CN217377885U

CN217377885U - Full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR integrated device

Info

Publication number: CN217377885U
Application number: CN202220386561.XU
Authority: CN
Inventors: 张鹏; 江高阳
Original assignee: Jiangsu Xinlanxin Biotechnology Co ltd
Current assignee: Jiangsu Xinlanxin Biotechnology Co ltd
Priority date: 2022-02-24
Filing date: 2022-02-24
Publication date: 2022-09-06
Anticipated expiration: 2032-02-24

Abstract

A full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR integrated device comprises a fixed frame, wherein a groove is formed in the bottom side of the fixed frame, a cavity is formed in the bottom side of the groove of the fixed frame, fixing parts are fixedly connected to two sides of the upper end of the fixed frame, a vibration motor is fixedly connected to the bottom side of the cavity of the fixed frame, a moving frame is fixedly connected to the output end of the vibration motor, in the moving process of an optical element, data collection can be carried out on liquid in a test tube, and meanwhile, due to the fact that a moving block moves in a matched mode, the moving track of an optical coupling element and the moving track of the optical element can be consistent, signal influence can be reduced, and the problem that signal fluctuation influences data collection during collection is solved; meanwhile, the limiting plate and the baffle can be easily detached by pressing the spring thimble, so that the working efficiency is improved, and the problem that the test tube is inconvenient to fix and detach in the working process is solved; the limiting plate is heated by the heating wire mesh at the bottom side of the limiting plate, so that uniform heating can be realized, and the influence on the accuracy of extraction and collection is reduced.

Description

Full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR integrated device

Technical Field

The utility model relates to a fluorescence quantitative PCR appearance technical field, concretely relates to integrative device of full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR.

Background

A full-automatic nucleic acid extractor is a medical scientific research instrument used in the fields of public health and prevention science, and can carry out full-automatic purification of virus total nucleic acid on samples such as plasma blood of animal and plant tissues by carrying a real-time fluorescent quantitative PCR system.

The device for fixing and disassembling the test tube in the working process is inconvenient, heating is not uniform enough, uniform heating cannot be performed, and signal fluctuation is easy to cause insufficient accuracy in data acquisition.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the technical problem who solves: the device for fixing and disassembling the test tube in the working process is inconvenient, heating is not uniform enough, uniform heating cannot be performed, and signal fluctuation is easy to cause the problem of insufficient accuracy of collected data.

The utility model provides a technical scheme that technical problem adopted is: the utility model provides an integrative device of full-automatic nucleic acid extraction and real-time fluorescence ration PCR, includes fixed frame, fixed frame bottom side is seted up flutedly, fixed frame is located the recess bottom side and opens and is equipped with the cavity, the equal fixedly connected with mounting in fixed frame upper end both sides, the cavity bottom side fixedly connected with shock dynamo of fixed frame, shock dynamo's output fixedly connected with removes the frame, remove fixedly connected with limiting plate in the frame.

As a preferred technical scheme, remove frame bottom side fixedly connected with fixture block, it is corresponding all to have seted up through-hole and through-hole on fixture block and the limiting plate, the through-hole in the limiting plate is glued and is connected with the rubber ring, four sides all are seted up flutedly and the recess interpolation has the spring thimble on removing the frame, convenient fixed and dismantlement test tube.

As a preferred technical scheme of the utility model, it has the test tube to peg graft in the through-hole of limiting plate, the contact of test tube upper end has the baffle, evenly set up on the baffle and be provided with the plasticity card in through-hole and the through-hole, and be located four plasticity cards of same through-hole and contact each other, can form sealedly.

As an optimized technical scheme of the utility model, limiting plate bottom side fixedly connected with heating screen, heating screen comprises wire and copper aluminium thin slice, be provided with the first screw rod of output fixedly connected with of driving piece and driving piece in the cavity of fixed frame bottom side, threaded connection has optical element on the first screw rod, the convenient heating.

As a preferred technical scheme of the utility model, one of them be provided with driving piece and driving piece rotation in the mounting and be connected with the second screw rod, threaded connection has the movable block on the second screw rod, fixedly connected with electric putter on the movable block, the movable block bottom side is provided with the extractor, the side is glued and is connected with the opto-coupler component in the extractor bottom, can conveniently gather data, and the reduction signal influences.

The utility model has the advantages of it is following: in the moving process of the optical element, data collection can be carried out on liquid in the test tube, and meanwhile, the moving block moves in a matching mode, so that the moving tracks of the optical coupling element and the optical element can be consistent, the signal influence can be reduced, and the problem that the data collection is influenced by signal fluctuation in the collection process is solved; meanwhile, the limiting plate and the baffle can be easily detached by pressing the spring thimble, so that the working efficiency is improved, and the problem that the test tube is inconvenient to fix and detach in the working process is solved; the limiting plate is heated by the heating wire mesh at the bottom side of the limiting plate, so that uniform heating can be realized, and the influence on the accuracy of extraction and collection is reduced.

Drawings

FIG. 1 is a schematic side view, cross-sectional structure of a full-automatic integrated apparatus for nucleic acid extraction and real-time fluorescence quantitative PCR according to a preferred embodiment of the present invention;

FIG. 2 is a schematic three-dimensional structure diagram of an integrated apparatus for full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR according to a preferred embodiment of the present invention;

FIG. 3 is an enlarged schematic view of the position A of the fully automatic nucleic acid extraction and real-time fluorescence quantitative PCR integrated device according to a preferred embodiment of the present invention.

Description of reference numerals: 1. a fixing frame; 2. a fixing member; 3. a vibration motor; 4. moving the frame; 5. a limiting plate; 6. a clamping block; 7. a spring thimble; 8. a test tube; 9. a baffle plate; 10. a plastic card; 11. heating the silk screen; 12. a first screw; 13. an optical element; 14. a second screw; 15. a moving block; 16. an electric push rod; 17. an extractor.

Detailed Description

The technical solution of the present invention will be clearly and completely described with reference to the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The present invention will be further explained with reference to the accompanying drawings.

Please refer to the integrative device of full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR that fig. 1-3 shows in combination, including fixed frame 1, fixed frame 1 bottom side is seted up flutedly, fixed frame 1 is located the recess bottom side and is opened and is equipped with the cavity, the equal fixedly connected with mounting 2 in fixed frame 1 upper end both sides, set up driving motor in the mounting 2, can conveniently drive second screw 14 and rotate, the cavity bottom side fixedly connected with vibrating motor 3 of fixed frame 1, the square can drive and remove frame 4 and reciprocate, thereby can stir the liquid in the test tube 8, vibrating motor 3's output end fixedly connected with removes frame 4, fixedly connected with limiting plate 5 in the removal frame 4, the upper end that conveniently seals test tube 8 seals, convenient dismantlement.

Wherein, spring thimble 7 is pegged graft in the recess, and the recess is located four sides on removing frame 4, and in the through-hole of rubber ring rubber connection on limiting plate 5, the through-hole on limiting plate 5 corresponds with the through-hole on

fixture block

6, and 6 fixed connection of fixture block remove frame 4, through pressing down spring thimble 7, can conveniently dismantle test tube 8.

Wherein, plasticity card 10 is provided with four, and four plasticity cards 10 are located same through-hole and contact each other, and the through-hole is located baffle 9, and the contact of baffle 9 has test tube 8, and the test tube 8 is pegged graft in the through-hole of limiting plate 5, and plasticity card 10 contacts each other under the normal condition can form sealedly, and plasticity card 10 possesses certain toughness, can form sealedly.

Wherein, optical element 13 threaded connection has first screw rod 12, the output of first screw rod 12 fixed connection driving piece, and the driving piece is located the cavity of fixed frame 1 bottom side, and heating wire net 11 fixed connection is in the bottom side of limiting plate 5, and heating wire net 11 comprises wire and copper aluminium sheet, can make things convenient for even heating, can drive optical element 13 simultaneously and carry out data acquisition.

Wherein, opto-coupler element glue is connected in extractor 17 bottom side, and extractor 17 is located the movable block 15 bottom side, and electric putter 16 fixed connection is on movable block 15, and movable block 15 threaded connection has second screw rod 14, and second screw rod 14 rotates and is connected with driving piece and is located one of them mounting 2, through optical element 13 cooperation opto-coupler element, can reduce signal interference, promotes the accuracy nature of gathering.

Specifically, the test tube 8 is placed in a through hole of a limiting plate 5 in a movable frame 4, a baffle plate 9 is pressed at the bottom side of a spring thimble 7, so that the test tube can be conveniently fixed, a plastic card 10 corresponds to the test tube 8, a heating silk screen 11 is arranged at the bottom side of the limiting plate 5, the heating silk screen 11 is composed of a copper-aluminum sheet and a lead, so that the test tube 8 can be conveniently and uniformly heated, and the test tube can be fixed and can be conveniently disassembled at the same time through the matching between a clamping block 6 and the baffle plate 9, a driving part in a driving fixing part 2 is driven to enable a second screw 14 to rotate, limiting devices are arranged on the side edges of a movable block 15 and an optical element 13, so that the movable block 15 is in linear motion, an electric push rod 16 is simultaneously started to drive an extractor 17 to move up and down, so that nucleic acid can be extracted, after extraction is completed, the driving part in a cavity is started to drive the first screw 12 to rotate, so that the optical element 13 is in linear motion, thereby can be from 8 bottoms round trip movement of test tube, the opto-coupler component on the cooperation movable block 15 can reduce the signal fluctuation of gathering the in-process, promotes the accuracy nature of data collection greatly.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Other parts not described in detail in the present invention belong to the prior art, and are not described herein again.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.

Claims

1. The utility model provides an integrative device of full-automatic nucleic acid extraction and real-time fluorescence ration PCR, includes fixed frame (1), its characterized in that, fixed frame (1) bottom side is seted up flutedly, fixed frame (1) is located the recess bottom side and has opened and be equipped with the cavity, the equal fixedly connected with mounting (2) in fixed frame (1) upper end both sides, the cavity bottom side fixedly connected with shock dynamo (3) of fixed frame (1), the output end fixedly connected with of shock dynamo (3) removes frame (4), fixedly connected with limiting plate (5) in removal frame (4).

2. The integrated apparatus for full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR according to claim 1, wherein a fixture block (6) is fixedly connected to the bottom side of the movable frame (4), the fixture block (6) and the limiting plate (5) are respectively provided with a through hole and correspond to each other, a rubber ring is connected to the through hole of the limiting plate (5) by glue, and four sides of the movable frame (4) are respectively provided with a groove and spring thimbles (7) are inserted into the grooves.

3. The integrated device for full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR according to claim 2, wherein a test tube (8) is inserted into the through hole of the limiting plate (5), a baffle (9) is contacted with the upper end of the test tube (8), through holes are uniformly formed in the baffle (9), plastic cards (10) are arranged in the through holes, and four plastic cards (10) in the same through hole are contacted with each other.

4. The integrated device for full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR as claimed in claim 1, wherein the bottom side of the limiting plate (5) is fixedly connected with a heating wire mesh (11), the heating wire mesh (11) is composed of a wire and a copper-aluminum sheet, a driving member is arranged in the cavity at the bottom side of the fixing frame (1), the output end of the driving member is fixedly connected with a first screw rod (12), and the first screw rod (12) is in threaded connection with an optical element (13).

5. The integrated device for full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR as claimed in claim 1, wherein a driving member is disposed in one of the fixed members (2) and is rotatably connected with a second screw (14), a moving block (15) is threadedly connected to the second screw (14), an electric push rod (16) is fixedly connected to the moving block (15), an extractor (17) is disposed at a bottom side of the moving block (15), and an optical coupler element is adhesively connected to a bottom side of the extractor (17).