Publication number: CN219185891U
Application number: CN202222288922.XU
Authority: CN
Inventors: 李一佳; 苏耀耀; 胡冬冰; 张萌; 郭俊松
Original assignee: Hangzhou Qingda Kerui Biotechnology Co ltd
Current assignee: Hangzhou Qingda Kerui Biotechnology Co ltd
Priority date: 2022-08-30
Filing date: 2022-08-30
Publication date: 2023-06-16
Anticipated expiration: 2032-08-30

Abstract

The utility model relates to the field of cytokine extraction, and discloses a stem cell factor extraction device, which is characterized in that: comprises a collecting bottle, a reaction bottle and an extraction device; the lower end of the extraction device is provided with the reaction bottle; the collecting bottle is arranged at one side of the extraction device and one side of the reaction bottle; the utility model can promote stem cells to quickly produce cytokines, and can well collect supernatant cell liquid produced in the stem cell extraction process; the device is provided with scales, so that the generated quantity can be well controlled, and the device has the function of setting the reaction time, so that the reaction result can be well controlled.

Description

Stem cell factor extraction device

Technical Field

The utility model relates to the field of cytokine extraction, in particular to a stem cell factor extraction device.

Background

Cytokines are small molecular proteins synthesized and secreted by stem cells and have wide biological activities, and are information transfer substances which are rich in cell activity in supernatant and are called cytokines. Plays an important role in restoring the functions of damaged tissues and cells in the body.

At present, the demand for cytokines is increased, and the way of extracting the cytokines cannot extract the cytokines rapidly, so that a device is needed to obtain stem cell cytokines rapidly.

Disclosure of Invention

The utility model aims to provide a stem cell factor extraction device so as to realize rapid acquisition of stem cell factors.

In order to achieve the above purpose, the technical scheme provided by the utility model is as follows:

a stem cell factor extraction device, characterized in that: comprises a collecting bottle 1, a reaction bottle 2 and an extraction device 3; the lower end of the extraction device 3 is provided with the reaction bottle 2; the extraction device 3 and one side of the reaction bottle 2 are provided with the collecting bottle 1. The collecting bottle 1 and the reaction bottle 2 can be made of transparent materials with certain strength, such as glass and transparent plastics.

Preferably, the collecting bottle 1 comprises a collecting bottle body 101, a collecting opening 102 and a first scale 103; the outer side surface of the collecting bottle body 101 is provided with the first scale 103, and the upper end of the inner side surface is provided with the collecting opening 102.

Preferably, the reaction bottle 2 comprises a reaction bottle body 201, a reaction bottle interface 202 and a second scale 203; the upper end of the reaction bottle body 201 is provided with the reaction bottle interface 202, and the side surface is provided with the second scale 203.

Preferably, the extraction device 3 includes a device housing 301, a reaction bottle connector 302, a mixing disk 303, a hydraulic rod 304, a motor 305, an extraction port 306, a collection pipe 307, a display screen 308, an adjusting key 309, a function key 310, a liquid outlet pipe 311, a controller 312, a battery 313, a charging port 314, a fan-shaped opening 315, and a micropump 316; the upper end of the device housing 301 is provided with the display 308, the adjusting key 309 and the function key 310; the adjusting key 309 and the function key 310 are disposed at the lower end of the display 308; the lower end of the device housing 301 is provided with the reaction bottle connector 302; the middle of the reaction bottle joint 302 is provided with the hydraulic rod 304; the upper end of the hydraulic rod (304) is provided with the motor (305); the lower end of the motor (305) is provided with the mixing disc (303); the mixing disc (303) is provided with the fan-shaped opening (315); the side surface of the upper end of the device shell (301) is provided with the extraction port (306). The scalloped openings 315 allow for adequate solvent mixing on the one hand and do not interfere with the use of the collection tube 307 on the other hand.

Preferably, the extraction device 3 is internally provided with the controller 312, a battery 313 and a micropump 316; the upper end of the micropump 316 is connected with the liquid outlet pipe 311, and the lower end is connected with the collecting pipe 307; one side of the micropump 316 is provided with the controller 312 and a battery 313; the side of the device housing 301 adjacent to the battery 313 is provided with the charging port 314. Supernatant is collected by the collection tube 307 into the collection bottle 1 by the micropump 316.

Compared with the prior art, the utility model has the beneficial effects that:

the utility model can promote stem cells to quickly produce cytokines, and can well collect supernatant cell liquid produced in the stem cell extraction process; the device is provided with scales, so that the generated quantity can be well controlled.

Drawings

FIG. 1 is a schematic diagram of a stem cell factor extraction device;

FIG. 2 is a schematic diagram of the disassembly of parts of a stem cell factor extraction device;

FIG. 3 is a schematic diagram of a stem cell factor extraction device collection bottle;

FIG. 4 is a schematic diagram of a reaction flask of a stem cell factor extraction device;

FIG. 5 is a schematic diagram of an extraction device of the stem cell factor extraction device;

FIG. 6 is a second schematic diagram of an extraction device of the stem cell factor extraction device;

FIG. 7 is a schematic diagram of the internal components of the stem cell factor extractor.

In the figure: 1. a collection bottle; 2. a reaction bottle; 3. an extraction device; 101. collecting a bottle body; 102. a collection port; 103. a first scale; 201. a reaction bottle body; 202. a reaction bottle interface; 203. a second scale; 301. a device housing; 302. a reaction bottle joint; 303. a mixing tray; 304. a hydraulic rod; 305. a motor; 306. an extraction port; 307. a collection pipe; 308. a display screen; 309. adjusting the keys; 310. a function key; 311. a liquid outlet pipe; 312. a controller; 313. a battery; 314. a charging port; 315. a fan-shaped opening; 316. a micropump.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.

As a possible embodiment, the collecting bottle 1 comprises a collecting bottle body 101, a collecting opening 102 and a first scale 103; the outer side surface of the collecting bottle body 101 is provided with the first scale 103, and the upper end of the inner side surface is provided with the collecting opening 102.

As a possible embodiment, the reaction flask 2 includes a reaction flask body 201, a reaction flask interface 202, and a second scale 203; the upper end of the reaction bottle body 201 is provided with the reaction bottle interface 202, and the side surface is provided with the second scale 203.

As a possible embodiment, the extraction device 3 includes a device housing 301, a reaction flask joint 302, a mixing disk 303, a hydraulic rod 304, a motor 305, an extraction port 306, a collection pipe 307, a display screen 308, an adjustment key 309, a function key 310, a liquid outlet pipe 311, a controller 312, a battery 313, a charging port 314, a fan-shaped opening 315, and a micropump 316; the upper end of the device housing 301 is provided with the display 308, the adjusting key 309 and the function key 310; the adjusting key 309 and the function key 310 are disposed at the lower end of the display 308; the lower end of the device housing 301 is provided with the reaction bottle connector 302; the middle of the reaction bottle joint 302 is provided with the hydraulic rod 304; the upper end of the hydraulic rod (304) is provided with the motor (305); the lower end of the motor (305) is provided with the mixing disc (303); the mixing disc (303) is provided with the fan-shaped opening (315); the side surface of the upper end of the device shell (301) is provided with the extraction port (306). The scalloped openings 315 allow for adequate solvent mixing on the one hand and do not interfere with the use of the collection tube 307 on the other hand.

As a possible embodiment, the extraction device 3 is internally provided with the controller 312, a battery 313 and a micropump 316; the upper end of the micropump 316 is connected with the liquid outlet pipe 311, and the lower end is connected with the collecting pipe 307; one side of the micropump 316 is provided with the controller 312 and a battery 313; the side of the device housing 301 adjacent to the battery 313 is provided with the charging port 314. Supernatant is collected by the collection tube 307 into the collection bottle 1 by the micropump 316. The micropump 316 employs a micro vacuum pump of the kamoer model KLVP 3; the controller 312 is of a STM32F030C8T6QFP48 singlechip; the motor 305 is a 42 step motor.

As a possible implementation mode, when in use, firstly, the stem cells in the most vigorous division period are washed for 1-3 times by PBS buffer phosphate buffer solution, pt. 7.4 or so, then the washed stem cells are put into the reaction bottle 2, and then PBS buffer solution or physiological saline is added into the reaction bottle 2; the reaction bottle 2 is assembled on the extraction device 3, the related functions of the extraction device 3 are set, and the mixing disc 303 moves up and down so that stem cells and reaction liquid are fully mixed; at the reaction time, the collection tube 307 starts to aspirate the cell supernatant into the collection bottle 1; finally, the collecting bottle 1 is taken down.

The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present utility model, and are not intended to limit the utility model, and that various changes and modifications may be made therein without departing from the spirit and scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. A stem cell factor extraction device, characterized in that: comprises a collecting bottle (1), a reaction bottle (2) and an extraction device (3); the lower end of the extraction device (3) is provided with the reaction bottle (2); one side of the extraction device (3) and one side of the reaction bottle (2) are provided with the collecting bottle (1); the collecting bottle (1) comprises a collecting bottle body (101), a collecting opening (102) and a first scale (103); the outer side surface of the collecting bottle body (101) is provided with the first scale (103), and the upper end of the inner side surface is provided with the collecting port (102); the reaction bottle (2) comprises a reaction bottle body (201), a reaction bottle interface (202) and a second scale (203); the upper end of the reaction bottle body (201) is provided with the reaction bottle interface (202), and the side surface is provided with the second scale (203).

2. The stem cell factor extraction device according to claim 1, wherein the extraction device (3) comprises a device housing (301), a reaction vial adapter (302), a mixing disk (303), a hydraulic lever (304), a motor (305), an extraction port (306), a collection tube (307), a display screen (308), an adjustment key (309), a function key (310), a drain tube (311), a controller (312), a battery (313), a charging port (314), a fan-shaped opening (315) and a micropump (316); the upper end of the device shell (301) is provided with the display screen (308), the adjusting keys (309) and the function keys (310); the adjusting keys (309) and the function keys (310) are arranged at the lower end of the display screen (308); the lower end of the device shell (301) is provided with the reaction bottle joint (302); the middle of the reaction bottle joint (302) is provided with the hydraulic rod (304); the upper end of the hydraulic rod (304) is provided with the motor (305); the lower end of the motor (305) is provided with the mixing disc (303); the mixing disc (303) is provided with the fan-shaped opening (315); the side surface of the upper end of the device shell (301) is provided with the extraction port (306).

3. The stem cell factor extraction device according to claim 2, wherein the extraction device (3) is internally provided with the controller (312), a battery (313) and a micropump (316); the upper end of the micropump (316) is connected with the liquid outlet pipe (311), and the lower end is connected with the collecting pipe (307); one side of the micropump (316) is provided with the controller (312) and a battery (313); the side of the device housing (301) close to the battery (313) is provided with the charging port (314).