CN219662936U - Plasmid extraction device - Google Patents
Plasmid extraction device Download PDFInfo
- Publication number
- CN219662936U CN219662936U CN202321375823.3U CN202321375823U CN219662936U CN 219662936 U CN219662936 U CN 219662936U CN 202321375823 U CN202321375823 U CN 202321375823U CN 219662936 U CN219662936 U CN 219662936U
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- chromatographic column
- column
- fixed
- plasmid extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 32
- 238000000605 extraction Methods 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 238000000926 separation method Methods 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 abstract description 3
- 239000007858 starting material Substances 0.000 abstract description 3
- 239000011435 rock Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 8
- 239000007787 solid Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model relates to a plasmid extraction device which comprises a preparation box, wherein a chromatographic column is detachably connected in the preparation box, a mounting plate is fixedly connected to the outer surface of the chromatographic column, two fixing plates are fixedly connected in the preparation box, springs are fixedly connected to the opposite sides of the two fixing plates, and arc-shaped plates are fixedly connected to the opposite sides of the two springs. This plasmid extraction device, when the equipment of preparing at preparing the case top is in the during operation, the chromatographic column is located the inside of preparing the case through the mounting panel, two arc plates press from both sides tight spacing with it under the effect of spring can, when need rock the speed that improves solid-liquid separation to the chromatographic column, drive cam rotation transmission through the starter motor for the cam constantly extrudees movable column, and the movable column produces miniature shake under the condition that has the spring damper and drives the annular plate and control and remove, makes the chromatographic column shake slightly in the inside of preparing the case, thereby reaches the purpose that improves extraction speed.
Description
Technical Field
The utility model relates to the technical field of experimental instruments, in particular to a plasmid extraction device.
Background
Plasmids are an extrachromosomal stable genetic element. Plasmids can be artificially and purposefully added to known or unknown DNA fragments in vitro to form recombinant "plasmid DNA", such artificially synthesized new recombinant "plasmid DNA" is then introduced into the bacteria and propagated as the bacteria multiply, and such large quantities of bacteria are collected, and the new recombinant "plasmid DNA" is extracted or isolated from the bacteria by specific methods to obtain relatively large quantities (several micrograms or grams) of new recombinant "plasmid DNA" having biological activity. This recombinant "plasmid DNA" can be used for various scientific experiments.
In the prior art, the plasmid extraction method generally needs multiple centrifugation, wherein the chromatographic column is not firmly fixed when collecting and converging solid and liquid, and the flow speed of the chromatographic column is slower when transferring the solid and liquid, so that the plasmid extraction speed is influenced, and a plasmid extraction device is provided to solve the problems.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides a plasmid extraction device which has the advantages that a chromatographic column is convenient to fix during plasmid extraction, solid and liquid flow into the chromatographic column can vibrate in a miniature mode, the speed of plasmid extraction is improved, and the like, and the problems that the chromatographic column is inconvenient to fix during the existing plasmid extraction, the flowing speed of the solid and liquid after flowing in is slower, and the plasmid extraction speed is influenced are solved.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a plasmid extraction device, includes prepares the case, the inside of preparing the case can be dismantled and be connected with the chromatographic column, the surface fixedly connected with mounting panel of chromatographic column, the inside fixedly connected with quantity of preparing the case is two fixed plate, two the equal fixedly connected with spring of the equal fixedly connected with of fixed plate opposite side, two the equal fixedly connected with arc of spring opposite side, the inside fixedly connected with quantity of preparing the case is two fixed column, two the equal sliding connection in inside of fixed column has the sliding column, two the same annular plate of sliding column relative one end fixedly connected with, the right side the one end fixedly connected with spring damper of annular plate is kept away from to the sliding column, spring damper and the right side inner wall fixedly connected with of preparing the case, the left side inner wall fixedly connected with fixed block of preparing the case, the top fixedly connected with motor of fixed block, the one end fixedly connected with cam of motor output shaft.
Further, the collection box is placed in the preparation box, and the top of the preparation box is fixedly connected with the preparation equipment body.
Further, the bottom fixedly connected with annular rubber piece of mounting panel, two the shape of fixed plate is rectangular plate.
Further, two the relative one side of arc has all inlayed the slipmat, two the slip through-hole has all been seted up to the inside of fixed column, two the slip post is all slided in the inside of two fixed columns through the slip through-hole.
Further, the inside of annular plate has been seted up and has been placed the through-hole, the chromatographic column is located the inside of annular plate through placing the through-hole.
Further, the top fixedly connected with protection casing of fixed block, the motor passes through protection casing and fixed block fixed connection.
Compared with the prior art, the technical scheme of the utility model has the following beneficial effects:
1. this plasmid extraction device, when the equipment of preparing at preparing the case top is in the during operation, the chromatographic column is located the inside of preparing the case through the mounting panel, two arc plates press from both sides tight spacing with it under the effect of spring can, when need rock the speed that improves solid-liquid separation to the chromatographic column, drive cam rotation transmission through the starter motor for the cam constantly extrudees movable column, and the movable column produces miniature shake under the condition that has the spring damper and drives the annular plate and control and remove, makes the chromatographic column shake slightly in the inside of preparing the case, thereby reaches the purpose that improves extraction speed.
2. According to the plasmid extraction device, the anti-slip pad is embedded in the two arc-shaped plates, so that the stability of the two arc-shaped plates is better when the chromatographic column is clamped, and the purpose of fixing is facilitated. Through setting up annular rubber pad in the bottom of mounting panel for the chromatographic column is when rocking about going on, and annular rubber pad can play the effect of filling the clearance, and the stability of aim at chromatographic column when rocking is better.
Drawings
FIG. 1 is a front cross-sectional view of the structure of the present utility model;
fig. 2 is a partial top view of the structure of the present utility model.
In the figure: 1 preparation box, 2 chromatographic column, 3 mounting panel, 4 fixed plate, 5 spring, 6 arc, 7 fixed column, 8 sliding column, 9 annular plate, 10 spring damper, 11 fixed block, 12 motor, 13 cam, 14 collection box.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-2, a plasmid extraction apparatus in this embodiment includes a preparation box 1, a collection box 14 is disposed in the preparation box 1, and a preparation device body is fixedly connected to the top of the preparation box 1.
The inside of preparing case 1 can be dismantled and be connected with chromatographic column 2, and the surface fixedly connected with mounting panel 3 of chromatographic column 2 prepares the inside fixedly connected with quantity of case 1 and is two fixed plates 4, and the bottom fixedly connected with annular rubber piece of mounting panel 3, the shape of two fixed plates 4 is rectangular plate.
The opposite sides of the two fixing plates 4 are fixedly connected with springs 5, the opposite sides of the two springs 5 are fixedly connected with arc plates 6, and the opposite sides of the two arc plates 6 are embedded with anti-slip pads.
The inside fixedly connected with of preparing case 1 is a fixed column 7 of two quantity, and the inside of two fixed columns 7 is equal sliding connection has sliding column 8, and sliding hole has all been seted up to the inside of two fixed columns 7, and two sliding column 8 all slide at the inside of two fixed columns 7 through sliding hole.
The same annular plate 9 is fixedly connected to the opposite ends of the two sliding columns 8, a placement through hole is formed in the annular plate 9, the chromatographic column 2 is located in the annular plate 9 through the placement through hole, a spring damper 10 is fixedly connected to one end, far away from the annular plate 9, of the right side sliding column 8, and the spring damper 10 is fixedly connected with the right side inner wall of the manufacturing box 1.
Prepare the left side inner wall fixedly connected with fixed block 11 of case 1, the top fixedly connected with motor 12 of fixed block 11, the top fixedly connected with protection casing of fixed block 11, motor 12 passes through protection casing and fixed block 11 fixed connection, the one end fixedly connected with cam 13 of motor 12 output shaft.
In this embodiment, through inlay the slipmat in the inside of two arc 6 for two arc 6 are better in the stability when pressing from both sides and getting chromatographic column 2, are convenient for carry out fixed purpose.
In this embodiment, through setting up annular rubber pad in the bottom of mounting panel 3 for chromatographic column 2 is when rocking about going on, and annular rubber pad can play the effect of filling the clearance, and the stability of aim at chromatographic column 2 when rocking is better.
In this embodiment, the collection box 14 is disposed inside the preparation box 1, so that the extracted solid and liquid can be collected by the collection box 14, which is convenient for subsequent analysis and use.
The specific application method of plasmid extraction is a known technical scheme, and the device is not convenient to fix and improve the practicality of the device to design and explain when solid-liquid separation is carried out on the chromatographic column 2, and is used as a part of the extraction flow.
The working principle of the embodiment is as follows:
this plasmid extraction device, when the equipment of making at the top of making case 1 is in the during operation, chromatographic column 2 is located the inside of making case 1 through mounting panel 3, two arc 6 press from both sides tight spacing with it under the effect of spring 5 can, when need shake the speed that improves solid-liquid separation to chromatographic column 2, drive cam 13 rotation transmission through starter motor 12, make cam 13 constantly extrude movable column 8, movable column 8 produces miniature shake under the condition that has spring damper 10 and drives annular plate 9 and move about, make chromatographic column 2 slightly shake the inside of making case 1, thereby reach the purpose that improves the extraction speed.
It should be noted that standard parts used in the application can be purchased from the market, special-shaped parts can be customized according to the description of the specification and the drawing, the specific connection modes of the parts adopt mature bolts, rivets, welding and the like in the prior art, and the machinery, the parts and the equipment adopt the conventional models in the prior art; in addition, the circuit connection is in a conventional connection manner in the prior art, and details not described in detail in this specification belong to the prior art known to those skilled in the art.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A plasmid extraction device comprising a preparation tank (1), characterized in that: the inside of preparing case (1) is dismantled and is connected with chromatographic column (2), the surface fixedly connected with mounting panel (3) of chromatographic column (2), prepare inside fixedly connected with quantity of case (1) is two fixed plate (4), two equal fixedly connected with spring (5) of fixed plate (4) relative one side, two equal fixedly connected with arc (6) of spring (5) relative one side, prepare the inside fixedly connected with quantity of case (1) is two fixed column (7), two equal sliding connection in inside of fixed column (7) has sliding column (8), two sliding column (8) relative one end fixedly connected with same annular plate (9), the right side the one end fixedly connected with spring damper (10) of annular plate (9) are kept away from to sliding column (8), spring damper (10) and prepare the right side inner wall fixedly connected with of case (1), prepare the left side inner wall fixedly connected with fixed block (11) of case (1), fixed block (12) top fixedly connected with cam (12) of motor (12).
2. The plasmid extraction apparatus of claim 1, wherein: the collecting box (14) is arranged in the preparation box (1), and the top of the preparation box (1) is fixedly connected with the preparation equipment body.
3. The plasmid extraction apparatus of claim 1, wherein: the bottom of mounting panel (3) fixedly connected with annular rubber piece, two the shape of fixed plate (4) is rectangular plate.
4. The plasmid extraction apparatus of claim 1, wherein: two the opposite one side of arc (6) has all inlayed the slipmat, and two the slip through-hole has all been seted up to the inside of fixed column (7), two slip post (8) are all through the slip through-hole in the inside slip of two fixed columns (7).
5. The plasmid extraction apparatus of claim 1, wherein: the inside of annular plate (9) has been seted up and has been placed the through-hole, chromatographic column (2) are located the inside of annular plate (9) through placing the through-hole.
6. The plasmid extraction apparatus of claim 1, wherein: the top of fixed block (11) fixedly connected with protection casing, motor (12) are through protection casing and fixed block (11) fixed connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321375823.3U CN219662936U (en) | 2023-06-01 | 2023-06-01 | Plasmid extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321375823.3U CN219662936U (en) | 2023-06-01 | 2023-06-01 | Plasmid extraction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219662936U true CN219662936U (en) | 2023-09-12 |
Family
ID=87895316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321375823.3U Active CN219662936U (en) | 2023-06-01 | 2023-06-01 | Plasmid extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219662936U (en) |
-
2023
- 2023-06-01 CN CN202321375823.3U patent/CN219662936U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205642828U (en) | Spring pressure measurement | |
| CN219662936U (en) | Plasmid extraction device | |
| DE60328523D1 (en) | MICROORGANISM OR CELL SAMPLING METHOD AND MICROORGANISM OR CELL ASSEMBLY DEVICE USED FOR THE PROCESS | |
| CN211550944U (en) | Regulator for life science instrument | |
| CN103923829B (en) | The needle of bar magnet method extraction apparatus and sleeve pipe mobile system are applied with it | |
| CN215978506U (en) | Concrete vibrating device | |
| CN211853404U (en) | Electromagnetic fixing frame | |
| CN209322905U (en) | Nucleic acid-extracting apparatus | |
| CN217605530U (en) | Concrete low temperature test box | |
| CN213895254U (en) | Agricultural wastewater purifier | |
| CN219185891U (en) | Stem cell factor extraction device | |
| CN216504082U (en) | Gear machining grinding device | |
| CN211771137U (en) | Rice bran oil bio-enzyme method degumming deacidification refining equipment | |
| CN220438082U (en) | Detection equipment for biomass material | |
| CN217661522U (en) | Diagnostic reagent purification device | |
| CN220825117U (en) | Special sieve for detecting bonding index | |
| CN219272745U (en) | Powder filling auxiliary device for heating pipe powder filling | |
| CN211010589U (en) | Computer unable adjustment base convenient to use | |
| CN215065620U (en) | Solid system appearance device | |
| CN211284386U (en) | Place stable PCR appearance | |
| CN219757860U (en) | Pretreatment device for detecting illegal additives in health food | |
| CN217377885U (en) | Full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR integrated device | |
| CN217940233U (en) | External paste detects uses water bath | |
| CN209930104U (en) | Motor magnet assembly quality | |
| CN218646648U (en) | Soil sample collector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |